AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1163920

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1163920 MtrunA17_Chr7g0261381 98.131 214 2 2 1 212 1 214 4.92e-156 430
Msa1163920 MtrunA17_Chr1g0167411 73.575 193 41 2 1 187 1 189 9.37e-94 273
Msa1163920 MtrunA17_Chr1g0183761 100.000 131 0 0 63 193 45 175 3.89e-87 256
Msa1163920 MtrunA17_Chr1g0187671 81.325 166 31 0 28 193 18 183 2.35e-85 251
Msa1163920 MtrunA17_Chr4g0051251 82.609 138 24 0 62 199 45 182 2.95e-85 252
Msa1163920 MtrunA17_Chr5g0431211 80.451 133 26 0 62 194 36 168 1.34e-82 245
Msa1163920 MtrunA17_Chr3g0105921 81.818 132 24 0 63 194 42 173 1.12e-73 222
Msa1163920 MtrunA17_Chr2g0325831 71.724 145 34 1 42 186 9 146 3.93e-73 219
Msa1163920 MtrunA17_Chr7g0274631 65.432 162 48 3 34 194 4 158 4.71e-72 216
Msa1163920 MtrunA17_Chr4g0041141 65.333 150 52 0 37 186 3 152 4.52e-71 214
Msa1163920 MtrunA17_Chr2g0283861 75.758 132 32 0 62 193 34 165 9.82e-71 213
Msa1163920 MtrunA17_Chr2g0315791 75.000 132 33 0 62 193 32 163 4.69e-70 211
Msa1163920 MtrunA17_Chr1g0190231 71.250 160 46 0 28 187 5 164 1.74e-69 210
Msa1163920 MtrunA17_Chr8g0379061 72.800 125 34 0 62 186 84 208 2.97e-55 176
Msa1163920 MtrunA17_Chr1g0211641 50.485 103 24 2 26 101 7 109 1.95e-23 90.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1163920 AT2G31160.1 80.814 172 30 1 25 196 19 187 4.17e-88 259
Msa1163920 AT1G07090.1 76.389 144 34 0 52 195 20 163 2.65e-84 248
Msa1163920 AT3G23290.2 73.262 187 35 2 1 187 1 172 1.63e-80 239
Msa1163920 AT5G58500.1 79.104 134 27 1 62 194 18 151 7.21e-79 234
Msa1163920 AT2G42610.1 75.714 140 34 0 58 197 20 159 6.97e-74 221
Msa1163920 AT2G42610.2 75.714 140 34 0 58 197 20 159 6.97e-74 221
Msa1163920 AT5G28490.1 83.088 136 23 0 57 192 19 154 1.31e-72 218
Msa1163920 AT3G04510.1 80.000 130 26 0 63 192 33 162 1.49e-69 211
Msa1163920 AT1G78815.1 67.200 125 41 0 62 186 39 163 1.17e-62 193
Msa1163920 AT4G18610.1 69.048 126 38 1 62 186 36 161 4.86e-62 192
Msa1163920 AT1G16910.1 61.194 134 52 0 58 191 18 151 3.06e-58 181

Find 78 sgRNAs with CRISPR-Local

Find 231 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATCATGGTGTTTAGGTATT+TGG 0.100645 7_4:+55383063 Msa1163920:intron
TTTAGGTATTTGGATCAATT+TGG 0.178063 7_4:+55383073 Msa1163920:intron
GCACCAAAAGGATTTGTTTC+TGG 0.187984 7_4:-55383244 None:intergenic
AATTAATACCTTGTTCTTCT+AGG 0.210004 7_4:-55382314 None:intergenic
CATATAGAGTGCTTTGTCTT+TGG 0.231876 7_4:+55383501 Msa1163920:three_prime_UTR
CTTCCCCATGCTTGCCTTAG+AGG 0.241570 7_4:-55383166 None:intergenic
GATTCAATTCAAGAATTTAT+AGG 0.256232 7_4:+55382439 Msa1163920:CDS
GAATTGAATCCATGAAAATA+TGG 0.280646 7_4:-55382426 None:intergenic
AAGAACAAGGTATTAATTCT+TGG 0.280943 7_4:+55382319 Msa1163920:five_prime_UTR
GTTGTTATTCATGAAGTTAC+AGG 0.282953 7_4:-55382485 None:intergenic
GGACATGGTGCTGGTGGATT+TGG 0.312240 7_4:-55383139 None:intergenic
ATTCAATTCAAGAATTTATA+GGG 0.321871 7_4:+55382440 Msa1163920:CDS
TGTTAGGCTTTGAAGGAAAT+AGG 0.324854 7_4:-55381913 None:intergenic
AGAGAGGTGTTAGGCTTTGA+AGG 0.331061 7_4:-55381920 None:intergenic
GGATTTGGATGCCCATAAAA+TGG 0.348197 7_4:-55383124 None:intergenic
TTAGGTATTTGGATCAATTT+GGG 0.353257 7_4:+55383074 Msa1163920:intron
CACCATGTCCATGTCCTCTA+AGG 0.357440 7_4:+55383152 Msa1163920:CDS
CGCCGTGACTGGAACACCTT+TGG 0.383976 7_4:+55382640 Msa1163920:CDS
ATATTAATATTCTTCATTCA+TGG 0.385287 7_4:-55381983 None:intergenic
