AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1112460

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1112460 MtrunA17_Chr5g0411511 93.750 128 8 0 1 128 2 129 6.80e-77 223
Msa1112460 MtrunA17_Chr8g0366891 66.116 121 35 4 3 122 1 116 2.80e-49 154
Msa1112460 MtrunA17_Chr7g0249111 80.597 67 2 1 1 67 1 56 1.98e-22 83.6
Msa1112460 MtrunA17_Chr5g0428121 36.522 115 68 2 16 126 9 122 4.16e-12 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1112460 AT5G65660.1 58.268 127 51 2 2 128 1 125 5.79e-46 145

Find 83 sgRNAs with CRISPR-Local

Find 197 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAAAGGGTGGCAAGCAAAA+TGG 0.271852 7_3:+58468253 None:intergenic
GAAGGATTAACTATAACTTA+TGG 0.283702 7_3:+58468211 None:intergenic
TATTACCGTGGAGTGAGATT+TGG 0.322108 7_3:+58467856 None:intergenic
TTGCCACCTGCATAATCCTT+TGG 0.358271 7_3:+58467209 None:intergenic
AAAATACTACTACTCATATA+TGG 0.365240 7_3:+58468162 None:intergenic
GGGATATTTAAACACTTTAG+AGG 0.376848 7_3:+58468330 None:intergenic
AAGGATTAACTATAACTTAT+GGG 0.387964 7_3:+58468212 None:intergenic
ACTTGAGGTGGGGAAAGATC+TGG 0.392706 7_3:+58468058 None:intergenic
GGAGATGGAAGGGGAAAAGA+AGG 0.393491 7_3:-58467558 Msa1112460:CDS
CTCATCATCTTCAGCTTAAG+TGG 0.396808 7_3:-58467983 Msa1112460:CDS
TGTAGTTGGTGAAGTGTTGC+GGG 0.397253 7_3:+58468291 None:intergenic
CTTGCCCGTGTCAGCATTCG+CGG 0.412175 7_3:-58467478 Msa1112460:CDS
GGAAAGTAAAGAAACTTTCA+TGG 0.421053 7_3:+58468384 None:intergenic
GTGTGTCGCTGAGGAAATTA+AGG 0.425009 7_3:+58468189 None:intergenic
TTCGTCGGGACAAATGTGTT+TGG 0.425626 7_3:+58467252 None:intergenic
CGAACTGTATTTGTAGTTTG+CGG 0.425667 7_3:-58467104 Msa1112460:three_prime_UTR
GAAAGGGTGGCAAGCAAAAT+GGG 0.433378 7_3:+58468254 None:intergenic
TCTTCTCATGTTGCTACCAC+TGG 0.447898 7_3:-58467958 Msa1112460:CDS
AGTGTGACGGTGTTGATGCC+AGG 0.456740 7_3:-58467530 Msa1112460:CDS
ATGATTTATATGAAATAGAA+AGG 0.457860 7_3:+58468237 None:intergenic
TTGGTGAAGTGTTGCGGGAA+AGG 0.458988 7_3:+58468296 None:intergenic
AAAGAAAAGGATTGTGTAGT+TGG 0.472421 7_3:+58468277 None:intergenic
CAATGGTGATCACGGTGGTT+TGG 0.473390 7_3:+58467418 None:intergenic
GGGTGTATCAACTAGAGAAA+AGG 0.480576 7_3:+58468351 None:intergenic
CCGTCCCTCGGTTTCCCACT+AGG 0.483894 7_3:-58468022 Msa1112460:CDS
CGTGGAGTGAGATTTGGACT+TGG 0.508123 7_3:+58467862 None:intergenic
CTTGAGGTGGGGAAAGATCT+GGG 0.508977 7_3:+58468059 None:intergenic
CTTCCTACTGTCTCGTGTGT+AGG 0.509249 7_3:-58467140 Msa1112460:three_prime_UTR
CGAGATGTGTCTACTTGAGG+TGG 0.510749 7_3:+58468046 None:intergenic
CAAGCAAAATGGGAAAGAAA+AGG 0.516215 7_3:+58468264 None:intergenic
AGATTTGGACTTGGAAGGTG+AGG 0.516452 7_3:+58467871 None:intergenic
ATGCTGACACGGGCAAGGCA+TGG 0.518272 7_3:+58467484 None:intergenic
GTGTAGTTGGTGAAGTGTTG+CGG 0.522503 7_3:+58468290 None:intergenic
GGTGTTGATGCCAGGGGATG+AGG 0.522735 7_3:-58467522 Msa1112460:CDS
