Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1053300 | XP_013447886.1 | 94.161 | 137 | 7 | 1 | 1 | 136 | 1 | 137 | 1.68e-86 | 258 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1053300 | A0A072TYJ6 | 94.161 | 137 | 7 | 1 | 1 | 136 | 1 | 137 | 8.03e-87 | 258 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa1011800 | Msa1053300 | 0.801856 | 7.392268e-49 | -8.615850e-47 |
| Msa1011810 | Msa1053300 | 0.834390 | 3.081727e-56 | -8.615850e-47 |
| Msa1011830 | Msa1053300 | 0.833407 | 5.427858e-56 | -8.615850e-47 |
| Msa1011850 | Msa1053300 | 0.840298 | 9.460960e-58 | -8.615850e-47 |
| Msa1053300 | Msa1053320 | 0.868987 | 4.337362e-66 | -8.615850e-47 |
| Msa1053300 | Msa1053340 | 0.818674 | 1.732671e-52 | -8.615850e-47 |
| Msa1053300 | Msa1095410 | 0.807320 | 5.353130e-50 | -8.615850e-47 |
| Msa1053300 | Msa1095450 | 0.825500 | 4.531832e-54 | -8.615850e-47 |
| Msa1053300 | Msa1142590 | 0.824665 | 7.137606e-54 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1053300 | MtrunA17_Chr7g0223391 | 94.161 | 137 | 7 | 1 | 1 | 136 | 18 | 154 | 2.66e-90 | 258 |
| Msa1053300 | MtrunA17_Chr7g0223401 | 90.511 | 137 | 12 | 1 | 1 | 136 | 6 | 142 | 2.64e-86 | 248 |
| Msa1053300 | MtrunA17_Chr8g0356181 | 48.571 | 140 | 68 | 2 | 1 | 136 | 1 | 140 | 1.20e-40 | 132 |
| Msa1053300 | MtrunA17_Chr1g0190921 | 36.641 | 131 | 77 | 2 | 1 | 130 | 1 | 126 | 4.06e-19 | 77.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1053300 | AT2G44310.1 | 45.985 | 137 | 71 | 2 | 1 | 134 | 1 | 137 | 2.82e-37 | 124 |
Find 26 sgRNAs with CRISPR-Local
Find 28 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAAACACGAGCAAGCTCATT+TGG | 0.308059 | 7_2:+22713935 | None:intergenic |
| CATTTAAGCAGCCACTTTAC+CGG | 0.324149 | 7_2:+22713739 | None:intergenic |
| CTTGAATGGCACGGTTGATC+TGG | 0.357110 | 7_2:-22713882 | Msa1053300:CDS |
| TATTAAATGCTTCATCTTCA+AGG | 0.376744 | 7_2:+22714089 | None:intergenic |
| CATGCCAATTCAAATGGTCT+TGG | 0.391639 | 7_2:-22713801 | Msa1053300:CDS |
| TCTTCCAAGACCATTTGAAT+TGG | 0.395041 | 7_2:+22713797 | None:intergenic |
| TTAATAGCTCAGTTGGAAAT+CGG | 0.432475 | 7_2:-22714072 | Msa1053300:CDS |
| GAAGCATTTAATAGCTCAGT+TGG | 0.438228 | 7_2:-22714079 | Msa1053300:CDS |
| ATGCTTCATCTTCAAGGAAG+TGG | 0.440600 | 7_2:+22714095 | None:intergenic |
| CTCCTTCAACATCTCATCAT+AGG | 0.454389 | 7_2:+22714012 | None:intergenic |
| AAACACGAGCAAGCTCATTT+GGG | 0.456333 | 7_2:+22713936 | None:intergenic |
| GGGTTTCATGCCAATTCAAA+TGG | 0.458206 | 7_2:-22713807 | Msa1053300:CDS |
| CTCCTATGATGAGATGTTGA+AGG | 0.467641 | 7_2:-22714014 | Msa1053300:CDS |
| CAGTTCGATCACAACTTGAA+TGG | 0.477280 | 7_2:-22713896 | Msa1053300:CDS |
| GAAGGAGCTCAAGTGCTTGA+GGG | 0.496779 | 7_2:-22713996 | Msa1053300:CDS |
| GCTTGCAATGGCAGATGGAA+TGG | 0.514699 | 7_2:-22713828 | Msa1053300:CDS |
| CTTGCAATGGCAGATGGAAT+GGG | 0.535718 | 7_2:-22713827 | Msa1053300:CDS |
| TGAAAGAGAGTCCGGTAAAG+TGG | 0.542820 | 7_2:-22713750 | Msa1053300:CDS |
| ATAGCCGTTGAAAGAGAGTC+CGG | 0.546530 | 7_2:-22713758 | Msa1053300:CDS |
| TGAAGGAGCTCAAGTGCTTG+AGG | 0.550425 | 7_2:-22713997 | Msa1053300:CDS |
| TTTACCGGACTCTCTTTCAA+CGG | 0.556793 | 7_2:+22713754 | None:intergenic |
| ATGATGCTTGCAATGGCAGA+TGG | 0.563021 | 7_2:-22713833 | Msa1053300:CDS |
| GACCATCGTTATTTGTGTCG+AGG | 0.582987 | 7_2:+22714041 | None:intergenic |
