AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1066590

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1066590 MtrunA17_Chr5g0411511 93.750 128 8 0 1 128 2 129 6.80e-77 223
Msa1066590 MtrunA17_Chr8g0366891 66.116 121 35 4 3 122 1 116 2.80e-49 154
Msa1066590 MtrunA17_Chr7g0249111 80.597 67 2 1 1 67 1 56 1.98e-22 83.6
Msa1066590 MtrunA17_Chr5g0428121 36.522 115 68 2 16 126 9 122 4.16e-12 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1066590 AT5G65660.1 58.268 127 51 2 2 128 1 125 5.79e-46 145

Find 83 sgRNAs with CRISPR-Local

Find 199 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAAAGGGTGGCAAGCAAAA+TGG 0.271852 7_2:-60614203 None:intergenic
GAAGGATTAACTATAACTTA+TGG 0.283702 7_2:-60614245 None:intergenic
TATTACCGTGGAGTGAGATT+TGG 0.322108 7_2:-60614600 None:intergenic
TTGCCACCTGCATAATCCTT+TGG 0.358271 7_2:-60615247 None:intergenic
AAAATACTACTACTCATATA+TGG 0.365240 7_2:-60614294 None:intergenic
GGGATATTTAAACACTTTAG+AGG 0.376848 7_2:-60614126 None:intergenic
AAGGATTAACTATAACTTAT+GGG 0.387964 7_2:-60614244 None:intergenic
ACTTGAGGTGGGGAAAGATC+TGG 0.392706 7_2:-60614398 None:intergenic
GGAGATGGAAGGGGAAAAGA+AGG 0.393491 7_2:+60614898 Msa1066590:intron
CTCATCATCTTCAGCTTAAG+TGG 0.396808 7_2:+60614473 Msa1066590:CDS
TGTAGTTGGTGAAGTGTTGC+GGG 0.397253 7_2:-60614165 None:intergenic
CTTGCCCGTGTCAGCATTCG+CGG 0.412175 7_2:+60614978 Msa1066590:CDS
GGAAAGTAAAGAAACTTTCA+TGG 0.421053 7_2:-60614072 None:intergenic
GTGTGTCGCTGAGGAAATTA+AGG 0.425009 7_2:-60614267 None:intergenic
TTCGTCGGGACAAATGTGTT+TGG 0.425626 7_2:-60615204 None:intergenic
CGAACTGTATTTGTAGTTTG+CGG 0.425667 7_2:+60615352 Msa1066590:three_prime_UTR
GAAAGGGTGGCAAGCAAAAT+GGG 0.433378 7_2:-60614202 None:intergenic
TCTTCTCATGTTGCTACCAC+TGG 0.447898 7_2:+60614498 Msa1066590:CDS
AGTGTGACGGTGTTGATGCC+AGG 0.456740 7_2:+60614926 Msa1066590:CDS
ATGATTTATATGAAATAGAA+AGG 0.457860 7_2:-60614219 None:intergenic
TTGGTGAAGTGTTGCGGGAA+AGG 0.458988 7_2:-60614160 None:intergenic
AAAGAAAAGGATTGTGTAGT+TGG 0.472421 7_2:-60614179 None:intergenic
CAATGGTGATCACGGTGGTT+TGG 0.473390 7_2:-60615038 None:intergenic
GGGTGTATCAACTAGAGAAA+AGG 0.480576 7_2:-60614105 None:intergenic
CCGTCCCTCGGTTTCCCACT+AGG 0.483894 7_2:+60614434 Msa1066590:CDS
CGTGGAGTGAGATTTGGACT+TGG 0.508123 7_2:-60614594 None:intergenic
CTTGAGGTGGGGAAAGATCT+GGG 0.508977 7_2:-60614397 None:intergenic
CTTCCTACTGTCTCGTGTGT+AGG 0.509249 7_2:+60615316 Msa1066590:three_prime_UTR
CGAGATGTGTCTACTTGAGG+TGG 0.510749 7_2:-60614410 None:intergenic
CAAGCAAAATGGGAAAGAAA+AGG 0.516215 7_2:-60614192 None:intergenic
AGATTTGGACTTGGAAGGTG+AGG 0.516452 7_2:-60614585 None:intergenic
ATGCTGACACGGGCAAGGCA+TGG 0.518272 7_2:-60614972 None:intergenic
GTGTAGTTGGTGAAGTGTTG+CGG 0.522503 7_2:-60614166 None:intergenic
GGTGTTGATGCCAGGGGATG+AGG 0.522735 7_2:+60614934 Msa1066590:CDS