TCAATCTCTCTCTCATCTTG+AGG 0.522920 7_3:-58467915 Msa1112460:CDS
GACACATCTCGACCGTCCCT+CGG 0.527794 7_3:-58468034 Msa1112460:CDS
AACTTCGGTACCTCATCCCC+TGG 0.529530 7_3:+58467512 None:intergenic
CGCGAATGCTGACACGGGCA+AGG 0.530171 7_3:+58467479 None:intergenic
GAGATGGAAGGGGAAAAGAA+GGG 0.530732 7_3:-58467557 Msa1112460:CDS
AGATGGAAGGGGAAAAGAAG+GGG 0.531883 7_3:-58467556 Msa1112460:CDS
AGCTGAAGATGATGAGCAAG+AGG 0.534577 7_3:+58467990 None:intergenic
TATCAAAGGTAATATGATGA+TGG 0.538330 7_3:-58468086 Msa1112460:exon
GGTGATCACGGTGGTTTGGT+CGG 0.540559 7_3:+58467422 None:intergenic
TCTAGCCGCGAATGCTGACA+CGG 0.546125 7_3:+58467473 None:intergenic
CTTCTCATGTTGCTACCACT+GGG 0.549662 7_3:-58467957 Msa1112460:CDS
AAAAGACCAAAGGATTATGC+AGG 0.554133 7_3:-58467215 Msa1112460:three_prime_UTR
GGATATTTAAACACTTTAGA+GGG 0.560090 7_3:+58468331 None:intergenic
GATCACGGTGGTTTGGTCGG+TGG 0.567091 7_3:+58467425 None:intergenic
TTGGTGGATAGGAGATGGAA+GGG 0.567734 7_3:-58467568 Msa1112460:intron
GTTGGTGGATAGGAGATGGA+AGG 0.570134 7_3:-58467569 Msa1112460:intron
GGTCGAGATGTGTCTACTTG+AGG 0.570607 7_3:+58468043 None:intergenic
TTTATATGAAATAGAAAGGG+TGG 0.571866 7_3:+58468241 None:intergenic
GTGTTGCGGGAAAGGAAAGT+AGG 0.573190 7_3:+58468304 None:intergenic
ACATACTACAATGGTGATCA+CGG 0.583414 7_3:+58467410 None:intergenic
TAGAGAAAAGGAGCAAGTTG+AGG 0.583790 7_3:+58468363 None:intergenic
GAGGATTGAGTATGTGCTTG+TGG 0.589598 7_3:+58467890 None:intergenic
GAGATGTGTCTACTTGAGGT+GGG 0.592423 7_3:+58468047 None:intergenic
AAAGGATTATGCAGGTGGCA+AGG 0.602551 7_3:-58467207 Msa1112460:three_prime_UTR
TGATTTATATGAAATAGAAA+GGG 0.602838 7_3:+58468238 None:intergenic
TGGTGGATAGGAGATGGAAG+GGG 0.602910 7_3:-58467567 Msa1112460:intron
GCGGGAAAGGAAAGTAGGCG+TGG 0.603747 7_3:+58468309 None:intergenic
CATGTATTCTTCTATACACA+TGG 0.605759 7_3:+58467358 None:intergenic
AGAGGAGAGCAGTACCTAGT+GGG 0.605822 7_3:+58468008 None:intergenic
CTAGCCGCGAATGCTGACAC+GGG 0.608171 7_3:+58467474 None:intergenic
GTGTGACGGTGTTGATGCCA+GGG 0.609269 7_3:-58467529 Msa1112460:CDS
CAAGGCATGGCTATAAACTT+CGG 0.609997 7_3:+58467497 None:intergenic
CGGGAAAGGAAAGTAGGCGT+GGG 0.617775 7_3:+58468310 None:intergenic
GAGTGAGATTTGGACTTGGA+AGG 0.621951 7_3:+58467866 None:intergenic
AAGTTATAAGAGCTTATCAA+AGG 0.623107 7_3:-58468100 Msa1112460:five_prime_UTR
CAGTACCTAGTGGGAAACCG+AGG 0.623236 7_3:+58468017 None:intergenic
AGACCAAAGGATTATGCAGG+TGG 0.626537 7_3:-58467212 Msa1112460:three_prime_UTR
CAAGTCCAAATCTCACTCCA+CGG 0.634220 7_3:-58467861 Msa1112460:intron
AAAGAAGGGGCAGAGTGTGA+CGG 0.635702 7_3:-58467543 Msa1112460:CDS
AGATGTGTCTACTTGAGGTG+GGG 0.637020 7_3:+58468048 None:intergenic
AAGAGGAGAGCAGTACCTAG+TGG 0.638253 7_3:+58468007 None:intergenic
GTGCCTACACACGAGACAGT+AGG 0.642189 7_3:+58467137 None:intergenic
GTCGCTGAGGAAATTAAGGA+AGG 0.643661 7_3:+58468193 None:intergenic
