AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1155950

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1155950 MtrunA17_Chr5g0411511 92.969 128 9 0 1 128 2 129 8.76e-76 220
Msa1155950 MtrunA17_Chr8g0366891 66.116 121 35 4 3 122 1 116 9.33e-49 152
Msa1155950 MtrunA17_Chr7g0249111 80.597 67 2 1 1 67 1 56 2.09e-22 83.2
Msa1155950 MtrunA17_Chr5g0428121 36.522 115 68 2 16 126 9 122 1.12e-11 57.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1155950 AT5G65660.1 58.268 127 51 2 2 128 1 125 1.45e-45 144

Find 81 sgRNAs with CRISPR-Local

Find 199 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAAAGGGTGGCAAGCAAAA+TGG 0.271852 7_4:+43938507 None:intergenic
GAAGGATTAACTATAACTTA+TGG 0.283702 7_4:+43938465 None:intergenic
TATTACCGTGGAGTGAGATT+TGG 0.322108 7_4:+43938110 None:intergenic
TTGCCACCTGCATAATCCTT+TGG 0.358271 7_4:+43937463 None:intergenic
AAAATACTACTACTCATATA+TGG 0.365240 7_4:+43938416 None:intergenic
GGGATATTTAAACACTTTAG+AGG 0.376848 7_4:+43938584 None:intergenic
AAGGATTAACTATAACTTAT+GGG 0.387964 7_4:+43938466 None:intergenic
ACTTGAGGTGGGGAAAGATC+TGG 0.392706 7_4:+43938312 None:intergenic
CTCATCATCTTCAGCTTAAG+TGG 0.396808 7_4:-43938237 Msa1155950:CDS
TGTAGTTGGTGAAGTGTTGC+GGG 0.397253 7_4:+43938545 None:intergenic
CTTGCCCGTGTCAGCATTCG+CGG 0.412175 7_4:-43937732 Msa1155950:CDS
GGAAAGTAAAGAAACTTTCA+TGG 0.421053 7_4:+43938638 None:intergenic
GTGTGTCGCTGAGGAAATTA+AGG 0.425009 7_4:+43938443 None:intergenic
TTCGTCGGGACAAATGTGTT+TGG 0.425626 7_4:+43937506 None:intergenic
CGAACTGTATTTGTAGTTTG+CGG 0.425667 7_4:-43937358 Msa1155950:three_prime_UTR
GAAAGGGTGGCAAGCAAAAT+GGG 0.433378 7_4:+43938508 None:intergenic
TCTTCTCATGTTGCTACCAC+TGG 0.447898 7_4:-43938212 Msa1155950:CDS
AGTGTGACGGTGTTGATGCC+AGG 0.456740 7_4:-43937784 Msa1155950:CDS
ATGATTTATATGAAATAGAA+AGG 0.457860 7_4:+43938491 None:intergenic
TTGGTGAAGTGTTGCGGGAA+AGG 0.458988 7_4:+43938550 None:intergenic
CAATGGTGATCACGGTGGTT+TGG 0.473390 7_4:+43937672 None:intergenic
GGGTGTATCAACTAGAGAAA+AGG 0.480576 7_4:+43938605 None:intergenic
CCGTCCCTCGGTTTCCCACT+AGG 0.483894 7_4:-43938276 Msa1155950:CDS
AACGAAAAGGATTGTGTAGT+TGG 0.502769 7_4:+43938531 None:intergenic
CGTGGAGTGAGATTTGGACT+TGG 0.508123 7_4:+43938116 None:intergenic
CTTGAGGTGGGGAAAGATCT+GGG 0.508977 7_4:+43938313 None:intergenic
CTTCCTACTGTCTCGTGTGT+AGG 0.509249 7_4:-43937394 Msa1155950:three_prime_UTR
CGAGATGTGTCTACTTGAGG+TGG 0.510749 7_4:+43938300 None:intergenic
AGATTTGGACTTGGAAGGTG+AGG 0.516452 7_4:+43938125 None:intergenic
ATGCTGACACGGGCAAGGCA+TGG 0.518272 7_4:+43937738 None:intergenic
GTGTAGTTGGTGAAGTGTTG+CGG 0.522503 7_4:+43938544 None:intergenic
GGTGTTGATGCCAGGGGATG+AGG 0.522735 7_4:-43937776 Msa1155950:CDS
TCAATCTCTCTCTCATCTTG+AGG 0.522920 7_4:-43938169 Msa1155950:CDS
GACACATCTCGACCGTCCCT+CGG 0.527794 7_4:-43938288 Msa1155950:CDS
