AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1028500

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 100 sgRNAs with CRISPR-Local

Find 250 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACAGGTTCCATTCACTATTT+TGG 0.089427 7_1:+61451661 Msa1028500:intron
ATACCTCAGCCTCATCATTT+TGG 0.183295 7_1:+61452586 Msa1028500:five_prime_UTR
GTTGAGGGTGAGATTGAAAT+TGG 0.243871 7_1:-61450954 None:intergenic
TTGAGGCAGGGTTATGCTTT+TGG 0.248771 7_1:+61452542 Msa1028500:five_prime_UTR
GGTGAGATTGGGAGGGTGTT+AGG 0.259018 7_1:-61450842 None:intergenic
ATTGAGGGTGAGATTGAAAT+TGG 0.287143 7_1:-61450866 None:intergenic
CCATCTCTTTGATCCACTTT+AGG 0.305999 7_1:-61454371 None:intergenic
CTCTTCCACAAACCGGAATA+AGG 0.310850 7_1:-61454541 None:intergenic
TCCAAACTTATCCAGCAAAA+TGG 0.312997 7_1:-61451687 None:intergenic
GTTCCTTTGGTTCCTTATTC+CGG 0.315921 7_1:+61454529 Msa1028500:CDS
TGGAATTGCTTTCTTTCTTA+AGG 0.316427 7_1:+61451850 Msa1028500:five_prime_UTR
TGAAGGAGGGTCGACTTGTT+GGG 0.322374 7_1:+61454242 Msa1028500:intron
GAGATTGAAATGGAGGCATT+AGG 0.323129 7_1:-61450804 None:intergenic
GTTCTCTGTGGCTGTTCCTT+TGG 0.326571 7_1:+61454516 Msa1028500:CDS
TCCTTCGCGATTCTTCTTCA+AGG 0.341884 7_1:+61451157 Msa1028500:five_prime_UTR
CCCATTGCTTCTGCCGTTAG+TGG 0.350372 7_1:-61454336 None:intergenic
TCAACAAGTGGAACATTTGT+AGG 0.364179 7_1:+61451815 Msa1028500:five_prime_UTR
AAGGTTGGGCTGCTTGTGTT+TGG 0.367926 7_1:+61452610 Msa1028500:five_prime_UTR
TTACTTCGCGATGGTGTGTT+TGG 0.372301 7_1:+61451094 Msa1028500:five_prime_UTR
AGATTGAAATGGAGGCATTA+GGG 0.380645 7_1:-61450803 None:intergenic
CTTATTCCGGTTTGTGGAAG+AGG 0.384022 7_1:+61454542 Msa1028500:CDS
AAACTAATTATCATAATATT+CGG 0.388414 7_1:-61454614 None:intergenic
ATTGAAATTGGAGGTGAGAT+TGG 0.389915 7_1:-61450854 None:intergenic
GCTGTATTATTCCATGGATC+AGG 0.397740 7_1:+61451945 Msa1028500:five_prime_UTR
GATAGAGAGTGAGATTGAAA+TGG 0.408383 7_1:-61450814 None:intergenic
TTGAAGGAGGGTCGACTTGT+TGG 0.409065 7_1:+61454241 Msa1028500:intron
TTGAAATTGGAGGTGAGATT+GGG 0.411999 7_1:-61450853 None:intergenic
TTGAGGGTGAGATTGAAATT+GGG 0.413088 7_1:-61450953 None:intergenic
TGGTTCCTTATTCCGGTTTG+TGG 0.432975 7_1:+61454536 Msa1028500:CDS
ACCACGTGCAGAAGCAGGTT+GGG 0.433448 7_1:-61451126 None:intergenic
GATCAGGATCAGAACTTGCT+TGG 0.450555 7_1:+61451961 Msa1028500:five_prime_UTR
GAGGGAGAGATTCATGTTGA+GGG 0.470478 7_1:-61450894 None:intergenic
CATGTTGAGGGTTGAAATTG+AGG 0.471754 7_1:-61450882 None:intergenic
AACTTTGCTGTATTATTCCA+TGG 0.473341 7_1:+61451939 Msa1028500:five_prime_UTR
ACAAATATTATAATGATATG+TGG 0.474463 7_1:+61454715 Msa1028500:three_prime_UTR
TGAGGGAGAGATTCATGTTG+AGG 0.484884 7_1:-61450895 None:intergenic
ACTCCTCTTCTCCGTTACTA+AGG 0.485208 7_1:+61452700 Msa1028500:five_prime_UTR
ACTCCTCTTCTCCGTTACTA+AGG 0.485208 7_1:+61452746 Msa1028500:five_prime_UTR
AGAGAGTGAGATTGAAATGG+AGG 0.485227 7_1:-61450811 None:intergenic
TTTCCTCCTCTTCCACAAAC+CGG 0.491861 7_1:-61454548 None:intergenic
GAAGAATCGCGAAGGAATGA+AGG 0.495389 7_1:-61451150 None:intergenic
TTACCTTGAGAAAGTGAAGT+AGG 0.497019 7_1:-61451407 None:intergenic
ATGGCTCCCAAAATAGTGAA+TGG 0.498993 7_1:-61451668 None:intergenic
TCGTTAATCTGAGAAAACTT+TGG 0.501340 7_1:+61451016 Msa1028500:five_prime_UTR
AAGGAACCACGTGCAGAAGC+AGG 0.503698 7_1:-61451131 None:intergenic
CTGTCTCAGATTCATGTTGA+GGG 0.504404 7_1:-61450969 None:intergenic
TGAGGGTGAGATTGAAATTG+GGG 0.506801 7_1:-61450952 None:intergenic
AGGGTCGACTTGTTGGGGAC+TGG 0.507394 7_1:+61454248 Msa1028500:five_prime_UTR
ATCAGGATCAGAACTTGCTT+GGG 0.507458 7_1:+61451962 Msa1028500:five_prime_UTR
