AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1093220


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1093220 MtrunA17_Chr4g0064791 94.595 74 4 0 1 74 112 185 7.88e-46 145
Msa1093220 MtrunA17_Chr3g0139081 71.233 73 21 0 2 74 237 309 8.16e-32 115
Msa1093220 MtrunA17_Chr4g0000581 65.753 73 25 0 2 74 252 324 4.62e-29 107
Msa1093220 MtrunA17_Chr6g0451381 58.904 73 30 0 2 74 218 290 3.42e-24 93.6
Msa1093220 MtrunA17_Chr3g0135191 54.795 73 33 0 2 74 252 324 1.04e-21 86.7
Msa1093220 MtrunA17_Chr8g0339101 54.795 73 33 0 2 74 255 327 1.20e-20 83.6
Msa1093220 MtrunA17_Chr1g0198111 40.506 79 41 1 2 74 109 187 1.12e-13 63.9
Msa1093220 MtrunA17_Chr1g0179441 41.772 79 40 1 2 74 102 180 1.81e-13 63.2
Msa1093220 MtrunA17_Chr7g0265711 42.466 73 42 0 2 74 111 183 4.91e-13 62.0
Msa1093220 MtrunA17_Chr4g0027221 38.356 73 45 0 2 74 111 183 9.09e-13 61.2
Msa1093220 MtrunA17_Chr1g0179381 39.241 79 42 1 2 74 102 180 5.64e-12 58.9
Msa1093220 MtrunA17_Chr1g0179421 39.241 79 42 1 2 74 102 180 6.46e-12 58.9
Msa1093220 MtrunA17_Chr7g0264621 38.356 73 45 0 2 74 107 179 1.09e-11 58.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1093220 AT4G31590.1 64.384 73 26 0 2 74 242 314 1.42e-27 103
Msa1093220 AT2G24630.1 64.384 73 26 0 2 74 242 314 3.38e-27 102
Msa1093220 AT2G24630.2 64.384 73 26 0 2 74 242 314 3.38e-27 102
Msa1093220 AT3G28180.1 60.274 73 29 0 2 74 214 286 3.25e-23 91.3
Msa1093220 AT3G07330.1 53.425 73 34 0 2 74 236 308 1.08e-21 87.0
Msa1093220 AT3G07330.2 53.425 73 34 0 2 74 236 308 1.08e-21 87.0
Msa1093220 AT4G07960.1 52.055 73 35 0 2 74 256 328 2.44e-20 83.2
Msa1093220 AT4G07960.2 52.055 73 35 0 2 74 256 328 2.44e-20 83.2
Msa1093220 AT5G03760.1 43.038 79 39 1 2 74 112 190 1.73e-13 63.5
Msa1093220 AT4G13410.2 41.096 73 43 0 2 74 126 198 6.19e-13 62.0
Msa1093220 AT4G13410.1 41.096 73 43 0 2 74 126 198 1.08e-12 61.6
Msa1093220 AT5G22740.1 38.356 73 45 0 2 74 112 184 4.09e-12 59.7
Msa1093220 AT1G24070.2 41.667 72 42 0 3 74 138 209 4.86e-12 59.7
Msa1093220 AT1G24070.1 41.667 72 42 0 3 74 138 209 5.65e-12 59.3
Msa1093220 AT2G35650.1 39.744 78 41 1 3 74 135 212 8.77e-12 58.9
Msa1093220 AT2G35650.4 39.744 78 41 1 3 74 63 140 1.24e-11 58.5
Msa1093220 AT2G35650.2 39.744 78 41 1 3 74 63 140 1.24e-11 58.5
Msa1093220 AT5G16190.1 38.356 73 45 0 2 74 28 100 4.88e-11 56.6
Msa1093220 AT5G16190.2 38.356 73 45 0 2 74 89 161 9.51e-11 55.8

Find 14 sgRNAs with CRISPR-Local

Find 122 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATCACATGGATAATCAATT+TGG 0.303413 7_3:-12748301 None:intergenic
GATCATAGAATGGTTAATTA+AGG 0.317858 7_3:+12748361 Msa1093220:CDS
TCCAGCTTTATATCTGGTCC+TGG 0.428493 7_3:-12748441 None:intergenic
TTACTAATTCAAGTACTTGA+GGG 0.447277 7_3:+12748326 Msa1093220:CDS
