AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1034890

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1034890 MtrunA17_Chr7g0258091 100.000 145 0 0 1 145 1 145 2.00e-100 284
Msa1034890 MtrunA17_Chr6g0472581 83.448 145 23 1 1 145 1 144 4.51e-73 215
Msa1034890 MtrunA17_Chr6g0471881 95.349 43 2 0 103 145 1 43 2.46e-24 88.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1034890 AT5G51940.1 79.452 146 27 2 1 145 1 144 2.05e-70 208
Msa1034890 AT2G04630.1 78.082 146 29 2 1 145 1 144 6.78e-69 204

Find 89 sgRNAs with CRISPR-Local

Find 266 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTATAGCATCCTATTAGTTT+TGG 0.218248 7_1:+70604325 Msa1034890:three_prime_UTR
CATATACTTGGAAGTCCTTC+TGG 0.228416 7_1:-70601431 None:intergenic
TTGAAGTGATCCTTCCTAAA+TGG 0.237005 7_1:+70604498 Msa1034890:three_prime_UTR
CCGCTCTCGTAGCTCCTTCA+TGG 0.261897 7_1:-70603964 None:intergenic
AAGTATGGTACCTCAATTTC+AGG 0.283991 7_1:-70601245 None:intergenic
AGGTCATCATTCTTGTTATC+AGG 0.290460 7_1:-70601346 None:intergenic
ACCTCTCATAGTATTTATCT+TGG 0.327441 7_1:+70604257 Msa1034890:three_prime_UTR
AATACCATTCTTAAGAAATA+AGG 0.327574 7_1:+70604560 Msa1034890:three_prime_UTR
AATACACACCCAAAACTAAT+AGG 0.329429 7_1:-70604334 None:intergenic
ACCACCCACTTGTCTCTTCC+AGG 0.359364 7_1:-70604186 None:intergenic
AAGTAGCGACGGATGGTAAA+AGG 0.371530 7_1:-70603993 None:intergenic
GTCCTTCTGGGACGTTCAAC+CGG 0.388301 7_1:-70601418 None:intergenic
CAACTGTCAGGATGCACTAC+TGG 0.392520 7_1:+70604466 Msa1034890:three_prime_UTR
GTACCCGCGCTCTTCAAATC+AGG 0.398664 7_1:+70601482 Msa1034890:CDS
ATGTCCTTATTTCTTAAGAA+TGG 0.400994 7_1:-70604564 None:intergenic
GTCAAACGAAATCACATGTA+AGG 0.406142 7_1:+70604650 Msa1034890:three_prime_UTR
CTATGCTCCTACCATAAAAC+TGG 0.406310 7_1:+70604301 Msa1034890:three_prime_UTR
TTTATCTTGGATTTGTATGA+AGG 0.410241 7_1:+70604270 Msa1034890:three_prime_UTR
CGGATTAAAGCAAAAGAAAC+AGG 0.414481 7_1:-70604366 None:intergenic
GTGATTTCGTTTGACTGAAA+AGG 0.415928 7_1:-70604642 None:intergenic
AACTCTTCAGATTGCCATGA+AGG 0.418174 7_1:+70603950 Msa1034890:intron
ATTGTCCAGCTATGAAGATT+GGG 0.418900 7_1:+70604136 Msa1034890:intron
ACCAATTGAAACTGAGGAAA+AGG 0.424157 7_1:+70601384 Msa1034890:CDS
TATGAGCGTGCTAGGATTCT+CGG 0.433316 7_1:+70601460 Msa1034890:CDS
AATTGTCCAGCTATGAAGAT+TGG 0.435283 7_1:+70604135 Msa1034890:intron
CATGACAAATTTGACTTTGG+AGG 0.440026 7_1:+70604610 Msa1034890:three_prime_UTR
CTCATACTTAGTCATATACT+TGG 0.444475 7_1:-70601443 None:intergenic
ACCTGTCATGGTTGAGTTGG+AGG 0.445018 7_1:+70602886 Msa1034890:CDS
AGCTGATTGTGGAAGACTCC+TGG 0.445700 7_1:+70604168 Msa1034890:CDS
GGACATGACAAATTTGACTT+TGG 0.450391 7_1:+70604607 Msa1034890:three_prime_UTR
GGTACCTCAATTTCAGGCTC+TGG 0.453336 7_1:-70601239 None:intergenic
CGGCCATGGTTGTTGAATTG+AGG 0.464945 7_1:-70601084 None:intergenic
AGTAGTATTTACCTTCCATC+AGG 0.479574 7_1:-70604017 None:intergenic
GCCACCAGAGCCTGAAATTG+AGG 0.481155 7_1:+70601235 Msa1034890:CDS
ATATACTTGGAAGTCCTTCT+GGG 0.481308 7_1:-70601430 None:intergenic
AGTGCATCCTGACAGTTGAT+TGG 0.481754 7_1:-70604461 None:intergenic
CCCTCCAACTCAACCATGAC+AGG 0.481823 7_1:-70602887 None:intergenic
TTCGTAGTCATCGTCGGCCA+TGG 0.482815 7_1:-70601098 None:intergenic
TTGGTTCTCCTTCTGCATCT+AGG 0.484900 7_1:-70601366 None:intergenic
GGCATTCATTCCGTCCGAGT+GGG 0.488022 7_1:+70601032 Msa1034890:five_prime_UTR
AATGGTTAAATTACCTCAAG+TGG 0.493654 7_1:-70602920 None:intergenic
TGTTTGTCCAATCAACTGTC+AGG 0.500154 7_1:+70604454 Msa1034890:three_prime_UTR
CCTGTCATGGTTGAGTTGGA+GGG 0.500999 7_1:+70602887 Msa1034890:CDS
ATGACAAATTTGACTTTGGA+GGG 0.505240 7_1:+70604611 Msa1034890:three_prime_UTR
GCAGGAAGGTGCAGAGGAGG+AGG 0.505888 7_1:+70601321 Msa1034890:intron