AACTTCGGTACCTCATCCCC+TGG 0.529530 7_4:+43937766 None:intergenic
CGCGAATGCTGACACGGGCA+AGG 0.530171 7_4:+43937733 None:intergenic
AGCTGAAGATGATGAGCAAG+AGG 0.534577 7_4:+43938244 None:intergenic
TGGTGGATAGGAGATGGAAG+GGG 0.537876 7_4:-43937821 Msa1155950:intron
TATCAAAGGTAATATGATGA+TGG 0.538330 7_4:-43938340 Msa1155950:exon
CAAGCAAAATGGGAACGAAA+AGG 0.539062 7_4:+43938518 None:intergenic
GGTGATCACGGTGGTTTGGT+CGG 0.540559 7_4:+43937676 None:intergenic
TCTAGCCGCGAATGCTGACA+CGG 0.546125 7_4:+43937727 None:intergenic
CTTCTCATGTTGCTACCACT+GGG 0.549662 7_4:-43938211 Msa1155950:CDS
TTGGTGGATAGGAGATGGAA+GGG 0.551011 7_4:-43937822 Msa1155950:intron
AAAAGACCAAAGGATTATGC+AGG 0.554133 7_4:-43937469 Msa1155950:three_prime_UTR
GGTGGATAGGAGATGGAAGG+GGG 0.554283 7_4:-43937820 Msa1155950:intron
GGATATTTAAACACTTTAGA+GGG 0.560090 7_4:+43938585 None:intergenic
GTTGGTGGATAGGAGATGGA+AGG 0.562698 7_4:-43937823 Msa1155950:intron
GATCACGGTGGTTTGGTCGG+TGG 0.567091 7_4:+43937679 None:intergenic
GGTCGAGATGTGTCTACTTG+AGG 0.570607 7_4:+43938297 None:intergenic
TTTATATGAAATAGAAAGGG+TGG 0.571866 7_4:+43938495 None:intergenic
GTGTTGCGGGAAAGGAAAGT+AGG 0.573190 7_4:+43938558 None:intergenic
ACATACTACAATGGTGATCA+CGG 0.583414 7_4:+43937664 None:intergenic
TAGAGAAAAGGAGCAAGTTG+AGG 0.583790 7_4:+43938617 None:intergenic
GAGGATTGAGTATGTGCTTG+TGG 0.589598 7_4:+43938144 None:intergenic
GAGATGTGTCTACTTGAGGT+GGG 0.592423 7_4:+43938301 None:intergenic
AAAGAAGGGGCAGAGTGTGA+CGG 0.593264 7_4:-43937797 Msa1155950:CDS
AAAGGATTATGCAGGTGGCA+AGG 0.602551 7_4:-43937461 Msa1155950:three_prime_UTR
TGATTTATATGAAATAGAAA+GGG 0.602838 7_4:+43938492 None:intergenic
GCGGGAAAGGAAAGTAGGCG+TGG 0.603747 7_4:+43938563 None:intergenic
CATGTATTCTTCTATACACA+TGG 0.605759 7_4:+43937612 None:intergenic
AGAGGAGAGCAGTACCTAGT+GGG 0.605822 7_4:+43938262 None:intergenic
CTAGCCGCGAATGCTGACAC+GGG 0.608171 7_4:+43937728 None:intergenic
GTGTGACGGTGTTGATGCCA+GGG 0.609269 7_4:-43937783 Msa1155950:CDS
CAAGGCATGGCTATAAACTT+CGG 0.609997 7_4:+43937751 None:intergenic
CGGGAAAGGAAAGTAGGCGT+GGG 0.617775 7_4:+43938564 None:intergenic
GAGTGAGATTTGGACTTGGA+AGG 0.621951 7_4:+43938120 None:intergenic
AAGTTATAAGAGCTTATCAA+AGG 0.623107 7_4:-43938354 Msa1155950:five_prime_UTR
CAGTACCTAGTGGGAAACCG+AGG 0.623236 7_4:+43938271 None:intergenic
AGACCAAAGGATTATGCAGG+TGG 0.626537 7_4:-43937466 Msa1155950:three_prime_UTR
CAAGTCCAAATCTCACTCCA+CGG 0.634220 7_4:-43938115 Msa1155950:intron
AGATGTGTCTACTTGAGGTG+GGG 0.637020 7_4:+43938302 None:intergenic
AAGAGGAGAGCAGTACCTAG+TGG 0.638253 7_4:+43938261 None:intergenic
GTGCCTACACACGAGACAGT+AGG 0.642189 7_4:+43937391 None:intergenic
GTCGCTGAGGAAATTAAGGA+AGG 0.643661 7_4:+43938447 None:intergenic
CGATAATTAACATACTACAA+TGG 0.657602 7_4:+43937655 None:intergenic
AGTACCTAGTGGGAAACCGA+GGG 0.682252 7_4:+43938272 None:intergenic