TCAATCTCTCTCTCATCTTG+AGG 0.522920 7_2:+60614541 Msa1066590:CDS
GACACATCTCGACCGTCCCT+CGG 0.527794 7_2:+60614422 Msa1066590:CDS
AACTTCGGTACCTCATCCCC+TGG 0.529530 7_2:-60614944 None:intergenic
CGCGAATGCTGACACGGGCA+AGG 0.530171 7_2:-60614977 None:intergenic
GAGATGGAAGGGGAAAAGAA+GGG 0.530732 7_2:+60614899 Msa1066590:CDS
AGATGGAAGGGGAAAAGAAG+GGG 0.531883 7_2:+60614900 Msa1066590:CDS
AGCTGAAGATGATGAGCAAG+AGG 0.534577 7_2:-60614466 None:intergenic
TATCAAAGGTAATATGATGA+TGG 0.538330 7_2:+60614370 Msa1066590:exon
GGTGATCACGGTGGTTTGGT+CGG 0.540559 7_2:-60615034 None:intergenic
TCTAGCCGCGAATGCTGACA+CGG 0.546125 7_2:-60614983 None:intergenic
CTTCTCATGTTGCTACCACT+GGG 0.549662 7_2:+60614499 Msa1066590:CDS
AAAAGACCAAAGGATTATGC+AGG 0.554133 7_2:+60615241 Msa1066590:three_prime_UTR
GGATATTTAAACACTTTAGA+GGG 0.560090 7_2:-60614125 None:intergenic
GATCACGGTGGTTTGGTCGG+TGG 0.567091 7_2:-60615031 None:intergenic
TTGGTGGATAGGAGATGGAA+GGG 0.567734 7_2:+60614888 Msa1066590:intron
GTTGGTGGATAGGAGATGGA+AGG 0.570134 7_2:+60614887 Msa1066590:intron
GGTCGAGATGTGTCTACTTG+AGG 0.570607 7_2:-60614413 None:intergenic
TTTATATGAAATAGAAAGGG+TGG 0.571866 7_2:-60614215 None:intergenic
GTGTTGCGGGAAAGGAAAGT+AGG 0.573190 7_2:-60614152 None:intergenic
ACATACTACAATGGTGATCA+CGG 0.583414 7_2:-60615046 None:intergenic
TAGAGAAAAGGAGCAAGTTG+AGG 0.583790 7_2:-60614093 None:intergenic
GAGGATTGAGTATGTGCTTG+TGG 0.589598 7_2:-60614566 None:intergenic
GAGATGTGTCTACTTGAGGT+GGG 0.592423 7_2:-60614409 None:intergenic
AAAGGATTATGCAGGTGGCA+AGG 0.602551 7_2:+60615249 Msa1066590:three_prime_UTR
TGATTTATATGAAATAGAAA+GGG 0.602838 7_2:-60614218 None:intergenic
TGGTGGATAGGAGATGGAAG+GGG 0.602910 7_2:+60614889 Msa1066590:intron
GCGGGAAAGGAAAGTAGGCG+TGG 0.603747 7_2:-60614147 None:intergenic
CATGTATTCTTCTATACACA+TGG 0.605759 7_2:-60615098 None:intergenic
AGAGGAGAGCAGTACCTAGT+GGG 0.605822 7_2:-60614448 None:intergenic
CTAGCCGCGAATGCTGACAC+GGG 0.608171 7_2:-60614982 None:intergenic
GTGTGACGGTGTTGATGCCA+GGG 0.609269 7_2:+60614927 Msa1066590:CDS
CAAGGCATGGCTATAAACTT+CGG 0.609997 7_2:-60614959 None:intergenic
CGGGAAAGGAAAGTAGGCGT+GGG 0.617775 7_2:-60614146 None:intergenic
GAGTGAGATTTGGACTTGGA+AGG 0.621951 7_2:-60614590 None:intergenic
AAGTTATAAGAGCTTATCAA+AGG 0.623107 7_2:+60614356 Msa1066590:five_prime_UTR
CAGTACCTAGTGGGAAACCG+AGG 0.623236 7_2:-60614439 None:intergenic
AGACCAAAGGATTATGCAGG+TGG 0.626537 7_2:+60615244 Msa1066590:three_prime_UTR
CAAGTCCAAATCTCACTCCA+CGG 0.634220 7_2:+60614595 Msa1066590:CDS
AAAGAAGGGGCAGAGTGTGA+CGG 0.635702 7_2:+60614913 Msa1066590:CDS
AGATGTGTCTACTTGAGGTG+GGG 0.637020 7_2:-60614408 None:intergenic
AAGAGGAGAGCAGTACCTAG+TGG 0.638253 7_2:-60614449 None:intergenic
GTGCCTACACACGAGACAGT+AGG 0.642189 7_2:-60615319 None:intergenic
GTCGCTGAGGAAATTAAGGA+AGG 0.643661 7_2:-60614263 None:intergenic
CGATAATTAACATACTACAA+TGG 0.657602 7_2:-60615055 None:intergenic