ACCTCAATTTCAGGCTCTGG+TGG 0.507724 7_1:-70601236 None:intergenic
GGGAGTTGATGAGCTGATTG+TGG 0.508386 7_1:+70604157 Msa1034890:CDS
GGAAAAGGAAGATGCTGCTC+CGG 0.509327 7_1:+70601399 Msa1034890:CDS
CATTCATTCCGTCCGAGTGG+GGG 0.516711 7_1:+70601034 Msa1034890:five_prime_UTR
TGACTAAGTATGAGCGTGCT+AGG 0.520533 7_1:+70601452 Msa1034890:CDS
TCTGTGTATGATAAATCTCT+AGG 0.530461 7_1:+70604411 Msa1034890:three_prime_UTR
GATTGCTTGAACTGCTAGTG+TGG 0.538461 7_1:+70604213 Msa1034890:exon
ATCCGTCGCTACTTGCCTGA+TGG 0.540830 7_1:+70604002 Msa1034890:CDS
TGCACCTGTCATGGTTGAGT+TGG 0.547769 7_1:+70602883 Msa1034890:CDS
ACAGTTGATTGGACAAACAT+AGG 0.552663 7_1:-70604450 None:intergenic
ATTATGAAGTCGAAAATCAC+TGG 0.553966 7_1:-70604534 None:intergenic
GCATTCATTCCGTCCGAGTG+GGG 0.554792 7_1:+70601033 Msa1034890:five_prime_UTR
AGTCAAATTTGTCATGTCCT+CGG 0.558920 7_1:-70604603 None:intergenic
TTCCATCAGGCAAGTAGCGA+CGG 0.559984 7_1:-70604004 None:intergenic
TGTGCAGGAAGGTGCAGAGG+AGG 0.563259 7_1:+70601318 Msa1034890:intron
ATTGCTTGAACTGCTAGTGT+GGG 0.565907 7_1:+70604214 Msa1034890:exon
TCCAAGATAAATACTATGAG+AGG 0.574798 7_1:-70604258 None:intergenic
ATTCATTCCGTCCGAGTGGG+GGG 0.580299 7_1:+70601035 Msa1034890:five_prime_UTR
GTCGCTACTTGCCTGATGGA+AGG 0.581718 7_1:+70604006 Msa1034890:CDS
AAACCTGATTTGAAGAGCGC+GGG 0.585792 7_1:-70601485 None:intergenic
GACTCCTGGAAGAGACAAGT+GGG 0.586530 7_1:+70604182 Msa1034890:CDS
AGGAGAACCAATTGAAACTG+AGG 0.593065 7_1:+70601378 Msa1034890:CDS
CAGTATGAATGCACCTGTCA+TGG 0.596445 7_1:+70602874 Msa1034890:intron
TCAAACGAAATCACATGTAA+GGG 0.600071 7_1:+70604651 Msa1034890:three_prime_UTR
ATCAGGCAAGTAGCGACGGA+TGG 0.606515 7_1:-70604000 None:intergenic
CTTCCTCAATTCAACAACCA+TGG 0.610028 7_1:+70601081 Msa1034890:exon
CTGTCATGGTTGAGTTGGAG+GGG 0.614709 7_1:+70602888 Msa1034890:CDS
AATGATGACCTAGATGCAGA+AGG 0.620982 7_1:+70601358 Msa1034890:CDS
TGTCATGGTTGAGTTGGAGG+GGG 0.622677 7_1:+70602889 Msa1034890:CDS
TAAACCTGATTTGAAGAGCG+CGG 0.623407 7_1:-70601486 None:intergenic
GACTACGAAGATATCGACAT+GGG 0.632471 7_1:+70601112 Msa1034890:CDS
ATGGTTAAATTACCTCAAGT+GGG 0.636541 7_1:-70602919 None:intergenic
AGACTCCTGGAAGAGACAAG+TGG 0.644950 7_1:+70604181 Msa1034890:CDS
TTGTCCAGCTATGAAGATTG+GGG 0.646097 7_1:+70604137 Msa1034890:intron
TTGCTTGAACTGCTAGTGTG+GGG 0.651050 7_1:+70604215 Msa1034890:three_prime_UTR
AGGCATTCATTCCGTCCGAG+TGG 0.656041 7_1:+70601031 Msa1034890:five_prime_UTR
GATATCTTCGTAGTCATCGT+CGG 0.659789 7_1:-70601104 None:intergenic
TGACTACGAAGATATCGACA+TGG 0.677574 7_1:+70601111 Msa1034890:CDS
GCATGTGCAGGAAGGTGCAG+AGG 0.686778 7_1:+70601315 Msa1034890:intron
ATTAAAACGATGCAGGTACG+AGG 0.688503 7_1:+70601208 Msa1034890:intron
CCATGAAGGAGCTACGAGAG+CGG 0.693186 7_1:+70603964 Msa1034890:CDS
AAAACGATGCAGGTACGAGG+AGG 0.694919 7_1:+70601211 Msa1034890:intron
TCCTGGAAGAGACAAGTGGG+TGG 0.726072 7_1:+70604185 Msa1034890:CDS
ACTACGAAGATATCGACATG+GGG 0.760905 7_1:+70601113 Msa1034890:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CACATAATTAATAATTAATT+AGG + chr7_1:70601162-70601181 Msa1034890:intron 10.0%
!!! TCTTCTTTTTTTCTTTAATT+AGG + chr7_1:70601267-70601286 Msa1034890:intron 15.0%
!!! CTTCTTTTTTTCTTTAATTA+GGG + chr7_1:70601268-70601287 Msa1034890:intron 15.0%
!!! ATGATTGTTTAAGATTTTAA+AGG - chr7_1:70602806-70602825 None:intergenic 15.0%
!!! AATAGGCTTATTTTTATATT+AGG + chr7_1:70603324-70603343 Msa1034890:intron 15.0%
!! AACAAAAAACAATATAAGGA+AGG + chr7_1:70601011-70601030 Msa1034890:five_prime_UTR 20.0%
!! AAAGAAAAAAAGAAGAAGTA+TGG - chr7_1:70601263-70601282 None:intergenic 20.0%
!! AATAAGCAAAATCATACATA+GGG - chr7_1:70601528-70601547 None:intergenic 20.0%
!! TAAATTGAACTATGTTAACA+TGG + chr7_1:70601750-70601769 Msa1034890:intron 20.0%
