AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1050450

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1050450 MtrunA17_Chr8g0341721 92.982 171 9 1 1 168 41 211 1.84e-117 333
Msa1050450 MtrunA17_Chr8g0341701 83.626 171 25 1 1 168 41 211 2.25e-105 302
Msa1050450 MtrunA17_Chr8g0341741 81.977 172 27 2 1 168 41 212 1.64e-102 295
Msa1050450 MtrunA17_Chr8g0341731 80.814 172 29 2 1 168 41 212 6.93e-102 293
Msa1050450 MtrunA17_Chr2g0330511 78.363 171 33 2 1 168 40 209 2.21e-97 282
Msa1050450 MtrunA17_Chr2g0309111 76.608 171 37 1 1 168 84 254 7.78e-95 277
Msa1050450 MtrunA17_Chr2g0330491 61.176 170 63 1 1 167 40 209 9.18e-74 222
Msa1050450 MtrunA17_Chr8g0389271 58.721 172 67 3 1 168 38 209 4.77e-69 209
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1050450 AT1G17710.1 70.760 171 46 1 1 167 43 213 1.84e-88 260
Msa1050450 AT1G17710.2 69.231 169 48 1 3 167 13 181 1.14e-84 249
Msa1050450 AT1G73010.1 68.023 172 50 2 1 167 50 221 1.22e-84 251
Msa1050450 AT4G29530.1 55.422 166 70 1 1 162 40 205 2.31e-67 205
Msa1050450 AT4G29530.2 54.938 162 69 1 8 165 36 197 1.00e-63 196

Find 54 sgRNAs with CRISPR-Local

Find 151 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGTCAAATGTGATCCTTTA+AGG 0.035104 7_2:-15167102 Msa1050450:three_prime_UTR
GATATTATTCTGTGATTAAT+TGG 0.161270 7_2:+15167724 None:intergenic
TGCTCTCAAAATTGGTTCTT+TGG 0.208038 7_2:+15168179 None:intergenic
TATGCCTTTCAATTCTCTTA+TGG 0.235071 7_2:-15167940 Msa1050450:intron
TCTCTCAGTGCTCTCAAAAT+TGG 0.235886 7_2:+15168171 None:intergenic
TTGATTGTGATAGTGATAAT+TGG 0.238396 7_2:-15168006 Msa1050450:five_prime_UTR
ATTTCCATGGCGTTCCTTAA+AGG 0.239410 7_2:+15167088 None:intergenic
ATTAAATCAGCTTATGCATT+AGG 0.247679 7_2:-15167697 Msa1050450:CDS
CGCCATGGAAATTCTTTATA+GGG 0.264710 7_2:-15167077 Msa1050450:three_prime_UTR
ACGCCATGGAAATTCTTTAT+AGG 0.279084 7_2:-15167078 Msa1050450:three_prime_UTR
TAGTGATAATTGGGTGAATT+AGG 0.294368 7_2:-15167996 Msa1050450:five_prime_UTR
ATTATACCTTGCACATGTTT+GGG 0.294621 7_2:+15167488 None:intergenic
GATTATACCTTGCACATGTT+TGG 0.298306 7_2:+15167487 None:intergenic
ATCTACCTTGGTGATGGTGC+TGG 0.308894 7_2:-15167330 Msa1050450:CDS
TTAAATCAGCTTATGCATTA+GGG 0.371086 7_2:-15167696 Msa1050450:CDS
CACAAGAAATAGAAGACAAA+TGG 0.381654 7_2:+15168145 None:intergenic