CGATAATTAACATACTACAA+TGG 0.657602 7_3:+58467401 None:intergenic
AGTACCTAGTGGGAAACCGA+GGG 0.682252 7_3:+58468018 None:intergenic
CCTAGTGGGAAACCGAGGGA+CGG 0.686417 7_3:+58468022 None:intergenic
TATGGATGTGTGTGTCGCTG+AGG 0.711993 7_3:+58468180 None:intergenic
TGTGACGGTGTTGATGCCAG+GGG 0.749010 7_3:-58467528 Msa1112460:CDS
TACTACAATGGTGATCACGG+TGG 0.756673 7_3:+58467413 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGATTTATATGAAATAGAAA+GGG + chr7_3:58467255-58467274 None:intergenic 15.0%
!! ATGATTTATATGAAATAGAA+AGG + chr7_3:58467256-58467275 None:intergenic 15.0%
!!! AATTGATTATAATTTTCTCT+TGG + chr7_3:58467769-58467788 None:intergenic 15.0%
!!! AATTTTACAATTTTGTTTGT+TGG - chr7_3:58467903-58467922 Msa1112460:CDS 15.0%
!!! TTATAATATATCTTTTTCGT+CGG + chr7_3:58468256-58468275 None:intergenic 15.0%
!! ATATATTATAAAAAGACCAA+AGG - chr7_3:58468265-58468284 Msa1112460:five_prime_UTR 15.0%
!! TGATTTATATGAAATAGAAA+GGG + chr7_3:58467255-58467274 None:intergenic 15.0%
!! ATGATTTATATGAAATAGAA+AGG + chr7_3:58467256-58467275 None:intergenic 15.0%
!!! AATTGATTATAATTTTCTCT+TGG + chr7_3:58467769-58467788 None:intergenic 15.0%
!!! AATTTTACAATTTTGTTTGT+TGG - chr7_3:58467903-58467922 Msa1112460:CDS 15.0%
!!! TTATAATATATCTTTTTCGT+CGG + chr7_3:58468256-58468275 None:intergenic 15.0%
!! ATATATTATAAAAAGACCAA+AGG - chr7_3:58468265-58468284 Msa1112460:five_prime_UTR 15.0%
!! AAGGATTAACTATAACTTAT+GGG + chr7_3:58467281-58467300 None:intergenic 20.0%
!! AAAATACTACTACTCATATA+TGG + chr7_3:58467331-58467350 None:intergenic 20.0%
!!! TATAATATATCTTTTTCGTC+GGG + chr7_3:58468255-58468274 None:intergenic 20.0%
!! AAGGATTAACTATAACTTAT+GGG + chr7_3:58467281-58467300 None:intergenic 20.0%
!! AAAATACTACTACTCATATA+TGG + chr7_3:58467331-58467350 None:intergenic 20.0%
!!! TATAATATATCTTTTTCGTC+GGG + chr7_3:58468255-58468274 None:intergenic 20.0%
! GGATATTTAAACACTTTAGA+GGG + chr7_3:58467162-58467181 None:intergenic 25.0%
! TTTATATGAAATAGAAAGGG+TGG + chr7_3:58467252-58467271 None:intergenic 25.0%
! GAAGGATTAACTATAACTTA+TGG + chr7_3:58467282-58467301 None:intergenic 25.0%
! AAGTTATAAGAGCTTATCAA+AGG - chr7_3:58467390-58467409 Msa1112460:three_prime_UTR 25.0%
! TATCAAAGGTAATATGATGA+TGG - chr7_3:58467404-58467423 Msa1112460:three_prime_UTR 25.0%
!!! TTTACAATTTTGTTTGTTGG+TGG - chr7_3:58467906-58467925 Msa1112460:CDS 25.0%
! CGATAATTAACATACTACAA+TGG + chr7_3:58468092-58468111 None:intergenic 25.0%
! GGATATTTAAACACTTTAGA+GGG + chr7_3:58467162-58467181 None:intergenic 25.0%
! TTTATATGAAATAGAAAGGG+TGG + chr7_3:58467252-58467271 None:intergenic 25.0%
! GAAGGATTAACTATAACTTA+TGG + chr7_3:58467282-58467301 None:intergenic 25.0%
! AAGTTATAAGAGCTTATCAA+AGG - chr7_3:58467390-58467409 Msa1112460:three_prime_UTR 25.0%
! TATCAAAGGTAATATGATGA+TGG - chr7_3:58467404-58467423 Msa1112460:three_prime_UTR 25.0%
