AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1063280

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1063280 MtrunA17_Chr7g0242461 99.216 255 2 0 1 255 1 255 0.0 525
Msa1063280 MtrunA17_Chr4g0006731 63.281 256 92 1 1 254 1 256 8.81e-125 355
Msa1063280 MtrunA17_Chr7g0242451 61.417 254 97 1 1 254 1 253 1.19e-117 337
Msa1063280 MtrunA17_Chr7g0242401 64.167 240 84 2 5 243 32 270 4.68e-112 323
Msa1063280 MtrunA17_Chr3g0113401 57.031 256 108 1 1 254 1 256 4.32e-110 317
Msa1063280 MtrunA17_Chr7g0242431 61.925 239 89 2 6 243 32 269 7.86e-104 302
Msa1063280 MtrunA17_Chr7g0242411 58.750 240 92 4 5 243 1 234 2.12e-96 282
Msa1063280 MtrunA17_Chr7g0242391 55.777 251 105 4 5 250 1 250 9.91e-95 278
Msa1063280 MtrunA17_Chr7g0242441 44.167 240 70 4 5 243 2 178 7.95e-59 185
Msa1063280 MtrunA17_Chr2g0283411 40.566 212 116 3 6 207 4 215 4.44e-53 172
Msa1063280 MtrunA17_Chr2g0283421 40.476 210 117 3 6 207 5 214 9.78e-51 166
Msa1063280 MtrunA17_Chr4g0059001 37.037 189 112 1 27 208 51 239 4.33e-33 121
Msa1063280 MtrunA17_Chr2g0283391 32.759 174 98 7 41 207 1 162 1.22e-18 81.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1063280 AT3G11210.1 62.500 256 94 1 1 254 1 256 1.27e-118 339
Msa1063280 AT2G38180.1 63.274 226 83 0 1 226 1 226 9.24e-107 311
Msa1063280 AT5G62930.1 42.489 233 119 3 5 230 1 225 2.86e-60 191
Msa1063280 AT5G62930.2 42.489 233 119 3 5 230 27 251 6.80e-60 191
Msa1063280 AT5G45920.1 43.333 210 112 3 5 207 2 211 2.77e-54 175
Msa1063280 AT5G45920.4 43.333 210 112 3 5 207 2 211 2.77e-54 175
Msa1063280 AT5G45920.2 43.646 181 95 3 5 178 2 182 1.13e-46 154
Msa1063280 AT5G45920.3 43.646 181 95 3 5 178 2 182 1.13e-46 154
Msa1063280 AT5G62930.3 37.870 169 90 3 69 230 7 167 1.22e-32 118

Find 91 sgRNAs with CRISPR-Local

Find 192 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGTATTGAAGGAAATATTA+AGG 0.265182 7_2:-54929922 Msa1063280:CDS
ATCAAAACTCATCTGAATTA+TGG 0.265773 7_2:+54932420 None:intergenic
AGGAGTAGTGTTTGAGTGAT+AGG 0.268790 7_2:-54932519 Msa1063280:five_prime_UTR
AGACCTCAAATCGTGCTATT+TGG 0.280414 7_2:-54932448 Msa1063280:CDS
TACTACGTTGTTATCTTGTT+TGG 0.311735 7_2:+54932493 None:intergenic
TCTGCACTTGGAGGGTAATA+TGG 0.315910 7_2:+54929827 None:intergenic
CTAACCAGTCCTCTCTTTCC+TGG 0.321965 7_2:+54930413 None:intergenic
GCTTCCAATCTGCTTCTCTA+AGG 0.341955 7_2:+54929897 None:intergenic
ATGGAATTCAAGACGTGCTT+TGG 0.369356 7_2:-54931592 Msa1063280:CDS
TGTTGCACTATGTATCATAT+TGG 0.380482 7_2:+54932546 None:intergenic
TGCTTATGTTCAACCATCAT+TGG 0.391488 7_2:-54931068 Msa1063280:CDS
CTTGATTTGTACTTCACTGA+TGG 0.402939 7_2:+54930696 None:intergenic
AGCTAATTTGTATGCTAGAA+AGG 0.405931 7_2:-54932374 Msa1063280:intron
GAGGAGAGGTGAGAAATATA+AGG 0.417441 7_2:+54930719 None:intergenic
TAATCTCTGGTCTGCAATCC+AGG 0.419500 7_2:-54930431 Msa1063280:CDS
ATTTGAAAGTTATTAATCTC+TGG 0.420460 7_2:-54930444 Msa1063280:CDS
GAAAGAAAATTTCAATTATG+TGG 0.435591 7_2:-54929622 Msa1063280:three_prime_UTR
TTGGTAATTATCTACTTTGG+TGG 0.439156 7_2:-54931049 Msa1063280:CDS
AGCACGATTTGAGGTCTCAT+TGG 0.440996 7_2:+54932454 None:intergenic
CCCTAGGAGGCATTTGCAAT+GGG 0.445483 7_2:-54929771 Msa1063280:CDS
TCATTGGTAATTATCTACTT+TGG 0.448861 7_2:-54931052 Msa1063280:CDS
