Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1011850 | XP_013447886.1 | 94.161 | 137 | 7 | 1 | 1 | 136 | 1 | 137 | 1.68e-86 | 258 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1011850 | A0A072TYJ6 | 94.161 | 137 | 7 | 1 | 1 | 136 | 1 | 137 | 8.03e-87 | 258 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa1011810 | Msa1011850 | 0.875337 | 3.337328e-68 | -8.615850e-47 |
| Msa1011830 | Msa1011850 | 0.877419 | 6.381238e-69 | -8.615850e-47 |
| Msa1011850 | Msa1011860 | 0.805759 | 1.142866e-49 | -8.615850e-47 |
| Msa1011850 | Msa1053300 | 0.840298 | 9.460960e-58 | -8.615850e-47 |
| Msa1011850 | Msa1053320 | 0.853877 | 1.787174e-61 | -8.615850e-47 |
| Msa1011850 | Msa1095450 | 0.808801 | 2.590853e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1011850 | MtrunA17_Chr7g0223391 | 94.161 | 137 | 7 | 1 | 1 | 136 | 18 | 154 | 2.66e-90 | 258 |
| Msa1011850 | MtrunA17_Chr7g0223401 | 90.511 | 137 | 12 | 1 | 1 | 136 | 6 | 142 | 2.64e-86 | 248 |
| Msa1011850 | MtrunA17_Chr8g0356181 | 48.571 | 140 | 68 | 2 | 1 | 136 | 1 | 140 | 1.20e-40 | 132 |
| Msa1011850 | MtrunA17_Chr1g0190921 | 36.641 | 131 | 77 | 2 | 1 | 130 | 1 | 126 | 4.06e-19 | 77.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1011850 | AT2G44310.1 | 45.985 | 137 | 71 | 2 | 1 | 134 | 1 | 137 | 2.82e-37 | 124 |
Find 26 sgRNAs with CRISPR-Local
Find 27 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAAACACGAGCAAGCTCATT+TGG | 0.308059 | 7_1:+16659430 | None:intergenic |
| CATTTAAGCAGCCACTTTAC+CGG | 0.324149 | 7_1:+16659234 | None:intergenic |
| CTTGAATGGCACGGTTGATC+TGG | 0.357110 | 7_1:-16659377 | Msa1011850:CDS |
| TATTAAATGCTTCATCTTCA+AGG | 0.376744 | 7_1:+16659584 | None:intergenic |
| CATGCCAATTCAAATGGTCT+TGG | 0.391639 | 7_1:-16659296 | Msa1011850:CDS |
| TCTTCCAAGACCATTTGAAT+TGG | 0.395041 | 7_1:+16659292 | None:intergenic |
| TTAATAGCTCAGTTGGAAAT+CGG | 0.432475 | 7_1:-16659567 | Msa1011850:CDS |
| GAAGCATTTAATAGCTCAGT+TGG | 0.438228 | 7_1:-16659574 | Msa1011850:CDS |
| ATGCTTCATCTTCAAGGAAG+TGG | 0.440600 | 7_1:+16659590 | None:intergenic |
| CTCCTTCAACATCTCATCAT+AGG | 0.454389 | 7_1:+16659507 | None:intergenic |
| AAACACGAGCAAGCTCATTT+GGG | 0.456333 | 7_1:+16659431 | None:intergenic |
| GGGTTTCATGCCAATTCAAA+TGG | 0.458206 | 7_1:-16659302 | Msa1011850:CDS |
| CTCCTATGATGAGATGTTGA+AGG | 0.467641 | 7_1:-16659509 | Msa1011850:CDS |
| CAGTTCGATCACAACTTGAA+TGG | 0.477280 | 7_1:-16659391 | Msa1011850:CDS |
| GAAGGAGCTCAAGTGCTTGA+GGG | 0.496779 | 7_1:-16659491 | Msa1011850:CDS |
| GCTTGCAATGGCAGATGGAA+TGG | 0.514699 | 7_1:-16659323 | Msa1011850:CDS |
| ATAGCAGTTGAAAGAGAGTC+CGG | 0.521949 | 7_1:-16659253 | Msa1011850:CDS |
| CTTGCAATGGCAGATGGAAT+GGG | 0.535718 | 7_1:-16659322 | Msa1011850:CDS |
| TGAAAGAGAGTCCGGTAAAG+TGG | 0.542820 | 7_1:-16659245 | Msa1011850:CDS |
| TGAAGGAGCTCAAGTGCTTG+AGG | 0.550425 | 7_1:-16659492 | Msa1011850:CDS |
| ATGATGCTTGCAATGGCAGA+TGG | 0.563021 | 7_1:-16659328 | Msa1011850:CDS |
| GACCATCGTTATTTGTGTCG+AGG | 0.582987 | 7_1:+16659536 | None:intergenic |
| TTCAAGGAAGTGGACAATTG+TGG | 0.596143 | 7_1:+16659600 | None:intergenic |