CCTAGTGGGAAACCGAGGGA+CGG 0.686417 7_4:+43938276 None:intergenic
TATGGATGTGTGTGTCGCTG+AGG 0.711993 7_4:+43938434 None:intergenic
TGTGACGGTGTTGATGCCAG+GGG 0.749010 7_4:-43937782 Msa1155950:CDS
TACTACAATGGTGATCACGG+TGG 0.756673 7_4:+43937667 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGATTTATATGAAATAGAAA+GGG + chr7_4:43937509-43937528 None:intergenic 15.0%
!! ATGATTTATATGAAATAGAA+AGG + chr7_4:43937510-43937529 None:intergenic 15.0%
!!! AATTGATTATAATTTTCTCT+TGG + chr7_4:43938023-43938042 None:intergenic 15.0%
!!! AATTTTACAATTTTGTTTGT+TGG - chr7_4:43938157-43938176 Msa1155950:CDS 15.0%
!!! TTATAATATATCTTTTTCGT+CGG + chr7_4:43938510-43938529 None:intergenic 15.0%
!! ATATATTATAAAAAGACCAA+AGG - chr7_4:43938519-43938538 Msa1155950:five_prime_UTR 15.0%
!! TGATTTATATGAAATAGAAA+GGG + chr7_4:43937509-43937528 None:intergenic 15.0%
!! ATGATTTATATGAAATAGAA+AGG + chr7_4:43937510-43937529 None:intergenic 15.0%
!!! AATTGATTATAATTTTCTCT+TGG + chr7_4:43938023-43938042 None:intergenic 15.0%
!!! AATTTTACAATTTTGTTTGT+TGG - chr7_4:43938157-43938176 Msa1155950:CDS 15.0%
!!! TTATAATATATCTTTTTCGT+CGG + chr7_4:43938510-43938529 None:intergenic 15.0%
!! ATATATTATAAAAAGACCAA+AGG - chr7_4:43938519-43938538 Msa1155950:five_prime_UTR 15.0%
!! AAGGATTAACTATAACTTAT+GGG + chr7_4:43937535-43937554 None:intergenic 20.0%
!! AAAATACTACTACTCATATA+TGG + chr7_4:43937585-43937604 None:intergenic 20.0%
!!! TATAATATATCTTTTTCGTC+GGG + chr7_4:43938509-43938528 None:intergenic 20.0%
!! AAGGATTAACTATAACTTAT+GGG + chr7_4:43937535-43937554 None:intergenic 20.0%
!! AAAATACTACTACTCATATA+TGG + chr7_4:43937585-43937604 None:intergenic 20.0%
!!! TATAATATATCTTTTTCGTC+GGG + chr7_4:43938509-43938528 None:intergenic 20.0%
! GGATATTTAAACACTTTAGA+GGG + chr7_4:43937416-43937435 None:intergenic 25.0%
! TTTATATGAAATAGAAAGGG+TGG + chr7_4:43937506-43937525 None:intergenic 25.0%
! GAAGGATTAACTATAACTTA+TGG + chr7_4:43937536-43937555 None:intergenic 25.0%
! AAGTTATAAGAGCTTATCAA+AGG - chr7_4:43937644-43937663 Msa1155950:three_prime_UTR 25.0%
! TATCAAAGGTAATATGATGA+TGG - chr7_4:43937658-43937677 Msa1155950:three_prime_UTR 25.0%
!!! TTTACAATTTTGTTTGTTGG+TGG - chr7_4:43938160-43938179 Msa1155950:CDS 25.0%
! CGATAATTAACATACTACAA+TGG + chr7_4:43938346-43938365 None:intergenic 25.0%
! GGATATTTAAACACTTTAGA+GGG + chr7_4:43937416-43937435 None:intergenic 25.0%
! TTTATATGAAATAGAAAGGG+TGG + chr7_4:43937506-43937525 None:intergenic 25.0%
! GAAGGATTAACTATAACTTA+TGG + chr7_4:43937536-43937555 None:intergenic 25.0%
! AAGTTATAAGAGCTTATCAA+AGG - chr7_4:43937644-43937663 Msa1155950:three_prime_UTR 25.0%
! TATCAAAGGTAATATGATGA+TGG - chr7_4:43937658-43937677 Msa1155950:three_prime_UTR 25.0%
!!! TTTACAATTTTGTTTGTTGG+TGG - chr7_4:43938160-43938179 Msa1155950:CDS 25.0%
! CGATAATTAACATACTACAA+TGG + chr7_4:43938346-43938365 None:intergenic 25.0%
