AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1024470

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1024470 MtrunA17_Chr7g0242461 99.216 255 2 0 1 255 1 255 0.0 525
Msa1024470 MtrunA17_Chr4g0006731 63.281 256 92 1 1 254 1 256 8.81e-125 355
Msa1024470 MtrunA17_Chr7g0242451 61.417 254 97 1 1 254 1 253 1.19e-117 337
Msa1024470 MtrunA17_Chr7g0242401 64.167 240 84 2 5 243 32 270 4.68e-112 323
Msa1024470 MtrunA17_Chr3g0113401 57.031 256 108 1 1 254 1 256 4.32e-110 317
Msa1024470 MtrunA17_Chr7g0242431 61.925 239 89 2 6 243 32 269 7.86e-104 302
Msa1024470 MtrunA17_Chr7g0242411 58.750 240 92 4 5 243 1 234 2.12e-96 282
Msa1024470 MtrunA17_Chr7g0242391 55.777 251 105 4 5 250 1 250 9.91e-95 278
Msa1024470 MtrunA17_Chr7g0242441 44.167 240 70 4 5 243 2 178 7.95e-59 185
Msa1024470 MtrunA17_Chr2g0283411 40.566 212 116 3 6 207 4 215 4.44e-53 172
Msa1024470 MtrunA17_Chr2g0283421 40.476 210 117 3 6 207 5 214 9.78e-51 166
Msa1024470 MtrunA17_Chr4g0059001 37.037 189 112 1 27 208 51 239 4.33e-33 121
Msa1024470 MtrunA17_Chr2g0283391 32.759 174 98 7 41 207 1 162 1.22e-18 81.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1024470 AT3G11210.1 62.500 256 94 1 1 254 1 256 1.27e-118 339
Msa1024470 AT2G38180.1 63.274 226 83 0 1 226 1 226 9.24e-107 311
Msa1024470 AT5G62930.1 42.489 233 119 3 5 230 1 225 2.86e-60 191
Msa1024470 AT5G62930.2 42.489 233 119 3 5 230 27 251 6.80e-60 191
Msa1024470 AT5G45920.1 43.333 210 112 3 5 207 2 211 2.77e-54 175
Msa1024470 AT5G45920.4 43.333 210 112 3 5 207 2 211 2.77e-54 175
Msa1024470 AT5G45920.2 43.646 181 95 3 5 178 2 182 1.13e-46 154
Msa1024470 AT5G45920.3 43.646 181 95 3 5 178 2 182 1.13e-46 154
Msa1024470 AT5G62930.3 37.870 169 90 3 69 230 7 167 1.22e-32 118

Find 90 sgRNAs with CRISPR-Local

Find 177 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACTACGTTGTTATCTTATT+TGG 0.199619 7_1:+53796020 None:intergenic
TAATCTCTGGTCTGCAATTC+AGG 0.207060 7_1:-53794086 Msa1024470:CDS
TGGTATTGAAGGAAATATTA+AGG 0.265182 7_1:-53793590 Msa1024470:CDS
ATCAAAACTCATCTGAATTA+TGG 0.265773 7_1:+53795946 None:intergenic
AGACCTCAAATCGTGCTATT+TGG 0.280414 7_1:-53795974 Msa1024470:CDS
TCTGCACTTGGAGGGTAATA+TGG 0.315910 7_1:+53793495 None:intergenic
GCTTCCAATCTGCTTCTCTA+AGG 0.341955 7_1:+53793565 None:intergenic
ATGGAATTCAAGACGTGCTT+TGG 0.369356 7_1:-53795235 Msa1024470:CDS
TGTTGCACTATGTATCATAT+TGG 0.380482 7_1:+53796073 None:intergenic
CTTGATTTGTACTTCACTGA+TGG 0.402939 7_1:+53794350 None:intergenic
AGCTAATTTGTATGCTAGAA+AGG 0.405931 7_1:-53795900 Msa1024470:intron
ATAGTATTGAGGGGATATTC+TGG 0.415164 7_1:-53795258 Msa1024470:CDS
GAGGAGAGGTGAGAAATATA+AGG 0.417441 7_1:+53794373 None:intergenic
ATTTGAAAGTTATTAATCTC+TGG 0.420460 7_1:-53794099 Msa1024470:CDS
TGCTTATGTCCAACCATCAT+TGG 0.435106 7_1:-53794727 Msa1024470:CDS
GAAAGAAAATTTCAATTATG+TGG 0.435591 7_1:-53793286 Msa1024470:three_prime_UTR
TTGGTAATTATCTACTTTGG+TGG 0.439156 7_1:-53794708 Msa1024470:CDS
AGCACGATTTGAGGTCTCAT+TGG 0.440996 7_1:+53795980 None:intergenic
CCCTAGGAGGCATTTGCAAT+GGG 0.445483 7_1:-53793439 Msa1024470:CDS
