AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1036820

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1036820 MtrunA17_Chr7g0261381 97.674 215 2 3 1 213 1 214 3.78e-154 426
Msa1036820 MtrunA17_Chr1g0167411 75.132 189 46 1 1 188 1 189 4.63e-94 273
Msa1036820 MtrunA17_Chr1g0183761 100.000 131 0 0 64 194 45 175 3.67e-87 256
Msa1036820 MtrunA17_Chr4g0051251 82.609 138 24 0 63 200 45 182 2.18e-85 252
Msa1036820 MtrunA17_Chr1g0187671 81.818 165 30 0 30 194 19 183 2.72e-85 251
Msa1036820 MtrunA17_Chr5g0431211 80.451 133 26 0 63 195 36 168 1.37e-82 245
Msa1036820 MtrunA17_Chr3g0105921 81.818 132 24 0 64 195 42 173 1.13e-73 222
Msa1036820 MtrunA17_Chr2g0325831 71.724 145 34 1 43 187 9 146 4.04e-73 219
Msa1036820 MtrunA17_Chr7g0274631 65.432 162 48 3 35 195 4 158 4.68e-72 216
Msa1036820 MtrunA17_Chr4g0041141 65.333 150 52 0 38 187 3 152 5.41e-71 214
Msa1036820 MtrunA17_Chr2g0283861 75.758 132 32 0 63 194 34 165 1.02e-70 213
Msa1036820 MtrunA17_Chr2g0315791 75.000 132 33 0 63 194 32 163 4.97e-70 211
Msa1036820 MtrunA17_Chr1g0190231 71.250 160 46 0 29 188 5 164 1.69e-69 210
Msa1036820 MtrunA17_Chr8g0379061 72.800 125 34 0 63 187 84 208 3.14e-55 176
Msa1036820 MtrunA17_Chr1g0211641 48.624 109 29 2 21 102 1 109 7.00e-24 91.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1036820 AT2G31160.1 80.814 172 30 1 26 197 19 187 4.89e-88 259
Msa1036820 AT1G07090.1 76.389 144 34 0 53 196 20 163 1.96e-84 249
Msa1036820 AT3G23290.2 71.809 188 37 2 1 188 1 172 5.64e-80 237
Msa1036820 AT5G58500.1 79.104 134 27 1 63 195 18 151 7.09e-79 234
Msa1036820 AT2G42610.1 75.714 140 34 0 59 198 20 159 6.85e-74 221
Msa1036820 AT2G42610.2 75.714 140 34 0 59 198 20 159 6.85e-74 221
Msa1036820 AT5G28490.1 83.088 136 23 0 58 193 19 154 1.28e-72 219
Msa1036820 AT3G04510.1 80.000 130 26 0 64 193 33 162 1.53e-69 211
Msa1036820 AT1G78815.1 67.200 125 41 0 63 187 39 163 1.27e-62 193
Msa1036820 AT4G18610.1 69.048 126 38 1 63 187 36 161 4.51e-62 192
Msa1036820 AT1G16910.1 61.194 134 52 0 59 192 18 151 2.81e-58 181

Find 84 sgRNAs with CRISPR-Local

Find 246 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATCATGGTGTTTAGGTATT+TGG 0.100645 7_1:+73264449 Msa1036820:intron
TTTAGGTATTTGGATCAATT+TGG 0.178063 7_1:+73264459 Msa1036820:intron
GCACCAAAAGGATTTGTTTC+TGG 0.187984 7_1:-73264630 None:intergenic
AATTAATACCTTGTTCTTCT+AGG 0.210004 7_1:-73263700 None:intergenic
CATATAGAGTGCTTTGTCTT+TGG 0.231876 7_1:+73264887 Msa1036820:three_prime_UTR
CTTCCCCATGCTTGCCTTAG+AGG 0.241570 7_1:-73264552 None:intergenic
CTAAAATCTAGTATGATTAA+AGG 0.244347 7_1:-73265208 None:intergenic
TCGATGTGGATAACATTATT+TGG 0.250214 7_1:+73265344 Msa1036820:three_prime_UTR
GATTCAATTCAAGAATTTAT+AGG 0.256232 7_1:+73263825 Msa1036820:CDS
AAGAACAAGGTATTAATTCT+TGG 0.280943 7_1:+73263705 Msa1036820:five_prime_UTR
GTTGTTATTCATGAAGTTAC+AGG 0.282953 7_1:-73263871 None:intergenic
GGACATGGTGCTGGTGGATT+TGG 0.312240 7_1:-73264525 None:intergenic
ATTCAATTCAAGAATTTATA+GGG 0.321871 7_1:+73263826 Msa1036820:CDS
TGTTAGGCTTTGAAGGAAAT+AGG 0.324854 7_1:-73263310 None:intergenic
AGAGAGGTGTTAGGCTTTGA+AGG 0.331061 7_1:-73263317 None:intergenic
GGATTTGGATGCCCATAAAA+TGG 0.348197 7_1:-73264510 None:intergenic
TTAGGTATTTGGATCAATTT+GGG 0.353257 7_1:+73264460 Msa1036820:intron
CACCATGTCCATGTCCTCTA+AGG 0.357440 7_1:+73264538 Msa1036820:CDS
CCTATCAATATATACACATA+TGG 0.366144 7_1:-73265375 None:intergenic
CCATATGTGTATATATTGAT+AGG 0.371923 7_1:+73265375 Msa1036820:three_prime_UTR
CGCCGTGACTGGAACACCTT+TGG 0.383976 7_1:+73264029 Msa1036820:CDS
ATATTAATATTCTTCATTCA+TGG 0.385287 7_1:-73263368 None:intergenic
