AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0298110

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 65 sgRNAs with CRISPR-Local

Find 284 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACAGGTTCCATTCACTATTT+TGG 0.089427 2_3:-52014989 Msa0298110:intron
ATACCTCAGCCTCATCATTT+TGG 0.183295 2_3:-52014064 Msa0298110:CDS
GTTGAGGGTGAGATTGAAAT+TGG 0.243871 2_3:+52015695 None:intergenic
TTGAGGCAGGGTTATGCTTT+TGG 0.248771 2_3:-52014108 Msa0298110:CDS
GGTGAGATTGGGAGGGTGTT+AGG 0.259018 2_3:+52015807 None:intergenic
ATTGAGGGTGAGATTGAAAT+TGG 0.287143 2_3:+52015783 None:intergenic
GGCAGTACTGCTGCTGGTTT+TGG 0.304093 2_3:-52014190 Msa0298110:CDS
TCCAAACTTATCCAGCAAAA+TGG 0.312997 2_3:+52014963 None:intergenic
TGGAATTGCTTTCTTTCTTA+AGG 0.316427 2_3:-52014800 Msa0298110:intron
GAGATTGAAATGGAGGCATT+AGG 0.323129 2_3:+52015845 None:intergenic
TCCTTCGCGATTCTTCTTCA+AGG 0.341884 2_3:-52015492 Msa0298110:five_prime_UTR
TCAACAAGTGGAACATTTGT+AGG 0.364179 2_3:-52014835 Msa0298110:five_prime_UTR
AAGGTTGGGCTGCTTGTGTT+TGG 0.367926 2_3:-52014040 Msa0298110:CDS
TTACTTCGCGATGGTGTGTT+TGG 0.372301 2_3:-52015555 Msa0298110:five_prime_UTR
AGATTGAAATGGAGGCATTA+GGG 0.380645 2_3:+52015846 None:intergenic
ATTGAAATTGGAGGTGAGAT+TGG 0.389915 2_3:+52015795 None:intergenic
GATAGAGAGTGAGATTGAAA+TGG 0.408383 2_3:+52015835 None:intergenic
TTGAAATTGGAGGTGAGATT+GGG 0.411999 2_3:+52015796 None:intergenic
TTGAGGGTGAGATTGAAATT+GGG 0.413088 2_3:+52015696 None:intergenic
ACCACGTGCAGAAGCAGGTT+GGG 0.433448 2_3:+52015523 None:intergenic
GATCAGGATCAGAACTTGCT+TGG 0.450555 2_3:-52014689 Msa0298110:intron
GAGGGAGAGATTCATGTTGA+GGG 0.470478 2_3:+52015755 None:intergenic
CATGTTGAGGGTTGAAATTG+AGG 0.471754 2_3:+52015767 None:intergenic
TTGCTGCTGACAGCAGCGTT+TGG 0.480122 2_3:-52014161 Msa0298110:CDS
TGAGGGAGAGATTCATGTTG+AGG 0.484884 2_3:+52015754 None:intergenic
AGAGAGTGAGATTGAAATGG+AGG 0.485227 2_3:+52015838 None:intergenic
GAAGAATCGCGAAGGAATGA+AGG 0.495389 2_3:+52015499 None:intergenic
TTACCTTGAGAAAGTGAAGT+AGG 0.497019 2_3:+52015243 None:intergenic
ATGGCTCCCAAAATAGTGAA+TGG 0.498993 2_3:+52014982 None:intergenic
TCGTTAATCTGAGAAAACTT+TGG 0.501340 2_3:-52015633 Msa0298110:five_prime_UTR
AAGGAACCACGTGCAGAAGC+AGG 0.503698 2_3:+52015518 None:intergenic
CTGTCTCAGATTCATGTTGA+GGG 0.504404 2_3:+52015680 None:intergenic
TGAGGGTGAGATTGAAATTG+GGG 0.506801 2_3:+52015697 None:intergenic
ATCAGGATCAGAACTTGCTT+GGG 0.507458 2_3:-52014688 Msa0298110:intron
CTTTCTCAATTTCATTCCCA+CGG 0.511358 2_3:-52015884 Msa0298110:five_prime_UTR
TGCTTGGGTAATAATCTATG+AGG 0.516623 2_3:-52014673 Msa0298110:intron
CATGTTGACAGTTGAAATTG+AGG 0.518206 2_3:+52015736 None:intergenic
GAGGCTGAGGTATACACAAA+TGG 0.531607 2_3:+52014074 None:intergenic
GCTGTCGGCAGTACTGCTGC+TGG 0.537550 2_3:-52014196 Msa0298110:CDS
TGGAAGAAGACCGCTTATAA+CGG 0.539392 2_3:-52014944 Msa0298110:intron
TTCCCAAAATGATGAGGCTG+AGG 0.560951 2_3:+52014061 None:intergenic
AAATTGGAGGTGAGATTGGG+AGG 0.564532 2_3:+52015799 None:intergenic
AACAGATTTACCGTTATAAG+CGG 0.575668 2_3:+52014934 None:intergenic
CGTGGGAATGAAATTGAGAA+AGG 0.577048 2_3:+52015885 None:intergenic
ATGTTGACAGTTGAAATTGA+GGG 0.578438 2_3:+52015737 None:intergenic
ATGTTGAGGGTTGAAATTGA+GGG 0.579664 2_3:+52015768 None:intergenic
TTGTGAACGTGACTGCTCGA+TGG 0.580508 2_3:-52014004 Msa0298110:CDS
TGCATGTAGGCTTCAACAAG+TGG 0.583443 2_3:-52014847 Msa0298110:intron
AAATTGAGAAAGGTGGAGAG+AGG 0.585921 2_3:+52015895 None:intergenic
ACCCAACCTGCTTCTGCACG+TGG 0.587590 2_3:-52015524 Msa0298110:five_prime_UTR
TCGCCTACTTCACTTTCTCA+AGG 0.591636 2_3:-52015246 Msa0298110:intron
CAGTCACGTTCACAAAGAAG+AGG 0.597794 2_3:+52014012 None:intergenic