GGAAAGTAAAGAAACTTTCA+TGG + chr7_4:43937363-43937382 None:intergenic 30.0%
GGGATATTTAAACACTTTAG+AGG + chr7_4:43937417-43937436 None:intergenic 30.0%
AAAGAGTGAAATTATTACCG+TGG + chr7_4:43937903-43937922 None:intergenic 30.0%
AGGATCATACAGTTGTAAAA+TGG + chr7_4:43938062-43938081 None:intergenic 30.0%
TTGACGTTGTAAGTCAAAAA+AGG + chr7_4:43938082-43938101 None:intergenic 30.0%
!! AATTTTGTTTGTTGGTGGAT+AGG - chr7_4:43938165-43938184 Msa1155950:CDS 30.0%
CATGTATTCTTCTATACACA+TGG + chr7_4:43938389-43938408 None:intergenic 30.0%
GGAAAGTAAAGAAACTTTCA+TGG + chr7_4:43937363-43937382 None:intergenic 30.0%
GGGATATTTAAACACTTTAG+AGG + chr7_4:43937417-43937436 None:intergenic 30.0%
AAAGAGTGAAATTATTACCG+TGG + chr7_4:43937903-43937922 None:intergenic 30.0%
AGGATCATACAGTTGTAAAA+TGG + chr7_4:43938062-43938081 None:intergenic 30.0%
TTGACGTTGTAAGTCAAAAA+AGG + chr7_4:43938082-43938101 None:intergenic 30.0%
!! AATTTTGTTTGTTGGTGGAT+AGG - chr7_4:43938165-43938184 Msa1155950:CDS 30.0%
CATGTATTCTTCTATACACA+TGG + chr7_4:43938389-43938408 None:intergenic 30.0%
AACGAAAAGGATTGTGTAGT+TGG + chr7_4:43937470-43937489 None:intergenic 35.0%
ACATACTACAATGGTGATCA+CGG + chr7_4:43938337-43938356 None:intergenic 35.0%
AAAAGACCAAAGGATTATGC+AGG - chr7_4:43938529-43938548 Msa1155950:five_prime_UTR 35.0%
CGAACTGTATTTGTAGTTTG+CGG - chr7_4:43938640-43938659 Msa1155950:five_prime_UTR 35.0%
AACGAAAAGGATTGTGTAGT+TGG + chr7_4:43937470-43937489 None:intergenic 35.0%
ACATACTACAATGGTGATCA+CGG + chr7_4:43938337-43938356 None:intergenic 35.0%
AAAAGACCAAAGGATTATGC+AGG - chr7_4:43938529-43938548 Msa1155950:five_prime_UTR 35.0%
CGAACTGTATTTGTAGTTTG+CGG - chr7_4:43938640-43938659 Msa1155950:five_prime_UTR 35.0%
TAGAGAAAAGGAGCAAGTTG+AGG + chr7_4:43937384-43937403 None:intergenic 40.0%
GGGTGTATCAACTAGAGAAA+AGG + chr7_4:43937396-43937415 None:intergenic 40.0%
CAAGCAAAATGGGAACGAAA+AGG + chr7_4:43937483-43937502 None:intergenic 40.0%
CTCATCATCTTCAGCTTAAG+TGG - chr7_4:43937761-43937780 Msa1155950:CDS 40.0%
TCAATCTCTCTCTCATCTTG+AGG - chr7_4:43937829-43937848 Msa1155950:intron 40.0%
TATTACCGTGGAGTGAGATT+TGG + chr7_4:43937891-43937910 None:intergenic 40.0%
CAAGGCATGGCTATAAACTT+CGG + chr7_4:43938250-43938269 None:intergenic 40.0%
TAGAGAAAAGGAGCAAGTTG+AGG + chr7_4:43937384-43937403 None:intergenic 40.0%
GGGTGTATCAACTAGAGAAA+AGG + chr7_4:43937396-43937415 None:intergenic 40.0%
CAAGCAAAATGGGAACGAAA+AGG + chr7_4:43937483-43937502 None:intergenic 40.0%
CTCATCATCTTCAGCTTAAG+TGG - chr7_4:43937761-43937780 Msa1155950:CDS 40.0%
TCAATCTCTCTCTCATCTTG+AGG - chr7_4:43937829-43937848 Msa1155950:intron 40.0%
TATTACCGTGGAGTGAGATT+TGG + chr7_4:43937891-43937910 None:intergenic 40.0%
CAAGGCATGGCTATAAACTT+CGG + chr7_4:43938250-43938269 None:intergenic 40.0%
!! TGTAGTTGGTGAAGTGTTGC+GGG + chr7_4:43937456-43937475 None:intergenic 45.0%