TCCCATTGCAAATGCCTCCT+AGG 0.456278 7_2:+54929770 None:intergenic
ACGTGGCAAGGACTGTAAAC+TGG 0.457856 7_2:-54932627 Msa1063280:five_prime_UTR
GTGGTTCCATCTGCACTTGG+AGG 0.459823 7_2:+54929818 None:intergenic
AGGGACATCCAATGCAAACT+TGG 0.472340 7_2:+54929875 None:intergenic
GGACCGAGACCAGATGGGTG+GGG 0.475658 7_2:+54931010 None:intergenic
GTGAAAAGTAAGTCACAGAA+TGG 0.484547 7_2:-54929592 Msa1063280:three_prime_UTR
ATTGGAAGCCAAGTTTGCAT+TGG 0.493472 7_2:-54929883 Msa1063280:CDS
GAATTCAAGACGTGCTTTGG+AGG 0.494234 7_2:-54931589 Msa1063280:CDS
CTTCTGCATATTCAGTTGGT+AGG 0.494255 7_2:+54929855 None:intergenic
GAGTCCAGTGCAACACAGAC+TGG 0.494319 7_2:-54930529 Msa1063280:intron
GGGTCCTTAGAGAAGCAGAT+TGG 0.495038 7_2:-54929901 Msa1063280:CDS
GGTATTGAAGGAAATATTAA+GGG 0.495090 7_2:-54929921 Msa1063280:CDS
AAGAATATAAAGAAAATATG+AGG 0.495267 7_2:-54930973 Msa1063280:CDS
GATACATAGTGCAACATCTC+AGG 0.495645 7_2:-54932539 Msa1063280:five_prime_UTR
TTGATTTGTACTTCACTGAT+GGG 0.500040 7_2:+54930697 None:intergenic
GACCGAGACCAGATGGGTGG+GGG 0.501326 7_2:+54931011 None:intergenic
GGAGTAGTGTTTGAGTGATA+GGG 0.504084 7_2:-54932518 Msa1063280:five_prime_UTR
TTCTGCATATTCAGTTGGTA+GGG 0.506516 7_2:+54929856 None:intergenic
AGGAAGCAAGGTGGTATTGA+AGG 0.508719 7_2:-54929933 Msa1063280:CDS
GGAATCACAATTTATAGATG+TGG 0.509177 7_2:+54932567 None:intergenic
AGGACCGAGACCAGATGGGT+GGG 0.512406 7_2:+54931009 None:intergenic
AAGTAGATAATTACCAATGA+TGG 0.516176 7_2:+54931055 None:intergenic
ATAGTATTGAGGGGATACTC+TGG 0.522754 7_2:-54931615 Msa1063280:CDS
ATAGGCGGACATAGTATTGA+GGG 0.529124 7_2:-54931625 Msa1063280:intron
GAGCCAAATAGCACGATTTG+AGG 0.529805 7_2:+54932445 None:intergenic
GAAGATTGCTAACTACCTAA+AGG 0.534339 7_2:-54930951 Msa1063280:intron
TGAAAAGTAAGTCACAGAAT+GGG 0.537230 7_2:-54929591 Msa1063280:three_prime_UTR
ACATGAGGACCGAGACCAGA+TGG 0.542633 7_2:+54931004 None:intergenic
GGAGTTCATTTGTCAGCTGA+AGG 0.543643 7_2:-54929953 Msa1063280:CDS
CCCATTGCAAATGCCTCCTA+GGG 0.544514 7_2:+54929771 None:intergenic
GAATCTTCTGCATATTCAGT+TGG 0.544577 7_2:+54929851 None:intergenic
AACATTGTGGAATATATGCT+AGG 0.545833 7_2:-54930495 Msa1063280:CDS
TCCCTAGGAGGCATTTGCAA+TGG 0.552104 7_2:-54929772 Msa1063280:CDS
GAGGACCGAGACCAGATGGG+TGG 0.559329 7_2:+54931008 None:intergenic
AAAATCAAAACGCTCCTCAA+AGG 0.560195 7_2:+54929493 None:intergenic
ATGTTTCTTACAGTATCCCT+AGG 0.560400 7_2:-54929787 Msa1063280:CDS
TATAGGCGGACATAGTATTG+AGG 0.561827 7_2:-54931626 Msa1063280:intron
GTGGTGGTTCCATCTGCACT+TGG 0.562402 7_2:+54929815 None:intergenic
TATTACCCTCCAAGTGCAGA+TGG 0.563691 7_2:-54929824 Msa1063280:CDS
GTCTGCAATCCAGGAAAGAG+AGG 0.570010 7_2:-54930422 Msa1063280:CDS
TATTCTTCAATGGGAACATG+AGG 0.570082 7_2:+54930989 None:intergenic
AGGACTGTAAACTGGCCCAA+GGG 0.574672 7_2:-54932619 Msa1063280:five_prime_UTR
AAGGACTGTAAACTGGCCCA+AGG 0.574785 7_2:-54932620 Msa1063280:five_prime_UTR
TGGTTCCATCTGCACTTGGA+GGG 0.575597 7_2:+54929819 None:intergenic
TACTTCACTGATGGGAGGAG+AGG 0.578122 7_2:+54930705 None:intergenic
GTAAGAAACATTTATAGTGG+TGG 0.591121 7_2:+54929799 None:intergenic
TATTGAGGGGATACTCTGGA+TGG 0.596383 7_2:-54931611 Msa1063280:CDS
GGCAAGCATATAACGTGGCA+AGG 0.597410 7_2:-54932639 Msa1063280:five_prime_UTR