ATGATGGAGCTTCATTCAAA+TGG 0.391230 7_2:-15167787 Msa1050450:CDS
TCTACCTTGGTGATGGTGCT+GGG 0.415344 7_2:-15167329 Msa1050450:CDS
CCTTCTTGATTAACATAACC+AGG 0.424126 7_2:+15167574 None:intergenic
ATGCATTAGGGTGTGATTTG+AGG 0.437272 7_2:-15167684 Msa1050450:CDS
CACACTGGAAAATTCTTCCT+TGG 0.458582 7_2:+15167261 None:intergenic
TGATTGTGATAGTGATAATT+GGG 0.461891 7_2:-15168005 Msa1050450:five_prime_UTR
TCTGAGATTAACACTAATCC+TGG 0.477118 7_2:-15167592 Msa1050450:CDS
AACAAGAGGTTTATCTACCT+TGG 0.481752 7_2:-15167342 Msa1050450:CDS
AATGCTATTGAAATTAAGTA+AGG 0.481831 7_2:+15167006 None:intergenic
ACAAGAAATAGAAGACAAAT+GGG 0.488463 7_2:+15168146 None:intergenic
AACCTCTTCAATCTCTTCGA+TGG 0.499828 7_2:+15167759 None:intergenic
CCTGGTTATGTTAATCAAGA+AGG 0.499879 7_2:-15167574 Msa1050450:CDS
CCTTCTCTAAGTCTCAAGCT+AGG 0.506175 7_2:+15167297 None:intergenic
TGATGGAGCTTCATTCAAAT+GGG 0.508401 7_2:-15167786 Msa1050450:CDS
GCTTTGTTGAAGTCATGATA+AGG 0.514128 7_2:+15167535 None:intergenic
AACCCTATAAAGAATTTCCA+TGG 0.519898 7_2:+15167075 None:intergenic
GACTTCAACAAAGCTTCTCA+TGG 0.521531 7_2:-15167526 Msa1050450:CDS
TTTACAGGACAGAATGATGA+TGG 0.525460 7_2:-15167803 Msa1050450:intron
AAGAGAATTGAAAGGCATAG+TGG 0.528235 7_2:+15167944 None:intergenic
AGGTTTATCTACCTTGGTGA+TGG 0.549393 7_2:-15167336 Msa1050450:CDS
AAGGAGTTGATTGAACAAAT+CGG 0.557882 7_2:+15167968 None:intergenic
AGAGAATTGAAAGGCATAGT+GGG 0.565487 7_2:+15167945 None:intergenic
CTACCTTGGTGATGGTGCTG+GGG 0.569867 7_2:-15167328 Msa1050450:CDS
AGATTGAAGAGGTTCTTCAA+AGG 0.570752 7_2:-15167750 Msa1050450:CDS
AATTGAAAGGCATAGTGGGA+AGG 0.577724 7_2:+15167949 None:intergenic
CTTCTCTAAGTCTCAAGCTA+GGG 0.579387 7_2:+15167298 None:intergenic
TTATACCTTGCACATGTTTG+GGG 0.581671 7_2:+15167489 None:intergenic
AAGGTGACTTTGTGATGCCA+AGG 0.594145 7_2:-15167278 Msa1050450:CDS
TATACCTTGCACATGTTTGG+GGG 0.606803 7_2:+15167490 None:intergenic
TGATCCTTTAAGGAACGCCA+TGG 0.615430 7_2:-15167092 Msa1050450:three_prime_UTR
AACCATCGAAGAGATTGAAG+AGG 0.624606 7_2:-15167761 Msa1050450:CDS
CATACCATAAGAGAATTGAA+AGG 0.628950 7_2:+15167936 None:intergenic
TTTGAGTGGTGAGAAAGTGA+AGG 0.640246 7_2:+15168085 None:intergenic
TTGCATATCAAATCCCACAC+TGG 0.647687 7_2:+15167246 None:intergenic
GATTGTTAGTGATGCTAATG+CGG 0.651747 7_2:-15167662 Msa1050450:CDS