TGCCCATAAAATGGACAAAT+TGG 0.388714 7_4:-55383115 None:intergenic
GAAAAGTTGGACAGTAGGTT+TGG 0.403335 7_4:-55381887 None:intergenic
CTCATAGCGGCTGCTACTGT+TGG 0.404554 7_4:-55382608 None:intergenic
AGGAAATAGGAAAGAAAAGT+TGG 0.404681 7_4:-55381900 None:intergenic
TTCTGTCACAGCAAGTAAAC+AGG 0.405995 7_4:-55383526 None:intergenic
AAACCAGAAACAAATCCTTT+TGG 0.416713 7_4:+55383241 Msa1163920:CDS
ATTAATACCTTGTTCTTCTA+GGG 0.448154 7_4:-55382313 None:intergenic
AGAATCAGAAACGCCGTGAC+TGG 0.455928 7_4:+55382629 Msa1163920:CDS
GTTGCACTTGCACCTTGTGC+TGG 0.465381 7_4:-55383385 None:intergenic
ACTGTTGGGACTGTTGATGA+TGG 0.472335 7_4:-55382593 None:intergenic
CTTGCACCTTGTGCTGGTGG+TGG 0.478161 7_4:-55383379 None:intergenic
ACATATACCTTAGGAATTCA+AGG 0.481244 7_4:-55382723 None:intergenic
GGAAAGAAAAGTTGGACAGT+AGG 0.482092 7_4:-55381892 None:intergenic
CACAAAATTCAAAACAACCT+TGG 0.482839 7_4:+55382366 Msa1163920:five_prime_UTR
AGTTGGACAGTAGGTTTGGA+TGG 0.483212 7_4:-55381883 None:intergenic
AGAGAGAGAAAGAGAGAGAG+AGG 0.484976 7_4:-55381936 None:intergenic
TCATAGCGGCTGCTACTGTT+GGG 0.492824 7_4:-55382607 None:intergenic
GGTGGAACTTGTTGTTGTTG+TGG 0.502983 7_4:-55383361 None:intergenic
TCATCACTTGTTAGTGCTAG+TGG 0.505405 7_4:-55382008 None:intergenic
CTTTGTGCTTTGCAGATCCA+AGG 0.509553 7_4:-55382383 None:intergenic
ACTTGTTGTTGTTGTGGTGG+TGG 0.511066 7_4:-55383355 None:intergenic
CTCACAGCTCGAGCACCAAA+AGG 0.515193 7_4:-55383256 None:intergenic
CACATGTCCTTGAATTCCTA+AGG 0.521843 7_4:+55382716 Msa1163920:CDS
GGAAGCCTTGATGCACTCAT+AGG 0.523874 7_4:+55383184 Msa1163920:CDS
CATGTCCTCTAAGGCAAGCA+TGG 0.527761 7_4:+55383161 Msa1163920:CDS
CAGTAGGTTTGGATGGAGAC+AGG 0.529323 7_4:-55381876 None:intergenic
CATGCTTGCCTTAGAGGACA+TGG 0.533497 7_4:-55383160 None:intergenic
GGAACTTGTTGTTGTTGTGG+TGG 0.534959 7_4:-55383358 None:intergenic
GCACTTGCACCTTGTGCTGG+TGG 0.535986 7_4:-55383382 None:intergenic
AGAGAATGTGATGAGATTGA+TGG 0.544970 7_4:-55382162 None:intergenic
GTCCACCTCTTTCCCTCTCT+AGG 0.545320 7_4:+55382683 Msa1163920:CDS
AGAGGACATGGACATGGTGC+TGG 0.548410 7_4:-55383148 None:intergenic
ATGTCCTCTAAGGCAAGCAT+GGG 0.548889 7_4:+55383162 Msa1163920:CDS
TTGAAGCTTGTGAATATACA+TGG 0.551801 7_4:+55383651 Msa1163920:three_prime_UTR
TGTTGTTGTTAAGCTTGTTG+TGG 0.560960 7_4:-55382530 None:intergenic
GACCAAAGGTGTTCCAGTCA+CGG 0.570044 7_4:-55382642 None:intergenic
TACTGGTAGTAGACTCATAG+TGG 0.572208 7_4:+55383619 Msa1163920:three_prime_UTR
AGACAGTGCTGTGTTTGTAC+TGG 0.573730 7_4:+55383602 Msa1163920:three_prime_UTR
CTGCACCTAGAGAGGGAAAG+AGG 0.576931 7_4:-55382688 None:intergenic
GATCTTCAATCAAAAGCAAG+AGG 0.593770 7_4:+55383301 Msa1163920:CDS
GCTTCCCAAAACTATAGCTG+TGG 0.599047 7_4:-55382095 None:intergenic
TCATAGCTGTTATTGTTGAG+TGG 0.610117 7_4:-55383710 None:intergenic
GAGAATGTGATGAGATTGAT+GGG 0.614654 7_4:-55382161 None:intergenic
GTGCACCGCTGCACCTAGAG+AGG 0.622701 7_4:-55382696 None:intergenic
CTTTCCCTCTCTAGGTGCAG+CGG 0.626380 7_4:+55382691 Msa1163920:CDS
CAAGGAACCCTAGAAGAACA+AGG 0.629527 7_4:+55382306 Msa1163920:intron
GACGACCTATGAGTGCATCA+AGG 0.630031 7_4:-55383189 None:intergenic
CAAGTTCCACCACCAGCACA+AGG 0.630971 7_4:+55383373 Msa1163920:CDS
GATGGTTGAAGCTGCAGATG+AGG 0.631628 7_4:-55382575 None:intergenic
TGCCTTAGAGGACATGGACA+TGG 0.634202 7_4:-55383154 None:intergenic
CTTGGATCTGCAAAGCACAA+AGG 0.637595 7_4:+55382384 Msa1163920:five_prime_UTR
TGCACCGCTGCACCTAGAGA+GGG 0.638717 7_4:-55382695 None:intergenic