AATATATGCTAGGGCACTGG+TGG 0.598632 7_2:-54930485 Msa1063280:CDS
TTTGTCAGCTGAAGGAAGCA+AGG 0.601085 7_2:-54929945 Msa1063280:CDS
ATTTGTACTTCACTGATGGG+AGG 0.605876 7_2:+54930700 None:intergenic
ACTGTAAGAAACATTTATAG+TGG 0.610031 7_2:+54929796 None:intergenic
ACGTAGTACAAAGACATGGC+AGG 0.613581 7_2:-54932478 Msa1063280:exon
TATTTGCATTATGTGTGGTG+TGG 0.617253 7_2:-54929726 Msa1063280:three_prime_UTR
ACATTGTGGAATATATGCTA+GGG 0.622516 7_2:-54930494 Msa1063280:CDS
CATGAGGACCGAGACCAGAT+GGG 0.624924 7_2:+54931005 None:intergenic
GAGAGGTGAGAAATATAAGG+CGG 0.629380 7_2:+54930722 None:intergenic
AAAACTCATCTGAATTATGG+AGG 0.630001 7_2:+54932423 None:intergenic
CAATCCAGGAAAGAGAGGAC+TGG 0.632299 7_2:-54930417 Msa1063280:CDS
TGGAATATATGCTAGGGCAC+TGG 0.636669 7_2:-54930488 Msa1063280:CDS
GGAAGAACAAATGAACATTG+TGG 0.639652 7_2:-54930508 Msa1063280:CDS
TTTCTTACAGTATCCCTAGG+AGG 0.647425 7_2:-54929784 Msa1063280:CDS
TCTGATATTTGCATTATGTG+TGG 0.656622 7_2:-54929731 Msa1063280:three_prime_UTR
GGACTGTAAACTGGCCCAAG+GGG 0.660504 7_2:-54932618 Msa1063280:five_prime_UTR
AAAGGATTAGTTCTTTCACG+TGG 0.671738 7_2:-54929536 Msa1063280:three_prime_UTR
TAGGCGGACATAGTATTGAG+GGG 0.695080 7_2:-54931624 Msa1063280:CDS
AACAACGTAGTACAAAGACA+TGG 0.700905 7_2:-54932482 Msa1063280:exon
GTCAGCTGAAGGAAGCAAGG+TGG 0.713484 7_2:-54929942 Msa1063280:CDS
ACGTAGGCAAGCATATAACG+TGG 0.760412 7_2:-54932644 None:intergenic
TAAGGCGGATATGATCCGAG+AGG 0.773737 7_2:+54930737 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATAGACTTTTTTTTTTTTTA+AGG - chr7_2:54930153-54930172 Msa1063280:intron 10.0%
!!! TAAATATGATTTTTCTTTTA+GGG - chr7_2:54930313-54930332 Msa1063280:intron 10.0%
!! TATAATATTGACATATATAT+AGG - chr7_2:54930395-54930414 Msa1063280:intron 10.0%
!!! ACAAATTTTTTTTAAAAAAG+AGG + chr7_2:54930785-54930804 None:intergenic 10.0%
!!! AAATATGATTCATAATTTTT+TGG - chr7_2:54931242-54931261 Msa1063280:intron 10.0%
!!! TTAATTTCTAACATTTTTTA+TGG - chr7_2:54931479-54931498 Msa1063280:intron 10.0%
!!! TTTGATTATTATTTTTTGTT+AGG - chr7_2:54932059-54932078 Msa1063280:intron 10.0%
!!! TTGATTATTATTTTTTGTTA+GGG - chr7_2:54932060-54932079 Msa1063280:intron 10.0%
!! TTGAAAAAAAAAAAAAACTT+TGG - chr7_2:54932359-54932378 Msa1063280:intron 10.0%
!!! ATTTTCACTATTTTAATAGT+TGG + chr7_2:54930003-54930022 None:intergenic 15.0%
!!! ACTTTTTTTTTTTTTAAGGA+AGG - chr7_2:54930157-54930176 Msa1063280:intron 15.0%
!!! TTACATTTGATTTTTCTTTT+AGG - chr7_2:54930266-54930285 Msa1063280:intron 15.0%
!!! TACATTTGATTTTTCTTTTA+GGG - chr7_2:54930267-54930286 Msa1063280:intron 15.0%
!!! GTAAATATGATTTTTCTTTT+AGG - chr7_2:54930312-54930331 Msa1063280:intron 15.0%
!!! TTGTTTTGTTTTGTAAATAT+TGG - chr7_2:54930346-54930365 Msa1063280:intron 15.0%
!!! TTTTTTAAAAAAGAGGTTTT+TGG + chr7_2:54930778-54930797 None:intergenic 15.0%
!!! TTATGTTTTTTAATCAACTT+AGG - chr7_2:54930946-54930965 Msa1063280:intron 15.0%
!!! TTTTCTTTATATTCTTCAAT+GGG + chr7_2:54931061-54931080 None:intergenic 15.0%
!!! ATTTTCTTTATATTCTTCAA+TGG + chr7_2:54931062-54931081 None:intergenic 15.0%
!! AAGAATATAAAGAAAATATG+AGG - chr7_2:54931065-54931084 Msa1063280:CDS 15.0%
!!! TTAATTTGTATACACTATTA+AGG - chr7_2:54931173-54931192 Msa1063280:intron 15.0%