CTTTCTCAATTTCATTCCCA+CGG 0.511358 7_1:+61450765 Msa1028500:five_prime_UTR
TGCTTGGGTAATAATCTATG+AGG 0.516623 7_1:+61451977 Msa1028500:five_prime_UTR
CATGTTGACAGTTGAAATTG+AGG 0.518206 7_1:-61450913 None:intergenic
CCACTAACGGCAGAAGCAAT+GGG 0.519853 7_1:+61454336 Msa1028500:exon
TTTGTGGAAGAGGAGGAAAT+AGG 0.521010 7_1:+61454552 Msa1028500:CDS
ACCACTAACGGCAGAAGCAA+TGG 0.523106 7_1:+61454335 Msa1028500:exon
GAAGGAGGGTCGACTTGTTG+GGG 0.526793 7_1:+61454243 Msa1028500:intron
GAGGCTGAGGTATACACAAA+TGG 0.531607 7_1:-61452576 None:intergenic
TGGAAGAAGACCGCTTATAA+CGG 0.539392 7_1:+61451706 Msa1028500:five_prime_UTR
TTTGATCTTGATCTCCCTCT+CGG 0.553033 7_1:-61454586 None:intergenic
GTCGACTTGTTGGGGACTGG+TGG 0.559897 7_1:+61454251 Msa1028500:five_prime_UTR
GATTGAGATGGTGCCTAAAG+TGG 0.560564 7_1:+61454358 Msa1028500:CDS
TTCCCAAAATGATGAGGCTG+AGG 0.560951 7_1:-61452589 None:intergenic
TATAAAGACATCACCACTAA+CGG 0.563335 7_1:+61454323 Msa1028500:five_prime_UTR
AAATTGGAGGTGAGATTGGG+AGG 0.564532 7_1:-61450850 None:intergenic
CTGGTGGCTCGCAACTGTGA+AGG 0.565547 7_1:+61454267 Msa1028500:five_prime_UTR
CAAGTTCTGATCCTGATCCA+TGG 0.566576 7_1:-61451956 None:intergenic
AAACCGGAATAAGGAACCAA+AGG 0.569547 7_1:-61454532 None:intergenic
AACAGATTTACCGTTATAAG+CGG 0.575668 7_1:-61451716 None:intergenic
CGTGGGAATGAAATTGAGAA+AGG 0.577048 7_1:-61450764 None:intergenic
ATGTTGACAGTTGAAATTGA+GGG 0.578438 7_1:-61450912 None:intergenic
ATGTTGAGGGTTGAAATTGA+GGG 0.579664 7_1:-61450881 None:intergenic
CAGAAGCAATGGGATTGAGA+TGG 0.580010 7_1:+61454346 Msa1028500:exon
TTGTGAACGTGACTGCTCGA+TGG 0.580508 7_1:+61452646 Msa1028500:five_prime_UTR
TGCATGTAGGCTTCAACAAG+TGG 0.583443 7_1:+61451803 Msa1028500:intron
AAATTGAGAAAGGTGGAGAG+AGG 0.585921 7_1:-61450754 None:intergenic
ACCCAACCTGCTTCTGCACG+TGG 0.587590 7_1:+61451125 Msa1028500:five_prime_UTR
TCGCCTACTTCACTTTCTCA+AGG 0.591636 7_1:+61451404 Msa1028500:five_prime_UTR
ATTCCGGTTTGTGGAAGAGG+AGG 0.595618 7_1:+61454545 Msa1028500:CDS
CAGTCACGTTCACAAAGAAG+AGG 0.597794 7_1:-61452638 None:intergenic
AATTGGAGGTGAGATTGGGA+GGG 0.602277 7_1:-61450849 None:intergenic
AACCACGTGCAGAAGCAGGT+TGG 0.602872 7_1:-61451127 None:intergenic
TACGTGGTTAACCTTAGTAA+CGG 0.606047 7_1:-61452711 None:intergenic
TAACCTTAGTAACGGAGAAG+AGG 0.608222 7_1:-61452703 None:intergenic
TAACCTTAGTAACGGAGAAG+AGG 0.608222 7_1:-61452749 None:intergenic
GAGGGTGAGATTGAAATTGG+AGG 0.608950 7_1:-61450863 None:intergenic
GGGAATGAAATTGAGAAAGG+TGG 0.612757 7_1:-61450761 None:intergenic
GCTGTCTCAGATTCATGTTG+AGG 0.617374 7_1:-61450970 None:intergenic
TATGTGGTTAACCTTAGTAA+CGG 0.618186 7_1:-61452757 None:intergenic
TTCGTCAAGGCATTTCGCCG+TGG 0.619990 7_1:+61451368 Msa1028500:five_prime_UTR
CCTAAAGTGGATCAAAGAGA+TGG 0.624012 7_1:+61454371 Msa1028500:CDS
GTAACGAAGTAACGTCGTTG+TGG 0.624471 7_1:-61451075 None:intergenic
GTTACTTCGTTACTTCGCGA+TGG 0.627642 7_1:+61451085 Msa1028500:five_prime_UTR
ACCTTGAAGAAGAATCGCGA+AGG 0.632979 7_1:-61451158 None:intergenic
CGTCGTTGTGGCTGACAGAG+AGG 0.644565 7_1:-61451063 None:intergenic
AGGAGCGAAGATCACCGAGA+GGG 0.649648 7_1:+61454572 Msa1028500:exon
GAGCAGCATTCTGTTCTCTG+TGG 0.655140 7_1:+61454504 Msa1028500:intron
GCGAGCTGAAGTGCGAACCA+CGG 0.659935 7_1:-61451385 None:intergenic
CCAACCTTCCCAAAATGATG+AGG 0.665843 7_1:-61452595 None:intergenic
GAGGGTGAGATTGAAATTGG+GGG 0.671631 7_1:-61450951 None:intergenic
TAGGAGCGAAGATCACCGAG+AGG 0.719201 7_1:+61454571 Msa1028500:exon

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATATATTTTTTTTTGTTAAG+AGG + chr7_1:61453034-61453053 Msa1028500:intron 10.0%
!! AAACTAATTATCATAATATT+CGG - chr7_1:61454617-61454636 None:intergenic 10.0%