AGTACCTAGTGGGAAACCGA+GGG 0.682252 7_2:-60614438 None:intergenic
CCTAGTGGGAAACCGAGGGA+CGG 0.686417 7_2:-60614434 None:intergenic
TATGGATGTGTGTGTCGCTG+AGG 0.711993 7_2:-60614276 None:intergenic
TGTGACGGTGTTGATGCCAG+GGG 0.749010 7_2:+60614928 Msa1066590:CDS
TACTACAATGGTGATCACGG+TGG 0.756673 7_2:-60615043 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGATTTATATGAAATAGAAA+GGG - chr7_2:60614221-60614240 None:intergenic 15.0%
!! ATGATTTATATGAAATAGAA+AGG - chr7_2:60614222-60614241 None:intergenic 15.0%
!!! AATTGATTATAATTTTCTCT+TGG - chr7_2:60614735-60614754 None:intergenic 15.0%
!!! AATTTTACAATTTTGTTTGT+TGG + chr7_2:60614869-60614888 Msa1066590:intron 15.0%
!!! TTATAATATATCTTTTTCGT+CGG - chr7_2:60615222-60615241 None:intergenic 15.0%
!! ATATATTATAAAAAGACCAA+AGG + chr7_2:60615231-60615250 Msa1066590:three_prime_UTR 15.0%
!! TGATTTATATGAAATAGAAA+GGG - chr7_2:60614221-60614240 None:intergenic 15.0%
!! ATGATTTATATGAAATAGAA+AGG - chr7_2:60614222-60614241 None:intergenic 15.0%
!!! AATTGATTATAATTTTCTCT+TGG - chr7_2:60614735-60614754 None:intergenic 15.0%
!!! AATTTTACAATTTTGTTTGT+TGG + chr7_2:60614869-60614888 Msa1066590:intron 15.0%
!!! TTATAATATATCTTTTTCGT+CGG - chr7_2:60615222-60615241 None:intergenic 15.0%
!! ATATATTATAAAAAGACCAA+AGG + chr7_2:60615231-60615250 Msa1066590:three_prime_UTR 15.0%
!! AAGGATTAACTATAACTTAT+GGG - chr7_2:60614247-60614266 None:intergenic 20.0%
!! AAAATACTACTACTCATATA+TGG - chr7_2:60614297-60614316 None:intergenic 20.0%
!!! TATAATATATCTTTTTCGTC+GGG - chr7_2:60615221-60615240 None:intergenic 20.0%
!! AAGGATTAACTATAACTTAT+GGG - chr7_2:60614247-60614266 None:intergenic 20.0%
!! AAAATACTACTACTCATATA+TGG - chr7_2:60614297-60614316 None:intergenic 20.0%
!!! TATAATATATCTTTTTCGTC+GGG - chr7_2:60615221-60615240 None:intergenic 20.0%
! GGATATTTAAACACTTTAGA+GGG - chr7_2:60614128-60614147 None:intergenic 25.0%
! TTTATATGAAATAGAAAGGG+TGG - chr7_2:60614218-60614237 None:intergenic 25.0%
! GAAGGATTAACTATAACTTA+TGG - chr7_2:60614248-60614267 None:intergenic 25.0%
! AAGTTATAAGAGCTTATCAA+AGG + chr7_2:60614356-60614375 Msa1066590:five_prime_UTR 25.0%
! TATCAAAGGTAATATGATGA+TGG + chr7_2:60614370-60614389 Msa1066590:exon 25.0%
!!! TTTACAATTTTGTTTGTTGG+TGG + chr7_2:60614872-60614891 Msa1066590:intron 25.0%
! CGATAATTAACATACTACAA+TGG - chr7_2:60615058-60615077 None:intergenic 25.0%
! GGATATTTAAACACTTTAGA+GGG - chr7_2:60614128-60614147 None:intergenic 25.0%
! TTTATATGAAATAGAAAGGG+TGG - chr7_2:60614218-60614237 None:intergenic 25.0%
! GAAGGATTAACTATAACTTA+TGG - chr7_2:60614248-60614267 None:intergenic 25.0%
! AAGTTATAAGAGCTTATCAA+AGG + chr7_2:60614356-60614375 Msa1066590:five_prime_UTR 25.0%
! TATCAAAGGTAATATGATGA+TGG + chr7_2:60614370-60614389 Msa1066590:exon 25.0%
!!! TTTACAATTTTGTTTGTTGG+TGG + chr7_2:60614872-60614891 Msa1066590:intron 25.0%
