Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1142590 | XP_013447886.1 | 95.620 | 137 | 6 | 0 | 1 | 137 | 1 | 137 | 3.74e-90 | 268 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1142590 | A0A072TYJ6 | 95.620 | 137 | 6 | 0 | 1 | 137 | 1 | 137 | 1.79e-90 | 268 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0034100 | Msa1142590 | -0.820750 | 5.816695e-53 | -8.615850e-47 |
| Msa0040360 | Msa1142590 | 0.806250 | 9.010306e-50 | -8.615850e-47 |
| Msa0051910 | Msa1142590 | 0.805165 | 1.522546e-49 | -8.615850e-47 |
| Msa0054790 | Msa1142590 | 0.805937 | 1.048985e-49 | -8.615850e-47 |
| Msa0072440 | Msa1142590 | 0.805574 | 1.249814e-49 | -8.615850e-47 |
| Msa0101530 | Msa1142590 | 0.810659 | 1.032642e-50 | -8.615850e-47 |
| Msa0603310 | Msa1142590 | 0.804361 | 2.241657e-49 | -8.615850e-47 |
| Msa0606600 | Msa1142590 | 0.829950 | 3.864274e-55 | -8.615850e-47 |
| Msa0610240 | Msa1142590 | -0.805690 | 1.182009e-49 | -8.615850e-47 |
| Msa0630180 | Msa1142590 | 0.807159 | 5.792565e-50 | -8.615850e-47 |
| Msa0647300 | Msa1142590 | 0.828247 | 9.995455e-55 | -8.615850e-47 |
| Msa0659020 | Msa1142590 | -0.802765 | 4.803091e-49 | -8.615850e-47 |
| Msa0685390 | Msa1142590 | -0.812488 | 4.134342e-51 | -8.615850e-47 |
| Msa0686830 | Msa1142590 | 0.832112 | 1.138174e-55 | -8.615850e-47 |
| Msa0703430 | Msa1142590 | -0.805979 | 1.027673e-49 | -8.615850e-47 |
| Msa0708680 | Msa1142590 | 0.804413 | 2.186169e-49 | -8.615850e-47 |
| Msa0721930 | Msa1142590 | 0.803970 | 2.703452e-49 | -8.615850e-47 |
| Msa0731580 | Msa1142590 | 0.817006 | 4.123614e-52 | -8.615850e-47 |
| Msa0756670 | Msa1142590 | -0.810360 | 1.198397e-50 | -8.615850e-47 |
| Msa0762930 | Msa1142590 | 0.803848 | 2.865632e-49 | -8.615850e-47 |
| Msa0809920 | Msa1142590 | 0.816129 | 6.482208e-52 | -8.615850e-47 |
| Msa0819150 | Msa1142590 | 0.800693 | 1.278584e-48 | -8.615850e-47 |
| Msa0836060 | Msa1142590 | -0.801947 | 7.079544e-49 | -8.615850e-47 |
| Msa0120860 | Msa1142590 | 0.831710 | 1.430185e-55 | -8.615850e-47 |
| Msa0121320 | Msa1142590 | 0.809471 | 1.861653e-50 | -8.615850e-47 |
| Msa0128110 | Msa1142590 | -0.808361 | 3.216712e-50 | -8.615850e-47 |
| Msa0128340 | Msa1142590 | 0.810252 | 1.264447e-50 | -8.615850e-47 |
| Msa0128740 | Msa1142590 | 0.803033 | 4.228735e-49 | -8.615850e-47 |
| Msa0136180 | Msa1142590 | -0.811560 | 6.587991e-51 | -8.615850e-47 |
| Msa0166180 | Msa1142590 | 0.812835 | 3.471419e-51 | -8.615850e-47 |
| Msa0180210 | Msa1142590 | 0.804508 | 2.088545e-49 | -8.615850e-47 |