TTTACTAATTCAAGTACTTG+AGG 0.502613 7_3:+12748325 Msa1093220:CDS
AGAATGGTTAATTAAGGTTG+AGG 0.508105 7_3:+12748367 Msa1093220:CDS
TCTATCGGCATCGCTTGGCC+AGG 0.517766 7_3:+12748423 Msa1093220:CDS
CATAATCTATCGGCATCGCT+TGG 0.534866 7_3:+12748418 Msa1093220:CDS
GCCAGGACCAGATATAAAGC+TGG 0.559225 7_3:+12748440 Msa1093220:CDS
GGTCTCTAAGTGGAATCTAA+AGG 0.564907 7_3:+12748388 Msa1093220:CDS
AGGCAGTCAACATAATCTAT+CGG 0.575941 7_3:+12748408 Msa1093220:CDS
CTGAAGATGAGATCATAGAA+TGG 0.594946 7_3:+12748351 Msa1093220:CDS
TTAAGGTTGAGGTCTCTAAG+TGG 0.599181 7_3:+12748378 Msa1093220:CDS
TGAATTAGTAAAAGATCACA+TGG 0.639116 7_3:-12748314 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTATAGATATCATTACTTT+AGG + chr7_3:12748072-12748091 Msa1093220:intron 15.0%
!! ATTATAGATATCATTACTTT+AGG + chr7_3:12748072-12748091 Msa1093220:intron 15.0%
!! AATTAATTAACACTATTGCA+CGG - chr7_3:12747911-12747930 None:intergenic 20.0%
!! ATTCATTAAACAACAACAAA+TGG - chr7_3:12748169-12748188 None:intergenic 20.0%
!! AATTAATTAACACTATTGCA+CGG - chr7_3:12747911-12747930 None:intergenic 20.0%
!! ATTCATTAAACAACAACAAA+TGG - chr7_3:12748169-12748188 None:intergenic 20.0%
! TTGAACTGAATTTAAATCGT+TGG + chr7_3:12747538-12747557 Msa1093220:intron 25.0%
!! TTTGTTTCTTATCCAATTTG+TGG + chr7_3:12747673-12747692 Msa1093220:intron 25.0%
! ATATGTATGCATTTATCCAA+TGG + chr7_3:12747785-12747804 Msa1093220:intron 25.0%
! ATAAATGAAACTCAGAAGTT+TGG - chr7_3:12747854-12747873 None:intergenic 25.0%
! TGAGTTTCATTTATTCTAAG+AGG + chr7_3:12747860-12747879 Msa1093220:intron 25.0%
! TTATAAGCAATCAATGACAT+TGG + chr7_3:12748198-12748217 Msa1093220:intron 25.0%
!! TTTGTTCATTTTGCACAATT+TGG + chr7_3:12748240-12748259 Msa1093220:intron 25.0%
! TGAATTAGTAAAAGATCACA+TGG - chr7_3:12748317-12748336 None:intergenic 25.0%
! TTTACTAATTCAAGTACTTG+AGG + chr7_3:12748325-12748344 Msa1093220:CDS 25.0%
! TTACTAATTCAAGTACTTGA+GGG + chr7_3:12748326-12748345 Msa1093220:CDS 25.0%
! GATCATAGAATGGTTAATTA+AGG + chr7_3:12748361-12748380 Msa1093220:CDS 25.0%
! TTGAACTGAATTTAAATCGT+TGG + chr7_3:12747538-12747557 Msa1093220:intron 25.0%
!! TTTGTTTCTTATCCAATTTG+TGG + chr7_3:12747673-12747692 Msa1093220:intron 25.0%
! ATATGTATGCATTTATCCAA+TGG + chr7_3:12747785-12747804 Msa1093220:intron 25.0%
! ATAAATGAAACTCAGAAGTT+TGG - chr7_3:12747854-12747873 None:intergenic 25.0%
! TGAGTTTCATTTATTCTAAG+AGG + chr7_3:12747860-12747879 Msa1093220:intron 25.0%
! TTATAAGCAATCAATGACAT+TGG + chr7_3:12748198-12748217 Msa1093220:intron 25.0%
!! TTTGTTCATTTTGCACAATT+TGG + chr7_3:12748240-12748259 Msa1093220:intron 25.0%
! TGAATTAGTAAAAGATCACA+TGG - chr7_3:12748317-12748336 None:intergenic 25.0%
! TTTACTAATTCAAGTACTTG+AGG + chr7_3:12748325-12748344 Msa1093220:CDS 25.0%
! TTACTAATTCAAGTACTTGA+GGG + chr7_3:12748326-12748345 Msa1093220:CDS 25.0%