CCTAGCTTGAGACTTAGAGA+AGG 0.660953 7_2:-15167297 Msa1050450:CDS
AGTGTGGGATTTGATATGCA+AGG 0.670478 7_2:-15167244 Msa1050450:CDS
AGTCCCCAGCACCATCACCA+AGG 0.694728 7_2:+15167325 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATAAGTAATTTTTTTTTGTA+GGG + chr7_2:15168144-15168163 None:intergenic 10.0%
!!! TATAAGTAATTTTTTTTTGT+AGG + chr7_2:15168145-15168164 None:intergenic 10.0%
!!! ATAAGTAATTTTTTTTTGTA+GGG + chr7_2:15168144-15168163 None:intergenic 10.0%
!!! TATAAGTAATTTTTTTTTGT+AGG + chr7_2:15168145-15168164 None:intergenic 10.0%
!!! AAACAAAATTTATTTTAGCA+GGG + chr7_2:15168176-15168195 None:intergenic 15.0%
!!! TAAACAAAATTTATTTTAGC+AGG + chr7_2:15168177-15168196 None:intergenic 15.0%
!!! AAACAAAATTTATTTTAGCA+GGG + chr7_2:15168176-15168195 None:intergenic 15.0%
!!! TAAACAAAATTTATTTTAGC+AGG + chr7_2:15168177-15168196 None:intergenic 15.0%
!!! ACATTTTTTTTTTCTTTGAG+TGG + chr7_2:15167050-15167069 None:intergenic 20.0%
!!! ATTAACTTGTGTTTTGATTT+TGG - chr7_2:15167290-15167309 Msa1050450:CDS 20.0%
!! GATATTATTCTGTGATTAAT+TGG + chr7_2:15167397-15167416 None:intergenic 20.0%
!!! GTATTGAATATCAATTTTTG+AGG - chr7_2:15167941-15167960 Msa1050450:CDS 20.0%
!!! TATAGGGTTAATTATGTTTT+TGG - chr7_2:15168057-15168076 Msa1050450:five_prime_UTR 20.0%
!! AATGCTATTGAAATTAAGTA+AGG + chr7_2:15168115-15168134 None:intergenic 20.0%
!!! ACATTTTTTTTTTCTTTGAG+TGG + chr7_2:15167050-15167069 None:intergenic 20.0%
!!! ATTAACTTGTGTTTTGATTT+TGG - chr7_2:15167290-15167309 Msa1050450:CDS 20.0%
!! GATATTATTCTGTGATTAAT+TGG + chr7_2:15167397-15167416 None:intergenic 20.0%
!!! GTATTGAATATCAATTTTTG+AGG - chr7_2:15167941-15167960 Msa1050450:CDS 20.0%
!!! TATAGGGTTAATTATGTTTT+TGG - chr7_2:15168057-15168076 Msa1050450:five_prime_UTR 20.0%
!! AATGCTATTGAAATTAAGTA+AGG + chr7_2:15168115-15168134 None:intergenic 20.0%
! ACAAGAAATAGAAGACAAAT+GGG + chr7_2:15166975-15166994 None:intergenic 25.0%
!! TTGATTGTGATAGTGATAAT+TGG - chr7_2:15167112-15167131 Msa1050450:three_prime_UTR 25.0%
!! TGATTGTGATAGTGATAATT+GGG - chr7_2:15167113-15167132 Msa1050450:three_prime_UTR 25.0%
!!! GTTTTGATTTTGGAATTTAC+AGG - chr7_2:15167300-15167319 Msa1050450:CDS 25.0%
! ATTAAATCAGCTTATGCATT+AGG - chr7_2:15167421-15167440 Msa1050450:intron 25.0%
! TTAAATCAGCTTATGCATTA+GGG - chr7_2:15167422-15167441 Msa1050450:intron 25.0%
