AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1053680

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1053680 MtrunA17_Chr7g0214791 97.129 209 6 0 1 209 1 209 3.31e-145 410
Msa1053680 MtrunA17_Chr7g0214791 100.000 145 0 0 107 251 207 351 3.90e-99 293
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1053680 AT1G44960.1 67.299 211 67 1 30 240 26 234 1.71e-103 301

Find 69 sgRNAs with CRISPR-Local

Find 286 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAATTTCCGGTACTGAAATT+TGG 0.132573 7_2:+24431157 None:intergenic
TATCTGATAAACTAGGAATT+TGG 0.145494 7_2:-24436124 Msa1053680:CDS
GCTCTAAGAAATCTCAATTT+TGG 0.229017 7_2:-24431251 Msa1053680:CDS
AGTTCTTCAGGTTAGTATTC+AGG 0.245543 7_2:-24431539 Msa1053680:intron
ACTGGTGCTTCTCTTCTCTT+TGG 0.275215 7_2:-24436029 Msa1053680:CDS
ACTAGATAAAATACCAACTA+TGG 0.282343 7_2:+24431215 None:intergenic
AATACTAACAACAACAAAAT+TGG 0.287741 7_2:+24430950 None:intergenic
ATCTGATAAACTAGGAATTT+GGG 0.313710 7_2:-24436123 Msa1053680:CDS
GACTTGATCTTCATTTAATC+TGG 0.331073 7_2:-24430878 Msa1053680:three_prime_UTR
AATTTCCGGTACTGAAATTT+GGG 0.342524 7_2:+24431158 None:intergenic
CCTTAGCTGCTACTGAAGTT+AGG 0.357035 7_2:-24431380 Msa1053680:CDS
GCAGCTGTTGCTACAGCCTC+TGG 0.372165 7_2:-24431273 Msa1053680:CDS
ATAAAGAATCATTGATGAAA+TGG 0.374496 7_2:-24436157 Msa1053680:CDS
TACCGATCGAACTGTCTGTT+TGG 0.377737 7_2:-24431008 Msa1053680:three_prime_UTR
TCTTTGTCAGAAGAAATTTC+CGG 0.385161 7_2:+24431144 None:intergenic
GTGCTTCTCTTCTCTTTGGT+TGG 0.388199 7_2:-24436025 Msa1053680:CDS
CAGCAATGGACTGGGCTAGA+AGG 0.407462 7_2:-24431506 Msa1053680:CDS
ACCGATCGAACTGTCTGTTT+GGG 0.409473 7_2:-24431007 Msa1053680:three_prime_UTR
CCTTATCTTTCTCCATTGGC+AGG 0.426737 7_2:-24435956 Msa1053680:intron
TTTCTTCTTCCTACCATTGT+CGG 0.428961 7_2:-24431345 Msa1053680:CDS
GAATTAAATCAATAACATAA+TGG 0.432097 7_2:-24436270 Msa1053680:exon
GGAAGTTTGTTCTTCTTGCT+CGG 0.432228 7_2:-24431437 Msa1053680:CDS
GGCGGAGAAGCAATCGGAAT+CGG 0.440129 7_2:-24436185 Msa1053680:CDS
ACATCTATAGGAAGTCTTGC+TGG 0.441068 7_2:-24431297 Msa1053680:CDS
TAAGGTATAGTTAATGATAT+AGG 0.443398 7_2:+24431398 None:intergenic
ACATAATGGCGGGGTGGTGG+TGG 0.456256 7_2:-24436256 Msa1053680:exon
GCCTTACTACTCCGATCATA+TGG 0.458022 7_2:-24436206 Msa1053680:CDS
TAAATCAATAACATAATGGC+GGG 0.458116 7_2:-24436266 Msa1053680:exon
GCTTCCTTATCTTTCTCCAT+TGG 0.464011 7_2:-24435960 Msa1053680:CDS
ATCTTACAGAATACATCTAT+AGG 0.469430 7_2:-24431309 Msa1053680:CDS
AGATGTATTCTGTAAGATCA+TGG 0.472448 7_2:+24431314 None:intergenic
TTCGTCCCAAATTTCAGTAC+CGG 0.474329 7_2:-24431163 Msa1053680:CDS
ATAACATAATGGCGGGGTGG+TGG 0.489266 7_2:-24436259 Msa1053680:exon
ATTCAACCTCAGCAATGGAC+TGG 0.500375 7_2:-24431515 Msa1053680:CDS
TCATATGGCGGAGAAGCAAT+CGG 0.500801 7_2:-24436191 Msa1053680:CDS
TTCAACCTCAGCAATGGACT+GGG 0.502524 7_2:-24431514 Msa1053680:CDS
GCCATATGATCGGAGTAGTA+AGG 0.504927 7_2:+24436205 None:intergenic
CCTAACTTCAGTAGCAGCTA+AGG 0.506076 7_2:+24431380 None:intergenic
GATGTATTCTGTAAGATCAT+GGG 0.518629 7_2:+24431315 None:intergenic
CTAGATAAAATACCAACTAT+GGG 0.519361 7_2:+24431216 None:intergenic
CCTGCCAATGGAGAAAGATA+AGG 0.520262 7_2:+24435956 None:intergenic
TTGCTTCTCCGCCATATGAT+CGG 0.526914 7_2:+24436195 None:intergenic
CTCGCTCAACTCCATTTGCA+AGG 0.541452 7_2:+24431466 None:intergenic
GTATGAATAGCGATGTATAG+TGG 0.550765 7_2:+24436095 None:intergenic
AAAATTATCTAGTAGTGAAG+AGG 0.553803 7_2:-24431092 Msa1053680:three_prime_UTR
AATCATATGTTACCTGCCAA+TGG 0.556218 7_2:+24435944 None:intergenic
AGAGATTTATCTGATAAACT+AGG 0.558993 7_2:-24436131 Msa1053680:CDS
GGGATGCATCCGACAATGGT+AGG 0.579071 7_2:+24431336 None:intergenic