! CGATAATTAACATACTACAA+TGG - chr7_2:60615058-60615077 None:intergenic 25.0%
GGAAAGTAAAGAAACTTTCA+TGG - chr7_2:60614075-60614094 None:intergenic 30.0%
GGGATATTTAAACACTTTAG+AGG - chr7_2:60614129-60614148 None:intergenic 30.0%
AAAGAAAAGGATTGTGTAGT+TGG - chr7_2:60614182-60614201 None:intergenic 30.0%
AAAGAGTGAAATTATTACCG+TGG - chr7_2:60614615-60614634 None:intergenic 30.0%
AGGATCATACAGTTGTAAAA+TGG - chr7_2:60614774-60614793 None:intergenic 30.0%
!! AATTTTGTTTGTTGGTGGAT+AGG + chr7_2:60614877-60614896 Msa1066590:intron 30.0%
CATGTATTCTTCTATACACA+TGG - chr7_2:60615101-60615120 None:intergenic 30.0%
GGAAAGTAAAGAAACTTTCA+TGG - chr7_2:60614075-60614094 None:intergenic 30.0%
GGGATATTTAAACACTTTAG+AGG - chr7_2:60614129-60614148 None:intergenic 30.0%
AAAGAAAAGGATTGTGTAGT+TGG - chr7_2:60614182-60614201 None:intergenic 30.0%
AAAGAGTGAAATTATTACCG+TGG - chr7_2:60614615-60614634 None:intergenic 30.0%
AGGATCATACAGTTGTAAAA+TGG - chr7_2:60614774-60614793 None:intergenic 30.0%
!! AATTTTGTTTGTTGGTGGAT+AGG + chr7_2:60614877-60614896 Msa1066590:intron 30.0%
CATGTATTCTTCTATACACA+TGG - chr7_2:60615101-60615120 None:intergenic 30.0%
CAAGCAAAATGGGAAAGAAA+AGG - chr7_2:60614195-60614214 None:intergenic 35.0%
TTGGCGTTGTAAGTCAAAAA+AGG - chr7_2:60614794-60614813 None:intergenic 35.0%
TAGTGCTAAAAGCTTGACAT+TGG - chr7_2:60614813-60614832 None:intergenic 35.0%
ACATACTACAATGGTGATCA+CGG - chr7_2:60615049-60615068 None:intergenic 35.0%
AAAAGACCAAAGGATTATGC+AGG + chr7_2:60615241-60615260 Msa1066590:three_prime_UTR 35.0%
CGAACTGTATTTGTAGTTTG+CGG + chr7_2:60615352-60615371 Msa1066590:three_prime_UTR 35.0%
CAAGCAAAATGGGAAAGAAA+AGG - chr7_2:60614195-60614214 None:intergenic 35.0%
TTGGCGTTGTAAGTCAAAAA+AGG - chr7_2:60614794-60614813 None:intergenic 35.0%
TAGTGCTAAAAGCTTGACAT+TGG - chr7_2:60614813-60614832 None:intergenic 35.0%
ACATACTACAATGGTGATCA+CGG - chr7_2:60615049-60615068 None:intergenic 35.0%
AAAAGACCAAAGGATTATGC+AGG + chr7_2:60615241-60615260 Msa1066590:three_prime_UTR 35.0%
CGAACTGTATTTGTAGTTTG+CGG + chr7_2:60615352-60615371 Msa1066590:three_prime_UTR 35.0%
TAGAGAAAAGGAGCAAGTTG+AGG - chr7_2:60614096-60614115 None:intergenic 40.0%
GGGTGTATCAACTAGAGAAA+AGG - chr7_2:60614108-60614127 None:intergenic 40.0%
CTCATCATCTTCAGCTTAAG+TGG + chr7_2:60614473-60614492 Msa1066590:CDS 40.0%
TCAATCTCTCTCTCATCTTG+AGG + chr7_2:60614541-60614560 Msa1066590:CDS 40.0%
TATTACCGTGGAGTGAGATT+TGG - chr7_2:60614603-60614622 None:intergenic 40.0%
CAAGGCATGGCTATAAACTT+CGG - chr7_2:60614962-60614981 None:intergenic 40.0%
TAGAGAAAAGGAGCAAGTTG+AGG - chr7_2:60614096-60614115 None:intergenic 40.0%
GGGTGTATCAACTAGAGAAA+AGG - chr7_2:60614108-60614127 None:intergenic 40.0%
CTCATCATCTTCAGCTTAAG+TGG + chr7_2:60614473-60614492 Msa1066590:CDS 40.0%
TCAATCTCTCTCTCATCTTG+AGG + chr7_2:60614541-60614560 Msa1066590:CDS 40.0%