!!! AACACAATTTTGAAGCATTT+GGG - chr7_2:15167487-15167506 Msa1050450:intron 25.0%
!!! TCTTGTTCGAAATTGATTTT+TGG + chr7_2:15167763-15167782 None:intergenic 25.0%
! AAAAATCAATTTCGAACAAG+AGG - chr7_2:15167762-15167781 Msa1050450:CDS 25.0%
!! TATAAAGTCGCAATTTTGTA+TGG + chr7_2:15168084-15168103 None:intergenic 25.0%
! ACAAGAAATAGAAGACAAAT+GGG + chr7_2:15166975-15166994 None:intergenic 25.0%
!! TTGATTGTGATAGTGATAAT+TGG - chr7_2:15167112-15167131 Msa1050450:three_prime_UTR 25.0%
!! TGATTGTGATAGTGATAATT+GGG - chr7_2:15167113-15167132 Msa1050450:three_prime_UTR 25.0%
!!! GTTTTGATTTTGGAATTTAC+AGG - chr7_2:15167300-15167319 Msa1050450:CDS 25.0%
! ATTAAATCAGCTTATGCATT+AGG - chr7_2:15167421-15167440 Msa1050450:intron 25.0%
! TTAAATCAGCTTATGCATTA+GGG - chr7_2:15167422-15167441 Msa1050450:intron 25.0%
!!! AACACAATTTTGAAGCATTT+GGG - chr7_2:15167487-15167506 Msa1050450:intron 25.0%
!!! TCTTGTTCGAAATTGATTTT+TGG + chr7_2:15167763-15167782 None:intergenic 25.0%
! AAAAATCAATTTCGAACAAG+AGG - chr7_2:15167762-15167781 Msa1050450:CDS 25.0%
!! TATAAAGTCGCAATTTTGTA+TGG + chr7_2:15168084-15168103 None:intergenic 25.0%
CACAAGAAATAGAAGACAAA+TGG + chr7_2:15166976-15166995 None:intergenic 30.0%
!!! TGAACGTGTTTTTTCATTCT+GGG - chr7_2:15166994-15167013 Msa1050450:three_prime_UTR 30.0%
TAGTGATAATTGGGTGAATT+AGG - chr7_2:15167122-15167141 Msa1050450:three_prime_UTR 30.0%
! AAGGAGTTGATTGAACAAAT+CGG + chr7_2:15167153-15167172 None:intergenic 30.0%
TATGCCTTTCAATTCTCTTA+TGG - chr7_2:15167178-15167197 Msa1050450:three_prime_UTR 30.0%
CATACCATAAGAGAATTGAA+AGG + chr7_2:15167185-15167204 None:intergenic 30.0%
!!! CAACACAATTTTGAAGCATT+TGG - chr7_2:15167486-15167505 Msa1050450:intron 30.0%
ATTATACCTTGCACATGTTT+GGG + chr7_2:15167633-15167652 None:intergenic 30.0%
TTGTCAAATGTGATCCTTTA+AGG - chr7_2:15168016-15168035 Msa1050450:five_prime_UTR 30.0%
AACCCTATAAAGAATTTCCA+TGG + chr7_2:15168046-15168065 None:intergenic 30.0%
CACAAGAAATAGAAGACAAA+TGG + chr7_2:15166976-15166995 None:intergenic 30.0%
!!! TGAACGTGTTTTTTCATTCT+GGG - chr7_2:15166994-15167013 Msa1050450:three_prime_UTR 30.0%
TAGTGATAATTGGGTGAATT+AGG - chr7_2:15167122-15167141 Msa1050450:three_prime_UTR 30.0%
! AAGGAGTTGATTGAACAAAT+CGG + chr7_2:15167153-15167172 None:intergenic 30.0%
TATGCCTTTCAATTCTCTTA+TGG - chr7_2:15167178-15167197 Msa1050450:three_prime_UTR 30.0%
