AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1027950

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1027950 MtrunA17_Chr5g0411511 92.969 128 9 0 1 128 2 129 8.76e-76 220
Msa1027950 MtrunA17_Chr8g0366891 66.116 121 35 4 3 122 1 116 9.33e-49 152
Msa1027950 MtrunA17_Chr7g0249111 80.597 67 2 1 1 67 1 56 2.09e-22 83.2
Msa1027950 MtrunA17_Chr5g0428121 36.522 115 68 2 16 126 9 122 1.12e-11 57.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1027950 AT5G65660.1 58.268 127 51 2 2 128 1 125 1.45e-45 144

Find 81 sgRNAs with CRISPR-Local

Find 199 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAAAGGGTGGCAAGCAAAA+TGG 0.271852 7_1:-60460976 None:intergenic
GAAGGATTAACTATAACTTA+TGG 0.283702 7_1:-60461018 None:intergenic
TATTACCGTGGAGTGAGATT+TGG 0.322108 7_1:-60461373 None:intergenic
TTGCCACCTGCATAATCCTT+TGG 0.358271 7_1:-60462020 None:intergenic
AAAATACTACTACTCATATA+TGG 0.365240 7_1:-60461067 None:intergenic
GGGATATTTAAACACTTTAG+AGG 0.376848 7_1:-60460899 None:intergenic
AAGGATTAACTATAACTTAT+GGG 0.387964 7_1:-60461017 None:intergenic
ACTTGAGGTGGGGAAAGATC+TGG 0.392706 7_1:-60461171 None:intergenic
CTCATCATCTTCAGCTTAAG+TGG 0.396808 7_1:+60461246 Msa1027950:CDS
TGTAGTTGGTGAAGTGTTGC+GGG 0.397253 7_1:-60460938 None:intergenic
CTTGCCCGTGTCAGCATTCG+CGG 0.412175 7_1:+60461751 Msa1027950:CDS
GGAAAGTAAAGAAACTTTCA+TGG 0.421053 7_1:-60460845 None:intergenic
GTGTGTCGCTGAGGAAATTA+AGG 0.425009 7_1:-60461040 None:intergenic
TTCGTCGGGACAAATGTGTT+TGG 0.425626 7_1:-60461977 None:intergenic
CGAACTGTATTTGTAGTTTG+CGG 0.425667 7_1:+60462125 Msa1027950:three_prime_UTR
GAAAGGGTGGCAAGCAAAAT+GGG 0.433378 7_1:-60460975 None:intergenic
TCTTCTCATGTTGCTACCAC+TGG 0.447898 7_1:+60461271 Msa1027950:CDS
AGTGTGACGGTGTTGATGCC+AGG 0.456740 7_1:+60461699 Msa1027950:CDS
ATGATTTATATGAAATAGAA+AGG 0.457860 7_1:-60460992 None:intergenic
TTGGTGAAGTGTTGCGGGAA+AGG 0.458988 7_1:-60460933 None:intergenic
CAATGGTGATCACGGTGGTT+TGG 0.473390 7_1:-60461811 None:intergenic
GGGTGTATCAACTAGAGAAA+AGG 0.480576 7_1:-60460878 None:intergenic
CCGTCCCTCGGTTTCCCACT+AGG 0.483894 7_1:+60461207 Msa1027950:CDS
AACGAAAAGGATTGTGTAGT+TGG 0.502769 7_1:-60460952 None:intergenic
CGTGGAGTGAGATTTGGACT+TGG 0.508123 7_1:-60461367 None:intergenic
CTTGAGGTGGGGAAAGATCT+GGG 0.508977 7_1:-60461170 None:intergenic
CTTCCTACTGTCTCGTGTGT+AGG 0.509249 7_1:+60462089 Msa1027950:three_prime_UTR
CGAGATGTGTCTACTTGAGG+TGG 0.510749 7_1:-60461183 None:intergenic
AGATTTGGACTTGGAAGGTG+AGG 0.516452 7_1:-60461358 None:intergenic
ATGCTGACACGGGCAAGGCA+TGG 0.518272 7_1:-60461745 None:intergenic
GTGTAGTTGGTGAAGTGTTG+CGG 0.522503 7_1:-60460939 None:intergenic
GGTGTTGATGCCAGGGGATG+AGG 0.522735 7_1:+60461707 Msa1027950:CDS
TCAATCTCTCTCTCATCTTG+AGG 0.522920 7_1:+60461314 Msa1027950:CDS
GACACATCTCGACCGTCCCT+CGG 0.527794 7_1:+60461195 Msa1027950:CDS