AATTGGAGGTGAGATTGGGA+GGG 0.602277 2_3:+52015800 None:intergenic
AACCACGTGCAGAAGCAGGT+TGG 0.602872 2_3:+52015522 None:intergenic
GAGGGTGAGATTGAAATTGG+AGG 0.608950 2_3:+52015786 None:intergenic
GGGAATGAAATTGAGAAAGG+TGG 0.612757 2_3:+52015888 None:intergenic
GCTGTCTCAGATTCATGTTG+AGG 0.617374 2_3:+52015679 None:intergenic
TTCGTCAAGGCATTTCGCCG+TGG 0.619990 2_3:-52015282 Msa0298110:five_prime_UTR
GTAACGAAGTAACGTCGTTG+TGG 0.624471 2_3:+52015574 None:intergenic
GTTACTTCGTTACTTCGCGA+TGG 0.627642 2_3:-52015564 Msa0298110:five_prime_UTR
ACCTTGAAGAAGAATCGCGA+AGG 0.632979 2_3:+52015491 None:intergenic
CGTCGTTGTGGCTGACAGAG+AGG 0.644565 2_3:+52015586 None:intergenic
GCGAGCTGAAGTGCGAACCA+CGG 0.659935 2_3:+52015265 None:intergenic
CCAACCTTCCCAAAATGATG+AGG 0.665843 2_3:+52014055 None:intergenic
GAGGGTGAGATTGAAATTGG+GGG 0.671631 2_3:+52015698 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGAAATTACTTGATATATAA+AGG - chr2_3:52014702-52014721 Msa0298110:intron 15.0%
!! GAAATTACTTGATATATAAA+GGG - chr2_3:52014703-52014722 Msa0298110:intron 15.0%
!! AAATTACTTGATATATAAAG+GGG - chr2_3:52014704-52014723 Msa0298110:intron 15.0%
!! AATATATGCTGATATAAAAA+TGG + chr2_3:52014861-52014880 None:intergenic 15.0%
!!! TTTTGATAATACCATTAATA+AGG - chr2_3:52014984-52015003 Msa0298110:five_prime_UTR 15.0%
!!! TTTGATAATACCATTAATAA+GGG - chr2_3:52014985-52015004 Msa0298110:five_prime_UTR 15.0%
!!! TTTTATAAAATCGACTTATA+AGG + chr2_3:52015394-52015413 None:intergenic 15.0%
!! AGAAATTACTTGATATATAA+AGG - chr2_3:52014702-52014721 Msa0298110:intron 15.0%
!! GAAATTACTTGATATATAAA+GGG - chr2_3:52014703-52014722 Msa0298110:intron 15.0%
!! AAATTACTTGATATATAAAG+GGG - chr2_3:52014704-52014723 Msa0298110:intron 15.0%
!! AATATATGCTGATATAAAAA+TGG + chr2_3:52014861-52014880 None:intergenic 15.0%
!!! TTTTGATAATACCATTAATA+AGG - chr2_3:52014984-52015003 Msa0298110:five_prime_UTR 15.0%
!!! TTTGATAATACCATTAATAA+GGG - chr2_3:52014985-52015004 Msa0298110:five_prime_UTR 15.0%
!!! TTTTATAAAATCGACTTATA+AGG + chr2_3:52015394-52015413 None:intergenic 15.0%
!!! GATTTTTGTTAAATGTTGTT+TGG - chr2_3:52014726-52014745 Msa0298110:intron 20.0%
!!! GAGTCTTTTTTTTTTTTTTG+AGG + chr2_3:52014784-52014803 None:intergenic 20.0%
!! CATATATTAAATTGAATGAC+AGG - chr2_3:52014873-52014892 Msa0298110:intron 20.0%
!!! TTGATAATACCATTAATAAG+GGG - chr2_3:52014986-52015005 Msa0298110:five_prime_UTR 20.0%
!!! ATTATTAGCTTATCTTACAA+TGG - chr2_3:52015586-52015605 Msa0298110:five_prime_UTR 20.0%
!!! GATTTTTGTTAAATGTTGTT+TGG - chr2_3:52014726-52014745 Msa0298110:intron 20.0%
!!! GAGTCTTTTTTTTTTTTTTG+AGG + chr2_3:52014784-52014803 None:intergenic 20.0%
!! CATATATTAAATTGAATGAC+AGG - chr2_3:52014873-52014892 Msa0298110:intron 20.0%
!!! TTGATAATACCATTAATAAG+GGG - chr2_3:52014986-52015005 Msa0298110:five_prime_UTR 20.0%
!!! ATTATTAGCTTATCTTACAA+TGG - chr2_3:52015586-52015605 Msa0298110:five_prime_UTR 20.0%
! GCAAGAATGAATAAGAAAAA+AGG + chr2_3:52014431-52014450 None:intergenic 25.0%
! TTCATTTGAATCTATCCTAT+TGG - chr2_3:52014665-52014684 Msa0298110:intron 25.0%
! GTTATTGGTATAGAAAGAAT+CGG - chr2_3:52014820-52014839 Msa0298110:five_prime_UTR 25.0%
! TTATTGGTATAGAAAGAATC+GGG - chr2_3:52014821-52014840 Msa0298110:five_prime_UTR 25.0%
! TGCATATAAACACTTCTATT+AGG + chr2_3:52015017-52015036 None:intergenic 25.0%
! TAATAATATCCACAAAGTTG+CGG - chr2_3:52015482-52015501 Msa0298110:five_prime_UTR 25.0%
! GCAAGAATGAATAAGAAAAA+AGG + chr2_3:52014431-52014450 None:intergenic 25.0%
! TTCATTTGAATCTATCCTAT+TGG - chr2_3:52014665-52014684 Msa0298110:intron 25.0%
! GTTATTGGTATAGAAAGAAT+CGG - chr2_3:52014820-52014839 Msa0298110:five_prime_UTR 25.0%
