AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1047860

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1047860 MtrunA17_Chr1g0210011 90.323 31 3 0 50 80 492 522 2.25e-15 69.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 41 sgRNAs with CRISPR-Local

Find 207 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCACCTGATAAGGTTATTT+TGG 0.129147 7_2:-8994928 Msa1047860:three_prime_UTR
ATTGAATGCTCAAATGTATT+TGG 0.176146 7_2:-8996967 Msa1047860:CDS
GAGTAAATGTATGGAGGATA+TGG 0.234869 7_2:-8998597 Msa1047860:CDS
CTACAGGTTGAAGAGGATTT+TGG 0.270337 7_2:-8998324 Msa1047860:intron
TACAAAGTACTGTATTTATA+TGG 0.327615 7_2:+8995048 None:intergenic
GTAAACGAAGAGCACGTTAT+AGG 0.334243 7_2:+8994811 None:intergenic
ATGACCAAAATAACCTTATC+AGG 0.354107 7_2:+8994924 None:intergenic
TGCTCAAATGTATTTGGATA+TGG 0.360208 7_2:-8996961 Msa1047860:CDS
ATTGGAGAAGGAAACCATTC+AGG 0.390426 7_2:+8996921 None:intergenic
GTGATTGACTTCACCTGATA+AGG 0.392851 7_2:-8994937 Msa1047860:three_prime_UTR
ATGACTCACTGAATCTCTTT+CGG 0.393981 7_2:+8998718 None:intergenic
ATTTGGATATGGAGGACAAT+GGG 0.404111 7_2:-8996950 Msa1047860:CDS
GTGATCAGAGAGTAAATGTA+TGG 0.429517 7_2:-8998606 Msa1047860:CDS
AGTAAATGTATGGAGGATAT+GGG 0.431529 7_2:-8998596 Msa1047860:CDS
GTCACTAATCCTACACCAAT+GGG 0.440630 7_2:+8998413 None:intergenic
ACTCATGTATCGTGCGGAAA+CGG 0.451317 7_2:-8998756 None:intergenic
TACATTTGAGCATTCAATAG+TGG 0.454695 7_2:+8996972 None:intergenic
TTGCGTCCCGTGCTGTCGCA+CGG 0.465383 7_2:+8994788 None:intergenic
TGCGTCCCGTGCTGTCGCAC+GGG 0.518842 7_2:+8994789 None:intergenic
TCTAAGAGTTTCTATTCTCT+CGG 0.530513 7_2:-8995013 Msa1047860:three_prime_UTR
CGTTTACCCGTGCGACAGCA+CGG 0.542824 7_2:-8994795 Msa1047860:three_prime_UTR
GTTTACCCGTGCGACAGCAC+GGG 0.547248 7_2:-8994794 Msa1047860:three_prime_UTR
CCAGAGCAGGGAAGATGAGA+GGG 0.566974 7_2:-8998635 Msa1047860:intron
TGTCACTAATCCTACACCAA+TGG 0.567188 7_2:+8998412 None:intergenic
ACACCATAAAATACAACGAA+AGG 0.568318 7_2:+8994850 None:intergenic
TTGATCATCATGCATAGGAT+AGG 0.574768 7_2:-8994973 Msa1047860:three_prime_UTR
TAGAGTTGATCATCATGCAT+AGG 0.578321 7_2:-8994978 Msa1047860:three_prime_UTR
ATCAGAGAGTAAATGTATGG+AGG 0.579509 7_2:-8998603 Msa1047860:CDS
GCCAGAGCAGGGAAGATGAG+AGG 0.579773 7_2:-8998636 Msa1047860:intron
TATTTGGATATGGAGGACAA+TGG 0.581621 7_2:-8996951 Msa1047860:CDS
CACTAATCCTACACCAATGG+GGG 0.584380 7_2:+8998415 None:intergenic
GTATCGTGCGGAAACGGAGA+CGG 0.589747 7_2:-8998750 Msa1047860:CDS
GTAAATGTATGGAGGATATG+GGG 0.601739 7_2:-8998595 Msa1047860:intron
TTTGGATATGGAGGACAATG+GGG 0.604747 7_2:-8996949 Msa1047860:CDS