!! AGAAATTACTTGATATATAA+AGG + chr7_1:61451472-61451491 Msa1028500:intron 15.0%
!! GAAATTACTTGATATATAAA+GGG + chr7_1:61451473-61451492 Msa1028500:intron 15.0%
!! AAATTACTTGATATATAAAG+GGG + chr7_1:61451474-61451493 Msa1028500:intron 15.0%
!! AATATATGCTGATATAAAAA+TGG - chr7_1:61451631-61451650 None:intergenic 15.0%
!!! TTTTGATAATACCATTAATA+AGG + chr7_1:61451754-61451773 Msa1028500:intron 15.0%
!!! TTTGATAATACCATTAATAA+GGG + chr7_1:61451755-61451774 Msa1028500:intron 15.0%
!!! TTTTATAAAATCGACTTATA+AGG - chr7_1:61452164-61452183 None:intergenic 15.0%
!! ACAAATATTATAATGATATG+TGG + chr7_1:61454715-61454734 Msa1028500:three_prime_UTR 15.0%
!!! GATTTTTGTTAAATGTTGTT+TGG + chr7_1:61451496-61451515 Msa1028500:intron 20.0%
!!! GAGTCTTTTTTTTTTTTTTG+AGG - chr7_1:61451554-61451573 None:intergenic 20.0%
!! CATATATTAAATTGAATGAC+AGG + chr7_1:61451643-61451662 Msa1028500:intron 20.0%
!!! TTGATAATACCATTAATAAG+GGG + chr7_1:61451756-61451775 Msa1028500:intron 20.0%
!!! ATTATTAGCTTATCTTACAA+TGG + chr7_1:61452356-61452375 Msa1028500:intron 20.0%
!!! ATTTTTTTTTGTTAAGAGGT+AGG + chr7_1:61453038-61453057 Msa1028500:intron 20.0%
!! AACTAAGCTATAAAATGAAA+AGG - chr7_1:61453145-61453164 None:intergenic 20.0%
!!! ATATCTCTTAACTTTCTTTT+TGG - chr7_1:61453175-61453194 None:intergenic 20.0%
!! AAAAAGAAAGTTAAGAGATA+TGG + chr7_1:61453174-61453193 Msa1028500:intron 20.0%
!! GAATTAATTATAGTTAGTGT+CGG - chr7_1:61453369-61453388 None:intergenic 20.0%
!!! AACTCTTAAGTTTTTAACAT+AGG + chr7_1:61453674-61453693 Msa1028500:intron 20.0%
!!! ACTCTTAAGTTTTTAACATA+GGG + chr7_1:61453675-61453694 Msa1028500:intron 20.0%
!! GATCAATATAGATATAGATT+TGG + chr7_1:61453785-61453804 Msa1028500:intron 20.0%
!! ATTATACTTAGCTTATGTAT+AGG - chr7_1:61453818-61453837 None:intergenic 20.0%
!! ATAATTGTATAAATGCTGTA+AGG + chr7_1:61453833-61453852 Msa1028500:intron 20.0%
!! TCAACATCTATATTAATCTT+TGG - chr7_1:61454216-61454235 None:intergenic 20.0%
! GCAAGAATGAATAAGAAAAA+AGG - chr7_1:61451200-61451219 None:intergenic 25.0%
! TTCATTTGAATCTATCCTAT+TGG + chr7_1:61451435-61451454 Msa1028500:intron 25.0%
! GTTATTGGTATAGAAAGAAT+CGG + chr7_1:61451590-61451609 Msa1028500:intron 25.0%
! TTATTGGTATAGAAAGAATC+GGG + chr7_1:61451591-61451610 Msa1028500:intron 25.0%
! TGCATATAAACACTTCTATT+AGG - chr7_1:61451787-61451806 None:intergenic 25.0%
! TAATAATATCCACAAAGTTG+CGG + chr7_1:61452252-61452271 Msa1028500:intron 25.0%
! TGAATGGAAAGAAAAATATG+TGG - chr7_1:61452776-61452795 None:intergenic 25.0%
!! GTAATTGTTGTTCATATGAT+TGG + chr7_1:61452854-61452873 Msa1028500:intron 25.0%
! TCAATGGTCAATTTGTATAT+AGG - chr7_1:61452946-61452965 None:intergenic 25.0%
! ATAGCTGATTGAAATTTGAA+GGG - chr7_1:61453002-61453021 None:intergenic 25.0%
! TATAGCTGATTGAAATTTGA+AGG - chr7_1:61453003-61453022 None:intergenic 25.0%
!! GAATATTTGTTACACTGAAT+TGG + chr7_1:61453073-61453092 Msa1028500:intron 25.0%
! ATCATAATAGAAGACATGTT+AGG + chr7_1:61453340-61453359 Msa1028500:intron 25.0%
! CCAAAATCTACTCTTAAATT+GGG - chr7_1:61453465-61453484 None:intergenic 25.0%
! TCCAAAATCTACTCTTAAAT+TGG - chr7_1:61453466-61453485 None:intergenic 25.0%
! CTGATGGAAATGTTATATAA+TGG + chr7_1:61453621-61453640 Msa1028500:intron 25.0%
!! GGAAATGTTATATAATGGAA+AGG + chr7_1:61453626-61453645 Msa1028500:intron 25.0%
! TCTATGTCTTTATGTTTATG+TGG - chr7_1:61453722-61453741 None:intergenic 25.0%
! TTTGTAACAAAGCTTAAATC+TGG - chr7_1:61453899-61453918 None:intergenic 25.0%
!! GCTTTGTTACAAACAATATA+TGG + chr7_1:61453906-61453925 Msa1028500:intron 25.0%
!!! TGGATGATTGTTTTTTTTCA+AGG + chr7_1:61454100-61454119 Msa1028500:intron 25.0%