!! AAATTGAACTATGTTAACAT+GGG + chr7_1:70601751-70601770 Msa1034890:intron 20.0%
!! TTAACATATACTTGTGTAAT+AGG + chr7_1:70601781-70601800 Msa1034890:intron 20.0%
!! ATTTATGTAACATGCAATAA+AGG - chr7_1:70602377-70602396 None:intergenic 20.0%
!! TTTCTATATTTCAATTGAGT+GGG - chr7_1:70603055-70603074 None:intergenic 20.0%
!!! TTTTCTATATTTCAATTGAG+TGG - chr7_1:70603056-70603075 None:intergenic 20.0%
!! TTGAAAATTATAGCATAAAC+TGG + chr7_1:70603469-70603488 Msa1034890:intron 20.0%
!! TGAAAATTATAGCATAAACT+GGG + chr7_1:70603470-70603489 Msa1034890:intron 20.0%
!! TTTAAAAAAAGTTTCATCGA+TGG - chr7_1:70603536-70603555 None:intergenic 20.0%
!! TTAAATCCTAAATTAACATG+TGG - chr7_1:70603638-70603657 None:intergenic 20.0%
!! TTAGGATTTAAATATTCTCA+TGG + chr7_1:70603647-70603666 Msa1034890:intron 20.0%
!! TACTGTTTGTTTATTAAGTT+GGG + chr7_1:70604036-70604055 Msa1034890:intron 20.0%
!! AATACCATTCTTAAGAAATA+AGG + chr7_1:70604560-70604579 Msa1034890:three_prime_UTR 20.0%
! GAATAAGCAAAATCATACAT+AGG - chr7_1:70601529-70601548 None:intergenic 25.0%
! ATTAGTATTTGTGTTAGCTA+TGG + chr7_1:70601549-70601568 Msa1034890:intron 25.0%
!!! ATCAATGGGTTATGTAAAAT+GGG + chr7_1:70601579-70601598 Msa1034890:intron 25.0%
!! TGTATGTTCTAAATAGAGTA+AGG - chr7_1:70601723-70601742 None:intergenic 25.0%
! TGATAATTAAGTTCTTGTTG+AGG + chr7_1:70601971-70601990 Msa1034890:intron 25.0%
!! AACTTTTATGTTGGAGAATA+TGG - chr7_1:70601997-70602016 None:intergenic 25.0%
! AAGTTGTCAAACAAAATTTG+AGG + chr7_1:70602078-70602097 Msa1034890:intron 25.0%
!! ACATAAATTGACAAGCTTTT+TGG + chr7_1:70602389-70602408 Msa1034890:intron 25.0%
! ACTCTATTGATAAGTTGTTT+GGG - chr7_1:70602460-70602479 None:intergenic 25.0%
! TACTCTATTGATAAGTTGTT+TGG - chr7_1:70602461-70602480 None:intergenic 25.0%
! AAACAACTTATCAATAGAGT+AGG + chr7_1:70602460-70602479 Msa1034890:intron 25.0%
!! TTGCAACTTTTATTGAAGTA+TGG - chr7_1:70602512-70602531 None:intergenic 25.0%
! CTAACAAAAAGTTCTTTCAA+CGG - chr7_1:70602783-70602802 None:intergenic 25.0%
! TTCTATATTTCAATTGAGTG+GGG - chr7_1:70603054-70603073 None:intergenic 25.0%
! AGATCAATAAAGTAGAAGTA+AGG - chr7_1:70603097-70603116 None:intergenic 25.0%
! ACATACATGTATAGTAACAA+GGG + chr7_1:70603234-70603253 Msa1034890:intron 25.0%
!!! AAAAATAAGCCTATTTCTGA+TGG - chr7_1:70603319-70603338 None:intergenic 25.0%
!! TGCTATAATTTTCAAAGTTC+AGG - chr7_1:70603464-70603483 None:intergenic 25.0%
!!! GTTTTATTTTTAGAGTCCTT+TGG + chr7_1:70603686-70603705 Msa1034890:intron 25.0%
!!! TCCATATTTTTTTGTTGTGT+TGG + chr7_1:70603849-70603868 Msa1034890:intron 25.0%
! CTACTGTTTGTTTATTAAGT+TGG + chr7_1:70604035-70604054 Msa1034890:intron 25.0%
! TTTATCTTGGATTTGTATGA+AGG + chr7_1:70604270-70604289 Msa1034890:three_prime_UTR 25.0%
!!! TTATAGCATCCTATTAGTTT+TGG + chr7_1:70604325-70604344 Msa1034890:three_prime_UTR 25.0%
!!! TATAGCATCCTATTAGTTTT+GGG + chr7_1:70604326-70604345 Msa1034890:three_prime_UTR 25.0%
!! ATGTCCTTATTTCTTAAGAA+TGG - chr7_1:70604567-70604586 None:intergenic 25.0%
CTCATACTTAGTCATATACT+TGG - chr7_1:70601446-70601465 None:intergenic 30.0%
!! GATCAATGGGTTATGTAAAA+TGG + chr7_1:70601578-70601597 Msa1034890:intron 30.0%
ATCAATGGATGATGTAAAAC+AGG - chr7_1:70601632-70601651 None:intergenic 30.0%
TTTACATCATCCATTGATCA+AGG + chr7_1:70601634-70601653 Msa1034890:intron 30.0%
TAAGAAATCTACATACGTGT+CGG + chr7_1:70601933-70601952 Msa1034890:intron 30.0%
! AGCTTTTTGGACAACAAAAT+AGG + chr7_1:70602402-70602421 Msa1034890:intron 30.0%
ATTGCAATATGAACCTTACA+AGG - chr7_1:70602435-70602454 None:intergenic 30.0%
CTCTATTGATAAGTTGTTTG+GGG - chr7_1:70602459-70602478 None:intergenic 30.0%
! TTTTATTGAAGTATGGTGCT+TGG - chr7_1:70602505-70602524 None:intergenic 30.0%
ATCTTAAACAATCATACTGC+AGG + chr7_1:70602811-70602830 Msa1034890:intron 30.0%