TCAAATGTATTTGGATATGG+AGG 0.605535 7_2:-8996958 Msa1047860:CDS
GAGCAGGGAAGATGAGAGGG+AGG 0.607437 7_2:-8998632 Msa1047860:CDS
GCGGAAACGGAGACGGCAAG+CGG 0.617742 7_2:-8998743 Msa1047860:CDS
TTCACTACTCATGTATCGTG+CGG 0.646340 7_2:-8998762 None:intergenic
ACAATGGGGAGATACCTGAA+TGG 0.664243 7_2:-8996935 Msa1047860:CDS
TCACTAATCCTACACCAATG+GGG 0.680015 7_2:+8998414 None:intergenic
ACTAATCCTACACCAATGGG+GGG 0.711279 7_2:+8998416 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CTAAAAAAATTCAATTGTTT+GGG - chr7_2:8994990-8995009 Msa1047860:three_prime_UTR 15.0%
!! TAAAAAAATTCAATTGTTTG+GGG - chr7_2:8994991-8995010 Msa1047860:three_prime_UTR 15.0%
!! TTATAGAGAGAAAAATTATA+AGG + chr7_2:8995899-8995918 None:intergenic 15.0%
!!! TATAATTTTTCTCTCTATAA+AGG - chr7_2:8995899-8995918 Msa1047860:intron 15.0%
!!! AATTTATCTGAATTTGTTTA+TGG - chr7_2:8996343-8996362 Msa1047860:intron 15.0%
!! ACAATTAAAAAGAAAATTAC+AGG + chr7_2:8996392-8996411 None:intergenic 15.0%
!!! ACTTTTTGATTAAGATTTTT+GGG - chr7_2:8997199-8997218 Msa1047860:intron 15.0%
!!! GGATTAAATTTTTAAAAAAG+AGG - chr7_2:8997665-8997684 Msa1047860:intron 15.0%
!!! CCAAACAATTGAATTTTTTT+AGG + chr7_2:8994992-8995011 None:intergenic 20.0%
!! CCTAAAAAAATTCAATTGTT+TGG - chr7_2:8994989-8995008 Msa1047860:three_prime_UTR 20.0%
!!! TGTTTTTTATTTTTCACTAC+AGG - chr7_2:8995195-8995214 Msa1047860:intron 20.0%
!!! TGTAATTTGAGTTTAAATTG+AGG - chr7_2:8995369-8995388 Msa1047860:intron 20.0%
!! CTATTGTTTACTTCTTTATA+TGG - chr7_2:8995614-8995633 Msa1047860:intron 20.0%
!! AATAAATATGCAAAGTAGAA+TGG - chr7_2:8995711-8995730 Msa1047860:intron 20.0%
!!! TTCCTTTTCATTATTTTAGT+GGG - chr7_2:8996031-8996050 Msa1047860:intron 20.0%
!! GAATTTATTGAAATGTGAAT+TGG - chr7_2:8996181-8996200 Msa1047860:intron 20.0%
!! AAATAAGAAGAAGAGAAAAA+AGG + chr7_2:8996311-8996330 None:intergenic 20.0%
!! CAAGCATGATATATAAAATT+AGG + chr7_2:8996466-8996485 None:intergenic 20.0%
!!! CTAATTTTATATATCATGCT+TGG - chr7_2:8996464-8996483 Msa1047860:intron 20.0%
!! AGTGATAGTAATATAATCTA+TGG - chr7_2:8996751-8996770 Msa1047860:intron 20.0%
!!! GACTTTTTGATTAAGATTTT+TGG - chr7_2:8997198-8997217 Msa1047860:intron 20.0%
!! TCAATTAATGAAATCATTAG+AGG + chr7_2:8997581-8997600 None:intergenic 20.0%
!! GATTAAATAATCTTTATAGC+TGG - chr7_2:8997735-8997754 Msa1047860:intron 20.0%
!! TGATATATAAATGCTTTATG+TGG - chr7_2:8997911-8997930 Msa1047860:intron 20.0%
!!! TTATTAGGGACATTTTTTAT+TGG + chr7_2:8998128-8998147 None:intergenic 20.0%
!! AATAAAAAATGTCCCTAATA+AGG - chr7_2:8998127-8998146 Msa1047860:intron 20.0%
!!! TACAAAGTACTGTATTTATA+TGG + chr7_2:8998490-8998509 None:intergenic 20.0%