TCATTGGTAATTATCTACTT+TGG 0.448861 7_1:-53794711 Msa1024470:CDS
TCCCATTGCAAATGCCTCCT+AGG 0.456278 7_1:+53793438 None:intergenic
AGTGACATCCAATGCAAACT+TGG 0.457673 7_1:+53793543 None:intergenic
ACGTGGCAAGGACTGTAAAC+TGG 0.457856 7_1:-53796154 Msa1024470:five_prime_UTR
GTGGTTCCATCTGCACTTGG+AGG 0.459823 7_1:+53793486 None:intergenic
CGCTCCTCAAAGGAAAAGAT+AGG 0.480626 7_1:+53793169 None:intergenic
GTGAAAAGTAAGTCACAGAA+TGG 0.484547 7_1:-53793256 Msa1024470:three_prime_UTR
ATTGGAAGCCAAGTTTGCAT+TGG 0.493472 7_1:-53793551 Msa1024470:CDS
GAATTCAAGACGTGCTTTGG+AGG 0.494234 7_1:-53795232 Msa1024470:CDS
GAGTCCAGTGCAACACAGAC+TGG 0.494319 7_1:-53794184 Msa1024470:intron
GGGTCCTTAGAGAAGCAGAT+TGG 0.495038 7_1:-53793569 Msa1024470:CDS
GGTATTGAAGGAAATATTAA+GGG 0.495090 7_1:-53793589 Msa1024470:CDS
AAGAATATAAAGAAAATATG+AGG 0.495267 7_1:-53794632 Msa1024470:CDS
GATACATAGTGCAACATCTC+AGG 0.495645 7_1:-53796066 Msa1024470:five_prime_UTR
TTGATTTGTACTTCACTGAT+GGG 0.500040 7_1:+53794351 None:intergenic
AGGAAGCAAGGTGGTATTGA+AGG 0.508719 7_1:-53793601 Msa1024470:CDS
GGACCAAGACCAGATGGGTG+GGG 0.511872 7_1:+53794669 None:intergenic
GGAATCACAATTTATAGATG+TGG 0.514147 7_1:+53796094 None:intergenic
AAGTAGATAATTACCAATGA+TGG 0.516176 7_1:+53794714 None:intergenic
ATAGGCGGACATAGTATTGA+GGG 0.529124 7_1:-53795268 Msa1024470:intron
AGGAGTAGTGTTTGAGTGAG+AGG 0.529430 7_1:-53796046 Msa1024470:five_prime_UTR
GAGCCAAATAGCACGATTTG+AGG 0.529805 7_1:+53795971 None:intergenic
GAAGATTGCTAACTACCTAA+AGG 0.534339 7_1:-53794610 Msa1024470:intron
AGGACCAAGACCAGATGGGT+GGG 0.535530 7_1:+53794668 None:intergenic
TGAAAAGTAAGTCACAGAAT+GGG 0.537230 7_1:-53793255 Msa1024470:three_prime_UTR
GACCAAGACCAGATGGGTGG+GGG 0.542601 7_1:+53794670 None:intergenic
CTGATATTTGCATTTATGTG+TGG 0.542844 7_1:-53793397 Msa1024470:three_prime_UTR
GGAGTTCATTTGTCAGCTGA+AGG 0.543643 7_1:-53793621 Msa1024470:CDS
CCCATTGCAAATGCCTCCTA+GGG 0.544514 7_1:+53793439 None:intergenic
GAATCTTCTGCATATTCAGT+TGG 0.544577 7_1:+53793519 None:intergenic
AACATTGTGGAATATATGCT+AGG 0.545833 7_1:-53794150 Msa1024470:CDS
TCCCTAGGAGGCATTTGCAA+TGG 0.552104 7_1:-53793440 Msa1024470:CDS
ACATGAGGACCAAGACCAGA+TGG 0.559879 7_1:+53794663 None:intergenic
ATGTTTCTTACAGTATCCCT+AGG 0.560400 7_1:-53793455 Msa1024470:CDS
TATAGGCGGACATAGTATTG+AGG 0.561827 7_1:-53795269 Msa1024470:intron
GTGGTGGTTCCATCTGCACT+TGG 0.562402 7_1:+53793483 None:intergenic
TATTACCCTCCAAGTGCAGA+TGG 0.563691 7_1:-53793492 Msa1024470:CDS
AAAATCAAAACGCTCCTCAA+AGG 0.564257 7_1:+53793159 None:intergenic
GTCTGCAATTCAGGAAAGAG+AGG 0.567714 7_1:-53794077 Msa1024470:CDS
TATTCTTCAATGGGAACATG+AGG 0.570082 7_1:+53794648 None:intergenic
GGAGTAGTGTTTGAGTGAGA+GGG 0.570999 7_1:-53796045 Msa1024470:five_prime_UTR
AGGACTGTAAACTGGCCCAA+GGG 0.574672 7_1:-53796146 Msa1024470:five_prime_UTR
AAGGACTGTAAACTGGCCCA+AGG 0.574785 7_1:-53796147 Msa1024470:five_prime_UTR
TGGTTCCATCTGCACTTGGA+GGG 0.575597 7_1:+53793487 None:intergenic
ATTTGCATTTATGTGTGGTG+TGG 0.576365 7_1:-53793392 Msa1024470:three_prime_UTR