ACAAGAAAGACAACAAGTTA+TGG - chr7_1:70602846-70602865 None:intergenic 30.0%
ATGGTTAAATTACCTCAAGT+GGG - chr7_1:70602922-70602941 None:intergenic 30.0%
AATGGTTAAATTACCTCAAG+TGG - chr7_1:70602923-70602942 None:intergenic 30.0%
ATACAAACAAGATCAACATC+TGG - chr7_1:70603164-70603183 None:intergenic 30.0%
! TATGCTTAGATCATTGACTA+TGG + chr7_1:70603181-70603200 Msa1034890:intron 30.0%
ACATGTATGTCAGCATATAT+TGG - chr7_1:70603224-70603243 None:intergenic 30.0%
GACATACATGTATAGTAACA+AGG + chr7_1:70603233-70603252 Msa1034890:intron 30.0%
TGATCTCGTCTTCAAAATAT+AGG - chr7_1:70603292-70603311 None:intergenic 30.0%
GATCAAATTCCATCAGAAAT+AGG + chr7_1:70603307-70603326 Msa1034890:intron 30.0%
CTGAACAAATGAACTATAAC+AGG - chr7_1:70603361-70603380 None:intergenic 30.0%
! CTTTCTCCCTTTAAAGAAAA+AGG + chr7_1:70603437-70603456 Msa1034890:intron 30.0%
! TCAGGACCTTTTTCTTTAAA+GGG - chr7_1:70603446-70603465 None:intergenic 30.0%
! TTCAGGACCTTTTTCTTTAA+AGG - chr7_1:70603447-70603466 None:intergenic 30.0%
ATCTGACCACATGTTAATTT+AGG + chr7_1:70603629-70603648 Msa1034890:intron 30.0%
ATAACATGTAGCAATACCAA+AGG - chr7_1:70603705-70603724 None:intergenic 30.0%
GCCAACACAACAAAAAAATA+TGG - chr7_1:70603853-70603872 None:intergenic 30.0%
ACTGATACAAAATGAAACCA+TGG - chr7_1:70603880-70603899 None:intergenic 30.0%
! TTATGTCGTACTTACCATTT+TGG + chr7_1:70604111-70604130 Msa1034890:intron 30.0%
ACCTCTCATAGTATTTATCT+TGG + chr7_1:70604257-70604276 Msa1034890:three_prime_UTR 30.0%
TCCAAGATAAATACTATGAG+AGG - chr7_1:70604261-70604280 None:intergenic 30.0%
AATACACACCCAAAACTAAT+AGG - chr7_1:70604337-70604356 None:intergenic 30.0%
!! CTGTTTCTTTTGCTTTAATC+CGG + chr7_1:70604367-70604386 Msa1034890:three_prime_UTR 30.0%
!!! ATCCGGAATCTTTTTTGTTT+CGG + chr7_1:70604384-70604403 Msa1034890:three_prime_UTR 30.0%
TCTGTGTATGATAAATCTCT+AGG + chr7_1:70604411-70604430 Msa1034890:three_prime_UTR 30.0%
GTTCAAAAATCCATTTAGGA+AGG - chr7_1:70604511-70604530 None:intergenic 30.0%
GCAAGTTCAAAAATCCATTT+AGG - chr7_1:70604515-70604534 None:intergenic 30.0%
ATTATGAAGTCGAAAATCAC+TGG - chr7_1:70604537-70604556 None:intergenic 30.0%
! AGTGATTTTCGACTTCATAA+TGG + chr7_1:70604536-70604555 Msa1034890:three_prime_UTR 30.0%
! ATGACAAATTTGACTTTGGA+GGG + chr7_1:70604611-70604630 Msa1034890:three_prime_UTR 30.0%
TCAAACGAAATCACATGTAA+GGG + chr7_1:70604651-70604670 Msa1034890:three_prime_UTR 30.0%
ACTCATGATTAAAACGATGC+AGG + chr7_1:70601201-70601220 Msa1034890:intron 35.0%
AAGTATGGTACCTCAATTTC+AGG - chr7_1:70601248-70601267 None:intergenic 35.0%
! AGGTCATCATTCTTGTTATC+AGG - chr7_1:70601349-70601368 None:intergenic 35.0%
ACCAATTGAAACTGAGGAAA+AGG + chr7_1:70601384-70601403 Msa1034890:CDS 35.0%
! TCCTTTTCCTCAGTTTCAAT+TGG - chr7_1:70601388-70601407 None:intergenic 35.0%
ATATACTTGGAAGTCCTTCT+GGG - chr7_1:70601433-70601452 None:intergenic 35.0%
AGCTATGGAATCTTGATCAA+TGG + chr7_1:70601564-70601583 Msa1034890:intron 35.0%
GCTATGGAATCTTGATCAAT+GGG + chr7_1:70601565-70601584 Msa1034890:intron 35.0%
TGATGTAAAACAGGACAAAC+TGG - chr7_1:70601623-70601642 None:intergenic 35.0%
!! CTTTTGGTTACCTTGATCAA+TGG - chr7_1:70601647-70601666 None:intergenic 35.0%
CATTGATCAAGGTAACCAAA+AGG + chr7_1:70601645-70601664 Msa1034890:intron 35.0%
ATAGAGTAAGGATGAGAAAG+AGG - chr7_1:70601711-70601730 None:intergenic 35.0%
! GGAGTTGACAACTTTTATGT+TGG - chr7_1:70602006-70602025 None:intergenic 35.0%
!! AAAATTTGAGGAGTGCTGAA+AGG + chr7_1:70602090-70602109 Msa1034890:intron 35.0%
CAAAAATCCAGAATAGATGC+TGG + chr7_1:70602253-70602272 Msa1034890:intron 35.0%
AGCTGTAAAAACGAATACTC+AGG - chr7_1:70602344-70602363 None:intergenic 35.0%
TTTCAAGAAGCTTGACAGTT+AGG - chr7_1:70602554-70602573 None:intergenic 35.0%
