AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1037130


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1037130 MtrunA17_Chr7g0267961 85.356 239 5 2 1 209 219 457 7.67e-145 411
Msa1037130 MtrunA17_Chr5g0395821 70.168 238 42 1 1 209 217 454 2.42e-121 351
Msa1037130 MtrunA17_Chr3g0098921 68.201 239 46 2 1 209 221 459 1.17e-116 339
Msa1037130 MtrunA17_Chr7g0228191 73.214 224 29 2 1 193 106 329 9.45e-114 328
Msa1037130 MtrunA17_Chr7g0235181 69.167 240 38 4 1 209 192 426 2.31e-112 327
Msa1037130 MtrunA17_Chr7g0233651 64.286 238 47 2 1 209 203 431 2.75e-102 302
Msa1037130 MtrunA17_Chr7g0234591 65.417 240 49 3 1 209 200 436 3.20e-102 302
Msa1037130 MtrunA17_Chr2g0330601 65.385 208 64 3 4 207 166 369 8.15e-88 263
Msa1037130 MtrunA17_Chr3g0111961 48.309 207 84 1 3 186 259 465 7.95e-65 207
Msa1037130 MtrunA17_Chr2g0308501 48.148 216 80 4 4 188 99 313 3.43e-63 199
Msa1037130 MtrunA17_Chr2g0330681 58.115 191 32 4 4 163 196 369 6.90e-62 197
Msa1037130 MtrunA17_Chr4g0057131 46.860 207 87 1 4 187 249 455 2.99e-61 198
Msa1037130 MtrunA17_Chr2g0281451 27.429 175 101 5 3 175 97 247 1.19e-11 63.5
Msa1037130 MtrunA17_Chr4g0026671 26.667 180 119 5 3 182 33 199 8.74e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1037130 AT4G33270.1 71.849 238 38 1 1 209 220 457 7.63e-121 350
Msa1037130 AT4G33260.1 71.849 238 38 1 1 209 210 447 6.52e-116 337
Msa1037130 AT5G27570.1 67.083 240 48 3 1 209 211 450 2.47e-106 313
Msa1037130 AT5G27945.1 65.690 239 52 2 1 209 190 428 2.47e-105 310
Msa1037130 AT4G33260.2 69.683 221 38 1 1 192 210 430 2.77e-105 310
Msa1037130 AT5G27080.1 64.583 240 54 3 1 209 203 442 7.01e-102 301
Msa1037130 AT5G26900.1 64.435 239 55 2 1 209 206 444 9.24e-102 301
Msa1037130 AT5G13840.1 48.792 207 83 1 3 186 254 460 8.38e-66 210
Msa1037130 AT5G13840.2 48.792 207 83 1 3 186 254 460 8.38e-66 210
Msa1037130 AT4G22910.1 47.343 207 86 1 4 187 257 463 3.94e-64 206
Msa1037130 AT4G11920.1 44.860 214 95 1 4 194 249 462 1.13e-61 199

Find 64 sgRNAs with CRISPR-Local

Find 76 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGATGAAACTCTTAGATTT+TGG 0.143277 7_1:+73633525 Msa1037130:CDS
GTGGCTTTGCATGCTTCTGC+TGG 0.230906 7_1:-73633563 None:intergenic
CCTTTGGAAGTATCCTTCTA+TGG 0.254046 7_1:+73633415 Msa1037130:CDS