!!! TTTACAATTTTGTTTGTTGG+TGG - chr7_3:58467906-58467925 Msa1112460:CDS 25.0%
! CGATAATTAACATACTACAA+TGG + chr7_3:58468092-58468111 None:intergenic 25.0%
GGAAAGTAAAGAAACTTTCA+TGG + chr7_3:58467109-58467128 None:intergenic 30.0%
GGGATATTTAAACACTTTAG+AGG + chr7_3:58467163-58467182 None:intergenic 30.0%
AAAGAAAAGGATTGTGTAGT+TGG + chr7_3:58467216-58467235 None:intergenic 30.0%
AAAGAGTGAAATTATTACCG+TGG + chr7_3:58467649-58467668 None:intergenic 30.0%
AGGATCATACAGTTGTAAAA+TGG + chr7_3:58467808-58467827 None:intergenic 30.0%
TTGACGTTGTAAGTCAAAAA+AGG + chr7_3:58467828-58467847 None:intergenic 30.0%
!! AATTTTGTTTGTTGGTGGAT+AGG - chr7_3:58467911-58467930 Msa1112460:CDS 30.0%
CATGTATTCTTCTATACACA+TGG + chr7_3:58468135-58468154 None:intergenic 30.0%
GGAAAGTAAAGAAACTTTCA+TGG + chr7_3:58467109-58467128 None:intergenic 30.0%
GGGATATTTAAACACTTTAG+AGG + chr7_3:58467163-58467182 None:intergenic 30.0%
AAAGAAAAGGATTGTGTAGT+TGG + chr7_3:58467216-58467235 None:intergenic 30.0%
AAAGAGTGAAATTATTACCG+TGG + chr7_3:58467649-58467668 None:intergenic 30.0%
AGGATCATACAGTTGTAAAA+TGG + chr7_3:58467808-58467827 None:intergenic 30.0%
TTGACGTTGTAAGTCAAAAA+AGG + chr7_3:58467828-58467847 None:intergenic 30.0%
!! AATTTTGTTTGTTGGTGGAT+AGG - chr7_3:58467911-58467930 Msa1112460:CDS 30.0%
CATGTATTCTTCTATACACA+TGG + chr7_3:58468135-58468154 None:intergenic 30.0%
CAAGCAAAATGGGAAAGAAA+AGG + chr7_3:58467229-58467248 None:intergenic 35.0%
ACATACTACAATGGTGATCA+CGG + chr7_3:58468083-58468102 None:intergenic 35.0%
AAAAGACCAAAGGATTATGC+AGG - chr7_3:58468275-58468294 Msa1112460:five_prime_UTR 35.0%
CGAACTGTATTTGTAGTTTG+CGG - chr7_3:58468386-58468405 Msa1112460:five_prime_UTR 35.0%
CAAGCAAAATGGGAAAGAAA+AGG + chr7_3:58467229-58467248 None:intergenic 35.0%
ACATACTACAATGGTGATCA+CGG + chr7_3:58468083-58468102 None:intergenic 35.0%
AAAAGACCAAAGGATTATGC+AGG - chr7_3:58468275-58468294 Msa1112460:five_prime_UTR 35.0%
CGAACTGTATTTGTAGTTTG+CGG - chr7_3:58468386-58468405 Msa1112460:five_prime_UTR 35.0%
TAGAGAAAAGGAGCAAGTTG+AGG + chr7_3:58467130-58467149 None:intergenic 40.0%
GGGTGTATCAACTAGAGAAA+AGG + chr7_3:58467142-58467161 None:intergenic 40.0%
CTCATCATCTTCAGCTTAAG+TGG - chr7_3:58467507-58467526 Msa1112460:CDS 40.0%
TCAATCTCTCTCTCATCTTG+AGG - chr7_3:58467575-58467594 Msa1112460:intron 40.0%
TATTACCGTGGAGTGAGATT+TGG + chr7_3:58467637-58467656 None:intergenic 40.0%
CAAGGCATGGCTATAAACTT+CGG + chr7_3:58467996-58468015 None:intergenic 40.0%
TAGAGAAAAGGAGCAAGTTG+AGG + chr7_3:58467130-58467149 None:intergenic 40.0%
GGGTGTATCAACTAGAGAAA+AGG + chr7_3:58467142-58467161 None:intergenic 40.0%
CTCATCATCTTCAGCTTAAG+TGG - chr7_3:58467507-58467526 Msa1112460:CDS 40.0%
TCAATCTCTCTCTCATCTTG+AGG - chr7_3:58467575-58467594 Msa1112460:intron 40.0%
TATTACCGTGGAGTGAGATT+TGG + chr7_3:58467637-58467656 None:intergenic 40.0%