!!! TACTATTTAGCTTTTTCTGA+AGG - chr7_1:61454411-61454430 None:intergenic 25.0%
!!! TTCTTAGTTTTCTAGATTGT+AGG + chr7_1:61454642-61454661 Msa1028500:three_prime_UTR 25.0%
ATGTTGACAGTTGAAATTGA+GGG - chr7_1:61450915-61450934 None:intergenic 30.0%
TCGTTAATCTGAGAAAACTT+TGG + chr7_1:61451016-61451035 Msa1028500:five_prime_UTR 30.0%
TCTATCCTATTGGTATAGAA+AGG + chr7_1:61451445-61451464 Msa1028500:intron 30.0%
TTGATCCTTTCTATACCAAT+AGG - chr7_1:61451453-61451472 None:intergenic 30.0%
TGTTTGGATTATTTGCTCAT+AGG + chr7_1:61451512-61451531 Msa1028500:intron 30.0%
AAAAAGACTCTTGTATTGTG+TGG + chr7_1:61451564-61451583 Msa1028500:intron 30.0%
! TGTATTGTGTGGATTGTTAT+TGG + chr7_1:61451575-61451594 Msa1028500:intron 30.0%
TATAAAAATGGCTCCCAAAA+TGG - chr7_1:61451619-61451638 None:intergenic 30.0%
AACAGATTTACCGTTATAAG+CGG - chr7_1:61451719-61451738 None:intergenic 30.0%
!! AGAAGTGTTTATATGCATGT+AGG + chr7_1:61451790-61451809 Msa1028500:intron 30.0%
TGGAATTGCTTTCTTTCTTA+AGG + chr7_1:61451850-61451869 Msa1028500:five_prime_UTR 30.0%
! AACTTTGCTGTATTATTCCA+TGG + chr7_1:61451939-61451958 Msa1028500:five_prime_UTR 30.0%
! TTGGAAAAAAGCACACTTAA+TGG + chr7_1:61452010-61452029 Msa1028500:intron 30.0%
!! AAGCACACTTAATGGTTTTA+TGG + chr7_1:61452018-61452037 Msa1028500:intron 30.0%
CTTATTAACAATTGTGCTCA+TGG + chr7_1:61452057-61452076 Msa1028500:intron 30.0%
TGATATCAAAACCGAATACA+AGG + chr7_1:61452196-61452215 Msa1028500:intron 30.0%
AGTATGTAACAAACAATCGT+TGG + chr7_1:61452393-61452412 Msa1028500:intron 30.0%
!!! GGTTGTTTTTTGGAATTTTG+AGG + chr7_1:61452525-61452544 Msa1028500:five_prime_UTR 30.0%
!!! TTTTTTGGAATTTTGAGGCA+GGG + chr7_1:61452530-61452549 Msa1028500:five_prime_UTR 30.0%
TATGTGGTTAACCTTAGTAA+CGG - chr7_1:61452760-61452779 None:intergenic 30.0%
TGAACAATAGAGGAATTGAA+TGG - chr7_1:61452792-61452811 None:intergenic 30.0%
!! CAAGCTATTTTGAACAATAG+AGG - chr7_1:61452802-61452821 None:intergenic 30.0%
! GTTTGTTGATATTGCAGTTT+AGG + chr7_1:61452888-61452907 Msa1028500:intron 30.0%
TAGACTTAATACACTGAAAG+TGG - chr7_1:61453270-61453289 None:intergenic 30.0%
ACTTTCAGTGTATTAAGTCT+AGG + chr7_1:61453269-61453288 Msa1028500:intron 30.0%
TGTAGTAAGATTTATGCAAC+TGG - chr7_1:61453300-61453319 None:intergenic 30.0%
! CCCAATTTAAGAGTAGATTT+TGG + chr7_1:61453462-61453481 Msa1028500:intron 30.0%
AAATCTCTGATAGCAAGATA+AGG - chr7_1:61453511-61453530 None:intergenic 30.0%
ACTAGCAACATTATATCACA+GGG - chr7_1:61453545-61453564 None:intergenic 30.0%
AACTAGCAACATTATATCAC+AGG - chr7_1:61453546-61453565 None:intergenic 30.0%
ATATATGGCTGTTAGAAAAC+TGG + chr7_1:61453921-61453940 Msa1028500:intron 30.0%
TCAGTGTACAATATTCAGTA+TGG - chr7_1:61453995-61454014 None:intergenic 30.0%
! GAGTGTTTGAATTTCTGTTT+TGG + chr7_1:61454059-61454078 Msa1028500:intron 30.0%
CGCAAAGAAAAGAAATACAA+GGG - chr7_1:61454145-61454164 None:intergenic 30.0%
TCGCAAAGAAAAGAAATACA+AGG - chr7_1:61454146-61454165 None:intergenic 30.0%
!! ATAGATGTTGAGTGTTTTGA+AGG + chr7_1:61454225-61454244 Msa1028500:intron 30.0%
TATAAAGACATCACCACTAA+CGG + chr7_1:61454323-61454342 Msa1028500:five_prime_UTR 30.0%
CTTTCTCAATTTCATTCCCA+CGG + chr7_1:61450765-61450784 Msa1028500:five_prime_UTR 35.0%
!! AGATTGAAATGGAGGCATTA+GGG - chr7_1:61450806-61450825 None:intergenic 35.0%
GATAGAGAGTGAGATTGAAA+TGG - chr7_1:61450817-61450836 None:intergenic 35.0%
TTGAAATTGGAGGTGAGATT+GGG - chr7_1:61450856-61450875 None:intergenic 35.0%
ATTGAAATTGGAGGTGAGAT+TGG - chr7_1:61450857-61450876 None:intergenic 35.0%
ATTGAGGGTGAGATTGAAAT+TGG - chr7_1:61450869-61450888 None:intergenic 35.0%
ATGTTGAGGGTTGAAATTGA+GGG - chr7_1:61450884-61450903 None:intergenic 35.0%