GCATAGTAGTGTATTGTACT+GGG - chr7_1:70602620-70602639 None:intergenic 35.0%
TGCATAGTAGTGTATTGTAC+TGG - chr7_1:70602621-70602640 None:intergenic 35.0%
AGGGGAGAATTTAAACCAAA+CGG - chr7_1:70602696-70602715 None:intergenic 35.0%
! CTTTTGTCAAAAAGTTGCCT+TGG - chr7_1:70602747-70602766 None:intergenic 35.0%
TGAATCGCAAAGAGATCAAA+TGG - chr7_1:70602941-70602960 None:intergenic 35.0%
CAGTACTGTAACAAGAAAGA+AGG - chr7_1:70602967-70602986 None:intergenic 35.0%
CATGGTACACTACTTAATGA+AGG - chr7_1:70603120-70603139 None:intergenic 35.0%
ATGTGCAATTGTGCATTACA+TGG - chr7_1:70603138-70603157 None:intergenic 35.0%
CATTTGTTCAGCTTGTAGAT+TGG + chr7_1:70603370-70603389 Msa1034890:intron 35.0%
AAAGTACAAGCTATGGACAA+TGG - chr7_1:70603404-70603423 None:intergenic 35.0%
AGTAGTATTTACCTTCCATC+AGG - chr7_1:70604020-70604039 None:intergenic 35.0%
! AGTTGGGTACTTCTTTTTCT+TGG + chr7_1:70604052-70604071 Msa1034890:intron 35.0%
! GGTACTTCTTTTTCTTGGAT+GGG + chr7_1:70604057-70604076 Msa1034890:intron 35.0%
AAGTACGACATAAAAGATGG+AGG - chr7_1:70604104-70604123 None:intergenic 35.0%
GGTAAGTACGACATAAAAGA+TGG - chr7_1:70604107-70604126 None:intergenic 35.0%
AATTGTCCAGCTATGAAGAT+TGG + chr7_1:70604135-70604154 Msa1034890:intron 35.0%
ATTGTCCAGCTATGAAGATT+GGG + chr7_1:70604136-70604155 Msa1034890:intron 35.0%
!! CTATAAGCCAGTTTTATGGT+AGG - chr7_1:70604311-70604330 None:intergenic 35.0%
! GATGCTATAAGCCAGTTTTA+TGG - chr7_1:70604315-70604334 None:intergenic 35.0%
CGGATTAAAGCAAAAGAAAC+AGG - chr7_1:70604369-70604388 None:intergenic 35.0%
AGCCGAAACAAAAAAGATTC+CGG - chr7_1:70604389-70604408 None:intergenic 35.0%
ACAGTTGATTGGACAAACAT+AGG - chr7_1:70604453-70604472 None:intergenic 35.0%
TTGAAGTGATCCTTCCTAAA+TGG + chr7_1:70604498-70604517 Msa1034890:three_prime_UTR 35.0%
AGTCAAATTTGTCATGTCCT+CGG - chr7_1:70604606-70604625 None:intergenic 35.0%
! GGACATGACAAATTTGACTT+TGG + chr7_1:70604607-70604626 Msa1034890:three_prime_UTR 35.0%
! CATGACAAATTTGACTTTGG+AGG + chr7_1:70604610-70604629 Msa1034890:three_prime_UTR 35.0%
GTGATTTCGTTTGACTGAAA+AGG - chr7_1:70604645-70604664 None:intergenic 35.0%
GTCAAACGAAATCACATGTA+AGG + chr7_1:70604650-70604669 Msa1034890:three_prime_UTR 35.0%
CTTCCTCAATTCAACAACCA+TGG + chr7_1:70601081-70601100 Msa1034890:exon 40.0%
GATATCTTCGTAGTCATCGT+CGG - chr7_1:70601107-70601126 None:intergenic 40.0%
TGACTACGAAGATATCGACA+TGG + chr7_1:70601111-70601130 Msa1034890:CDS 40.0%
GACTACGAAGATATCGACAT+GGG + chr7_1:70601112-70601131 Msa1034890:CDS 40.0%
ACTACGAAGATATCGACATG+GGG + chr7_1:70601113-70601132 Msa1034890:CDS 40.0%
ATTAAAACGATGCAGGTACG+AGG + chr7_1:70601208-70601227 Msa1034890:intron 40.0%
! TTGATGATTGATGCATGTGC+AGG + chr7_1:70601303-70601322 Msa1034890:intron 40.0%
AATGATGACCTAGATGCAGA+AGG + chr7_1:70601358-70601377 Msa1034890:CDS 40.0%
AGGAGAACCAATTGAAACTG+AGG + chr7_1:70601378-70601397 Msa1034890:CDS 40.0%
CATATACTTGGAAGTCCTTC+TGG - chr7_1:70601434-70601453 None:intergenic 40.0%
! TAAACCTGATTTGAAGAGCG+CGG - chr7_1:70601489-70601508 None:intergenic 40.0%
GTAAAACAGGACAAACTGGA+AGG - chr7_1:70601619-70601638 None:intergenic 40.0%
! TTTCCTCCACGAATTTTCGT+TGG + chr7_1:70601828-70601847 Msa1034890:intron 40.0%
AGACATCCAACGAAAATTCG+TGG - chr7_1:70601837-70601856 None:intergenic 40.0%
GAGGATTGCATTATGAGACA+AGG + chr7_1:70602046-70602065 Msa1034890:intron 40.0%
TGAACATAGATCACAGCCAT+AGG + chr7_1:70602126-70602145 Msa1034890:intron 40.0%
ACACAGAAGCTGAAATCCTA+TGG - chr7_1:70602145-70602164 None:intergenic 40.0%
ACTCCAGTGGTTTCTAATCT+AGG - chr7_1:70602178-70602197 None:intergenic 40.0%
! AACCACTGGAGTTTTCTTCA+TGG + chr7_1:70602186-70602205 Msa1034890:intron 40.0%
! GGATTTTTGTGATGCAGACT+AGG - chr7_1:70602242-70602261 None:intergenic 40.0%
CGAATACTCAGGTATCTTAG+AGG - chr7_1:70602333-70602352 None:intergenic 40.0%