CAAGGCATGGCTATAAACTT+CGG + chr7_3:58467996-58468015 None:intergenic 40.0%
!! TGTAGTTGGTGAAGTGTTGC+GGG + chr7_3:58467202-58467221 None:intergenic 45.0%
!! GTGTAGTTGGTGAAGTGTTG+CGG + chr7_3:58467203-58467222 None:intergenic 45.0%
GAAAGGGTGGCAAGCAAAAT+GGG + chr7_3:58467239-58467258 None:intergenic 45.0%
AGAAAGGGTGGCAAGCAAAA+TGG + chr7_3:58467240-58467259 None:intergenic 45.0%
GTCGCTGAGGAAATTAAGGA+AGG + chr7_3:58467300-58467319 None:intergenic 45.0%
! GTGTGTCGCTGAGGAAATTA+AGG + chr7_3:58467304-58467323 None:intergenic 45.0%
!!! TGTTTTGATGCTGATGCTGG+TGG + chr7_3:58467367-58467386 None:intergenic 45.0%
!!! GCTTGTTTTGATGCTGATGC+TGG + chr7_3:58467370-58467389 None:intergenic 45.0%
AGATGTGTCTACTTGAGGTG+GGG + chr7_3:58467445-58467464 None:intergenic 45.0%
GAGATGTGTCTACTTGAGGT+GGG + chr7_3:58467446-58467465 None:intergenic 45.0%
AGCTGAAGATGATGAGCAAG+AGG + chr7_3:58467503-58467522 None:intergenic 45.0%
TCTTCTCATGTTGCTACCAC+TGG - chr7_3:58467532-58467551 Msa1112460:CDS 45.0%
CTTCTCATGTTGCTACCACT+GGG - chr7_3:58467533-58467552 Msa1112460:CDS 45.0%
! GTGAACGAAACTTTTCCCAG+TGG + chr7_3:58467551-58467570 None:intergenic 45.0%
GAGGATTGAGTATGTGCTTG+TGG + chr7_3:58467603-58467622 None:intergenic 45.0%
AGATTTGGACTTGGAAGGTG+AGG + chr7_3:58467622-58467641 None:intergenic 45.0%
GAGTGAGATTTGGACTTGGA+AGG + chr7_3:58467627-58467646 None:intergenic 45.0%
CAAGTCCAAATCTCACTCCA+CGG - chr7_3:58467629-58467648 Msa1112460:intron 45.0%
! GTTTGTTGGTGGATAGGAGA+TGG - chr7_3:58467917-58467936 Msa1112460:CDS 45.0%
! TTGGTGGATAGGAGATGGAA+GGG - chr7_3:58467922-58467941 Msa1112460:CDS 45.0%
GAGATGGAAGGGGAAAAGAA+GGG - chr7_3:58467933-58467952 Msa1112460:CDS 45.0%
AGATGGAAGGGGAAAAGAAG+GGG - chr7_3:58467934-58467953 Msa1112460:CDS 45.0%
TACTACAATGGTGATCACGG+TGG + chr7_3:58468080-58468099 None:intergenic 45.0%
TTCGTCGGGACAAATGTGTT+TGG + chr7_3:58468241-58468260 None:intergenic 45.0%
AGACCAAAGGATTATGCAGG+TGG - chr7_3:58468278-58468297 Msa1112460:five_prime_UTR 45.0%
TTGCCACCTGCATAATCCTT+TGG + chr7_3:58468284-58468303 None:intergenic 45.0%
AAAGGATTATGCAGGTGGCA+AGG - chr7_3:58468283-58468302 Msa1112460:five_prime_UTR 45.0%
!! TGTAGTTGGTGAAGTGTTGC+GGG + chr7_3:58467202-58467221 None:intergenic 45.0%
!! GTGTAGTTGGTGAAGTGTTG+CGG + chr7_3:58467203-58467222 None:intergenic 45.0%
GAAAGGGTGGCAAGCAAAAT+GGG + chr7_3:58467239-58467258 None:intergenic 45.0%
AGAAAGGGTGGCAAGCAAAA+TGG + chr7_3:58467240-58467259 None:intergenic 45.0%
GTCGCTGAGGAAATTAAGGA+AGG + chr7_3:58467300-58467319 None:intergenic 45.0%
! GTGTGTCGCTGAGGAAATTA+AGG + chr7_3:58467304-58467323 None:intergenic 45.0%
!!! TGTTTTGATGCTGATGCTGG+TGG + chr7_3:58467367-58467386 None:intergenic 45.0%
!!! GCTTGTTTTGATGCTGATGC+TGG + chr7_3:58467370-58467389 None:intergenic 45.0%
AGATGTGTCTACTTGAGGTG+GGG + chr7_3:58467445-58467464 None:intergenic 45.0%