TTGAGATTTCTTAGAGCCAG+AGG 0.582555 7_2:+24431257 None:intergenic
TCTAGCCCAGTCCATTGCTG+AGG 0.592500 7_2:+24431509 None:intergenic
ATGGAGTTGAGCGAGATGGT+TGG 0.593190 7_2:-24431458 Msa1053680:CDS
CAGGAATTCAACCTCAGCAA+TGG 0.593899 7_2:-24431520 Msa1053680:CDS
TCCCAAACAGACAGTTCGAT+CGG 0.605038 7_2:+24431006 None:intergenic
CATGGGGATGCATCCGACAA+TGG 0.608315 7_2:+24431332 None:intergenic
ATGTATTCTGTAAGATCATG+GGG 0.610828 7_2:+24431316 None:intergenic
TTAAATCAATAACATAATGG+CGG 0.617001 7_2:-24436267 Msa1053680:exon
TCTGTTAGTAAACTGGTGCG+TGG 0.633463 7_2:-24431058 Msa1053680:three_prime_UTR
GTATAGTTAATGATATAGGA+TGG 0.638706 7_2:+24431402 None:intergenic
GCAAATGGAGTTGAGCGAGA+TGG 0.646060 7_2:-24431462 Msa1053680:CDS
CAGTTCGATCGGTAAACTGA+AGG 0.647256 7_2:+24431017 None:intergenic
AAATCAATAACATAATGGCG+GGG 0.648875 7_2:-24436265 Msa1053680:exon
TTAATGATATAGGATGGCAC+AGG 0.650154 7_2:+24431408 None:intergenic
GGGGTGGTGGTGGAAAACAG+CGG 0.679901 7_2:-24436246 Msa1053680:CDS
TAGATAAAATACCAACTATG+GGG 0.680141 7_2:+24431217 None:intergenic
TCAATAACATAATGGCGGGG+TGG 0.686065 7_2:-24436262 Msa1053680:exon
TCGATCGGTAAACTGAAGGA+CGG 0.705675 7_2:+24431021 None:intergenic
TTACTACTCCGATCATATGG+CGG 0.710739 7_2:-24436203 Msa1053680:CDS
ATATGATCGGAGTAGTAAGG+CGG 0.729764 7_2:+24436208 None:intergenic
AGAAAGATAAGGAAGCAGCG+AGG 0.748647 7_2:+24435967 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATAGTTGATTTAATAATTA+AGG + chr7_2:24432754-24432773 None:intergenic 10.0%
!!! TTAATTTATTATGTTGTTTT+AGG - chr7_2:24432772-24432791 Msa1053680:intron 10.0%
!! TAGTCATTAATATTAATAAT+AGG + chr7_2:24433876-24433895 None:intergenic 10.0%
!! AAATAAACATGTTTAAATTT+AGG - chr7_2:24434708-24434727 Msa1053680:intron 10.0%
!!! AATTTTTCAACAAAATTTTA+AGG + chr7_2:24434808-24434827 None:intergenic 10.0%
!! GAATTAAATCAATAACATAA+TGG - chr7_2:24430969-24430988 Msa1053680:three_prime_UTR 15.0%
!! TAGAAACTTAGAATTAAAAA+AGG - chr7_2:24431496-24431515 Msa1053680:CDS 15.0%
!! AGAAACTTAGAATTAAAAAA+GGG - chr7_2:24431497-24431516 Msa1053680:CDS 15.0%
!!! TAGAATTAGTTGAATAATTT+TGG - chr7_2:24431707-24431726 Msa1053680:intron 15.0%
!! TATATTATTAAATGTGTGTT+TGG - chr7_2:24432124-24432143 Msa1053680:intron 15.0%
!!! TGATTTTGTAAATTTGATTT+TGG - chr7_2:24432179-24432198 Msa1053680:intron 15.0%
!!! GAATAAATTTGAGTTAAATA+TGG - chr7_2:24432252-24432271 Msa1053680:intron 15.0%
!!! AATTTTTGTTTTTTCAAAAG+TGG - chr7_2:24432391-24432410 Msa1053680:intron 15.0%
!! TTAAATAAACTAATTGTGTT+TGG - chr7_2:24432858-24432877 Msa1053680:intron 15.0%
!!! TTTTGTAGTTTTTTAAATTG+TGG - chr7_2:24433022-24433041 Msa1053680:intron 15.0%
!! TTAATTCATTAAATTGTGAT+AGG - chr7_2:24433443-24433462 Msa1053680:intron 15.0%
!! AAAAAAATACAAAGAATTTG+AGG - chr7_2:24433586-24433605 Msa1053680:intron 15.0%
!! TTAAATCAATAACATAATGG+CGG - chr7_2:24430972-24430991 Msa1053680:three_prime_UTR 20.0%
!! ATAAAGAATCATTGATGAAA+TGG - chr7_2:24431082-24431101 Msa1053680:three_prime_UTR 20.0%
!!! TTTCTTTTTCACATTTTCAT+CGG + chr7_2:24431402-24431421 None:intergenic 20.0%
!!! TAGTTGAATAATTTTGGTTA+AGG - chr7_2:24431713-24431732 Msa1053680:intron 20.0%
!! ATATTTATAAGCTACTTCAA+CGG + chr7_2:24431846-24431865 None:intergenic 20.0%
!!! TTGTTTTCAATTAAGTTTGT+AGG - chr7_2:24431900-24431919 Msa1053680:intron 20.0%
!!! TGTTTTCAATTAAGTTTGTA+GGG - chr7_2:24431901-24431920 Msa1053680:intron 20.0%
!!! GGTTTAAGTTTAATCAATTA+TGG - chr7_2:24432303-24432322 Msa1053680:intron 20.0%
!! TTAAGTTTAATCAATTATGG+AGG - chr7_2:24432306-24432325 Msa1053680:intron 20.0%
!!! AAAACAAAAATTGATTCTAG+AGG + chr7_2:24432383-24432402 None:intergenic 20.0%
!! TAATTGGATGTAAATTAGTT+TGG - chr7_2:24432597-24432616 Msa1053680:intron 20.0%