!!! AATATTCCTTTTAATAATAG+TGG - chr7_2:54931670-54931689 Msa1063280:intron 15.0%
!!! ATGTATTTTGAAATAATGAA+AGG - chr7_2:54932484-54932503 Msa1063280:exon 15.0%
!! AATATATGCAAAAATGTACT+TGG + chr7_2:54929771-54929790 None:intergenic 20.0%
!! CCTCATTAATAATATATCTT+CGG + chr7_2:54929898-54929917 None:intergenic 20.0%
!! TAAACAAGATGAAAAATCTA+AGG - chr7_2:54930096-54930115 Msa1063280:intron 20.0%
!!! TTTTGTTTTGTAAATATTGG+TGG - chr7_2:54930349-54930368 Msa1063280:intron 20.0%
!! AATATTGACATATATATAGG+CGG - chr7_2:54930398-54930417 Msa1063280:intron 20.0%
!! CAATTATTGGAATATTGAAA+AGG + chr7_2:54930545-54930564 None:intergenic 20.0%
!! TGATTACTAATTACTCATTA+TGG - chr7_2:54930843-54930862 Msa1063280:intron 20.0%
!! ATCACAAATGTATAATTACT+TGG - chr7_2:54930869-54930888 Msa1063280:intron 20.0%
!! GAAAATCATTATTTACCTTT+AGG + chr7_2:54931105-54931124 None:intergenic 20.0%
!! ATAAATCATAACTTATTGTG+TGG + chr7_2:54931127-54931146 None:intergenic 20.0%
!! ATTTGAAAGTTATTAATCTC+TGG - chr7_2:54931594-54931613 Msa1063280:CDS 20.0%
!!! AAAGTACCACTATTATTAAA+AGG + chr7_2:54931679-54931698 None:intergenic 20.0%
!! AAATGTAGAAAAAGTAAAAG+TGG + chr7_2:54931716-54931735 None:intergenic 20.0%
!! TTTGTATAAACAAAAGTTGA+CGG + chr7_2:54931790-54931809 None:intergenic 20.0%
!! TACTTTATCAAATTCATGAA+GGG + chr7_2:54932037-54932056 None:intergenic 20.0%
!! ATACTTTATCAAATTCATGA+AGG + chr7_2:54932038-54932057 None:intergenic 20.0%
!!! TTATTATTTTTTGTTAGGGA+TGG - chr7_2:54932064-54932083 Msa1063280:intron 20.0%
!! GAAAGAAAATTTCAATTATG+TGG - chr7_2:54932416-54932435 Msa1063280:CDS 20.0%
! ATCAAAACTCATCTGAATTA+TGG + chr7_2:54929621-54929640 None:intergenic 25.0%
!!! ATTCAGATGAGTTTTGATAA+TGG - chr7_2:54929623-54929642 Msa1063280:three_prime_UTR 25.0%
!! TCCTACTCTATATCATTTTA+TGG - chr7_2:54929696-54929715 Msa1063280:three_prime_UTR 25.0%
! ACCATAAAATGATATAGAGT+AGG + chr7_2:54929700-54929719 None:intergenic 25.0%
!! TGCATCTTCTTTTATATTGT+TGG - chr7_2:54929792-54929811 Msa1063280:CDS 25.0%
!!! TTATATTGTTGGAGTGTTTT+AGG - chr7_2:54929803-54929822 Msa1063280:CDS 25.0%
! CCGAAGATATATTATTAATG+AGG - chr7_2:54929895-54929914 Msa1063280:CDS 25.0%
!! TTAGTGATTAGTTAGTTAGA+GGG + chr7_2:54929960-54929979 None:intergenic 25.0%
!!! TAGACGTTTTTTTGTTTTGA+AGG - chr7_2:54930189-54930208 Msa1063280:intron 25.0%
! AGAATCTATTAGATACCTTA+GGG + chr7_2:54930491-54930510 None:intergenic 25.0%
! TAGAATCTATTAGATACCTT+AGG + chr7_2:54930492-54930511 None:intergenic 25.0%
! AGGTATCTAATAGATTCTAA+TGG - chr7_2:54930493-54930512 Msa1063280:CDS 25.0%
!! TATCCTTTTGAATATTCCAT+TGG - chr7_2:54930516-54930535 Msa1063280:CDS 25.0%
!! ATCCTTTTGAATATTCCATT+GGG - chr7_2:54930517-54930536 Msa1063280:CDS 25.0%
! AATATTAAAGAGGATAACGA+TGG + chr7_2:54930585-54930604 None:intergenic 25.0%
! AACATAACAAGAAGACAAAA+TGG + chr7_2:54930933-54930952 None:intergenic 25.0%
! AAGTAGATAATTACCAATGA+TGG + chr7_2:54930986-54931005 None:intergenic 25.0%
!! TCATTGGTAATTATCTACTT+TGG - chr7_2:54930986-54931005 Msa1063280:CDS 25.0%
! TACAAATCAAGAAAAAACTC+AGG - chr7_2:54931354-54931373 Msa1063280:intron 25.0%
!!! TTCTAACATTTTTTATGGCT+TGG - chr7_2:54931484-54931503 Msa1063280:intron 25.0%
! ATGTTAAGGAAAATAACTGT+AGG + chr7_2:54931745-54931764 None:intergenic 25.0%