GAGATGTGTCTACTTGAGGT+GGG + chr7_3:58467446-58467465 None:intergenic 45.0%
AGCTGAAGATGATGAGCAAG+AGG + chr7_3:58467503-58467522 None:intergenic 45.0%
TCTTCTCATGTTGCTACCAC+TGG - chr7_3:58467532-58467551 Msa1112460:CDS 45.0%
CTTCTCATGTTGCTACCACT+GGG - chr7_3:58467533-58467552 Msa1112460:CDS 45.0%
! GTGAACGAAACTTTTCCCAG+TGG + chr7_3:58467551-58467570 None:intergenic 45.0%
GAGGATTGAGTATGTGCTTG+TGG + chr7_3:58467603-58467622 None:intergenic 45.0%
AGATTTGGACTTGGAAGGTG+AGG + chr7_3:58467622-58467641 None:intergenic 45.0%
GAGTGAGATTTGGACTTGGA+AGG + chr7_3:58467627-58467646 None:intergenic 45.0%
CAAGTCCAAATCTCACTCCA+CGG - chr7_3:58467629-58467648 Msa1112460:intron 45.0%
! GTTTGTTGGTGGATAGGAGA+TGG - chr7_3:58467917-58467936 Msa1112460:CDS 45.0%
! TTGGTGGATAGGAGATGGAA+GGG - chr7_3:58467922-58467941 Msa1112460:CDS 45.0%
GAGATGGAAGGGGAAAAGAA+GGG - chr7_3:58467933-58467952 Msa1112460:CDS 45.0%
AGATGGAAGGGGAAAAGAAG+GGG - chr7_3:58467934-58467953 Msa1112460:CDS 45.0%
TACTACAATGGTGATCACGG+TGG + chr7_3:58468080-58468099 None:intergenic 45.0%
TTCGTCGGGACAAATGTGTT+TGG + chr7_3:58468241-58468260 None:intergenic 45.0%
AGACCAAAGGATTATGCAGG+TGG - chr7_3:58468278-58468297 Msa1112460:five_prime_UTR 45.0%
TTGCCACCTGCATAATCCTT+TGG + chr7_3:58468284-58468303 None:intergenic 45.0%
AAAGGATTATGCAGGTGGCA+AGG - chr7_3:58468283-58468302 Msa1112460:five_prime_UTR 45.0%
!!! ATAATTTGAAATTATAAAAT+TGG - chr7_3:58467711-58467730 Msa1112460:intron 5.0%
!!! ATAATTTGAAATTATAAAAT+TGG - chr7_3:58467711-58467730 Msa1112460:intron 5.0%
GTGTTGCGGGAAAGGAAAGT+AGG + chr7_3:58467189-58467208 None:intergenic 50.0%
!! TTGGTGAAGTGTTGCGGGAA+AGG + chr7_3:58467197-58467216 None:intergenic 50.0%
! TATGGATGTGTGTGTCGCTG+AGG + chr7_3:58467313-58467332 None:intergenic 50.0%
CTTGAGGTGGGGAAAGATCT+GGG + chr7_3:58467434-58467453 None:intergenic 50.0%
ACTTGAGGTGGGGAAAGATC+TGG + chr7_3:58467435-58467454 None:intergenic 50.0%
CGAGATGTGTCTACTTGAGG+TGG + chr7_3:58467447-58467466 None:intergenic 50.0%
GGTCGAGATGTGTCTACTTG+AGG + chr7_3:58467450-58467469 None:intergenic 50.0%
AGTACCTAGTGGGAAACCGA+GGG + chr7_3:58467475-58467494 None:intergenic 50.0%
AGAGGAGAGCAGTACCTAGT+GGG + chr7_3:58467485-58467504 None:intergenic 50.0%
AAGAGGAGAGCAGTACCTAG+TGG + chr7_3:58467486-58467505 None:intergenic 50.0%
CGTGGAGTGAGATTTGGACT+TGG + chr7_3:58467631-58467650 None:intergenic 50.0%
! GTTGGTGGATAGGAGATGGA+AGG - chr7_3:58467921-58467940 Msa1112460:CDS 50.0%
! TGGTGGATAGGAGATGGAAG+GGG - chr7_3:58467923-58467942 Msa1112460:CDS 50.0%
GGAGATGGAAGGGGAAAAGA+AGG - chr7_3:58467932-58467951 Msa1112460:CDS 50.0%
AAAGAAGGGGCAGAGTGTGA+CGG - chr7_3:58467947-58467966 Msa1112460:CDS 50.0%
CAATGGTGATCACGGTGGTT+TGG + chr7_3:58468075-58468094 None:intergenic 50.0%
CTTCCTACTGTCTCGTGTGT+AGG - chr7_3:58468350-58468369 Msa1112460:five_prime_UTR 50.0%