!! ATGATATGATGAATTCTAAT+TGG - chr7_2:24432645-24432664 Msa1053680:intron 20.0%
!! AAATTAACAACTAAAATGCT+AGG + chr7_2:24433150-24433169 None:intergenic 20.0%
!! AAACTATCAAATAAAACTCT+TGG + chr7_2:24433403-24433422 None:intergenic 20.0%
!! CTTAATAAACTATTTGTGTT+TGG - chr7_2:24433535-24433554 Msa1053680:intron 20.0%
!! CATACATGTTTATATCATTA+AGG - chr7_2:24434019-24434038 Msa1053680:intron 20.0%
!! AATCTTTGTAATCAAATGAT+TGG - chr7_2:24434209-24434228 Msa1053680:intron 20.0%
!!! TGTTTTTTTTTTGTTATGCT+TGG - chr7_2:24434424-24434443 Msa1053680:intron 20.0%
!!! AAATATTCTATGGTAATGAA+GGG - chr7_2:24434468-24434487 Msa1053680:intron 20.0%
!!! GCTTATTTTTGTTTTGTATA+CGG - chr7_2:24434512-24434531 Msa1053680:intron 20.0%
!! TGAGTTAAAATTGATAGAAT+AGG - chr7_2:24434616-24434635 Msa1053680:intron 20.0%
!! TGTTGAAAAATTTGATAAGT+TGG - chr7_2:24434816-24434835 Msa1053680:intron 20.0%
!!! CTTCTAAAATAAAAAATCAC+AGG + chr7_2:24434923-24434942 None:intergenic 20.0%
!! TTTATAAAAATGTATCCTCT+TGG - chr7_2:24435012-24435031 Msa1053680:intron 20.0%
!! AATTATAAGTGGTAGTTTAT+AGG - chr7_2:24435298-24435317 Msa1053680:intron 20.0%
!! ATTTGAGAAAATGGATAAAT+TGG + chr7_2:24435524-24435543 None:intergenic 20.0%
!! GTGATAAATATTTGAGAAAA+TGG + chr7_2:24435533-24435552 None:intergenic 20.0%
!! TTTATTAGTAACAACTAACA+AGG - chr7_2:24435579-24435598 Msa1053680:intron 20.0%
!! GACAATACATTATTAACATA+TGG + chr7_2:24435609-24435628 None:intergenic 20.0%
!!! TATTTTAAAATCCTTGCAAA+TGG - chr7_2:24435762-24435781 Msa1053680:intron 20.0%
!! TAAGGTATAGTTAATGATAT+AGG + chr7_2:24435844-24435863 None:intergenic 20.0%
!! AATACTAACAACAACAAAAT+TGG + chr7_2:24436292-24436311 None:intergenic 20.0%
!! AAGAGTATAGTCAACAAAAA+CGG - chr7_2:24430897-24430916 Msa1053680:three_prime_UTR 25.0%
! TAAATCAATAACATAATGGC+GGG - chr7_2:24430973-24430992 Msa1053680:three_prime_UTR 25.0%
! AGAGATTTATCTGATAAACT+AGG - chr7_2:24431108-24431127 Msa1053680:exon 25.0%
! TATCTGATAAACTAGGAATT+TGG - chr7_2:24431115-24431134 Msa1053680:CDS 25.0%
! ATCTGATAAACTAGGAATTT+GGG - chr7_2:24431116-24431135 Msa1053680:CDS 25.0%
!!! GTTTTTGGTAAAAAAAGTAG+CGG - chr7_2:24431748-24431767 Msa1053680:intron 25.0%
! ACTGATAAGATAGTTTATAG+TGG - chr7_2:24431774-24431793 Msa1053680:intron 25.0%
! ACTTATAAGCTAACTTATAG+CGG - chr7_2:24431800-24431819 Msa1053680:intron 25.0%
!! GTTTTCAATTAAGTTTGTAG+GGG - chr7_2:24431902-24431921 Msa1053680:intron 25.0%
!! TTATTTTCATGAGCTTATAG+AGG + chr7_2:24431984-24432003 None:intergenic 25.0%
! AAGACATAAAATTGACCTTA+TGG + chr7_2:24432082-24432101 None:intergenic 25.0%
! CGATAAATTTAGTGTATGTT+TGG + chr7_2:24432419-24432438 None:intergenic 25.0%
!!! ATTTTTTGTCGATGAGAAAA+AGG - chr7_2:24432475-24432494 Msa1053680:intron 25.0%
!!! TGTAGTTATTTGTTATGTTG+TGG - chr7_2:24432538-24432557 Msa1053680:intron 25.0%
! AAATTGACAACTAAAACACT+AGG + chr7_2:24432730-24432749 None:intergenic 25.0%
!!! TTATGTTGTTTTAGGAAGTA+AGG - chr7_2:24432780-24432799 Msa1053680:intron 25.0%
! TTAAATTGTGATAGGAAGTT+AGG - chr7_2:24433451-24433470 Msa1053680:intron 25.0%
! GTTTATTAAGTTAAAGAGCT+TGG + chr7_2:24433525-24433544 None:intergenic 25.0%
! AAAATACAAAGAATTTGAGG+TGG - chr7_2:24433589-24433608 Msa1053680:intron 25.0%
! TGTACTATTATGTAATGCTT+GGG - chr7_2:24433746-24433765 Msa1053680:intron 25.0%
!!! TCTTTTGTATGTCGATTTAT+AGG - chr7_2:24434055-24434074 Msa1053680:intron 25.0%
!! AAATTTTGTGGTTTCTTTGT+TGG - chr7_2:24434085-24434104 Msa1053680:intron 25.0%
! TGAAGCTTATATTAAAAGCA+GGG - chr7_2:24434117-24434136 Msa1053680:intron 25.0%
! TCATTTGATTACAAAGATTG+AGG + chr7_2:24434208-24434227 None:intergenic 25.0%
! TTTGTAATCAAATGATTGGT+TGG - chr7_2:24434213-24434232 Msa1053680:intron 25.0%