! GATCATAGAATGGTTAATTA+AGG + chr7_3:12748361-12748380 Msa1093220:CDS 25.0%
TGAATTTAAATCGTTGGCAT+TGG + chr7_3:12747544-12747563 Msa1093220:intron 30.0%
TCAAAATGGCTTATATTGCT+TGG + chr7_3:12747638-12747657 Msa1093220:intron 30.0%
AATTTAAGAAGGTTAAGCCA+AGG + chr7_3:12747724-12747743 Msa1093220:intron 30.0%
! ATGCATTTATCCAATGGTTT+TGG + chr7_3:12747791-12747810 Msa1093220:intron 30.0%
! TTAATTCAAGCTTTAGCACA+GGG - chr7_3:12748036-12748055 None:intergenic 30.0%
TCAATGACATTGGCAAAATA+TGG + chr7_3:12748208-12748227 Msa1093220:intron 30.0%
GATCACATGGATAATCAATT+TGG - chr7_3:12748304-12748323 None:intergenic 30.0%
AGAATGGTTAATTAAGGTTG+AGG + chr7_3:12748367-12748386 Msa1093220:CDS 30.0%
TGAATTTAAATCGTTGGCAT+TGG + chr7_3:12747544-12747563 Msa1093220:intron 30.0%
TCAAAATGGCTTATATTGCT+TGG + chr7_3:12747638-12747657 Msa1093220:intron 30.0%
AATTTAAGAAGGTTAAGCCA+AGG + chr7_3:12747724-12747743 Msa1093220:intron 30.0%
! ATGCATTTATCCAATGGTTT+TGG + chr7_3:12747791-12747810 Msa1093220:intron 30.0%
! TTAATTCAAGCTTTAGCACA+GGG - chr7_3:12748036-12748055 None:intergenic 30.0%
TCAATGACATTGGCAAAATA+TGG + chr7_3:12748208-12748227 Msa1093220:intron 30.0%
GATCACATGGATAATCAATT+TGG - chr7_3:12748304-12748323 None:intergenic 30.0%
AGAATGGTTAATTAAGGTTG+AGG + chr7_3:12748367-12748386 Msa1093220:CDS 30.0%
!! CATTTTGAAAGACCCTGAAT+GGG - chr7_3:12747626-12747645 None:intergenic 35.0%
! GACTGTATGCTTCTTTGTTT+GGG + chr7_3:12747695-12747714 Msa1093220:intron 35.0%
! ACTGTATGCTTCTTTGTTTG+GGG + chr7_3:12747696-12747715 Msa1093220:intron 35.0%
! TTGGGGTGTCAAATTTAAGA+AGG + chr7_3:12747713-12747732 Msa1093220:intron 35.0%
TCAACATCATCAACCTTGAA+TGG - chr7_3:12747762-12747781 None:intergenic 35.0%
!! TTCAAGGTTGATGATGTTGA+AGG + chr7_3:12747762-12747781 Msa1093220:intron 35.0%
GGAATTTGAACCAAAACCAT+TGG - chr7_3:12747804-12747823 None:intergenic 35.0%
AGGTTGCAGATAATTCTCAA+TGG - chr7_3:12748008-12748027 None:intergenic 35.0%
! GTTAATTCAAGCTTTAGCAC+AGG - chr7_3:12748037-12748056 None:intergenic 35.0%
!! TTGCACAATTTGGCTTTATC+AGG + chr7_3:12748250-12748269 Msa1093220:intron 35.0%
CTGAAGATGAGATCATAGAA+TGG + chr7_3:12748351-12748370 Msa1093220:CDS 35.0%
AGGCAGTCAACATAATCTAT+CGG + chr7_3:12748408-12748427 Msa1093220:CDS 35.0%
!! CAGTTTTCCAGCTTTATATC+TGG - chr7_3:12748450-12748469 None:intergenic 35.0%
!! CATTTTGAAAGACCCTGAAT+GGG - chr7_3:12747626-12747645 None:intergenic 35.0%
! GACTGTATGCTTCTTTGTTT+GGG + chr7_3:12747695-12747714 Msa1093220:intron 35.0%
! ACTGTATGCTTCTTTGTTTG+GGG + chr7_3:12747696-12747715 Msa1093220:intron 35.0%
! TTGGGGTGTCAAATTTAAGA+AGG + chr7_3:12747713-12747732 Msa1093220:intron 35.0%
TCAACATCATCAACCTTGAA+TGG - chr7_3:12747762-12747781 None:intergenic 35.0%
!! TTCAAGGTTGATGATGTTGA+AGG + chr7_3:12747762-12747781 Msa1093220:intron 35.0%