TACTTCACTGATGGGAGGAG+AGG 0.578122 7_1:+53794359 None:intergenic
GAGGACCAAGACCAGATGGG+TGG 0.585053 7_1:+53794667 None:intergenic
GTAAGAAACATTTATAGTGG+TGG 0.591121 7_1:+53793467 None:intergenic
TATTGAGGGGATATTCTGGA+TGG 0.591373 7_1:-53795254 Msa1024470:CDS
AGATAATTACCAATGATGGT+TGG 0.595168 7_1:+53794718 None:intergenic
GGCAAGCATATAACGTGGCA+AGG 0.597410 7_1:-53796166 Msa1024470:five_prime_UTR
AATATATGCTAGGGCACTGG+TGG 0.598632 7_1:-53794140 Msa1024470:CDS
TTTGTCAGCTGAAGGAAGCA+AGG 0.601085 7_1:-53793613 Msa1024470:CDS
ATTTGTACTTCACTGATGGG+AGG 0.605876 7_1:+53794354 None:intergenic
ACTGTAAGAAACATTTATAG+TGG 0.610031 7_1:+53793464 None:intergenic
CGTAGTAATAAAGACATGGC+AGG 0.612633 7_1:-53796004 Msa1024470:exon
ACATTGTGGAATATATGCTA+GGG 0.622516 7_1:-53794149 Msa1024470:CDS
CAATTCAGGAAAGAGAGGAC+TGG 0.624153 7_1:-53794072 Msa1024470:CDS
GAGAGGTGAGAAATATAAGG+CGG 0.629380 7_1:+53794376 None:intergenic
AAAACTCATCTGAATTATGG+AGG 0.630001 7_1:+53795949 None:intergenic
CATGAGGACCAAGACCAGAT+GGG 0.631173 7_1:+53794664 None:intergenic
TGGAATATATGCTAGGGCAC+TGG 0.636669 7_1:-53794143 Msa1024470:CDS
GGAAGAACAAATGAACATTG+TGG 0.639652 7_1:-53794163 Msa1024470:CDS
TTTCTTACAGTATCCCTAGG+AGG 0.647425 7_1:-53793452 Msa1024470:CDS
GGACTGTAAACTGGCCCAAG+GGG 0.660504 7_1:-53796145 Msa1024470:five_prime_UTR
AAAGGATTAGTTCTTTCACG+TGG 0.671738 7_1:-53793200 Msa1024470:three_prime_UTR
TAGGCGGACATAGTATTGAG+GGG 0.695080 7_1:-53795267 Msa1024470:CDS
ACAACGTAGTAATAAAGACA+TGG 0.695349 7_1:-53796008 Msa1024470:exon
GTCAGCTGAAGGAAGCAAGG+TGG 0.713484 7_1:-53793610 Msa1024470:CDS
ACGTAGGCAAGCATATAACG+TGG 0.760412 7_1:-53796171 None:intergenic
TAAGGCGGATATGATCCGAG+AGG 0.773737 7_1:+53794391 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTTTTGTAATTATTGA+TGG - chr7_1:53793902-53793921 Msa1024470:intron 10.0%
!! TAAAATATTGACATATATAT+AGG - chr7_1:53793949-53793968 Msa1024470:intron 10.0%
!! AAAAAAATTAAATATTCAAG+AGG + chr7_1:53794149-53794168 None:intergenic 10.0%
!!! TTATATTTTTTAATCAACTT+AGG - chr7_1:53794484-53794503 Msa1024470:intron 10.0%
!!! AAATATGATTCATAATTTTT+TGG - chr7_1:53794780-53794799 Msa1024470:intron 10.0%
!!! TTTGATTATTATTTTTTGTT+AGG - chr7_1:53795588-53795607 Msa1024470:intron 10.0%
!!! TTGATTATTATTTTTTGTTA+GGG - chr7_1:53795589-53795608 Msa1024470:intron 10.0%
!!! ATTTTTACTCTTTTAATAGT+TGG + chr7_1:53793676-53793695 None:intergenic 15.0%
!! AGTATAATATATGTAAGAAA+AGG - chr7_1:53793758-53793777 Msa1024470:intron 15.0%
!!! TTAACACATTAATTAGTTTT+AGG - chr7_1:53794262-53794281 Msa1024470:intron 15.0%
!!! TTTTCTTTATATTCTTCAAT+GGG + chr7_1:53794599-53794618 None:intergenic 15.0%
!!! ATTTTCTTTATATTCTTCAA+TGG + chr7_1:53794600-53794619 None:intergenic 15.0%
!! AAGAATATAAAGAAAATATG+AGG - chr7_1:53794603-53794622 Msa1024470:intron 15.0%
!!! TTAATTTGTATACACTATTA+AGG - chr7_1:53794711-53794730 Msa1024470:CDS 15.0%
!!! TTAATTTCTAACATTTGTTA+TGG - chr7_1:53795021-53795040 Msa1024470:intron 15.0%
!!! AATATTCCTTTTAATAATAG+TGG - chr7_1:53795212-53795231 Msa1024470:CDS 15.0%
!! GAAAAAGAAAAAAAAAACTT+TGG - chr7_1:53795892-53795911 Msa1024470:intron 15.0%