GGACATGGACATGGTGCTGG+TGG 0.656231 7_4:-55383145 None:intergenic
GAAGATCACGAACTTCGCGA+AGG 0.664694 7_4:-55383285 None:intergenic
GGCGTTTCTGATTCTCATAG+CGG 0.670856 7_4:-55382621 None:intergenic
ATTCTTGAGATACTGACCAA+AGG 0.683345 7_4:-55382656 None:intergenic
CACCTAGAGAGGGAAAGAGG+TGG 0.686727 7_4:-55382685 None:intergenic
TGTCCTCTAAGGCAAGCATG+GGG 0.718093 7_4:+55383163 Msa1163920:CDS
GCTGAGAAATGAGAATCACA+CGG 0.732864 7_4:-55383684 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ACTATTTTTATGTTTATTTT+AGG - chr7_4:55382249-55382268 None:intergenic 10.0%
!!! ACTTTTTTTTAATCATATTT+AGG + chr7_4:55383445-55383464 Msa1163920:three_prime_UTR 10.0%
!!! CTTTTTTTTAATCATATTTA+GGG + chr7_4:55383446-55383465 Msa1163920:three_prime_UTR 10.0%
!!! ACTATTTTTATGTTTATTTT+AGG - chr7_4:55382249-55382268 None:intergenic 10.0%
!!! ACTTTTTTTTAATCATATTT+AGG + chr7_4:55383445-55383464 Msa1163920:three_prime_UTR 10.0%
!!! CTTTTTTTTAATCATATTTA+GGG + chr7_4:55383446-55383465 Msa1163920:three_prime_UTR 10.0%
!! ATATTAATATTCTTCATTCA+TGG - chr7_4:55381986-55382005 None:intergenic 15.0%
!!! TAGTTTTTATTTCCTTAAAA+GGG - chr7_4:55382065-55382084 None:intergenic 15.0%
!!! TTTATGTTTATTTTAGGAAT+AGG - chr7_4:55382243-55382262 None:intergenic 15.0%
!! ATTCAATTCAAGAATTTATA+GGG + chr7_4:55382440-55382459 Msa1163920:CDS 15.0%
!!! TTATTTTGGTTAATTTATCA+TGG + chr7_4:55383048-55383067 Msa1163920:intron 15.0%
!! ATATTAATATTCTTCATTCA+TGG - chr7_4:55381986-55382005 None:intergenic 15.0%
!!! TAGTTTTTATTTCCTTAAAA+GGG - chr7_4:55382065-55382084 None:intergenic 15.0%
!!! TTTATGTTTATTTTAGGAAT+AGG - chr7_4:55382243-55382262 None:intergenic 15.0%
!! ATTCAATTCAAGAATTTATA+GGG + chr7_4:55382440-55382459 Msa1163920:CDS 15.0%
!!! TTATTTTGGTTAATTTATCA+TGG + chr7_4:55383048-55383067 Msa1163920:intron 15.0%
!!! GTAGTTTTTATTTCCTTAAA+AGG - chr7_4:55382066-55382085 None:intergenic 20.0%
!!! AAGTGAAAGTGTTAATTAAT+TGG - chr7_4:55382282-55382301 None:intergenic 20.0%
!! GATTCAATTCAAGAATTTAT+AGG + chr7_4:55382439-55382458 Msa1163920:CDS 20.0%
!!! TGCATCACAATTTTTTATAT+CGG + chr7_4:55382848-55382867 Msa1163920:intron 20.0%
!!! GTAGTTTTTATTTCCTTAAA+AGG - chr7_4:55382066-55382085 None:intergenic 20.0%
!!! AAGTGAAAGTGTTAATTAAT+TGG - chr7_4:55382282-55382301 None:intergenic 20.0%
!! GATTCAATTCAAGAATTTAT+AGG + chr7_4:55382439-55382458 Msa1163920:CDS 20.0%
!!! TGCATCACAATTTTTTATAT+CGG + chr7_4:55382848-55382867 Msa1163920:intron 20.0%
! ATTAATACCTTGTTCTTCTA+GGG - chr7_4:55382316-55382335 None:intergenic 25.0%
! AATTAATACCTTGTTCTTCT+AGG - chr7_4:55382317-55382336 None:intergenic 25.0%
!! AAGAACAAGGTATTAATTCT+TGG + chr7_4:55382319-55382338 Msa1163920:five_prime_UTR 25.0%
!! AAAGTTAAGCCATATTTTCA+TGG + chr7_4:55382417-55382436 Msa1163920:exon 25.0%
! GAATTGAATCCATGAAAATA+TGG - chr7_4:55382429-55382448 None:intergenic 25.0%
! ATTTGTACTACATATACCTT+AGG - chr7_4:55382735-55382754 None:intergenic 25.0%
! GTCATTATATAACTCAATTG+CGG + chr7_4:55382985-55383004 Msa1163920:intron 25.0%
! GTTAATTTATCATGGTGTTT+AGG + chr7_4:55383056-55383075 Msa1163920:intron 25.0%
! TTTAGGTATTTGGATCAATT+TGG + chr7_4:55383073-55383092 Msa1163920:intron 25.0%
! TTAGGTATTTGGATCAATTT+GGG + chr7_4:55383074-55383093 Msa1163920:intron 25.0%
! ATTAATACCTTGTTCTTCTA+GGG - chr7_4:55382316-55382335 None:intergenic 25.0%
! AATTAATACCTTGTTCTTCT+AGG - chr7_4:55382317-55382336 None:intergenic 25.0%