! AACAAGATTTGGAAATGTTA+AGG + chr7_2:54931759-54931778 None:intergenic 25.0%
! AGCAAAATAATTTATTCGTC+AGG + chr7_2:54931974-54931993 None:intergenic 25.0%
!!! CGAATAAATTATTTTGCTAC+TGG - chr7_2:54931976-54931995 Msa1063280:intron 25.0%
!! TGGTATTGAAGGAAATATTA+AGG - chr7_2:54932116-54932135 Msa1063280:intron 25.0%
!! GGTATTGAAGGAAATATTAA+GGG - chr7_2:54932117-54932136 Msa1063280:intron 25.0%
! ACTGTAAGAAACATTTATAG+TGG + chr7_2:54932245-54932264 None:intergenic 25.0%
GGAATCACAATTTATAGATG+TGG + chr7_2:54929474-54929493 None:intergenic 30.0%
TGTTGCACTATGTATCATAT+TGG + chr7_2:54929495-54929514 None:intergenic 30.0%
TACTACGTTGTTATCTTGTT+TGG + chr7_2:54929548-54929567 None:intergenic 30.0%
AAAACTCATCTGAATTATGG+AGG + chr7_2:54929618-54929637 None:intergenic 30.0%
! AGCTAATTTGTATGCTAGAA+AGG - chr7_2:54929664-54929683 Msa1063280:three_prime_UTR 30.0%
AGTACGTAAGAATCAAAATC+TGG + chr7_2:54929922-54929941 None:intergenic 30.0%
! CTTAGTGATTAGTTAGTTAG+AGG + chr7_2:54929961-54929980 None:intergenic 30.0%
!! ACTAGCAGTTATTTTCTTGT+TGG - chr7_2:54930045-54930064 Msa1063280:intron 30.0%
!!! CGTTTTTTTGTTTTGAAGGA+AGG - chr7_2:54930193-54930212 Msa1063280:intron 30.0%
! ATAAGTCTTTGAGCACTTTT+CGG - chr7_2:54930233-54930252 Msa1063280:intron 30.0%
AACCCAATGGAATATTCAAA+AGG + chr7_2:54930522-54930541 None:intergenic 30.0%
AATATTGAAAAGGAACCCAA+TGG + chr7_2:54930535-54930554 None:intergenic 30.0%
CCAGCTAGCATTAATATTTA+TGG + chr7_2:54930621-54930640 None:intergenic 30.0%
CCATAAATATTAATGCTAGC+TGG - chr7_2:54930618-54930637 Msa1063280:intron 30.0%
! TTGGTAATTATCTACTTTGG+TGG - chr7_2:54930989-54931008 Msa1063280:CDS 30.0%
!! TTGATTTGTACTTCACTGAT+GGG + chr7_2:54931344-54931363 None:intergenic 30.0%
AACATTGTGGAATATATGCT+AGG - chr7_2:54931543-54931562 Msa1063280:intron 30.0%
ACATTGTGGAATATATGCTA+GGG - chr7_2:54931544-54931563 Msa1063280:intron 30.0%
! TAATTTATTCGTCAGGTTGT+AGG + chr7_2:54931967-54931986 None:intergenic 30.0%
GTAAGAAACATTTATAGTGG+TGG + chr7_2:54932242-54932261 None:intergenic 30.0%
TCTGATATTTGCATTATGTG+TGG - chr7_2:54932307-54932326 Msa1063280:intron 30.0%
TGAAAAGTAAGTCACAGAAT+GGG - chr7_2:54932447-54932466 Msa1063280:CDS 30.0%
! TTCCTTATCTTTTTCCTTTG+AGG - chr7_2:54932531-54932550 Msa1063280:five_prime_UTR 30.0%
!! TGATTATTTTTCTGCCCCTT+GGG + chr7_2:54929437-54929456 None:intergenic 35.0%
!! TTGATTATTTTTCTGCCCCT+TGG + chr7_2:54929438-54929457 None:intergenic 35.0%
AACAACGTAGTACAAAGACA+TGG - chr7_2:54929556-54929575 Msa1063280:three_prime_UTR 35.0%
!!! CAGATGAGTTTTGATAATGG+TGG - chr7_2:54929626-54929645 Msa1063280:three_prime_UTR 35.0%
!!! TGAGTTTTGATAATGGTGGT+TGG - chr7_2:54929630-54929649 Msa1063280:three_prime_UTR 35.0%
!!! GAGTTTTGATAATGGTGGTT+GGG - chr7_2:54929631-54929650 Msa1063280:three_prime_UTR 35.0%
!!! AGTTTTGATAATGGTGGTTG+GGG - chr7_2:54929632-54929651 Msa1063280:three_prime_UTR 35.0%
ATAGTGAAACTTATCCTCCA+AGG - chr7_2:54929845-54929864 Msa1063280:CDS 35.0%
CATACCATTGGATTATACCT+TGG + chr7_2:54929865-54929884 None:intergenic 35.0%
CAAGGTATAATCCAATGGTA+TGG - chr7_2:54929863-54929882 Msa1063280:CDS 35.0%
! TCTTGATGAAGTTTTCCCTA+AGG - chr7_2:54930473-54930492 Msa1063280:CDS 35.0%