TGCCCATAAAATGGACAAAT+TGG 0.388714 7_1:-73264501 None:intergenic
GAAAAGTTGGACAGTAGGTT+TGG 0.403335 7_1:-73263284 None:intergenic
CTCATAGCGGCTGCTACTGT+TGG 0.404554 7_1:-73263997 None:intergenic
AGGAAATAGGAAAGAAAAGT+TGG 0.404681 7_1:-73263297 None:intergenic
TTCTGTCACAGCAAGTAAAC+AGG 0.405995 7_1:-73264912 None:intergenic
AAACCAGAAACAAATCCTTT+TGG 0.416713 7_1:+73264627 Msa1036820:CDS
GAACTTTGAAGCTACATTTG+TGG 0.427671 7_1:+73265295 Msa1036820:three_prime_UTR
ATTAATACCTTGTTCTTCTA+GGG 0.448154 7_1:-73263699 None:intergenic
TCCACATCGATCACTATGTA+TGG 0.449619 7_1:-73265331 None:intergenic
AGAATCAGAAACGCCGTGAC+TGG 0.455928 7_1:+73264018 Msa1036820:CDS
GTTGCACTTGCACCTTGTGC+TGG 0.465381 7_1:-73264771 None:intergenic
ACTGTTGGGACTGTTGATGA+TGG 0.472335 7_1:-73263982 None:intergenic
CTTGCACCTTGTGCTGGTGG+TGG 0.478161 7_1:-73264765 None:intergenic
ACATATACCTTAGGAATTCA+AGG 0.481244 7_1:-73264112 None:intergenic
GGAAAGAAAAGTTGGACAGT+AGG 0.482092 7_1:-73263289 None:intergenic
CACAAAATTCAAAACAACCT+TGG 0.482839 7_1:+73263752 Msa1036820:five_prime_UTR
AGTTGGACAGTAGGTTTGGA+TGG 0.483212 7_1:-73263280 None:intergenic
TCATAGCGGCTGCTACTGTT+GGG 0.492824 7_1:-73263996 None:intergenic
GGTGGAACTTGTTGTTGTTG+TGG 0.502983 7_1:-73264747 None:intergenic
TCATCACTTGTTAGTGCTAG+TGG 0.505405 7_1:-73263393 None:intergenic
CTTTGTGCTTTGCAGATCCA+AGG 0.509553 7_1:-73263769 None:intergenic
ACTTGTTGTTGTTGTGGTGG+TGG 0.511066 7_1:-73264741 None:intergenic
CTCACAGCTCGAGCACCAAA+AGG 0.515193 7_1:-73264642 None:intergenic
CACATGTCCTTGAATTCCTA+AGG 0.521843 7_1:+73264105 Msa1036820:CDS
GGAAGCCTTGATGCACTCAT+AGG 0.523874 7_1:+73264570 Msa1036820:CDS
CATGTCCTCTAAGGCAAGCA+TGG 0.527761 7_1:+73264547 Msa1036820:CDS
CAGTAGGTTTGGATGGAGAC+AGG 0.529323 7_1:-73263273 None:intergenic
CATGCTTGCCTTAGAGGACA+TGG 0.533497 7_1:-73264546 None:intergenic
GGAACTTGTTGTTGTTGTGG+TGG 0.534959 7_1:-73264744 None:intergenic
GCACTTGCACCTTGTGCTGG+TGG 0.535986 7_1:-73264768 None:intergenic
CTATCAATATATACACATAT+GGG 0.537454 7_1:-73265374 None:intergenic
AGAGAATGTGATGAGATTGA+TGG 0.544970 7_1:-73263547 None:intergenic
GTCCACCTCTTTCCCTCTCT+AGG 0.545320 7_1:+73264072 Msa1036820:CDS
AGAGGACATGGACATGGTGC+TGG 0.548410 7_1:-73264534 None:intergenic
ATGTCCTCTAAGGCAAGCAT+GGG 0.548889 7_1:+73264548 Msa1036820:CDS
TTGAAGCTTGTGAATATACA+TGG 0.551801 7_1:+73265037 Msa1036820:three_prime_UTR
TGTTGTTGTTAAGCTTGTTG+TGG 0.560960 7_1:-73263919 None:intergenic
GACCAAAGGTGTTCCAGTCA+CGG 0.570044 7_1:-73264031 None:intergenic
TACTGGTAGTAGACTCATAG+TGG 0.572208 7_1:+73265005 Msa1036820:three_prime_UTR
AGACAGTGCTGTGTTTGTAC+TGG 0.573730 7_1:+73264988 Msa1036820:three_prime_UTR
CTGCACCTAGAGAGGGAAAG+AGG 0.576931 7_1:-73264077 None:intergenic
GATCTTCAATCAAAAGCAAG+AGG 0.593770 7_1:+73264687 Msa1036820:CDS
GCTTCCCAAAACTATAGCTG+TGG 0.599047 7_1:-73263480 None:intergenic
TCATAGCTGTTATTGTTGAG+TGG 0.610117 7_1:-73265096 None:intergenic
GAGAATGTGATGAGATTGAT+GGG 0.614654 7_1:-73263546 None:intergenic
GTGCACCGCTGCACCTAGAG+AGG 0.622701 7_1:-73264085 None:intergenic
CTTTCCCTCTCTAGGTGCAG+CGG 0.626380 7_1:+73264080 Msa1036820:CDS
CAAGGAACCCTAGAAGAACA+AGG 0.629527 7_1:+73263692 Msa1036820:intron
GACGACCTATGAGTGCATCA+AGG 0.630031 7_1:-73264575 None:intergenic
CAAGTTCCACCACCAGCACA+AGG 0.630971 7_1:+73264759 Msa1036820:CDS
GATGGTTGAAGCTGCAGATG+AGG 0.631628 7_1:-73263964 None:intergenic
TGCCTTAGAGGACATGGACA+TGG 0.634202 7_1:-73264540 None:intergenic
CTTGGATCTGCAAAGCACAA+AGG 0.637595 7_1:+73263770 Msa1036820:five_prime_UTR
TGCACCGCTGCACCTAGAGA+GGG 0.638717 7_1:-73264084 None:intergenic
GGACATGGACATGGTGCTGG+TGG 0.656231 7_1:-73264531 None:intergenic