CATACCATAAGAGAATTGAA+AGG + chr7_2:15167185-15167204 None:intergenic 30.0%
!!! CAACACAATTTTGAAGCATT+TGG - chr7_2:15167486-15167505 Msa1050450:intron 30.0%
ATTATACCTTGCACATGTTT+GGG + chr7_2:15167633-15167652 None:intergenic 30.0%
TTGTCAAATGTGATCCTTTA+AGG - chr7_2:15168016-15168035 Msa1050450:five_prime_UTR 30.0%
AACCCTATAAAGAATTTCCA+TGG + chr7_2:15168046-15168065 None:intergenic 30.0%
! TGCTCTCAAAATTGGTTCTT+TGG + chr7_2:15166942-15166961 None:intergenic 35.0%
!!! GTGAACGTGTTTTTTCATTC+TGG - chr7_2:15166993-15167012 Msa1050450:three_prime_UTR 35.0%
! AGAGAATTGAAAGGCATAGT+GGG + chr7_2:15167176-15167195 None:intergenic 35.0%
! AAGAGAATTGAAAGGCATAG+TGG + chr7_2:15167177-15167196 None:intergenic 35.0%
TTTACAGGACAGAATGATGA+TGG - chr7_2:15167315-15167334 Msa1050450:CDS 35.0%
! ATGATGGAGCTTCATTCAAA+TGG - chr7_2:15167331-15167350 Msa1050450:CDS 35.0%
TGATGGAGCTTCATTCAAAT+GGG - chr7_2:15167332-15167351 Msa1050450:CDS 35.0%
AGATTGAAGAGGTTCTTCAA+AGG - chr7_2:15167368-15167387 Msa1050450:CDS 35.0%
GATTGTTAGTGATGCTAATG+CGG - chr7_2:15167456-15167475 Msa1050450:intron 35.0%
! TCTGAGATTAACACTAATCC+TGG - chr7_2:15167526-15167545 Msa1050450:CDS 35.0%
CCTTCTTGATTAACATAACC+AGG + chr7_2:15167547-15167566 None:intergenic 35.0%
CCTGGTTATGTTAATCAAGA+AGG - chr7_2:15167544-15167563 Msa1050450:CDS 35.0%
! GCTTTGTTGAAGTCATGATA+AGG + chr7_2:15167586-15167605 None:intergenic 35.0%
TTATACCTTGCACATGTTTG+GGG + chr7_2:15167632-15167651 None:intergenic 35.0%
GATTATACCTTGCACATGTT+TGG + chr7_2:15167634-15167653 None:intergenic 35.0%
AACAAGAGGTTTATCTACCT+TGG - chr7_2:15167776-15167795 Msa1050450:CDS 35.0%
! AAGGAAGAATTTTCCAGTGT+GGG - chr7_2:15167859-15167878 Msa1050450:intron 35.0%
ACGCCATGGAAATTCTTTAT+AGG - chr7_2:15168040-15168059 Msa1050450:five_prime_UTR 35.0%
CGCCATGGAAATTCTTTATA+GGG - chr7_2:15168041-15168060 Msa1050450:five_prime_UTR 35.0%
! CAAAATTGGTTCTTTGGCTT+AGG + chr7_2:15166936-15166955 None:intergenic 35.0%
! TGCTCTCAAAATTGGTTCTT+TGG + chr7_2:15166942-15166961 None:intergenic 35.0%
!!! GTGAACGTGTTTTTTCATTC+TGG - chr7_2:15166993-15167012 Msa1050450:three_prime_UTR 35.0%
! AGAGAATTGAAAGGCATAGT+GGG + chr7_2:15167176-15167195 None:intergenic 35.0%
! AAGAGAATTGAAAGGCATAG+TGG + chr7_2:15167177-15167196 None:intergenic 35.0%
