AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1102430

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1102430 MtrunA17_Chr7g0231911 97.479 119 3 0 4 122 196 314 4.51e-82 243
Msa1102430 MtrunA17_Chr4g0046991 79.412 34 7 0 4 37 120 153 2.12e-11 57.4
Msa1102430 MtrunA17_Chr7g0270541 65.789 38 13 0 4 41 192 229 5.86e-11 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1102430 AT3G15270.1 59.184 49 20 0 4 52 110 158 4.82e-12 60.1
Msa1102430 AT3G60030.1 65.789 38 13 0 4 41 174 211 1.24e-11 60.8
Msa1102430 AT2G33810.1 80.645 31 6 0 4 34 101 131 2.86e-11 57.0

Find 51 sgRNAs with CRISPR-Local

Find 236 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGTTTATTTCTCTAAACTTT+AGG 0.253414 7_3:+35287547 None:intergenic
AAACTTTAGGCCGTGGTTCT+TGG 0.255544 7_3:+35287560 None:intergenic
AGCCAGTAAGTCCGAATAAT+TGG 0.259835 7_3:+35287623 None:intergenic
ATCCAATTATTCGGACTTAC+TGG 0.273786 7_3:-35287625 Msa1102430:three_prime_UTR
CATATGTTGTGGGAAATAAT+TGG 0.328845 7_3:+35287830 None:intergenic
TGTCATCTCGGACTGATTCA+TGG 0.336380 7_3:-35288010 Msa1102430:CDS
ACATTAGTGATGGTTTCTTA+TGG 0.352970 7_3:-35287796 Msa1102430:exon
CTGATCATCATCCAATTATT+CGG 0.390385 7_3:-35287634 Msa1102430:three_prime_UTR
AGAAAACCGAGCATCGATAA+TGG 0.432077 7_3:-35287931 Msa1102430:CDS
CTTTCTCTCTTCTGTCATCT+CGG 0.450629 7_3:-35288022 Msa1102430:CDS
GTTTCTTATGGCACGGAGCT+TGG 0.450748 7_3:-35287784 Msa1102430:three_prime_UTR
AGTTGTAGAAGGAGACTGGC+AGG 0.470576 7_3:-35289067 Msa1102430:CDS
GCCTAGCCATTATCGATGCT+CGG 0.471586 7_3:+35287925 None:intergenic
GCTAGCATCCTGGTTCTAGA+AGG 0.484857 7_3:+35287446 None:intergenic
AATTCCTCCCAACAGAATCA+TGG 0.485519 7_3:+35289017 None:intergenic
GAACCAGGATGCTAGCTAGC+TGG 0.489896 7_3:-35287439 Msa1102430:three_prime_UTR
TATGGCACGGAGCTTGGATA+CGG 0.492925 7_3:-35287778 Msa1102430:three_prime_UTR
AAGAAACAACTCAATATATG+AGG 0.496341 7_3:+35287691 None:intergenic
TTTCTCTAAACTTTAGGCCG+TGG 0.498084 7_3:+35287553 None:intergenic
AAGAAGTTGTAGAAGGAGAC+TGG 0.506657 7_3:-35289071 Msa1102430:CDS
AGTCAGTCGATCAAAATTGT+TGG 0.507021 7_3:-35287728 Msa1102430:three_prime_UTR
CCATGCCGTGGAAGATTACA+AGG 0.507477 7_3:-35287868 Msa1102430:CDS
GACTGATTCATGGCTTTCAC+CGG 0.514906 7_3:-35288000 Msa1102430:CDS
CTAGCTAGCTGGACTAGTTA+CGG 0.516257 7_3:-35287428 Msa1102430:three_prime_UTR
GGAAATAATTGGACTCGGAA+TGG 0.522818 7_3:+35287841 None:intergenic
AGAGCTCCCATGATTCTGTT+GGG 0.525869 7_3:-35289024 Msa1102430:CDS
AAGAGCTCCCATGATTCTGT+TGG 0.535482 7_3:-35289025 Msa1102430:CDS
CATCAAATTCTGACACAACA+TGG 0.543897 7_3:+35289101 None:intergenic
TTATTCGGACTTACTGGCTG+TGG 0.546694 7_3:-35287619 Msa1102430:three_prime_UTR
TGCAACAATCTTACCTTGTG+AGG 0.547389 7_3:+35288994 None:intergenic
