AlfalfaGEDB | Msa1067540

M. sativa cultivar zhongmu-4 / Msa1067540

KEGG: K09422
GO: -
PFAM: PF00479.25

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1067540	XP_013469134.1	95.930	172	5	2	328	497	30	201	1.40e-107	338

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1067540	sp\|O24357\|G6PDC_SPIOL	85.124	121	14	1	45	161	228	348	1.56e-59	211

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1067540	A0A072VMV6	95.930	172	5	2	328	497	30	201	6.69e-108	338

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TFs/TRs:

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Gene ID	Type	Classification
Msa1067540	TF	MYB

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0270360	Msa1067540	0.982924	2.315210e-156	-8.615850e-47
Msa0293450	Msa1067540	0.855015	8.377605e-62	-8.615850e-47
Msa0801540	Msa1067540	0.962850	2.264073e-121	-8.615850e-47
Msa0810830	Msa1067540	0.984942	4.729442e-162	-8.615850e-47
Msa1050020	Msa1067540	0.812225	4.717619e-51	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1067540	MtrunA17_Chr1g0193801	95.930	172	5	2	328	497	30	201	1.29e-111	338
Msa1067540	MtrunA17_Chr7g0270891	64.593	209	59	4	328	532	30	227	2.33e-82	262
Msa1067540	MtrunA17_Chr1g0193371	75.362	138	29	3	328	462	30	165	2.08e-63	212
Msa1067540	MtrunA17_Chr7g0222441	66.471	170	44	3	45	201	250	419	3.88e-62	215
Msa1067540	MtrunA17_Chr8g0358431	80.992	121	19	1	45	161	27	147	1.89e-60	199
Msa1067540	MtrunA17_Chr8g0341321	83.471	121	16	1	45	161	237	357	3.97e-59	206
Msa1067540	MtrunA17_Chr8g0356771	71.852	135	20	2	45	161	27	161	1.70e-56	187
Msa1067540	MtrunA17_Chr4g0058631	79.612	103	20	1	328	429	30	132	1.43e-53	186
Msa1067540	MtrunA17_Chr1g0211161	65.041	123	38	2	328	449	31	149	6.93e-50	172
Msa1067540	MtrunA17_Chr7g0271241	79.091	110	22	1	328	436	30	139	2.12e-49	175

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1067540	AT4G01680.3	78.049	123	25	2	328	449	30	151	1.59e-61	203
Msa1067540	AT4G01680.1	78.049	123	25	2	328	449	30	151	2.89e-60	203
Msa1067540	AT5G13110.1	84.298	121	15	1	45	161	251	371	4.44e-60	210
Msa1067540	AT1G24280.1	84.298	121	15	1	45	161	254	374	5.47e-60	209
Msa1067540	AT1G57560.1	78.448	116	22	2	328	440	30	145	6.14e-58	196
Msa1067540	AT5G35790.1	81.818	121	18	1	45	161	232	352	3.13e-57	202
Msa1067540	AT1G57560.2	83.654	104	16	1	328	430	30	133	5.01e-57	192
Msa1067540	AT4G01680.2	71.111	135	25	3	328	449	30	163	6.76e-57	195
Msa1067540	AT1G09540.1	83.654	104	16	1	328	430	30	133	9.57e-56	192
Msa1067540	AT5G26660.1	83.000	100	16	1	328	426	30	129	3.51e-54	187

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Find 158 sgRNAs with CRISPR-Local