! TTATTGGTATAGAAAGAATC+GGG - chr2_3:52014821-52014840 Msa0298110:five_prime_UTR 25.0%
! TGCATATAAACACTTCTATT+AGG + chr2_3:52015017-52015036 None:intergenic 25.0%
! TAATAATATCCACAAAGTTG+CGG - chr2_3:52015482-52015501 Msa0298110:five_prime_UTR 25.0%
ATGTTGACAGTTGAAATTGA+GGG + chr2_3:52014146-52014165 None:intergenic 30.0%
TCGTTAATCTGAGAAAACTT+TGG - chr2_3:52014247-52014266 Msa0298110:intron 30.0%
TCTATCCTATTGGTATAGAA+AGG - chr2_3:52014675-52014694 Msa0298110:exon 30.0%
TTGATCCTTTCTATACCAAT+AGG + chr2_3:52014683-52014702 None:intergenic 30.0%
TGTTTGGATTATTTGCTCAT+AGG - chr2_3:52014742-52014761 Msa0298110:intron 30.0%
AAAAAGACTCTTGTATTGTG+TGG - chr2_3:52014794-52014813 Msa0298110:intron 30.0%
! TGTATTGTGTGGATTGTTAT+TGG - chr2_3:52014805-52014824 Msa0298110:five_prime_UTR 30.0%
TATAAAAATGGCTCCCAAAA+TGG + chr2_3:52014849-52014868 None:intergenic 30.0%
AACAGATTTACCGTTATAAG+CGG + chr2_3:52014949-52014968 None:intergenic 30.0%
!! AGAAGTGTTTATATGCATGT+AGG - chr2_3:52015020-52015039 Msa0298110:intron 30.0%
TGGAATTGCTTTCTTTCTTA+AGG - chr2_3:52015080-52015099 Msa0298110:intron 30.0%
! AACTTTGCTGTATTATTCCA+TGG - chr2_3:52015169-52015188 Msa0298110:intron 30.0%
! TTGGAAAAAAGCACACTTAA+TGG - chr2_3:52015240-52015259 Msa0298110:intron 30.0%
!! AAGCACACTTAATGGTTTTA+TGG - chr2_3:52015248-52015267 Msa0298110:five_prime_UTR 30.0%
CTTATTAACAATTGTGCTCA+TGG - chr2_3:52015287-52015306 Msa0298110:five_prime_UTR 30.0%
TGATATCAAAACCGAATACA+AGG - chr2_3:52015426-52015445 Msa0298110:five_prime_UTR 30.0%
AGTATGTAACAAACAATCGT+TGG - chr2_3:52015623-52015642 Msa0298110:five_prime_UTR 30.0%
!!! GGTTGTTTTTTGGAATTTTG+AGG - chr2_3:52015755-52015774 Msa0298110:five_prime_UTR 30.0%
!!! TTTTTTGGAATTTTGAGGCA+GGG - chr2_3:52015760-52015779 Msa0298110:five_prime_UTR 30.0%
ATGTTGACAGTTGAAATTGA+GGG + chr2_3:52014146-52014165 None:intergenic 30.0%
TCGTTAATCTGAGAAAACTT+TGG - chr2_3:52014247-52014266 Msa0298110:intron 30.0%
TCTATCCTATTGGTATAGAA+AGG - chr2_3:52014675-52014694 Msa0298110:exon 30.0%
TTGATCCTTTCTATACCAAT+AGG + chr2_3:52014683-52014702 None:intergenic 30.0%
TGTTTGGATTATTTGCTCAT+AGG - chr2_3:52014742-52014761 Msa0298110:intron 30.0%
AAAAAGACTCTTGTATTGTG+TGG - chr2_3:52014794-52014813 Msa0298110:intron 30.0%
! TGTATTGTGTGGATTGTTAT+TGG - chr2_3:52014805-52014824 Msa0298110:five_prime_UTR 30.0%
TATAAAAATGGCTCCCAAAA+TGG + chr2_3:52014849-52014868 None:intergenic 30.0%
AACAGATTTACCGTTATAAG+CGG + chr2_3:52014949-52014968 None:intergenic 30.0%
!! AGAAGTGTTTATATGCATGT+AGG - chr2_3:52015020-52015039 Msa0298110:intron 30.0%
TGGAATTGCTTTCTTTCTTA+AGG - chr2_3:52015080-52015099 Msa0298110:intron 30.0%
! AACTTTGCTGTATTATTCCA+TGG - chr2_3:52015169-52015188 Msa0298110:intron 30.0%
! TTGGAAAAAAGCACACTTAA+TGG - chr2_3:52015240-52015259 Msa0298110:intron 30.0%
!! AAGCACACTTAATGGTTTTA+TGG - chr2_3:52015248-52015267 Msa0298110:five_prime_UTR 30.0%
CTTATTAACAATTGTGCTCA+TGG - chr2_3:52015287-52015306 Msa0298110:five_prime_UTR 30.0%
TGATATCAAAACCGAATACA+AGG - chr2_3:52015426-52015445 Msa0298110:five_prime_UTR 30.0%
AGTATGTAACAAACAATCGT+TGG - chr2_3:52015623-52015642 Msa0298110:five_prime_UTR 30.0%
!!! GGTTGTTTTTTGGAATTTTG+AGG - chr2_3:52015755-52015774 Msa0298110:five_prime_UTR 30.0%
!!! TTTTTTGGAATTTTGAGGCA+GGG - chr2_3:52015760-52015779 Msa0298110:five_prime_UTR 30.0%
CTTTCTCAATTTCATTCCCA+CGG - chr2_3:52013996-52014015 Msa0298110:CDS 35.0%
!! AGATTGAAATGGAGGCATTA+GGG + chr2_3:52014037-52014056 None:intergenic 35.0%
GATAGAGAGTGAGATTGAAA+TGG + chr2_3:52014048-52014067 None:intergenic 35.0%
TTGAAATTGGAGGTGAGATT+GGG + chr2_3:52014087-52014106 None:intergenic 35.0%