TCGACTGACTGCGATGGCTA+TGG 0.553452 7_3:+35287741 None:intergenic
ATTCCTCCCAACAGAATCAT+GGG 0.563033 7_3:+35289018 None:intergenic
GATCTCCTTGTAATCTTCCA+CGG 0.568098 7_3:+35287863 None:intergenic
AATACGTCTTAGACAGAGAC+TGG 0.570424 7_3:-35287902 Msa1102430:CDS
TTGTGGGAAATAATTGGACT+CGG 0.572180 7_3:+35287836 None:intergenic
CCTTGTAATCTTCCACGGCA+TGG 0.577549 7_3:+35287868 None:intergenic
TTCCGCAGACACATTAGTGA+TGG 0.581420 7_3:-35287806 Msa1102430:exon
AGTGATGGTTTCTTATGGCA+CGG 0.585253 7_3:-35287791 Msa1102430:three_prime_UTR
AGTCCAGCTAGCTAGCATCC+TGG 0.586047 7_3:+35287436 None:intergenic
CATCTTACAGAGAGGTCAGC+CGG 0.592957 7_3:+35287981 None:intergenic
TCTTGGATCAAGATTTCATG+CGG 0.608746 7_3:+35287577 None:intergenic
TCTGCGGAAACATATGTTGT+GGG 0.616543 7_3:+35287820 None:intergenic
ACCGAGCATCGATAATGGCT+AGG 0.618479 7_3:-35287926 Msa1102430:CDS
GCATTTGCAGCACCATGCCG+TGG 0.620143 7_3:-35287880 Msa1102430:CDS
AACCATCACTAATGTGTCTG+CGG 0.630280 7_3:+35287804 None:intergenic
GTTGTAGAAGGAGACTGGCA+GGG 0.647920 7_3:-35289066 Msa1102430:CDS
GTTGGGAGGAATTCCTCACA+AGG 0.661568 7_3:-35289007 Msa1102430:intron
GTCTGCGGAAACATATGTTG+TGG 0.669116 7_3:+35287819 None:intergenic
GCTCCCATGATTCTGTTGGG+AGG 0.673767 7_3:-35289021 Msa1102430:CDS
AAATCTTGATCCAAGAACCA+CGG 0.687026 7_3:-35287570 Msa1102430:three_prime_UTR
CAGCACTGCATCTTACAGAG+AGG 0.726579 7_3:+35287973 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GTTTTTAATTTTATGTTTTA+GGG - chr7_3:35287378-35287397 Msa1102430:three_prime_UTR 10.0%
!!! AACCTTTTTTTTAATTTTTT+TGG - chr7_3:35288234-35288253 Msa1102430:intron 10.0%
!! AACCAAAAAAATTAAAAAAA+AGG + chr7_3:35288239-35288258 None:intergenic 10.0%
!!! GTTTTTAATTTTATGTTTTA+GGG - chr7_3:35287378-35287397 Msa1102430:three_prime_UTR 10.0%
!!! AACCTTTTTTTTAATTTTTT+TGG - chr7_3:35288234-35288253 Msa1102430:intron 10.0%
!! AACCAAAAAAATTAAAAAAA+AGG + chr7_3:35288239-35288258 None:intergenic 10.0%
!!! GGTTTTTAATTTTATGTTTT+AGG - chr7_3:35287377-35287396 Msa1102430:three_prime_UTR 15.0%
!! GATAATTAATATGAAAAATG+TGG - chr7_3:35287422-35287441 Msa1102430:three_prime_UTR 15.0%
!! TTCAAAAAAAGTAAATATCA+TGG + chr7_3:35287644-35287663 None:intergenic 15.0%
!!! TTATATATTTTCAATGCATT+AGG + chr7_3:35287755-35287774 None:intergenic 15.0%
!! AAAAAAAATATTGAAAACGT+AGG + chr7_3:35287991-35288010 None:intergenic 15.0%
!!! CGTTTTCAATATTTTTTTTA+AGG - chr7_3:35287992-35288011 Msa1102430:CDS 15.0%
!! GAAAACTAAAATAAACATAA+GGG + chr7_3:35288359-35288378 None:intergenic 15.0%
!! TGAAAACTAAAATAAACATA+AGG + chr7_3:35288358-35288377 None:intergenic 15.0%
!!! TTGAAACTTTTAAAACATAA+GGG + chr7_3:35288067-35288086 None:intergenic 15.0%
!! TGAAAACTAAAATAAACATA+AGG - chr7_3:35288358-35288377 Msa1102430:intron 15.0%