CAGGGTAATCTACTGGCTTC+TGG 0.309255 7_1:+73633236 Msa1037130:intron
GTATCCTTCTATGGTGAAAA+TGG 0.311467 7_1:+73633424 Msa1037130:CDS
AAGGGCTCATTATATGTCTT+TGG 0.358151 7_1:-73633587 None:intergenic
ACGATAATGTTGTTCACATA+TGG 0.371186 7_1:+73633119 Msa1037130:CDS
AATCTACTGGCTTCTGGTGG+AGG 0.376592 7_1:+73633242 Msa1037130:CDS
GTCATCATCTTCAACGAATA+CGG 0.384451 7_1:-73633622 None:intergenic
TTAGTAGCTCACGCTCATTC+TGG 0.385492 7_1:-73633361 None:intergenic
CACTTCGAGGGTGATGTATA+TGG 0.388915 7_1:+73633463 Msa1037130:CDS
TGGCTTTGCATGCTTCTGCT+GGG 0.422084 7_1:-73633562 None:intergenic
CCGAGAACCAGCTTACCCTT+TGG 0.427049 7_1:+73633399 Msa1037130:CDS
CTCACATCGTTGAAACATAC+AGG 0.428220 7_1:+73633029 Msa1037130:CDS
CTACTGGCTTCTGGTGGAGG+TGG 0.431389 7_1:+73633245 Msa1037130:CDS
AACAATCATATTCTGACAAC+AGG 0.439142 7_1:+73632962 Msa1037130:CDS
CTGGCTTCTGGTGGAGGTGG+AGG 0.440261 7_1:+73633248 Msa1037130:CDS
CAAGAGTGGGTTCACTGGCT+TGG 0.451387 7_1:+73632939 Msa1037130:CDS
GATGCCACTGTACACCCATC+TGG 0.480407 7_1:-73633494 None:intergenic
ATCTCAACGGTCACACTTCG+AGG 0.480528 7_1:+73633450 Msa1037130:CDS
CAGAGCAAGAGTGGGTTCAC+TGG 0.481190 7_1:+73632934 Msa1037130:CDS
CCAAAGGGTAAGCTGGTTCT+CGG 0.481991 7_1:-73633399 None:intergenic
TGCAAGTATGTGCTCTGCTA+TGG 0.485347 7_1:+73633336 Msa1037130:CDS
CGATAATGTTGTTCACATAT+GGG 0.487900 7_1:+73633120 Msa1037130:CDS
TGTCCCTCAGGGTAATCTAC+TGG 0.491265 7_1:+73633229 Msa1037130:intron
GGACACAACCAGGAGGTTTG+TGG 0.497262 7_1:+73633052 Msa1037130:CDS
GATACTTCCAAAGGGTAAGC+TGG 0.503616 7_1:-73633406 None:intergenic
GACACAACCAGGAGGTTTGT+GGG 0.510860 7_1:+73633053 Msa1037130:CDS
AACATACAGGGGACACAACC+AGG 0.511067 7_1:+73633042 Msa1037130:CDS
CTCATTATATGTCTTTGGTG+TGG 0.516280 7_1:-73633582 None:intergenic
TATTCTGACAACAGGAGGAA+TGG 0.524698 7_1:+73632970 Msa1037130:CDS
GGGCTCAAGTGGTCACCCTC+AGG 0.528586 7_1:+73633073 Msa1037130:CDS
AGGAGGTTTGTGGGCTCAAG+TGG 0.528883 7_1:+73633062 Msa1037130:CDS
ACTTGAGCCCACAAACCTCC+TGG 0.538177 7_1:-73633060 None:intergenic
TCATCATCTTCAACGAATAC+GGG 0.547121 7_1:-73633621 None:intergenic
GGGTGGACACAGAGCAAGAG+TGG 0.549117 7_1:+73632925 Msa1037130:CDS
AGATTGAATTCAGTTGACAC+TGG 0.560409 7_1:+73633311 Msa1037130:CDS
GGTAATCTACTGGCTTCTGG+TGG 0.565300 7_1:+73633239 Msa1037130:CDS