GTGTTGCGGGAAAGGAAAGT+AGG + chr7_3:58467189-58467208 None:intergenic 50.0%
!! TTGGTGAAGTGTTGCGGGAA+AGG + chr7_3:58467197-58467216 None:intergenic 50.0%
! TATGGATGTGTGTGTCGCTG+AGG + chr7_3:58467313-58467332 None:intergenic 50.0%
CTTGAGGTGGGGAAAGATCT+GGG + chr7_3:58467434-58467453 None:intergenic 50.0%
ACTTGAGGTGGGGAAAGATC+TGG + chr7_3:58467435-58467454 None:intergenic 50.0%
CGAGATGTGTCTACTTGAGG+TGG + chr7_3:58467447-58467466 None:intergenic 50.0%
GGTCGAGATGTGTCTACTTG+AGG + chr7_3:58467450-58467469 None:intergenic 50.0%
AGTACCTAGTGGGAAACCGA+GGG + chr7_3:58467475-58467494 None:intergenic 50.0%
AGAGGAGAGCAGTACCTAGT+GGG + chr7_3:58467485-58467504 None:intergenic 50.0%
AAGAGGAGAGCAGTACCTAG+TGG + chr7_3:58467486-58467505 None:intergenic 50.0%
CGTGGAGTGAGATTTGGACT+TGG + chr7_3:58467631-58467650 None:intergenic 50.0%
! GTTGGTGGATAGGAGATGGA+AGG - chr7_3:58467921-58467940 Msa1112460:CDS 50.0%
! TGGTGGATAGGAGATGGAAG+GGG - chr7_3:58467923-58467942 Msa1112460:CDS 50.0%
GGAGATGGAAGGGGAAAAGA+AGG - chr7_3:58467932-58467951 Msa1112460:CDS 50.0%
AAAGAAGGGGCAGAGTGTGA+CGG - chr7_3:58467947-58467966 Msa1112460:CDS 50.0%
CAATGGTGATCACGGTGGTT+TGG + chr7_3:58468075-58468094 None:intergenic 50.0%
CTTCCTACTGTCTCGTGTGT+AGG - chr7_3:58468350-58468369 Msa1112460:five_prime_UTR 50.0%
CGGGAAAGGAAAGTAGGCGT+GGG + chr7_3:58467183-58467202 None:intergenic 55.0%
CAGTACCTAGTGGGAAACCG+AGG + chr7_3:58467476-58467495 None:intergenic 55.0%
!! AGTGTGACGGTGTTGATGCC+AGG - chr7_3:58467960-58467979 Msa1112460:CDS 55.0%
!! GTGTGACGGTGTTGATGCCA+GGG - chr7_3:58467961-58467980 Msa1112460:CDS 55.0%
!! TGTGACGGTGTTGATGCCAG+GGG - chr7_3:58467962-58467981 Msa1112460:CDS 55.0%
AACTTCGGTACCTCATCCCC+TGG + chr7_3:58467981-58468000 None:intergenic 55.0%
TCTAGCCGCGAATGCTGACA+CGG + chr7_3:58468020-58468039 None:intergenic 55.0%
!! GGTGATCACGGTGGTTTGGT+CGG + chr7_3:58468071-58468090 None:intergenic 55.0%
GTGCCTACACACGAGACAGT+AGG + chr7_3:58468356-58468375 None:intergenic 55.0%
TGCGGAGATGCAACTACTGC+TGG - chr7_3:58468404-58468423 Msa1112460:five_prime_UTR 55.0%
CGGGAAAGGAAAGTAGGCGT+GGG + chr7_3:58467183-58467202 None:intergenic 55.0%
CAGTACCTAGTGGGAAACCG+AGG + chr7_3:58467476-58467495 None:intergenic 55.0%
!! AGTGTGACGGTGTTGATGCC+AGG - chr7_3:58467960-58467979 Msa1112460:CDS 55.0%
!! GTGTGACGGTGTTGATGCCA+GGG - chr7_3:58467961-58467980 Msa1112460:CDS 55.0%
!! TGTGACGGTGTTGATGCCAG+GGG - chr7_3:58467962-58467981 Msa1112460:CDS 55.0%
AACTTCGGTACCTCATCCCC+TGG + chr7_3:58467981-58468000 None:intergenic 55.0%
TCTAGCCGCGAATGCTGACA+CGG + chr7_3:58468020-58468039 None:intergenic 55.0%
!! GGTGATCACGGTGGTTTGGT+CGG + chr7_3:58468071-58468090 None:intergenic 55.0%
GTGCCTACACACGAGACAGT+AGG + chr7_3:58468356-58468375 None:intergenic 55.0%
GCGGGAAAGGAAAGTAGGCG+TGG + chr7_3:58467184-58467203 None:intergenic 60.0%