!!! TATTTTGGTCATAAGTATTT+TGG - chr7_2:8998622-8998641 Msa1047860:CDS 20.0%
!!! AAAATGATTGATGATGTTTG+TGG - chr7_2:8994874-8994893 Msa1047860:three_prime_UTR 25.0%
! TTCCTGAGAGAAAAAAAAAA+GGG - chr7_2:8995028-8995047 Msa1047860:three_prime_UTR 25.0%
! AATCAAAACTGAAATTGTAG+GGG + chr7_2:8995099-8995118 None:intergenic 25.0%
! GAATCAAAACTGAAATTGTA+GGG + chr7_2:8995100-8995119 None:intergenic 25.0%
! GATTAGTGACAATTTATGTT+TGG - chr7_2:8995135-8995154 Msa1047860:intron 25.0%
! TTATGCTAATTTCGTCAATA+GGG - chr7_2:8995268-8995287 Msa1047860:intron 25.0%
! AAAATTCGATCATTTAAGCA+AGG + chr7_2:8995641-8995660 None:intergenic 25.0%
! ATATAACATAACGACAAACT+CGG + chr7_2:8995674-8995693 None:intergenic 25.0%
! TTAACACAAATCAGAATAGT+TGG + chr7_2:8995837-8995856 None:intergenic 25.0%
! TAGAGAGAAAAATTATAAGG+AGG + chr7_2:8995896-8995915 None:intergenic 25.0%
!!! CTTCCTTTTCATTATTTTAG+TGG - chr7_2:8996030-8996049 Msa1047860:intron 25.0%
! AACCCACTAAAATAATGAAA+AGG + chr7_2:8996036-8996055 None:intergenic 25.0%
! TTATTGAAATGTGAATTGGA+TGG - chr7_2:8996185-8996204 Msa1047860:intron 25.0%
! TTTGGAGGTTAATTTCTATT+TGG - chr7_2:8996513-8996532 Msa1047860:intron 25.0%
! ATTGAATGCTCAAATGTATT+TGG - chr7_2:8996568-8996587 Msa1047860:intron 25.0%
! TGTTTGTTTACCTGAAAAAT+TGG + chr7_2:8996635-8996654 None:intergenic 25.0%
!!! TTTTTCAGGTAAACAAACAA+AGG - chr7_2:8996636-8996655 Msa1047860:intron 25.0%
! CATTTCATATATTACTGTTC+AGG - chr7_2:8997063-8997082 Msa1047860:intron 25.0%
!!! TTTGTGATTTAAAGCATTGT+TGG - chr7_2:8997297-8997316 Msa1047860:intron 25.0%
! CTAAATATAATCCTACTCAT+AGG - chr7_2:8997359-8997378 Msa1047860:intron 25.0%
! ATGTAAGCATAATATCATCT+TGG - chr7_2:8997403-8997422 Msa1047860:intron 25.0%
! TACATTCAACAATGAACAAT+AGG - chr7_2:8997521-8997540 Msa1047860:intron 25.0%
! TTAATGAAATCATTAGAGGT+AGG + chr7_2:8997577-8997596 None:intergenic 25.0%
! TGAAAATAACAGATGATCAA+AGG - chr7_2:8997598-8997617 Msa1047860:intron 25.0%
! TATTAGGAGTTTATAGAGTA+TGG + chr7_2:8997805-8997824 None:intergenic 25.0%
! TGGAAAATTATGTGCATATT+AGG + chr7_2:8997821-8997840 None:intergenic 25.0%
!!! TTATTTATCTGGCATTGATA+TGG + chr7_2:8997954-8997973 None:intergenic 25.0%
! TTAACACCTCATTATTTATC+TGG + chr7_2:8997965-8997984 None:intergenic 25.0%
! GCCTTTCAAATTGTAAAATT+AGG - chr7_2:8998071-8998090 Msa1047860:intron 25.0%
!! GCCTAATTTTACAATTTGAA+AGG + chr7_2:8998075-8998094 None:intergenic 25.0%
! TAATGTTGGATTCCTTATTA+GGG + chr7_2:8998142-8998161 None:intergenic 25.0%
! AAAAATGTGTATCAAGTACT+TGG + chr7_2:8998431-8998450 None:intergenic 25.0%
!! TTTCCTTTCGTTGTATTTTA+TGG - chr7_2:8998682-8998701 Msa1047860:intron 25.0%