TATATGGCGCAAAGTCCAGA+TGG 0.565373 7_1:+73633479 Msa1037130:CDS
CGTGAGCTACTAAGCTCACA+TGG 0.568122 7_1:+73633371 Msa1037130:CDS
CTGACAACAGGAGGAATGGA+TGG 0.572412 7_1:+73632974 Msa1037130:CDS
CATAGAAGGATACTTCCAAA+GGG 0.584766 7_1:-73633414 None:intergenic
TCACATATGGGATAGATCTG+TGG 0.596581 7_1:+73633132 Msa1037130:CDS
GGACAACAATTGTCAAGTGG+TGG 0.599505 7_1:+73633094 Msa1037130:CDS
ATACGGGTGAAATTAGCAAA+GGG 0.600657 7_1:-73633605 None:intergenic
TCTCAACGGTCACACTTCGA+GGG 0.613321 7_1:+73633451 Msa1037130:CDS
GTGAAAATGGCAGATCTCAA+CGG 0.613838 7_1:+73633437 Msa1037130:CDS
AATACGGGTGAAATTAGCAA+AGG 0.619999 7_1:-73633606 None:intergenic
CAGCTAAGGGCAATGAAGGG+TGG 0.622963 7_1:+73632908 None:intergenic
CCATAGAAGGATACTTCCAA+AGG 0.623454 7_1:-73633415 None:intergenic
AAGCTGTGGAACACACACAC+TGG 0.630912 7_1:+73633284 Msa1037130:CDS
GTGATCATTGCATTAAGCTG+TGG 0.635919 7_1:+73633270 Msa1037130:CDS
AATCATATTCTGACAACAGG+AGG 0.641326 7_1:+73632965 Msa1037130:CDS
ACTTGACAATTGTTGTCCTG+AGG 0.654097 7_1:-73633089 None:intergenic
TCACATCGTTGAAACATACA+GGG 0.656486 7_1:+73633030 Msa1037130:CDS
CTTGACAATTGTTGTCCTGA+GGG 0.657303 7_1:-73633088 None:intergenic
TCAGGACAACAATTGTCAAG+TGG 0.664718 7_1:+73633091 Msa1037130:CDS
TGGGTGTACAGTGGCATCTG+CGG 0.666837 7_1:+73633499 Msa1037130:CDS
CTGCAGCTAAGGGCAATGAA+GGG 0.668716 7_1:+73632905 None:intergenic
GGTGGACACAGAGCAAGAGT+GGG 0.681424 7_1:+73632926 Msa1037130:CDS
AAGTCCAGATGGGTGTACAG+TGG 0.686450 7_1:+73633490 Msa1037130:CDS
ATATGGCGCAAAGTCCAGAT+GGG 0.692355 7_1:+73633480 Msa1037130:CDS
CACATCGTTGAAACATACAG+GGG 0.758385 7_1:+73633031 Msa1037130:CDS
ATACAGGGGACACAACCAGG+AGG 0.774768 7_1:+73633045 Msa1037130:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTAGATTTTGGAATGTCTTT+GGG + chr7_1:73633537-73633556 Msa1037130:CDS 25.0%
AACAATCATATTCTGACAAC+AGG + chr7_1:73632962-73632981 Msa1037130:CDS 30.0%
ACGATAATGTTGTTCACATA+TGG + chr7_1:73633119-73633138 Msa1037130:CDS 30.0%
CGATAATGTTGTTCACATAT+GGG + chr7_1:73633120-73633139 Msa1037130:CDS 30.0%
! CAGATGAAACTCTTAGATTT+TGG + chr7_1:73633525-73633544 Msa1037130:CDS 30.0%
!!! CTTAGATTTTGGAATGTCTT+TGG + chr7_1:73633536-73633555 Msa1037130:CDS 30.0%
AATCATATTCTGACAACAGG+AGG + chr7_1:73632965-73632984 Msa1037130:CDS 35.0%