AACTTCGGTACCTCATCCCC+TGG 0.529530 7_1:-60461717 None:intergenic
CGCGAATGCTGACACGGGCA+AGG 0.530171 7_1:-60461750 None:intergenic
AGCTGAAGATGATGAGCAAG+AGG 0.534577 7_1:-60461239 None:intergenic
TGGTGGATAGGAGATGGAAG+GGG 0.537876 7_1:+60461662 Msa1027950:intron
TATCAAAGGTAATATGATGA+TGG 0.538330 7_1:+60461143 Msa1027950:exon
CAAGCAAAATGGGAACGAAA+AGG 0.539062 7_1:-60460965 None:intergenic
GGTGATCACGGTGGTTTGGT+CGG 0.540559 7_1:-60461807 None:intergenic
TCTAGCCGCGAATGCTGACA+CGG 0.546125 7_1:-60461756 None:intergenic
CTTCTCATGTTGCTACCACT+GGG 0.549662 7_1:+60461272 Msa1027950:CDS
TTGGTGGATAGGAGATGGAA+GGG 0.551011 7_1:+60461661 Msa1027950:intron
AAAAGACCAAAGGATTATGC+AGG 0.554133 7_1:+60462014 Msa1027950:three_prime_UTR
GGTGGATAGGAGATGGAAGG+GGG 0.554283 7_1:+60461663 Msa1027950:intron
GGATATTTAAACACTTTAGA+GGG 0.560090 7_1:-60460898 None:intergenic
GTTGGTGGATAGGAGATGGA+AGG 0.562698 7_1:+60461660 Msa1027950:intron
GATCACGGTGGTTTGGTCGG+TGG 0.567091 7_1:-60461804 None:intergenic
GGTCGAGATGTGTCTACTTG+AGG 0.570607 7_1:-60461186 None:intergenic
TTTATATGAAATAGAAAGGG+TGG 0.571866 7_1:-60460988 None:intergenic
GTGTTGCGGGAAAGGAAAGT+AGG 0.573190 7_1:-60460925 None:intergenic
ACATACTACAATGGTGATCA+CGG 0.583414 7_1:-60461819 None:intergenic
TAGAGAAAAGGAGCAAGTTG+AGG 0.583790 7_1:-60460866 None:intergenic
GAGGATTGAGTATGTGCTTG+TGG 0.589598 7_1:-60461339 None:intergenic
GAGATGTGTCTACTTGAGGT+GGG 0.592423 7_1:-60461182 None:intergenic
AAAGAAGGGGCAGAGTGTGA+CGG 0.593264 7_1:+60461686 Msa1027950:CDS
AAAGGATTATGCAGGTGGCA+AGG 0.602551 7_1:+60462022 Msa1027950:three_prime_UTR
TGATTTATATGAAATAGAAA+GGG 0.602838 7_1:-60460991 None:intergenic
GCGGGAAAGGAAAGTAGGCG+TGG 0.603747 7_1:-60460920 None:intergenic
CATGTATTCTTCTATACACA+TGG 0.605759 7_1:-60461871 None:intergenic
AGAGGAGAGCAGTACCTAGT+GGG 0.605822 7_1:-60461221 None:intergenic
CTAGCCGCGAATGCTGACAC+GGG 0.608171 7_1:-60461755 None:intergenic
GTGTGACGGTGTTGATGCCA+GGG 0.609269 7_1:+60461700 Msa1027950:CDS
CAAGGCATGGCTATAAACTT+CGG 0.609997 7_1:-60461732 None:intergenic
CGGGAAAGGAAAGTAGGCGT+GGG 0.617775 7_1:-60460919 None:intergenic
GAGTGAGATTTGGACTTGGA+AGG 0.621951 7_1:-60461363 None:intergenic
AAGTTATAAGAGCTTATCAA+AGG 0.623107 7_1:+60461129 Msa1027950:five_prime_UTR
CAGTACCTAGTGGGAAACCG+AGG 0.623236 7_1:-60461212 None:intergenic
AGACCAAAGGATTATGCAGG+TGG 0.626537 7_1:+60462017 Msa1027950:three_prime_UTR
CAAGTCCAAATCTCACTCCA+CGG 0.634220 7_1:+60461368 Msa1027950:CDS
AGATGTGTCTACTTGAGGTG+GGG 0.637020 7_1:-60461181 None:intergenic
AAGAGGAGAGCAGTACCTAG+TGG 0.638253 7_1:-60461222 None:intergenic
GTGCCTACACACGAGACAGT+AGG 0.642189 7_1:-60462092 None:intergenic
GTCGCTGAGGAAATTAAGGA+AGG 0.643661 7_1:-60461036 None:intergenic
CGATAATTAACATACTACAA+TGG 0.657602 7_1:-60461828 None:intergenic
AGTACCTAGTGGGAAACCGA+GGG 0.682252 7_1:-60461211 None:intergenic