!! GTGTAGTTGGTGAAGTGTTG+CGG + chr7_4:43937457-43937476 None:intergenic 45.0%
GAAAGGGTGGCAAGCAAAAT+GGG + chr7_4:43937493-43937512 None:intergenic 45.0%
AGAAAGGGTGGCAAGCAAAA+TGG + chr7_4:43937494-43937513 None:intergenic 45.0%
GTCGCTGAGGAAATTAAGGA+AGG + chr7_4:43937554-43937573 None:intergenic 45.0%
! GTGTGTCGCTGAGGAAATTA+AGG + chr7_4:43937558-43937577 None:intergenic 45.0%
!!! TGTTTTGATGCTGATGCTGG+TGG + chr7_4:43937621-43937640 None:intergenic 45.0%
!!! GCTTGTTTTGATGCTGATGC+TGG + chr7_4:43937624-43937643 None:intergenic 45.0%
AGATGTGTCTACTTGAGGTG+GGG + chr7_4:43937699-43937718 None:intergenic 45.0%
GAGATGTGTCTACTTGAGGT+GGG + chr7_4:43937700-43937719 None:intergenic 45.0%
AGCTGAAGATGATGAGCAAG+AGG + chr7_4:43937757-43937776 None:intergenic 45.0%
TCTTCTCATGTTGCTACCAC+TGG - chr7_4:43937786-43937805 Msa1155950:CDS 45.0%
CTTCTCATGTTGCTACCACT+GGG - chr7_4:43937787-43937806 Msa1155950:CDS 45.0%
! GTGAACGAAACTTTTCCCAG+TGG + chr7_4:43937805-43937824 None:intergenic 45.0%
GAGGATTGAGTATGTGCTTG+TGG + chr7_4:43937857-43937876 None:intergenic 45.0%
AGATTTGGACTTGGAAGGTG+AGG + chr7_4:43937876-43937895 None:intergenic 45.0%
GAGTGAGATTTGGACTTGGA+AGG + chr7_4:43937881-43937900 None:intergenic 45.0%
CAAGTCCAAATCTCACTCCA+CGG - chr7_4:43937883-43937902 Msa1155950:intron 45.0%
! GTTTGTTGGTGGATAGGAGA+TGG - chr7_4:43938171-43938190 Msa1155950:CDS 45.0%
! TTGGTGGATAGGAGATGGAA+GGG - chr7_4:43938176-43938195 Msa1155950:CDS 45.0%
TACTACAATGGTGATCACGG+TGG + chr7_4:43938334-43938353 None:intergenic 45.0%
TTCGTCGGGACAAATGTGTT+TGG + chr7_4:43938495-43938514 None:intergenic 45.0%
AGACCAAAGGATTATGCAGG+TGG - chr7_4:43938532-43938551 Msa1155950:five_prime_UTR 45.0%
TTGCCACCTGCATAATCCTT+TGG + chr7_4:43938538-43938557 None:intergenic 45.0%
AAAGGATTATGCAGGTGGCA+AGG - chr7_4:43938537-43938556 Msa1155950:five_prime_UTR 45.0%
!! TGTAGTTGGTGAAGTGTTGC+GGG + chr7_4:43937456-43937475 None:intergenic 45.0%
!! GTGTAGTTGGTGAAGTGTTG+CGG + chr7_4:43937457-43937476 None:intergenic 45.0%
GAAAGGGTGGCAAGCAAAAT+GGG + chr7_4:43937493-43937512 None:intergenic 45.0%
AGAAAGGGTGGCAAGCAAAA+TGG + chr7_4:43937494-43937513 None:intergenic 45.0%
GTCGCTGAGGAAATTAAGGA+AGG + chr7_4:43937554-43937573 None:intergenic 45.0%
! GTGTGTCGCTGAGGAAATTA+AGG + chr7_4:43937558-43937577 None:intergenic 45.0%
!!! TGTTTTGATGCTGATGCTGG+TGG + chr7_4:43937621-43937640 None:intergenic 45.0%
!!! GCTTGTTTTGATGCTGATGC+TGG + chr7_4:43937624-43937643 None:intergenic 45.0%
AGATGTGTCTACTTGAGGTG+GGG + chr7_4:43937699-43937718 None:intergenic 45.0%
GAGATGTGTCTACTTGAGGT+GGG + chr7_4:43937700-43937719 None:intergenic 45.0%
AGCTGAAGATGATGAGCAAG+AGG + chr7_4:43937757-43937776 None:intergenic 45.0%
TCTTCTCATGTTGCTACCAC+TGG - chr7_4:43937786-43937805 Msa1155950:CDS 45.0%
CTTCTCATGTTGCTACCACT+GGG - chr7_4:43937787-43937806 Msa1155950:CDS 45.0%