| Msa0186200 | Msa1142590 | 0.815511 | 8.906023e-52 | -8.615850e-47 |
| Msa0193680 | Msa1142590 | 0.807650 | 4.556015e-50 | -8.615850e-47 |
| Msa0214800 | Msa1142590 | 0.808703 | 2.719200e-50 | -8.615850e-47 |
| Msa0373550 | Msa1142590 | 0.812612 | 3.883567e-51 | -8.615850e-47 |
| Msa0383770 | Msa1142590 | 0.804454 | 2.144088e-49 | -8.615850e-47 |
| Msa0384590 | Msa1142590 | 0.808749 | 2.657331e-50 | -8.615850e-47 |
| Msa0468660 | Msa1142590 | 0.813688 | 2.255430e-51 | -8.615850e-47 |
| Msa1011800 | Msa1142590 | 0.926750 | 3.050819e-91 | -8.615850e-47 |
| Msa1011820 | Msa1142590 | 0.826860 | 2.150869e-54 | -8.615850e-47 |
| Msa1011840 | Msa1142590 | 0.825904 | 3.634259e-54 | -8.615850e-47 |
| Msa1011860 | Msa1142590 | 0.831358 | 1.746555e-55 | -8.615850e-47 |
| Msa1011870 | Msa1142590 | 0.880262 | 6.343080e-70 | -8.615850e-47 |
| Msa1030320 | Msa1142590 | 0.807774 | 4.288473e-50 | -8.615850e-47 |
| Msa1053300 | Msa1142590 | 0.824665 | 7.137606e-54 | -8.615850e-47 |
| Msa1053310 | Msa1142590 | 0.946170 | 7.687067e-105 | -8.615850e-47 |
| Msa1053330 | Msa1142590 | 0.856700 | 2.694203e-62 | -8.615850e-47 |
| Msa1053340 | Msa1142590 | 0.983159 | 5.470362e-157 | -8.615850e-47 |
| Msa1088400 | Msa1142590 | -0.822256 | 2.610214e-53 | -8.615850e-47 |
| Msa1093190 | Msa1142590 | -0.800176 | 1.629407e-48 | -8.615850e-47 |
| Msa1094810 | Msa1142590 | 0.802888 | 4.530234e-49 | -8.615850e-47 |
| Msa1095400 | Msa1142590 | 0.845002 | 5.326512e-59 | -8.615850e-47 |
| Msa1095410 | Msa1142590 | 0.957021 | 7.398520e-115 | -8.615850e-47 |
| Msa1095440 | Msa1142590 | 0.805395 | 1.362816e-49 | -8.615850e-47 |
| Msa1095450 | Msa1142590 | 0.802766 | 4.800580e-49 | -8.615850e-47 |
| Msa0242720 | Msa1142590 | 0.831550 | 1.566607e-55 | -8.615850e-47 |
| Msa0274570 | Msa1142590 | 0.804604 | 1.994274e-49 | -8.615850e-47 |
| Msa0294200 | Msa1142590 | 0.811774 | 5.915936e-51 | -8.615850e-47 |
| Msa0348370 | Msa1142590 | 0.800544 | 1.371172e-48 | -8.615850e-47 |
| Msa1107330 | Msa1142590 | 0.806659 | 7.388986e-50 | -8.615850e-47 |
| Msa1142570 | Msa1142590 | 0.827982 | 1.158157e-54 | -8.615850e-47 |
| Msa1142580 | Msa1142590 | 0.845259 | 4.540373e-59 | -8.615850e-47 |
| Msa1142590 | Msa1142600 | 0.832201 | 1.082329e-55 | -8.615850e-47 |
| Msa1142590 | Msa1146980 | 0.835791 | 1.366300e-56 | -8.615850e-47 |
| Msa1142590 | Msa1157210 | 0.822418 | 2.393925e-53 | -8.615850e-47 |
| Msa1142590 | Msa1165460 | 0.808637 | 2.808917e-50 | -8.615850e-47 |
| Msa1142590 | Msa1186670 | 0.810791 | 9.669920e-51 | -8.615850e-47 |