! TTTTTGAACTCATGACCAAA+GGG + chr7_2:8994857-8994876 None:intergenic 30.0%
!!! TTTTTTGAACTCATGACCAA+AGG + chr7_2:8994858-8994877 None:intergenic 30.0%
AGTAAATGTATGGAGGATAT+GGG - chr7_2:8994939-8994958 Msa1047860:three_prime_UTR 30.0%
! TTCAATTGTTTGGGGTTAAT+TGG - chr7_2:8994999-8995018 Msa1047860:three_prime_UTR 30.0%
! TCAATTGTTTGGGGTTAATT+GGG - chr7_2:8995000-8995019 Msa1047860:three_prime_UTR 30.0%
CTTCCTGAGAGAAAAAAAAA+AGG - chr7_2:8995027-8995046 Msa1047860:three_prime_UTR 30.0%
ATCAAAACTGAAATTGTAGG+GGG + chr7_2:8995098-8995117 None:intergenic 30.0%
GGAATCAAAACTGAAATTGT+AGG + chr7_2:8995101-8995120 None:intergenic 30.0%
CTTATGCTAATTTCGTCAAT+AGG - chr7_2:8995267-8995286 Msa1047860:intron 30.0%
TATGCTAATTTCGTCAATAG+GGG - chr7_2:8995269-8995288 Msa1047860:intron 30.0%
!!! TTTTTTTATTGTTCGTGACC+AGG - chr7_2:8995309-8995328 Msa1047860:intron 30.0%
TACAATTCCAAAATCTTACC+TGG + chr7_2:8995330-8995349 None:intergenic 30.0%
!!! TGCGATTTTTTTTCGTTTGT+TGG - chr7_2:8995454-8995473 Msa1047860:intron 30.0%
!!! GATTTTTTTTCGTTTGTTGG+AGG - chr7_2:8995457-8995476 Msa1047860:intron 30.0%
GAGTTTGTCGTTATGTTATA+TGG - chr7_2:8995673-8995692 Msa1047860:intron 30.0%
! TCGTTATGTTATATGGCTTT+TGG - chr7_2:8995680-8995699 Msa1047860:intron 30.0%
! CGTTATGTTATATGGCTTTT+GGG - chr7_2:8995681-8995700 Msa1047860:intron 30.0%
ATATGCAAAGTAGAATGGAA+AGG - chr7_2:8995716-8995735 Msa1047860:intron 30.0%
! GCATCACAAAAAAATGCTTT+TGG - chr7_2:8995795-8995814 Msa1047860:intron 30.0%
GAGGAGTGAAATAATGAATT+TGG + chr7_2:8995877-8995896 None:intergenic 30.0%
AAGGAAAATAATATGCAGGT+TGG + chr7_2:8995982-8996001 None:intergenic 30.0%
!!! TTTAGTGGGTTGAATCATTT+TGG - chr7_2:8996045-8996064 Msa1047860:intron 30.0%
CCTTACATGAAAATCATTTG+AGG + chr7_2:8996276-8996295 None:intergenic 30.0%
! CCTCAAATGATTTTCATGTA+AGG - chr7_2:8996273-8996292 Msa1047860:intron 30.0%
GTATTAGTCCATCTTATGTT+TGG - chr7_2:8996410-8996429 Msa1047860:intron 30.0%
ATTCACTTCCAAACATAAGA+TGG + chr7_2:8996421-8996440 None:intergenic 30.0%
TACATTTGAGCATTCAATAG+TGG + chr7_2:8996566-8996585 None:intergenic 30.0%
TGCTCAAATGTATTTGGATA+TGG - chr7_2:8996574-8996593 Msa1047860:intron 30.0%
TCAAATGTATTTGGATATGG+AGG - chr7_2:8996577-8996596 Msa1047860:intron 30.0%
TTTACCTGAAAAATTGGAGA+AGG + chr7_2:8996629-8996648 None:intergenic 30.0%
TTTGAATGTATTGCATGCAT+TGG - chr7_2:8996848-8996867 Msa1047860:intron 30.0%
TTGAATGTATCCACGTAAAT+TGG - chr7_2:8996961-8996980 Msa1047860:CDS 30.0%
ATGTCTTCAATATCAAGTCT+CGG - chr7_2:8997016-8997035 Msa1047860:intron 30.0%
AGACAAAAAAACCTATGAGT+AGG + chr7_2:8997373-8997392 None:intergenic 30.0%