!! GAAAACTAAAATAAACATAA+GGG - chr7_3:35288359-35288378 Msa1102430:intron 15.0%
!!! TATTTTTAGGGATATTAAAA+TGG + chr7_3:35289083-35289102 None:intergenic 15.0%
!!! GTTCTTTACTTTTATTTTTA+GGG + chr7_3:35289095-35289114 None:intergenic 15.0%
!!! AGTTCTTTACTTTTATTTTT+AGG + chr7_3:35289096-35289115 None:intergenic 15.0%
!!! GGTTTTTAATTTTATGTTTT+AGG - chr7_3:35287377-35287396 Msa1102430:three_prime_UTR 15.0%
!! GATAATTAATATGAAAAATG+TGG - chr7_3:35287422-35287441 Msa1102430:three_prime_UTR 15.0%
!! TTCAAAAAAAGTAAATATCA+TGG + chr7_3:35287644-35287663 None:intergenic 15.0%
!!! TTATATATTTTCAATGCATT+AGG + chr7_3:35287755-35287774 None:intergenic 15.0%
!! AAAAAAAATATTGAAAACGT+AGG + chr7_3:35287991-35288010 None:intergenic 15.0%
!!! CGTTTTCAATATTTTTTTTA+AGG - chr7_3:35287992-35288011 Msa1102430:CDS 15.0%
!! GAAAACTAAAATAAACATAA+GGG + chr7_3:35288359-35288378 None:intergenic 15.0%
!! TGAAAACTAAAATAAACATA+AGG + chr7_3:35288358-35288377 None:intergenic 15.0%
!!! TTGAAACTTTTAAAACATAA+GGG + chr7_3:35288067-35288086 None:intergenic 15.0%
!! TGAAAACTAAAATAAACATA+AGG - chr7_3:35288358-35288377 Msa1102430:intron 15.0%
!! GAAAACTAAAATAAACATAA+GGG - chr7_3:35288359-35288378 Msa1102430:intron 15.0%
!!! TATTTTTAGGGATATTAAAA+TGG + chr7_3:35289083-35289102 None:intergenic 15.0%
!!! GTTCTTTACTTTTATTTTTA+GGG + chr7_3:35289095-35289114 None:intergenic 15.0%
!!! AGTTCTTTACTTTTATTTTT+AGG + chr7_3:35289096-35289115 None:intergenic 15.0%
!! AATTAATATGAAAAATGTGG+TGG - chr7_3:35287425-35287444 Msa1102430:three_prime_UTR 20.0%
!!! ATAAGAGTACACAATTTTTT+TGG - chr7_3:35287771-35287790 Msa1102430:three_prime_UTR 20.0%
!!! GTTTATTTTAGTTTTCATGT+TGG - chr7_3:35288040-35288059 Msa1102430:intron 20.0%
!!! CTTGAAACTTTTAAAACATA+AGG + chr7_3:35288068-35288087 None:intergenic 20.0%
!!! ATGTTTTAAAAGTTTCAAGT+TGG - chr7_3:35288069-35288088 Msa1102430:intron 20.0%
!!! TTGAAACTTTTAAAACGTAA+TGG + chr7_3:35288328-35288347 None:intergenic 20.0%
!! AATCTTTAAACGTTAAATGA+TGG + chr7_3:35288162-35288181 None:intergenic 20.0%
!! TCATTTAACGTTTAAAGATT+TGG - chr7_3:35288162-35288181 Msa1102430:intron 20.0%
!! CATTTAACGTTTAAAGATTT+GGG - chr7_3:35288163-35288182 Msa1102430:intron 20.0%
!!! GTTTATTTTAGTTTTCAAGT+TGG + chr7_3:35288355-35288374 None:intergenic 20.0%
!! TACTAAGCAGATAAAAAAAA+GGG + chr7_3:35288402-35288421 None:intergenic 20.0%
!! TTACTAAGCAGATAAAAAAA+AGG + chr7_3:35288403-35288422 None:intergenic 20.0%
!!! TCAATAAAATTTTCAAGCAA+TGG + chr7_3:35289240-35289259 None:intergenic 20.0%
!! AATTAATATGAAAAATGTGG+TGG - chr7_3:35287425-35287444 Msa1102430:three_prime_UTR 20.0%
!!! ATAAGAGTACACAATTTTTT+TGG - chr7_3:35287771-35287790 Msa1102430:three_prime_UTR 20.0%
!!! GTTTATTTTAGTTTTCATGT+TGG - chr7_3:35288040-35288059 Msa1102430:intron 20.0%