ATTGAAATTGGAGGTGAGAT+TGG + chr2_3:52014088-52014107 None:intergenic 35.0%
ATTGAGGGTGAGATTGAAAT+TGG + chr2_3:52014100-52014119 None:intergenic 35.0%
ATGTTGAGGGTTGAAATTGA+GGG + chr2_3:52014115-52014134 None:intergenic 35.0%
CATGTTGACAGTTGAAATTG+AGG + chr2_3:52014147-52014166 None:intergenic 35.0%
! AGATTGAAATTGGGGGTTTT+GGG + chr2_3:52014178-52014197 None:intergenic 35.0%
TTGAGGGTGAGATTGAAATT+GGG + chr2_3:52014099-52014118 None:intergenic 35.0%
! AGTTTTGCGTTCTCAGTTTT+GGG - chr2_3:52014566-52014585 Msa0298110:intron 35.0%
TTACCTTGAGAAAGTGAAGT+AGG + chr2_3:52014640-52014659 None:intergenic 35.0%
! ACAGGTTCCATTCACTATTT+TGG - chr2_3:52014891-52014910 Msa0298110:intron 35.0%
! CAGGTTCCATTCACTATTTT+GGG - chr2_3:52014892-52014911 Msa0298110:intron 35.0%
TCCAAACTTATCCAGCAAAA+TGG + chr2_3:52014920-52014939 None:intergenic 35.0%
TAGGCATTTCCCCTTATTAA+TGG + chr2_3:52014998-52015017 None:intergenic 35.0%
TCAACAAGTGGAACATTTGT+AGG - chr2_3:52015045-52015064 Msa0298110:intron 35.0%
!! TTTGTAGGCTGTTTTATGAC+TGG - chr2_3:52015060-52015079 Msa0298110:intron 35.0%
TGCTTGGGTAATAATCTATG+AGG - chr2_3:52015207-52015226 Msa0298110:intron 35.0%
! TCTATGAGGTATGTGTCATT+TGG - chr2_3:52015221-52015240 Msa0298110:intron 35.0%
TTAACAATTGTGCTCATGGA+AGG - chr2_3:52015291-52015310 Msa0298110:five_prime_UTR 35.0%
!!! GCTTATTTTTTGCTTGGTAG+AGG - chr2_3:52015662-52015681 Msa0298110:five_prime_UTR 35.0%
!!! GTTTTTTGGAATTTTGAGGC+AGG - chr2_3:52015759-52015778 Msa0298110:five_prime_UTR 35.0%
!!! TTATGCTTTTGGTCTTATGC+TGG - chr2_3:52015783-52015802 Msa0298110:five_prime_UTR 35.0%
GATGGAGAAGAAAAATCGAA+TGG - chr2_3:52015894-52015913 Msa0298110:five_prime_UTR 35.0%
CTTTCTCAATTTCATTCCCA+CGG - chr2_3:52013996-52014015 Msa0298110:CDS 35.0%
!! AGATTGAAATGGAGGCATTA+GGG + chr2_3:52014037-52014056 None:intergenic 35.0%
GATAGAGAGTGAGATTGAAA+TGG + chr2_3:52014048-52014067 None:intergenic 35.0%
TTGAAATTGGAGGTGAGATT+GGG + chr2_3:52014087-52014106 None:intergenic 35.0%
ATTGAAATTGGAGGTGAGAT+TGG + chr2_3:52014088-52014107 None:intergenic 35.0%
ATTGAGGGTGAGATTGAAAT+TGG + chr2_3:52014100-52014119 None:intergenic 35.0%
ATGTTGAGGGTTGAAATTGA+GGG + chr2_3:52014115-52014134 None:intergenic 35.0%
CATGTTGACAGTTGAAATTG+AGG + chr2_3:52014147-52014166 None:intergenic 35.0%
! AGATTGAAATTGGGGGTTTT+GGG + chr2_3:52014178-52014197 None:intergenic 35.0%
TTGAGGGTGAGATTGAAATT+GGG + chr2_3:52014099-52014118 None:intergenic 35.0%
! AGTTTTGCGTTCTCAGTTTT+GGG - chr2_3:52014566-52014585 Msa0298110:intron 35.0%
TTACCTTGAGAAAGTGAAGT+AGG + chr2_3:52014640-52014659 None:intergenic 35.0%
! ACAGGTTCCATTCACTATTT+TGG - chr2_3:52014891-52014910 Msa0298110:intron 35.0%
! CAGGTTCCATTCACTATTTT+GGG - chr2_3:52014892-52014911 Msa0298110:intron 35.0%
TCCAAACTTATCCAGCAAAA+TGG + chr2_3:52014920-52014939 None:intergenic 35.0%
TAGGCATTTCCCCTTATTAA+TGG + chr2_3:52014998-52015017 None:intergenic 35.0%
TCAACAAGTGGAACATTTGT+AGG - chr2_3:52015045-52015064 Msa0298110:intron 35.0%
!! TTTGTAGGCTGTTTTATGAC+TGG - chr2_3:52015060-52015079 Msa0298110:intron 35.0%
TGCTTGGGTAATAATCTATG+AGG - chr2_3:52015207-52015226 Msa0298110:intron 35.0%
! TCTATGAGGTATGTGTCATT+TGG - chr2_3:52015221-52015240 Msa0298110:intron 35.0%
TTAACAATTGTGCTCATGGA+AGG - chr2_3:52015291-52015310 Msa0298110:five_prime_UTR 35.0%
!!! GCTTATTTTTTGCTTGGTAG+AGG - chr2_3:52015662-52015681 Msa0298110:five_prime_UTR 35.0%
!!! GTTTTTTGGAATTTTGAGGC+AGG - chr2_3:52015759-52015778 Msa0298110:five_prime_UTR 35.0%
!!! TTATGCTTTTGGTCTTATGC+TGG - chr2_3:52015783-52015802 Msa0298110:five_prime_UTR 35.0%
GATGGAGAAGAAAAATCGAA+TGG - chr2_3:52015894-52015913 Msa0298110:five_prime_UTR 35.0%