TTTACAGGACAGAATGATGA+TGG - chr7_2:15167315-15167334 Msa1050450:CDS 35.0%
! ATGATGGAGCTTCATTCAAA+TGG - chr7_2:15167331-15167350 Msa1050450:CDS 35.0%
TGATGGAGCTTCATTCAAAT+GGG - chr7_2:15167332-15167351 Msa1050450:CDS 35.0%
AGATTGAAGAGGTTCTTCAA+AGG - chr7_2:15167368-15167387 Msa1050450:CDS 35.0%
GATTGTTAGTGATGCTAATG+CGG - chr7_2:15167456-15167475 Msa1050450:intron 35.0%
! TCTGAGATTAACACTAATCC+TGG - chr7_2:15167526-15167545 Msa1050450:CDS 35.0%
CCTTCTTGATTAACATAACC+AGG + chr7_2:15167547-15167566 None:intergenic 35.0%
CCTGGTTATGTTAATCAAGA+AGG - chr7_2:15167544-15167563 Msa1050450:CDS 35.0%
! GCTTTGTTGAAGTCATGATA+AGG + chr7_2:15167586-15167605 None:intergenic 35.0%
TTATACCTTGCACATGTTTG+GGG + chr7_2:15167632-15167651 None:intergenic 35.0%
GATTATACCTTGCACATGTT+TGG + chr7_2:15167634-15167653 None:intergenic 35.0%
AACAAGAGGTTTATCTACCT+TGG - chr7_2:15167776-15167795 Msa1050450:CDS 35.0%
! AAGGAAGAATTTTCCAGTGT+GGG - chr7_2:15167859-15167878 Msa1050450:intron 35.0%
ACGCCATGGAAATTCTTTAT+AGG - chr7_2:15168040-15168059 Msa1050450:five_prime_UTR 35.0%
CGCCATGGAAATTCTTTATA+GGG - chr7_2:15168041-15168060 Msa1050450:five_prime_UTR 35.0%
TCTCTCAGTGCTCTCAAAAT+TGG + chr7_2:15166950-15166969 None:intergenic 40.0%
TTTGAGTGGTGAGAAAGTGA+AGG + chr7_2:15167036-15167055 None:intergenic 40.0%
! AATTGAAAGGCATAGTGGGA+AGG + chr7_2:15167172-15167191 None:intergenic 40.0%
AACCATCGAAGAGATTGAAG+AGG - chr7_2:15167357-15167376 Msa1050450:CDS 40.0%
AACCTCTTCAATCTCTTCGA+TGG + chr7_2:15167362-15167381 None:intergenic 40.0%
ATGCATTAGGGTGTGATTTG+AGG - chr7_2:15167434-15167453 Msa1050450:intron 40.0%
GACTTCAACAAAGCTTCTCA+TGG - chr7_2:15167592-15167611 Msa1050450:CDS 40.0%
TATACCTTGCACATGTTTGG+GGG + chr7_2:15167631-15167650 None:intergenic 40.0%
AGGTTTATCTACCTTGGTGA+TGG - chr7_2:15167782-15167801 Msa1050450:CDS 40.0%
CTTCTCTAAGTCTCAAGCTA+GGG + chr7_2:15167823-15167842 None:intergenic 40.0%
CACACTGGAAAATTCTTCCT+TGG + chr7_2:15167860-15167879 None:intergenic 40.0%
! CAAGGAAGAATTTTCCAGTG+TGG - chr7_2:15167858-15167877 Msa1050450:intron 40.0%
TTGCATATCAAATCCCACAC+TGG + chr7_2:15167875-15167894 None:intergenic 40.0%
AGTGTGGGATTTGATATGCA+AGG - chr7_2:15167874-15167893 Msa1050450:intron 40.0%
ATTTCCATGGCGTTCCTTAA+AGG + chr7_2:15168033-15168052 None:intergenic 40.0%