TGGACAACAAAATAGGTTGC+AGG + chr7_1:70602409-70602428 Msa1034890:intron 40.0%
GCTTGACAGTTAGGTTAAGT+TGG - chr7_1:70602545-70602564 None:intergenic 40.0%
ACAATGACTAAATGCCCGTT+TGG + chr7_1:70602678-70602697 Msa1034890:intron 40.0%
GGGGAGAATTTAAACCAAAC+GGG - chr7_1:70602695-70602714 None:intergenic 40.0%
TACAAGCTATGGACAATGGT+TGG - chr7_1:70603400-70603419 None:intergenic 40.0%
! GTCCATAGCTTGTACTTTCT+TGG + chr7_1:70603406-70603425 Msa1034890:intron 40.0%
! AGCCAAGAAAGTACAAGCTA+TGG - chr7_1:70603411-70603430 None:intergenic 40.0%
! TAGCTTGTACTTTCTTGGCT+CGG + chr7_1:70603411-70603430 Msa1034890:intron 40.0%
TTCATGGCAATCTGAAGAGT+TGG - chr7_1:70603951-70603970 None:intergenic 40.0%
AACTCTTCAGATTGCCATGA+AGG + chr7_1:70603950-70603969 Msa1034890:intron 40.0%
! GGGTACTTCTTTTTCTTGGA+TGG + chr7_1:70604056-70604075 Msa1034890:intron 40.0%
! ACTTCTTTTTCTTGGATGGG+AGG + chr7_1:70604060-70604079 Msa1034890:intron 40.0%
! CTTCTTTTTCTTGGATGGGA+GGG + chr7_1:70604061-70604080 Msa1034890:intron 40.0%
TAGCTGGACAATTGCCAAAA+TGG - chr7_1:70604128-70604147 None:intergenic 40.0%
TTGTCCAGCTATGAAGATTG+GGG + chr7_1:70604137-70604156 Msa1034890:intron 40.0%
ATTGCTTGAACTGCTAGTGT+GGG + chr7_1:70604214-70604233 Msa1034890:exon 40.0%
CTATGCTCCTACCATAAAAC+TGG + chr7_1:70604301-70604320 Msa1034890:three_prime_UTR 40.0%
TGTTTGTCCAATCAACTGTC+AGG + chr7_1:70604454-70604473 Msa1034890:three_prime_UTR 40.0%
TGATCTTCCAAAAAGAACCG+AGG + chr7_1:70604586-70604605 Msa1034890:three_prime_UTR 40.0%
TGATTGATGCATGTGCAGGA+AGG + chr7_1:70601307-70601326 Msa1034890:intron 45.0%
! TTGGTTCTCCTTCTGCATCT+AGG - chr7_1:70601369-70601388 None:intergenic 45.0%
TGACTAAGTATGAGCGTGCT+AGG + chr7_1:70601452-70601471 Msa1034890:CDS 45.0%
TATGAGCGTGCTAGGATTCT+CGG + chr7_1:70601460-70601479 Msa1034890:CDS 45.0%
! AAACCTGATTTGAAGAGCGC+GGG - chr7_1:70601488-70601507 None:intergenic 45.0%
! CATTGCTCTTGCTTGCCTTT+TGG - chr7_1:70601663-70601682 None:intergenic 45.0%
AGTAAGGATGAGAAAGAGGC+TGG - chr7_1:70601707-70601726 None:intergenic 45.0%
CATCCAACGAAAATTCGTGG+AGG - chr7_1:70601834-70601853 None:intergenic 45.0%
ACCATCCTGAGTTTGTCTCT+AGG + chr7_1:70601873-70601892 Msa1034890:intron 45.0%
ACCTAGAGACAAACTCAGGA+TGG - chr7_1:70601877-70601896 None:intergenic 45.0%
CACAACCTAGAGACAAACTC+AGG - chr7_1:70601881-70601900 None:intergenic 45.0%
TCTCTAGGTTGTGAAACGCA+GGG + chr7_1:70601888-70601907 Msa1034890:intron 45.0%
!! TCTACATACGTGTCGGTTGT+TGG + chr7_1:70601940-70601959 Msa1034890:intron 45.0%
AGTTGTCAACTCCACTTGCA+TGG + chr7_1:70602013-70602032 Msa1034890:intron 45.0%
CCACAAAAGAACCATGCAAG+TGG - chr7_1:70602027-70602046 None:intergenic 45.0%
! CCACTTGCATGGTTCTTTTG+TGG + chr7_1:70602024-70602043 Msa1034890:intron 45.0%
! CTTGCATGGTTCTTTTGTGG+AGG + chr7_1:70602027-70602046 Msa1034890:intron 45.0%
CTGCCTAGATTAGAAACCAC+TGG + chr7_1:70602172-70602191 Msa1034890:intron 45.0%
GTCCATGAAGAAAACTCCAG+TGG - chr7_1:70602191-70602210 None:intergenic 45.0%
GACAGAACCAGCATCTATTC+TGG - chr7_1:70602263-70602282 None:intergenic 45.0%
!! GAATAGATGCTGGTTCTGTC+AGG + chr7_1:70602263-70602282 Msa1034890:intron 45.0%
! TGTCAGGTTTTCCGTTCTGT+AGG + chr7_1:70602279-70602298 Msa1034890:intron 45.0%
CATGGTTCACTCCTACAGAA+CGG - chr7_1:70602293-70602312 None:intergenic 45.0%
!! CTTAGAGGCACTATCACTCA+AGG - chr7_1:70602318-70602337 None:intergenic 45.0%
! AATAGGTTGCAGGCCTTGTA+AGG + chr7_1:70602419-70602438 Msa1034890:intron 45.0%
ATGGGGGAAAACACTTACCA+AGG + chr7_1:70602727-70602746 Msa1034890:intron 45.0%
CAGTATGAATGCACCTGTCA+TGG + chr7_1:70602874-70602893 Msa1034890:intron 45.0%
AGCTTGTACTTTCTTGGCTC+GGG + chr7_1:70603412-70603431 Msa1034890:intron 45.0%