AAATTGAGAAAGGTGGAGAG+AGG + chr2_3:52013988-52014007 None:intergenic 40.0%
GGGAATGAAATTGAGAAAGG+TGG + chr2_3:52013995-52014014 None:intergenic 40.0%
CGTGGGAATGAAATTGAGAA+AGG + chr2_3:52013998-52014017 None:intergenic 40.0%
!! GAGATTGAAATGGAGGCATT+AGG + chr2_3:52014038-52014057 None:intergenic 40.0%
AGAGAGTGAGATTGAAATGG+AGG + chr2_3:52014045-52014064 None:intergenic 40.0%
CATGTTGAGGGTTGAAATTG+AGG + chr2_3:52014116-52014135 None:intergenic 40.0%
! GATTGAAATTGGGGGTTTTG+GGG + chr2_3:52014177-52014196 None:intergenic 40.0%
! GAGATTGAAATTGGGGGTTT+TGG + chr2_3:52014179-52014198 None:intergenic 40.0%
TGAGGGTGAGATTGAAATTG+GGG + chr2_3:52014098-52014117 None:intergenic 40.0%
GTTGAGGGTGAGATTGAAAT+TGG + chr2_3:52014188-52014207 None:intergenic 40.0%
CTGTCTCAGATTCATGTTGA+GGG + chr2_3:52014203-52014222 None:intergenic 40.0%
! CAGTTTTGCGTTCTCAGTTT+TGG - chr2_3:52014565-52014584 Msa0298110:intron 40.0%
!! TGGGCAATTTGTTTTCGTCA+AGG - chr2_3:52014585-52014604 Msa0298110:intron 40.0%
!! AAAGAATCGGGAGCCATTTT+GGG - chr2_3:52014833-52014852 Msa0298110:five_prime_UTR 40.0%
ATGGCTCCCAAAATAGTGAA+TGG + chr2_3:52014901-52014920 None:intergenic 40.0%
!! TATTTTGGGAGCCATTTTGC+TGG - chr2_3:52014906-52014925 Msa0298110:intron 40.0%
!! GCCATTTTGCTGGATAAGTT+TGG - chr2_3:52014916-52014935 Msa0298110:intron 40.0%
TGGAAGAAGACCGCTTATAA+CGG - chr2_3:52014936-52014955 Msa0298110:intron 40.0%
GCTGTATTATTCCATGGATC+AGG - chr2_3:52015175-52015194 Msa0298110:intron 40.0%
! ATCAGGATCAGAACTTGCTT+GGG - chr2_3:52015192-52015211 Msa0298110:intron 40.0%
! TCTCTTTTCAGTGTGTGACT+TGG + chr2_3:52015330-52015349 None:intergenic 40.0%
AACCGAATACAAGGTAGATG+CGG - chr2_3:52015435-52015454 Msa0298110:five_prime_UTR 40.0%
ATATCCACAAAGTTGCGGAT+AGG - chr2_3:52015487-52015506 Msa0298110:five_prime_UTR 40.0%
!! ACACTTGCAATTCTGAAGTG+AGG + chr2_3:52015545-52015564 None:intergenic 40.0%
AAAAATAAGCCAGCTGCACA+GGG + chr2_3:52015652-52015671 None:intergenic 40.0%
AAAAAATAAGCCAGCTGCAC+AGG + chr2_3:52015653-52015672 None:intergenic 40.0%
!!! CAGCTGGCTTATTTTTTGCT+TGG - chr2_3:52015656-52015675 Msa0298110:five_prime_UTR 40.0%
!!! TGTGTTGCAGGGTTGTTTTT+TGG - chr2_3:52015745-52015764 Msa0298110:five_prime_UTR 40.0%
! ATACCTCAGCCTCATCATTT+TGG - chr2_3:52015816-52015835 Msa0298110:five_prime_UTR 40.0%
! TACCTCAGCCTCATCATTTT+GGG - chr2_3:52015817-52015836 Msa0298110:five_prime_UTR 40.0%
!! CTCATCATTTTGGGAAGGTT+GGG - chr2_3:52015826-52015845 Msa0298110:five_prime_UTR 40.0%
AAATTGAGAAAGGTGGAGAG+AGG + chr2_3:52013988-52014007 None:intergenic 40.0%
GGGAATGAAATTGAGAAAGG+TGG + chr2_3:52013995-52014014 None:intergenic 40.0%
CGTGGGAATGAAATTGAGAA+AGG + chr2_3:52013998-52014017 None:intergenic 40.0%
!! GAGATTGAAATGGAGGCATT+AGG + chr2_3:52014038-52014057 None:intergenic 40.0%
AGAGAGTGAGATTGAAATGG+AGG + chr2_3:52014045-52014064 None:intergenic 40.0%
CATGTTGAGGGTTGAAATTG+AGG + chr2_3:52014116-52014135 None:intergenic 40.0%
! GATTGAAATTGGGGGTTTTG+GGG + chr2_3:52014177-52014196 None:intergenic 40.0%
! GAGATTGAAATTGGGGGTTT+TGG + chr2_3:52014179-52014198 None:intergenic 40.0%
TGAGGGTGAGATTGAAATTG+GGG + chr2_3:52014098-52014117 None:intergenic 40.0%
GTTGAGGGTGAGATTGAAAT+TGG + chr2_3:52014188-52014207 None:intergenic 40.0%
CTGTCTCAGATTCATGTTGA+GGG + chr2_3:52014203-52014222 None:intergenic 40.0%
! CAGTTTTGCGTTCTCAGTTT+TGG - chr2_3:52014565-52014584 Msa0298110:intron 40.0%
!! TGGGCAATTTGTTTTCGTCA+AGG - chr2_3:52014585-52014604 Msa0298110:intron 40.0%
!! AAAGAATCGGGAGCCATTTT+GGG - chr2_3:52014833-52014852 Msa0298110:five_prime_UTR 40.0%
ATGGCTCCCAAAATAGTGAA+TGG + chr2_3:52014901-52014920 None:intergenic 40.0%