!! TAATTTGAGATGAAAGTTAC+TGG - chr7_2:24434238-24434257 Msa1053680:intron 25.0%
! TAGAATATTTGCACAGAAAA+AGG + chr7_2:24434458-24434477 None:intergenic 25.0%
!! TTTTCTGTGCAAATATTCTA+TGG - chr7_2:24434458-24434477 Msa1053680:intron 25.0%
!! CAAATATTCTATGGTAATGA+AGG - chr7_2:24434467-24434486 Msa1053680:intron 25.0%
!! AGTTTTGCATCTTCATTTTA+CGG - chr7_2:24434648-24434667 Msa1053680:intron 25.0%
!!! AAATTTGATAAGTTGGTTGA+AGG - chr7_2:24434823-24434842 Msa1053680:intron 25.0%
! CTATACCGAATTATATCATT+GGG - chr7_2:24435109-24435128 Msa1053680:intron 25.0%
! ATATATTGTCTGATTCAAGT+TGG - chr7_2:24435145-24435164 Msa1053680:intron 25.0%
! AAAGAAGCAATAACTTAAAC+TGG + chr7_2:24435238-24435257 None:intergenic 25.0%
! GTCATTGTATGAATTATAAG+TGG - chr7_2:24435287-24435306 Msa1053680:intron 25.0%
! TTGGATGTAATAATATGTGT+CGG + chr7_2:24435437-24435456 None:intergenic 25.0%
! TTGGATGTAATAATATGTGT+CGG + chr7_2:24435437-24435456 None:intergenic 25.0%
! GTATAGTTAATGATATAGGA+TGG + chr7_2:24435840-24435859 None:intergenic 25.0%
! ATCTTACAGAATACATCTAT+AGG - chr7_2:24435930-24435949 Msa1053680:intron 25.0%
! TAGATAAAATACCAACTATG+GGG + chr7_2:24436025-24436044 None:intergenic 25.0%
! CTAGATAAAATACCAACTAT+GGG + chr7_2:24436026-24436045 None:intergenic 25.0%
! ACTAGATAAAATACCAACTA+TGG + chr7_2:24436027-24436046 None:intergenic 25.0%
! AAAATTATCTAGTAGTGAAG+AGG - chr7_2:24436147-24436166 Msa1053680:CDS 25.0%
!! ATGTTTTTCTGTTAGTAAAC+TGG - chr7_2:24436174-24436193 Msa1053680:CDS 25.0%
!! AATTCAGCGTTGAAGATTTT+AGG + chr7_2:24430954-24430973 None:intergenic 30.0%
AAATCAATAACATAATGGCG+GGG - chr7_2:24430974-24430993 Msa1053680:three_prime_UTR 30.0%
GTTTCAAGAAAAATAGCAGA+AGG + chr7_2:24431192-24431211 None:intergenic 30.0%
!!! TCTGCTATTTTTCTTGAAAC+TGG - chr7_2:24431192-24431211 Msa1053680:CDS 30.0%
TTCTAAGTTTCTAACAAGAG+AGG + chr7_2:24431692-24431711 None:intergenic 30.0%
!!! AGGTAGTTGTGATTAGTTTT+TGG - chr7_2:24431733-24431752 Msa1053680:intron 30.0%
TTAAGTTTGTAGGGGTAAAA+TGG - chr7_2:24431910-24431929 Msa1053680:intron 30.0%
ATAGAATGCTATAAGCCATA+AGG - chr7_2:24432064-24432083 Msa1053680:intron 30.0%
TTACTAAATAGACATCTAGC+TGG - chr7_2:24432100-24432119 Msa1053680:intron 30.0%
AACGTGAAGTGATTTATGTT+TGG - chr7_2:24432205-24432224 Msa1053680:intron 30.0%
AGTCGAAAGCTACAAATATT+AGG - chr7_2:24432282-24432301 Msa1053680:intron 30.0%
CGATGAGAAAAAGGAATAAT+AGG - chr7_2:24432484-24432503 Msa1053680:intron 30.0%
AGAAAAAGGAATAATAGGTG+TGG - chr7_2:24432489-24432508 Msa1053680:intron 30.0%
GATGAATTCTAATTGGATAC+TGG - chr7_2:24432652-24432671 Msa1053680:intron 30.0%
AAACACTAGGTATCATCAAA+GGG + chr7_2:24432717-24432736 None:intergenic 30.0%
AAAACACTAGGTATCATCAA+AGG + chr7_2:24432718-24432737 None:intergenic 30.0%
!! AATTGATGGTGTTTGGTAAA+TGG - chr7_2:24432806-24432825 Msa1053680:intron 30.0%
! TTTTATAGTGATGTCCAATG+AGG - chr7_2:24432990-24433009 Msa1053680:intron 30.0%
CAAAAATACTAGAACCTCAT+TGG + chr7_2:24433007-24433026 None:intergenic 30.0%
CGATAATGATGACTTCTAAT+TGG - chr7_2:24433065-24433084 Msa1053680:intron 30.0%
! AGTTTTACACTGATGTTCAA+CGG - chr7_2:24433266-24433285 Msa1053680:intron 30.0%
GTGTACTATTATGTAATGCT+TGG - chr7_2:24433745-24433764 Msa1053680:intron 30.0%
GTACTATTATGTAATGCTTG+GGG - chr7_2:24433747-24433766 Msa1053680:intron 30.0%
ATGAGTCATACTCAACTAAT+TGG - chr7_2:24433895-24433914 Msa1053680:intron 30.0%
TGAGTCATACTCAACTAATT+GGG - chr7_2:24433896-24433915 Msa1053680:intron 30.0%
AAGCTCTCTCATATAGATTA+CGG + chr7_2:24433953-24433972 None:intergenic 30.0%
AGAAACCACAAAATTTCTGA+TGG + chr7_2:24434081-24434100 None:intergenic 30.0%
GTGAAGCTTATATTAAAAGC+AGG - chr7_2:24434116-24434135 Msa1053680:intron 30.0%