CATGTTGACAGTTGAAATTG+AGG - chr7_1:61450916-61450935 None:intergenic 35.0%
! AGATTGAAATTGGGGGTTTT+GGG - chr7_1:61450947-61450966 None:intergenic 35.0%
TTGAGGGTGAGATTGAAATT+GGG - chr7_1:61450868-61450887 None:intergenic 35.0%
! AGTTTTGCGTTCTCAGTTTT+GGG + chr7_1:61451336-61451355 Msa1028500:five_prime_UTR 35.0%
TTACCTTGAGAAAGTGAAGT+AGG - chr7_1:61451410-61451429 None:intergenic 35.0%
! ACAGGTTCCATTCACTATTT+TGG + chr7_1:61451661-61451680 Msa1028500:intron 35.0%
! CAGGTTCCATTCACTATTTT+GGG + chr7_1:61451662-61451681 Msa1028500:intron 35.0%
TCCAAACTTATCCAGCAAAA+TGG - chr7_1:61451690-61451709 None:intergenic 35.0%
TAGGCATTTCCCCTTATTAA+TGG - chr7_1:61451768-61451787 None:intergenic 35.0%
TCAACAAGTGGAACATTTGT+AGG + chr7_1:61451815-61451834 Msa1028500:five_prime_UTR 35.0%
!! TTTGTAGGCTGTTTTATGAC+TGG + chr7_1:61451830-61451849 Msa1028500:five_prime_UTR 35.0%
TGCTTGGGTAATAATCTATG+AGG + chr7_1:61451977-61451996 Msa1028500:five_prime_UTR 35.0%
! TCTATGAGGTATGTGTCATT+TGG + chr7_1:61451991-61452010 Msa1028500:intron 35.0%
TTAACAATTGTGCTCATGGA+AGG + chr7_1:61452061-61452080 Msa1028500:intron 35.0%
!!! GCTTATTTTTTGCTTGGTAG+AGG + chr7_1:61452432-61452451 Msa1028500:intron 35.0%
!!! GTTTTTTGGAATTTTGAGGC+AGG + chr7_1:61452529-61452548 Msa1028500:five_prime_UTR 35.0%
!!! TTATGCTTTTGGTCTTATGC+TGG + chr7_1:61452553-61452572 Msa1028500:five_prime_UTR 35.0%
GATGGAGAAGAAAAATCGAA+TGG + chr7_1:61452664-61452683 Msa1028500:five_prime_UTR 35.0%
TACGTGGTTAACCTTAGTAA+CGG - chr7_1:61452714-61452733 None:intergenic 35.0%
!! ATAGCTTGCAATAGCTTTGT+AGG + chr7_1:61452814-61452833 Msa1028500:intron 35.0%
TGTTGTTCATATGATTGGTG+TGG + chr7_1:61452859-61452878 Msa1028500:intron 35.0%
GTTGTTCATATGATTGGTGT+GGG + chr7_1:61452860-61452879 Msa1028500:intron 35.0%
TTGTTCATATGATTGGTGTG+GGG + chr7_1:61452861-61452880 Msa1028500:intron 35.0%
!! ATTGACCATTGATTCACGAT+TGG + chr7_1:61452954-61452973 Msa1028500:intron 35.0%
! TGGAGGAACCTTATCTTTTA+TGG + chr7_1:61452974-61452993 Msa1028500:intron 35.0%
GAAGGGAACCATAAAAGATA+AGG - chr7_1:61452985-61453004 None:intergenic 35.0%
TAAACACTAGTTGCTGATCA+AGG + chr7_1:61453218-61453237 Msa1028500:intron 35.0%
AAATCTTACTACACGTGTGA+AGG + chr7_1:61453307-61453326 Msa1028500:intron 35.0%
CTATGTAAGTGAACACAATG+TGG - chr7_1:61453593-61453612 None:intergenic 35.0%
TTACATAGCTCTCAATCTGA+TGG + chr7_1:61453605-61453624 Msa1028500:intron 35.0%
GTTACATAGAGATGTCACAT+AGG + chr7_1:61453648-61453667 Msa1028500:intron 35.0%
! CTGATGTATGTTTTTCTGCA+CGG + chr7_1:61454011-61454030 Msa1028500:intron 35.0%
!!! ACCTGCATATTTTGGTGTTA+GGG + chr7_1:61454037-61454056 Msa1028500:intron 35.0%
GGCAGTGTTTATGATTGTTA+TGG + chr7_1:61454080-61454099 Msa1028500:intron 35.0%
! TTTCTTTTCTTTGCGAGAGT+TGG + chr7_1:61454150-61454169 Msa1028500:intron 35.0%
!!! ATGTTGAGTGTTTTGAAGGA+GGG + chr7_1:61454229-61454248 Msa1028500:intron 35.0%
!! TTTTCTGAAGGTGCACAAAT+AGG - chr7_1:61454399-61454418 None:intergenic 35.0%
AAATTGAGAAAGGTGGAGAG+AGG - chr7_1:61450757-61450776 None:intergenic 40.0%
GGGAATGAAATTGAGAAAGG+TGG - chr7_1:61450764-61450783 None:intergenic 40.0%
CGTGGGAATGAAATTGAGAA+AGG - chr7_1:61450767-61450786 None:intergenic 40.0%
!! GAGATTGAAATGGAGGCATT+AGG - chr7_1:61450807-61450826 None:intergenic 40.0%
AGAGAGTGAGATTGAAATGG+AGG - chr7_1:61450814-61450833 None:intergenic 40.0%
CATGTTGAGGGTTGAAATTG+AGG - chr7_1:61450885-61450904 None:intergenic 40.0%
! GATTGAAATTGGGGGTTTTG+GGG - chr7_1:61450946-61450965 None:intergenic 40.0%
! GAGATTGAAATTGGGGGTTT+TGG - chr7_1:61450948-61450967 None:intergenic 40.0%