| TAAGCAAATGATGCTTGCAA+TGG | 0.606338 | 7_1:-16659335 | Msa1011850:CDS |
| CGATCACAACTTGAATGGCA+CGG | 0.606932 | 7_1:-16659386 | Msa1011850:CDS |
| TGCCTCGACACAAATAACGA+TGG | 0.697379 | 7_1:-16659538 | Msa1011850:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATGAGTGTAGAAATTTTAGA+TGG | - | chr7_1:16659237-16659256 | Msa1011850:CDS | 25.0% |
| ! | TATTAAATGCTTCATCTTCA+AGG | + | chr7_1:16659281-16659300 | None:intergenic | 25.0% |
| TTAATAGCTCAGTTGGAAAT+CGG | - | chr7_1:16659295-16659314 | Msa1011850:CDS | 30.0% | |
| ! | GAAGCATTTAATAGCTCAGT+TGG | - | chr7_1:16659288-16659307 | Msa1011850:CDS | 35.0% |
| !!! | AGGGTTTTTGAAACACACTT+TGG | - | chr7_1:16659390-16659409 | Msa1011850:CDS | 35.0% |
| TAAGCAAATGATGCTTGCAA+TGG | - | chr7_1:16659527-16659546 | Msa1011850:CDS | 35.0% | |
| TCTTCCAAGACCATTTGAAT+TGG | + | chr7_1:16659573-16659592 | None:intergenic | 35.0% | |
| TTCAAGGAAGTGGACAATTG+TGG | + | chr7_1:16659265-16659284 | None:intergenic | 40.0% | |
| ATGCTTCATCTTCAAGGAAG+TGG | + | chr7_1:16659275-16659294 | None:intergenic | 40.0% | |
| CTCCTATGATGAGATGTTGA+AGG | - | chr7_1:16659353-16659372 | Msa1011850:CDS | 40.0% | |
| CTCCTTCAACATCTCATCAT+AGG | + | chr7_1:16659358-16659377 | None:intergenic | 40.0% | |
| AAACACGAGCAAGCTCATTT+GGG | + | chr7_1:16659434-16659453 | None:intergenic | 40.0% | |
| TAAACACGAGCAAGCTCATT+TGG | + | chr7_1:16659435-16659454 | None:intergenic | 40.0% | |
| CAGTTCGATCACAACTTGAA+TGG | - | chr7_1:16659471-16659490 | Msa1011850:CDS | 40.0% | |
| GGGTTTCATGCCAATTCAAA+TGG | - | chr7_1:16659560-16659579 | Msa1011850:CDS | 40.0% | |
| CATGCCAATTCAAATGGTCT+TGG | - | chr7_1:16659566-16659585 | Msa1011850:CDS | 40.0% | |
| ATAGCAGTTGAAAGAGAGTC+CGG | - | chr7_1:16659609-16659628 | Msa1011850:CDS | 40.0% | |
| TGCCTCGACACAAATAACGA+TGG | - | chr7_1:16659324-16659343 | Msa1011850:CDS | 45.0% | |
| GACCATCGTTATTTGTGTCG+AGG | + | chr7_1:16659329-16659348 | None:intergenic | 45.0% | |
| CGATCACAACTTGAATGGCA+CGG | - | chr7_1:16659476-16659495 | Msa1011850:CDS | 45.0% | |
| ATGATGCTTGCAATGGCAGA+TGG | - | chr7_1:16659534-16659553 | Msa1011850:CDS | 45.0% | |
| CTTGCAATGGCAGATGGAAT+GGG | - | chr7_1:16659540-16659559 | Msa1011850:CDS | 45.0% | |
| TGAAAGAGAGTCCGGTAAAG+TGG | - | chr7_1:16659617-16659636 | Msa1011850:CDS | 45.0% | |
| TGAAGGAGCTCAAGTGCTTG+AGG | - | chr7_1:16659370-16659389 | Msa1011850:CDS | 50.0% | |
| GAAGGAGCTCAAGTGCTTGA+GGG | - | chr7_1:16659371-16659390 | Msa1011850:CDS | 50.0% | |
| CTTGAATGGCACGGTTGATC+TGG | - | chr7_1:16659485-16659504 | Msa1011850:CDS | 50.0% | |
| GCTTGCAATGGCAGATGGAA+TGG | - | chr7_1:16659539-16659558 | Msa1011850:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_1 | gene | 16659237 | 16659647 | 16659237 | ID=Msa1011850;Name=Msa1011850 |
| chr7_1 | mRNA | 16659237 | 16659647 | 16659237 | ID=Msa1011850-mRNA-1;Parent=Msa1011850;Name=Msa1011850-mRNA-1;_AED=0.34;_eAED=0.34;_QI=0|-1|0|1|-1|1|1|0|136 |
| chr7_1 | exon | 16659237 | 16659647 | 16659237 | ID=Msa1011850-mRNA-1:exon:4237;Parent=Msa1011850-mRNA-1 |
| chr7_1 | CDS | 16659237 | 16659647 | 16659237 | ID=Msa1011850-mRNA-1:cds;Parent=Msa1011850-mRNA-1 |
| Gene Sequence |
| Protein sequence |