TATTACCGTGGAGTGAGATT+TGG - chr7_2:60614603-60614622 None:intergenic 40.0%
CAAGGCATGGCTATAAACTT+CGG - chr7_2:60614962-60614981 None:intergenic 40.0%
!! TGTAGTTGGTGAAGTGTTGC+GGG - chr7_2:60614168-60614187 None:intergenic 45.0%
!! GTGTAGTTGGTGAAGTGTTG+CGG - chr7_2:60614169-60614188 None:intergenic 45.0%
GAAAGGGTGGCAAGCAAAAT+GGG - chr7_2:60614205-60614224 None:intergenic 45.0%
AGAAAGGGTGGCAAGCAAAA+TGG - chr7_2:60614206-60614225 None:intergenic 45.0%
GTCGCTGAGGAAATTAAGGA+AGG - chr7_2:60614266-60614285 None:intergenic 45.0%
! GTGTGTCGCTGAGGAAATTA+AGG - chr7_2:60614270-60614289 None:intergenic 45.0%
!!! TGTTTTGATGCTGATGCTGG+TGG - chr7_2:60614333-60614352 None:intergenic 45.0%
!!! GCTTGTTTTGATGCTGATGC+TGG - chr7_2:60614336-60614355 None:intergenic 45.0%
AGATGTGTCTACTTGAGGTG+GGG - chr7_2:60614411-60614430 None:intergenic 45.0%
GAGATGTGTCTACTTGAGGT+GGG - chr7_2:60614412-60614431 None:intergenic 45.0%
AGCTGAAGATGATGAGCAAG+AGG - chr7_2:60614469-60614488 None:intergenic 45.0%
TCTTCTCATGTTGCTACCAC+TGG + chr7_2:60614498-60614517 Msa1066590:CDS 45.0%
CTTCTCATGTTGCTACCACT+GGG + chr7_2:60614499-60614518 Msa1066590:CDS 45.0%
! GTGAACGAAACTTTTCCCAG+TGG - chr7_2:60614517-60614536 None:intergenic 45.0%
GAGGATTGAGTATGTGCTTG+TGG - chr7_2:60614569-60614588 None:intergenic 45.0%
AGATTTGGACTTGGAAGGTG+AGG - chr7_2:60614588-60614607 None:intergenic 45.0%
GAGTGAGATTTGGACTTGGA+AGG - chr7_2:60614593-60614612 None:intergenic 45.0%
CAAGTCCAAATCTCACTCCA+CGG + chr7_2:60614595-60614614 Msa1066590:CDS 45.0%
! GTTTGTTGGTGGATAGGAGA+TGG + chr7_2:60614883-60614902 Msa1066590:intron 45.0%
! TTGGTGGATAGGAGATGGAA+GGG + chr7_2:60614888-60614907 Msa1066590:intron 45.0%
GAGATGGAAGGGGAAAAGAA+GGG + chr7_2:60614899-60614918 Msa1066590:CDS 45.0%
AGATGGAAGGGGAAAAGAAG+GGG + chr7_2:60614900-60614919 Msa1066590:CDS 45.0%
TACTACAATGGTGATCACGG+TGG - chr7_2:60615046-60615065 None:intergenic 45.0%
TTCGTCGGGACAAATGTGTT+TGG - chr7_2:60615207-60615226 None:intergenic 45.0%
AGACCAAAGGATTATGCAGG+TGG + chr7_2:60615244-60615263 Msa1066590:three_prime_UTR 45.0%
TTGCCACCTGCATAATCCTT+TGG - chr7_2:60615250-60615269 None:intergenic 45.0%
AAAGGATTATGCAGGTGGCA+AGG + chr7_2:60615249-60615268 Msa1066590:three_prime_UTR 45.0%
!! TGTAGTTGGTGAAGTGTTGC+GGG - chr7_2:60614168-60614187 None:intergenic 45.0%
!! GTGTAGTTGGTGAAGTGTTG+CGG - chr7_2:60614169-60614188 None:intergenic 45.0%
GAAAGGGTGGCAAGCAAAAT+GGG - chr7_2:60614205-60614224 None:intergenic 45.0%
AGAAAGGGTGGCAAGCAAAA+TGG - chr7_2:60614206-60614225 None:intergenic 45.0%
GTCGCTGAGGAAATTAAGGA+AGG - chr7_2:60614266-60614285 None:intergenic 45.0%
! GTGTGTCGCTGAGGAAATTA+AGG - chr7_2:60614270-60614289 None:intergenic 45.0%
!!! TGTTTTGATGCTGATGCTGG+TGG - chr7_2:60614333-60614352 None:intergenic 45.0%
!!! GCTTGTTTTGATGCTGATGC+TGG - chr7_2:60614336-60614355 None:intergenic 45.0%
AGATGTGTCTACTTGAGGTG+GGG - chr7_2:60614411-60614430 None:intergenic 45.0%