GAAGATCACGAACTTCGCGA+AGG 0.664694 7_1:-73264671 None:intergenic
GGCGTTTCTGATTCTCATAG+CGG 0.670856 7_1:-73264010 None:intergenic
ATTCTTGAGATACTGACCAA+AGG 0.683345 7_1:-73264045 None:intergenic
CACCTAGAGAGGGAAAGAGG+TGG 0.686727 7_1:-73264074 None:intergenic
TCCATACATAGTGATCGATG+TGG 0.693643 7_1:+73265330 Msa1036820:three_prime_UTR
TGTCCTCTAAGGCAAGCATG+GGG 0.718093 7_1:+73264549 Msa1036820:CDS
GCTGAGAAATGAGAATCACA+CGG 0.732864 7_1:-73265070 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ACTATTTTTATGTTTATTTT+AGG - chr7_1:73263634-73263653 None:intergenic 10.0%
!!! ACTTTTTTTTAATCATATTT+AGG + chr7_1:73264831-73264850 Msa1036820:three_prime_UTR 10.0%
!!! CTTTTTTTTAATCATATTTA+GGG + chr7_1:73264832-73264851 Msa1036820:three_prime_UTR 10.0%
!!! ACTATTTTTATGTTTATTTT+AGG - chr7_1:73263634-73263653 None:intergenic 10.0%
!!! ACTTTTTTTTAATCATATTT+AGG + chr7_1:73264831-73264850 Msa1036820:three_prime_UTR 10.0%
!!! CTTTTTTTTAATCATATTTA+GGG + chr7_1:73264832-73264851 Msa1036820:three_prime_UTR 10.0%
!! ATATTAATATTCTTCATTCA+TGG - chr7_1:73263371-73263390 None:intergenic 15.0%
!!! TAGTTTTTATTTCCTTAAAA+GGG - chr7_1:73263450-73263469 None:intergenic 15.0%
!!! TTTATGTTTATTTTAGGAAT+AGG - chr7_1:73263628-73263647 None:intergenic 15.0%
!! ATTCAATTCAAGAATTTATA+GGG + chr7_1:73263826-73263845 Msa1036820:CDS 15.0%
!!! TTATTTTGGTTAATTTATCA+TGG + chr7_1:73264434-73264453 Msa1036820:intron 15.0%
!! ATATTAATATTCTTCATTCA+TGG - chr7_1:73263371-73263390 None:intergenic 15.0%
!!! TAGTTTTTATTTCCTTAAAA+GGG - chr7_1:73263450-73263469 None:intergenic 15.0%
!!! TTTATGTTTATTTTAGGAAT+AGG - chr7_1:73263628-73263647 None:intergenic 15.0%
!! ATTCAATTCAAGAATTTATA+GGG + chr7_1:73263826-73263845 Msa1036820:CDS 15.0%
!!! TTATTTTGGTTAATTTATCA+TGG + chr7_1:73264434-73264453 Msa1036820:intron 15.0%
!!! GTAGTTTTTATTTCCTTAAA+AGG - chr7_1:73263451-73263470 None:intergenic 20.0%
!!! AAGTGAAAGTGTTAATTAAT+TGG - chr7_1:73263667-73263686 None:intergenic 20.0%
!!! AAAGTTAAGCTATATTTTCA+TGG + chr7_1:73263803-73263822 Msa1036820:exon 20.0%
!! GATTCAATTCAAGAATTTAT+AGG + chr7_1:73263825-73263844 Msa1036820:CDS 20.0%
!!! TGCATCACAATTTTTTATAT+CGG + chr7_1:73264234-73264253 Msa1036820:intron 20.0%
!! CTAAAATCTAGTATGATTAA+AGG - chr7_1:73265211-73265230 None:intergenic 20.0%
!!! CTTTAATCATACTAGATTTT+AGG + chr7_1:73265209-73265228 Msa1036820:three_prime_UTR 20.0%
!! CTATCAATATATACACATAT+GGG - chr7_1:73265377-73265396 None:intergenic 20.0%
!!! GTAGTTTTTATTTCCTTAAA+AGG - chr7_1:73263451-73263470 None:intergenic 20.0%
!!! AAGTGAAAGTGTTAATTAAT+TGG - chr7_1:73263667-73263686 None:intergenic 20.0%
!!! AAAGTTAAGCTATATTTTCA+TGG + chr7_1:73263803-73263822 Msa1036820:exon 20.0%
!! GATTCAATTCAAGAATTTAT+AGG + chr7_1:73263825-73263844 Msa1036820:CDS 20.0%
!!! TGCATCACAATTTTTTATAT+CGG + chr7_1:73264234-73264253 Msa1036820:intron 20.0%
!! CTAAAATCTAGTATGATTAA+AGG - chr7_1:73265211-73265230 None:intergenic 20.0%
!!! CTTTAATCATACTAGATTTT+AGG + chr7_1:73265209-73265228 Msa1036820:three_prime_UTR 20.0%
!! CTATCAATATATACACATAT+GGG - chr7_1:73265377-73265396 None:intergenic 20.0%
! ATTAATACCTTGTTCTTCTA+GGG - chr7_1:73263702-73263721 None:intergenic 25.0%
! AATTAATACCTTGTTCTTCT+AGG - chr7_1:73263703-73263722 None:intergenic 25.0%
!! AAGAACAAGGTATTAATTCT+TGG + chr7_1:73263705-73263724 Msa1036820:five_prime_UTR 25.0%
! ATTTGTACTACATATACCTT+AGG - chr7_1:73264124-73264143 None:intergenic 25.0%
! GTCATTATATAACTCAATTG+CGG + chr7_1:73264371-73264390 Msa1036820:intron 25.0%
! GTTAATTTATCATGGTGTTT+AGG + chr7_1:73264442-73264461 Msa1036820:intron 25.0%
! TTTAGGTATTTGGATCAATT+TGG + chr7_1:73264459-73264478 Msa1036820:intron 25.0%