AAGTAGCGACGGATGGTAAA+AGG - chr7_1:70603996-70604015 None:intergenic 45.0%
TTTCTTGGATGGGAGGGTTA+AGG + chr7_1:70604067-70604086 Msa1034890:intron 45.0%
AACTCCCCAATCTTCATAGC+TGG - chr7_1:70604144-70604163 None:intergenic 45.0%
GATTGCTTGAACTGCTAGTG+TGG + chr7_1:70604213-70604232 Msa1034890:exon 45.0%
TTGCTTGAACTGCTAGTGTG+GGG + chr7_1:70604215-70604234 Msa1034890:three_prime_UTR 45.0%
AGTGCATCCTGACAGTTGAT+TGG - chr7_1:70604464-70604483 None:intergenic 45.0%
! GTCATGTCCTCGGTTCTTTT+TGG - chr7_1:70604596-70604615 None:intergenic 45.0%
!! ACATAATTAATAATTAATTA+GGG + chr7_1:70601163-70601182 Msa1034890:intron 5.0%
CGGCCATGGTTGTTGAATTG+AGG - chr7_1:70601087-70601106 None:intergenic 50.0%
AAAACGATGCAGGTACGAGG+AGG + chr7_1:70601211-70601230 Msa1034890:intron 50.0%
! ACCTCAATTTCAGGCTCTGG+TGG - chr7_1:70601239-70601258 None:intergenic 50.0%
GGTACCTCAATTTCAGGCTC+TGG - chr7_1:70601242-70601261 None:intergenic 50.0%
GGAAAAGGAAGATGCTGCTC+CGG + chr7_1:70601399-70601418 Msa1034890:CDS 50.0%
TGTGTATCCCTAACCACCGA+TGG + chr7_1:70601681-70601700 Msa1034890:intron 50.0%
! ATGAGAAAGAGGCTGGCCAT+CGG - chr7_1:70601700-70601719 None:intergenic 50.0%
GTCTCTAGGTTGTGAAACGC+AGG + chr7_1:70601887-70601906 Msa1034890:intron 50.0%
CTCTAGGTTGTGAAACGCAG+GGG + chr7_1:70601889-70601908 Msa1034890:intron 50.0%
CGTTCTGTAGGAGTGAACCA+TGG + chr7_1:70602291-70602310 Msa1034890:intron 50.0%
GCACTATCACTCAAGGACCA+TGG - chr7_1:70602311-70602330 None:intergenic 50.0%
!! GCTTGGTGCAATCTCTAAGC+AGG - chr7_1:70602488-70602507 None:intergenic 50.0%
! TCTCCCCTCACTGACTACTA+TGG + chr7_1:70602708-70602727 Msa1034890:intron 50.0%
! CTCCCCTCACTGACTACTAT+GGG + chr7_1:70602709-70602728 Msa1034890:intron 50.0%
! TCCCCTCACTGACTACTATG+GGG + chr7_1:70602710-70602729 Msa1034890:intron 50.0%
TCCCCCATAGTAGTCAGTGA+GGG - chr7_1:70602715-70602734 None:intergenic 50.0%
TTCCCCCATAGTAGTCAGTG+AGG - chr7_1:70602716-70602735 None:intergenic 50.0%
TGCACCTGTCATGGTTGAGT+TGG + chr7_1:70602883-70602902 Msa1034890:CDS 50.0%
!! ACCTGTCATGGTTGAGTTGG+AGG + chr7_1:70602886-70602905 Msa1034890:CDS 50.0%
!! CCTGTCATGGTTGAGTTGGA+GGG + chr7_1:70602887-70602906 Msa1034890:CDS 50.0%
!! CTGTCATGGTTGAGTTGGAG+GGG + chr7_1:70602888-70602907 Msa1034890:CDS 50.0%
!! TGTCATGGTTGAGTTGGAGG+GGG + chr7_1:70602889-70602908 Msa1034890:CDS 50.0%
!! TTGTTGTGTTGGCTCTGCCA+TGG + chr7_1:70603860-70603879 Msa1034890:intron 50.0%
TTCCATCAGGCAAGTAGCGA+CGG - chr7_1:70604007-70604026 None:intergenic 50.0%
!! GGGAGTTGATGAGCTGATTG+TGG + chr7_1:70604157-70604176 Msa1034890:CDS 50.0%
AGCTGATTGTGGAAGACTCC+TGG + chr7_1:70604168-70604187 Msa1034890:CDS 50.0%
AGACTCCTGGAAGAGACAAG+TGG + chr7_1:70604181-70604200 Msa1034890:CDS 50.0%
GACTCCTGGAAGAGACAAGT+GGG + chr7_1:70604182-70604201 Msa1034890:CDS 50.0%
CAACTGTCAGGATGCACTAC+TGG + chr7_1:70604466-70604485 Msa1034890:three_prime_UTR 50.0%
AGGCATTCATTCCGTCCGAG+TGG + chr7_1:70601031-70601050 Msa1034890:five_prime_UTR 55.0%
GGCATTCATTCCGTCCGAGT+GGG + chr7_1:70601032-70601051 Msa1034890:five_prime_UTR 55.0%
GCATTCATTCCGTCCGAGTG+GGG + chr7_1:70601033-70601052 Msa1034890:five_prime_UTR 55.0%
CATTCATTCCGTCCGAGTGG+GGG + chr7_1:70601034-70601053 Msa1034890:five_prime_UTR 55.0%
ATTCATTCCGTCCGAGTGGG+GGG + chr7_1:70601035-70601054 Msa1034890:five_prime_UTR 55.0%
! TCTAAAACCCCCCACTCGGA+CGG - chr7_1:70601045-70601064 None:intergenic 55.0%
!! CTGCTCTAAAACCCCCCACT+CGG - chr7_1:70601049-70601068 None:intergenic 55.0%
!!! GGGGTTTTAGAGCAGAGCAG+AGG + chr7_1:70601054-70601073 Msa1034890:five_prime_UTR 55.0%
TTCGTAGTCATCGTCGGCCA+TGG - chr7_1:70601101-70601120 None:intergenic 55.0%
GCCACCAGAGCCTGAAATTG+AGG + chr7_1:70601235-70601254 Msa1034890:CDS 55.0%
!! GTCCTTCTGGGACGTTCAAC+CGG - chr7_1:70601421-70601440 None:intergenic 55.0%