TGAGGGTGAGATTGAAATTG+GGG - chr7_1:61450867-61450886 None:intergenic 40.0%
GTTGAGGGTGAGATTGAAAT+TGG - chr7_1:61450957-61450976 None:intergenic 40.0%
CTGTCTCAGATTCATGTTGA+GGG - chr7_1:61450972-61450991 None:intergenic 40.0%
! CAGTTTTGCGTTCTCAGTTT+TGG + chr7_1:61451335-61451354 Msa1028500:five_prime_UTR 40.0%
!! TGGGCAATTTGTTTTCGTCA+AGG + chr7_1:61451355-61451374 Msa1028500:five_prime_UTR 40.0%
!! AAAGAATCGGGAGCCATTTT+GGG + chr7_1:61451603-61451622 Msa1028500:intron 40.0%
ATGGCTCCCAAAATAGTGAA+TGG - chr7_1:61451671-61451690 None:intergenic 40.0%
!! TATTTTGGGAGCCATTTTGC+TGG + chr7_1:61451676-61451695 Msa1028500:five_prime_UTR 40.0%
!! GCCATTTTGCTGGATAAGTT+TGG + chr7_1:61451686-61451705 Msa1028500:five_prime_UTR 40.0%
TGGAAGAAGACCGCTTATAA+CGG + chr7_1:61451706-61451725 Msa1028500:five_prime_UTR 40.0%
GCTGTATTATTCCATGGATC+AGG + chr7_1:61451945-61451964 Msa1028500:five_prime_UTR 40.0%
! ATCAGGATCAGAACTTGCTT+GGG + chr7_1:61451962-61451981 Msa1028500:five_prime_UTR 40.0%
! TCTCTTTTCAGTGTGTGACT+TGG - chr7_1:61452100-61452119 None:intergenic 40.0%
AACCGAATACAAGGTAGATG+CGG + chr7_1:61452205-61452224 Msa1028500:intron 40.0%
ATATCCACAAAGTTGCGGAT+AGG + chr7_1:61452257-61452276 Msa1028500:intron 40.0%
!! ACACTTGCAATTCTGAAGTG+AGG - chr7_1:61452315-61452334 None:intergenic 40.0%
AAAAATAAGCCAGCTGCACA+GGG - chr7_1:61452422-61452441 None:intergenic 40.0%
AAAAAATAAGCCAGCTGCAC+AGG - chr7_1:61452423-61452442 None:intergenic 40.0%
!!! CAGCTGGCTTATTTTTTGCT+TGG + chr7_1:61452426-61452445 Msa1028500:intron 40.0%
!!! TGTGTTGCAGGGTTGTTTTT+TGG + chr7_1:61452515-61452534 Msa1028500:intron 40.0%
! ATACCTCAGCCTCATCATTT+TGG + chr7_1:61452586-61452605 Msa1028500:five_prime_UTR 40.0%
! TACCTCAGCCTCATCATTTT+GGG + chr7_1:61452587-61452606 Msa1028500:five_prime_UTR 40.0%
!! CTCATCATTTTGGGAAGGTT+GGG + chr7_1:61452596-61452615 Msa1028500:five_prime_UTR 40.0%
! TAACCTTAGTAACGGAGAAG+AGG - chr7_1:61452706-61452725 None:intergenic 40.0%
GAGTAGCAGCTAAAAATACG+TGG - chr7_1:61452730-61452749 None:intergenic 40.0%
! TAACCTTAGTAACGGAGAAG+AGG - chr7_1:61452706-61452725 None:intergenic 40.0%
GTATATAGGCACACTGCAAA+AGG - chr7_1:61452932-61452951 None:intergenic 40.0%
TTCCTCCAATCGTGAATCAA+TGG - chr7_1:61452962-61452981 None:intergenic 40.0%
!!! GACCTGCATATTTTGGTGTT+AGG + chr7_1:61454036-61454055 Msa1028500:intron 40.0%
TCCCTAACACCAAAATATGC+AGG - chr7_1:61454041-61454060 None:intergenic 40.0%
!!! GATGTTGAGTGTTTTGAAGG+AGG + chr7_1:61454228-61454247 Msa1028500:intron 40.0%
CCATCTCTTTGATCCACTTT+AGG - chr7_1:61454374-61454393 None:intergenic 40.0%
CCTAAAGTGGATCAAAGAGA+TGG + chr7_1:61454371-61454390 Msa1028500:CDS 40.0%
! GCGTCGATGATACAGATTAA+AGG - chr7_1:61454470-61454489 None:intergenic 40.0%
! GTTCCTTTGGTTCCTTATTC+CGG + chr7_1:61454529-61454548 Msa1028500:CDS 40.0%
AAACCGGAATAAGGAACCAA+AGG - chr7_1:61454535-61454554 None:intergenic 40.0%
TTTGTGGAAGAGGAGGAAAT+AGG + chr7_1:61454552-61454571 Msa1028500:CDS 40.0%
TTTGATCTTGATCTCCCTCT+CGG - chr7_1:61454589-61454608 None:intergenic 40.0%
!!! GTTTTGAAGAGTGGAACCGT+GGG - chr7_1:61450784-61450803 None:intergenic 45.0%
!!! GGCATTAGGGTTTTGAAGAG+TGG - chr7_1:61450793-61450812 None:intergenic 45.0%
AATTGGAGGTGAGATTGGGA+GGG - chr7_1:61450852-61450871 None:intergenic 45.0%
AAATTGGAGGTGAGATTGGG+AGG - chr7_1:61450853-61450872 None:intergenic 45.0%
GAGGGTGAGATTGAAATTGG+AGG - chr7_1:61450866-61450885 None:intergenic 45.0%
GAGGGAGAGATTCATGTTGA+GGG - chr7_1:61450897-61450916 None:intergenic 45.0%
TGAGGGAGAGATTCATGTTG+AGG - chr7_1:61450898-61450917 None:intergenic 45.0%
GAGGGTGAGATTGAAATTGG+GGG - chr7_1:61450866-61450885 None:intergenic 45.0%