GGAATTTGAACCAAAACCAT+TGG - chr7_3:12747804-12747823 None:intergenic 35.0%
AGGTTGCAGATAATTCTCAA+TGG - chr7_3:12748008-12748027 None:intergenic 35.0%
! GTTAATTCAAGCTTTAGCAC+AGG - chr7_3:12748037-12748056 None:intergenic 35.0%
!! TTGCACAATTTGGCTTTATC+AGG + chr7_3:12748250-12748269 Msa1093220:intron 35.0%
CTGAAGATGAGATCATAGAA+TGG + chr7_3:12748351-12748370 Msa1093220:CDS 35.0%
AGGCAGTCAACATAATCTAT+CGG + chr7_3:12748408-12748427 Msa1093220:CDS 35.0%
!! CAGTTTTCCAGCTTTATATC+TGG - chr7_3:12748450-12748469 None:intergenic 35.0%
TTGGTTCAGAAAATTCTCGC+TGG + chr7_3:12747563-12747582 Msa1093220:intron 40.0%
TTCAGAAAATTCTCGCTGGA+GGG + chr7_3:12747567-12747586 Msa1093220:intron 40.0%
!! CCATTTTGAAAGACCCTGAA+TGG - chr7_3:12747627-12747646 None:intergenic 40.0%
CCATTCAGGGTCTTTCAAAA+TGG + chr7_3:12747624-12747643 Msa1093220:intron 40.0%
GAAGCATACAGTCCACAAAT+TGG - chr7_3:12747688-12747707 None:intergenic 40.0%
GGACTGTATGCTTCTTTGTT+TGG + chr7_3:12747694-12747713 Msa1093220:intron 40.0%
AGGTTAAGCCAAGGATTAAC+AGG + chr7_3:12747733-12747752 Msa1093220:intron 40.0%
GGTTAAGCCAAGGATTAACA+GGG + chr7_3:12747734-12747753 Msa1093220:intron 40.0%
AATGGATCCCTGTTAATCCT+TGG - chr7_3:12747744-12747763 None:intergenic 40.0%
GATTAACAGGGATCCATTCA+AGG + chr7_3:12747746-12747765 Msa1093220:intron 40.0%
TCCAATGTGCAATGAGAAAG+AGG + chr7_3:12747821-12747840 Msa1093220:intron 40.0%
ACCTCTTTCTCATTGCACAT+TGG - chr7_3:12747825-12747844 None:intergenic 40.0%
TTAAGGTTGAGGTCTCTAAG+TGG + chr7_3:12748378-12748397 Msa1093220:CDS 40.0%
GGTCTCTAAGTGGAATCTAA+AGG + chr7_3:12748388-12748407 Msa1093220:CDS 40.0%
TTGGTTCAGAAAATTCTCGC+TGG + chr7_3:12747563-12747582 Msa1093220:intron 40.0%
TTCAGAAAATTCTCGCTGGA+GGG + chr7_3:12747567-12747586 Msa1093220:intron 40.0%
!! CCATTTTGAAAGACCCTGAA+TGG - chr7_3:12747627-12747646 None:intergenic 40.0%
CCATTCAGGGTCTTTCAAAA+TGG + chr7_3:12747624-12747643 Msa1093220:intron 40.0%
GAAGCATACAGTCCACAAAT+TGG - chr7_3:12747688-12747707 None:intergenic 40.0%
GGACTGTATGCTTCTTTGTT+TGG + chr7_3:12747694-12747713 Msa1093220:intron 40.0%
AGGTTAAGCCAAGGATTAAC+AGG + chr7_3:12747733-12747752 Msa1093220:intron 40.0%
GGTTAAGCCAAGGATTAACA+GGG + chr7_3:12747734-12747753 Msa1093220:intron 40.0%
AATGGATCCCTGTTAATCCT+TGG - chr7_3:12747744-12747763 None:intergenic 40.0%
GATTAACAGGGATCCATTCA+AGG + chr7_3:12747746-12747765 Msa1093220:intron 40.0%
TCCAATGTGCAATGAGAAAG+AGG + chr7_3:12747821-12747840 Msa1093220:intron 40.0%
ACCTCTTTCTCATTGCACAT+TGG - chr7_3:12747825-12747844 None:intergenic 40.0%
TTAAGGTTGAGGTCTCTAAG+TGG + chr7_3:12748378-12748397 Msa1093220:CDS 40.0%
GGTCTCTAAGTGGAATCTAA+AGG + chr7_3:12748388-12748407 Msa1093220:CDS 40.0%
GTTCAGAAAATTCTCGCTGG+AGG + chr7_3:12747566-12747585 Msa1093220:intron 45.0%