TTGATCATGATAGACAAGAT+TGG + chr7_2:24434321-24434340 None:intergenic 30.0%
ATCAATCATGCAAACAGAAT+TGG - chr7_2:24434336-24434355 Msa1053680:intron 30.0%
!!! TTTTTTGTTATGCTTGGCTT+TGG - chr7_2:24434430-24434449 Msa1053680:intron 30.0%
CGGAAGTTATATTCTTTCTT+TGG - chr7_2:24434532-24434551 Msa1053680:intron 30.0%
!! TTTCTTTGGTCTTTCTTTTC+TGG - chr7_2:24434546-24434565 Msa1053680:intron 30.0%
TGAGTGTAATATCAGCAATA+AGG + chr7_2:24434870-24434889 None:intergenic 30.0%
CGGTATAGCTAAATATGTTA+AGG + chr7_2:24435097-24435116 None:intergenic 30.0%
GCTATACCGAATTATATCAT+TGG - chr7_2:24435108-24435127 Msa1053680:intron 30.0%
CCTTTCCCAATGATATAATT+CGG + chr7_2:24435117-24435136 None:intergenic 30.0%
CGAATTATATCATTGGGAAA+GGG - chr7_2:24435115-24435134 Msa1053680:intron 30.0%
TATTCCAGTGAAGCTATAAA+GGG + chr7_2:24435194-24435213 None:intergenic 30.0%
! TTGCTTCTTTGACTTTACTT+TGG - chr7_2:24435248-24435267 Msa1053680:intron 30.0%
GTTTATAGGATGAATGAATG+AGG - chr7_2:24435312-24435331 Msa1053680:intron 30.0%
!! ATGCCTTTAAAGTGAAATGT+TGG + chr7_2:24435406-24435425 None:intergenic 30.0%
! ATTTCACTTTAAAGGCATGT+CGG - chr7_2:24435408-24435427 Msa1053680:intron 30.0%
GTTAATAATGTATTGTCCTC+TGG - chr7_2:24435612-24435631 Msa1053680:intron 30.0%
ATTACATATAGAACATCCAG+AGG + chr7_2:24435631-24435650 None:intergenic 30.0%
CATAAATTCAGATCAGATCA+GGG + chr7_2:24435672-24435691 None:intergenic 30.0%
ACATAAATTCAGATCAGATC+AGG + chr7_2:24435673-24435692 None:intergenic 30.0%
!!! TATGTCATTTTGAGTTCTTC+AGG - chr7_2:24435688-24435707 Msa1053680:intron 30.0%
ATGTATTCTGTAAGATCATG+GGG + chr7_2:24435926-24435945 None:intergenic 30.0%
GATGTATTCTGTAAGATCAT+GGG + chr7_2:24435927-24435946 None:intergenic 30.0%
AGATGTATTCTGTAAGATCA+TGG + chr7_2:24435928-24435947 None:intergenic 30.0%
!!! GCTCTAAGAAATCTCAATTT+TGG - chr7_2:24435988-24436007 Msa1053680:CDS 30.0%
! AATTTCCGGTACTGAAATTT+GGG + chr7_2:24436084-24436103 None:intergenic 30.0%
! AAATTTCCGGTACTGAAATT+TGG + chr7_2:24436085-24436104 None:intergenic 30.0%
TCTTTGTCAGAAGAAATTTC+CGG + chr7_2:24436098-24436117 None:intergenic 30.0%
GTATGAATAGCGATGTATAG+TGG + chr7_2:24431147-24431166 None:intergenic 35.0%
AATCATATGTTACCTGCCAA+TGG + chr7_2:24431298-24431317 None:intergenic 35.0%
TCAACAACACTTCTAATGGA+AGG + chr7_2:24431458-24431477 None:intergenic 35.0%
AGCATCAACAACACTTCTAA+TGG + chr7_2:24431462-24431481 None:intergenic 35.0%
AGAATTAAAAAAGGGCAACC+CGG - chr7_2:24431505-24431524 Msa1053680:CDS 35.0%
!! AACTTATAGCGGATAGTGTT+TGG - chr7_2:24431811-24431830 Msa1053680:intron 35.0%
AGCTTATGATGTGAATGATG+AGG - chr7_2:24432572-24432591 Msa1053680:intron 35.0%
TGTGAATGATGAGGTCTAAT+TGG - chr7_2:24432581-24432600 Msa1053680:intron 35.0%
AGGAAGTAAGGATCAATTGA+TGG - chr7_2:24432792-24432811 Msa1053680:intron 35.0%
!! AAGGATCAATTGATGGTGTT+TGG - chr7_2:24432799-24432818 Msa1053680:intron 35.0%
GATCTGTTGACAGTGTAAAA+CGG - chr7_2:24432926-24432945 Msa1053680:intron 35.0%
! GTTTTACACTGATGTTCAAC+GGG - chr7_2:24433267-24433286 Msa1053680:intron 35.0%
ATAGGAAGTTAGGATTTAGC+AGG - chr7_2:24433461-24433480 Msa1053680:intron 35.0%
AGTTAGGATTTAGCAGGTTT+AGG - chr7_2:24433467-24433486 Msa1053680:intron 35.0%
GTTAGGATTTAGCAGGTTTA+GGG - chr7_2:24433468-24433487 Msa1053680:intron 35.0%
TTAGGATTTAGCAGGTTTAG+GGG - chr7_2:24433469-24433488 Msa1053680:intron 35.0%
CAAGTTGCGTTTAAATACTG+CGG - chr7_2:24433780-24433799 Msa1053680:intron 35.0%
GAGTCATACTCAACTAATTG+GGG - chr7_2:24433897-24433916 Msa1053680:intron 35.0%
AGTCATACTCAACTAATTGG+GGG - chr7_2:24433898-24433917 Msa1053680:intron 35.0%
! ATAGGCCATCAGAAATTTTG+TGG - chr7_2:24434073-24434092 Msa1053680:intron 35.0%
CCTTTCACAGAGACAATATT+AGG + chr7_2:24434373-24434392 None:intergenic 35.0%