!!! ATGTATTTTGAAATAATGAA+AGG - chr7_1:53796017-53796036 Msa1024470:five_prime_UTR 15.0%
!! AATATATGCAAAAATGTACT+TGG + chr7_1:53793444-53793463 None:intergenic 20.0%
!!! GTATATTTTTTGAGTGTTTT+AGG - chr7_1:53793476-53793495 Msa1024470:CDS 20.0%
!! AATATTGACATATATATAGG+CGG - chr7_1:53793952-53793971 Msa1024470:intron 20.0%
!! CAATTATTGGAATATTGAAA+AGG + chr7_1:53794099-53794118 None:intergenic 20.0%
!! TGATTACTAATTACTCATTA+TGG - chr7_1:53794388-53794407 Msa1024470:CDS 20.0%
!! ATCACAAATGTATAATTACT+TGG - chr7_1:53794414-53794433 Msa1024470:intron 20.0%
!! AATATAACAACAAGACAAAA+TGG + chr7_1:53794471-53794490 None:intergenic 20.0%
!! GAAAATCATTATTTACCTTT+AGG + chr7_1:53794643-53794662 None:intergenic 20.0%
!! ATAAATCATAACTTATTGTG+TGG + chr7_1:53794665-53794684 None:intergenic 20.0%
!! ATTTGAAAGTTATTAATCTC+TGG - chr7_1:53795136-53795155 Msa1024470:intron 20.0%
!!! AAAGTACCACTATTATTAAA+AGG + chr7_1:53795221-53795240 None:intergenic 20.0%
!! AAATGTAGAAAAAGTAAAAG+TGG + chr7_1:53795258-53795277 None:intergenic 20.0%
!! TTTGTATAAACAAAAGTTGA+CGG + chr7_1:53795331-53795350 None:intergenic 20.0%
!! TACTTTATCAAATTCATGAA+GGG + chr7_1:53795566-53795585 None:intergenic 20.0%
!! ATACTTTATCAAATTCATGA+AGG + chr7_1:53795567-53795586 None:intergenic 20.0%
!!! TTATTATTTTTTGTTAGGGA+TGG - chr7_1:53795593-53795612 Msa1024470:intron 20.0%
!! GAAAGAAAATTTCAATTATG+TGG - chr7_1:53795949-53795968 Msa1024470:CDS 20.0%
! TACTACGTTGTTATCTTATT+TGG + chr7_1:53793218-53793237 None:intergenic 25.0%
! ATCAAAACTCATCTGAATTA+TGG + chr7_1:53793292-53793311 None:intergenic 25.0%
!!! ATTCAGATGAGTTTTGATAA+TGG - chr7_1:53793294-53793313 Msa1024470:three_prime_UTR 25.0%
!! TCCTACTCTATATCATTTTA+TGG - chr7_1:53793369-53793388 Msa1024470:three_prime_UTR 25.0%
! ACCATAAAATGATATAGAGT+AGG + chr7_1:53793373-53793392 None:intergenic 25.0%
! CCTCATCAATAATATATCTT+TGG + chr7_1:53793571-53793590 None:intergenic 25.0%
! CCAAAGATATATTATTGATG+AGG - chr7_1:53793568-53793587 Msa1024470:CDS 25.0%
!!! ACTAAGAGTTATTTTCTTGT+TGG - chr7_1:53793718-53793737 Msa1024470:intron 25.0%
! GTAAGAAAAGGAGAAATTTA+AGG - chr7_1:53793770-53793789 Msa1024470:intron 25.0%
! AGAATCTATTAGATACCTTA+GGG + chr7_1:53794045-53794064 None:intergenic 25.0%
! TAGAATCTATTAGATACCTT+AGG + chr7_1:53794046-53794065 None:intergenic 25.0%
! AGGTATCTAATAGATTCTAA+TGG - chr7_1:53794047-53794066 Msa1024470:intron 25.0%
!! TATCCTTTTGAATATTCCAT+TGG - chr7_1:53794070-53794089 Msa1024470:CDS 25.0%
!! ATCCTTTTGAATATTCCATT+GGG - chr7_1:53794071-53794090 Msa1024470:CDS 25.0%
! AATATTCAAGAGGATAACAA+TGG + chr7_1:53794139-53794158 None:intergenic 25.0%
! AAGTAGATAATTACCAATGA+TGG + chr7_1:53794524-53794543 None:intergenic 25.0%
!! TCATTGGTAATTATCTACTT+TGG - chr7_1:53794524-53794543 Msa1024470:intron 25.0%
! TACAAATCAAGAAAAAACTC+AGG - chr7_1:53794897-53794916 Msa1024470:intron 25.0%
! AATGTTAAGGAAATAACTGT+AGG + chr7_1:53795287-53795306 None:intergenic 25.0%
! AACAAGATTTGGAAATGTTA+AGG + chr7_1:53795300-53795319 None:intergenic 25.0%