!!! CTTGAAACTTTTAAAACATA+AGG + chr7_3:35288068-35288087 None:intergenic 20.0%
!!! ATGTTTTAAAAGTTTCAAGT+TGG - chr7_3:35288069-35288088 Msa1102430:intron 20.0%
!!! TTGAAACTTTTAAAACGTAA+TGG + chr7_3:35288328-35288347 None:intergenic 20.0%
!! AATCTTTAAACGTTAAATGA+TGG + chr7_3:35288162-35288181 None:intergenic 20.0%
!! TCATTTAACGTTTAAAGATT+TGG - chr7_3:35288162-35288181 Msa1102430:intron 20.0%
!! CATTTAACGTTTAAAGATTT+GGG - chr7_3:35288163-35288182 Msa1102430:intron 20.0%
!!! GTTTATTTTAGTTTTCAAGT+TGG + chr7_3:35288355-35288374 None:intergenic 20.0%
!! TACTAAGCAGATAAAAAAAA+GGG + chr7_3:35288402-35288421 None:intergenic 20.0%
!! TTACTAAGCAGATAAAAAAA+AGG + chr7_3:35288403-35288422 None:intergenic 20.0%
!!! TCAATAAAATTTTCAAGCAA+TGG + chr7_3:35289240-35289259 None:intergenic 20.0%
!! TCAAAGGTGTAAAAATTATG+TGG - chr7_3:35287303-35287322 Msa1102430:three_prime_UTR 25.0%
!! CAAAGGTGTAAAAATTATGT+GGG - chr7_3:35287304-35287323 Msa1102430:three_prime_UTR 25.0%
! ATTCAAAAAGAAGTTGTAGA+AGG - chr7_3:35287492-35287511 Msa1102430:three_prime_UTR 25.0%
! TACAATTGCATTCAATATCA+TGG + chr7_3:35287677-35287696 None:intergenic 25.0%
!! GAAGATACTTTAAAGATAGA+TGG - chr7_3:35287903-35287922 Msa1102430:CDS 25.0%
!!! ACGTTTTAAAAGTTTCAAGT+TGG - chr7_3:35288098-35288117 Msa1102430:intron 25.0%
!! TTAAACGTTAAATGATGGAA+AGG + chr7_3:35288157-35288176 None:intergenic 25.0%
!!! ACGTTTTAAAAGTTTCAAGT+TGG + chr7_3:35288098-35288117 None:intergenic 25.0%
!!! GTTTTTTGTTTCTTGTAATC+AGG - chr7_3:35288517-35288536 Msa1102430:intron 25.0%
! AAGAAACAACTCAATATATG+AGG + chr7_3:35288882-35288901 None:intergenic 25.0%
!! CGTTTATTTCTCTAAACTTT+AGG + chr7_3:35289026-35289045 None:intergenic 25.0%
!! TCATAAAACAGTGTACTTTT+AGG + chr7_3:35289051-35289070 None:intergenic 25.0%
!! TCAAAGGTGTAAAAATTATG+TGG - chr7_3:35287303-35287322 Msa1102430:three_prime_UTR 25.0%
!! CAAAGGTGTAAAAATTATGT+GGG - chr7_3:35287304-35287323 Msa1102430:three_prime_UTR 25.0%
! ATTCAAAAAGAAGTTGTAGA+AGG - chr7_3:35287492-35287511 Msa1102430:three_prime_UTR 25.0%
! TACAATTGCATTCAATATCA+TGG + chr7_3:35287677-35287696 None:intergenic 25.0%
!! GAAGATACTTTAAAGATAGA+TGG - chr7_3:35287903-35287922 Msa1102430:CDS 25.0%
!!! ACGTTTTAAAAGTTTCAAGT+TGG - chr7_3:35288098-35288117 Msa1102430:intron 25.0%
!! TTAAACGTTAAATGATGGAA+AGG + chr7_3:35288157-35288176 None:intergenic 25.0%
!!! ACGTTTTAAAAGTTTCAAGT+TGG + chr7_3:35288098-35288117 None:intergenic 25.0%
!!! GTTTTTTGTTTCTTGTAATC+AGG - chr7_3:35288517-35288536 Msa1102430:intron 25.0%
! AAGAAACAACTCAATATATG+AGG + chr7_3:35288882-35288901 None:intergenic 25.0%
!! CGTTTATTTCTCTAAACTTT+AGG + chr7_3:35289026-35289045 None:intergenic 25.0%
!! TCATAAAACAGTGTACTTTT+AGG + chr7_3:35289051-35289070 None:intergenic 25.0%