Find 444 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGCATATGCAATGGAAA+TGG	0.406668	7_2:+62283022	Msa1067540:CDS
AAAAGGGAACGTGTGATTCA+AGG	0.489998	7_2:+62285479	Msa1067540:three_prime_UTR
AAAGATGTGGTAAGAGCTGC+AGG	0.517924	7_2:+62282465	Msa1067540:CDS
AACAATATTTATTCTGCAGG+TGG	0.566902	7_2:-62282278	None:intergenic
AACTGTTTGATGCTGATGAA+TGG	0.424478	7_2:-62285209	Msa1067550:CDS
AACTTGATGTTTAATCCACC+AGG	0.497562	7_2:+62285054	Msa1067540:intron
AAGAACAGAATCCAAAGAAA+TGG	0.369003	7_2:+62285444	Msa1067540:three_prime_UTR
AAGAAGAGACTGAGGCAAAA+AGG	0.381753	7_2:+62282816	Msa1067540:CDS
AAGAAGCCATCAAACAGTTG+AGG	0.607756	7_2:+62284183	Msa1067540:three_prime_UTR
AAGAGGAACATTCTCACAAG+AGG	0.608650	7_2:+62282523	Msa1067540:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AGAGTAGAAGAAATGGAATT+AGG	+	chr7_2:62277729-62277748	Msa1067540:CDS	30.0%
GAGTAGAAGAAATGGAATTA+GGG	+	chr7_2:62277730-62277749	Msa1067540:CDS	30.0%
CCTTTAATTCTTTATGGTGA+TGG	-	chr7_2:62277863-62277882	None:intergenic	30.0%
CCATCACCATAAAGAATTAA+AGG	+	chr7_2:62277860-62277879	Msa1067540:intron	30.0%
TCACCATAAAGAATTAAAGG+TGG	+	chr7_2:62277863-62277882	Msa1067540:intron	30.0%
CACCATAAAGAATTAAAGGT+GGG	+	chr7_2:62277864-62277883	Msa1067540:intron	30.0%
AACCCACCTTTAATTCTTTA+TGG	-	chr7_2:62277869-62277888	None:intergenic	30.0%
TGAATGATAGCATGATTGAA+AGG	+	chr7_2:62278054-62278073	Msa1067540:intron	30.0%
GGAGATTATAAAAATGAGCT+AGG	+	chr7_2:62278152-62278171	Msa1067540:intron	30.0%
TCACATGAAGACAATGAATT+TGG	-	chr7_2:62278437-62278456	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr7_2	gene	62277677	62285655	62277677	ID=Msa1067540;Name=Msa1067540
chr7_2	mRNA	62277677	62285655	62277677	ID=Msa1067540-mRNA-1;Parent=Msa1067540;Name=Msa1067540-mRNA-1;_AED=0.28;_eAED=0.30;_QI=0\|0.55\|0.52\|1\|0.83\|0.78\|19\|987\|570
chr7_2	exon	62277677	62277768	62277677	ID=Msa1067540-mRNA-1:exon:12569;Parent=Msa1067540-mRNA-1
chr7_2	exon	62278832	62278896	62278832	ID=Msa1067540-mRNA-1:exon:12570;Parent=Msa1067540-mRNA-1
chr7_2	exon	62279245	62279296	62279245	ID=Msa1067540-mRNA-1:exon:12571;Parent=Msa1067540-mRNA-1
chr7_2	exon	62279649	62279798	62279649	ID=Msa1067540-mRNA-1:exon:12572;Parent=Msa1067540-mRNA-1
chr7_2	exon	62279886	62280016	62279886	ID=Msa1067540-mRNA-1:exon:12573;Parent=Msa1067540-mRNA-1
chr7_2	exon	62280706	62280783	62280706	ID=Msa1067540-mRNA-1:exon:12574;Parent=Msa1067540-mRNA-1
chr7_2	exon	62281092	62281212	62281092	ID=Msa1067540-mRNA-1:exon:12575;Parent=Msa1067540-mRNA-1
chr7_2	exon	62281615	62281739	62281615	ID=Msa1067540-mRNA-1:exon:12576;Parent=Msa1067540-mRNA-1
chr7_2	exon	62281839	62281891	62281839	ID=Msa1067540-mRNA-1:exon:12577;Parent=Msa1067540-mRNA-1
chr7_2	exon	62282104	62282184	62282104	ID=Msa1067540-mRNA-1:exon:12578;Parent=Msa1067540-mRNA-1