!! AAGAACAAGGTATTAATTCT+TGG + chr7_4:55382319-55382338 Msa1163920:five_prime_UTR 25.0%
!! AAAGTTAAGCCATATTTTCA+TGG + chr7_4:55382417-55382436 Msa1163920:exon 25.0%
! GAATTGAATCCATGAAAATA+TGG - chr7_4:55382429-55382448 None:intergenic 25.0%
! ATTTGTACTACATATACCTT+AGG - chr7_4:55382735-55382754 None:intergenic 25.0%
! GTCATTATATAACTCAATTG+CGG + chr7_4:55382985-55383004 Msa1163920:intron 25.0%
! GTTAATTTATCATGGTGTTT+AGG + chr7_4:55383056-55383075 Msa1163920:intron 25.0%
! TTTAGGTATTTGGATCAATT+TGG + chr7_4:55383073-55383092 Msa1163920:intron 25.0%
! TTAGGTATTTGGATCAATTT+GGG + chr7_4:55383074-55383093 Msa1163920:intron 25.0%
AGGAAATAGGAAAGAAAAGT+TGG - chr7_4:55381903-55381922 None:intergenic 30.0%
!!! ACTATAGCTGTGGTTTTTTT+AGG - chr7_4:55382088-55382107 None:intergenic 30.0%
! AAAAACCACAGCTATAGTTT+TGG + chr7_4:55382090-55382109 Msa1163920:five_prime_UTR 30.0%
! AAAACCACAGCTATAGTTTT+GGG + chr7_4:55382091-55382110 Msa1163920:five_prime_UTR 30.0%
CACAAAATTCAAAACAACCT+TGG + chr7_4:55382366-55382385 Msa1163920:five_prime_UTR 30.0%
! GTTGTTATTCATGAAGTTAC+AGG - chr7_4:55382488-55382507 None:intergenic 30.0%
ACATATACCTTAGGAATTCA+AGG - chr7_4:55382726-55382745 None:intergenic 30.0%
ATGCAGTTGAAAAGAAAGAA+AGG - chr7_4:55382801-55382820 None:intergenic 30.0%
!! ATTTGACTTTTTAACCCACA+TGG + chr7_4:55382912-55382931 Msa1163920:intron 30.0%
TATCATGGTGTTTAGGTATT+TGG + chr7_4:55383063-55383082 Msa1163920:intron 30.0%
! ACACCAATTTGTCCATTTTA+TGG + chr7_4:55383112-55383131 Msa1163920:CDS 30.0%
! CACCAATTTGTCCATTTTAT+GGG + chr7_4:55383113-55383132 Msa1163920:CDS 30.0%
! AAACCAGAAACAAATCCTTT+TGG + chr7_4:55383241-55383260 Msa1163920:CDS 30.0%
TTGAAGCTTGTGAATATACA+TGG + chr7_4:55383651-55383670 Msa1163920:three_prime_UTR 30.0%
AGGAAATAGGAAAGAAAAGT+TGG - chr7_4:55381903-55381922 None:intergenic 30.0%
!!! ACTATAGCTGTGGTTTTTTT+AGG - chr7_4:55382088-55382107 None:intergenic 30.0%
! AAAAACCACAGCTATAGTTT+TGG + chr7_4:55382090-55382109 Msa1163920:five_prime_UTR 30.0%
! AAAACCACAGCTATAGTTTT+GGG + chr7_4:55382091-55382110 Msa1163920:five_prime_UTR 30.0%
CACAAAATTCAAAACAACCT+TGG + chr7_4:55382366-55382385 Msa1163920:five_prime_UTR 30.0%
! GTTGTTATTCATGAAGTTAC+AGG - chr7_4:55382488-55382507 None:intergenic 30.0%
ACATATACCTTAGGAATTCA+AGG - chr7_4:55382726-55382745 None:intergenic 30.0%
ATGCAGTTGAAAAGAAAGAA+AGG - chr7_4:55382801-55382820 None:intergenic 30.0%
!! ATTTGACTTTTTAACCCACA+TGG + chr7_4:55382912-55382931 Msa1163920:intron 30.0%
TATCATGGTGTTTAGGTATT+TGG + chr7_4:55383063-55383082 Msa1163920:intron 30.0%
! ACACCAATTTGTCCATTTTA+TGG + chr7_4:55383112-55383131 Msa1163920:CDS 30.0%
! CACCAATTTGTCCATTTTAT+GGG + chr7_4:55383113-55383132 Msa1163920:CDS 30.0%
! AAACCAGAAACAAATCCTTT+TGG + chr7_4:55383241-55383260 Msa1163920:CDS 30.0%
TTGAAGCTTGTGAATATACA+TGG + chr7_4:55383651-55383670 Msa1163920:three_prime_UTR 30.0%
!! TGTTAGGCTTTGAAGGAAAT+AGG - chr7_4:55381916-55381935 None:intergenic 35.0%
GAGAATGTGATGAGATTGAT+GGG - chr7_4:55382164-55382183 None:intergenic 35.0%
AGAGAATGTGATGAGATTGA+TGG - chr7_4:55382165-55382184 None:intergenic 35.0%
! CTTTCTGCTTCATCATTTTC+AGG + chr7_4:55382202-55382221 Msa1163920:five_prime_UTR 35.0%
TAACACTTTCACTTTGTCCA+AGG + chr7_4:55382288-55382307 Msa1163920:intron 35.0%
!! TGTTGTTGTTAAGCTTGTTG+TGG - chr7_4:55382533-55382552 None:intergenic 35.0%
ATTCTTGAGATACTGACCAA+AGG - chr7_4:55382659-55382678 None:intergenic 35.0%