| Msa1142590 | Msa1191350 | 0.800652 | 1.303385e-48 | -8.615850e-47 |
| Msa1142590 | Msa1197720 | 0.845589 | 3.696832e-59 | -8.615850e-47 |
| Msa1142590 | Msa1211130 | 0.812678 | 3.756668e-51 | -8.615850e-47 |
| Msa1142590 | Msa1251410 | 0.821541 | 3.822057e-53 | -8.615850e-47 |
| Msa1142590 | Msa1263110 | 0.800115 | 1.676512e-48 | -8.615850e-47 |
| Msa1142590 | Msa1319190 | 0.802070 | 6.677718e-49 | -8.615850e-47 |
| Msa1142590 | Msa1322500 | 0.809039 | 2.303954e-50 | -8.615850e-47 |
| Msa1142590 | Msa1359590 | 0.811372 | 7.236262e-51 | -8.615850e-47 |
| Msa1142590 | Msa1361980 | 0.810234 | 1.275512e-50 | -8.615850e-47 |
| Msa1142590 | Msa1400290 | -0.827035 | 1.953300e-54 | -8.615850e-47 |
| Msa1142590 | Msa1403660 | 0.814511 | 1.484182e-51 | -8.615850e-47 |
| Msa1142590 | Msa1429810 | 0.824987 | 5.991604e-54 | -8.615850e-47 |
| Msa1142590 | Msa1455360 | 0.807716 | 4.412533e-50 | -8.615850e-47 |
| Msa1142590 | Msa1462780 | 0.829214 | 5.832789e-55 | -8.615850e-47 |
| Msa0491780 | Msa1142590 | 0.800408 | 1.461857e-48 | -8.615850e-47 |
| Msa0514540 | Msa1142590 | 0.803119 | 4.058785e-49 | -8.615850e-47 |
| Msa0522280 | Msa1142590 | 0.811939 | 5.448643e-51 | -8.615850e-47 |
| Msa0850480 | Msa1142590 | 0.812400 | 4.321012e-51 | -8.615850e-47 |
| Msa0882370 | Msa1142590 | 0.814321 | 1.635459e-51 | -8.615850e-47 |
| Msa0900830 | Msa1142590 | 0.824851 | 6.450792e-54 | -8.615850e-47 |
| Msa0921860 | Msa1142590 | 0.803281 | 3.756626e-49 | -8.615850e-47 |
| Msa0926240 | Msa1142590 | 0.838676 | 2.496227e-57 | -8.615850e-47 |
| Msa0932140 | Msa1142590 | 0.822845 | 1.905029e-53 | -8.615850e-47 |
| Msa0932150 | Msa1142590 | 0.834046 | 3.757529e-56 | -8.615850e-47 |
| Msa0936630 | Msa1142590 | 0.809903 | 1.503135e-50 | -8.615850e-47 |
| Msa0965800 | Msa1142590 | 0.837390 | 5.347153e-57 | -8.615850e-47 |
| Msa0970270 | Msa1142590 | 0.818385 | 2.015301e-52 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1142590 | MtrunA17_Chr7g0223391 | 95.620 | 137 | 6 | 0 | 1 | 137 | 18 | 154 | 7.63e-94 | 267 |
| Msa1142590 | MtrunA17_Chr7g0223401 | 94.891 | 137 | 7 | 0 | 1 | 137 | 6 | 142 | 1.38e-92 | 264 |
| Msa1142590 | MtrunA17_Chr8g0356181 | 47.857 | 140 | 70 | 1 | 1 | 137 | 1 | 140 | 8.69e-41 | 132 |
| Msa1142590 | MtrunA17_Chr1g0190921 | 36.641 | 131 | 78 | 1 | 1 | 131 | 1 | 126 | 5.40e-20 | 79.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1142590 | AT2G44310.1 | 46.715 | 137 | 71 | 1 | 1 | 135 | 1 | 137 | 9.62e-40 | 130 |
Find 26 sgRNAs with CRISPR-Local