! TTAGGTATTTGGATCAATTT+GGG + chr7_1:73264460-73264479 Msa1036820:intron 25.0%
! CCTATCAATATATACACATA+TGG - chr7_1:73265378-73265397 None:intergenic 25.0%
! CCATATGTGTATATATTGAT+AGG + chr7_1:73265375-73265394 Msa1036820:three_prime_UTR 25.0%
! ATTAATACCTTGTTCTTCTA+GGG - chr7_1:73263702-73263721 None:intergenic 25.0%
! AATTAATACCTTGTTCTTCT+AGG - chr7_1:73263703-73263722 None:intergenic 25.0%
!! AAGAACAAGGTATTAATTCT+TGG + chr7_1:73263705-73263724 Msa1036820:five_prime_UTR 25.0%
! ATTTGTACTACATATACCTT+AGG - chr7_1:73264124-73264143 None:intergenic 25.0%
! GTCATTATATAACTCAATTG+CGG + chr7_1:73264371-73264390 Msa1036820:intron 25.0%
! GTTAATTTATCATGGTGTTT+AGG + chr7_1:73264442-73264461 Msa1036820:intron 25.0%
! TTTAGGTATTTGGATCAATT+TGG + chr7_1:73264459-73264478 Msa1036820:intron 25.0%
! TTAGGTATTTGGATCAATTT+GGG + chr7_1:73264460-73264479 Msa1036820:intron 25.0%
! CCTATCAATATATACACATA+TGG - chr7_1:73265378-73265397 None:intergenic 25.0%
! CCATATGTGTATATATTGAT+AGG + chr7_1:73265375-73265394 Msa1036820:three_prime_UTR 25.0%
AGGAAATAGGAAAGAAAAGT+TGG - chr7_1:73263300-73263319 None:intergenic 30.0%
!!! ACTATAGCTGTGGTTTTTTT+AGG - chr7_1:73263473-73263492 None:intergenic 30.0%
! AAAAACCACAGCTATAGTTT+TGG + chr7_1:73263475-73263494 Msa1036820:five_prime_UTR 30.0%
! AAAACCACAGCTATAGTTTT+GGG + chr7_1:73263476-73263495 Msa1036820:five_prime_UTR 30.0%
CACAAAATTCAAAACAACCT+TGG + chr7_1:73263752-73263771 Msa1036820:five_prime_UTR 30.0%
! GTTGTTATTCATGAAGTTAC+AGG - chr7_1:73263874-73263893 None:intergenic 30.0%
ACATATACCTTAGGAATTCA+AGG - chr7_1:73264115-73264134 None:intergenic 30.0%
ATGCAGTTGAAAAGAAAGAA+AGG - chr7_1:73264187-73264206 None:intergenic 30.0%
!! ATTTGACTTTTTAACCCACA+TGG + chr7_1:73264298-73264317 Msa1036820:intron 30.0%
TATCATGGTGTTTAGGTATT+TGG + chr7_1:73264449-73264468 Msa1036820:intron 30.0%
! ACACCAATTTGTCCATTTTA+TGG + chr7_1:73264498-73264517 Msa1036820:CDS 30.0%
! CACCAATTTGTCCATTTTAT+GGG + chr7_1:73264499-73264518 Msa1036820:CDS 30.0%
! AAACCAGAAACAAATCCTTT+TGG + chr7_1:73264627-73264646 Msa1036820:CDS 30.0%
TTGAAGCTTGTGAATATACA+TGG + chr7_1:73265037-73265056 Msa1036820:three_prime_UTR 30.0%
TCGATGTGGATAACATTATT+TGG + chr7_1:73265344-73265363 Msa1036820:three_prime_UTR 30.0%
AGGAAATAGGAAAGAAAAGT+TGG - chr7_1:73263300-73263319 None:intergenic 30.0%
!!! ACTATAGCTGTGGTTTTTTT+AGG - chr7_1:73263473-73263492 None:intergenic 30.0%
! AAAAACCACAGCTATAGTTT+TGG + chr7_1:73263475-73263494 Msa1036820:five_prime_UTR 30.0%
! AAAACCACAGCTATAGTTTT+GGG + chr7_1:73263476-73263495 Msa1036820:five_prime_UTR 30.0%
CACAAAATTCAAAACAACCT+TGG + chr7_1:73263752-73263771 Msa1036820:five_prime_UTR 30.0%
! GTTGTTATTCATGAAGTTAC+AGG - chr7_1:73263874-73263893 None:intergenic 30.0%
ACATATACCTTAGGAATTCA+AGG - chr7_1:73264115-73264134 None:intergenic 30.0%
ATGCAGTTGAAAAGAAAGAA+AGG - chr7_1:73264187-73264206 None:intergenic 30.0%
!! ATTTGACTTTTTAACCCACA+TGG + chr7_1:73264298-73264317 Msa1036820:intron 30.0%
TATCATGGTGTTTAGGTATT+TGG + chr7_1:73264449-73264468 Msa1036820:intron 30.0%
! ACACCAATTTGTCCATTTTA+TGG + chr7_1:73264498-73264517 Msa1036820:CDS 30.0%
! CACCAATTTGTCCATTTTAT+GGG + chr7_1:73264499-73264518 Msa1036820:CDS 30.0%
! AAACCAGAAACAAATCCTTT+TGG + chr7_1:73264627-73264646 Msa1036820:CDS 30.0%
TTGAAGCTTGTGAATATACA+TGG + chr7_1:73265037-73265056 Msa1036820:three_prime_UTR 30.0%
TCGATGTGGATAACATTATT+TGG + chr7_1:73265344-73265363 Msa1036820:three_prime_UTR 30.0%
!! TGTTAGGCTTTGAAGGAAAT+AGG - chr7_1:73263313-73263332 None:intergenic 35.0%
GAGAATGTGATGAGATTGAT+GGG - chr7_1:73263549-73263568 None:intergenic 35.0%