!! TATTTTGGGAGCCATTTTGC+TGG - chr2_3:52014906-52014925 Msa0298110:intron 40.0%
!! GCCATTTTGCTGGATAAGTT+TGG - chr2_3:52014916-52014935 Msa0298110:intron 40.0%
TGGAAGAAGACCGCTTATAA+CGG - chr2_3:52014936-52014955 Msa0298110:intron 40.0%
GCTGTATTATTCCATGGATC+AGG - chr2_3:52015175-52015194 Msa0298110:intron 40.0%
! ATCAGGATCAGAACTTGCTT+GGG - chr2_3:52015192-52015211 Msa0298110:intron 40.0%
! TCTCTTTTCAGTGTGTGACT+TGG + chr2_3:52015330-52015349 None:intergenic 40.0%
AACCGAATACAAGGTAGATG+CGG - chr2_3:52015435-52015454 Msa0298110:five_prime_UTR 40.0%
ATATCCACAAAGTTGCGGAT+AGG - chr2_3:52015487-52015506 Msa0298110:five_prime_UTR 40.0%
!! ACACTTGCAATTCTGAAGTG+AGG + chr2_3:52015545-52015564 None:intergenic 40.0%
AAAAATAAGCCAGCTGCACA+GGG + chr2_3:52015652-52015671 None:intergenic 40.0%
AAAAAATAAGCCAGCTGCAC+AGG + chr2_3:52015653-52015672 None:intergenic 40.0%
!!! CAGCTGGCTTATTTTTTGCT+TGG - chr2_3:52015656-52015675 Msa0298110:five_prime_UTR 40.0%
!!! TGTGTTGCAGGGTTGTTTTT+TGG - chr2_3:52015745-52015764 Msa0298110:five_prime_UTR 40.0%
! ATACCTCAGCCTCATCATTT+TGG - chr2_3:52015816-52015835 Msa0298110:five_prime_UTR 40.0%
! TACCTCAGCCTCATCATTTT+GGG - chr2_3:52015817-52015836 Msa0298110:five_prime_UTR 40.0%
!! CTCATCATTTTGGGAAGGTT+GGG - chr2_3:52015826-52015845 Msa0298110:five_prime_UTR 40.0%
!!! GTTTTGAAGAGTGGAACCGT+GGG + chr2_3:52014015-52014034 None:intergenic 45.0%
!!! GGCATTAGGGTTTTGAAGAG+TGG + chr2_3:52014024-52014043 None:intergenic 45.0%
AATTGGAGGTGAGATTGGGA+GGG + chr2_3:52014083-52014102 None:intergenic 45.0%
AAATTGGAGGTGAGATTGGG+AGG + chr2_3:52014084-52014103 None:intergenic 45.0%
GAGGGTGAGATTGAAATTGG+AGG + chr2_3:52014097-52014116 None:intergenic 45.0%
GAGGGAGAGATTCATGTTGA+GGG + chr2_3:52014128-52014147 None:intergenic 45.0%
TGAGGGAGAGATTCATGTTG+AGG + chr2_3:52014129-52014148 None:intergenic 45.0%
GAGGGTGAGATTGAAATTGG+GGG + chr2_3:52014097-52014116 None:intergenic 45.0%
GCTGTCTCAGATTCATGTTG+AGG + chr2_3:52014204-52014223 None:intergenic 45.0%
GTAACGAAGTAACGTCGTTG+TGG + chr2_3:52014309-52014328 None:intergenic 45.0%
GTTACTTCGTTACTTCGCGA+TGG - chr2_3:52014316-52014335 Msa0298110:intron 45.0%
TTACTTCGCGATGGTGTGTT+TGG - chr2_3:52014325-52014344 Msa0298110:intron 45.0%
GAAGAATCGCGAAGGAATGA+AGG + chr2_3:52014384-52014403 None:intergenic 45.0%
TCCTTCGCGATTCTTCTTCA+AGG - chr2_3:52014388-52014407 Msa0298110:intron 45.0%
ACCTTGAAGAAGAATCGCGA+AGG + chr2_3:52014392-52014411 None:intergenic 45.0%
TCGCCTACTTCACTTTCTCA+AGG - chr2_3:52014634-52014653 Msa0298110:intron 45.0%
! GAAAGAATCGGGAGCCATTT+TGG - chr2_3:52014832-52014851 Msa0298110:five_prime_UTR 45.0%
TGCATGTAGGCTTCAACAAG+TGG - chr2_3:52015033-52015052 Msa0298110:intron 45.0%
! CAAGTTCTGATCCTGATCCA+TGG + chr2_3:52015189-52015208 None:intergenic 45.0%
! GATCAGGATCAGAACTTGCT+TGG - chr2_3:52015191-52015210 Msa0298110:intron 45.0%
CACCGCATCTACCTTGTATT+CGG + chr2_3:52015440-52015459 None:intergenic 45.0%
!! GTAGATGCGGTGTTGATGTA+CGG - chr2_3:52015448-52015467 Msa0298110:five_prime_UTR 45.0%
GAAACCTATCCGCAACTTTG+TGG + chr2_3:52015494-52015513 None:intergenic 45.0%
! TTTTGCTTGGTAGAGGCTGT+CGG - chr2_3:52015669-52015688 Msa0298110:five_prime_UTR 45.0%
!!! AGCGTTTGGTTTTGTGTTGC+AGG - chr2_3:52015733-52015752 Msa0298110:five_prime_UTR 45.0%
!!! GCGTTTGGTTTTGTGTTGCA+GGG - chr2_3:52015734-52015753 Msa0298110:five_prime_UTR 45.0%
! TTGAGGCAGGGTTATGCTTT+TGG - chr2_3:52015772-52015791 Msa0298110:five_prime_UTR 45.0%
GAGGCTGAGGTATACACAAA+TGG + chr2_3:52015809-52015828 None:intergenic 45.0%
TTCCCAAAATGATGAGGCTG+AGG + chr2_3:52015822-52015841 None:intergenic 45.0%