GAGATGTGTCTACTTGAGGT+GGG - chr7_2:60614412-60614431 None:intergenic 45.0%
AGCTGAAGATGATGAGCAAG+AGG - chr7_2:60614469-60614488 None:intergenic 45.0%
TCTTCTCATGTTGCTACCAC+TGG + chr7_2:60614498-60614517 Msa1066590:CDS 45.0%
CTTCTCATGTTGCTACCACT+GGG + chr7_2:60614499-60614518 Msa1066590:CDS 45.0%
! GTGAACGAAACTTTTCCCAG+TGG - chr7_2:60614517-60614536 None:intergenic 45.0%
GAGGATTGAGTATGTGCTTG+TGG - chr7_2:60614569-60614588 None:intergenic 45.0%
AGATTTGGACTTGGAAGGTG+AGG - chr7_2:60614588-60614607 None:intergenic 45.0%
GAGTGAGATTTGGACTTGGA+AGG - chr7_2:60614593-60614612 None:intergenic 45.0%
CAAGTCCAAATCTCACTCCA+CGG + chr7_2:60614595-60614614 Msa1066590:CDS 45.0%
! GTTTGTTGGTGGATAGGAGA+TGG + chr7_2:60614883-60614902 Msa1066590:intron 45.0%
! TTGGTGGATAGGAGATGGAA+GGG + chr7_2:60614888-60614907 Msa1066590:intron 45.0%
GAGATGGAAGGGGAAAAGAA+GGG + chr7_2:60614899-60614918 Msa1066590:CDS 45.0%
AGATGGAAGGGGAAAAGAAG+GGG + chr7_2:60614900-60614919 Msa1066590:CDS 45.0%
TACTACAATGGTGATCACGG+TGG - chr7_2:60615046-60615065 None:intergenic 45.0%
TTCGTCGGGACAAATGTGTT+TGG - chr7_2:60615207-60615226 None:intergenic 45.0%
AGACCAAAGGATTATGCAGG+TGG + chr7_2:60615244-60615263 Msa1066590:three_prime_UTR 45.0%
TTGCCACCTGCATAATCCTT+TGG - chr7_2:60615250-60615269 None:intergenic 45.0%
AAAGGATTATGCAGGTGGCA+AGG + chr7_2:60615249-60615268 Msa1066590:three_prime_UTR 45.0%
!!! ATAATTTGAAATTATAAAAT+TGG + chr7_2:60614677-60614696 Msa1066590:intron 5.0%
!!! ATAATTTGAAATTATAAAAT+TGG + chr7_2:60614677-60614696 Msa1066590:intron 5.0%
GTGTTGCGGGAAAGGAAAGT+AGG - chr7_2:60614155-60614174 None:intergenic 50.0%
!! TTGGTGAAGTGTTGCGGGAA+AGG - chr7_2:60614163-60614182 None:intergenic 50.0%
! TATGGATGTGTGTGTCGCTG+AGG - chr7_2:60614279-60614298 None:intergenic 50.0%
CTTGAGGTGGGGAAAGATCT+GGG - chr7_2:60614400-60614419 None:intergenic 50.0%
ACTTGAGGTGGGGAAAGATC+TGG - chr7_2:60614401-60614420 None:intergenic 50.0%
CGAGATGTGTCTACTTGAGG+TGG - chr7_2:60614413-60614432 None:intergenic 50.0%
GGTCGAGATGTGTCTACTTG+AGG - chr7_2:60614416-60614435 None:intergenic 50.0%
AGTACCTAGTGGGAAACCGA+GGG - chr7_2:60614441-60614460 None:intergenic 50.0%
AGAGGAGAGCAGTACCTAGT+GGG - chr7_2:60614451-60614470 None:intergenic 50.0%
AAGAGGAGAGCAGTACCTAG+TGG - chr7_2:60614452-60614471 None:intergenic 50.0%
CGTGGAGTGAGATTTGGACT+TGG - chr7_2:60614597-60614616 None:intergenic 50.0%
! GTTGGTGGATAGGAGATGGA+AGG + chr7_2:60614887-60614906 Msa1066590:intron 50.0%
! TGGTGGATAGGAGATGGAAG+GGG + chr7_2:60614889-60614908 Msa1066590:intron 50.0%
GGAGATGGAAGGGGAAAAGA+AGG + chr7_2:60614898-60614917 Msa1066590:intron 50.0%
AAAGAAGGGGCAGAGTGTGA+CGG + chr7_2:60614913-60614932 Msa1066590:CDS 50.0%
CAATGGTGATCACGGTGGTT+TGG - chr7_2:60615041-60615060 None:intergenic 50.0%
CTTCCTACTGTCTCGTGTGT+AGG + chr7_2:60615316-60615335 Msa1066590:three_prime_UTR 50.0%