CTTAATGAAGGTTTCTTCAT+TGG - chr7_2:8997644-8997663 Msa1047860:intron 30.0%
TCAATGCCAGATAAATAATG+AGG - chr7_2:8997956-8997975 Msa1047860:intron 30.0%
GTAATGTTGGATTCCTTATT+AGG + chr7_2:8998143-8998162 None:intergenic 30.0%
AGTGGCAATAATAGTAATGT+TGG + chr7_2:8998156-8998175 None:intergenic 30.0%
CAACATTAGTATTAGTTGCA+GGG + chr7_2:8998245-8998264 None:intergenic 30.0%
TCTAAGAGTTTCTATTCTCT+CGG - chr7_2:8998522-8998541 Msa1047860:intron 30.0%
! TTCACCTGATAAGGTTATTT+TGG - chr7_2:8998607-8998626 Msa1047860:CDS 30.0%
ATGACCAAAATAACCTTATC+AGG + chr7_2:8998614-8998633 None:intergenic 30.0%
ACACCATAAAATACAACGAA+AGG + chr7_2:8998688-8998707 None:intergenic 30.0%
! CGTTGTATTTTATGGTGTTT+TGG - chr7_2:8998690-8998709 Msa1047860:intron 30.0%
ATGACTCACTGAATCTCTTT+CGG + chr7_2:8994820-8994839 None:intergenic 35.0%
AAAGAGATTCAGTGAGTCAT+TGG - chr7_2:8994820-8994839 Msa1047860:three_prime_UTR 35.0%
AAGAGATTCAGTGAGTCATT+GGG - chr7_2:8994821-8994840 Msa1047860:three_prime_UTR 35.0%
TTGGGATAATATCTTCCCTT+TGG - chr7_2:8994839-8994858 Msa1047860:three_prime_UTR 35.0%
GTGATCAGAGAGTAAATGTA+TGG - chr7_2:8994929-8994948 Msa1047860:three_prime_UTR 35.0%
ATCAGAGAGTAAATGTATGG+AGG - chr7_2:8994932-8994951 Msa1047860:three_prime_UTR 35.0%
GAGTAAATGTATGGAGGATA+TGG - chr7_2:8994938-8994957 Msa1047860:three_prime_UTR 35.0%
GTAAATGTATGGAGGATATG+GGG - chr7_2:8994940-8994959 Msa1047860:three_prime_UTR 35.0%
!!! CACCCTTTTTTTTTTCTCTC+AGG + chr7_2:8995033-8995052 None:intergenic 35.0%
CAGAACAGAATTACAGAAGA+GGG + chr7_2:8995062-8995081 None:intergenic 35.0%
ACAGAACAGAATTACAGAAG+AGG + chr7_2:8995063-8995082 None:intergenic 35.0%
TCTTCTGTAATTCTGTTCTG+TGG - chr7_2:8995062-8995081 Msa1047860:three_prime_UTR 35.0%
AAAACAGTGATGATAGTCAG+TGG + chr7_2:8995181-8995200 None:intergenic 35.0%
!! TTTTTCACTACAGGTTGAAG+AGG - chr7_2:8995204-8995223 Msa1047860:intron 35.0%
TGACGAAATTAGCATAAGAG+TGG + chr7_2:8995265-8995284 None:intergenic 35.0%
ACATGTGCAATTGAGTTTCA+AGG - chr7_2:8995569-8995588 Msa1047860:intron 35.0%
!! GAGTTTCAAGGTTTTGTAGT+TGG - chr7_2:8995581-8995600 Msa1047860:intron 35.0%
! TATGTTATATGGCTTTTGGG+AGG - chr7_2:8995684-8995703 Msa1047860:intron 35.0%
!!! GAATGGAAAGGTGTTTTGTT+TGG - chr7_2:8995728-8995747 Msa1047860:intron 35.0%
TCAAGTTTGCATAGCTTATG+CGG - chr7_2:8995773-8995792 Msa1047860:intron 35.0%
GCAGAAGGAAAATAATATGC+AGG + chr7_2:8995986-8996005 None:intergenic 35.0%
AATCAAATGACTGAAGCAGA+AGG + chr7_2:8996001-8996020 None:intergenic 35.0%
! TATATCATGCTTGGTTCTCT+TGG - chr7_2:8996473-8996492 Msa1047860:intron 35.0%
!!! GTAGTTTTGACGAACATGTT+TGG - chr7_2:8996495-8996514 Msa1047860:intron 35.0%
