Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1095450 | XP_013447886.1 | 94.161 | 137 | 7 | 1 | 1 | 136 | 1 | 137 | 5.55e-87 | 259 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1095450 | A0A072TYJ6 | 94.161 | 137 | 7 | 1 | 1 | 136 | 1 | 137 | 2.65e-87 | 259 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa1011850 | Msa1095450 | 0.808801 | 2.590853e-50 | -8.615850e-47 |
| Msa1053300 | Msa1095450 | 0.825500 | 4.531832e-54 | -8.615850e-47 |
| Msa1095450 | Msa1142590 | 0.802766 | 4.800580e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1095450 | MtrunA17_Chr7g0223391 | 94.161 | 137 | 7 | 1 | 1 | 136 | 18 | 154 | 9.57e-91 | 259 |
| Msa1095450 | MtrunA17_Chr7g0223401 | 89.781 | 137 | 13 | 1 | 1 | 136 | 6 | 142 | 5.70e-86 | 247 |
| Msa1095450 | MtrunA17_Chr8g0356181 | 48.571 | 140 | 68 | 2 | 1 | 136 | 1 | 140 | 1.16e-40 | 132 |
| Msa1095450 | MtrunA17_Chr1g0190921 | 36.641 | 131 | 77 | 2 | 1 | 130 | 1 | 126 | 3.13e-19 | 77.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1095450 | AT2G44310.1 | 46.715 | 137 | 70 | 2 | 1 | 134 | 1 | 137 | 5.80e-38 | 126 |
Find 24 sgRNAs with CRISPR-Local
Find 27 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAAACACGAGCAAGCTCATT+TGG | 0.308059 | 7_3:-18311797 | None:intergenic |
| CTTGAATGGCACGGTTGATC+TGG | 0.357110 | 7_3:+18311850 | Msa1095450:CDS |
| TGAAAGAGAGACCACTAAAT+TGG | 0.360423 | 7_3:+18311982 | Msa1095450:CDS |
| TATTAAATGCTTCATCTTCA+AGG | 0.376744 | 7_3:-18311643 | None:intergenic |
| CATGCCAATTCAAATGGTCT+TGG | 0.391639 | 7_3:+18311931 | Msa1095450:CDS |
| TCTTCCAAGACCATTTGAAT+TGG | 0.395041 | 7_3:-18311935 | None:intergenic |
| TATTTAAGCAGCCAATTTAG+TGG | 0.401864 | 7_3:-18311993 | None:intergenic |
| TTAATAGCTCAGTTGGAAAT+CGG | 0.432475 | 7_3:+18311660 | Msa1095450:CDS |
| GAAGCATTTAATAGCTCAGT+TGG | 0.438228 | 7_3:+18311653 | Msa1095450:CDS |
| ATGCTTCATCTTCAAGGAAG+TGG | 0.440600 | 7_3:-18311637 | None:intergenic |
| CTCCTTCAACATCTCATCAT+AGG | 0.454389 | 7_3:-18311720 | None:intergenic |
| AAACACGAGCAAGCTCATTT+GGG | 0.456333 | 7_3:-18311796 | None:intergenic |
| GGGTTTCATGCCAATTCAAA+TGG | 0.458206 | 7_3:+18311925 | Msa1095450:CDS |
| CTCCTATGATGAGATGTTGA+AGG | 0.467641 | 7_3:+18311718 | Msa1095450:CDS |
| CAGTTCGATCACAACTTGAA+TGG | 0.477280 | 7_3:+18311836 | Msa1095450:CDS |
| GAAGGAGCTCAAGTGCTTGA+GGG | 0.496779 | 7_3:+18311736 | Msa1095450:CDS |
| GCTTGCAATGGCAGATGGAA+TGG | 0.514699 | 7_3:+18311904 | Msa1095450:CDS |
| CTTGCAATGGCAGATGGAAT+GGG | 0.535718 | 7_3:+18311905 | Msa1095450:CDS |
| TGAAGGAGCTCAAGTGCTTG+AGG | 0.550425 | 7_3:+18311735 | Msa1095450:CDS |
| ATGATGCTTGCAATGGCAGA+TGG | 0.563021 | 7_3:+18311899 | Msa1095450:CDS |
| TTCAAGGAAGTGGACAATTG+TGG | 0.596143 | 7_3:-18311627 | None:intergenic |
| TAAGCAAATGATGCTTGCAA+TGG | 0.606338 | 7_3:+18311892 | Msa1095450:CDS |
| CGATCACAACTTGAATGGCA+CGG | 0.606932 | 7_3:+18311841 | Msa1095450:CDS |