CCTAGTGGGAAACCGAGGGA+CGG 0.686417 7_1:-60461207 None:intergenic
TATGGATGTGTGTGTCGCTG+AGG 0.711993 7_1:-60461049 None:intergenic
TGTGACGGTGTTGATGCCAG+GGG 0.749010 7_1:+60461701 Msa1027950:CDS
TACTACAATGGTGATCACGG+TGG 0.756673 7_1:-60461816 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGATTTATATGAAATAGAAA+GGG - chr7_1:60460994-60461013 None:intergenic 15.0%
!! ATGATTTATATGAAATAGAA+AGG - chr7_1:60460995-60461014 None:intergenic 15.0%
!!! AATTGATTATAATTTTCTCT+TGG - chr7_1:60461508-60461527 None:intergenic 15.0%
!!! AATTTTACAATTTTGTTTGT+TGG + chr7_1:60461642-60461661 Msa1027950:intron 15.0%
!!! TTATAATATATCTTTTTCGT+CGG - chr7_1:60461995-60462014 None:intergenic 15.0%
!! ATATATTATAAAAAGACCAA+AGG + chr7_1:60462004-60462023 Msa1027950:three_prime_UTR 15.0%
!! TGATTTATATGAAATAGAAA+GGG - chr7_1:60460994-60461013 None:intergenic 15.0%
!! ATGATTTATATGAAATAGAA+AGG - chr7_1:60460995-60461014 None:intergenic 15.0%
!!! AATTGATTATAATTTTCTCT+TGG - chr7_1:60461508-60461527 None:intergenic 15.0%
!!! AATTTTACAATTTTGTTTGT+TGG + chr7_1:60461642-60461661 Msa1027950:intron 15.0%
!!! TTATAATATATCTTTTTCGT+CGG - chr7_1:60461995-60462014 None:intergenic 15.0%
!! ATATATTATAAAAAGACCAA+AGG + chr7_1:60462004-60462023 Msa1027950:three_prime_UTR 15.0%
!! AAGGATTAACTATAACTTAT+GGG - chr7_1:60461020-60461039 None:intergenic 20.0%
!! AAAATACTACTACTCATATA+TGG - chr7_1:60461070-60461089 None:intergenic 20.0%
!!! TATAATATATCTTTTTCGTC+GGG - chr7_1:60461994-60462013 None:intergenic 20.0%
!! AAGGATTAACTATAACTTAT+GGG - chr7_1:60461020-60461039 None:intergenic 20.0%
!! AAAATACTACTACTCATATA+TGG - chr7_1:60461070-60461089 None:intergenic 20.0%
!!! TATAATATATCTTTTTCGTC+GGG - chr7_1:60461994-60462013 None:intergenic 20.0%
! GGATATTTAAACACTTTAGA+GGG - chr7_1:60460901-60460920 None:intergenic 25.0%
! TTTATATGAAATAGAAAGGG+TGG - chr7_1:60460991-60461010 None:intergenic 25.0%
! GAAGGATTAACTATAACTTA+TGG - chr7_1:60461021-60461040 None:intergenic 25.0%
! AAGTTATAAGAGCTTATCAA+AGG + chr7_1:60461129-60461148 Msa1027950:five_prime_UTR 25.0%
! TATCAAAGGTAATATGATGA+TGG + chr7_1:60461143-60461162 Msa1027950:exon 25.0%
!!! TTTACAATTTTGTTTGTTGG+TGG + chr7_1:60461645-60461664 Msa1027950:intron 25.0%
! CGATAATTAACATACTACAA+TGG - chr7_1:60461831-60461850 None:intergenic 25.0%
! GGATATTTAAACACTTTAGA+GGG - chr7_1:60460901-60460920 None:intergenic 25.0%
! TTTATATGAAATAGAAAGGG+TGG - chr7_1:60460991-60461010 None:intergenic 25.0%
! GAAGGATTAACTATAACTTA+TGG - chr7_1:60461021-60461040 None:intergenic 25.0%
! AAGTTATAAGAGCTTATCAA+AGG + chr7_1:60461129-60461148 Msa1027950:five_prime_UTR 25.0%
! TATCAAAGGTAATATGATGA+TGG + chr7_1:60461143-60461162 Msa1027950:exon 25.0%
!!! TTTACAATTTTGTTTGTTGG+TGG + chr7_1:60461645-60461664 Msa1027950:intron 25.0%
! CGATAATTAACATACTACAA+TGG - chr7_1:60461831-60461850 None:intergenic 25.0%