GTGTTGCGGGAAAGGAAAGT+AGG - chr7_2:60614155-60614174 None:intergenic 50.0%
!! TTGGTGAAGTGTTGCGGGAA+AGG - chr7_2:60614163-60614182 None:intergenic 50.0%
! TATGGATGTGTGTGTCGCTG+AGG - chr7_2:60614279-60614298 None:intergenic 50.0%
CTTGAGGTGGGGAAAGATCT+GGG - chr7_2:60614400-60614419 None:intergenic 50.0%
ACTTGAGGTGGGGAAAGATC+TGG - chr7_2:60614401-60614420 None:intergenic 50.0%
CGAGATGTGTCTACTTGAGG+TGG - chr7_2:60614413-60614432 None:intergenic 50.0%
GGTCGAGATGTGTCTACTTG+AGG - chr7_2:60614416-60614435 None:intergenic 50.0%
AGTACCTAGTGGGAAACCGA+GGG - chr7_2:60614441-60614460 None:intergenic 50.0%
AGAGGAGAGCAGTACCTAGT+GGG - chr7_2:60614451-60614470 None:intergenic 50.0%
AAGAGGAGAGCAGTACCTAG+TGG - chr7_2:60614452-60614471 None:intergenic 50.0%
CGTGGAGTGAGATTTGGACT+TGG - chr7_2:60614597-60614616 None:intergenic 50.0%
! GTTGGTGGATAGGAGATGGA+AGG + chr7_2:60614887-60614906 Msa1066590:intron 50.0%
! TGGTGGATAGGAGATGGAAG+GGG + chr7_2:60614889-60614908 Msa1066590:intron 50.0%
GGAGATGGAAGGGGAAAAGA+AGG + chr7_2:60614898-60614917 Msa1066590:intron 50.0%
AAAGAAGGGGCAGAGTGTGA+CGG + chr7_2:60614913-60614932 Msa1066590:CDS 50.0%
CAATGGTGATCACGGTGGTT+TGG - chr7_2:60615041-60615060 None:intergenic 50.0%
CTTCCTACTGTCTCGTGTGT+AGG + chr7_2:60615316-60615335 Msa1066590:three_prime_UTR 50.0%
CGGGAAAGGAAAGTAGGCGT+GGG - chr7_2:60614149-60614168 None:intergenic 55.0%
CAGTACCTAGTGGGAAACCG+AGG - chr7_2:60614442-60614461 None:intergenic 55.0%
!! AGTGTGACGGTGTTGATGCC+AGG + chr7_2:60614926-60614945 Msa1066590:CDS 55.0%
!! GTGTGACGGTGTTGATGCCA+GGG + chr7_2:60614927-60614946 Msa1066590:CDS 55.0%
!! TGTGACGGTGTTGATGCCAG+GGG + chr7_2:60614928-60614947 Msa1066590:CDS 55.0%
AACTTCGGTACCTCATCCCC+TGG - chr7_2:60614947-60614966 None:intergenic 55.0%
TCTAGCCGCGAATGCTGACA+CGG - chr7_2:60614986-60615005 None:intergenic 55.0%
!! GGTGATCACGGTGGTTTGGT+CGG - chr7_2:60615037-60615056 None:intergenic 55.0%
GTGCCTACACACGAGACAGT+AGG - chr7_2:60615322-60615341 None:intergenic 55.0%
TGCGGAGATGCAACTACTGC+TGG + chr7_2:60615370-60615389 Msa1066590:three_prime_UTR 55.0%
CGGGAAAGGAAAGTAGGCGT+GGG - chr7_2:60614149-60614168 None:intergenic 55.0%
CAGTACCTAGTGGGAAACCG+AGG - chr7_2:60614442-60614461 None:intergenic 55.0%
!! AGTGTGACGGTGTTGATGCC+AGG + chr7_2:60614926-60614945 Msa1066590:CDS 55.0%
!! GTGTGACGGTGTTGATGCCA+GGG + chr7_2:60614927-60614946 Msa1066590:CDS 55.0%
!! TGTGACGGTGTTGATGCCAG+GGG + chr7_2:60614928-60614947 Msa1066590:CDS 55.0%
AACTTCGGTACCTCATCCCC+TGG - chr7_2:60614947-60614966 None:intergenic 55.0%
TCTAGCCGCGAATGCTGACA+CGG - chr7_2:60614986-60615005 None:intergenic 55.0%
!! GGTGATCACGGTGGTTTGGT+CGG - chr7_2:60615037-60615056 None:intergenic 55.0%
GTGCCTACACACGAGACAGT+AGG - chr7_2:60615322-60615341 None:intergenic 55.0%
GCGGGAAAGGAAAGTAGGCG+TGG - chr7_2:60614150-60614169 None:intergenic 60.0%