! TAATTTATTCGTCAGATTGT+AGG + chr7_1:53795496-53795515 None:intergenic 25.0%
!! TGGTATTGAAGGAAATATTA+AGG - chr7_1:53795645-53795664 Msa1024470:intron 25.0%
!! GGTATTGAAGGAAATATTAA+GGG - chr7_1:53795646-53795665 Msa1024470:intron 25.0%
! ACTGTAAGAAACATTTATAG+TGG + chr7_1:53795774-53795793 None:intergenic 25.0%
GGAATCACAATTTATAGATG+TGG + chr7_1:53793144-53793163 None:intergenic 30.0%
TGTTGCACTATGTATCATAT+TGG + chr7_1:53793165-53793184 None:intergenic 30.0%
ACAACGTAGTAATAAAGACA+TGG - chr7_1:53793227-53793246 Msa1024470:three_prime_UTR 30.0%
AAAACTCATCTGAATTATGG+AGG + chr7_1:53793289-53793308 None:intergenic 30.0%
! AGCTAATTTGTATGCTAGAA+AGG - chr7_1:53793335-53793354 Msa1024470:three_prime_UTR 30.0%
AGTAGGTAAGAATCAAAATC+TGG + chr7_1:53793595-53793614 None:intergenic 30.0%
CAATTCAGCTTCATTATACT+TGG + chr7_1:53793652-53793671 None:intergenic 30.0%
! ATAAGTCTTTGAGCACTTTT+CGG - chr7_1:53793835-53793854 Msa1024470:intron 30.0%
AACCCAATGGAATATTCAAA+AGG + chr7_1:53794076-53794095 None:intergenic 30.0%
AATATTGAAAAGGAACCCAA+TGG + chr7_1:53794089-53794108 None:intergenic 30.0%
CCAGCTAGCATTAATATTTA+TGG + chr7_1:53794176-53794195 None:intergenic 30.0%
CCATAAATATTAATGCTAGC+TGG - chr7_1:53794173-53794192 Msa1024470:CDS 30.0%
AGATAATTACCAATGATGGT+TGG + chr7_1:53794520-53794539 None:intergenic 30.0%
! TTGGTAATTATCTACTTTGG+TGG - chr7_1:53794527-53794546 Msa1024470:intron 30.0%
!! TTGATTTGTACTTCACTGAT+GGG + chr7_1:53794887-53794906 None:intergenic 30.0%
!! TTCTAACATTTGTTATGGCT+TGG - chr7_1:53795026-53795045 Msa1024470:intron 30.0%
AACATTGTGGAATATATGCT+AGG - chr7_1:53795085-53795104 Msa1024470:intron 30.0%
ACATTGTGGAATATATGCTA+GGG - chr7_1:53795086-53795105 Msa1024470:intron 30.0%
ATCAATGAAGTTGAGTCTAA+CGG - chr7_1:53795458-53795477 Msa1024470:intron 30.0%
GTAAGAAACATTTATAGTGG+TGG + chr7_1:53795771-53795790 None:intergenic 30.0%
CTGATATTTGCATTTATGTG+TGG - chr7_1:53795838-53795857 Msa1024470:intron 30.0%
TGAAAAGTAAGTCACAGAAT+GGG - chr7_1:53795980-53795999 Msa1024470:CDS 30.0%
! ATTTCCTATCTTTTCCTTTG+AGG - chr7_1:53796062-53796081 Msa1024470:five_prime_UTR 30.0%
!! TGATTATTTTTCTGCCCCTT+GGG + chr7_1:53793107-53793126 None:intergenic 35.0%
!! TTGATTATTTTTCTGCCCCT+TGG + chr7_1:53793108-53793127 None:intergenic 35.0%
!!! CAGATGAGTTTTGATAATGG+TGG - chr7_1:53793297-53793316 Msa1024470:three_prime_UTR 35.0%
!!! TGAGTTTTGATAATGGTGGT+TGG - chr7_1:53793301-53793320 Msa1024470:three_prime_UTR 35.0%
!!! GAGTTTTGATAATGGTGGTT+GGG - chr7_1:53793302-53793321 Msa1024470:three_prime_UTR 35.0%
!!! AGTTTTGATAATGGTGGTTG+GGG - chr7_1:53793303-53793322 Msa1024470:three_prime_UTR 35.0%
ATAGTGAAACTTATCCTCCA+AGG - chr7_1:53793518-53793537 Msa1024470:CDS 35.0%
GATACCATTGGATTATACCT+TGG + chr7_1:53793538-53793557 None:intergenic 35.0%
!!! GTTGGAGAATTTTTTGCAGT+AGG + chr7_1:53793612-53793631 None:intergenic 35.0%
! GTGATTAGTTAGTTAGAGGT+TGG + chr7_1:53793630-53793649 None:intergenic 35.0%
! CTTGGTGATTAGTTAGTTAG+AGG + chr7_1:53793634-53793653 None:intergenic 35.0%
! ATAGTATTGAGGGGATATTC+TGG - chr7_1:53793977-53793996 Msa1024470:intron 35.0%