TCACATCGTTGAAACATACA+GGG + chr7_1:73633030-73633049 Msa1037130:CDS 35.0%
AGATTGAATTCAGTTGACAC+TGG + chr7_1:73633311-73633330 Msa1037130:CDS 35.0%
CATAGAAGGATACTTCCAAA+GGG - chr7_1:73633417-73633436 None:intergenic 35.0%
GTATCCTTCTATGGTGAAAA+TGG + chr7_1:73633424-73633443 Msa1037130:CDS 35.0%
CTCATTATATGTCTTTGGTG+TGG - chr7_1:73633585-73633604 None:intergenic 35.0%
AAGGGCTCATTATATGTCTT+TGG - chr7_1:73633590-73633609 None:intergenic 35.0%
ATACGGGTGAAATTAGCAAA+GGG - chr7_1:73633608-73633627 None:intergenic 35.0%
AATACGGGTGAAATTAGCAA+AGG - chr7_1:73633609-73633628 None:intergenic 35.0%
TATTCTGACAACAGGAGGAA+TGG + chr7_1:73632970-73632989 Msa1037130:CDS 40.0%
CTCACATCGTTGAAACATAC+AGG + chr7_1:73633029-73633048 Msa1037130:CDS 40.0%
CACATCGTTGAAACATACAG+GGG + chr7_1:73633031-73633050 Msa1037130:CDS 40.0%
CTTGACAATTGTTGTCCTGA+GGG - chr7_1:73633091-73633110 None:intergenic 40.0%
ACTTGACAATTGTTGTCCTG+AGG - chr7_1:73633092-73633111 None:intergenic 40.0%
TCAGGACAACAATTGTCAAG+TGG + chr7_1:73633091-73633110 Msa1037130:CDS 40.0%
TCACATATGGGATAGATCTG+TGG + chr7_1:73633132-73633151 Msa1037130:CDS 40.0%
GTGATCATTGCATTAAGCTG+TGG + chr7_1:73633270-73633289 Msa1037130:CDS 40.0%
CCATAGAAGGATACTTCCAA+AGG - chr7_1:73633418-73633437 None:intergenic 40.0%
!! CCTTTGGAAGTATCCTTCTA+TGG + chr7_1:73633415-73633434 Msa1037130:CDS 40.0%
!!! TCTGCCATTTTCACCATAGA+AGG - chr7_1:73633431-73633450 None:intergenic 40.0%
GTGAAAATGGCAGATCTCAA+CGG + chr7_1:73633437-73633456 Msa1037130:CDS 40.0%
GGACAACAATTGTCAAGTGG+TGG + chr7_1:73633094-73633113 Msa1037130:CDS 45.0%
CTTCAAATTCACCTACCCGA+TGG + chr7_1:73633158-73633177 Msa1037130:intron 45.0%
AAGCCAGTAGATTACCCTGA+GGG - chr7_1:73633235-73633254 None:intergenic 45.0%
TGCAAGTATGTGCTCTGCTA+TGG + chr7_1:73633336-73633355 Msa1037130:CDS 45.0%
TTAGTAGCTCACGCTCATTC+TGG - chr7_1:73633364-73633383 None:intergenic 45.0%
GATACTTCCAAAGGGTAAGC+TGG - chr7_1:73633409-73633428 None:intergenic 45.0%
CACTTCGAGGGTGATGTATA+TGG + chr7_1:73633463-73633482 Msa1037130:CDS 45.0%
TATATGGCGCAAAGTCCAGA+TGG + chr7_1:73633479-73633498 Msa1037130:CDS 45.0%
ATATGGCGCAAAGTCCAGAT+GGG + chr7_1:73633480-73633499 Msa1037130:CDS 45.0%
CTGACAACAGGAGGAATGGA+TGG + chr7_1:73632974-73632993 Msa1037130:CDS 50.0%
