AlfalfaGEDB | Msa1073570

M. sativa cultivar zhongmu-4 / Msa1073570

KEGG: K14508|K21407
GO: GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005783 GO:0012505 GO:0016020 GO:0016787 GO:0016788 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1073570	AES81260.1	96.907	97	3	0	4	100	102	198	7.59e-61	200

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1073570	sp\|Q9SLG8\|SSL2_ARATH	58.242	91	36	2	10	100	94	182	2.08e-30	113

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1073570	G7KRP1	96.907	97	3	0	4	100	102	198	3.62e-61	200

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0795930	Msa1073570	0.814384	1.583955e-51	-8.615850e-47
Msa0149600	Msa1073570	0.803372	3.597188e-49	-8.615850e-47
Msa0393470	Msa1073570	0.816940	4.267622e-52	-8.615850e-47
Msa1033710	Msa1073570	0.853782	1.902966e-61	-8.615850e-47
Msa1073570	Msa1279870	0.809215	2.112079e-50	-8.615850e-47
Msa1073570	Msa1287690	0.805704	1.173634e-49	-8.615850e-47
Msa1073570	Msa1287710	0.815352	9.658742e-52	-8.615850e-47
Msa0580430	Msa1073570	0.813623	2.330471e-51	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1073570	MtrunA17_Chr7g0256601	96.907	97	3	0	4	100	132	228	1.05e-64	201
Msa1073570	MtrunA17_Chr7g0256591	93.684	95	6	0	6	100	103	197	1.10e-60	189
Msa1073570	MtrunA17_Chr7g0256571	51.685	89	40	2	12	100	98	183	4.25e-26	99.0
Msa1073570	MtrunA17_Chr7g0256561	52.174	92	41	2	8	99	98	186	7.76e-26	97.8
Msa1073570	MtrunA17_Chr7g0256581	51.111	90	41	2	11	100	97	183	3.15e-25	96.7
Msa1073570	MtrunA17_Chr5g0443561	47.778	90	44	2	11	100	92	178	3.09e-23	90.9
Msa1073570	MtrunA17_Chr4g0025341	56.250	80	33	2	21	100	126	203	7.51e-23	90.5
Msa1073570	MtrunA17_Chr2g0329531	49.367	79	38	2	21	99	142	218	1.77e-21	87.0
Msa1073570	MtrunA17_Chr2g0307721	40.000	95	56	1	6	100	88	181	8.20e-21	85.5
Msa1073570	MtrunA17_Chr2g0307721	36.697	109	51	3	9	100	525	632	5.01e-16	72.0
Msa1073570	MtrunA17_Chr2g0307721	39.744	78	46	1	23	100	345	421	3.96e-14	66.6
Msa1073570	MtrunA17_Chr2g0322241	55.128	78	33	2	23	100	132	207	2.27e-20	84.0
Msa1073570	MtrunA17_Chr4g0062031	43.678	87	47	2	12	98	85	169	6.06e-18	76.6
Msa1073570	MtrunA17_Chr4g0062001	47.945	73	37	1	26	98	99	170	7.02e-17	73.9
Msa1073570	MtrunA17_Chr4g0062021	46.053	76	40	1	23	98	108	182	2.38e-16	72.4
Msa1073570	MtrunA17_Chr8g0376581	46.575	73	38	1	26	98	97	168	3.69e-16	71.6
Msa1073570	MtrunA17_Chr4g0062051	36.735	98	60	2	3	100	80	175	3.15e-15	67.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1073570	AT2G41290.1	58.242	91	36	2	10	100	94	182	2.11e-31	113
Msa1073570	AT3G57030.1	49.451	91	43	2	10	100	99	186	7.30e-29	107
Msa1073570	AT5G22020.3	56.250	80	33	2	21	100	130	207	4.49e-22	89.0
Msa1073570	AT5G22020.1	56.250	80	33	2	21	100	130	207	4.49e-22	89.0
Msa1073570	AT5G22020.2	56.250	80	33	2	21	100	130	207	4.49e-22	89.0
Msa1073570	AT3G59530.3	50.633	79	37	2	21	99	144	220	2.59e-21	86.7
Msa1073570	AT3G59530.2	50.633	79	37	2	21	99	144	220	2.59e-21	86.7
Msa1073570	AT3G59530.1	50.633	79	37	2	21	99	144	220	2.59e-21	86.7
Msa1073570	AT1G08470.1	53.012	83	37	2	18	100	121	201	7.35e-21	85.5
Msa1073570	AT1G74020.1	54.167	72	32	1	26	97	96	166	1.74e-19	81.3
Msa1073570	AT1G74010.1	52.000	75	35	1	26	100	94	167	3.44e-19	80.5
Msa1073570	AT1G74000.1	52.000	75	35	1	26	100	98	171	6.78e-18	77.0
Msa1073570	AT2G41300.1	48.052	77	39	1	21	97	127	202	1.40e-17	76.3
Msa1073570	AT3G57020.1	44.000	75	41	1	26	100	107	180	2.90e-15	69.7
Msa1073570	AT3G57020.2	42.466	73	41	1	28	100	95	166	7.10e-14	65.9
Msa1073570	AT3G57010.1	42.667	75	42	1	26	100	108	181	1.45e-13	65.1