AGAGAATGTGATGAGATTGA+TGG - chr7_1:73263550-73263569 None:intergenic 35.0%
! CTTTCTGCTTCATCATTTTC+AGG + chr7_1:73263587-73263606 Msa1036820:five_prime_UTR 35.0%
!! AACACTTTCACTTTTGTCCA+AGG + chr7_1:73263674-73263693 Msa1036820:intron 35.0%
!! TGTTGTTGTTAAGCTTGTTG+TGG - chr7_1:73263922-73263941 None:intergenic 35.0%
ATTCTTGAGATACTGACCAA+AGG - chr7_1:73264048-73264067 None:intergenic 35.0%
AAAGAAAGGTTGCATTTGCA+TGG - chr7_1:73264173-73264192 None:intergenic 35.0%
TGATGCAATTGCAACTACTT+GGG - chr7_1:73264221-73264240 None:intergenic 35.0%
GAACGAGATTTAAACCATGT+GGG - chr7_1:73264315-73264334 None:intergenic 35.0%
TGAACGAGATTTAAACCATG+TGG - chr7_1:73264316-73264335 None:intergenic 35.0%
TGCCCATAAAATGGACAAAT+TGG - chr7_1:73264504-73264523 None:intergenic 35.0%
GATCTTCAATCAAAAGCAAG+AGG + chr7_1:73264687-73264706 Msa1036820:CDS 35.0%
! CATATAGAGTGCTTTGTCTT+TGG + chr7_1:73264887-73264906 Msa1036820:three_prime_UTR 35.0%
! TTCACAAGCTTCAACTTTTC+AGG - chr7_1:73265031-73265050 None:intergenic 35.0%
TCATAGCTGTTATTGTTGAG+TGG - chr7_1:73265099-73265118 None:intergenic 35.0%
!! GAACTTTGAAGCTACATTTG+TGG + chr7_1:73265295-73265314 Msa1036820:three_prime_UTR 35.0%
!! TGTTAGGCTTTGAAGGAAAT+AGG - chr7_1:73263313-73263332 None:intergenic 35.0%
GAGAATGTGATGAGATTGAT+GGG - chr7_1:73263549-73263568 None:intergenic 35.0%
AGAGAATGTGATGAGATTGA+TGG - chr7_1:73263550-73263569 None:intergenic 35.0%
! CTTTCTGCTTCATCATTTTC+AGG + chr7_1:73263587-73263606 Msa1036820:five_prime_UTR 35.0%
!! AACACTTTCACTTTTGTCCA+AGG + chr7_1:73263674-73263693 Msa1036820:intron 35.0%
!! TGTTGTTGTTAAGCTTGTTG+TGG - chr7_1:73263922-73263941 None:intergenic 35.0%
ATTCTTGAGATACTGACCAA+AGG - chr7_1:73264048-73264067 None:intergenic 35.0%
AAAGAAAGGTTGCATTTGCA+TGG - chr7_1:73264173-73264192 None:intergenic 35.0%
TGATGCAATTGCAACTACTT+GGG - chr7_1:73264221-73264240 None:intergenic 35.0%
GAACGAGATTTAAACCATGT+GGG - chr7_1:73264315-73264334 None:intergenic 35.0%
TGAACGAGATTTAAACCATG+TGG - chr7_1:73264316-73264335 None:intergenic 35.0%
TGCCCATAAAATGGACAAAT+TGG - chr7_1:73264504-73264523 None:intergenic 35.0%
GATCTTCAATCAAAAGCAAG+AGG + chr7_1:73264687-73264706 Msa1036820:CDS 35.0%
! CATATAGAGTGCTTTGTCTT+TGG + chr7_1:73264887-73264906 Msa1036820:three_prime_UTR 35.0%
! TTCACAAGCTTCAACTTTTC+AGG - chr7_1:73265031-73265050 None:intergenic 35.0%
TCATAGCTGTTATTGTTGAG+TGG - chr7_1:73265099-73265118 None:intergenic 35.0%
!! GAACTTTGAAGCTACATTTG+TGG + chr7_1:73265295-73265314 Msa1036820:three_prime_UTR 35.0%
GAAAAGTTGGACAGTAGGTT+TGG - chr7_1:73263287-73263306 None:intergenic 40.0%
GGAAAGAAAAGTTGGACAGT+AGG - chr7_1:73263292-73263311 None:intergenic 40.0%
! TCATCACTTGTTAGTGCTAG+TGG - chr7_1:73263396-73263415 None:intergenic 40.0%
!! GAGTTTGCTTCACCCTTTTA+AGG + chr7_1:73263435-73263454 Msa1036820:five_prime_UTR 40.0%
ACAACAACAACAACAGCTAG+TGG + chr7_1:73263933-73263952 Msa1036820:CDS 40.0%
CACATGTCCTTGAATTCCTA+AGG + chr7_1:73264105-73264124 Msa1036820:CDS 40.0%
GTGATGCAATTGCAACTACT+TGG - chr7_1:73264222-73264241 None:intergenic 40.0%
GGATTTGGATGCCCATAAAA+TGG - chr7_1:73264513-73264532 None:intergenic 40.0%
!!! CGTGCTGCTTTTGAAGAAAA+TGG + chr7_1:73264600-73264619 Msa1036820:CDS 40.0%
!!! GCTGCTTTTGAAGAAAATGG+AGG + chr7_1:73264603-73264622 Msa1036820:CDS 40.0%
! GCACCAAAAGGATTTGTTTC+TGG - chr7_1:73264633-73264652 None:intergenic 40.0%
TTCTGTCACAGCAAGTAAAC+AGG - chr7_1:73264915-73264934 None:intergenic 40.0%
TACTGGTAGTAGACTCATAG+TGG + chr7_1:73265005-73265024 Msa1036820:three_prime_UTR 40.0%
GCTGAGAAATGAGAATCACA+CGG - chr7_1:73265073-73265092 None:intergenic 40.0%