GGAAAGTAAAGAAACTTTCA+TGG - chr7_1:60460848-60460867 None:intergenic 30.0%
GGGATATTTAAACACTTTAG+AGG - chr7_1:60460902-60460921 None:intergenic 30.0%
AAAGAGTGAAATTATTACCG+TGG - chr7_1:60461388-60461407 None:intergenic 30.0%
AGGATCATACAGTTGTAAAA+TGG - chr7_1:60461547-60461566 None:intergenic 30.0%
TTGACGTTGTAAGTCAAAAA+AGG - chr7_1:60461567-60461586 None:intergenic 30.0%
!! AATTTTGTTTGTTGGTGGAT+AGG + chr7_1:60461650-60461669 Msa1027950:intron 30.0%
CATGTATTCTTCTATACACA+TGG - chr7_1:60461874-60461893 None:intergenic 30.0%
GGAAAGTAAAGAAACTTTCA+TGG - chr7_1:60460848-60460867 None:intergenic 30.0%
GGGATATTTAAACACTTTAG+AGG - chr7_1:60460902-60460921 None:intergenic 30.0%
AAAGAGTGAAATTATTACCG+TGG - chr7_1:60461388-60461407 None:intergenic 30.0%
AGGATCATACAGTTGTAAAA+TGG - chr7_1:60461547-60461566 None:intergenic 30.0%
TTGACGTTGTAAGTCAAAAA+AGG - chr7_1:60461567-60461586 None:intergenic 30.0%
!! AATTTTGTTTGTTGGTGGAT+AGG + chr7_1:60461650-60461669 Msa1027950:intron 30.0%
CATGTATTCTTCTATACACA+TGG - chr7_1:60461874-60461893 None:intergenic 30.0%
AACGAAAAGGATTGTGTAGT+TGG - chr7_1:60460955-60460974 None:intergenic 35.0%
ACATACTACAATGGTGATCA+CGG - chr7_1:60461822-60461841 None:intergenic 35.0%
AAAAGACCAAAGGATTATGC+AGG + chr7_1:60462014-60462033 Msa1027950:three_prime_UTR 35.0%
CGAACTGTATTTGTAGTTTG+CGG + chr7_1:60462125-60462144 Msa1027950:three_prime_UTR 35.0%
AACGAAAAGGATTGTGTAGT+TGG - chr7_1:60460955-60460974 None:intergenic 35.0%
ACATACTACAATGGTGATCA+CGG - chr7_1:60461822-60461841 None:intergenic 35.0%
AAAAGACCAAAGGATTATGC+AGG + chr7_1:60462014-60462033 Msa1027950:three_prime_UTR 35.0%
CGAACTGTATTTGTAGTTTG+CGG + chr7_1:60462125-60462144 Msa1027950:three_prime_UTR 35.0%
TAGAGAAAAGGAGCAAGTTG+AGG - chr7_1:60460869-60460888 None:intergenic 40.0%
GGGTGTATCAACTAGAGAAA+AGG - chr7_1:60460881-60460900 None:intergenic 40.0%
CAAGCAAAATGGGAACGAAA+AGG - chr7_1:60460968-60460987 None:intergenic 40.0%
CTCATCATCTTCAGCTTAAG+TGG + chr7_1:60461246-60461265 Msa1027950:CDS 40.0%
TCAATCTCTCTCTCATCTTG+AGG + chr7_1:60461314-60461333 Msa1027950:CDS 40.0%
TATTACCGTGGAGTGAGATT+TGG - chr7_1:60461376-60461395 None:intergenic 40.0%
CAAGGCATGGCTATAAACTT+CGG - chr7_1:60461735-60461754 None:intergenic 40.0%
TAGAGAAAAGGAGCAAGTTG+AGG - chr7_1:60460869-60460888 None:intergenic 40.0%
GGGTGTATCAACTAGAGAAA+AGG - chr7_1:60460881-60460900 None:intergenic 40.0%
CAAGCAAAATGGGAACGAAA+AGG - chr7_1:60460968-60460987 None:intergenic 40.0%
CTCATCATCTTCAGCTTAAG+TGG + chr7_1:60461246-60461265 Msa1027950:CDS 40.0%
TCAATCTCTCTCTCATCTTG+AGG + chr7_1:60461314-60461333 Msa1027950:CDS 40.0%
TATTACCGTGGAGTGAGATT+TGG - chr7_1:60461376-60461395 None:intergenic 40.0%
CAAGGCATGGCTATAAACTT+CGG - chr7_1:60461735-60461754 None:intergenic 40.0%
!! TGTAGTTGGTGAAGTGTTGC+GGG - chr7_1:60460941-60460960 None:intergenic 45.0%