! GTGAACGAAACTTTTCCCAG+TGG + chr7_4:43937805-43937824 None:intergenic 45.0%
GAGGATTGAGTATGTGCTTG+TGG + chr7_4:43937857-43937876 None:intergenic 45.0%
AGATTTGGACTTGGAAGGTG+AGG + chr7_4:43937876-43937895 None:intergenic 45.0%
GAGTGAGATTTGGACTTGGA+AGG + chr7_4:43937881-43937900 None:intergenic 45.0%
CAAGTCCAAATCTCACTCCA+CGG - chr7_4:43937883-43937902 Msa1155950:intron 45.0%
! GTTTGTTGGTGGATAGGAGA+TGG - chr7_4:43938171-43938190 Msa1155950:CDS 45.0%
! TTGGTGGATAGGAGATGGAA+GGG - chr7_4:43938176-43938195 Msa1155950:CDS 45.0%
TACTACAATGGTGATCACGG+TGG + chr7_4:43938334-43938353 None:intergenic 45.0%
TTCGTCGGGACAAATGTGTT+TGG + chr7_4:43938495-43938514 None:intergenic 45.0%
AGACCAAAGGATTATGCAGG+TGG - chr7_4:43938532-43938551 Msa1155950:five_prime_UTR 45.0%
TTGCCACCTGCATAATCCTT+TGG + chr7_4:43938538-43938557 None:intergenic 45.0%
AAAGGATTATGCAGGTGGCA+AGG - chr7_4:43938537-43938556 Msa1155950:five_prime_UTR 45.0%
!!! ATAATTTGAAATTATAAAAT+TGG - chr7_4:43937965-43937984 Msa1155950:intron 5.0%
!!! ATAATTTGAAATTATAAAAT+TGG - chr7_4:43937965-43937984 Msa1155950:intron 5.0%
GTGTTGCGGGAAAGGAAAGT+AGG + chr7_4:43937443-43937462 None:intergenic 50.0%
!! TTGGTGAAGTGTTGCGGGAA+AGG + chr7_4:43937451-43937470 None:intergenic 50.0%
! TATGGATGTGTGTGTCGCTG+AGG + chr7_4:43937567-43937586 None:intergenic 50.0%
CTTGAGGTGGGGAAAGATCT+GGG + chr7_4:43937688-43937707 None:intergenic 50.0%
ACTTGAGGTGGGGAAAGATC+TGG + chr7_4:43937689-43937708 None:intergenic 50.0%
CGAGATGTGTCTACTTGAGG+TGG + chr7_4:43937701-43937720 None:intergenic 50.0%
GGTCGAGATGTGTCTACTTG+AGG + chr7_4:43937704-43937723 None:intergenic 50.0%
AGTACCTAGTGGGAAACCGA+GGG + chr7_4:43937729-43937748 None:intergenic 50.0%
AGAGGAGAGCAGTACCTAGT+GGG + chr7_4:43937739-43937758 None:intergenic 50.0%
AAGAGGAGAGCAGTACCTAG+TGG + chr7_4:43937740-43937759 None:intergenic 50.0%
CGTGGAGTGAGATTTGGACT+TGG + chr7_4:43937885-43937904 None:intergenic 50.0%
! GTTGGTGGATAGGAGATGGA+AGG - chr7_4:43938175-43938194 Msa1155950:CDS 50.0%
! TGGTGGATAGGAGATGGAAG+GGG - chr7_4:43938177-43938196 Msa1155950:CDS 50.0%
GAGATGGAAGGGGGAAAGAA+GGG - chr7_4:43938187-43938206 Msa1155950:CDS 50.0%
AGATGGAAGGGGGAAAGAAG+GGG - chr7_4:43938188-43938207 Msa1155950:CDS 50.0%
AAAGAAGGGGCAGAGTGTGA+CGG - chr7_4:43938201-43938220 Msa1155950:CDS 50.0%
CAATGGTGATCACGGTGGTT+TGG + chr7_4:43938329-43938348 None:intergenic 50.0%
CTTCCTACTGTCTCGTGTGT+AGG - chr7_4:43938604-43938623 Msa1155950:five_prime_UTR 50.0%
GTGTTGCGGGAAAGGAAAGT+AGG + chr7_4:43937443-43937462 None:intergenic 50.0%
!! TTGGTGAAGTGTTGCGGGAA+AGG + chr7_4:43937451-43937470 None:intergenic 50.0%
! TATGGATGTGTGTGTCGCTG+AGG + chr7_4:43937567-43937586 None:intergenic 50.0%
CTTGAGGTGGGGAAAGATCT+GGG + chr7_4:43937688-43937707 None:intergenic 50.0%
ACTTGAGGTGGGGAAAGATC+TGG + chr7_4:43937689-43937708 None:intergenic 50.0%