! TCTTGATGAAGTTTTCCCTA+AGG - chr7_1:53794027-53794046 Msa1024470:intron 35.0%
GCAATGTCTACACATCTAAA+TGG - chr7_1:53794210-53794229 Msa1024470:intron 35.0%
TATTCTTCAATGGGAACATG+AGG + chr7_1:53794590-53794609 None:intergenic 35.0%
GAAGATTGCTAACTACCTAA+AGG - chr7_1:53794625-53794644 Msa1024470:CDS 35.0%
!! CTTGATTTGTACTTCACTGA+TGG + chr7_1:53794888-53794907 None:intergenic 35.0%
CAAGAAAAAACTCAGGTTCA+TGG - chr7_1:53794904-53794923 Msa1024470:intron 35.0%
GGAAGAACAAATGAACATTG+TGG - chr7_1:53795072-53795091 Msa1024470:intron 35.0%
GAATCTTCTGCATATTCAGT+TGG + chr7_1:53795719-53795738 None:intergenic 35.0%
ATGTTTCTTACAGTATCCCT+AGG - chr7_1:53795780-53795799 Msa1024470:intron 35.0%
ATTTGCATTTATGTGTGGTG+TGG - chr7_1:53795843-53795862 Msa1024470:intron 35.0%
!! CTGTGACTTACTTTTCACAA+GGG + chr7_1:53795977-53795996 None:intergenic 35.0%
!! TCTGTGACTTACTTTTCACA+AGG + chr7_1:53795978-53795997 None:intergenic 35.0%
GTGAAAAGTAAGTCACAGAA+TGG - chr7_1:53795979-53795998 Msa1024470:CDS 35.0%
!! AAAGGATTAGTTCTTTCACG+TGG - chr7_1:53796035-53796054 Msa1024470:five_prime_UTR 35.0%
AAAATCAAAACGCTCCTCAA+AGG + chr7_1:53796079-53796098 None:intergenic 35.0%
GATACATAGTGCAACATCTC+AGG - chr7_1:53793169-53793188 Msa1024470:three_prime_UTR 40.0%
CGTAGTAATAAAGACATGGC+AGG - chr7_1:53793231-53793250 Msa1024470:three_prime_UTR 40.0%
! AGACCTCAAATCGTGCTATT+TGG - chr7_1:53793261-53793280 Msa1024470:three_prime_UTR 40.0%
TCCTCCAAGGTATAATCCAA+TGG - chr7_1:53793531-53793550 Msa1024470:CDS 40.0%
ACCATTGGATTATACCTTGG+AGG + chr7_1:53793535-53793554 None:intergenic 40.0%
TATAGGCGGACATAGTATTG+AGG - chr7_1:53793966-53793985 Msa1024470:intron 40.0%
! ATAGGCGGACATAGTATTGA+GGG - chr7_1:53793967-53793986 Msa1024470:intron 40.0%
TATTGAGGGGATATTCTGGA+TGG - chr7_1:53793981-53794000 Msa1024470:intron 40.0%
ATGGAATTCAAGACGTGCTT+TGG - chr7_1:53794000-53794019 Msa1024470:intron 40.0%
TGCTTATGTCCAACCATCAT+TGG - chr7_1:53794508-53794527 Msa1024470:intron 40.0%
GAGAGGTGAGAAATATAAGG+CGG + chr7_1:53794862-53794881 None:intergenic 40.0%
GAGGAGAGGTGAGAAATATA+AGG + chr7_1:53794865-53794884 None:intergenic 40.0%
ATTTGTACTTCACTGATGGG+AGG + chr7_1:53794884-53794903 None:intergenic 40.0%
TAATCTCTGGTCTGCAATTC+AGG - chr7_1:53795149-53795168 Msa1024470:intron 40.0%
ATGAAGTTGAGTCTAACGGA+AGG - chr7_1:53795462-53795481 Msa1024470:intron 40.0%
ATTGGAAGCCAAGTTTGCAT+TGG - chr7_1:53795684-53795703 Msa1024470:intron 40.0%
AGTGACATCCAATGCAAACT+TGG + chr7_1:53795695-53795714 None:intergenic 40.0%
TTTCTTACAGTATCCCTAGG+AGG - chr7_1:53795783-53795802 Msa1024470:intron 40.0%
AGGAGTAGTGTTTGAGTGAG+AGG - chr7_1:53793189-53793208 Msa1024470:three_prime_UTR 45.0%
GGAGTAGTGTTTGAGTGAGA+GGG - chr7_1:53793190-53793209 Msa1024470:three_prime_UTR 45.0%
! AGCACGATTTGAGGTCTCAT+TGG + chr7_1:53793258-53793277 None:intergenic 45.0%
GAGCCAAATAGCACGATTTG+AGG + chr7_1:53793267-53793286 None:intergenic 45.0%
GGCGTTTCAAACGATACCAT+TGG + chr7_1:53793550-53793569 None:intergenic 45.0%
! TAGGCGGACATAGTATTGAG+GGG - chr7_1:53793968-53793987 Msa1024470:intron 45.0%