GACACATCTCGACCGTCCCT+CGG - chr7_3:58467456-58467475 Msa1112460:CDS 60.0%
CCTAGTGGGAAACCGAGGGA+CGG + chr7_3:58467471-58467490 None:intergenic 60.0%
!! GGTGTTGATGCCAGGGGATG+AGG - chr7_3:58467968-58467987 Msa1112460:CDS 60.0%
ATGCTGACACGGGCAAGGCA+TGG + chr7_3:58468009-58468028 None:intergenic 60.0%
CTTGCCCGTGTCAGCATTCG+CGG - chr7_3:58468012-58468031 Msa1112460:CDS 60.0%
CTAGCCGCGAATGCTGACAC+GGG + chr7_3:58468019-58468038 None:intergenic 60.0%
!! GATCACGGTGGTTTGGTCGG+TGG + chr7_3:58468068-58468087 None:intergenic 60.0%
GCGGGAAAGGAAAGTAGGCG+TGG + chr7_3:58467184-58467203 None:intergenic 60.0%
GACACATCTCGACCGTCCCT+CGG - chr7_3:58467456-58467475 Msa1112460:CDS 60.0%
CCTAGTGGGAAACCGAGGGA+CGG + chr7_3:58467471-58467490 None:intergenic 60.0%
!! GGTGTTGATGCCAGGGGATG+AGG - chr7_3:58467968-58467987 Msa1112460:CDS 60.0%
ATGCTGACACGGGCAAGGCA+TGG + chr7_3:58468009-58468028 None:intergenic 60.0%
CTTGCCCGTGTCAGCATTCG+CGG - chr7_3:58468012-58468031 Msa1112460:CDS 60.0%
CTAGCCGCGAATGCTGACAC+GGG + chr7_3:58468019-58468038 None:intergenic 60.0%
!! GATCACGGTGGTTTGGTCGG+TGG + chr7_3:58468068-58468087 None:intergenic 60.0%
CCGTCCCTCGGTTTCCCACT+AGG - chr7_3:58467468-58467487 Msa1112460:CDS 65.0%
CGCGAATGCTGACACGGGCA+AGG + chr7_3:58468014-58468033 None:intergenic 65.0%
CCGTCCCTCGGTTTCCCACT+AGG - chr7_3:58467468-58467487 Msa1112460:CDS 65.0%
CGCGAATGCTGACACGGGCA+AGG + chr7_3:58468014-58468033 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr7_3 gene 58467088 58468424 58467088 ID=Msa1112460;Name=Msa1112460
chr7_3 mRNA 58467088 58468424 58467088 ID=Msa1112460-mRNA-1;Parent=Msa1112460;Name=Msa1112460-mRNA-1;_AED=0.00;_eAED=0.00;_QI=329|1|1|1|1|1|2|339|128
chr7_3 exon 58467088 58467579 58467088 ID=Msa1112460-mRNA-1:exon:12810;Parent=Msa1112460-mRNA-1
chr7_3 exon 58467862 58468424 58467862 ID=Msa1112460-mRNA-1:exon:12809;Parent=Msa1112460-mRNA-1
chr7_3 five_prime_UTR 58468096 58468424 58468096 ID=Msa1112460-mRNA-1:five_prime_utr;Parent=Msa1112460-mRNA-1
chr7_3 CDS 58467862 58468095 58467862 ID=Msa1112460-mRNA-1:cds;Parent=Msa1112460-mRNA-1
chr7_3 CDS 58467427 58467579 58467427 ID=Msa1112460-mRNA-1:cds;Parent=Msa1112460-mRNA-1
chr7_3 three_prime_UTR 58467088 58467426 58467088 ID=Msa1112460-mRNA-1:three_prime_utr;Parent=Msa1112460-mRNA-1
Gene Sequence

>Msa1112460

ATGATGATGGAGAGCCCAGATCTTTCCCCACCTCAAGTAGACACATCTCGACCGTCCCTCGGTTTCCCACTAGGTACTGCTCTCCTCTTGCTCATCATCTTCAGCTTAAGTGGTATCTTCTCATGTTGCTACCACTGGGAAAAGTTTCGTTCACTTCATCAATCTCTCTCTCATCTTGAGGCTCCACAAGCACATACTCAATCCTCACCTTCCAAGTCCAAATCTCACTCCACGGAGATGGAAGGGGAAAAGAAGGGGCAGAGTGTGACGGTGTTGATGCCAGGGGATGAGGTACCGAAGTTTATAGCCATGCCTTGCCCGTGTCAGCATTCGCGGCTAGAAGAGATTATTGTCAACATTGAGAAGCCACCGACCAAACCACCGTGA
Protein sequence

>Msa1112460

MMMESPDLSPPQVDTSRPSLGFPLGTALLLLIIFSLSGIFSCCYHWEKFRSLHQSLSHLEAPQAHTQSSPSKSKSHSTEMEGEKKGQSVTVLMPGDEVPKFIAMPCPCQHSRLEEIIVNIEKPPTKPP*