chr7_2	exon	62282277	62282355	62282277	ID=Msa1067540-mRNA-1:exon:12579;Parent=Msa1067540-mRNA-1
chr7_2	exon	62282459	62282588	62282459	ID=Msa1067540-mRNA-1:exon:12580;Parent=Msa1067540-mRNA-1
chr7_2	exon	62282746	62283078	62282746	ID=Msa1067540-mRNA-1:exon:12581;Parent=Msa1067540-mRNA-1
chr7_2	exon	62283566	62283697	62283566	ID=Msa1067540-mRNA-1:exon:12582;Parent=Msa1067540-mRNA-1
chr7_2	exon	62283850	62283910	62283850	ID=Msa1067540-mRNA-1:exon:12583;Parent=Msa1067540-mRNA-1
chr7_2	exon	62283996	62284046	62283996	ID=Msa1067540-mRNA-1:exon:12584;Parent=Msa1067540-mRNA-1
chr7_2	exon	62284173	62284351	62284173	ID=Msa1067540-mRNA-1:exon:12585;Parent=Msa1067540-mRNA-1
chr7_2	exon	62284519	62284709	62284519	ID=Msa1067540-mRNA-1:exon:12586;Parent=Msa1067540-mRNA-1
chr7_2	exon	62285060	62285655	62285060	ID=Msa1067540-mRNA-1:exon:12587;Parent=Msa1067540-mRNA-1
chr7_2	CDS	62277677	62277768	62277677	ID=Msa1067540-mRNA-1:cds;Parent=Msa1067540-mRNA-1
chr7_2	CDS	62278832	62278896	62278832	ID=Msa1067540-mRNA-1:cds;Parent=Msa1067540-mRNA-1
chr7_2	CDS	62279245	62279296	62279245	ID=Msa1067540-mRNA-1:cds;Parent=Msa1067540-mRNA-1
chr7_2	CDS	62279649	62279798	62279649	ID=Msa1067540-mRNA-1:cds;Parent=Msa1067540-mRNA-1
chr7_2	CDS	62279886	62280016	62279886	ID=Msa1067540-mRNA-1:cds;Parent=Msa1067540-mRNA-1
chr7_2	CDS	62280706	62280783	62280706	ID=Msa1067540-mRNA-1:cds;Parent=Msa1067540-mRNA-1
chr7_2	CDS	62281092	62281212	62281092	ID=Msa1067540-mRNA-1:cds;Parent=Msa1067540-mRNA-1
chr7_2	CDS	62281615	62281739	62281615	ID=Msa1067540-mRNA-1:cds;Parent=Msa1067540-mRNA-1
chr7_2	CDS	62281839	62281891	62281839	ID=Msa1067540-mRNA-1:cds;Parent=Msa1067540-mRNA-1
chr7_2	CDS	62282104	62282184	62282104	ID=Msa1067540-mRNA-1:cds;Parent=Msa1067540-mRNA-1
chr7_2	CDS	62282277	62282355	62282277	ID=Msa1067540-mRNA-1:cds;Parent=Msa1067540-mRNA-1
chr7_2	CDS	62282459	62282588	62282459	ID=Msa1067540-mRNA-1:cds;Parent=Msa1067540-mRNA-1
chr7_2	CDS	62282746	62283078	62282746	ID=Msa1067540-mRNA-1:cds;Parent=Msa1067540-mRNA-1
chr7_2	CDS	62283566	62283697	62283566	ID=Msa1067540-mRNA-1:cds;Parent=Msa1067540-mRNA-1
chr7_2	CDS	62283850	62283910	62283850	ID=Msa1067540-mRNA-1:cds;Parent=Msa1067540-mRNA-1
chr7_2	CDS	62283996	62284025	62283996	ID=Msa1067540-mRNA-1:cds;Parent=Msa1067540-mRNA-1
chr7_2	three_prime_UTR	62284026	62284046	62284026	ID=Msa1067540-mRNA-1:three_prime_utr;Parent=Msa1067540-mRNA-1
chr7_2	three_prime_UTR	62284173	62284351	62284173	ID=Msa1067540-mRNA-1:three_prime_utr;Parent=Msa1067540-mRNA-1
chr7_2	three_prime_UTR	62284519	62284709	62284519	ID=Msa1067540-mRNA-1:three_prime_utr;Parent=Msa1067540-mRNA-1
chr7_2	three_prime_UTR	62285060	62285655	62285060	ID=Msa1067540-mRNA-1:three_prime_utr;Parent=Msa1067540-mRNA-1