GTACCCGCGCTCTTCAAATC+AGG + chr7_1:70601482-70601501 Msa1034890:CDS 55.0%
CCCCCATAGTAGTCAGTGAG+GGG - chr7_1:70602714-70602733 None:intergenic 55.0%
! CCCCTCACTGACTACTATGG+GGG + chr7_1:70602711-70602730 Msa1034890:intron 55.0%
CCCTCCAACTCAACCATGAC+AGG - chr7_1:70602890-70602909 None:intergenic 55.0%
CCATGAAGGAGCTACGAGAG+CGG + chr7_1:70603964-70603983 Msa1034890:CDS 55.0%
ATCAGGCAAGTAGCGACGGA+TGG - chr7_1:70604003-70604022 None:intergenic 55.0%
ATCCGTCGCTACTTGCCTGA+TGG + chr7_1:70604002-70604021 Msa1034890:CDS 55.0%
GTCGCTACTTGCCTGATGGA+AGG + chr7_1:70604006-70604025 Msa1034890:CDS 55.0%
TCCTGGAAGAGACAAGTGGG+TGG + chr7_1:70604185-70604204 Msa1034890:CDS 55.0%
ACCACCCACTTGTCTCTTCC+AGG - chr7_1:70604189-70604208 None:intergenic 55.0%
! GCATGTGCAGGAAGGTGCAG+AGG + chr7_1:70601315-70601334 Msa1034890:intron 60.0%
! TGTGCAGGAAGGTGCAGAGG+AGG + chr7_1:70601318-70601337 Msa1034890:intron 60.0%
CTCCGGTTGAACGTCCCAGA+AGG + chr7_1:70601416-70601435 Msa1034890:CDS 60.0%
! AGGCTGGCCATCGGTGGTTA+GGG - chr7_1:70601691-70601710 None:intergenic 60.0%
! AGAAAGAGGCTGGCCATCGG+TGG - chr7_1:70601697-70601716 None:intergenic 60.0%
GGGGGAAACTGACCCACTTG+AGG + chr7_1:70602907-70602926 Msa1034890:CDS 60.0%
CCGCTCTCGTAGCTCCTTCA+TGG - chr7_1:70603967-70603986 None:intergenic 60.0%
! GCAGGAAGGTGCAGAGGAGG+AGG + chr7_1:70601321-70601340 Msa1034890:intron 65.0%
! GAGGCTGGCCATCGGTGGTT+AGG - chr7_1:70601692-70601711 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr7_1 gene 70601008 70604684 70601008 ID=Msa1034890;Name=Msa1034890
chr7_1 mRNA 70601008 70604684 70601008 ID=Msa1034890-mRNA-1;Parent=Msa1034890;Name=Msa1034890-mRNA-1;_AED=0.06;_eAED=0.06;_QI=92|1|1|1|1|1|6|470|145
chr7_1 exon 70601008 70601134 70601008 ID=Msa1034890-mRNA-1:exon:16908;Parent=Msa1034890-mRNA-1
chr7_1 exon 70601223 70601256 70601223 ID=Msa1034890-mRNA-1:exon:16909;Parent=Msa1034890-mRNA-1
chr7_1 exon 70601325 70601503 70601325 ID=Msa1034890-mRNA-1:exon:16910;Parent=Msa1034890-mRNA-1
chr7_1 exon 70602877 70602928 70602877 ID=Msa1034890-mRNA-1:exon:16911;Parent=Msa1034890-mRNA-1
chr7_1 exon 70603960 70604027 70603960 ID=Msa1034890-mRNA-1:exon:16912;Parent=Msa1034890-mRNA-1
chr7_1 exon 70604145 70604684 70604145 ID=Msa1034890-mRNA-1:exon:16913;Parent=Msa1034890-mRNA-1
chr7_1 five_prime_UTR 70601008 70601099 70601008 ID=Msa1034890-mRNA-1:five_prime_utr;Parent=Msa1034890-mRNA-1
chr7_1 CDS 70601100 70601134 70601100 ID=Msa1034890-mRNA-1:cds;Parent=Msa1034890-mRNA-1
chr7_1 CDS 70601223 70601256 70601223 ID=Msa1034890-mRNA-1:cds;Parent=Msa1034890-mRNA-1
chr7_1 CDS 70601325 70601503 70601325 ID=Msa1034890-mRNA-1:cds;Parent=Msa1034890-mRNA-1
chr7_1 CDS 70602877 70602928 70602877 ID=Msa1034890-mRNA-1:cds;Parent=Msa1034890-mRNA-1
chr7_1 CDS 70603960 70604027 70603960 ID=Msa1034890-mRNA-1:cds;Parent=Msa1034890-mRNA-1
chr7_1 CDS 70604145 70604214 70604145 ID=Msa1034890-mRNA-1:cds;Parent=Msa1034890-mRNA-1
chr7_1 three_prime_UTR 70604215 70604684 70604215 ID=Msa1034890-mRNA-1:three_prime_utr;Parent=Msa1034890-mRNA-1
Gene Sequence

>Msa1034890

ATGGCCGACGATGACTACGAAGATATCGACATGGGGTACGAGGAGGAGCCACCAGAGCCTGAAATTGAGGAAGGTGCAGAGGAGGAGGATCCTGATAACAAGAATGATGACCTAGATGCAGAAGGAGAACCAATTGAAACTGAGGAAAAGGAAGATGCTGCTCCGGTTGAACGTCCCAGAAGGACTTCCAAGTATATGACTAAGTATGAGCGTGCTAGGATTCTCGGTACCCGCGCTCTTCAAATCAGTATGAATGCACCTGTCATGGTTGAGTTGGAGGGGGAAACTGACCCACTTGAGATTGCCATGAAGGAGCTACGAGAGCGGAAAATTCCTTTTACCATCCGTCGCTACTTGCCTGATGGAAGCTATGAAGATTGGGGAGTTGATGAGCTGATTGTGGAAGACTCCTGGAAGAGACAAGTGGGTGGTGATTGA
Protein sequence

>Msa1034890

MADDDYEDIDMGYEEEPPEPEIEEGAEEEDPDNKNDDLDAEGEPIETEEKEDAAPVERPRRTSKYMTKYERARILGTRALQISMNAPVMVELEGETDPLEIAMKELRERKIPFTIRRYLPDGSYEDWGVDELIVEDSWKRQVGGD*