CCTAATATTGTCTCTGTGAA+AGG - chr7_2:24434370-24434389 Msa1053680:intron 35.0%
TTGGTTTCTCATCCATAGTT+GGG - chr7_2:24435031-24435050 Msa1053680:intron 35.0%
CATGTAAGAAATCCCAACTA+TGG + chr7_2:24435046-24435065 None:intergenic 35.0%
CCGAATTATATCATTGGGAA+AGG - chr7_2:24435114-24435133 Msa1053680:intron 35.0%
CTATTCCAGTGAAGCTATAA+AGG + chr7_2:24435195-24435214 None:intergenic 35.0%
GCAATAACTTAAACTGGCAA+TGG + chr7_2:24435232-24435251 None:intergenic 35.0%
!! CCTTTAAAGTGAAATGTTGG+TGG + chr7_2:24435403-24435422 None:intergenic 35.0%
CCACCAACATTTCACTTTAA+AGG - chr7_2:24435400-24435419 Msa1053680:intron 35.0%
ATGCCTTAAATGTGAAGTGT+CGG + chr7_2:24435474-24435493 None:intergenic 35.0%
! ACTTCACATTTAAGGCATGT+CGG - chr7_2:24435476-24435495 Msa1053680:intron 35.0%
!! ATAAAGCACTGTCAAAGATC+TGG + chr7_2:24435564-24435583 None:intergenic 35.0%
AGATCAGGGAAGATAAAAAG+AGG + chr7_2:24435658-24435677 None:intergenic 35.0%
! AGTTCTTCAGGTTAGTATTC+AGG - chr7_2:24435700-24435719 Msa1053680:intron 35.0%
TTAATGATATAGGATGGCAC+AGG + chr7_2:24435834-24435853 None:intergenic 35.0%
TTTCTTCTTCCTACCATTGT+CGG - chr7_2:24435894-24435913 Msa1053680:intron 35.0%
!!! TCTTACTTTTTCCCCATAGT+TGG - chr7_2:24436011-24436030 Msa1053680:CDS 35.0%
ATATGATCGGAGTAGTAAGG+CGG + chr7_2:24431034-24431053 None:intergenic 40.0%
TTACTACTCCGATCATATGG+CGG - chr7_2:24431036-24431055 Msa1053680:three_prime_UTR 40.0%
GCTTCCTTATCTTTCTCCAT+TGG - chr7_2:24431279-24431298 Msa1053680:CDS 40.0%
CGTACAATACACCAAATGGT+GGG + chr7_2:24431571-24431590 None:intergenic 40.0%
GTTGCGTACAATACACCAAA+TGG + chr7_2:24431575-24431594 None:intergenic 40.0%
TCTGGTGTAAAAACAGGGTA+AGG + chr7_2:24431597-24431616 None:intergenic 40.0%
!!! TTACCCTGTTTTTACACCAG+AGG - chr7_2:24431596-24431615 Msa1053680:intron 40.0%
GATGTTGTCATGTGACTGTA+AGG + chr7_2:24431647-24431666 None:intergenic 40.0%
GGAGGCAGAATCAATTCTAT+CGG - chr7_2:24432324-24432343 Msa1053680:intron 40.0%
TAGTGTTAGAACAGCAAAGC+TGG - chr7_2:24433624-24433643 Msa1053680:intron 40.0%
TTACGTCTCCGTCAATAGAT+TGG - chr7_2:24433715-24433734 Msa1053680:intron 40.0%
CTCAGTAGCCAATCTATTGA+CGG + chr7_2:24433726-24433745 None:intergenic 40.0%
TAATAGGTCAAACCCTAGAG+CGG + chr7_2:24433860-24433879 None:intergenic 40.0%
ATACTCAACTAATTGGGGGA+TGG - chr7_2:24433902-24433921 Msa1053680:intron 40.0%
ACTATGGATGAGAAACCAAG+AGG + chr7_2:24435030-24435049 None:intergenic 40.0%
CTTGGTTTCTCATCCATAGT+TGG - chr7_2:24435030-24435049 Msa1053680:intron 40.0%
CATGGACTCAACATGCTTAA+AGG + chr7_2:24435171-24435190 None:intergenic 40.0%
!! AAAGTGAAATGTTGGTGGCT+CGG + chr7_2:24435398-24435417 None:intergenic 40.0%
! GGAAGTTTGTTCTTCTTGCT+CGG - chr7_2:24435802-24435821 Msa1053680:intron 40.0%
ACATCTATAGGAAGTCTTGC+TGG - chr7_2:24435942-24435961 Msa1053680:intron 40.0%
TTGAGATTTCTTAGAGCCAG+AGG + chr7_2:24435985-24436004 None:intergenic 40.0%
TTCGTCCCAAATTTCAGTAC+CGG - chr7_2:24436076-24436095 Msa1053680:CDS 40.0%
! TCAATAACATAATGGCGGGG+TGG - chr7_2:24430977-24430996 Msa1053680:three_prime_UTR 45.0%
GCCTTACTACTCCGATCATA+TGG - chr7_2:24431033-24431052 Msa1053680:three_prime_UTR 45.0%
GCCATATGATCGGAGTAGTA+AGG + chr7_2:24431037-24431056 None:intergenic 45.0%
TTGCTTCTCCGCCATATGAT+CGG + chr7_2:24431047-24431066 None:intergenic 45.0%
! TCATATGGCGGAGAAGCAAT+CGG - chr7_2:24431048-24431067 Msa1053680:three_prime_UTR 45.0%
ACTGGTGCTTCTCTTCTCTT+TGG - chr7_2:24431210-24431229 Msa1053680:CDS 45.0%
GTGCTTCTCTTCTCTTTGGT+TGG - chr7_2:24431214-24431233 Msa1053680:CDS 45.0%
AGAAAGATAAGGAAGCAGCG+AGG + chr7_2:24431275-24431294 None:intergenic 45.0%
CCTGCCAATGGAGAAAGATA+AGG + chr7_2:24431286-24431305 None:intergenic 45.0%