TTCTATTTGGCCATTGTTCT+TGG - chr7_2:8996526-8996545 Msa1047860:intron 35.0%
TATTTGGATATGGAGGACAA+TGG - chr7_2:8996584-8996603 Msa1047860:intron 35.0%
ATTTGGATATGGAGGACAAT+GGG - chr7_2:8996585-8996604 Msa1047860:intron 35.0%
! GTTTCCTTCTCCAATTTTTC+AGG - chr7_2:8996622-8996641 Msa1047860:intron 35.0%
AAGTGTAGAGCTATAACCAA+TGG + chr7_2:8996675-8996694 None:intergenic 35.0%
TTAAAGCTTACCAGAATTGC+AGG - chr7_2:8997094-8997113 Msa1047860:intron 35.0%
ATCTGATGCATTTGTGTGAA+GGG - chr7_2:8997123-8997142 Msa1047860:intron 35.0%
! TGAAACAGGACAAACTGTTT+TGG - chr7_2:8997229-8997248 Msa1047860:intron 35.0%
! GAAACAGGACAAACTGTTTT+GGG - chr7_2:8997230-8997249 Msa1047860:intron 35.0%
TGAACTCATATACCTGTTGA+AGG + chr7_2:8997265-8997284 None:intergenic 35.0%
ATAATCCTACTCATAGCCAT+AGG - chr7_2:8997453-8997472 Msa1047860:intron 35.0%
ACTAATAGCAGATCTGTGAT+TGG - chr7_2:8997621-8997640 Msa1047860:intron 35.0%
!! ATCTGTGATTGGCTTAATGA+AGG - chr7_2:8997632-8997651 Msa1047860:intron 35.0%
GTTATTATAGTGTGCACATG+AGG - chr7_2:8997779-8997798 Msa1047860:intron 35.0%
ATTAGTATTAGTTGCAGGGA+TGG + chr7_2:8998241-8998260 None:intergenic 35.0%
CCAACATTAGTATTAGTTGC+AGG + chr7_2:8998246-8998265 None:intergenic 35.0%
CCTGCAACTAATACTAATGT+TGG - chr7_2:8998243-8998262 Msa1047860:intron 35.0%
! TAGAGTTGATCATCATGCAT+AGG - chr7_2:8998557-8998576 Msa1047860:intron 35.0%
TTGATCATCATGCATAGGAT+AGG - chr7_2:8998562-8998581 Msa1047860:intron 35.0%
TCACTAATCCTACACCAATG+GGG + chr7_2:8995124-8995143 None:intergenic 40.0%
GTCACTAATCCTACACCAAT+GGG + chr7_2:8995125-8995144 None:intergenic 40.0%
TGTCACTAATCCTACACCAA+TGG + chr7_2:8995126-8995145 None:intergenic 40.0%
!! CTACAGGTTGAAGAGGATTT+TGG - chr7_2:8995211-8995230 Msa1047860:intron 40.0%
!! GTTGAAGAGGATTTTGGTCA+TGG - chr7_2:8995217-8995236 Msa1047860:intron 40.0%
! TTCGTGACCAGGTAAGATTT+TGG - chr7_2:8995320-8995339 Msa1047860:intron 40.0%
AGCGACTATGAAAGTGAAGT+AGG - chr7_2:8996141-8996160 Msa1047860:intron 40.0%
!! GTTTTGACGAACATGTTTGG+AGG - chr7_2:8996498-8996517 Msa1047860:intron 40.0%
TTTGGATATGGAGGACAATG+GGG - chr7_2:8996586-8996605 Msa1047860:intron 40.0%
ATTGGAGAAGGAAACCATTC+AGG + chr7_2:8996617-8996636 None:intergenic 40.0%
AGGATACTTCATGCTTCCAT+TGG - chr7_2:8996656-8996675 Msa1047860:intron 40.0%
! TGTATTGCATGCATTGGTGT+CGG - chr7_2:8996854-8996873 Msa1047860:intron 40.0%
TGAACACAACAATGCACAGA+CGG - chr7_2:8996898-8996917 Msa1047860:intron 40.0%
GTATCCACGTAAATTGGTGT+CGG - chr7_2:8996967-8996986 Msa1047860:CDS 40.0%
! TATCCACGTAAATTGGTGTC+GGG - chr7_2:8996968-8996987 Msa1047860:CDS 40.0%