| TAAGCAAATGATGCTTGCAA+TGG | 0.606338 | 7_2:-22713840 | Msa1053300:CDS |
| CGATCACAACTTGAATGGCA+CGG | 0.606932 | 7_2:-22713891 | Msa1053300:CDS |
| TGCCTCGACACAAATAACGA+TGG | 0.697379 | 7_2:-22714043 | Msa1053300:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATGAGTGTAGAAATTTTAGA+TGG | - | chr7_2:22713742-22713761 | Msa1053300:CDS | 25.0% |
| ! | TATTAAATGCTTCATCTTCA+AGG | + | chr7_2:22713786-22713805 | None:intergenic | 25.0% |
| TTAATAGCTCAGTTGGAAAT+CGG | - | chr7_2:22713800-22713819 | Msa1053300:CDS | 30.0% | |
| ! | GAAGCATTTAATAGCTCAGT+TGG | - | chr7_2:22713793-22713812 | Msa1053300:CDS | 35.0% |
| !!! | AGGGTTTTTGAAACACACTT+TGG | - | chr7_2:22713895-22713914 | Msa1053300:CDS | 35.0% |
| TAAGCAAATGATGCTTGCAA+TGG | - | chr7_2:22714032-22714051 | Msa1053300:CDS | 35.0% | |
| TCTTCCAAGACCATTTGAAT+TGG | + | chr7_2:22714078-22714097 | None:intergenic | 35.0% | |
| ATGCTTCATCTTCAAGGAAG+TGG | + | chr7_2:22713780-22713799 | None:intergenic | 40.0% | |
| CTCCTATGATGAGATGTTGA+AGG | - | chr7_2:22713858-22713877 | Msa1053300:CDS | 40.0% | |
| CTCCTTCAACATCTCATCAT+AGG | + | chr7_2:22713863-22713882 | None:intergenic | 40.0% | |
| AAACACGAGCAAGCTCATTT+GGG | + | chr7_2:22713939-22713958 | None:intergenic | 40.0% | |
| TAAACACGAGCAAGCTCATT+TGG | + | chr7_2:22713940-22713959 | None:intergenic | 40.0% | |
| CAGTTCGATCACAACTTGAA+TGG | - | chr7_2:22713976-22713995 | Msa1053300:CDS | 40.0% | |
| GGGTTTCATGCCAATTCAAA+TGG | - | chr7_2:22714065-22714084 | Msa1053300:CDS | 40.0% | |
| CATGCCAATTCAAATGGTCT+TGG | - | chr7_2:22714071-22714090 | Msa1053300:CDS | 40.0% | |
| !! | CTCTTTCAACGGCTATTTTG+AGG | + | chr7_2:22714110-22714129 | None:intergenic | 40.0% |
| TTTACCGGACTCTCTTTCAA+CGG | + | chr7_2:22714121-22714140 | None:intergenic | 40.0% | |
| TGCCTCGACACAAATAACGA+TGG | - | chr7_2:22713829-22713848 | Msa1053300:CDS | 45.0% | |
| GACCATCGTTATTTGTGTCG+AGG | + | chr7_2:22713834-22713853 | None:intergenic | 45.0% | |
| CGATCACAACTTGAATGGCA+CGG | - | chr7_2:22713981-22714000 | Msa1053300:CDS | 45.0% | |
| ATGATGCTTGCAATGGCAGA+TGG | - | chr7_2:22714039-22714058 | Msa1053300:CDS | 45.0% | |
| CTTGCAATGGCAGATGGAAT+GGG | - | chr7_2:22714045-22714064 | Msa1053300:CDS | 45.0% | |
| ATAGCCGTTGAAAGAGAGTC+CGG | - | chr7_2:22714114-22714133 | Msa1053300:CDS | 45.0% | |
| TGAAAGAGAGTCCGGTAAAG+TGG | - | chr7_2:22714122-22714141 | Msa1053300:CDS | 45.0% | |
| TGAAGGAGCTCAAGTGCTTG+AGG | - | chr7_2:22713875-22713894 | Msa1053300:CDS | 50.0% | |
| GAAGGAGCTCAAGTGCTTGA+GGG | - | chr7_2:22713876-22713895 | Msa1053300:CDS | 50.0% | |
| CTTGAATGGCACGGTTGATC+TGG | - | chr7_2:22713990-22714009 | Msa1053300:CDS | 50.0% | |
| GCTTGCAATGGCAGATGGAA+TGG | - | chr7_2:22714044-22714063 | Msa1053300:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_2 | gene | 22713742 | 22714152 | 22713742 | ID=Msa1053300;Name=Msa1053300 |
| chr7_2 | mRNA | 22713742 | 22714152 | 22713742 | ID=Msa1053300-mRNA-1;Parent=Msa1053300;Name=Msa1053300-mRNA-1;_AED=0.25;_eAED=0.25;_QI=0|-1|0|1|-1|1|1|0|136 |
| chr7_2 | exon | 22713742 | 22714152 | 22713742 | ID=Msa1053300-mRNA-1:exon:5153;Parent=Msa1053300-mRNA-1 |
| chr7_2 | CDS | 22713742 | 22714152 | 22713742 | ID=Msa1053300-mRNA-1:cds;Parent=Msa1053300-mRNA-1 |
| Gene Sequence |
| Protein sequence |