Find 29 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGATGAGTGTAGAAATTT+TGG | 0.159822 | 7_4:-17104632 | None:intergenic |
| CTTGAATGGCACAATTGATT+TGG | 0.268459 | 7_4:-17104377 | Msa1142590:CDS |
| TAAACACGAGCAAGCTCGTT+TGG | 0.337682 | 7_4:+17104430 | None:intergenic |
| TAAATGCTTCTTCATCTTCA+AGG | 0.379890 | 7_4:+17104587 | None:intergenic |
| CATGCCAATTCAAATGGTCT+TGG | 0.391639 | 7_4:-17104296 | Msa1142590:CDS |
| TCTTCCAAGACCATTTGAAT+TGG | 0.395041 | 7_4:+17104292 | None:intergenic |
| GAAGCATTTAATAGCTCAGT+TGG | 0.431408 | 7_4:-17104574 | Msa1142590:CDS |
| TTAATAGCTCAGTTGGAAAT+CGG | 0.432475 | 7_4:-17104567 | Msa1142590:CDS |
| CTTCTTCATCTTCAAGGAAG+TGG | 0.447415 | 7_4:+17104593 | None:intergenic |
| GGGTTTCATGCCAATTCAAA+TGG | 0.458206 | 7_4:-17104302 | Msa1142590:CDS |
| AAACACGAGCAAGCTCGTTT+GGG | 0.462526 | 7_4:+17104431 | None:intergenic |
| GAAGGAGCTCCGGAGCTTGA+GGG | 0.481338 | 7_4:-17104491 | Msa1142590:CDS |
| CAGTTCGATCACAACTTGAA+TGG | 0.485615 | 7_4:-17104391 | Msa1142590:CDS |
| CTCTTATGATGAGATGTTGA+AGG | 0.511648 | 7_4:-17104509 | Msa1142590:CDS |
| GCTTGCAATGGCAGATGGGA+TGG | 0.521128 | 7_4:-17104323 | Msa1142590:CDS |
| TGAAGGAGCTCCGGAGCTTG+AGG | 0.522904 | 7_4:-17104492 | Msa1142590:CDS |
| ATGAGATGTTGAAGGAGCTC+CGG | 0.525969 | 7_4:-17104501 | Msa1142590:CDS |
| TGAAAGAGAGTACAATAAAG+TGG | 0.551009 | 7_4:-17104245 | Msa1142590:CDS |
| CTTGCAATGGCAGATGGGAT+GGG | 0.557101 | 7_4:-17104322 | Msa1142590:CDS |
| ATGATGCTTGCAATGGCAGA+TGG | 0.558215 | 7_4:-17104328 | Msa1142590:CDS |
| AACACACTTTGGTATTGATG+TGG | 0.581840 | 7_4:-17104461 | Msa1142590:CDS |
| TTCAAGGAAGTGGACAATTG+TGG | 0.596143 | 7_4:+17104603 | None:intergenic |
| CGCCTCGACACAAACAGCGA+TGG | 0.598011 | 7_4:-17104538 | Msa1142590:CDS |
| AAAGCAGATGATGCTTGCAA+TGG | 0.609930 | 7_4:-17104335 | Msa1142590:CDS |
| GACCATCGCTGTTTGTGTCG+AGG | 0.621315 | 7_4:+17104536 | None:intergenic |
| TGATGCTTGCAATGGCAGAT+GGG | 0.625156 | 7_4:-17104327 | Msa1142590:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATGAGTGTAGAAATTTTGGA+TGG | - | chr7_4:17104237-17104256 | Msa1142590:CDS | 30.0% |
| TAAATGCTTCTTCATCTTCA+AGG | + | chr7_4:17104281-17104300 | None:intergenic | 30.0% | |
| TTAATAGCTCAGTTGGAAAT+CGG | - | chr7_4:17104298-17104317 | Msa1142590:CDS | 30.0% | |
| ! | TGAAAGAGAGTACAATAAAG+TGG | - | chr7_4:17104620-17104639 | Msa1142590:CDS | 30.0% |
| ! | GAAGCATTTAATAGCTCAGT+TGG | - | chr7_4:17104291-17104310 | Msa1142590:CDS | 35.0% |
| CTCTTATGATGAGATGTTGA+AGG | - | chr7_4:17104356-17104375 | Msa1142590:CDS | 35.0% | |