TCCATACATAGTGATCGATG+TGG + chr7_1:73265330-73265349 Msa1036820:three_prime_UTR 40.0%
TCCACATCGATCACTATGTA+TGG - chr7_1:73265334-73265353 None:intergenic 40.0%
GAAAAGTTGGACAGTAGGTT+TGG - chr7_1:73263287-73263306 None:intergenic 40.0%
GGAAAGAAAAGTTGGACAGT+AGG - chr7_1:73263292-73263311 None:intergenic 40.0%
! TCATCACTTGTTAGTGCTAG+TGG - chr7_1:73263396-73263415 None:intergenic 40.0%
!! GAGTTTGCTTCACCCTTTTA+AGG + chr7_1:73263435-73263454 Msa1036820:five_prime_UTR 40.0%
ACAACAACAACAACAGCTAG+TGG + chr7_1:73263933-73263952 Msa1036820:CDS 40.0%
CACATGTCCTTGAATTCCTA+AGG + chr7_1:73264105-73264124 Msa1036820:CDS 40.0%
GTGATGCAATTGCAACTACT+TGG - chr7_1:73264222-73264241 None:intergenic 40.0%
GGATTTGGATGCCCATAAAA+TGG - chr7_1:73264513-73264532 None:intergenic 40.0%
!!! CGTGCTGCTTTTGAAGAAAA+TGG + chr7_1:73264600-73264619 Msa1036820:CDS 40.0%
!!! GCTGCTTTTGAAGAAAATGG+AGG + chr7_1:73264603-73264622 Msa1036820:CDS 40.0%
! GCACCAAAAGGATTTGTTTC+TGG - chr7_1:73264633-73264652 None:intergenic 40.0%
TTCTGTCACAGCAAGTAAAC+AGG - chr7_1:73264915-73264934 None:intergenic 40.0%
TACTGGTAGTAGACTCATAG+TGG + chr7_1:73265005-73265024 Msa1036820:three_prime_UTR 40.0%
GCTGAGAAATGAGAATCACA+CGG - chr7_1:73265073-73265092 None:intergenic 40.0%
TCCATACATAGTGATCGATG+TGG + chr7_1:73265330-73265349 Msa1036820:three_prime_UTR 40.0%
TCCACATCGATCACTATGTA+TGG - chr7_1:73265334-73265353 None:intergenic 40.0%
AGTTGGACAGTAGGTTTGGA+TGG - chr7_1:73263283-73263302 None:intergenic 45.0%
!! AGAGAGGTGTTAGGCTTTGA+AGG - chr7_1:73263320-73263339 None:intergenic 45.0%
GCTTCCCAAAACTATAGCTG+TGG - chr7_1:73263483-73263502 None:intergenic 45.0%
!! CTTGTTCTTCTAGGGTTCCT+TGG - chr7_1:73263694-73263713 None:intergenic 45.0%
CAAGGAACCCTAGAAGAACA+AGG + chr7_1:73263692-73263711 Msa1036820:intron 45.0%
CTTTGTGCTTTGCAGATCCA+AGG - chr7_1:73263772-73263791 None:intergenic 45.0%
CTTGGATCTGCAAAGCACAA+AGG + chr7_1:73263770-73263789 Msa1036820:five_prime_UTR 45.0%
! ACTGTTGGGACTGTTGATGA+TGG - chr7_1:73263985-73264004 None:intergenic 45.0%
GGCGTTTCTGATTCTCATAG+CGG - chr7_1:73264013-73264032 None:intergenic 45.0%
GGTAGCTATGATGCGAATTG+TGG + chr7_1:73264392-73264411 Msa1036820:intron 45.0%
!! ATGTCCTCTAAGGCAAGCAT+GGG + chr7_1:73264548-73264567 Msa1036820:CDS 45.0%
!! ACTTGTTGTTGTTGTGGTGG+TGG - chr7_1:73264744-73264763 None:intergenic 45.0%
!! GGAACTTGTTGTTGTTGTGG+TGG - chr7_1:73264747-73264766 None:intergenic 45.0%
!! GGTGGAACTTGTTGTTGTTG+TGG - chr7_1:73264750-73264769 None:intergenic 45.0%
AGACAGTGCTGTGTTTGTAC+TGG + chr7_1:73264988-73265007 Msa1036820:three_prime_UTR 45.0%
AGTTGGACAGTAGGTTTGGA+TGG - chr7_1:73263283-73263302 None:intergenic 45.0%
!! AGAGAGGTGTTAGGCTTTGA+AGG - chr7_1:73263320-73263339 None:intergenic 45.0%
GCTTCCCAAAACTATAGCTG+TGG - chr7_1:73263483-73263502 None:intergenic 45.0%
!! CTTGTTCTTCTAGGGTTCCT+TGG - chr7_1:73263694-73263713 None:intergenic 45.0%
CAAGGAACCCTAGAAGAACA+AGG + chr7_1:73263692-73263711 Msa1036820:intron 45.0%
CTTTGTGCTTTGCAGATCCA+AGG - chr7_1:73263772-73263791 None:intergenic 45.0%
CTTGGATCTGCAAAGCACAA+AGG + chr7_1:73263770-73263789 Msa1036820:five_prime_UTR 45.0%
! ACTGTTGGGACTGTTGATGA+TGG - chr7_1:73263985-73264004 None:intergenic 45.0%
GGCGTTTCTGATTCTCATAG+CGG - chr7_1:73264013-73264032 None:intergenic 45.0%
GGTAGCTATGATGCGAATTG+TGG + chr7_1:73264392-73264411 Msa1036820:intron 45.0%
!! ATGTCCTCTAAGGCAAGCAT+GGG + chr7_1:73264548-73264567 Msa1036820:CDS 45.0%
!! ACTTGTTGTTGTTGTGGTGG+TGG - chr7_1:73264744-73264763 None:intergenic 45.0%
!! GGAACTTGTTGTTGTTGTGG+TGG - chr7_1:73264747-73264766 None:intergenic 45.0%
!! GGTGGAACTTGTTGTTGTTG+TGG - chr7_1:73264750-73264769 None:intergenic 45.0%