Find 26 sgRNAs with CRISPR-Local

Find 33 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CCACATATGTGTTCTTTCTT+TGG	0.231810	7_2:-71143033	None:intergenic
TTCTTTGGATGCTCCTTGTA+TGG	0.320582	7_2:-71143018	None:intergenic
TTTGGCTTTCACAAACAGTT+TGG	0.368869	7_2:+71143191	Msa1073570:CDS
CACATATGTGGGCGCCCACT+TGG	0.369191	7_2:+71143045	Msa1073570:CDS
GTCATATCACATGCAGTAGA+AGG	0.445885	7_2:+71143162	Msa1073570:CDS
TTGGATATTGATCAACGAAC+TGG	0.446101	7_2:+71143210	Msa1073570:CDS
TCTCTCATATTTAGAACTGC+TGG	0.447647	7_2:-71143250	None:intergenic
TGCAGTAGAAGGTCAACCTT+TGG	0.453896	7_2:+71143173	Msa1073570:CDS
TTGTGCTTCAACGTAGCATC+TGG	0.463806	7_2:+71143069	Msa1073570:CDS
TTATGTTGCTGATGCATACA+TGG	0.494983	7_2:+71143098	Msa1073570:CDS
CTAGTTGTTATTGAGCCCAC+TGG	0.517702	7_2:+71143123	Msa1073570:CDS
ACATATGTGGGCGCCCACTT+GGG	0.533191	7_2:+71143046	Msa1073570:CDS
CCAAAGAAAGAACACATATG+TGG	0.541865	7_2:+71143033	Msa1073570:CDS
ATGCATCAGCAACATAAAGT+TGG	0.561864	7_2:-71143092	None:intergenic
AGAGTGTGGAGGACCATACA+AGG	0.571122	7_2:+71143005	Msa1073570:CDS
AAACTGTTTGTGAAAGCCAA+AGG	0.587101	7_2:-71143189	None:intergenic
GCAGTTCTAAATATGAGAGA+AGG	0.590598	7_2:+71143253	Msa1073570:CDS
TTTCTAGGAACGCCACCAGT+GGG	0.600789	7_2:-71143138	None:intergenic
GATGATGATGATGAAGAGTG+TGG	0.601295	7_2:+71142991	Msa1073570:CDS
CAAAGAAAGAACACATATGT+GGG	0.603235	7_2:+71143034	Msa1073570:CDS
CTCATATTTAGAACTGCTGG+AGG	0.621135	7_2:-71143247	None:intergenic
GTTGTTATTGAGCCCACTGG+TGG	0.630574	7_2:+71143126	Msa1073570:CDS
CGTTGAAGCACAACCCAAGT+GGG	0.630882	7_2:-71143059	None:intergenic
TATGTTGCTGATGCATACAT+GGG	0.661782	7_2:+71143099	Msa1073570:CDS
ACGTTGAAGCACAACCCAAG+TGG	0.670983	7_2:-71143060	None:intergenic
GATGATGATGAAGAGTGTGG+AGG	0.746405	7_2:+71142994	Msa1073570:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AATAAGATGAGATGAACAAT+TGG	-	chr7_2:71142929-71142948	None:intergenic	25.0%
!!!	TAAAGTTGATATGGTTGATT+TGG	+	chr7_2:71142958-71142977	Msa1073570:intron	25.0%
	TTCATCTCATCTTATTGTTG+TGG	+	chr7_2:71142933-71142952	Msa1073570:intron	30.0%
!!	GTTGTGGAATAAAGTTGATA+TGG	+	chr7_2:71142949-71142968	Msa1073570:intron	30.0%
	CAAAGAAAGAACACATATGT+GGG	+	chr7_2:71143034-71143053	Msa1073570:CDS	30.0%
	CCACATATGTGTTCTTTCTT+TGG	-	chr7_2:71143036-71143055	None:intergenic	35.0%
	CCAAAGAAAGAACACATATG+TGG	+	chr7_2:71143033-71143052	Msa1073570:CDS	35.0%
	ATGCATCAGCAACATAAAGT+TGG	-	chr7_2:71143095-71143114	None:intergenic	35.0%
	TTATGTTGCTGATGCATACA+TGG	+	chr7_2:71143098-71143117	Msa1073570:CDS	35.0%
	TATGTTGCTGATGCATACAT+GGG	+	chr7_2:71143099-71143118	Msa1073570:CDS	35.0%
!	GCATGTGATATGACTTTTCT+AGG	-	chr7_2:71143156-71143175	None:intergenic	35.0%
	AAACTGTTTGTGAAAGCCAA+AGG	-	chr7_2:71143192-71143211	None:intergenic	35.0%