AAAGAAAGGTTGCATTTGCA+TGG - chr7_4:55382787-55382806 None:intergenic 35.0%
TGATGCAATTGCAACTACTT+GGG - chr7_4:55382835-55382854 None:intergenic 35.0%
GAACGAGATTTAAACCATGT+GGG - chr7_4:55382929-55382948 None:intergenic 35.0%
TGAACGAGATTTAAACCATG+TGG - chr7_4:55382930-55382949 None:intergenic 35.0%
TGCCCATAAAATGGACAAAT+TGG - chr7_4:55383118-55383137 None:intergenic 35.0%
GATCTTCAATCAAAAGCAAG+AGG + chr7_4:55383301-55383320 Msa1163920:CDS 35.0%
! CATATAGAGTGCTTTGTCTT+TGG + chr7_4:55383501-55383520 Msa1163920:three_prime_UTR 35.0%
! TTCACAAGCTTCAACTTTTC+AGG - chr7_4:55383645-55383664 None:intergenic 35.0%
TCATAGCTGTTATTGTTGAG+TGG - chr7_4:55383713-55383732 None:intergenic 35.0%
!! TGTTAGGCTTTGAAGGAAAT+AGG - chr7_4:55381916-55381935 None:intergenic 35.0%
GAGAATGTGATGAGATTGAT+GGG - chr7_4:55382164-55382183 None:intergenic 35.0%
AGAGAATGTGATGAGATTGA+TGG - chr7_4:55382165-55382184 None:intergenic 35.0%
! CTTTCTGCTTCATCATTTTC+AGG + chr7_4:55382202-55382221 Msa1163920:five_prime_UTR 35.0%
TAACACTTTCACTTTGTCCA+AGG + chr7_4:55382288-55382307 Msa1163920:intron 35.0%
!! TGTTGTTGTTAAGCTTGTTG+TGG - chr7_4:55382533-55382552 None:intergenic 35.0%
ATTCTTGAGATACTGACCAA+AGG - chr7_4:55382659-55382678 None:intergenic 35.0%
AAAGAAAGGTTGCATTTGCA+TGG - chr7_4:55382787-55382806 None:intergenic 35.0%
TGATGCAATTGCAACTACTT+GGG - chr7_4:55382835-55382854 None:intergenic 35.0%
GAACGAGATTTAAACCATGT+GGG - chr7_4:55382929-55382948 None:intergenic 35.0%
TGAACGAGATTTAAACCATG+TGG - chr7_4:55382930-55382949 None:intergenic 35.0%
TGCCCATAAAATGGACAAAT+TGG - chr7_4:55383118-55383137 None:intergenic 35.0%
GATCTTCAATCAAAAGCAAG+AGG + chr7_4:55383301-55383320 Msa1163920:CDS 35.0%
! CATATAGAGTGCTTTGTCTT+TGG + chr7_4:55383501-55383520 Msa1163920:three_prime_UTR 35.0%
! TTCACAAGCTTCAACTTTTC+AGG - chr7_4:55383645-55383664 None:intergenic 35.0%
TCATAGCTGTTATTGTTGAG+TGG - chr7_4:55383713-55383732 None:intergenic 35.0%
GAAAAGTTGGACAGTAGGTT+TGG - chr7_4:55381890-55381909 None:intergenic 40.0%
GGAAAGAAAAGTTGGACAGT+AGG - chr7_4:55381895-55381914 None:intergenic 40.0%
! TCATCACTTGTTAGTGCTAG+TGG - chr7_4:55382011-55382030 None:intergenic 40.0%
!! GAGTTTGCTTCACCCTTTTA+AGG + chr7_4:55382050-55382069 Msa1163920:five_prime_UTR 40.0%
ACAACAACAACAACAGCTAG+TGG + chr7_4:55382544-55382563 Msa1163920:CDS 40.0%
CACATGTCCTTGAATTCCTA+AGG + chr7_4:55382716-55382735 Msa1163920:CDS 40.0%
GTGATGCAATTGCAACTACT+TGG - chr7_4:55382836-55382855 None:intergenic 40.0%
GGATTTGGATGCCCATAAAA+TGG - chr7_4:55383127-55383146 None:intergenic 40.0%
!!! CGTGCTGCTTTTGAAGAAAA+TGG + chr7_4:55383214-55383233 Msa1163920:CDS 40.0%
!!! GCTGCTTTTGAAGAAAATGG+AGG + chr7_4:55383217-55383236 Msa1163920:CDS 40.0%
! GCACCAAAAGGATTTGTTTC+TGG - chr7_4:55383247-55383266 None:intergenic 40.0%
TTCTGTCACAGCAAGTAAAC+AGG - chr7_4:55383529-55383548 None:intergenic 40.0%
TACTGGTAGTAGACTCATAG+TGG + chr7_4:55383619-55383638 Msa1163920:three_prime_UTR 40.0%
GCTGAGAAATGAGAATCACA+CGG - chr7_4:55383687-55383706 None:intergenic 40.0%
GAAAAGTTGGACAGTAGGTT+TGG - chr7_4:55381890-55381909 None:intergenic 40.0%
GGAAAGAAAAGTTGGACAGT+AGG - chr7_4:55381895-55381914 None:intergenic 40.0%
! TCATCACTTGTTAGTGCTAG+TGG - chr7_4:55382011-55382030 None:intergenic 40.0%
!! GAGTTTGCTTCACCCTTTTA+AGG + chr7_4:55382050-55382069 Msa1163920:five_prime_UTR 40.0%
ACAACAACAACAACAGCTAG+TGG + chr7_4:55382544-55382563 Msa1163920:CDS 40.0%