!! GTGTAGTTGGTGAAGTGTTG+CGG - chr7_1:60460942-60460961 None:intergenic 45.0%
GAAAGGGTGGCAAGCAAAAT+GGG - chr7_1:60460978-60460997 None:intergenic 45.0%
AGAAAGGGTGGCAAGCAAAA+TGG - chr7_1:60460979-60460998 None:intergenic 45.0%
GTCGCTGAGGAAATTAAGGA+AGG - chr7_1:60461039-60461058 None:intergenic 45.0%
! GTGTGTCGCTGAGGAAATTA+AGG - chr7_1:60461043-60461062 None:intergenic 45.0%
!!! TGTTTTGATGCTGATGCTGG+TGG - chr7_1:60461106-60461125 None:intergenic 45.0%
!!! GCTTGTTTTGATGCTGATGC+TGG - chr7_1:60461109-60461128 None:intergenic 45.0%
AGATGTGTCTACTTGAGGTG+GGG - chr7_1:60461184-60461203 None:intergenic 45.0%
GAGATGTGTCTACTTGAGGT+GGG - chr7_1:60461185-60461204 None:intergenic 45.0%
AGCTGAAGATGATGAGCAAG+AGG - chr7_1:60461242-60461261 None:intergenic 45.0%
TCTTCTCATGTTGCTACCAC+TGG + chr7_1:60461271-60461290 Msa1027950:CDS 45.0%
CTTCTCATGTTGCTACCACT+GGG + chr7_1:60461272-60461291 Msa1027950:CDS 45.0%
! GTGAACGAAACTTTTCCCAG+TGG - chr7_1:60461290-60461309 None:intergenic 45.0%
GAGGATTGAGTATGTGCTTG+TGG - chr7_1:60461342-60461361 None:intergenic 45.0%
AGATTTGGACTTGGAAGGTG+AGG - chr7_1:60461361-60461380 None:intergenic 45.0%
GAGTGAGATTTGGACTTGGA+AGG - chr7_1:60461366-60461385 None:intergenic 45.0%
CAAGTCCAAATCTCACTCCA+CGG + chr7_1:60461368-60461387 Msa1027950:CDS 45.0%
! GTTTGTTGGTGGATAGGAGA+TGG + chr7_1:60461656-60461675 Msa1027950:intron 45.0%
! TTGGTGGATAGGAGATGGAA+GGG + chr7_1:60461661-60461680 Msa1027950:intron 45.0%
TACTACAATGGTGATCACGG+TGG - chr7_1:60461819-60461838 None:intergenic 45.0%
TTCGTCGGGACAAATGTGTT+TGG - chr7_1:60461980-60461999 None:intergenic 45.0%
AGACCAAAGGATTATGCAGG+TGG + chr7_1:60462017-60462036 Msa1027950:three_prime_UTR 45.0%
TTGCCACCTGCATAATCCTT+TGG - chr7_1:60462023-60462042 None:intergenic 45.0%
AAAGGATTATGCAGGTGGCA+AGG + chr7_1:60462022-60462041 Msa1027950:three_prime_UTR 45.0%
!! TGTAGTTGGTGAAGTGTTGC+GGG - chr7_1:60460941-60460960 None:intergenic 45.0%
!! GTGTAGTTGGTGAAGTGTTG+CGG - chr7_1:60460942-60460961 None:intergenic 45.0%
GAAAGGGTGGCAAGCAAAAT+GGG - chr7_1:60460978-60460997 None:intergenic 45.0%
AGAAAGGGTGGCAAGCAAAA+TGG - chr7_1:60460979-60460998 None:intergenic 45.0%
GTCGCTGAGGAAATTAAGGA+AGG - chr7_1:60461039-60461058 None:intergenic 45.0%
! GTGTGTCGCTGAGGAAATTA+AGG - chr7_1:60461043-60461062 None:intergenic 45.0%
!!! TGTTTTGATGCTGATGCTGG+TGG - chr7_1:60461106-60461125 None:intergenic 45.0%
!!! GCTTGTTTTGATGCTGATGC+TGG - chr7_1:60461109-60461128 None:intergenic 45.0%
AGATGTGTCTACTTGAGGTG+GGG - chr7_1:60461184-60461203 None:intergenic 45.0%
GAGATGTGTCTACTTGAGGT+GGG - chr7_1:60461185-60461204 None:intergenic 45.0%
AGCTGAAGATGATGAGCAAG+AGG - chr7_1:60461242-60461261 None:intergenic 45.0%
TCTTCTCATGTTGCTACCAC+TGG + chr7_1:60461271-60461290 Msa1027950:CDS 45.0%
CTTCTCATGTTGCTACCACT+GGG + chr7_1:60461272-60461291 Msa1027950:CDS 45.0%