CCTTATCTTTCTCCATTGGC+AGG - chr7_2:24431283-24431302 Msa1053680:CDS 45.0%
GCGTACAATACACCAAATGG+TGG + chr7_2:24431572-24431591 None:intergenic 45.0%
ACGGGTTTAAGTCCTGGAAA+CGG + chr7_2:24431622-24431641 None:intergenic 45.0%
TCATGTGACTGTAAGGTCAC+GGG + chr7_2:24431640-24431659 None:intergenic 45.0%
GTCATGTGACTGTAAGGTCA+CGG + chr7_2:24431641-24431660 None:intergenic 45.0%
CATCTTTACCAGTTGCGCTA+AGG - chr7_2:24431663-24431682 Msa1053680:intron 45.0%
!! TTAGCAGGTTTAGGGGTGTT+TGG - chr7_2:24433476-24433495 Msa1053680:intron 45.0%
GACGGAGACGTAAGCATAAT+TGG + chr7_2:24433708-24433727 None:intergenic 45.0%
CTAAAAGTAGGCAGACGGTT+CGG + chr7_2:24433826-24433845 None:intergenic 45.0%
TGGAGCTAAAAGTAGGCAGA+CGG + chr7_2:24433831-24433850 None:intergenic 45.0%
GAGCAAGTGGAGCTAAAAGT+AGG + chr7_2:24433838-24433857 None:intergenic 45.0%
AAAGGACTGCTGGGAACATT+AGG - chr7_2:24434388-24434407 Msa1053680:intron 45.0%
! TCTATGGTAATGAAGGGTGC+TGG - chr7_2:24434474-24434493 Msa1053680:intron 45.0%
CAGTGAAGCTATAAAGGGCA+TGG + chr7_2:24435189-24435208 None:intergenic 45.0%
CATGCCCTTTATAGCTTCAC+TGG - chr7_2:24435187-24435206 Msa1053680:intron 45.0%
!! GTCGGTGCTACAGGATAAAT+TGG + chr7_2:24435456-24435475 None:intergenic 45.0%
! GCACCGACACTTCACATTTA+AGG - chr7_2:24435468-24435487 Msa1053680:intron 45.0%
CAGGAATTCAACCTCAGCAA+TGG - chr7_2:24435719-24435738 Msa1053680:intron 45.0%
ATTCAACCTCAGCAATGGAC+TGG - chr7_2:24435724-24435743 Msa1053680:intron 45.0%
! TTCAACCTCAGCAATGGACT+GGG - chr7_2:24435725-24435744 Msa1053680:intron 45.0%
TCTGTTAGTAAACTGGTGCG+TGG - chr7_2:24436181-24436200 Msa1053680:CDS 45.0%
TCGATCGGTAAACTGAAGGA+CGG + chr7_2:24436221-24436240 None:intergenic 45.0%
CAGTTCGATCGGTAAACTGA+AGG + chr7_2:24436225-24436244 None:intergenic 45.0%
TACCGATCGAACTGTCTGTT+TGG - chr7_2:24436231-24436250 Msa1053680:CDS 45.0%
ACCGATCGAACTGTCTGTTT+GGG - chr7_2:24436232-24436251 Msa1053680:CDS 45.0%
TCCCAAACAGACAGTTCGAT+CGG + chr7_2:24436236-24436255 None:intergenic 45.0%
! ATAACATAATGGCGGGGTGG+TGG - chr7_2:24430980-24430999 Msa1053680:three_prime_UTR 50.0%
!! ATGAAGCGCTGACACAGACA+CGG - chr7_2:24431365-24431384 Msa1053680:CDS 50.0%
ACTGAAGTTCCCGCATACAC+AGG - chr7_2:24431531-24431550 Msa1053680:CDS 50.0%
CTGAAGTTCCCGCATACACA+GGG - chr7_2:24431532-24431551 Msa1053680:CDS 50.0%
CGGCCTCTGGTGTAAAAACA+GGG + chr7_2:24431602-24431621 None:intergenic 50.0%
ACGGCCTCTGGTGTAAAAAC+AGG + chr7_2:24431603-24431622 None:intergenic 50.0%
AAGGTCACGGGTTTAAGTCC+TGG + chr7_2:24431628-24431647 None:intergenic 50.0%
AGAGGTAACCTTAGCGCAAC+TGG + chr7_2:24431674-24431693 None:intergenic 50.0%
CTTATTCCCTCTGCTTCCTC+TGG + chr7_2:24434275-24434294 None:intergenic 50.0%
TGTCTCTGTGAAAGGACTGC+TGG - chr7_2:24434378-24434397 Msa1053680:intron 50.0%
GTCTCTGTGAAAGGACTGCT+GGG - chr7_2:24434379-24434398 Msa1053680:intron 50.0%
!! ATGTGAAGTGTCGGTGCTAC+AGG + chr7_2:24435465-24435484 None:intergenic 50.0%
CTCGCTCAACTCCATTTGCA+AGG + chr7_2:24435776-24435795 None:intergenic 50.0%
GCAAATGGAGTTGAGCGAGA+TGG - chr7_2:24435777-24435796 Msa1053680:intron 50.0%
ATGGAGTTGAGCGAGATGGT+TGG - chr7_2:24435781-24435800 Msa1053680:intron 50.0%
GGCGGAGAAGCAATCGGAAT+CGG - chr7_2:24431054-24431073 Msa1053680:three_prime_UTR 55.0%
ATGCGGGAACTTCAGTGCAC+CGG + chr7_2:24431527-24431546 None:intergenic 55.0%
TTACACCAGAGGCCGTTTCC+AGG - chr7_2:24431607-24431626 Msa1053680:intron 55.0%
TAAGTCCTGGAAACGGCCTC+TGG + chr7_2:24431615-24431634 None:intergenic 55.0%
! AAAGTAGGCAGACGGTTCGG+CGG + chr7_2:24433823-24433842 None:intergenic 55.0%
AAACCCTAGAGCGGAGCAAG+TGG + chr7_2:24433851-24433870 None:intergenic 55.0%
! ACTGGCTTCTCCTGTACCAG+AGG - chr7_2:24434256-24434275 Msa1053680:intron 55.0%