GCAATGTCTACACATCTAAA+TGG - chr7_2:54930663-54930682 Msa1063280:intron 35.0%
TGCTTATGTTCAACCATCAT+TGG - chr7_2:54930970-54930989 Msa1063280:CDS 35.0%
TATTCTTCAATGGGAACATG+AGG + chr7_2:54931052-54931071 None:intergenic 35.0%
GAAGATTGCTAACTACCTAA+AGG - chr7_2:54931087-54931106 Msa1063280:intron 35.0%
!! CTTGATTTGTACTTCACTGA+TGG + chr7_2:54931345-54931364 None:intergenic 35.0%
CAAGAAAAAACTCAGGTTCA+TGG - chr7_2:54931361-54931380 Msa1063280:intron 35.0%
GGAAGAACAAATGAACATTG+TGG - chr7_2:54931530-54931549 Msa1063280:intron 35.0%
ATCAACGAAGTTGAGTCTAA+CGG - chr7_2:54931929-54931948 Msa1063280:intron 35.0%
! TTCTGCATATTCAGTTGGTA+GGG + chr7_2:54932185-54932204 None:intergenic 35.0%
GAATCTTCTGCATATTCAGT+TGG + chr7_2:54932190-54932209 None:intergenic 35.0%
ATGTTTCTTACAGTATCCCT+AGG - chr7_2:54932251-54932270 Msa1063280:intron 35.0%
TATTTGCATTATGTGTGGTG+TGG - chr7_2:54932312-54932331 Msa1063280:intron 35.0%
!! CTGTGACTTACTTTTCACAA+GGG + chr7_2:54932444-54932463 None:intergenic 35.0%
!! TCTGTGACTTACTTTTCACA+AGG + chr7_2:54932445-54932464 None:intergenic 35.0%
GTGAAAAGTAAGTCACAGAA+TGG - chr7_2:54932446-54932465 Msa1063280:CDS 35.0%
!! AAAGGATTAGTTCTTTCACG+TGG - chr7_2:54932502-54932521 Msa1063280:five_prime_UTR 35.0%
CTCCTCAAAGGAAAAAGATA+AGG + chr7_2:54932536-54932555 None:intergenic 35.0%
AAAATCAAAACGCTCCTCAA+AGG + chr7_2:54932548-54932567 None:intergenic 35.0%
GATACATAGTGCAACATCTC+AGG - chr7_2:54929499-54929518 Msa1063280:three_prime_UTR 40.0%
AGGAGTAGTGTTTGAGTGAT+AGG - chr7_2:54929519-54929538 Msa1063280:three_prime_UTR 40.0%
GGAGTAGTGTTTGAGTGATA+GGG - chr7_2:54929520-54929539 Msa1063280:three_prime_UTR 40.0%
! AGACCTCAAATCGTGCTATT+TGG - chr7_2:54929590-54929609 Msa1063280:three_prime_UTR 40.0%
TCCTCCAAGGTATAATCCAA+TGG - chr7_2:54929858-54929877 Msa1063280:CDS 40.0%
ACCATTGGATTATACCTTGG+AGG + chr7_2:54929862-54929881 None:intergenic 40.0%
GGAGTTTCAAACCATACCAT+TGG + chr7_2:54929877-54929896 None:intergenic 40.0%
! GTGATTAGTTAGTTAGAGGG+TGG + chr7_2:54929957-54929976 None:intergenic 40.0%
TATAGGCGGACATAGTATTG+AGG - chr7_2:54930412-54930431 Msa1063280:CDS 40.0%
! ATAGGCGGACATAGTATTGA+GGG - chr7_2:54930413-54930432 Msa1063280:CDS 40.0%
! ATAGTATTGAGGGGATACTC+TGG - chr7_2:54930423-54930442 Msa1063280:CDS 40.0%
ATGGAATTCAAGACGTGCTT+TGG - chr7_2:54930446-54930465 Msa1063280:CDS 40.0%
GAGAGGTGAGAAATATAAGG+CGG + chr7_2:54931319-54931338 None:intergenic 40.0%
GAGGAGAGGTGAGAAATATA+AGG + chr7_2:54931322-54931341 None:intergenic 40.0%
ATTTGTACTTCACTGATGGG+AGG + chr7_2:54931341-54931360 None:intergenic 40.0%
ATTGGAAGCCAAGTTTGCAT+TGG - chr7_2:54932155-54932174 Msa1063280:intron 40.0%
CTTCTGCATATTCAGTTGGT+AGG + chr7_2:54932186-54932205 None:intergenic 40.0%
TTTCTTACAGTATCCCTAGG+AGG - chr7_2:54932254-54932273 Msa1063280:intron 40.0%
ACGTAGTACAAAGACATGGC+AGG - chr7_2:54929560-54929579 Msa1063280:three_prime_UTR 45.0%
! AGCACGATTTGAGGTCTCAT+TGG + chr7_2:54929587-54929606 None:intergenic 45.0%
GAGCCAAATAGCACGATTTG+AGG + chr7_2:54929596-54929615 None:intergenic 45.0%
! TAGGCGGACATAGTATTGAG+GGG - chr7_2:54930414-54930433 Msa1063280:CDS 45.0%
TATTGAGGGGATACTCTGGA+TGG - chr7_2:54930427-54930446 Msa1063280:CDS 45.0%
GAATTCAAGACGTGCTTTGG+AGG - chr7_2:54930449-54930468 Msa1063280:CDS 45.0%