AGACAGTGCTGTGTTTGTAC+TGG + chr7_1:73264988-73265007 Msa1036820:three_prime_UTR 45.0%
!!! TATGATTTTTAATATTATTT+TGG + chr7_1:73264420-73264439 Msa1036820:intron 5.0%
!!! TATGATTTTTAATATTATTT+TGG + chr7_1:73264420-73264439 Msa1036820:intron 5.0%
CAGTAGGTTTGGATGGAGAC+AGG - chr7_1:73263276-73263295 None:intergenic 50.0%
!! GAGAGAGAGAGAGAGGTGTT+AGG - chr7_1:73263329-73263348 None:intergenic 50.0%
AGAGAGAGAGAGAGAGAGAG+AGG - chr7_1:73263334-73263353 None:intergenic 50.0%
GATGGTTGAAGCTGCAGATG+AGG - chr7_1:73263967-73263986 None:intergenic 50.0%
TCATAGCGGCTGCTACTGTT+GGG - chr7_1:73263999-73264018 None:intergenic 50.0%
AGAATCAGAAACGCCGTGAC+TGG + chr7_1:73264018-73264037 Msa1036820:CDS 50.0%
!! GACCAAAGGTGTTCCAGTCA+CGG - chr7_1:73264034-73264053 None:intergenic 50.0%
CACCATGTCCATGTCCTCTA+AGG + chr7_1:73264538-73264557 Msa1036820:CDS 50.0%
TGCCTTAGAGGACATGGACA+TGG - chr7_1:73264543-73264562 None:intergenic 50.0%
CATGCTTGCCTTAGAGGACA+TGG - chr7_1:73264549-73264568 None:intergenic 50.0%
!! CATGTCCTCTAAGGCAAGCA+TGG + chr7_1:73264547-73264566 Msa1036820:CDS 50.0%
!! TGTCCTCTAAGGCAAGCATG+GGG + chr7_1:73264549-73264568 Msa1036820:CDS 50.0%
GGAAGCCTTGATGCACTCAT+AGG + chr7_1:73264570-73264589 Msa1036820:CDS 50.0%
GACGACCTATGAGTGCATCA+AGG - chr7_1:73264578-73264597 None:intergenic 50.0%
GAAGATCACGAACTTCGCGA+AGG - chr7_1:73264674-73264693 None:intergenic 50.0%
CAGTAGGTTTGGATGGAGAC+AGG - chr7_1:73263276-73263295 None:intergenic 50.0%
!! GAGAGAGAGAGAGAGGTGTT+AGG - chr7_1:73263329-73263348 None:intergenic 50.0%
AGAGAGAGAGAGAGAGAGAG+AGG - chr7_1:73263334-73263353 None:intergenic 50.0%
GATGGTTGAAGCTGCAGATG+AGG - chr7_1:73263967-73263986 None:intergenic 50.0%
TCATAGCGGCTGCTACTGTT+GGG - chr7_1:73263999-73264018 None:intergenic 50.0%
AGAATCAGAAACGCCGTGAC+TGG + chr7_1:73264018-73264037 Msa1036820:CDS 50.0%
!! GACCAAAGGTGTTCCAGTCA+CGG - chr7_1:73264034-73264053 None:intergenic 50.0%
CACCATGTCCATGTCCTCTA+AGG + chr7_1:73264538-73264557 Msa1036820:CDS 50.0%
TGCCTTAGAGGACATGGACA+TGG - chr7_1:73264543-73264562 None:intergenic 50.0%
CATGCTTGCCTTAGAGGACA+TGG - chr7_1:73264549-73264568 None:intergenic 50.0%
!! CATGTCCTCTAAGGCAAGCA+TGG + chr7_1:73264547-73264566 Msa1036820:CDS 50.0%
!! TGTCCTCTAAGGCAAGCATG+GGG + chr7_1:73264549-73264568 Msa1036820:CDS 50.0%
GGAAGCCTTGATGCACTCAT+AGG + chr7_1:73264570-73264589 Msa1036820:CDS 50.0%
GACGACCTATGAGTGCATCA+AGG - chr7_1:73264578-73264597 None:intergenic 50.0%
GAAGATCACGAACTTCGCGA+AGG - chr7_1:73264674-73264693 None:intergenic 50.0%
CTCATAGCGGCTGCTACTGT+TGG - chr7_1:73264000-73264019 None:intergenic 55.0%
GTCCACCTCTTTCCCTCTCT+AGG + chr7_1:73264072-73264091 Msa1036820:CDS 55.0%
CACCTAGAGAGGGAAAGAGG+TGG - chr7_1:73264077-73264096 None:intergenic 55.0%
CTGCACCTAGAGAGGGAAAG+AGG - chr7_1:73264080-73264099 None:intergenic 55.0%
CTTTCCCTCTCTAGGTGCAG+CGG + chr7_1:73264080-73264099 Msa1036820:CDS 55.0%
!! GGACATGGTGCTGGTGGATT+TGG - chr7_1:73264528-73264547 None:intergenic 55.0%
AGAGGACATGGACATGGTGC+TGG - chr7_1:73264537-73264556 None:intergenic 55.0%
CTTCCCCATGCTTGCCTTAG+AGG - chr7_1:73264555-73264574 None:intergenic 55.0%
CTCACAGCTCGAGCACCAAA+AGG - chr7_1:73264645-73264664 None:intergenic 55.0%
!!! CTTTTGGTGCTCGAGCTGTG+AGG + chr7_1:73264643-73264662 Msa1036820:CDS 55.0%
CAAGTTCCACCACCAGCACA+AGG + chr7_1:73264759-73264778 Msa1036820:CDS 55.0%
GTTGCACTTGCACCTTGTGC+TGG - chr7_1:73264774-73264793 None:intergenic 55.0%
CTCATAGCGGCTGCTACTGT+TGG - chr7_1:73264000-73264019 None:intergenic 55.0%
GTCCACCTCTTTCCCTCTCT+AGG + chr7_1:73264072-73264091 Msa1036820:CDS 55.0%