!! TCGGTGCTAGATAGAGTATA+AGG - chr7_2:8997035-8997054 Msa1047860:intron 40.0%
CATCTGATGCATTTGTGTGA+AGG - chr7_2:8997122-8997141 Msa1047860:intron 40.0%
!! TTGGTGCTAGACAAAAAGCT+TGG - chr7_2:8997316-8997335 Msa1047860:intron 40.0%
! TGGTGCTAGACAAAAAGCTT+GGG - chr7_2:8997317-8997336 Msa1047860:intron 40.0%
! CTTGGGTGTTGTGATATCTT+AGG - chr7_2:8997334-8997353 Msa1047860:intron 40.0%
TACATTGAGCACACAACCTA+TGG + chr7_2:8997472-8997491 None:intergenic 40.0%
ATAGGTTGTGTGCTCAATGT+AGG - chr7_2:8997471-8997490 Msa1047860:intron 40.0%
AGAGGACAAAAGTCTGTCAT+TGG - chr7_2:8997683-8997702 Msa1047860:intron 40.0%
ACGATTTGAGCTACACAAAG+TGG + chr7_2:8997841-8997860 None:intergenic 40.0%
ATTGCCACTAATTCCTCTAG+AGG - chr7_2:8998167-8998186 Msa1047860:intron 40.0%
! TATGCCTCTAGAGGAATTAG+TGG + chr7_2:8998174-8998193 None:intergenic 40.0%
AGTTATGAGTATGCCTCTAG+AGG + chr7_2:8998183-8998202 None:intergenic 40.0%
! GTGATTGACTTCACCTGATA+AGG - chr7_2:8998598-8998617 Msa1047860:CDS 40.0%
! GTAAACGAAGAGCACGTTAT+AGG + chr7_2:8998727-8998746 None:intergenic 40.0%
!!! TCAGTTTTGATTCCCCCCAT+TGG - chr7_2:8995107-8995126 Msa1047860:exon 45.0%
ACTAATCCTACACCAATGGG+GGG + chr7_2:8995122-8995141 None:intergenic 45.0%
CACTAATCCTACACCAATGG+GGG + chr7_2:8995123-8995142 None:intergenic 45.0%
CCATCTTCTGCCAAGAACAA+TGG + chr7_2:8996539-8996558 None:intergenic 45.0%
CCATTGTTCTTGGCAGAAGA+TGG - chr7_2:8996536-8996555 Msa1047860:intron 45.0%
ACAATGGGGAGATACCTGAA+TGG - chr7_2:8996600-8996619 Msa1047860:intron 45.0%
AGATGCAATGCCTGCAATTC+TGG + chr7_2:8997107-8997126 None:intergenic 45.0%
! TTTTGGGCTGACGTTGAAAC+AGG - chr7_2:8997215-8997234 Msa1047860:intron 45.0%
!! CAAACTGTTTTGGGTCCTCT+CGG - chr7_2:8997239-8997258 Msa1047860:intron 45.0%
CACAACCTATGGCTATGAGT+AGG + chr7_2:8997461-8997480 None:intergenic 45.0%
GTATTAGTTGCAGGGATGGT+GGG + chr7_2:8998237-8998256 None:intergenic 45.0%
AGTATTAGTTGCAGGGATGG+TGG + chr7_2:8998238-8998257 None:intergenic 45.0%
ATGTTTGTGGTAGCCAGAGC+AGG - chr7_2:8994887-8994906 Msa1047860:three_prime_UTR 50.0%
TGTTTGTGGTAGCCAGAGCA+GGG - chr7_2:8994888-8994907 Msa1047860:three_prime_UTR 50.0%
TTGATTCCCCCCATTGGTGT+AGG - chr7_2:8995113-8995132 Msa1047860:exon 50.0%
TATACCTGTTGAAGGCCGAG+AGG + chr7_2:8997257-8997276 None:intergenic 50.0%
GCTCATATGTCGTGTGCTCA+AGG - chr7_2:8997546-8997565 Msa1047860:intron 50.0%
GTATCGTGCGGAAACGGAGA+CGG - chr7_2:8994785-8994804 Msa1047860:three_prime_UTR 55.0%
CCAGAGCAGGGAAGATGAGA+GGG - chr7_2:8994900-8994919 Msa1047860:three_prime_UTR 55.0%
GTGCCCGACACCAATTTACG+TGG + chr7_2:8996974-8996993 None:intergenic 55.0%