! GTGAACGAAACTTTTCCCAG+TGG - chr7_1:60461290-60461309 None:intergenic 45.0%
GAGGATTGAGTATGTGCTTG+TGG - chr7_1:60461342-60461361 None:intergenic 45.0%
AGATTTGGACTTGGAAGGTG+AGG - chr7_1:60461361-60461380 None:intergenic 45.0%
GAGTGAGATTTGGACTTGGA+AGG - chr7_1:60461366-60461385 None:intergenic 45.0%
CAAGTCCAAATCTCACTCCA+CGG + chr7_1:60461368-60461387 Msa1027950:CDS 45.0%
! GTTTGTTGGTGGATAGGAGA+TGG + chr7_1:60461656-60461675 Msa1027950:intron 45.0%
! TTGGTGGATAGGAGATGGAA+GGG + chr7_1:60461661-60461680 Msa1027950:intron 45.0%
TACTACAATGGTGATCACGG+TGG - chr7_1:60461819-60461838 None:intergenic 45.0%
TTCGTCGGGACAAATGTGTT+TGG - chr7_1:60461980-60461999 None:intergenic 45.0%
AGACCAAAGGATTATGCAGG+TGG + chr7_1:60462017-60462036 Msa1027950:three_prime_UTR 45.0%
TTGCCACCTGCATAATCCTT+TGG - chr7_1:60462023-60462042 None:intergenic 45.0%
AAAGGATTATGCAGGTGGCA+AGG + chr7_1:60462022-60462041 Msa1027950:three_prime_UTR 45.0%
!!! ATAATTTGAAATTATAAAAT+TGG + chr7_1:60461450-60461469 Msa1027950:intron 5.0%
!!! ATAATTTGAAATTATAAAAT+TGG + chr7_1:60461450-60461469 Msa1027950:intron 5.0%
GTGTTGCGGGAAAGGAAAGT+AGG - chr7_1:60460928-60460947 None:intergenic 50.0%
!! TTGGTGAAGTGTTGCGGGAA+AGG - chr7_1:60460936-60460955 None:intergenic 50.0%
! TATGGATGTGTGTGTCGCTG+AGG - chr7_1:60461052-60461071 None:intergenic 50.0%
CTTGAGGTGGGGAAAGATCT+GGG - chr7_1:60461173-60461192 None:intergenic 50.0%
ACTTGAGGTGGGGAAAGATC+TGG - chr7_1:60461174-60461193 None:intergenic 50.0%
CGAGATGTGTCTACTTGAGG+TGG - chr7_1:60461186-60461205 None:intergenic 50.0%
GGTCGAGATGTGTCTACTTG+AGG - chr7_1:60461189-60461208 None:intergenic 50.0%
AGTACCTAGTGGGAAACCGA+GGG - chr7_1:60461214-60461233 None:intergenic 50.0%
AGAGGAGAGCAGTACCTAGT+GGG - chr7_1:60461224-60461243 None:intergenic 50.0%
AAGAGGAGAGCAGTACCTAG+TGG - chr7_1:60461225-60461244 None:intergenic 50.0%
CGTGGAGTGAGATTTGGACT+TGG - chr7_1:60461370-60461389 None:intergenic 50.0%
! GTTGGTGGATAGGAGATGGA+AGG + chr7_1:60461660-60461679 Msa1027950:intron 50.0%
! TGGTGGATAGGAGATGGAAG+GGG + chr7_1:60461662-60461681 Msa1027950:intron 50.0%
GAGATGGAAGGGGGAAAGAA+GGG + chr7_1:60461672-60461691 Msa1027950:CDS 50.0%
AGATGGAAGGGGGAAAGAAG+GGG + chr7_1:60461673-60461692 Msa1027950:CDS 50.0%
AAAGAAGGGGCAGAGTGTGA+CGG + chr7_1:60461686-60461705 Msa1027950:CDS 50.0%
CAATGGTGATCACGGTGGTT+TGG - chr7_1:60461814-60461833 None:intergenic 50.0%
CTTCCTACTGTCTCGTGTGT+AGG + chr7_1:60462089-60462108 Msa1027950:three_prime_UTR 50.0%
GTGTTGCGGGAAAGGAAAGT+AGG - chr7_1:60460928-60460947 None:intergenic 50.0%
!! TTGGTGAAGTGTTGCGGGAA+AGG - chr7_1:60460936-60460955 None:intergenic 50.0%
! TATGGATGTGTGTGTCGCTG+AGG - chr7_1:60461052-60461071 None:intergenic 50.0%
CTTGAGGTGGGGAAAGATCT+GGG - chr7_1:60461173-60461192 None:intergenic 50.0%
ACTTGAGGTGGGGAAAGATC+TGG - chr7_1:60461174-60461193 None:intergenic 50.0%