CACCTAGAGAGGGAAAGAGG+TGG - chr7_1:73264077-73264096 None:intergenic 55.0%
CTGCACCTAGAGAGGGAAAG+AGG - chr7_1:73264080-73264099 None:intergenic 55.0%
CTTTCCCTCTCTAGGTGCAG+CGG + chr7_1:73264080-73264099 Msa1036820:CDS 55.0%
!! GGACATGGTGCTGGTGGATT+TGG - chr7_1:73264528-73264547 None:intergenic 55.0%
AGAGGACATGGACATGGTGC+TGG - chr7_1:73264537-73264556 None:intergenic 55.0%
CTTCCCCATGCTTGCCTTAG+AGG - chr7_1:73264555-73264574 None:intergenic 55.0%
CTCACAGCTCGAGCACCAAA+AGG - chr7_1:73264645-73264664 None:intergenic 55.0%
!!! CTTTTGGTGCTCGAGCTGTG+AGG + chr7_1:73264643-73264662 Msa1036820:CDS 55.0%
CAAGTTCCACCACCAGCACA+AGG + chr7_1:73264759-73264778 Msa1036820:CDS 55.0%
GTTGCACTTGCACCTTGTGC+TGG - chr7_1:73264774-73264793 None:intergenic 55.0%
CGCCGTGACTGGAACACCTT+TGG + chr7_1:73264029-73264048 Msa1036820:CDS 60.0%
TGCACCGCTGCACCTAGAGA+GGG - chr7_1:73264087-73264106 None:intergenic 60.0%
! GGACATGGACATGGTGCTGG+TGG - chr7_1:73264534-73264553 None:intergenic 60.0%
! CTTGCACCTTGTGCTGGTGG+TGG - chr7_1:73264768-73264787 None:intergenic 60.0%
GCACTTGCACCTTGTGCTGG+TGG - chr7_1:73264771-73264790 None:intergenic 60.0%
CGCCGTGACTGGAACACCTT+TGG + chr7_1:73264029-73264048 Msa1036820:CDS 60.0%
TGCACCGCTGCACCTAGAGA+GGG - chr7_1:73264087-73264106 None:intergenic 60.0%
! GGACATGGACATGGTGCTGG+TGG - chr7_1:73264534-73264553 None:intergenic 60.0%
! CTTGCACCTTGTGCTGGTGG+TGG - chr7_1:73264768-73264787 None:intergenic 60.0%
GCACTTGCACCTTGTGCTGG+TGG - chr7_1:73264771-73264790 None:intergenic 60.0%
GTGCACCGCTGCACCTAGAG+AGG - chr7_1:73264088-73264107 None:intergenic 65.0%
GTGCACCGCTGCACCTAGAG+AGG - chr7_1:73264088-73264107 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr7_1 gene 73263267 73265418 73263267 ID=Msa1036820;Name=Msa1036820
chr7_1 mRNA 73263267 73265418 73263267 ID=Msa1036820-mRNA-1;Parent=Msa1036820;Name=Msa1036820-mRNA-1;_AED=0.03;_eAED=0.03;_QI=468|1|1|1|0.5|0.33|3|618|213
chr7_1 exon 73263267 73263608 73263267 ID=Msa1036820-mRNA-1:exon:17967;Parent=Msa1036820-mRNA-1
chr7_1 exon 73263696 73264126 73263696 ID=Msa1036820-mRNA-1:exon:17968;Parent=Msa1036820-mRNA-1
chr7_1 exon 73264464 73265418 73264464 ID=Msa1036820-mRNA-1:exon:17969;Parent=Msa1036820-mRNA-1
chr7_1 five_prime_UTR 73263267 73263608 73263267 ID=Msa1036820-mRNA-1:five_prime_utr;Parent=Msa1036820-mRNA-1
chr7_1 five_prime_UTR 73263696 73263821 73263696 ID=Msa1036820-mRNA-1:five_prime_utr;Parent=Msa1036820-mRNA-1
chr7_1 CDS 73263822 73264126 73263822 ID=Msa1036820-mRNA-1:cds;Parent=Msa1036820-mRNA-1
chr7_1 CDS 73264464 73264800 73264464 ID=Msa1036820-mRNA-1:cds;Parent=Msa1036820-mRNA-1
chr7_1 three_prime_UTR 73264801 73265418 73264801 ID=Msa1036820-mRNA-1:three_prime_utr;Parent=Msa1036820-mRNA-1
Gene Sequence

>Msa1036820

ATGGATTCAATTCAAGAATTTATAGGGACATGTAACAATGAAAACATGACCTGTAACTTCATGAATAACAACAACAACAACAACAGTATAAGTACAACCACAACAAGCTTAACAACAACAACAACAGCTAGTGGAAGCTCTTCCTCATCTGCAGCTTCAACCATCATCAACAGTCCCAACAGTAGCAGCCGCTATGAGAATCAGAAACGCCGTGACTGGAACACCTTTGGTCAGTATCTCAAGAATCATCGTCCACCTCTTTCCCTCTCTAGGTGCAGCGGTGCACATGTCCTTGAATTCCTAAGGTATTTGGATCAATTTGGGAAGACAAAAGTTCACACACCAATTTGTCCATTTTATGGGCATCCAAATCCACCAGCACCATGTCCATGTCCTCTAAGGCAAGCATGGGGAAGCCTTGATGCACTCATAGGTCGTCTTCGTGCTGCTTTTGAAGAAAATGGAGGAAAACCAGAAACAAATCCTTTTGGTGCTCGAGCTGTGAGGCTCTACCTTCGCGAAGTTCGTGATCTTCAATCAAAAGCAAGAGGTATTAGTTACGAGAAGAAGAAAAGAAAGCGTCCACCACCACAACAACAACAAGTTCCACCACCAGCACAAGGTGCAAGTGCAACTCATTAG
Protein sequence

>Msa1036820

MDSIQEFIGTCNNENMTCNFMNNNNNNNSISTTTTSLTTTTTASGSSSSSAASTIINSPNSSSRYENQKRRDWNTFGQYLKNHRPPLSLSRCSGAHVLEFLRYLDQFGKTKVHTPICPFYGHPNPPAPCPCPLRQAWGSLDALIGRLRAAFEENGGKPETNPFGARAVRLYLREVRDLQSKARGISYEKKKRKRPPPQQQQVPPPAQGASATH*