CACATGTCCTTGAATTCCTA+AGG + chr7_4:55382716-55382735 Msa1163920:CDS 40.0%
GTGATGCAATTGCAACTACT+TGG - chr7_4:55382836-55382855 None:intergenic 40.0%
GGATTTGGATGCCCATAAAA+TGG - chr7_4:55383127-55383146 None:intergenic 40.0%
!!! CGTGCTGCTTTTGAAGAAAA+TGG + chr7_4:55383214-55383233 Msa1163920:CDS 40.0%
!!! GCTGCTTTTGAAGAAAATGG+AGG + chr7_4:55383217-55383236 Msa1163920:CDS 40.0%
! GCACCAAAAGGATTTGTTTC+TGG - chr7_4:55383247-55383266 None:intergenic 40.0%
TTCTGTCACAGCAAGTAAAC+AGG - chr7_4:55383529-55383548 None:intergenic 40.0%
TACTGGTAGTAGACTCATAG+TGG + chr7_4:55383619-55383638 Msa1163920:three_prime_UTR 40.0%
GCTGAGAAATGAGAATCACA+CGG - chr7_4:55383687-55383706 None:intergenic 40.0%
AGTTGGACAGTAGGTTTGGA+TGG - chr7_4:55381886-55381905 None:intergenic 45.0%
!! AGAGAGGTGTTAGGCTTTGA+AGG - chr7_4:55381923-55381942 None:intergenic 45.0%
!! GAAAGAGAGAGAGAGGTGTT+AGG - chr7_4:55381932-55381951 None:intergenic 45.0%
AGAGAGAGAAAGAGAGAGAG+AGG - chr7_4:55381939-55381958 None:intergenic 45.0%
GCTTCCCAAAACTATAGCTG+TGG - chr7_4:55382098-55382117 None:intergenic 45.0%
!! CTTGTTCTTCTAGGGTTCCT+TGG - chr7_4:55382308-55382327 None:intergenic 45.0%
CAAGGAACCCTAGAAGAACA+AGG + chr7_4:55382306-55382325 Msa1163920:intron 45.0%
CTTTGTGCTTTGCAGATCCA+AGG - chr7_4:55382386-55382405 None:intergenic 45.0%
CTTGGATCTGCAAAGCACAA+AGG + chr7_4:55382384-55382403 Msa1163920:five_prime_UTR 45.0%
! ACTGTTGGGACTGTTGATGA+TGG - chr7_4:55382596-55382615 None:intergenic 45.0%
GGCGTTTCTGATTCTCATAG+CGG - chr7_4:55382624-55382643 None:intergenic 45.0%
GGTAGCTATGATGCGAATTG+TGG + chr7_4:55383006-55383025 Msa1163920:intron 45.0%
!! ATGTCCTCTAAGGCAAGCAT+GGG + chr7_4:55383162-55383181 Msa1163920:CDS 45.0%
!! ACTTGTTGTTGTTGTGGTGG+TGG - chr7_4:55383358-55383377 None:intergenic 45.0%
!! GGAACTTGTTGTTGTTGTGG+TGG - chr7_4:55383361-55383380 None:intergenic 45.0%
!! GGTGGAACTTGTTGTTGTTG+TGG - chr7_4:55383364-55383383 None:intergenic 45.0%
AGACAGTGCTGTGTTTGTAC+TGG + chr7_4:55383602-55383621 Msa1163920:three_prime_UTR 45.0%
AGTTGGACAGTAGGTTTGGA+TGG - chr7_4:55381886-55381905 None:intergenic 45.0%
!! AGAGAGGTGTTAGGCTTTGA+AGG - chr7_4:55381923-55381942 None:intergenic 45.0%
!! GAAAGAGAGAGAGAGGTGTT+AGG - chr7_4:55381932-55381951 None:intergenic 45.0%
AGAGAGAGAAAGAGAGAGAG+AGG - chr7_4:55381939-55381958 None:intergenic 45.0%
GCTTCCCAAAACTATAGCTG+TGG - chr7_4:55382098-55382117 None:intergenic 45.0%
!! CTTGTTCTTCTAGGGTTCCT+TGG - chr7_4:55382308-55382327 None:intergenic 45.0%
CAAGGAACCCTAGAAGAACA+AGG + chr7_4:55382306-55382325 Msa1163920:intron 45.0%
CTTTGTGCTTTGCAGATCCA+AGG - chr7_4:55382386-55382405 None:intergenic 45.0%
CTTGGATCTGCAAAGCACAA+AGG + chr7_4:55382384-55382403 Msa1163920:five_prime_UTR 45.0%
! ACTGTTGGGACTGTTGATGA+TGG - chr7_4:55382596-55382615 None:intergenic 45.0%
GGCGTTTCTGATTCTCATAG+CGG - chr7_4:55382624-55382643 None:intergenic 45.0%
GGTAGCTATGATGCGAATTG+TGG + chr7_4:55383006-55383025 Msa1163920:intron 45.0%
!! ATGTCCTCTAAGGCAAGCAT+GGG + chr7_4:55383162-55383181 Msa1163920:CDS 45.0%
!! ACTTGTTGTTGTTGTGGTGG+TGG - chr7_4:55383358-55383377 None:intergenic 45.0%
!! GGAACTTGTTGTTGTTGTGG+TGG - chr7_4:55383361-55383380 None:intergenic 45.0%
!! GGTGGAACTTGTTGTTGTTG+TGG - chr7_4:55383364-55383383 None:intergenic 45.0%
AGACAGTGCTGTGTTTGTAC+TGG + chr7_4:55383602-55383621 Msa1163920:three_prime_UTR 45.0%
!!! TATGATTTTTAATATTATTT+TGG + chr7_4:55383034-55383053 Msa1163920:intron 5.0%