!!	TTTGGCTTTCACAAACAGTT+TGG	+	chr7_2:71143191-71143210	Msa1073570:CDS	35.0%
	TTGGATATTGATCAACGAAC+TGG	+	chr7_2:71143210-71143229	Msa1073570:CDS	35.0%
!	TCTCTCATATTTAGAACTGC+TGG	-	chr7_2:71143253-71143272	None:intergenic	35.0%
!!	GCAGTTCTAAATATGAGAGA+AGG	+	chr7_2:71143253-71143272	Msa1073570:CDS	35.0%
!!	TGATATGGTTGATTTGGCAG+AGG	+	chr7_2:71142964-71142983	Msa1073570:intron	40.0%
!	GATGATGATGATGAAGAGTG+TGG	+	chr7_2:71142991-71143010	Msa1073570:CDS	40.0%
	TTCTTTGGATGCTCCTTGTA+TGG	-	chr7_2:71143021-71143040	None:intergenic	40.0%
	GTCATATCACATGCAGTAGA+AGG	+	chr7_2:71143162-71143181	Msa1073570:CDS	40.0%
!	CTCATATTTAGAACTGCTGG+AGG	-	chr7_2:71143250-71143269	None:intergenic	40.0%
!	GATGATGATGAAGAGTGTGG+AGG	+	chr7_2:71142994-71143013	Msa1073570:CDS	45.0%
	TTGTGCTTCAACGTAGCATC+TGG	+	chr7_2:71143069-71143088	Msa1073570:CDS	45.0%
!	CTAGTTGTTATTGAGCCCAC+TGG	+	chr7_2:71143123-71143142	Msa1073570:CDS	45.0%
	TGCAGTAGAAGGTCAACCTT+TGG	+	chr7_2:71143173-71143192	Msa1073570:CDS	45.0%
	AGAGTGTGGAGGACCATACA+AGG	+	chr7_2:71143005-71143024	Msa1073570:CDS	50.0%
	CGTTGAAGCACAACCCAAGT+GGG	-	chr7_2:71143062-71143081	None:intergenic	50.0%
	ACGTTGAAGCACAACCCAAG+TGG	-	chr7_2:71143063-71143082	None:intergenic	50.0%
!	GTTGTTATTGAGCCCACTGG+TGG	+	chr7_2:71143126-71143145	Msa1073570:CDS	50.0%
!	TTTCTAGGAACGCCACCAGT+GGG	-	chr7_2:71143141-71143160	None:intergenic	50.0%
!!	TTTTCTAGGAACGCCACCAG+TGG	-	chr7_2:71143142-71143161	None:intergenic	50.0%
	ACATATGTGGGCGCCCACTT+GGG	+	chr7_2:71143046-71143065	Msa1073570:CDS	55.0%
	CACATATGTGGGCGCCCACT+TGG	+	chr7_2:71143045-71143064	Msa1073570:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr7_2	gene	71142883	71143278	71142883	ID=Msa1073570;Name=Msa1073570
chr7_2	mRNA	71142883	71143278	71142883	ID=Msa1073570-mRNA-1;Parent=Msa1073570;Name=Msa1073570-mRNA-1;_AED=0.49;_eAED=0.49;_QI=0\|0\|0\|0.5\|1\|1\|2\|0\|100
chr7_2	exon	71142883	71142890	71142883	ID=Msa1073570-mRNA-1:exon:16107;Parent=Msa1073570-mRNA-1
chr7_2	exon	71142984	71143278	71142984	ID=Msa1073570-mRNA-1:exon:16108;Parent=Msa1073570-mRNA-1
chr7_2	CDS	71142883	71142890	71142883	ID=Msa1073570-mRNA-1:cds;Parent=Msa1073570-mRNA-1
chr7_2	CDS	71142984	71143278	71142984	ID=Msa1073570-mRNA-1:cds;Parent=Msa1073570-mRNA-1

Gene Sequence	>Msa1073570 ATGGAAACAGGTGATGATGATGATGATGAAGAGTGTGGAGGACCATACAAGGAGCATCCAAAGAAAGAACACATATGTGGGCGCCCACTTGGGTTGTGCTTCAACGTAGCATCTGGCCAACTTTATGTTGCTGATGCATACATGGGACTAGTTGTTATTGAGCCCACTGGTGGCGTTCCTAGAAAAGTCATATCACATGCAGTAGAAGGTCAACCTTTGGCTTTCACAAACAGTTTGGATATTGATCAACGAACTGGAGCAGTGTACTTTACCTCCAGCAGTTCTAAATATGAGAGAAGGTAA
Protein sequence	>Msa1073570 METGDDDDDEECGGPYKEHPKKEHICGRPLGLCFNVASGQLYVADAYMGLVVIEPTGGVPRKVISHAVEGQPLAFTNSLDIDQRTGAVYFTSSSSKYERR*