!! TCAGCCTCATCATTTTGGGA+AGG - chr2_3:52015821-52015840 Msa0298110:five_prime_UTR 45.0%
CCAACCTTCCCAAAATGATG+AGG + chr2_3:52015828-52015847 None:intergenic 45.0%
!! CCTCATCATTTTGGGAAGGT+TGG - chr2_3:52015825-52015844 Msa0298110:five_prime_UTR 45.0%
CAGTCACGTTCACAAAGAAG+AGG + chr2_3:52015871-52015890 None:intergenic 45.0%
!!! GTTTTGAAGAGTGGAACCGT+GGG + chr2_3:52014015-52014034 None:intergenic 45.0%
!!! GGCATTAGGGTTTTGAAGAG+TGG + chr2_3:52014024-52014043 None:intergenic 45.0%
AATTGGAGGTGAGATTGGGA+GGG + chr2_3:52014083-52014102 None:intergenic 45.0%
AAATTGGAGGTGAGATTGGG+AGG + chr2_3:52014084-52014103 None:intergenic 45.0%
GAGGGTGAGATTGAAATTGG+AGG + chr2_3:52014097-52014116 None:intergenic 45.0%
GAGGGAGAGATTCATGTTGA+GGG + chr2_3:52014128-52014147 None:intergenic 45.0%
TGAGGGAGAGATTCATGTTG+AGG + chr2_3:52014129-52014148 None:intergenic 45.0%
GAGGGTGAGATTGAAATTGG+GGG + chr2_3:52014097-52014116 None:intergenic 45.0%
GCTGTCTCAGATTCATGTTG+AGG + chr2_3:52014204-52014223 None:intergenic 45.0%
GTAACGAAGTAACGTCGTTG+TGG + chr2_3:52014309-52014328 None:intergenic 45.0%
GTTACTTCGTTACTTCGCGA+TGG - chr2_3:52014316-52014335 Msa0298110:intron 45.0%
TTACTTCGCGATGGTGTGTT+TGG - chr2_3:52014325-52014344 Msa0298110:intron 45.0%
GAAGAATCGCGAAGGAATGA+AGG + chr2_3:52014384-52014403 None:intergenic 45.0%
TCCTTCGCGATTCTTCTTCA+AGG - chr2_3:52014388-52014407 Msa0298110:intron 45.0%
ACCTTGAAGAAGAATCGCGA+AGG + chr2_3:52014392-52014411 None:intergenic 45.0%
TCGCCTACTTCACTTTCTCA+AGG - chr2_3:52014634-52014653 Msa0298110:intron 45.0%
! GAAAGAATCGGGAGCCATTT+TGG - chr2_3:52014832-52014851 Msa0298110:five_prime_UTR 45.0%
TGCATGTAGGCTTCAACAAG+TGG - chr2_3:52015033-52015052 Msa0298110:intron 45.0%
! CAAGTTCTGATCCTGATCCA+TGG + chr2_3:52015189-52015208 None:intergenic 45.0%
! GATCAGGATCAGAACTTGCT+TGG - chr2_3:52015191-52015210 Msa0298110:intron 45.0%
CACCGCATCTACCTTGTATT+CGG + chr2_3:52015440-52015459 None:intergenic 45.0%
!! GTAGATGCGGTGTTGATGTA+CGG - chr2_3:52015448-52015467 Msa0298110:five_prime_UTR 45.0%
GAAACCTATCCGCAACTTTG+TGG + chr2_3:52015494-52015513 None:intergenic 45.0%
! TTTTGCTTGGTAGAGGCTGT+CGG - chr2_3:52015669-52015688 Msa0298110:five_prime_UTR 45.0%
!!! AGCGTTTGGTTTTGTGTTGC+AGG - chr2_3:52015733-52015752 Msa0298110:five_prime_UTR 45.0%
!!! GCGTTTGGTTTTGTGTTGCA+GGG - chr2_3:52015734-52015753 Msa0298110:five_prime_UTR 45.0%
! TTGAGGCAGGGTTATGCTTT+TGG - chr2_3:52015772-52015791 Msa0298110:five_prime_UTR 45.0%
GAGGCTGAGGTATACACAAA+TGG + chr2_3:52015809-52015828 None:intergenic 45.0%
TTCCCAAAATGATGAGGCTG+AGG + chr2_3:52015822-52015841 None:intergenic 45.0%
!! TCAGCCTCATCATTTTGGGA+AGG - chr2_3:52015821-52015840 Msa0298110:five_prime_UTR 45.0%
CCAACCTTCCCAAAATGATG+AGG + chr2_3:52015828-52015847 None:intergenic 45.0%
!! CCTCATCATTTTGGGAAGGT+TGG - chr2_3:52015825-52015844 Msa0298110:five_prime_UTR 45.0%
CAGTCACGTTCACAAAGAAG+AGG + chr2_3:52015871-52015890 None:intergenic 45.0%
!!! GGTTTTGAAGAGTGGAACCG+TGG + chr2_3:52014016-52014035 None:intergenic 50.0%
GAAGTGAGGTGAAGAAGTCG+CGG + chr2_3:52015531-52015550 None:intergenic 50.0%
AAGGTTGGGCTGCTTGTGTT+TGG - chr2_3:52015840-52015859 Msa0298110:five_prime_UTR 50.0%
TTGTGAACGTGACTGCTCGA+TGG - chr2_3:52015876-52015895 Msa0298110:five_prime_UTR 50.0%
!!! GGTTTTGAAGAGTGGAACCG+TGG + chr2_3:52014016-52014035 None:intergenic 50.0%
GAAGTGAGGTGAAGAAGTCG+CGG + chr2_3:52015531-52015550 None:intergenic 50.0%
AAGGTTGGGCTGCTTGTGTT+TGG - chr2_3:52015840-52015859 Msa0298110:five_prime_UTR 50.0%
TTGTGAACGTGACTGCTCGA+TGG - chr2_3:52015876-52015895 Msa0298110:five_prime_UTR 50.0%
!! GGTGAGATTGGGAGGGTGTT+AGG + chr2_3:52014076-52014095 None:intergenic 55.0%