!!! TATGATTTTTAATATTATTT+TGG + chr7_4:55383034-55383053 Msa1163920:intron 5.0%
CAGTAGGTTTGGATGGAGAC+AGG - chr7_4:55381879-55381898 None:intergenic 50.0%
GATGGTTGAAGCTGCAGATG+AGG - chr7_4:55382578-55382597 None:intergenic 50.0%
TCATAGCGGCTGCTACTGTT+GGG - chr7_4:55382610-55382629 None:intergenic 50.0%
AGAATCAGAAACGCCGTGAC+TGG + chr7_4:55382629-55382648 Msa1163920:CDS 50.0%
!! GACCAAAGGTGTTCCAGTCA+CGG - chr7_4:55382645-55382664 None:intergenic 50.0%
CACCATGTCCATGTCCTCTA+AGG + chr7_4:55383152-55383171 Msa1163920:CDS 50.0%
TGCCTTAGAGGACATGGACA+TGG - chr7_4:55383157-55383176 None:intergenic 50.0%
CATGCTTGCCTTAGAGGACA+TGG - chr7_4:55383163-55383182 None:intergenic 50.0%
!! CATGTCCTCTAAGGCAAGCA+TGG + chr7_4:55383161-55383180 Msa1163920:CDS 50.0%
!! TGTCCTCTAAGGCAAGCATG+GGG + chr7_4:55383163-55383182 Msa1163920:CDS 50.0%
GGAAGCCTTGATGCACTCAT+AGG + chr7_4:55383184-55383203 Msa1163920:CDS 50.0%
GACGACCTATGAGTGCATCA+AGG - chr7_4:55383192-55383211 None:intergenic 50.0%
GAAGATCACGAACTTCGCGA+AGG - chr7_4:55383288-55383307 None:intergenic 50.0%
TAGGTTTGGATGGAGACAGG+AGG - chr7_4:55381876-55381895 None:intergenic 50.0%
CAGTAGGTTTGGATGGAGAC+AGG - chr7_4:55381879-55381898 None:intergenic 50.0%
GATGGTTGAAGCTGCAGATG+AGG - chr7_4:55382578-55382597 None:intergenic 50.0%
TCATAGCGGCTGCTACTGTT+GGG - chr7_4:55382610-55382629 None:intergenic 50.0%
AGAATCAGAAACGCCGTGAC+TGG + chr7_4:55382629-55382648 Msa1163920:CDS 50.0%
!! GACCAAAGGTGTTCCAGTCA+CGG - chr7_4:55382645-55382664 None:intergenic 50.0%
CACCATGTCCATGTCCTCTA+AGG + chr7_4:55383152-55383171 Msa1163920:CDS 50.0%
TGCCTTAGAGGACATGGACA+TGG - chr7_4:55383157-55383176 None:intergenic 50.0%
CATGCTTGCCTTAGAGGACA+TGG - chr7_4:55383163-55383182 None:intergenic 50.0%
!! CATGTCCTCTAAGGCAAGCA+TGG + chr7_4:55383161-55383180 Msa1163920:CDS 50.0%
!! TGTCCTCTAAGGCAAGCATG+GGG + chr7_4:55383163-55383182 Msa1163920:CDS 50.0%
GGAAGCCTTGATGCACTCAT+AGG + chr7_4:55383184-55383203 Msa1163920:CDS 50.0%
GACGACCTATGAGTGCATCA+AGG - chr7_4:55383192-55383211 None:intergenic 50.0%
GAAGATCACGAACTTCGCGA+AGG - chr7_4:55383288-55383307 None:intergenic 50.0%
CTCATAGCGGCTGCTACTGT+TGG - chr7_4:55382611-55382630 None:intergenic 55.0%
GTCCACCTCTTTCCCTCTCT+AGG + chr7_4:55382683-55382702 Msa1163920:CDS 55.0%
CACCTAGAGAGGGAAAGAGG+TGG - chr7_4:55382688-55382707 None:intergenic 55.0%
CTGCACCTAGAGAGGGAAAG+AGG - chr7_4:55382691-55382710 None:intergenic 55.0%
CTTTCCCTCTCTAGGTGCAG+CGG + chr7_4:55382691-55382710 Msa1163920:CDS 55.0%
!! GGACATGGTGCTGGTGGATT+TGG - chr7_4:55383142-55383161 None:intergenic 55.0%
AGAGGACATGGACATGGTGC+TGG - chr7_4:55383151-55383170 None:intergenic 55.0%
CTTCCCCATGCTTGCCTTAG+AGG - chr7_4:55383169-55383188 None:intergenic 55.0%
CTCACAGCTCGAGCACCAAA+AGG - chr7_4:55383259-55383278 None:intergenic 55.0%
!!! CTTTTGGTGCTCGAGCTGTG+AGG + chr7_4:55383257-55383276 Msa1163920:CDS 55.0%
CAAGTTCCACCACCAGCACA+AGG + chr7_4:55383373-55383392 Msa1163920:CDS 55.0%
GTTGCACTTGCACCTTGTGC+TGG - chr7_4:55383388-55383407 None:intergenic 55.0%
CTCATAGCGGCTGCTACTGT+TGG - chr7_4:55382611-55382630 None:intergenic 55.0%
GTCCACCTCTTTCCCTCTCT+AGG + chr7_4:55382683-55382702 Msa1163920:CDS 55.0%
CACCTAGAGAGGGAAAGAGG+TGG - chr7_4:55382688-55382707 None:intergenic 55.0%
CTGCACCTAGAGAGGGAAAG+AGG - chr7_4:55382691-55382710 None:intergenic 55.0%
CTTTCCCTCTCTAGGTGCAG+CGG + chr7_4:55382691-55382710 Msa1163920:CDS 55.0%