CGAGATGTGTCTACTTGAGG+TGG + chr7_4:43937701-43937720 None:intergenic 50.0%
GGTCGAGATGTGTCTACTTG+AGG + chr7_4:43937704-43937723 None:intergenic 50.0%
AGTACCTAGTGGGAAACCGA+GGG + chr7_4:43937729-43937748 None:intergenic 50.0%
AGAGGAGAGCAGTACCTAGT+GGG + chr7_4:43937739-43937758 None:intergenic 50.0%
AAGAGGAGAGCAGTACCTAG+TGG + chr7_4:43937740-43937759 None:intergenic 50.0%
CGTGGAGTGAGATTTGGACT+TGG + chr7_4:43937885-43937904 None:intergenic 50.0%
! GTTGGTGGATAGGAGATGGA+AGG - chr7_4:43938175-43938194 Msa1155950:CDS 50.0%
! TGGTGGATAGGAGATGGAAG+GGG - chr7_4:43938177-43938196 Msa1155950:CDS 50.0%
GAGATGGAAGGGGGAAAGAA+GGG - chr7_4:43938187-43938206 Msa1155950:CDS 50.0%
AGATGGAAGGGGGAAAGAAG+GGG - chr7_4:43938188-43938207 Msa1155950:CDS 50.0%
AAAGAAGGGGCAGAGTGTGA+CGG - chr7_4:43938201-43938220 Msa1155950:CDS 50.0%
CAATGGTGATCACGGTGGTT+TGG + chr7_4:43938329-43938348 None:intergenic 50.0%
CTTCCTACTGTCTCGTGTGT+AGG - chr7_4:43938604-43938623 Msa1155950:five_prime_UTR 50.0%
CGGGAAAGGAAAGTAGGCGT+GGG + chr7_4:43937437-43937456 None:intergenic 55.0%
CAGTACCTAGTGGGAAACCG+AGG + chr7_4:43937730-43937749 None:intergenic 55.0%
GGTGGATAGGAGATGGAAGG+GGG - chr7_4:43938178-43938197 Msa1155950:CDS 55.0%
GGAGATGGAAGGGGGAAAGA+AGG - chr7_4:43938186-43938205 Msa1155950:CDS 55.0%
!! AGTGTGACGGTGTTGATGCC+AGG - chr7_4:43938214-43938233 Msa1155950:CDS 55.0%
!! GTGTGACGGTGTTGATGCCA+GGG - chr7_4:43938215-43938234 Msa1155950:CDS 55.0%
!! TGTGACGGTGTTGATGCCAG+GGG - chr7_4:43938216-43938235 Msa1155950:CDS 55.0%
AACTTCGGTACCTCATCCCC+TGG + chr7_4:43938235-43938254 None:intergenic 55.0%
TCTAGCCGCGAATGCTGACA+CGG + chr7_4:43938274-43938293 None:intergenic 55.0%
!! GGTGATCACGGTGGTTTGGT+CGG + chr7_4:43938325-43938344 None:intergenic 55.0%
GTGCCTACACACGAGACAGT+AGG + chr7_4:43938610-43938629 None:intergenic 55.0%
TGCGGAGATGCAACTACTGC+TGG - chr7_4:43938658-43938677 Msa1155950:five_prime_UTR 55.0%
CGGGAAAGGAAAGTAGGCGT+GGG + chr7_4:43937437-43937456 None:intergenic 55.0%
CAGTACCTAGTGGGAAACCG+AGG + chr7_4:43937730-43937749 None:intergenic 55.0%
GGTGGATAGGAGATGGAAGG+GGG - chr7_4:43938178-43938197 Msa1155950:CDS 55.0%
GGAGATGGAAGGGGGAAAGA+AGG - chr7_4:43938186-43938205 Msa1155950:CDS 55.0%
!! AGTGTGACGGTGTTGATGCC+AGG - chr7_4:43938214-43938233 Msa1155950:CDS 55.0%
!! GTGTGACGGTGTTGATGCCA+GGG - chr7_4:43938215-43938234 Msa1155950:CDS 55.0%
!! TGTGACGGTGTTGATGCCAG+GGG - chr7_4:43938216-43938235 Msa1155950:CDS 55.0%
AACTTCGGTACCTCATCCCC+TGG + chr7_4:43938235-43938254 None:intergenic 55.0%
TCTAGCCGCGAATGCTGACA+CGG + chr7_4:43938274-43938293 None:intergenic 55.0%
!! GGTGATCACGGTGGTTTGGT+CGG + chr7_4:43938325-43938344 None:intergenic 55.0%
GTGCCTACACACGAGACAGT+AGG + chr7_4:43938610-43938629 None:intergenic 55.0%
GCGGGAAAGGAAAGTAGGCG+TGG + chr7_4:43937438-43937457 None:intergenic 60.0%