! CAATGCTTTTCAGAGCCTCT+CGG - chr7_2:54931286-54931305 Msa1063280:intron 45.0%
TGGAATATATGCTAGGGCAC+TGG - chr7_2:54931550-54931569 Msa1063280:intron 45.0%
!! AATATATGCTAGGGCACTGG+TGG - chr7_2:54931553-54931572 Msa1063280:intron 45.0%
TAATCTCTGGTCTGCAATCC+AGG - chr7_2:54931607-54931626 Msa1063280:CDS 45.0%
ACGAAGTTGAGTCTAACGGA+AGG - chr7_2:54931933-54931952 Msa1063280:intron 45.0%
GGAGTTCATTTGTCAGCTGA+AGG - chr7_2:54932085-54932104 Msa1063280:intron 45.0%
TTTGTCAGCTGAAGGAAGCA+AGG - chr7_2:54932093-54932112 Msa1063280:intron 45.0%
! AGGAAGCAAGGTGGTATTGA+AGG - chr7_2:54932105-54932124 Msa1063280:intron 45.0%
GCTTCCAATCTGCTTCTCTA+AGG + chr7_2:54932144-54932163 None:intergenic 45.0%
AGGGACATCCAATGCAAACT+TGG + chr7_2:54932166-54932185 None:intergenic 45.0%
TCTGCACTTGGAGGGTAATA+TGG + chr7_2:54932214-54932233 None:intergenic 45.0%
TATTACCCTCCAAGTGCAGA+TGG - chr7_2:54932214-54932233 Msa1063280:intron 45.0%
!! TTAAAATAAAAAACAATTAT+TGG + chr7_2:54930558-54930577 None:intergenic 5.0%
!! AAAAAAATTAAATATTAAAG+AGG + chr7_2:54930595-54930614 None:intergenic 5.0%
ACGTGGCAAGGACTGTAAAC+TGG - chr7_2:54929411-54929430 Msa1063280:three_prime_UTR 50.0%
AAGGACTGTAAACTGGCCCA+AGG - chr7_2:54929418-54929437 Msa1063280:three_prime_UTR 50.0%
AGGACTGTAAACTGGCCCAA+GGG - chr7_2:54929419-54929438 Msa1063280:three_prime_UTR 50.0%
TAAGGCGGATATGATCCGAG+AGG + chr7_2:54931304-54931323 None:intergenic 50.0%
TACTTCACTGATGGGAGGAG+AGG + chr7_2:54931336-54931355 None:intergenic 50.0%
TCTTCCAGTCTGTGTTGCAC+TGG + chr7_2:54931516-54931535 None:intergenic 50.0%
GTCTGCAATCCAGGAAAGAG+AGG - chr7_2:54931616-54931635 Msa1063280:CDS 50.0%
CAATCCAGGAAAGAGAGGAC+TGG - chr7_2:54931621-54931640 Msa1063280:CDS 50.0%
CTAACCAGTCCTCTCTTTCC+TGG + chr7_2:54931628-54931647 None:intergenic 50.0%
CGGCAGCAGCTAACAAGATT+TGG + chr7_2:54931770-54931789 None:intergenic 50.0%
TGTGAGAGTTGGGGTCTATG+TGG + chr7_2:54931833-54931852 None:intergenic 50.0%
CCACGTAGATGTGAGAGTTG+GGG + chr7_2:54931842-54931861 None:intergenic 50.0%
GCCACGTAGATGTGAGAGTT+GGG + chr7_2:54931843-54931862 None:intergenic 50.0%
TGCCACGTAGATGTGAGAGT+TGG + chr7_2:54931844-54931863 None:intergenic 50.0%
GGGTCCTTAGAGAAGCAGAT+TGG - chr7_2:54932137-54932156 Msa1063280:intron 50.0%
TGGTTCCATCTGCACTTGGA+GGG + chr7_2:54932222-54932241 None:intergenic 50.0%
!! TCCCTAGGAGGCATTTGCAA+TGG - chr7_2:54932266-54932285 Msa1063280:intron 50.0%
CCCATTGCAAATGCCTCCTA+GGG + chr7_2:54932270-54932289 None:intergenic 50.0%
!! CCCTAGGAGGCATTTGCAAT+GGG - chr7_2:54932267-54932286 Msa1063280:intron 50.0%
TCCCATTGCAAATGCCTCCT+AGG + chr7_2:54932271-54932290 None:intergenic 50.0%
GGACTGTAAACTGGCCCAAG+GGG - chr7_2:54929420-54929439 Msa1063280:three_prime_UTR 55.0%
TCAATTCACCCCCACCCATC+TGG - chr7_2:54931019-54931038 Msa1063280:CDS 55.0%
CATGAGGACCGAGACCAGAT+GGG + chr7_2:54931036-54931055 None:intergenic 55.0%
ACATGAGGACCGAGACCAGA+TGG + chr7_2:54931037-54931056 None:intergenic 55.0%
GAGTCCAGTGCAACACAGAC+TGG - chr7_2:54931509-54931528 Msa1063280:intron 55.0%
CCCCAACTCTCACATCTACG+TGG - chr7_2:54931839-54931858 Msa1063280:intron 55.0%
GTCAGCTGAAGGAAGCAAGG+TGG - chr7_2:54932096-54932115 Msa1063280:intron 55.0%