AACATACAGGGGACACAACC+AGG + chr7_1:73633042-73633061 Msa1037130:CDS 50.0%
GACACAACCAGGAGGTTTGT+GGG + chr7_1:73633053-73633072 Msa1037130:CDS 50.0%
CTCAAACCTGTGAAGCCATC+GGG - chr7_1:73633176-73633195 None:intergenic 50.0%
CCTCAAACCTGTGAAGCCAT+CGG - chr7_1:73633177-73633196 None:intergenic 50.0%
GGAACACATAGCTGCTGTGA+AGG + chr7_1:73633195-73633214 Msa1037130:intron 50.0%
TGTCCCTCAGGGTAATCTAC+TGG + chr7_1:73633229-73633248 Msa1037130:intron 50.0%
GAAGCCAGTAGATTACCCTG+AGG - chr7_1:73633236-73633255 None:intergenic 50.0%
!! CAGGGTAATCTACTGGCTTC+TGG + chr7_1:73633236-73633255 Msa1037130:intron 50.0%
!! GGTAATCTACTGGCTTCTGG+TGG + chr7_1:73633239-73633258 Msa1037130:CDS 50.0%
!! AATCTACTGGCTTCTGGTGG+AGG + chr7_1:73633242-73633261 Msa1037130:CDS 50.0%
AAGCTGTGGAACACACACAC+TGG + chr7_1:73633284-73633303 Msa1037130:CDS 50.0%
CGTGAGCTACTAAGCTCACA+TGG + chr7_1:73633371-73633390 Msa1037130:CDS 50.0%
! CCAAAGGGTAAGCTGGTTCT+CGG - chr7_1:73633402-73633421 None:intergenic 50.0%
ATCTCAACGGTCACACTTCG+AGG + chr7_1:73633450-73633469 Msa1037130:CDS 50.0%
TCTCAACGGTCACACTTCGA+GGG + chr7_1:73633451-73633470 Msa1037130:CDS 50.0%
AAGTCCAGATGGGTGTACAG+TGG + chr7_1:73633490-73633509 Msa1037130:CDS 50.0%
! TGGCTTTGCATGCTTCTGCT+GGG - chr7_1:73633565-73633584 None:intergenic 50.0%
GGTGGACACAGAGCAAGAGT+GGG + chr7_1:73632926-73632945 Msa1037130:CDS 55.0%
! CAGAGCAAGAGTGGGTTCAC+TGG + chr7_1:73632934-73632953 Msa1037130:CDS 55.0%
! CAAGAGTGGGTTCACTGGCT+TGG + chr7_1:73632939-73632958 Msa1037130:CDS 55.0%
ATACAGGGGACACAACCAGG+AGG + chr7_1:73633045-73633064 Msa1037130:CDS 55.0%
GGACACAACCAGGAGGTTTG+TGG + chr7_1:73633052-73633071 Msa1037130:CDS 55.0%
ACTTGAGCCCACAAACCTCC+TGG - chr7_1:73633063-73633082 None:intergenic 55.0%
AGGAGGTTTGTGGGCTCAAG+TGG + chr7_1:73633062-73633081 Msa1037130:CDS 55.0%
AACCTGTGAAGCCATCGGGT+AGG - chr7_1:73633172-73633191 None:intergenic 55.0%
CCGATGGCTTCACAGGTTTG+AGG + chr7_1:73633174-73633193 Msa1037130:intron 55.0%
! CATAGCTGCTGTGAAGGCAC+TGG + chr7_1:73633201-73633220 Msa1037130:intron 55.0%
CCGAGAACCAGCTTACCCTT+TGG + chr7_1:73633399-73633418 Msa1037130:CDS 55.0%
GATGCCACTGTACACCCATC+TGG - chr7_1:73633497-73633516 None:intergenic 55.0%
TGGGTGTACAGTGGCATCTG+CGG + chr7_1:73633499-73633518 Msa1037130:CDS 55.0%