| !!! | AGGGTTTTTGAAACACACTT+TGG | - | chr7_4:17104393-17104412 | Msa1142590:CDS | 35.0% |
| !! | AACACACTTTGGTATTGATG+TGG | - | chr7_4:17104404-17104423 | Msa1142590:CDS | 35.0% |
| CTTGAATGGCACAATTGATT+TGG | - | chr7_4:17104488-17104507 | Msa1142590:CDS | 35.0% | |
| TCTTCCAAGACCATTTGAAT+TGG | + | chr7_4:17104576-17104595 | None:intergenic | 35.0% | |
| !! | CTCTTTCAACAGCTATTTTG+AGG | + | chr7_4:17104608-17104627 | None:intergenic | 35.0% |
| TTCAAGGAAGTGGACAATTG+TGG | + | chr7_4:17104265-17104284 | None:intergenic | 40.0% | |
| CTTCTTCATCTTCAAGGAAG+TGG | + | chr7_4:17104275-17104294 | None:intergenic | 40.0% | |
| TTTCAAAAACCCTCAAGCTC+CGG | + | chr7_4:17104386-17104405 | None:intergenic | 40.0% | |
| CAGTTCGATCACAACTTGAA+TGG | - | chr7_4:17104474-17104493 | Msa1142590:CDS | 40.0% | |
| AAAGCAGATGATGCTTGCAA+TGG | - | chr7_4:17104530-17104549 | Msa1142590:CDS | 40.0% | |
| GGGTTTCATGCCAATTCAAA+TGG | - | chr7_4:17104563-17104582 | Msa1142590:CDS | 40.0% | |
| CATGCCAATTCAAATGGTCT+TGG | - | chr7_4:17104569-17104588 | Msa1142590:CDS | 40.0% | |
| ATGAGATGTTGAAGGAGCTC+CGG | - | chr7_4:17104364-17104383 | Msa1142590:CDS | 45.0% | |
| AAACACGAGCAAGCTCGTTT+GGG | + | chr7_4:17104437-17104456 | None:intergenic | 45.0% | |
| TAAACACGAGCAAGCTCGTT+TGG | + | chr7_4:17104438-17104457 | None:intergenic | 45.0% | |
| ATGATGCTTGCAATGGCAGA+TGG | - | chr7_4:17104537-17104556 | Msa1142590:CDS | 45.0% | |
| TGATGCTTGCAATGGCAGAT+GGG | - | chr7_4:17104538-17104557 | Msa1142590:CDS | 45.0% | |
| CTTGCAATGGCAGATGGGAT+GGG | - | chr7_4:17104543-17104562 | Msa1142590:CDS | 50.0% | |
| GACCATCGCTGTTTGTGTCG+AGG | + | chr7_4:17104332-17104351 | None:intergenic | 55.0% | |
| GCTTGCAATGGCAGATGGGA+TGG | - | chr7_4:17104542-17104561 | Msa1142590:CDS | 55.0% | |
| CGCCTCGACACAAACAGCGA+TGG | - | chr7_4:17104327-17104346 | Msa1142590:CDS | 60.0% | |
| TGAAGGAGCTCCGGAGCTTG+AGG | - | chr7_4:17104373-17104392 | Msa1142590:CDS | 60.0% | |
| GAAGGAGCTCCGGAGCTTGA+GGG | - | chr7_4:17104374-17104393 | Msa1142590:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_4 | gene | 17104237 | 17104650 | 17104237 | ID=Msa1142590;Name=Msa1142590 |
| chr7_4 | mRNA | 17104237 | 17104650 | 17104237 | ID=Msa1142590-mRNA-1;Parent=Msa1142590;Name=Msa1142590-mRNA-1;_AED=0.40;_eAED=0.40;_QI=0|-1|0|1|-1|1|1|0|137 |
| chr7_4 | exon | 17104237 | 17104650 | 17104237 | ID=Msa1142590-mRNA-1:exon:3836;Parent=Msa1142590-mRNA-1 |
| chr7_4 | CDS | 17104237 | 17104650 | 17104237 | ID=Msa1142590-mRNA-1:cds;Parent=Msa1142590-mRNA-1 |
| Gene Sequence |
| Protein sequence |