CGAGATGTGTCTACTTGAGG+TGG - chr7_1:60461186-60461205 None:intergenic 50.0%
GGTCGAGATGTGTCTACTTG+AGG - chr7_1:60461189-60461208 None:intergenic 50.0%
AGTACCTAGTGGGAAACCGA+GGG - chr7_1:60461214-60461233 None:intergenic 50.0%
AGAGGAGAGCAGTACCTAGT+GGG - chr7_1:60461224-60461243 None:intergenic 50.0%
AAGAGGAGAGCAGTACCTAG+TGG - chr7_1:60461225-60461244 None:intergenic 50.0%
CGTGGAGTGAGATTTGGACT+TGG - chr7_1:60461370-60461389 None:intergenic 50.0%
! GTTGGTGGATAGGAGATGGA+AGG + chr7_1:60461660-60461679 Msa1027950:intron 50.0%
! TGGTGGATAGGAGATGGAAG+GGG + chr7_1:60461662-60461681 Msa1027950:intron 50.0%
GAGATGGAAGGGGGAAAGAA+GGG + chr7_1:60461672-60461691 Msa1027950:CDS 50.0%
AGATGGAAGGGGGAAAGAAG+GGG + chr7_1:60461673-60461692 Msa1027950:CDS 50.0%
AAAGAAGGGGCAGAGTGTGA+CGG + chr7_1:60461686-60461705 Msa1027950:CDS 50.0%
CAATGGTGATCACGGTGGTT+TGG - chr7_1:60461814-60461833 None:intergenic 50.0%
CTTCCTACTGTCTCGTGTGT+AGG + chr7_1:60462089-60462108 Msa1027950:three_prime_UTR 50.0%
CGGGAAAGGAAAGTAGGCGT+GGG - chr7_1:60460922-60460941 None:intergenic 55.0%
CAGTACCTAGTGGGAAACCG+AGG - chr7_1:60461215-60461234 None:intergenic 55.0%
GGTGGATAGGAGATGGAAGG+GGG + chr7_1:60461663-60461682 Msa1027950:intron 55.0%
GGAGATGGAAGGGGGAAAGA+AGG + chr7_1:60461671-60461690 Msa1027950:intron 55.0%
!! AGTGTGACGGTGTTGATGCC+AGG + chr7_1:60461699-60461718 Msa1027950:CDS 55.0%
!! GTGTGACGGTGTTGATGCCA+GGG + chr7_1:60461700-60461719 Msa1027950:CDS 55.0%
!! TGTGACGGTGTTGATGCCAG+GGG + chr7_1:60461701-60461720 Msa1027950:CDS 55.0%
AACTTCGGTACCTCATCCCC+TGG - chr7_1:60461720-60461739 None:intergenic 55.0%
TCTAGCCGCGAATGCTGACA+CGG - chr7_1:60461759-60461778 None:intergenic 55.0%
!! GGTGATCACGGTGGTTTGGT+CGG - chr7_1:60461810-60461829 None:intergenic 55.0%
GTGCCTACACACGAGACAGT+AGG - chr7_1:60462095-60462114 None:intergenic 55.0%
TGCGGAGATGCAACTACTGC+TGG + chr7_1:60462143-60462162 Msa1027950:three_prime_UTR 55.0%
CGGGAAAGGAAAGTAGGCGT+GGG - chr7_1:60460922-60460941 None:intergenic 55.0%
CAGTACCTAGTGGGAAACCG+AGG - chr7_1:60461215-60461234 None:intergenic 55.0%
GGTGGATAGGAGATGGAAGG+GGG + chr7_1:60461663-60461682 Msa1027950:intron 55.0%
GGAGATGGAAGGGGGAAAGA+AGG + chr7_1:60461671-60461690 Msa1027950:intron 55.0%
!! AGTGTGACGGTGTTGATGCC+AGG + chr7_1:60461699-60461718 Msa1027950:CDS 55.0%
!! GTGTGACGGTGTTGATGCCA+GGG + chr7_1:60461700-60461719 Msa1027950:CDS 55.0%
!! TGTGACGGTGTTGATGCCAG+GGG + chr7_1:60461701-60461720 Msa1027950:CDS 55.0%
AACTTCGGTACCTCATCCCC+TGG - chr7_1:60461720-60461739 None:intergenic 55.0%
TCTAGCCGCGAATGCTGACA+CGG - chr7_1:60461759-60461778 None:intergenic 55.0%
!! GGTGATCACGGTGGTTTGGT+CGG - chr7_1:60461810-60461829 None:intergenic 55.0%
GTGCCTACACACGAGACAGT+AGG - chr7_1:60462095-60462114 None:intergenic 55.0%
GCGGGAAAGGAAAGTAGGCG+TGG - chr7_1:60460923-60460942 None:intergenic 60.0%