GAATTCAAGACGTGCTTTGG+AGG - chr7_1:53794003-53794022 Msa1024470:intron 45.0%
! CAATGCTTTTCAGAGCCTCT+CGG - chr7_1:53794829-53794848 Msa1024470:intron 45.0%
TGGAATATATGCTAGGGCAC+TGG - chr7_1:53795092-53795111 Msa1024470:intron 45.0%
!! AATATATGCTAGGGCACTGG+TGG - chr7_1:53795095-53795114 Msa1024470:intron 45.0%
GTCTGCAATTCAGGAAAGAG+AGG - chr7_1:53795158-53795177 Msa1024470:intron 45.0%
CAATTCAGGAAAGAGAGGAC+TGG - chr7_1:53795163-53795182 Msa1024470:intron 45.0%
ATGCCACGTAGATGTGAGTT+GGG + chr7_1:53795374-53795393 None:intergenic 45.0%
AATGCCACGTAGATGTGAGT+TGG + chr7_1:53795375-53795394 None:intergenic 45.0%
GGAGTTCATTTGTCAGCTGA+AGG - chr7_1:53795614-53795633 Msa1024470:intron 45.0%
TTTGTCAGCTGAAGGAAGCA+AGG - chr7_1:53795622-53795641 Msa1024470:intron 45.0%
! AGGAAGCAAGGTGGTATTGA+AGG - chr7_1:53795634-53795653 Msa1024470:intron 45.0%
GCTTCCAATCTGCTTCTCTA+AGG + chr7_1:53795673-53795692 None:intergenic 45.0%
TCTGCACTTGGAGGGTAATA+TGG + chr7_1:53795743-53795762 None:intergenic 45.0%
TATTACCCTCCAAGTGCAGA+TGG - chr7_1:53795743-53795762 Msa1024470:intron 45.0%
CGCTCCTCAAAGGAAAAGAT+AGG + chr7_1:53796069-53796088 None:intergenic 45.0%
!! TTAAAATAAAAAACAATTAT+TGG + chr7_1:53794112-53794131 None:intergenic 5.0%
ACGTGGCAAGGACTGTAAAC+TGG - chr7_1:53793081-53793100 Msa1024470:three_prime_UTR 50.0%
AAGGACTGTAAACTGGCCCA+AGG - chr7_1:53793088-53793107 Msa1024470:three_prime_UTR 50.0%
AGGACTGTAAACTGGCCCAA+GGG - chr7_1:53793089-53793108 Msa1024470:three_prime_UTR 50.0%
CATGAGGACCAAGACCAGAT+GGG + chr7_1:53794574-53794593 None:intergenic 50.0%
ACATGAGGACCAAGACCAGA+TGG + chr7_1:53794575-53794594 None:intergenic 50.0%
TAAGGCGGATATGATCCGAG+AGG + chr7_1:53794847-53794866 None:intergenic 50.0%
TACTTCACTGATGGGAGGAG+AGG + chr7_1:53794879-53794898 None:intergenic 50.0%
TCTTCCAGTCTGTGTTGCAC+TGG + chr7_1:53795058-53795077 None:intergenic 50.0%
CGGCAGCAGCTAACAAGATT+TGG + chr7_1:53795311-53795330 None:intergenic 50.0%
TGCCACGTAGATGTGAGTTG+GGG + chr7_1:53795373-53795392 None:intergenic 50.0%
GGGTCCTTAGAGAAGCAGAT+TGG - chr7_1:53795666-53795685 Msa1024470:intron 50.0%
TGGTTCCATCTGCACTTGGA+GGG + chr7_1:53795751-53795770 None:intergenic 50.0%
!! TCCCTAGGAGGCATTTGCAA+TGG - chr7_1:53795795-53795814 Msa1024470:intron 50.0%
CCCATTGCAAATGCCTCCTA+GGG + chr7_1:53795799-53795818 None:intergenic 50.0%
!! CCCTAGGAGGCATTTGCAAT+GGG - chr7_1:53795796-53795815 Msa1024470:intron 50.0%
TCCCATTGCAAATGCCTCCT+AGG + chr7_1:53795800-53795819 None:intergenic 50.0%
GGACTGTAAACTGGCCCAAG+GGG - chr7_1:53793090-53793109 Msa1024470:three_prime_UTR 55.0%
TCAATTCACCCCCACCCATC+TGG - chr7_1:53794557-53794576 Msa1024470:intron 55.0%
AGGACCAAGACCAGATGGGT+GGG + chr7_1:53794570-53794589 None:intergenic 55.0%
GAGTCCAGTGCAACACAGAC+TGG - chr7_1:53795051-53795070 Msa1024470:intron 55.0%
GACCCCAACTCACATCTACG+TGG - chr7_1:53795368-53795387 Msa1024470:intron 55.0%
GTCAGCTGAAGGAAGCAAGG+TGG - chr7_1:53795625-53795644 Msa1024470:intron 55.0%
GTGGTTCCATCTGCACTTGG+AGG + chr7_1:53795752-53795771 None:intergenic 55.0%