Gene Sequence

>Msa1067540

ATGTTAAGTTTGGGTAAGAGGGGTCTGAGGTCGAGAGTGAGGCAGAGGGAGAAGAGTAGAAGAAATGGAATTAGGGTTCTGAGAAAGAAGAAGGATGTTGGCAGTAATATCGCATTGAAAATTCTGAAGCGTGTAGAAAAAGGTTGGGGTCAAGGAACTGCTTTAACAAGATCATTCAAGCAGTATCTGACTGAGGATCAAATTTTCAGGATCGATCACTATCTTGGAAAAGAGCTTGTGGAAAATCTTTTTGTTCTCCGATTCTCAAATCTAATTTTTGAACCTTTGTGGTCGAGGAAATACATAAAAAATGTACAGTTGATATTCTCAGAAGATTTTGGTACTGAAGGACGTGGCAGGTACTTTGACAATTATGGTATAATTAGACATATTATGCAGAATCATCTACTTCAAATACTAGCCCTCTTTGCAATGGAAACCCCTGTTAGTTTGGATGTAGAAGACATTAGAAATGAAAAGGTCTCATATGTAATTGATATTACAAGGATGGTAGTTCTTCACCCAAACGGGGCTATTGCACTTCAGAAGCATTTTGAATCTGAAAAAGAAAAGATAGTTGCATCTCAACCTGCCTTCTCCGGTGAAGGTGATTCCTGGACAACAAATGGACTTTTGGAGCAGCTCAGTCAGACTTGGGATAAGTCAGATTATTTGTGTTACATGACAGAAAATTTATACGTGCAGATCTTTGATCTATTTGATGTCAAGAGAAAGGGTGTTGTTGATTTTGATGACTTCGTTAGATCTCTCAATGTCTTCCACCCGAACGCAGCGCTAGAAGACAATATAGAATTCTCATTTAGGCTCTATGATTTGCACATTACTGGCTTTATAGAGCGTCCAGAGGTCAAGCAAATGTTAATTGCACTTCTTTTCGAGTTTGAAATGAAGTTGGCGGATGATGTGATAGAAACAATTCTAGACAAGTCCACCTGCAGAATAAATATTGTTGCTGATAGAACAACGTATGGTCATGGATGTTGGAGTTCAGTACCTAAGCAAGCAGGTTTGCAAAGATGTGGTAAGAGCTGCAGGCTTAGGTGGATCAATTACTTAAGACCTGATTTAAAAAGAGGAACATTCTCACAAGAGGAAGAAAATCACATTGTTGAACTTCATGCAGTACTAGGAAATAGATGGTCTCAAATTGCGGCGCAATTGCCGGGAAGGACAGACAATGAAATGAATCTATGGAACTCATGTTTGAAGAAGAGACTGAGGCAAAAAGGTATAGATCCTGTGACTCATAAACCATTGTCTGAGGTTGAGAATAATGGAGAGGAAAATGCTAATAAGAGTCAAGAGAAAGCACCAGAGATATTATTATCCAATGAATTGAACCTTTTGAAATCAGAGAGTTCAAAATCAGATGCAGCTTCTAGCTATGAGCAAAGAACATCTTCAATTTCACCAAAAGCATATGCAATGGAAATGGAAGGTTCTTGCAACAAAGAGTTTTTCATAGATAGAGTTAGTTTCGAAACTTTTGTTTTGACTGTTTGTGCCTTGCAGTTGAAGTCTCCTTCAGTTTCAAGGAAACCATTGAAACCTGATAACAAGAAACATTTTAACGAGGACGATTCATGTTCAGTTGCTTCTGGGGCGACTGTTGCTTCTGCTCCAAGCTTTTGGTCAGCTGAACGTGCACAAAGAAGAAAGGAGTTCTATTCAAAATCAAGCAATGGAAGCTGA

Protein sequence

>Msa1067540

MLSLGKRGLRSRVRQREKSRRNGIRVLRKKKDVGSNIALKILKRVEKGWGQGTALTRSFKQYLTEDQIFRIDHYLGKELVENLFVLRFSNLIFEPLWSRKYIKNVQLIFSEDFGTEGRGRYFDNYGIIRHIMQNHLLQILALFAMETPVSLDVEDIRNEKVSYVIDITRMVVLHPNGAIALQKHFESEKEKIVASQPAFSGEGDSWTTNGLLEQLSQTWDKSDYLCYMTENLYVQIFDLFDVKRKGVVDFDDFVRSLNVFHPNAALEDNIEFSFRLYDLHITGFIERPEVKQMLIALLFEFEMKLADDVIETILDKSTCRINIVADRTTYGHGCWSSVPKQAGLQRCGKSCRLRWINYLRPDLKRGTFSQEEENHIVELHAVLGNRWSQIAAQLPGRTDNEMNLWNSCLKKRLRQKGIDPVTHKPLSEVENNGEENANKSQEKAPEILLSNELNLLKSESSKSDAASSYEQRTSSISPKAYAMEMEGSCNKEFFIDRVSFETFVLTVCALQLKSPSVSRKPLKPDNKKHFNEDDSCSVASGATVASAPSFWSAERAQRRKEFYSKSSNGS*