TCTCTCAGTGCTCTCAAAAT+TGG + chr7_2:15166950-15166969 None:intergenic 40.0%
TTTGAGTGGTGAGAAAGTGA+AGG + chr7_2:15167036-15167055 None:intergenic 40.0%
! AATTGAAAGGCATAGTGGGA+AGG + chr7_2:15167172-15167191 None:intergenic 40.0%
AACCATCGAAGAGATTGAAG+AGG - chr7_2:15167357-15167376 Msa1050450:CDS 40.0%
AACCTCTTCAATCTCTTCGA+TGG + chr7_2:15167362-15167381 None:intergenic 40.0%
ATGCATTAGGGTGTGATTTG+AGG - chr7_2:15167434-15167453 Msa1050450:intron 40.0%
GACTTCAACAAAGCTTCTCA+TGG - chr7_2:15167592-15167611 Msa1050450:CDS 40.0%
TATACCTTGCACATGTTTGG+GGG + chr7_2:15167631-15167650 None:intergenic 40.0%
AGGTTTATCTACCTTGGTGA+TGG - chr7_2:15167782-15167801 Msa1050450:CDS 40.0%
CTTCTCTAAGTCTCAAGCTA+GGG + chr7_2:15167823-15167842 None:intergenic 40.0%
CACACTGGAAAATTCTTCCT+TGG + chr7_2:15167860-15167879 None:intergenic 40.0%
! CAAGGAAGAATTTTCCAGTG+TGG - chr7_2:15167858-15167877 Msa1050450:intron 40.0%
TTGCATATCAAATCCCACAC+TGG + chr7_2:15167875-15167894 None:intergenic 40.0%
AGTGTGGGATTTGATATGCA+AGG - chr7_2:15167874-15167893 Msa1050450:intron 40.0%
ATTTCCATGGCGTTCCTTAA+AGG + chr7_2:15168033-15168052 None:intergenic 40.0%
CCTTCTCTAAGTCTCAAGCT+AGG + chr7_2:15167824-15167843 None:intergenic 45.0%
CCTAGCTTGAGACTTAGAGA+AGG - chr7_2:15167821-15167840 Msa1050450:intron 45.0%
AAGGTGACTTTGTGATGCCA+AGG - chr7_2:15167840-15167859 Msa1050450:intron 45.0%
! TGATCCTTTAAGGAACGCCA+TGG - chr7_2:15168026-15168045 Msa1050450:five_prime_UTR 45.0%
CCTTCTCTAAGTCTCAAGCT+AGG + chr7_2:15167824-15167843 None:intergenic 45.0%
CCTAGCTTGAGACTTAGAGA+AGG - chr7_2:15167821-15167840 Msa1050450:intron 45.0%
AAGGTGACTTTGTGATGCCA+AGG - chr7_2:15167840-15167859 Msa1050450:intron 45.0%
! TGATCCTTTAAGGAACGCCA+TGG - chr7_2:15168026-15168045 Msa1050450:five_prime_UTR 45.0%
!! TAAATTATTATTATTATTTC+AGG - chr7_2:15167718-15167737 Msa1050450:CDS 5.0%
!! AAATTATTATTATTATTTCA+GGG - chr7_2:15167719-15167738 Msa1050450:CDS 5.0%
!! TAAATTATTATTATTATTTC+AGG - chr7_2:15167718-15167737 Msa1050450:CDS 5.0%
!! AAATTATTATTATTATTTCA+GGG - chr7_2:15167719-15167738 Msa1050450:CDS 5.0%
TTGTCCCCCAAACATGTGCA+AGG - chr7_2:15167624-15167643 Msa1050450:CDS 50.0%
!! ATCTACCTTGGTGATGGTGC+TGG - chr7_2:15167788-15167807 Msa1050450:CDS 50.0%
!! TCTACCTTGGTGATGGTGCT+GGG - chr7_2:15167789-15167808 Msa1050450:CDS 50.0%