GACACATCTCGACCGTCCCT+CGG - chr7_4:43937710-43937729 Msa1155950:CDS 60.0%
CCTAGTGGGAAACCGAGGGA+CGG + chr7_4:43937725-43937744 None:intergenic 60.0%
!! GGTGTTGATGCCAGGGGATG+AGG - chr7_4:43938222-43938241 Msa1155950:CDS 60.0%
ATGCTGACACGGGCAAGGCA+TGG + chr7_4:43938263-43938282 None:intergenic 60.0%
CTTGCCCGTGTCAGCATTCG+CGG - chr7_4:43938266-43938285 Msa1155950:CDS 60.0%
CTAGCCGCGAATGCTGACAC+GGG + chr7_4:43938273-43938292 None:intergenic 60.0%
!! GATCACGGTGGTTTGGTCGG+TGG + chr7_4:43938322-43938341 None:intergenic 60.0%
GCGGGAAAGGAAAGTAGGCG+TGG + chr7_4:43937438-43937457 None:intergenic 60.0%
GACACATCTCGACCGTCCCT+CGG - chr7_4:43937710-43937729 Msa1155950:CDS 60.0%
CCTAGTGGGAAACCGAGGGA+CGG + chr7_4:43937725-43937744 None:intergenic 60.0%
!! GGTGTTGATGCCAGGGGATG+AGG - chr7_4:43938222-43938241 Msa1155950:CDS 60.0%
ATGCTGACACGGGCAAGGCA+TGG + chr7_4:43938263-43938282 None:intergenic 60.0%
CTTGCCCGTGTCAGCATTCG+CGG - chr7_4:43938266-43938285 Msa1155950:CDS 60.0%
CTAGCCGCGAATGCTGACAC+GGG + chr7_4:43938273-43938292 None:intergenic 60.0%
!! GATCACGGTGGTTTGGTCGG+TGG + chr7_4:43938322-43938341 None:intergenic 60.0%
CCGTCCCTCGGTTTCCCACT+AGG - chr7_4:43937722-43937741 Msa1155950:CDS 65.0%
CGCGAATGCTGACACGGGCA+AGG + chr7_4:43938268-43938287 None:intergenic 65.0%
CCGTCCCTCGGTTTCCCACT+AGG - chr7_4:43937722-43937741 Msa1155950:CDS 65.0%
CGCGAATGCTGACACGGGCA+AGG + chr7_4:43938268-43938287 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr7_4 gene 43937342 43938678 43937342 ID=Msa1155950;Name=Msa1155950
chr7_4 mRNA 43937342 43938678 43937342 ID=Msa1155950-mRNA-1;Parent=Msa1155950;Name=Msa1155950-mRNA-1;_AED=0.00;_eAED=0.00;_QI=329|1|1|1|1|1|2|339|128
chr7_4 exon 43937342 43937833 43937342 ID=Msa1155950-mRNA-1:exon:11185;Parent=Msa1155950-mRNA-1
chr7_4 exon 43938116 43938678 43938116 ID=Msa1155950-mRNA-1:exon:11184;Parent=Msa1155950-mRNA-1
chr7_4 five_prime_UTR 43938350 43938678 43938350 ID=Msa1155950-mRNA-1:five_prime_utr;Parent=Msa1155950-mRNA-1
chr7_4 CDS 43938116 43938349 43938116 ID=Msa1155950-mRNA-1:cds;Parent=Msa1155950-mRNA-1
chr7_4 CDS 43937681 43937833 43937681 ID=Msa1155950-mRNA-1:cds;Parent=Msa1155950-mRNA-1
chr7_4 three_prime_UTR 43937342 43937680 43937342 ID=Msa1155950-mRNA-1:three_prime_utr;Parent=Msa1155950-mRNA-1
Gene Sequence

>Msa1155950

ATGATGATGGAGAGCCCAGATCTTTCCCCACCTCAAGTAGACACATCTCGACCGTCCCTCGGTTTCCCACTAGGTACTGCTCTCCTCTTGCTCATCATCTTCAGCTTAAGTGGTATCTTCTCATGTTGCTACCACTGGGAAAAGTTTCGTTCACTTCATCAATCTCTCTCTCATCTTGAGGCTCCACAAGCACATACTCAATCCTCACCTTCCAAGTCCAAATCTCACTCCACGGAGATGGAAGGGGGAAAGAAGGGGCAGAGTGTGACGGTGTTGATGCCAGGGGATGAGGTACCGAAGTTTATAGCCATGCCTTGCCCGTGTCAGCATTCGCGGCTAGAAGAGATTATTGTCAACATTGAGAAGCCACCGACCAAACCACCGTGA
Protein sequence

>Msa1155950

MMMESPDLSPPQVDTSRPSLGFPLGTALLLLIIFSLSGIFSCCYHWEKFRSLHQSLSHLEAPQAHTQSSPSKSKSHSTEMEGGKKGQSVTVLMPGDEVPKFIAMPCPCQHSRLEEIIVNIEKPPTKPP*