| TGCCTCGACACAAATAACGA+CGG | 0.718573 | 7_3:+18311689 | Msa1095450:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATGAGTGTAGAAATTTTAGA+TGG | + | chr7_3:18311602-18311621 | Msa1095450:CDS | 25.0% |
| ! | TATTAAATGCTTCATCTTCA+AGG | - | chr7_3:18311646-18311665 | None:intergenic | 25.0% |
| TTAATAGCTCAGTTGGAAAT+CGG | + | chr7_3:18311660-18311679 | Msa1095450:CDS | 30.0% | |
| !! | CTCTTTCAACAGCTATTTTA+AGG | - | chr7_3:18311970-18311989 | None:intergenic | 30.0% |
| ! | GAAGCATTTAATAGCTCAGT+TGG | + | chr7_3:18311653-18311672 | Msa1095450:CDS | 35.0% |
| !!! | AGGGTTTTTGAAACACACTT+TGG | + | chr7_3:18311755-18311774 | Msa1095450:CDS | 35.0% |
| TAAGCAAATGATGCTTGCAA+TGG | + | chr7_3:18311892-18311911 | Msa1095450:CDS | 35.0% | |
| TCTTCCAAGACCATTTGAAT+TGG | - | chr7_3:18311938-18311957 | None:intergenic | 35.0% | |
| TGAAAGAGAGACCACTAAAT+TGG | + | chr7_3:18311982-18312001 | Msa1095450:CDS | 35.0% | |
| TTCAAGGAAGTGGACAATTG+TGG | - | chr7_3:18311630-18311649 | None:intergenic | 40.0% | |
| ATGCTTCATCTTCAAGGAAG+TGG | - | chr7_3:18311640-18311659 | None:intergenic | 40.0% | |
| CTCCTATGATGAGATGTTGA+AGG | + | chr7_3:18311718-18311737 | Msa1095450:CDS | 40.0% | |
| CTCCTTCAACATCTCATCAT+AGG | - | chr7_3:18311723-18311742 | None:intergenic | 40.0% | |
| AAACACGAGCAAGCTCATTT+GGG | - | chr7_3:18311799-18311818 | None:intergenic | 40.0% | |
| TAAACACGAGCAAGCTCATT+TGG | - | chr7_3:18311800-18311819 | None:intergenic | 40.0% | |
| CAGTTCGATCACAACTTGAA+TGG | + | chr7_3:18311836-18311855 | Msa1095450:CDS | 40.0% | |
| GGGTTTCATGCCAATTCAAA+TGG | + | chr7_3:18311925-18311944 | Msa1095450:CDS | 40.0% | |
| CATGCCAATTCAAATGGTCT+TGG | + | chr7_3:18311931-18311950 | Msa1095450:CDS | 40.0% | |
| TGCCTCGACACAAATAACGA+CGG | + | chr7_3:18311689-18311708 | Msa1095450:CDS | 45.0% | |
| CGATCACAACTTGAATGGCA+CGG | + | chr7_3:18311841-18311860 | Msa1095450:CDS | 45.0% | |
| ATGATGCTTGCAATGGCAGA+TGG | + | chr7_3:18311899-18311918 | Msa1095450:CDS | 45.0% | |
| CTTGCAATGGCAGATGGAAT+GGG | + | chr7_3:18311905-18311924 | Msa1095450:CDS | 45.0% | |
| GACCGTCGTTATTTGTGTCG+AGG | - | chr7_3:18311694-18311713 | None:intergenic | 50.0% | |
| TGAAGGAGCTCAAGTGCTTG+AGG | + | chr7_3:18311735-18311754 | Msa1095450:CDS | 50.0% | |
| GAAGGAGCTCAAGTGCTTGA+GGG | + | chr7_3:18311736-18311755 | Msa1095450:CDS | 50.0% | |
| CTTGAATGGCACGGTTGATC+TGG | + | chr7_3:18311850-18311869 | Msa1095450:CDS | 50.0% | |
| GCTTGCAATGGCAGATGGAA+TGG | + | chr7_3:18311904-18311923 | Msa1095450:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_3 | gene | 18311602 | 18312012 | 18311602 | ID=Msa1095450;Name=Msa1095450 |
| chr7_3 | mRNA | 18311602 | 18312012 | 18311602 | ID=Msa1095450-mRNA-1;Parent=Msa1095450;Name=Msa1095450-mRNA-1;_AED=0.38;_eAED=0.38;_QI=0|-1|0|1|-1|1|1|0|136 |
| chr7_3 | exon | 18311602 | 18312012 | 18311602 | ID=Msa1095450-mRNA-1:exon:3970;Parent=Msa1095450-mRNA-1 |
| chr7_3 | CDS | 18311602 | 18312012 | 18311602 | ID=Msa1095450-mRNA-1:cds;Parent=Msa1095450-mRNA-1 |
| Gene Sequence |
| Protein sequence |