GACACATCTCGACCGTCCCT+CGG + chr7_1:60461195-60461214 Msa1027950:CDS 60.0%
CCTAGTGGGAAACCGAGGGA+CGG - chr7_1:60461210-60461229 None:intergenic 60.0%
!! GGTGTTGATGCCAGGGGATG+AGG + chr7_1:60461707-60461726 Msa1027950:CDS 60.0%
ATGCTGACACGGGCAAGGCA+TGG - chr7_1:60461748-60461767 None:intergenic 60.0%
CTTGCCCGTGTCAGCATTCG+CGG + chr7_1:60461751-60461770 Msa1027950:CDS 60.0%
CTAGCCGCGAATGCTGACAC+GGG - chr7_1:60461758-60461777 None:intergenic 60.0%
!! GATCACGGTGGTTTGGTCGG+TGG - chr7_1:60461807-60461826 None:intergenic 60.0%
GCGGGAAAGGAAAGTAGGCG+TGG - chr7_1:60460923-60460942 None:intergenic 60.0%
GACACATCTCGACCGTCCCT+CGG + chr7_1:60461195-60461214 Msa1027950:CDS 60.0%
CCTAGTGGGAAACCGAGGGA+CGG - chr7_1:60461210-60461229 None:intergenic 60.0%
!! GGTGTTGATGCCAGGGGATG+AGG + chr7_1:60461707-60461726 Msa1027950:CDS 60.0%
ATGCTGACACGGGCAAGGCA+TGG - chr7_1:60461748-60461767 None:intergenic 60.0%
CTTGCCCGTGTCAGCATTCG+CGG + chr7_1:60461751-60461770 Msa1027950:CDS 60.0%
CTAGCCGCGAATGCTGACAC+GGG - chr7_1:60461758-60461777 None:intergenic 60.0%
!! GATCACGGTGGTTTGGTCGG+TGG - chr7_1:60461807-60461826 None:intergenic 60.0%
CCGTCCCTCGGTTTCCCACT+AGG + chr7_1:60461207-60461226 Msa1027950:CDS 65.0%
CGCGAATGCTGACACGGGCA+AGG - chr7_1:60461753-60461772 None:intergenic 65.0%
CCGTCCCTCGGTTTCCCACT+AGG + chr7_1:60461207-60461226 Msa1027950:CDS 65.0%
CGCGAATGCTGACACGGGCA+AGG - chr7_1:60461753-60461772 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr7_1 gene 60460827 60462163 60460827 ID=Msa1027950;Name=Msa1027950
chr7_1 mRNA 60460827 60462163 60460827 ID=Msa1027950-mRNA-1;Parent=Msa1027950;Name=Msa1027950-mRNA-1;_AED=0.00;_eAED=0.00;_QI=329|1|1|1|1|1|2|339|128
chr7_1 exon 60460827 60461389 60460827 ID=Msa1027950-mRNA-1:exon:12503;Parent=Msa1027950-mRNA-1
chr7_1 exon 60461672 60462163 60461672 ID=Msa1027950-mRNA-1:exon:12504;Parent=Msa1027950-mRNA-1
chr7_1 five_prime_UTR 60460827 60461155 60460827 ID=Msa1027950-mRNA-1:five_prime_utr;Parent=Msa1027950-mRNA-1
chr7_1 CDS 60461156 60461389 60461156 ID=Msa1027950-mRNA-1:cds;Parent=Msa1027950-mRNA-1
chr7_1 CDS 60461672 60461824 60461672 ID=Msa1027950-mRNA-1:cds;Parent=Msa1027950-mRNA-1
chr7_1 three_prime_UTR 60461825 60462163 60461825 ID=Msa1027950-mRNA-1:three_prime_utr;Parent=Msa1027950-mRNA-1
Gene Sequence

>Msa1027950

ATGATGATGGAGAGCCCAGATCTTTCCCCACCTCAAGTAGACACATCTCGACCGTCCCTCGGTTTCCCACTAGGTACTGCTCTCCTCTTGCTCATCATCTTCAGCTTAAGTGGTATCTTCTCATGTTGCTACCACTGGGAAAAGTTTCGTTCACTTCATCAATCTCTCTCTCATCTTGAGGCTCCACAAGCACATACTCAATCCTCACCTTCCAAGTCCAAATCTCACTCCACGGAGATGGAAGGGGGAAAGAAGGGGCAGAGTGTGACGGTGTTGATGCCAGGGGATGAGGTACCGAAGTTTATAGCCATGCCTTGCCCGTGTCAGCATTCGCGGCTAGAAGAGATTATTGTCAACATTGAGAAGCCACCGACCAAACCACCGTGA
Protein sequence

>Msa1027950

MMMESPDLSPPQVDTSRPSLGFPLGTALLLLIIFSLSGIFSCCYHWEKFRSLHQSLSHLEAPQAHTQSSPSKSKSHSTEMEGGKKGQSVTVLMPGDEVPKFIAMPCPCQHSRLEEIIVNIEKPPTKPP*