TTGTCCCCCAAACATGTGCA+AGG - chr7_2:15167624-15167643 Msa1050450:CDS 50.0%
!! ATCTACCTTGGTGATGGTGC+TGG - chr7_2:15167788-15167807 Msa1050450:CDS 50.0%
!! TCTACCTTGGTGATGGTGCT+GGG - chr7_2:15167789-15167808 Msa1050450:CDS 50.0%
!! CTACCTTGGTGATGGTGCTG+GGG - chr7_2:15167790-15167809 Msa1050450:CDS 55.0%
!! CTACCTTGGTGATGGTGCTG+GGG - chr7_2:15167790-15167809 Msa1050450:CDS 55.0%
AGTCCCCAGCACCATCACCA+AGG + chr7_2:15167796-15167815 None:intergenic 60.0%
AGTCCCCAGCACCATCACCA+AGG + chr7_2:15167796-15167815 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr7_2 gene 15166936 15168204 15166936 ID=Msa1050450;Name=Msa1050450
chr7_2 mRNA 15166936 15168204 15166936 ID=Msa1050450-mRNA-1;Parent=Msa1050450;Name=Msa1050450-mRNA-1;_AED=0.04;_eAED=0.04;_QI=243|1|1|1|1|1|3|297|170
chr7_2 exon 15166936 15167400 15166936 ID=Msa1050450-mRNA-1:exon:3632;Parent=Msa1050450-mRNA-1
chr7_2 exon 15167495 15167818 15167495 ID=Msa1050450-mRNA-1:exon:3631;Parent=Msa1050450-mRNA-1
chr7_2 exon 15167941 15168204 15167941 ID=Msa1050450-mRNA-1:exon:3630;Parent=Msa1050450-mRNA-1
chr7_2 five_prime_UTR 15167962 15168204 15167962 ID=Msa1050450-mRNA-1:five_prime_utr;Parent=Msa1050450-mRNA-1
chr7_2 CDS 15167941 15167961 15167941 ID=Msa1050450-mRNA-1:cds;Parent=Msa1050450-mRNA-1
chr7_2 CDS 15167495 15167818 15167495 ID=Msa1050450-mRNA-1:cds;Parent=Msa1050450-mRNA-1
chr7_2 CDS 15167233 15167400 15167233 ID=Msa1050450-mRNA-1:cds;Parent=Msa1050450-mRNA-1
chr7_2 three_prime_UTR 15166936 15167232 15166936 ID=Msa1050450-mRNA-1:three_prime_utr;Parent=Msa1050450-mRNA-1
Gene Sequence

>Msa1050450

ATGCCTTTCAATTCTCTTATGGACAGAATGATGATGGAGCTTCATTCAAATGGGAAAACCATCGAAGAGATTGAAGAGGTTCTTCAAAGGATTCCAATTAATCACAGAATAATATCTGCTATTAAATCAGCTTATGCATTAGGGTGTGATTTGAGGATTGTTAGTGATGCTAATGCGGTTTTCATCAACACAATTTTGAAGCATTTGGGAATAAGTGAATGCTTTTCTGAGATTAACACTAATCCTGGTTATGTTAATCAAGAAGGAAGATTAAAAGTTATGCCTTATCATGACTTCAACAAAGCTTCTCATGGTTGCACTCTTTGTCCCCCAAACATGTGCAAGGGTTTAATCATTGATAGAATCCAAAAATCAATTTCGAACAAGAGGTTTATCTACCTTGGTGATGGTGCTGGGGACTATTGCCCTAGCTTGAGACTTAGAGAAGGTGACTTTGTGATGCCAAGGAAGAATTTTCCAGTGTGGGATTTGATATGCAAGGAAAAATTCTAA
Protein sequence

>Msa1050450

MPFNSLMDRMMMELHSNGKTIEEIEEVLQRIPINHRIISAIKSAYALGCDLRIVSDANAVFINTILKHLGISECFSEINTNPGYVNQEGRLKVMPYHDFNKASHGCTLCPPNMCKGLIIDRIQKSISNKRFIYLGDGAGDYCPSLRLREGDFVMPRKNFPVWDLICKEKF*