! GTGGCTTTGCATGCTTCTGC+TGG - chr7_1:73633566-73633585 None:intergenic 55.0%
GGGTGGACACAGAGCAAGAG+TGG + chr7_1:73632925-73632944 Msa1037130:CDS 60.0%
CACCTACCCGATGGCTTCAC+AGG + chr7_1:73633167-73633186 Msa1037130:intron 60.0%
!! CTGCTGTGAAGGCACTGGCT+TGG + chr7_1:73633206-73633225 Msa1037130:intron 60.0%
!! CACTGGCTTGGTGTCCCTCA+GGG + chr7_1:73633218-73633237 Msa1037130:intron 60.0%
!! CTACTGGCTTCTGGTGGAGG+TGG + chr7_1:73633245-73633264 Msa1037130:CDS 60.0%
GGGCTCAAGTGGTCACCCTC+AGG + chr7_1:73633073-73633092 Msa1037130:CDS 65.0%
!! GCACTGGCTTGGTGTCCCTC+AGG + chr7_1:73633217-73633236 Msa1037130:intron 65.0%
CTGGCTTCTGGTGGAGGTGG+AGG + chr7_1:73633248-73633267 Msa1037130:CDS 65.0%
Chromosome Type Strat End Strand Name
chr7_1 gene 73632920 73633634 73632920 ID=Msa1037130;Name=Msa1037130
chr7_1 mRNA 73632920 73633634 73632920 ID=Msa1037130-mRNA-1;Parent=Msa1037130;Name=Msa1037130-mRNA-1;_AED=0.07;_eAED=0.07;_QI=0|0|0|1|1|1|2|0|209
chr7_1 exon 73632920 73633153 73632920 ID=Msa1037130-mRNA-1:exon:18172;Parent=Msa1037130-mRNA-1
chr7_1 exon 73633239 73633634 73633239 ID=Msa1037130-mRNA-1:exon:18173;Parent=Msa1037130-mRNA-1
chr7_1 CDS 73632920 73633153 73632920 ID=Msa1037130-mRNA-1:cds;Parent=Msa1037130-mRNA-1
chr7_1 CDS 73633239 73633634 73633239 ID=Msa1037130-mRNA-1:cds;Parent=Msa1037130-mRNA-1
Gene Sequence

>Msa1037130

ATGAAGGGTGGACACAGAGCAAGAGTGGGTTCACTGGCTTGGAACAATCATATTCTGACAACAGGAGGAATGGATGGTAAAGTTGTAAACAATGATGTTAGAGTGAGATCTCACATCGTTGAAACATACAGGGGACACAACCAGGAGGTTTGTGGGCTCAAGTGGTCACCCTCAGGACAACAATTGTCAAGTGGTGGAAACGATAATGTTGTTCACATATGGGATAGATCTGTGGGTAATCTACTGGCTTCTGGTGGAGGTGGAGGTGATCATTGCATTAAGCTGTGGAACACACACACTGGTGCAAGATTGAATTCAGTTGACACTGGATTGCAAGTATGTGCTCTGCTATGGAACCAGAATGAGCGTGAGCTACTAAGCTCACATGGCTTCACCGAGAACCAGCTTACCCTTTGGAAGTATCCTTCTATGGTGAAAATGGCAGATCTCAACGGTCACACTTCGAGGGTGATGTATATGGCGCAAAGTCCAGATGGGTGTACAGTGGCATCTGCGGCTGCAGATGAAACTCTTAGATTTTGGAATGTCTTTGGGAGCCCAGCAGAAGCATGCAAAGCCACACCAAAGACATATAATGAGCCCTTTGCTAATTTCACCCGTATTCGTTGA

Protein sequence

>Msa1037130

MKGGHRARVGSLAWNNHILTTGGMDGKVVNNDVRVRSHIVETYRGHNQEVCGLKWSPSGQQLSSGGNDNVVHIWDRSVGNLLASGGGGGDHCIKLWNTHTGARLNSVDTGLQVCALLWNQNERELLSSHGFTENQLTLWKYPSMVKMADLNGHTSRVMYMAQSPDGCTVASAAADETLRFWNVFGSPAEACKATPKTYNEPFANFTRIR*