TCCTGTACCAGAGGAAGCAG+AGG - chr7_2:24434265-24434284 Msa1053680:intron 55.0%
CCTGTACCAGAGGAAGCAGA+GGG - chr7_2:24434266-24434285 Msa1053680:intron 55.0%
TCTAGCCCAGTCCATTGCTG+AGG + chr7_2:24435733-24435752 None:intergenic 55.0%
!! CAGCAATGGACTGGGCTAGA+AGG - chr7_2:24435733-24435752 Msa1053680:intron 55.0%
GGGATGCATCCGACAATGGT+AGG + chr7_2:24435906-24435925 None:intergenic 55.0%
CATGGGGATGCATCCGACAA+TGG + chr7_2:24435910-24435929 None:intergenic 55.0%
! ACATAATGGCGGGGTGGTGG+TGG - chr7_2:24430983-24431002 Msa1053680:three_prime_UTR 60.0%
!! GGGGTGGTGGTGGAAAACAG+CGG - chr7_2:24430993-24431012 Msa1053680:three_prime_UTR 60.0%
TGCGGGAACTTCAGTGCACC+GGG + chr7_2:24431526-24431545 None:intergenic 60.0%
GTTCCCGCATACACAGGGTC+CGG - chr7_2:24431537-24431556 Msa1053680:intron 60.0%
TTCCCGCATACACAGGGTCC+GGG - chr7_2:24431538-24431557 Msa1053680:intron 60.0%
TTCCCGGACCCTGTGTATGC+GGG + chr7_2:24431543-24431562 None:intergenic 60.0%
CTTCCCGGACCCTGTGTATG+CGG + chr7_2:24431544-24431563 None:intergenic 60.0%
GCATACACAGGGTCCGGGAA+GGG - chr7_2:24431543-24431562 Msa1053680:intron 60.0%
CATACACAGGGTCCGGGAAG+GGG - chr7_2:24431544-24431563 Msa1053680:intron 60.0%
! CAAATGGTGGGACCCCTTCC+CGG + chr7_2:24431559-24431578 None:intergenic 60.0%
AGCTCCACTTGCTCCGCTCT+AGG - chr7_2:24433844-24433863 Msa1053680:intron 60.0%
GCTCCACTTGCTCCGCTCTA+GGG - chr7_2:24433845-24433864 Msa1053680:intron 60.0%
CCCTCTGCTTCCTCTGGTAC+AGG + chr7_2:24434269-24434288 None:intergenic 60.0%
GCAGCTGTTGCTACAGCCTC+TGG - chr7_2:24435966-24435985 Msa1053680:CDS 60.0%
CGCATACACAGGGTCCGGGA+AGG - chr7_2:24431542-24431561 Msa1053680:intron 65.0%
CGGGAAGGGGTCCCACCATT+TGG - chr7_2:24431557-24431576 Msa1053680:intron 65.0%
Chromosome Type Strat End Strand Name
chr7_2 gene 24430878 24436383 24430878 ID=Msa1053680;Name=Msa1053680
chr7_2 mRNA 24430878 24436383 24430878 ID=Msa1053680-mRNA-1;Parent=Msa1053680;Name=Msa1053680-mRNA-1;_AED=0.01;_eAED=0.01;_QI=110|1|1|1|1|1|2|235|251
chr7_2 exon 24430878 24431551 24430878 ID=Msa1053680-mRNA-1:exon:5300;Parent=Msa1053680-mRNA-1
chr7_2 exon 24435957 24436383 24435957 ID=Msa1053680-mRNA-1:exon:5299;Parent=Msa1053680-mRNA-1
chr7_2 five_prime_UTR 24436274 24436383 24436274 ID=Msa1053680-mRNA-1:five_prime_utr;Parent=Msa1053680-mRNA-1
chr7_2 CDS 24435957 24436273 24435957 ID=Msa1053680-mRNA-1:cds;Parent=Msa1053680-mRNA-1
chr7_2 CDS 24431113 24431551 24431113 ID=Msa1053680-mRNA-1:cds;Parent=Msa1053680-mRNA-1
chr7_2 three_prime_UTR 24430878 24431112 24430878 ID=Msa1053680-mRNA-1:three_prime_utr;Parent=Msa1053680-mRNA-1
Gene Sequence

>Msa1053680

ATGGCGGGGTGGTGGTGGAAAACAGCGGTGTTAATAGCAATATCCGCCTTACTACTCCGATCATATGGCGGAGAAGCAATCGGAATCGGAATCGATAAAGAATCATTGATGAAATGGATGAGAGATTTATCTGATAAACTAGGAATTTGGGCGATTCCACTATACATCGCTATTCATACAATTTCAATTGCTCTTTGTTTACCTTCTGCTATTTTTCTTGAAACTGGTGCTTCTCTTCTCTTTGGTTGGTTTGCTTCTGTTCTTTGTGTTTTCTCTGCTAAGATCCTCGCTGCTTCCTTATCTTTCTCCATTGGCAGGTTAGTATTCAGGAATTCAACCTCAGCAATGGACTGGGCTAGAAGGAACAAATATTTTAAAATCCTTGCAAATGGAGTTGAGCGAGATGGTTGGAAGTTTGTTCTTCTTGCTCGGTTCTCGCCTGTGCCATCCTATATCATTAACTATACCTTAGCTGCTACTGAAGTTAGGTTCTTTTTGGATTTTCTTCTTCCTACCATTGTCGGATGCATCCCCATGATCTTACAGAATACATCTATAGGAAGTCTTGCTGGTGCAGCTGTTGCTACAGCCTCTGGCTCTAAGAAATCTCAATTTTGGTCTTACTTTTTCCCCATAGTTGGTATTTTATCTAGTGTACTTATTTCTTTGAGAATTAAAAAGTATTCGTCCCAAATTTCAGTACCGGAAATTTCTTCTGACAAAGATAACACTGTTGAGTCAAAACAACAAAAATAA
Protein sequence

>Msa1053680

MAGWWWKTAVLIAISALLLRSYGGEAIGIGIDKESLMKWMRDLSDKLGIWAIPLYIAIHTISIALCLPSAIFLETGASLLFGWFASVLCVFSAKILAASLSFSIGRLVFRNSTSAMDWARRNKYFKILANGVERDGWKFVLLARFSPVPSYIINYTLAATEVRFFLDFLLPTIVGCIPMILQNTSIGSLAGAAVATASGSKKSQFWSYFFPIVGILSSVLISLRIKKYSSQISVPEISSDKDNTVESKQQK*