TACATTGCTCCACCCATTCA+GGG + chr7_3:12747611-12747630 Msa1093220:intron 45.0%
TTTGAAAGACCCTGAATGGG+TGG - chr7_3:12747623-12747642 None:intergenic 45.0%
ACTATTGCACGGAAACTTGC+TGG - chr7_3:12747900-12747919 None:intergenic 45.0%
GATTTCACAGATGTCGCAAG+AGG + chr7_3:12747948-12747967 Msa1093220:intron 45.0%
! AGCTTTAGCACAGGGAATAG+AGG - chr7_3:12748028-12748047 None:intergenic 45.0%
CATAATCTATCGGCATCGCT+TGG + chr7_3:12748418-12748437 Msa1093220:CDS 45.0%
! TCCAGCTTTATATCTGGTCC+TGG - chr7_3:12748444-12748463 None:intergenic 45.0%
GTTCAGAAAATTCTCGCTGG+AGG + chr7_3:12747566-12747585 Msa1093220:intron 45.0%
TACATTGCTCCACCCATTCA+GGG + chr7_3:12747611-12747630 Msa1093220:intron 45.0%
TTTGAAAGACCCTGAATGGG+TGG - chr7_3:12747623-12747642 None:intergenic 45.0%
ACTATTGCACGGAAACTTGC+TGG - chr7_3:12747900-12747919 None:intergenic 45.0%
GATTTCACAGATGTCGCAAG+AGG + chr7_3:12747948-12747967 Msa1093220:intron 45.0%
! AGCTTTAGCACAGGGAATAG+AGG - chr7_3:12748028-12748047 None:intergenic 45.0%
CATAATCTATCGGCATCGCT+TGG + chr7_3:12748418-12748437 Msa1093220:CDS 45.0%
! TCCAGCTTTATATCTGGTCC+TGG - chr7_3:12748444-12748463 None:intergenic 45.0%
CTACATTGCTCCACCCATTC+AGG + chr7_3:12747610-12747629 Msa1093220:intron 50.0%
GCTTTATCAGGTGTAGCAGC+AGG + chr7_3:12748262-12748281 Msa1093220:intron 50.0%
GCCAGGACCAGATATAAAGC+TGG + chr7_3:12748440-12748459 Msa1093220:CDS 50.0%
CTACATTGCTCCACCCATTC+AGG + chr7_3:12747610-12747629 Msa1093220:intron 50.0%
GCTTTATCAGGTGTAGCAGC+AGG + chr7_3:12748262-12748281 Msa1093220:intron 50.0%
GCCAGGACCAGATATAAAGC+TGG + chr7_3:12748440-12748459 Msa1093220:CDS 50.0%
TCTATCGGCATCGCTTGGCC+AGG + chr7_3:12748423-12748442 Msa1093220:CDS 60.0%
TCTATCGGCATCGCTTGGCC+AGG + chr7_3:12748423-12748442 Msa1093220:CDS 60.0%
Chromosome Type Strat End Strand Name
chr7_3 gene 12747475 12748493 12747475 ID=Msa1093220;Name=Msa1093220
chr7_3 mRNA 12747475 12748493 12747475 ID=Msa1093220-mRNA-1;Parent=Msa1093220;Name=Msa1093220-mRNA-1;_AED=0.46;_eAED=0.47;_QI=0|0|0|1|0|0|2|0|74
chr7_3 exon 12747475 12747480 12747475 ID=Msa1093220-mRNA-1:exon:3011;Parent=Msa1093220-mRNA-1
chr7_3 exon 12748278 12748493 12748278 ID=Msa1093220-mRNA-1:exon:3012;Parent=Msa1093220-mRNA-1
chr7_3 CDS 12747475 12747480 12747475 ID=Msa1093220-mRNA-1:cds;Parent=Msa1093220-mRNA-1
chr7_3 CDS 12748278 12748493 12748278 ID=Msa1093220-mRNA-1:cds;Parent=Msa1093220-mRNA-1
Gene Sequence

>Msa1093220

GCTTATCAGCAGGCAATTTATGCAGTCTGCCAAATTGATTATCCATGTGATCTTTTACTAATTCAAGTACTTGAGGGTTCTGAAGATGAGATCATAGAATGGTTAATTAAGGTTGAGGTCTCTAAGTGGAATCTAAAGGCAGTCAACATAATCTATCGGCATCGCTTGGCCAGGACCAGATATAAAGCTGGAAAACTGAATTGTGCAATGAGATGTGACTAT

Protein sequence

>Msa1093220

AYQQAIYAVCQIDYPCDLLLIQVLEGSEDEIIEWLIKVEVSKWNLKAVNIIYRHRLARTRYKAGKLNCAMRCDY