GCCAGAGCAGGGAAGATGAG+AGG - chr7_2:8994899-8994918 Msa1047860:three_prime_UTR 60.0%
CCCTCTCATCTTCCCTGCTC+TGG + chr7_2:8994903-8994922 None:intergenic 60.0%
GAGCAGGGAAGATGAGAGGG+AGG - chr7_2:8994903-8994922 Msa1047860:three_prime_UTR 60.0%
!! AGTTGCAGGGATGGTGGGCT+AGG + chr7_2:8998232-8998251 None:intergenic 60.0%
CGTTTACCCGTGCGACAGCA+CGG - chr7_2:8998740-8998759 Msa1047860:CDS 60.0%
GTTTACCCGTGCGACAGCAC+GGG - chr7_2:8998741-8998760 Msa1047860:CDS 60.0%
GCGGAAACGGAGACGGCAAG+CGG - chr7_2:8994792-8994811 Msa1047860:three_prime_UTR 65.0%
GGGTCCTCTCGGCCTTCAAC+AGG - chr7_2:8997250-8997269 Msa1047860:intron 65.0%
TTGCGTCCCGTGCTGTCGCA+CGG + chr7_2:8998750-8998769 None:intergenic 65.0%
TGCGTCCCGTGCTGTCGCAC+GGG + chr7_2:8998749-8998768 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr7_2 gene 8994783 8998774 8994783 ID=Msa1047860;Name=Msa1047860
chr7_2 mRNA 8994783 8998774 8994783 ID=Msa1047860-mRNA-1;Parent=Msa1047860;Name=Msa1047860-mRNA-1;_AED=0.42;_eAED=0.42;_QI=0|0.2|0.5|0.83|0.6|0.83|6|335|87
chr7_2 exon 8994783 8995141 8994783 ID=Msa1047860-mRNA-1:exon:2415;Parent=Msa1047860-mRNA-1
chr7_2 exon 8996914 8997005 8996914 ID=Msa1047860-mRNA-1:exon:2414;Parent=Msa1047860-mRNA-1
chr7_2 exon 8998319 8998340 8998319 ID=Msa1047860-mRNA-1:exon:2413;Parent=Msa1047860-mRNA-1
chr7_2 exon 8998427 8998446 8998427 ID=Msa1047860-mRNA-1:exon:2412;Parent=Msa1047860-mRNA-1
chr7_2 exon 8998596 8998653 8998596 ID=Msa1047860-mRNA-1:exon:2411;Parent=Msa1047860-mRNA-1
chr7_2 exon 8998727 8998774 8998727 ID=Msa1047860-mRNA-1:exon:2410;Parent=Msa1047860-mRNA-1
chr7_2 CDS 8998727 8998774 8998727 ID=Msa1047860-mRNA-1:cds;Parent=Msa1047860-mRNA-1
chr7_2 CDS 8998596 8998653 8998596 ID=Msa1047860-mRNA-1:cds;Parent=Msa1047860-mRNA-1
chr7_2 CDS 8998427 8998446 8998427 ID=Msa1047860-mRNA-1:cds;Parent=Msa1047860-mRNA-1
chr7_2 CDS 8998319 8998340 8998319 ID=Msa1047860-mRNA-1:cds;Parent=Msa1047860-mRNA-1
chr7_2 CDS 8996914 8997005 8996914 ID=Msa1047860-mRNA-1:cds;Parent=Msa1047860-mRNA-1
chr7_2 CDS 8995118 8995141 8995118 ID=Msa1047860-mRNA-1:cds;Parent=Msa1047860-mRNA-1
chr7_2 three_prime_UTR 8994783 8995117 8994783 ID=Msa1047860-mRNA-1:three_prime_utr;Parent=Msa1047860-mRNA-1
Gene Sequence

>Msa1047860

ATGTATCGTGCGGAAACGGAGACGGCAAGCGGAACCGAAAGAGATTCAAGCAGGGAAGATGAGAGGGAGGTTAGTGATCAGAGAGTAAATGTATGGAGGATATGGGTTTTGATTCCCCCCATTGGTGTTGAAGAGGATTTTGGTCATGAAGATGGAGTACCACTATTGAATGCTCAAATGTATTTGGATATGGAGGACAATGGGGAGATACCTGAATGGTTTCCTTCTCCAATTTTTCAGCTTTCAATTCTACCAAGTACTTGA
Protein sequence

>Msa1047860

MYRAETETASGTERDSSREDEREVSDQRVNVWRIWVLIPPIGVEEDFGHEDGVPLLNAQMYLDMEDNGEIPEWFPSPIFQLSILPST*