GCTGTCTCAGATTCATGTTG+AGG - chr7_1:61450973-61450992 None:intergenic 45.0%
GTAACGAAGTAACGTCGTTG+TGG - chr7_1:61451078-61451097 None:intergenic 45.0%
GTTACTTCGTTACTTCGCGA+TGG + chr7_1:61451085-61451104 Msa1028500:five_prime_UTR 45.0%
TTACTTCGCGATGGTGTGTT+TGG + chr7_1:61451094-61451113 Msa1028500:five_prime_UTR 45.0%
GAAGAATCGCGAAGGAATGA+AGG - chr7_1:61451153-61451172 None:intergenic 45.0%
TCCTTCGCGATTCTTCTTCA+AGG + chr7_1:61451157-61451176 Msa1028500:five_prime_UTR 45.0%
ACCTTGAAGAAGAATCGCGA+AGG - chr7_1:61451161-61451180 None:intergenic 45.0%
TCGCCTACTTCACTTTCTCA+AGG + chr7_1:61451404-61451423 Msa1028500:five_prime_UTR 45.0%
! GAAAGAATCGGGAGCCATTT+TGG + chr7_1:61451602-61451621 Msa1028500:intron 45.0%
TGCATGTAGGCTTCAACAAG+TGG + chr7_1:61451803-61451822 Msa1028500:intron 45.0%
! CAAGTTCTGATCCTGATCCA+TGG - chr7_1:61451959-61451978 None:intergenic 45.0%
! GATCAGGATCAGAACTTGCT+TGG + chr7_1:61451961-61451980 Msa1028500:five_prime_UTR 45.0%
CACCGCATCTACCTTGTATT+CGG - chr7_1:61452210-61452229 None:intergenic 45.0%
!! GTAGATGCGGTGTTGATGTA+CGG + chr7_1:61452218-61452237 Msa1028500:intron 45.0%
GAAACCTATCCGCAACTTTG+TGG - chr7_1:61452264-61452283 None:intergenic 45.0%
! TTTTGCTTGGTAGAGGCTGT+TGG + chr7_1:61452439-61452458 Msa1028500:intron 45.0%
!!! AGCGTTTGGTTTTGTGTTGC+AGG + chr7_1:61452503-61452522 Msa1028500:intron 45.0%
!!! GCGTTTGGTTTTGTGTTGCA+GGG + chr7_1:61452504-61452523 Msa1028500:intron 45.0%
! TTGAGGCAGGGTTATGCTTT+TGG + chr7_1:61452542-61452561 Msa1028500:five_prime_UTR 45.0%
GAGGCTGAGGTATACACAAA+TGG - chr7_1:61452579-61452598 None:intergenic 45.0%
TTCCCAAAATGATGAGGCTG+AGG - chr7_1:61452592-61452611 None:intergenic 45.0%
!! TCAGCCTCATCATTTTGGGA+AGG + chr7_1:61452591-61452610 Msa1028500:five_prime_UTR 45.0%
CCAACCTTCCCAAAATGATG+AGG - chr7_1:61452598-61452617 None:intergenic 45.0%
!! CCTCATCATTTTGGGAAGGT+TGG + chr7_1:61452595-61452614 Msa1028500:five_prime_UTR 45.0%
CAGTCACGTTCACAAAGAAG+AGG - chr7_1:61452641-61452660 None:intergenic 45.0%
ACTCCTCTTCTCCGTTACTA+AGG + chr7_1:61452700-61452719 Msa1028500:five_prime_UTR 45.0%
ACTCCTCTTCTCCGTTACTA+AGG + chr7_1:61452700-61452719 Msa1028500:five_prime_UTR 45.0%
CATATGATTGGTGTGGGGTT+TGG + chr7_1:61452866-61452885 Msa1028500:intron 45.0%
!! GACCATTGATTCACGATTGG+AGG + chr7_1:61452957-61452976 Msa1028500:intron 45.0%
CAGAAGCAATGGGATTGAGA+TGG + chr7_1:61454346-61454365 Msa1028500:exon 45.0%
GATTGAGATGGTGCCTAAAG+TGG + chr7_1:61454358-61454377 Msa1028500:CDS 45.0%
! TGGTTCCTTATTCCGGTTTG+TGG + chr7_1:61454536-61454555 Msa1028500:CDS 45.0%
CTCTTCCACAAACCGGAATA+AGG - chr7_1:61454544-61454563 None:intergenic 45.0%
! CTTATTCCGGTTTGTGGAAG+AGG + chr7_1:61454542-61454561 Msa1028500:CDS 45.0%
TTTCCTCCTCTTCCACAAAC+CGG - chr7_1:61454551-61454570 None:intergenic 45.0%
!!! GGTTTTGAAGAGTGGAACCG+TGG - chr7_1:61450785-61450804 None:intergenic 50.0%
GAAGTGAGGTGAAGAAGTCG+CGG - chr7_1:61452301-61452320 None:intergenic 50.0%
AAGGTTGGGCTGCTTGTGTT+TGG + chr7_1:61452610-61452629 Msa1028500:five_prime_UTR 50.0%
TTGTGAACGTGACTGCTCGA+TGG + chr7_1:61452646-61452665 Msa1028500:five_prime_UTR 50.0%
! CACGGCTGACCTGCATATTT+TGG + chr7_1:61454029-61454048 Msa1028500:intron 50.0%
!! TTGAAGGAGGGTCGACTTGT+TGG + chr7_1:61454241-61454260 Msa1028500:intron 50.0%
!! TGAAGGAGGGTCGACTTGTT+GGG + chr7_1:61454242-61454261 Msa1028500:intron 50.0%
ACCACTAACGGCAGAAGCAA+TGG + chr7_1:61454335-61454354 Msa1028500:exon 50.0%
CCACTAACGGCAGAAGCAAT+GGG + chr7_1:61454336-61454355 Msa1028500:exon 50.0%
GAGCAGCATTCTGTTCTCTG+TGG + chr7_1:61454504-61454523 Msa1028500:intron 50.0%