GTGGTGGTTCCATCTGCACT+TGG + chr7_1:53795755-53795774 None:intergenic 55.0%
GACCAAGACCAGATGGGTGG+GGG + chr7_1:53794568-53794587 None:intergenic 60.0%
GGACCAAGACCAGATGGGTG+GGG + chr7_1:53794569-53794588 None:intergenic 60.0%
GAGGACCAAGACCAGATGGG+TGG + chr7_1:53794571-53794590 None:intergenic 60.0%
CACCCCCACCCATCTGGTCT+TGG - chr7_1:53794563-53794582 Msa1024470:intron 65.0%
Chromosome Type Strat End Strand Name
chr7_1 gene 53793071 53796186 53793071 ID=Msa1024470;Name=Msa1024470
chr7_1 mRNA 53793071 53796186 53793071 ID=Msa1024470-mRNA-1;Parent=Msa1024470;Name=Msa1024470-mRNA-1;_AED=0.12;_eAED=0.12;_QI=175|1|1|1|1|1|6|360|255
chr7_1 exon 53793071 53793647 53793071 ID=Msa1024470-mRNA-1:exon:10621;Parent=Msa1024470-mRNA-1
chr7_1 exon 53794055 53794198 53794055 ID=Msa1024470-mRNA-1:exon:10620;Parent=Msa1024470-mRNA-1
chr7_1 exon 53794339 53794415 53794339 ID=Msa1024470-mRNA-1:exon:10619;Parent=Msa1024470-mRNA-1
chr7_1 exon 53794611 53794751 53794611 ID=Msa1024470-mRNA-1:exon:10618;Parent=Msa1024470-mRNA-1
chr7_1 exon 53795209 53795286 53795209 ID=Msa1024470-mRNA-1:exon:10617;Parent=Msa1024470-mRNA-1
chr7_1 exon 53795901 53796186 53795901 ID=Msa1024470-mRNA-1:exon:10616;Parent=Msa1024470-mRNA-1
chr7_1 five_prime_UTR 53796012 53796186 53796012 ID=Msa1024470-mRNA-1:five_prime_utr;Parent=Msa1024470-mRNA-1
chr7_1 CDS 53795901 53796011 53795901 ID=Msa1024470-mRNA-1:cds;Parent=Msa1024470-mRNA-1
chr7_1 CDS 53795209 53795286 53795209 ID=Msa1024470-mRNA-1:cds;Parent=Msa1024470-mRNA-1
chr7_1 CDS 53794611 53794751 53794611 ID=Msa1024470-mRNA-1:cds;Parent=Msa1024470-mRNA-1
chr7_1 CDS 53794339 53794415 53794339 ID=Msa1024470-mRNA-1:cds;Parent=Msa1024470-mRNA-1
chr7_1 CDS 53794055 53794198 53794055 ID=Msa1024470-mRNA-1:cds;Parent=Msa1024470-mRNA-1
chr7_1 CDS 53793431 53793647 53793431 ID=Msa1024470-mRNA-1:cds;Parent=Msa1024470-mRNA-1
chr7_1 three_prime_UTR 53793071 53793430 53793071 ID=Msa1024470-mRNA-1:three_prime_utr;Parent=Msa1024470-mRNA-1
Gene Sequence

>Msa1024470

ATGGCAGGACCAATGAGACCTCAAATCGTGCTATTTGGCTCCTCCATAATTCAGATGAGTTTTGATAATGGTGGTTGGGGTGCTATTCTAGCTAATTTGTATGCTAGAAAGGCGGACATAGTATTGAGGGGATATTCTGGATGGAATTCAAGACGTGCTTTGGAGGTTCTTGATGAAGTTTTCCCTAAGGATGCTTATGTCCAACCATCATTGGTAATTATCTACTTTGGTGGAAATGATTCAATTCACCCCCACCCATCTGGTCTTGGTCCTCATGTTCCCATTGAAGAATATAAAGAAAATATGAGGAAGATTGCTAACTACCTAAAGAGCCTCTCGGATCATATCCGCCTTATATTTCTCACCTCTCCTCCCATCAGTGAAGTACAAATCAAGAAAAAACTCAGTGCAACACAGACTGGAAGAACAAATGAACATTGTGGAATATATGCTAGGGCACTGGTGGAACTATGTGATGAGATGAATTTGAAAGTTATTAATCTCTGGTCTGCAATTCAGGAAAGAGAGGACTGGTTAGATGTCAGCTTTACGGATGGAGTTCATTTGTCAGCTGAAGGAAGCAAGGTGGTATTGAAGGAAATATTAAGGGTCCTTAGAGAAGCAGATTGGAAGCCAAGTTTGCATTGGATGTCACTACCAACTGAATATGCAGAAGATTCACCATATTACCCTCCAAGTGCAGATGGAACCACCACTATAAATGTTTCTTACAGTATCCCTAGGAGGCATTTGCAATGGGATTTATAA
Protein sequence

>Msa1024470

MAGPMRPQIVLFGSSIIQMSFDNGGWGAILANLYARKADIVLRGYSGWNSRRALEVLDEVFPKDAYVQPSLVIIYFGGNDSIHPHPSGLGPHVPIEEYKENMRKIANYLKSLSDHIRLIFLTSPPISEVQIKKKLSATQTGRTNEHCGIYARALVELCDEMNLKVINLWSAIQEREDWLDVSFTDGVHLSAEGSKVVLKEILRVLREADWKPSLHWMSLPTEYAEDSPYYPPSADGTTTINVSYSIPRRHLQWDL*