ACCACGTGCAGAAGCAGGTT+GGG + chr2_3:52014360-52014379 None:intergenic 55.0%
AACCACGTGCAGAAGCAGGT+TGG + chr2_3:52014361-52014380 None:intergenic 55.0%
AAGGAACCACGTGCAGAAGC+AGG + chr2_3:52014365-52014384 None:intergenic 55.0%
! TTCGTCAAGGCATTTCGCCG+TGG - chr2_3:52014598-52014617 Msa0298110:intron 55.0%
!!! GGCAGTACTGCTGCTGGTTT+TGG - chr2_3:52015690-52015709 Msa0298110:five_prime_UTR 55.0%
TTGCTGCTGACAGCAGCGTT+TGG - chr2_3:52015719-52015738 Msa0298110:five_prime_UTR 55.0%
!! GGTGAGATTGGGAGGGTGTT+AGG + chr2_3:52014076-52014095 None:intergenic 55.0%
ACCACGTGCAGAAGCAGGTT+GGG + chr2_3:52014360-52014379 None:intergenic 55.0%
AACCACGTGCAGAAGCAGGT+TGG + chr2_3:52014361-52014380 None:intergenic 55.0%
AAGGAACCACGTGCAGAAGC+AGG + chr2_3:52014365-52014384 None:intergenic 55.0%
! TTCGTCAAGGCATTTCGCCG+TGG - chr2_3:52014598-52014617 Msa0298110:intron 55.0%
!!! GGCAGTACTGCTGCTGGTTT+TGG - chr2_3:52015690-52015709 Msa0298110:five_prime_UTR 55.0%
TTGCTGCTGACAGCAGCGTT+TGG - chr2_3:52015719-52015738 Msa0298110:five_prime_UTR 55.0%
CGTCGTTGTGGCTGACAGAG+AGG + chr2_3:52014297-52014316 None:intergenic 60.0%
ACCCAACCTGCTTCTGCACG+TGG - chr2_3:52014356-52014375 Msa0298110:intron 60.0%
GCGAGCTGAAGTGCGAACCA+CGG + chr2_3:52014618-52014637 None:intergenic 60.0%
CGTCGTTGTGGCTGACAGAG+AGG + chr2_3:52014297-52014316 None:intergenic 60.0%
ACCCAACCTGCTTCTGCACG+TGG - chr2_3:52014356-52014375 Msa0298110:intron 60.0%
GCGAGCTGAAGTGCGAACCA+CGG + chr2_3:52014618-52014637 None:intergenic 60.0%
! CGTTGGTGTCCCTGTGCAGC+TGG - chr2_3:52015640-52015659 Msa0298110:five_prime_UTR 65.0%
GCTGTCGGCAGTACTGCTGC+TGG - chr2_3:52015684-52015703 Msa0298110:five_prime_UTR 65.0%
! CGTTGGTGTCCCTGTGCAGC+TGG - chr2_3:52015640-52015659 Msa0298110:five_prime_UTR 65.0%
GCTGTCGGCAGTACTGCTGC+TGG - chr2_3:52015684-52015703 Msa0298110:five_prime_UTR 65.0%
Chromosome Type Strat End Strand Name
chr2_3 gene 52013978 52015924 52013978 ID=Msa0298110;Name=Msa0298110
chr2_3 mRNA 52013978 52015924 52013978 ID=Msa0298110-mRNA-1;Parent=Msa0298110;Name=Msa0298110-mRNA-1;_AED=0.36;_eAED=0.36;_QI=828|0.75|0.8|1|0.25|0.4|5|0|81
chr2_3 exon 52013978 52014218 52013978 ID=Msa0298110-mRNA-1:exon:16614;Parent=Msa0298110-mRNA-1
chr2_3 exon 52014674 52014705 52014674 ID=Msa0298110-mRNA-1:exon:16613;Parent=Msa0298110-mRNA-1
chr2_3 exon 52014801 52014860 52014801 ID=Msa0298110-mRNA-1:exon:16612;Parent=Msa0298110-mRNA-1
chr2_3 exon 52014945 52015007 52014945 ID=Msa0298110-mRNA-1:exon:16611;Parent=Msa0298110-mRNA-1
chr2_3 exon 52015247 52015924 52015247 ID=Msa0298110-mRNA-1:exon:16610;Parent=Msa0298110-mRNA-1
chr2_3 five_prime_UTR 52015247 52015924 52015247 ID=Msa0298110-mRNA-1:five_prime_utr;Parent=Msa0298110-mRNA-1
chr2_3 five_prime_UTR 52014945 52015007 52014945 ID=Msa0298110-mRNA-1:five_prime_utr;Parent=Msa0298110-mRNA-1
chr2_3 five_prime_UTR 52014801 52014860 52014801 ID=Msa0298110-mRNA-1:five_prime_utr;Parent=Msa0298110-mRNA-1
chr2_3 five_prime_UTR 52014679 52014705 52014679 ID=Msa0298110-mRNA-1:five_prime_utr;Parent=Msa0298110-mRNA-1
chr2_3 CDS 52014674 52014678 52014674 ID=Msa0298110-mRNA-1:cds;Parent=Msa0298110-mRNA-1
chr2_3 CDS 52013978 52014218 52013978 ID=Msa0298110-mRNA-1:cds;Parent=Msa0298110-mRNA-1
Gene Sequence

>Msa0298110

ATGAGGCTGTCGGCAGTACTGCTGCTGGTTTTGGTGTTGTTTGCTGCTGACAGCAGCGTTTGGTTTTGTGTTGCAGGGTTGTTTTTTGGAATTTTGAGGCAGGGTTATGCTTTTGGTCTTATGCTGGCCATTTGTGTATACCTCAGCCTCATCATTTTGGGAAGGTTGGGCTGCTTGTGTTTGGTTGTTCCTCTTCTTTGTGAACGTGACTGCTCGATGGAGAAGAAAAATCGAATGGATTTTTAG
Protein sequence

>Msa0298110

MRLSAVLLLVLVLFAADSSVWFCVAGLFFGILRQGYAFGLMLAICVYLSLIILGRLGCLCLVVPLLCERDCSMEKKNRMDF*