AlfalfaGEDB | Msa1043060

M. sativa cultivar zhongmu-4 / Msa1043060

KEGG: -
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1043060	XP_003623159.1	94.872	156	8	0	1	156	1	156	2.52e-93	277

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1043060	G7L5L0	94.872	156	8	0	1	156	1	156	1.21e-93	277

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa1006710	Msa1043060	0.837214	5.930188e-57	-8.615850e-47
Msa1043060	Msa1150720	0.877311	6.956573e-69	-8.615850e-47
Msa1043060	Msa1448620	0.804325	2.280545e-49	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1043060	MtrunA17_Chr7g0240431	94.872	156	8	0	1	156	1	156	2.32e-97	277
Msa1043060	MtrunA17_Chr4g0008731	55.625	160	63	4	1	156	1	156	7.18e-50	157
Msa1043060	MtrunA17_Chr4g0008721	44.375	160	76	5	1	156	1	151	3.81e-33	114

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1043060	AT5G02020.1	54.717	159	59	4	1	156	1	149	2.32e-37	125
Msa1043060	AT5G59080.2	43.827	162	58	4	1	156	1	135	2.80e-25	94.4
Msa1043060	AT5G59080.1	43.827	162	58	4	1	156	1	135	2.80e-25	94.4
Msa1043060	AT2G39855.2	42.143	140	59	6	21	156	21	142	1.12e-21	85.5
Msa1043060	AT2G39855.4	42.143	140	59	6	21	156	21	142	1.12e-21	85.5
Msa1043060	AT5G02020.2	49.206	126	54	3	1	123	1	119	1.96e-20	82.0
Msa1043060	AT3G55646.1	42.857	126	53	5	32	156	39	146	2.23e-18	77.0
Msa1043060	AT3G46880.2	36.943	157	57	4	1	156	1	116	4.52e-17	72.8
Msa1043060	AT2G39855.3	61.905	63	18	2	95	156	60	117	5.00e-17	72.8

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Find 57 sgRNAs with CRISPR-Local