GACACATCTCGACCGTCCCT+CGG + chr7_2:60614422-60614441 Msa1066590:CDS 60.0%
CCTAGTGGGAAACCGAGGGA+CGG - chr7_2:60614437-60614456 None:intergenic 60.0%
!! GGTGTTGATGCCAGGGGATG+AGG + chr7_2:60614934-60614953 Msa1066590:CDS 60.0%
ATGCTGACACGGGCAAGGCA+TGG - chr7_2:60614975-60614994 None:intergenic 60.0%
CTTGCCCGTGTCAGCATTCG+CGG + chr7_2:60614978-60614997 Msa1066590:CDS 60.0%
CTAGCCGCGAATGCTGACAC+GGG - chr7_2:60614985-60615004 None:intergenic 60.0%
!! GATCACGGTGGTTTGGTCGG+TGG - chr7_2:60615034-60615053 None:intergenic 60.0%
GCGGGAAAGGAAAGTAGGCG+TGG - chr7_2:60614150-60614169 None:intergenic 60.0%
GACACATCTCGACCGTCCCT+CGG + chr7_2:60614422-60614441 Msa1066590:CDS 60.0%
CCTAGTGGGAAACCGAGGGA+CGG - chr7_2:60614437-60614456 None:intergenic 60.0%
!! GGTGTTGATGCCAGGGGATG+AGG + chr7_2:60614934-60614953 Msa1066590:CDS 60.0%
ATGCTGACACGGGCAAGGCA+TGG - chr7_2:60614975-60614994 None:intergenic 60.0%
CTTGCCCGTGTCAGCATTCG+CGG + chr7_2:60614978-60614997 Msa1066590:CDS 60.0%
CTAGCCGCGAATGCTGACAC+GGG - chr7_2:60614985-60615004 None:intergenic 60.0%
!! GATCACGGTGGTTTGGTCGG+TGG - chr7_2:60615034-60615053 None:intergenic 60.0%
CCGTCCCTCGGTTTCCCACT+AGG + chr7_2:60614434-60614453 Msa1066590:CDS 65.0%
CGCGAATGCTGACACGGGCA+AGG - chr7_2:60614980-60614999 None:intergenic 65.0%
CCGTCCCTCGGTTTCCCACT+AGG + chr7_2:60614434-60614453 Msa1066590:CDS 65.0%
CGCGAATGCTGACACGGGCA+AGG - chr7_2:60614980-60614999 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr7_2 gene 60614054 60615390 60614054 ID=Msa1066590;Name=Msa1066590
chr7_2 mRNA 60614054 60615390 60614054 ID=Msa1066590-mRNA-1;Parent=Msa1066590;Name=Msa1066590-mRNA-1;_AED=0.00;_eAED=0.00;_QI=329|1|1|1|1|1|2|339|128
chr7_2 exon 60614054 60614616 60614054 ID=Msa1066590-mRNA-1:exon:11986;Parent=Msa1066590-mRNA-1
chr7_2 exon 60614899 60615390 60614899 ID=Msa1066590-mRNA-1:exon:11987;Parent=Msa1066590-mRNA-1
chr7_2 five_prime_UTR 60614054 60614382 60614054 ID=Msa1066590-mRNA-1:five_prime_utr;Parent=Msa1066590-mRNA-1
chr7_2 CDS 60614383 60614616 60614383 ID=Msa1066590-mRNA-1:cds;Parent=Msa1066590-mRNA-1
chr7_2 CDS 60614899 60615051 60614899 ID=Msa1066590-mRNA-1:cds;Parent=Msa1066590-mRNA-1
chr7_2 three_prime_UTR 60615052 60615390 60615052 ID=Msa1066590-mRNA-1:three_prime_utr;Parent=Msa1066590-mRNA-1
Gene Sequence

>Msa1066590

ATGATGATGGAGAGCCCAGATCTTTCCCCACCTCAAGTAGACACATCTCGACCGTCCCTCGGTTTCCCACTAGGTACTGCTCTCCTCTTGCTCATCATCTTCAGCTTAAGTGGTATCTTCTCATGTTGCTACCACTGGGAAAAGTTTCGTTCACTTCATCAATCTCTCTCTCATCTTGAGGCTCCACAAGCACATACTCAATCCTCACCTTCCAAGTCCAAATCTCACTCCACGGAGATGGAAGGGGAAAAGAAGGGGCAGAGTGTGACGGTGTTGATGCCAGGGGATGAGGTACCGAAGTTTATAGCCATGCCTTGCCCGTGTCAGCATTCGCGGCTAGAAGAGATTATTGTCAACATTGAGAAGCCACCGACCAAACCACCGTGA
Protein sequence

>Msa1066590

MMMESPDLSPPQVDTSRPSLGFPLGTALLLLIIFSLSGIFSCCYHWEKFRSLHQSLSHLEAPQAHTQSSPSKSKSHSTEMEGEKKGQSVTVLMPGDEVPKFIAMPCPCQHSRLEEIIVNIEKPPTKPP*