GTGGTTCCATCTGCACTTGG+AGG + chr7_2:54932223-54932242 None:intergenic 55.0%
GTGGTGGTTCCATCTGCACT+TGG + chr7_2:54932226-54932245 None:intergenic 55.0%
AGGACCGAGACCAGATGGGT+GGG + chr7_2:54931032-54931051 None:intergenic 60.0%
CACCCCCACCCATCTGGTCT+CGG - chr7_2:54931025-54931044 Msa1063280:CDS 65.0%
GACCGAGACCAGATGGGTGG+GGG + chr7_2:54931030-54931049 None:intergenic 65.0%
GGACCGAGACCAGATGGGTG+GGG + chr7_2:54931031-54931050 None:intergenic 65.0%
GAGGACCGAGACCAGATGGG+TGG + chr7_2:54931033-54931052 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr7_2 gene 54929401 54932659 54929401 ID=Msa1063280;Name=Msa1063280
chr7_2 mRNA 54929401 54932659 54929401 ID=Msa1063280-mRNA-1;Parent=Msa1063280;Name=Msa1063280-mRNA-1;_AED=0.35;_eAED=0.35;_QI=174|1|1|1|1|1|6|362|255
chr7_2 exon 54929401 54929979 54929401 ID=Msa1063280-mRNA-1:exon:10196;Parent=Msa1063280-mRNA-1
chr7_2 exon 54930400 54930543 54930400 ID=Msa1063280-mRNA-1:exon:10195;Parent=Msa1063280-mRNA-1
chr7_2 exon 54930685 54930761 54930685 ID=Msa1063280-mRNA-1:exon:10194;Parent=Msa1063280-mRNA-1
chr7_2 exon 54930952 54931092 54930952 ID=Msa1063280-mRNA-1:exon:10193;Parent=Msa1063280-mRNA-1
chr7_2 exon 54931566 54931643 54931566 ID=Msa1063280-mRNA-1:exon:10192;Parent=Msa1063280-mRNA-1
chr7_2 exon 54932375 54932659 54932375 ID=Msa1063280-mRNA-1:exon:10191;Parent=Msa1063280-mRNA-1
chr7_2 five_prime_UTR 54932486 54932659 54932486 ID=Msa1063280-mRNA-1:five_prime_utr;Parent=Msa1063280-mRNA-1
chr7_2 CDS 54932375 54932485 54932375 ID=Msa1063280-mRNA-1:cds;Parent=Msa1063280-mRNA-1
chr7_2 CDS 54931566 54931643 54931566 ID=Msa1063280-mRNA-1:cds;Parent=Msa1063280-mRNA-1
chr7_2 CDS 54930952 54931092 54930952 ID=Msa1063280-mRNA-1:cds;Parent=Msa1063280-mRNA-1
chr7_2 CDS 54930685 54930761 54930685 ID=Msa1063280-mRNA-1:cds;Parent=Msa1063280-mRNA-1
chr7_2 CDS 54930400 54930543 54930400 ID=Msa1063280-mRNA-1:cds;Parent=Msa1063280-mRNA-1
chr7_2 CDS 54929763 54929979 54929763 ID=Msa1063280-mRNA-1:cds;Parent=Msa1063280-mRNA-1
chr7_2 three_prime_UTR 54929401 54929762 54929401 ID=Msa1063280-mRNA-1:three_prime_utr;Parent=Msa1063280-mRNA-1
Gene Sequence

>Msa1063280

ATGGCAGGACCAATGAGACCTCAAATCGTGCTATTTGGCTCCTCCATAATTCAGATGAGTTTTGATAATGGTGGTTGGGGTGCTATTCTAGCTAATTTGTATGCTAGAAAGGCGGACATAGTATTGAGGGGATACTCTGGATGGAATTCAAGACGTGCTTTGGAGGTTCTTGATGAAGTTTTCCCTAAGGATGCTTATGTTCAACCATCATTGGTAATTATCTACTTTGGTGGAAATGATTCAATTCACCCCCACCCATCTGGTCTCGGTCCTCATGTTCCCATTGAAGAATATAAAGAAAATATGAGGAAGATTGCTAACTACCTAAAGAGCCTCTCGGATCATATCCGCCTTATATTTCTCACCTCTCCTCCCATCAGTGAAGTACAAATCAAGAAAAAACTCAGTGCAACACAGACTGGAAGAACAAATGAACATTGTGGAATATATGCTAGGGCACTGGTGGAACTATGTGATGAGATGAATTTGAAAGTTATTAATCTCTGGTCTGCAATCCAGGAAAGAGAGGACTGGTTAGATGTCAGCTTTACGGATGGAGTTCATTTGTCAGCTGAAGGAAGCAAGGTGGTATTGAAGGAAATATTAAGGGTCCTTAGAGAAGCAGATTGGAAGCCAAGTTTGCATTGGATGTCCCTACCAACTGAATATGCAGAAGATTCACCATATTACCCTCCAAGTGCAGATGGAACCACCACTATAAATGTTTCTTACAGTATCCCTAGGAGGCATTTGCAATGGGATTTATAA
Protein sequence

>Msa1063280

MAGPMRPQIVLFGSSIIQMSFDNGGWGAILANLYARKADIVLRGYSGWNSRRALEVLDEVFPKDAYVQPSLVIIYFGGNDSIHPHPSGLGPHVPIEEYKENMRKIANYLKSLSDHIRLIFLTSPPISEVQIKKKLSATQTGRTNEHCGIYARALVELCDEMNLKVINLWSAIQEREDWLDVSFTDGVHLSAEGSKVVLKEILRVLREADWKPSLHWMSLPTEYAEDSPYYPPSADGTTTINVSYSIPRRHLQWDL*