Find 142 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGGAGAGACACGTGAAA+AGG	0.505203	7_2:-901528	None:intergenic
AAAATAGAAATTACAATATA+GGG	0.223199	7_2:-904130	None:intergenic
AAACTTGTTTACAGTGGCGG+TGG	0.585550	7_2:+901625	Msa1043060:five_prime_UTR
AAAGTGCGTCAAGAGGAAAC+TGG	0.578629	7_2:+903301	Msa1043060:CDS
AAATAGAAATTACAATATAG+GGG	0.566541	7_2:-904129	None:intergenic
AACTCAGAACAAAACAAATA+AGG	0.412533	7_2:+902823	Msa1043060:CDS
AACTCCAAAATTGCTACTCA+AGG	0.556191	7_2:+902302	Msa1043060:CDS
AAGAGAACAGAAGTTTGAAA+AGG	0.475721	7_2:-901545	None:intergenic
AATGCAGATTACATCAGTAA+GGG	0.615541	7_2:+902788	Msa1043060:intron
ACAAACAGAGTGAAGAAAGA+AGG	0.555856	7_2:-901572	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAGAGAACAGAAGTTTGAAA+AGG	-	chr7_2:901548-901567	None:intergenic	30.0%
TGTAAACAAGTTTGCAAATG+AGG	-	chr7_2:901618-901637	None:intergenic	30.0%
TGTTGAAGCAAAAAAATGGA+AGG	+	chr7_2:901701-901720	Msa1043060:exon	30.0%
ACACAAAAAACCTTAGAAGA+TGG	-	chr7_2:901842-901861	None:intergenic	30.0%
TTTGTGATGATTTAGGTGTT+TGG	+	chr7_2:902227-902246	Msa1043060:intron	30.0%
CATTGAGATCAAGGAAAATA+GGG	-	chr7_2:902614-902633	None:intergenic	30.0%
ACATTGAGATCAAGGAAAAT+AGG	-	chr7_2:902615-902634	None:intergenic	30.0%
TAATGCAGATTACATCAGTA+AGG	+	chr7_2:902787-902806	Msa1043060:intron	30.0%
AATGCAGATTACATCAGTAA+GGG	+	chr7_2:902788-902807	Msa1043060:intron	30.0%
CATAATAGATTGATGAGCTA+AGG	-	chr7_2:902892-902911	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr7_2	gene	901501	904196	901501	ID=Msa1043060;Name=Msa1043060
chr7_2	mRNA	901501	904196	901501	ID=Msa1043060-mRNA-1;Parent=Msa1043060;Name=Msa1043060-mRNA-1;_AED=0.17;_eAED=0.17;_QI=215\|1\|1\|1\|0.8\|0.66\|6\|437\|156
chr7_2	exon	901501	901850	901501	ID=Msa1043060-mRNA-1:exon:130;Parent=Msa1043060-mRNA-1
chr7_2	exon	902242	902323	902242	ID=Msa1043060-mRNA-1:exon:131;Parent=Msa1043060-mRNA-1
chr7_2	exon	902795	902970	902795	ID=Msa1043060-mRNA-1:exon:132;Parent=Msa1043060-mRNA-1
chr7_2	exon	903270	903330	903270	ID=Msa1043060-mRNA-1:exon:133;Parent=Msa1043060-mRNA-1
chr7_2	exon	903436	903513	903436	ID=Msa1043060-mRNA-1:exon:134;Parent=Msa1043060-mRNA-1
chr7_2	exon	903821	904196	903821	ID=Msa1043060-mRNA-1:exon:135;Parent=Msa1043060-mRNA-1
chr7_2	five_prime_UTR	901501	901715	901501	ID=Msa1043060-mRNA-1:five_prime_utr;Parent=Msa1043060-mRNA-1
chr7_2	CDS	901716	901850	901716	ID=Msa1043060-mRNA-1:cds;Parent=Msa1043060-mRNA-1
chr7_2	CDS	902242	902323	902242	ID=Msa1043060-mRNA-1:cds;Parent=Msa1043060-mRNA-1
chr7_2	CDS	902795	902970	902795	ID=Msa1043060-mRNA-1:cds;Parent=Msa1043060-mRNA-1
chr7_2	CDS	903270	903330	903270	ID=Msa1043060-mRNA-1:cds;Parent=Msa1043060-mRNA-1
chr7_2	CDS	903436	903452	903436	ID=Msa1043060-mRNA-1:cds;Parent=Msa1043060-mRNA-1
chr7_2	three_prime_UTR	903453	903513	903453	ID=Msa1043060-mRNA-1:three_prime_utr;Parent=Msa1043060-mRNA-1
chr7_2	three_prime_UTR	903821	904196	903821	ID=Msa1043060-mRNA-1:three_prime_utr;Parent=Msa1043060-mRNA-1

Gene Sequence

>Msa1043060

ATGGAAGGAAGGAAACAATTTGGTTCTTCTTCTTCTGAAACTTCTTCTTTCACTTCTGAACTCTTTGGTTCCAATGACTTGCATAAATCCTCTTCTTCTGGAATTTTTGACTCTATTTTCTCTCCATCTTCTAAGGTGTTTGGAAGAGAATCTCTGCGCTCACCAATGAATGGGAAAACTGCCACTGAAGAATCAAACTCCAAAATTGCTACTCAAGATTACATCAGTAAGGGAAATGATGGTGAAACTCAGAACAAAACAAATAAGGATATGAGTTATATTTATCAAGAACAAAGAGTTCAACCATGTCACCTTAGCTCATCAATCTATTATGGTGGCCAAGACGTATATTCTCGTCCTCAGAGTACACAAGATTCAGGATTGAACACTATGTACAAGAAAGATGGAGGAGAAGATGATTCAGAAAGTGCGTCAAGAGGAAACTGGTGGCAAGGATCTCTCTATTATTAA

Protein sequence

>Msa1043060

MEGRKQFGSSSSETSSFTSELFGSNDLHKSSSSGIFDSIFSPSSKVFGRESLRSPMNGKTATEESNSKIATQDYISKGNDGETQNKTNKDMSYIYQEQRVQPCHLSSSIYYGGQDVYSRPQSTQDSGLNTMYKKDGGEDDSESASRGNWWQGSLYY*