GTTCTCTGTGGCTGTTCCTT+TGG + chr7_1:61454516-61454535 Msa1028500:CDS 50.0%
! ATTCCGGTTTGTGGAAGAGG+AGG + chr7_1:61454545-61454564 Msa1028500:CDS 50.0%
!! GGTGAGATTGGGAGGGTGTT+AGG - chr7_1:61450845-61450864 None:intergenic 55.0%
ACCACGTGCAGAAGCAGGTT+GGG - chr7_1:61451129-61451148 None:intergenic 55.0%
AACCACGTGCAGAAGCAGGT+TGG - chr7_1:61451130-61451149 None:intergenic 55.0%
AAGGAACCACGTGCAGAAGC+AGG - chr7_1:61451134-61451153 None:intergenic 55.0%
! TTCGTCAAGGCATTTCGCCG+TGG + chr7_1:61451368-61451387 Msa1028500:five_prime_UTR 55.0%
!!! GGCAGTACTGCTGCTGGTTT+TGG + chr7_1:61452460-61452479 Msa1028500:intron 55.0%
TTGCTGCTGACAGCAGCGTT+TGG + chr7_1:61452489-61452508 Msa1028500:intron 55.0%
!! GAAGGAGGGTCGACTTGTTG+GGG + chr7_1:61454243-61454262 Msa1028500:intron 55.0%
CCCATTGCTTCTGCCGTTAG+TGG - chr7_1:61454339-61454358 None:intergenic 55.0%
TAGGAGCGAAGATCACCGAG+AGG + chr7_1:61454571-61454590 Msa1028500:exon 55.0%
AGGAGCGAAGATCACCGAGA+GGG + chr7_1:61454572-61454591 Msa1028500:exon 55.0%
CGTCGTTGTGGCTGACAGAG+AGG - chr7_1:61451066-61451085 None:intergenic 60.0%
ACCCAACCTGCTTCTGCACG+TGG + chr7_1:61451125-61451144 Msa1028500:five_prime_UTR 60.0%
GCGAGCTGAAGTGCGAACCA+CGG - chr7_1:61451388-61451407 None:intergenic 60.0%
GCTGTTGGCAGTACTGCTGC+TGG + chr7_1:61452454-61452473 Msa1028500:intron 60.0%
!! AGGGTCGACTTGTTGGGGAC+TGG + chr7_1:61454248-61454267 Msa1028500:five_prime_UTR 60.0%
!! GTCGACTTGTTGGGGACTGG+TGG + chr7_1:61454251-61454270 Msa1028500:five_prime_UTR 60.0%
CTGGTGGCTCGCAACTGTGA+AGG + chr7_1:61454267-61454286 Msa1028500:five_prime_UTR 60.0%
! CGTTGGTGTCCCTGTGCAGC+TGG + chr7_1:61452410-61452429 Msa1028500:intron 65.0%
Chromosome Type Strat End Strand Name
chr7_1 gene 61450747 61454752 61450747 ID=Msa1028500;Name=Msa1028500
chr7_1 mRNA 61450747 61454752 61450747 ID=Msa1028500-mRNA-1;Parent=Msa1028500;Name=Msa1028500-mRNA-1;_AED=0.30;_eAED=0.30;_QI=1279|1|1|1|0.4|0.5|6|179|45
chr7_1 exon 61450747 61451425 61450747 ID=Msa1028500-mRNA-1:exon:12942;Parent=Msa1028500-mRNA-1
chr7_1 exon 61451665 61451727 61451665 ID=Msa1028500-mRNA-1:exon:12943;Parent=Msa1028500-mRNA-1
chr7_1 exon 61451812 61451998 61451812 ID=Msa1028500-mRNA-1:exon:12944;Parent=Msa1028500-mRNA-1
chr7_1 exon 61452525 61452767 61452525 ID=Msa1028500-mRNA-1:exon:12945;Parent=Msa1028500-mRNA-1
chr7_1 exon 61454247 61454427 61454247 ID=Msa1028500-mRNA-1:exon:12946;Parent=Msa1028500-mRNA-1
chr7_1 exon 61454510 61454752 61454510 ID=Msa1028500-mRNA-1:exon:12947;Parent=Msa1028500-mRNA-1
chr7_1 five_prime_UTR 61450747 61451425 61450747 ID=Msa1028500-mRNA-1:five_prime_utr;Parent=Msa1028500-mRNA-1
chr7_1 five_prime_UTR 61451665 61451727 61451665 ID=Msa1028500-mRNA-1:five_prime_utr;Parent=Msa1028500-mRNA-1
chr7_1 five_prime_UTR 61451812 61451998 61451812 ID=Msa1028500-mRNA-1:five_prime_utr;Parent=Msa1028500-mRNA-1
chr7_1 five_prime_UTR 61452525 61452767 61452525 ID=Msa1028500-mRNA-1:five_prime_utr;Parent=Msa1028500-mRNA-1
chr7_1 five_prime_UTR 61454247 61454353 61454247 ID=Msa1028500-mRNA-1:five_prime_utr;Parent=Msa1028500-mRNA-1
chr7_1 CDS 61454354 61454427 61454354 ID=Msa1028500-mRNA-1:cds;Parent=Msa1028500-mRNA-1
chr7_1 CDS 61454510 61454573 61454510 ID=Msa1028500-mRNA-1:cds;Parent=Msa1028500-mRNA-1
chr7_1 three_prime_UTR 61454574 61454752 61454574 ID=Msa1028500-mRNA-1:three_prime_utr;Parent=Msa1028500-mRNA-1
Gene Sequence

>Msa1028500

ATGGGATTGAGATGGTGCCTAAAGTGGATCAAAGAGATGGTGCCTATTTGTGCACCTTCAGAAAAAGCTAAATACATTCTGTTCTCTGTGGCTGTTCCTTTGGTTCCTTATTCCGGTTTGTGGAAGAGGAGGAAATAG
Protein sequence

>Msa1028500

MGLRWCLKWIKEMVPICAPSEKAKYILFSVAVPLVPYSGLWKRRK*