!! GGACATGGTGCTGGTGGATT+TGG - chr7_4:55383142-55383161 None:intergenic 55.0%
AGAGGACATGGACATGGTGC+TGG - chr7_4:55383151-55383170 None:intergenic 55.0%
CTTCCCCATGCTTGCCTTAG+AGG - chr7_4:55383169-55383188 None:intergenic 55.0%
CTCACAGCTCGAGCACCAAA+AGG - chr7_4:55383259-55383278 None:intergenic 55.0%
!!! CTTTTGGTGCTCGAGCTGTG+AGG + chr7_4:55383257-55383276 Msa1163920:CDS 55.0%
CAAGTTCCACCACCAGCACA+AGG + chr7_4:55383373-55383392 Msa1163920:CDS 55.0%
GTTGCACTTGCACCTTGTGC+TGG - chr7_4:55383388-55383407 None:intergenic 55.0%
CGCCGTGACTGGAACACCTT+TGG + chr7_4:55382640-55382659 Msa1163920:CDS 60.0%
TGCACCGCTGCACCTAGAGA+GGG - chr7_4:55382698-55382717 None:intergenic 60.0%
! GGACATGGACATGGTGCTGG+TGG - chr7_4:55383148-55383167 None:intergenic 60.0%
! CTTGCACCTTGTGCTGGTGG+TGG - chr7_4:55383382-55383401 None:intergenic 60.0%
GCACTTGCACCTTGTGCTGG+TGG - chr7_4:55383385-55383404 None:intergenic 60.0%
CGCCGTGACTGGAACACCTT+TGG + chr7_4:55382640-55382659 Msa1163920:CDS 60.0%
TGCACCGCTGCACCTAGAGA+GGG - chr7_4:55382698-55382717 None:intergenic 60.0%
! GGACATGGACATGGTGCTGG+TGG - chr7_4:55383148-55383167 None:intergenic 60.0%
! CTTGCACCTTGTGCTGGTGG+TGG - chr7_4:55383382-55383401 None:intergenic 60.0%
GCACTTGCACCTTGTGCTGG+TGG - chr7_4:55383385-55383404 None:intergenic 60.0%
GTGCACCGCTGCACCTAGAG+AGG - chr7_4:55382699-55382718 None:intergenic 65.0%
GTGCACCGCTGCACCTAGAG+AGG - chr7_4:55382699-55382718 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr7_4 gene 55381875 55383823 55381875 ID=Msa1163920;Name=Msa1163920
chr7_4 mRNA 55381875 55383823 55381875 ID=Msa1163920-mRNA-1;Parent=Msa1163920;Name=Msa1163920-mRNA-1;_AED=0.04;_eAED=0.04;_QI=475|1|1|1|0.5|0.33|3|409|212
chr7_4 exon 55381875 55382223 55381875 ID=Msa1163920-mRNA-1:exon:16169;Parent=Msa1163920-mRNA-1
chr7_4 exon 55382310 55382737 55382310 ID=Msa1163920-mRNA-1:exon:16170;Parent=Msa1163920-mRNA-1
chr7_4 exon 55383078 55383823 55383078 ID=Msa1163920-mRNA-1:exon:16171;Parent=Msa1163920-mRNA-1
chr7_4 five_prime_UTR 55381875 55382223 55381875 ID=Msa1163920-mRNA-1:five_prime_utr;Parent=Msa1163920-mRNA-1
chr7_4 five_prime_UTR 55382310 55382435 55382310 ID=Msa1163920-mRNA-1:five_prime_utr;Parent=Msa1163920-mRNA-1
chr7_4 CDS 55382436 55382737 55382436 ID=Msa1163920-mRNA-1:cds;Parent=Msa1163920-mRNA-1
chr7_4 CDS 55383078 55383414 55383078 ID=Msa1163920-mRNA-1:cds;Parent=Msa1163920-mRNA-1
chr7_4 three_prime_UTR 55383415 55383823 55383415 ID=Msa1163920-mRNA-1:three_prime_utr;Parent=Msa1163920-mRNA-1
Gene Sequence

>Msa1163920

ATGGATTCAATTCAAGAATTTATAGGGACATGTAACAATGAAAACATGACCTGTAACTTCATGAATAACAACAACAACAACAGTATAAGTACAACCACAACAAGCTTAACAACAACAACAACAGCTAGTGGAAGCTCTTCCTCATCTGCAGCTTCAACCATCATCAACAGTCCCAACAGTAGCAGCCGCTATGAGAATCAGAAACGCCGTGACTGGAACACCTTTGGTCAGTATCTCAAGAATCATCGTCCACCTCTTTCCCTCTCTAGGTGCAGCGGTGCACATGTCCTTGAATTCCTAAGGTATTTGGATCAATTTGGGAAGACAAAAGTTCACACACCAATTTGTCCATTTTATGGGCATCCAAATCCACCAGCACCATGTCCATGTCCTCTAAGGCAAGCATGGGGAAGCCTTGATGCACTCATAGGTCGTCTTCGTGCTGCTTTTGAAGAAAATGGAGGAAAACCAGAAACAAATCCTTTTGGTGCTCGAGCTGTGAGGCTCTACCTTCGCGAAGTTCGTGATCTTCAATCAAAAGCAAGAGGTATTAGTTACGAGAAGAAGAAAAGAAAGCGTCCACCACCACAACAACAACAAGTTCCACCACCAGCACAAGGTGCAAGTGCAACTCATTAG
Protein sequence

>Msa1163920

MDSIQEFIGTCNNENMTCNFMNNNNNNSISTTTTSLTTTTTASGSSSSSAASTIINSPNSSSRYENQKRRDWNTFGQYLKNHRPPLSLSRCSGAHVLEFLRYLDQFGKTKVHTPICPFYGHPNPPAPCPCPLRQAWGSLDALIGRLRAAFEENGGKPETNPFGARAVRLYLREVRDLQSKARGISYEKKKRKRPPPQQQQVPPPAQGASATH*