TATTTGCACACAATGTTGTA+TGG - chr7_3:35287356-35287375 Msa1102430:three_prime_UTR 30.0%
!!! TGTTTTAGGGATTTGTTCAT+TGG - chr7_3:35287391-35287410 Msa1102430:three_prime_UTR 30.0%
TAATATGAAAAATGTGGTGG+TGG - chr7_3:35287428-35287447 Msa1102430:three_prime_UTR 30.0%
AATATGAAAAATGTGGTGGT+GGG - chr7_3:35287429-35287448 Msa1102430:three_prime_UTR 30.0%
AAGGACTATAGTTACTCTAT+CGG + chr7_3:35287810-35287829 None:intergenic 30.0%
! ATGTAAATTTGAGACAGTCA+AGG + chr7_3:35287829-35287848 None:intergenic 30.0%
!!! GTATCTTCTCGTTGTTATTT+TGG + chr7_3:35287891-35287910 None:intergenic 30.0%
CATAGTAGACAATATGTTGA+TGG - chr7_3:35288488-35288507 Msa1102430:intron 30.0%
!! TTTTGTTTCTTGTAATCAGG+TGG - chr7_3:35288520-35288539 Msa1102430:intron 30.0%
! TTTGTTTCTTGTAATCAGGT+GGG - chr7_3:35288521-35288540 Msa1102430:intron 30.0%
CATATGTTGTGGGAAATAAT+TGG + chr7_3:35288743-35288762 None:intergenic 30.0%
ACATTAGTGATGGTTTCTTA+TGG - chr7_3:35288774-35288793 Msa1102430:intron 30.0%
CTGATCATCATCCAATTATT+CGG - chr7_3:35288936-35288955 Msa1102430:intron 30.0%
!!! TTTTTTTTCCTTCTAGAACC+AGG - chr7_3:35289116-35289135 Msa1102430:intron 30.0%
TATTTGCACACAATGTTGTA+TGG - chr7_3:35287356-35287375 Msa1102430:three_prime_UTR 30.0%
!!! TGTTTTAGGGATTTGTTCAT+TGG - chr7_3:35287391-35287410 Msa1102430:three_prime_UTR 30.0%
TAATATGAAAAATGTGGTGG+TGG - chr7_3:35287428-35287447 Msa1102430:three_prime_UTR 30.0%
AATATGAAAAATGTGGTGGT+GGG - chr7_3:35287429-35287448 Msa1102430:three_prime_UTR 30.0%
AAGGACTATAGTTACTCTAT+CGG + chr7_3:35287810-35287829 None:intergenic 30.0%
! ATGTAAATTTGAGACAGTCA+AGG + chr7_3:35287829-35287848 None:intergenic 30.0%
!!! GTATCTTCTCGTTGTTATTT+TGG + chr7_3:35287891-35287910 None:intergenic 30.0%
CATAGTAGACAATATGTTGA+TGG - chr7_3:35288488-35288507 Msa1102430:intron 30.0%
!! TTTTGTTTCTTGTAATCAGG+TGG - chr7_3:35288520-35288539 Msa1102430:intron 30.0%
! TTTGTTTCTTGTAATCAGGT+GGG - chr7_3:35288521-35288540 Msa1102430:intron 30.0%
CATATGTTGTGGGAAATAAT+TGG + chr7_3:35288743-35288762 None:intergenic 30.0%
ACATTAGTGATGGTTTCTTA+TGG - chr7_3:35288774-35288793 Msa1102430:intron 30.0%
CTGATCATCATCCAATTATT+CGG - chr7_3:35288936-35288955 Msa1102430:intron 30.0%
!!! TTTTTTTTCCTTCTAGAACC+AGG - chr7_3:35289116-35289135 Msa1102430:intron 30.0%
! AATTTTTACACCTTTGACCC+TGG + chr7_3:35287300-35287319 None:intergenic 35.0%
! ATATGAAAAATGTGGTGGTG+GGG - chr7_3:35287430-35287449 Msa1102430:three_prime_UTR 35.0%
CATCAAATTCTGACACAACA+TGG + chr7_3:35287472-35287491 None:intergenic 35.0%
ATCTTCCGTTCGTAATTTGA+TGG - chr7_3:35287864-35287883 Msa1102430:CDS 35.0%
TTGGTCCATCAAATTACGAA+CGG + chr7_3:35287872-35287891 None:intergenic 35.0%
! TTCCAAAGTGAGTAACGTTT+TGG - chr7_3:35288299-35288318 Msa1102430:intron 35.0%
!! GTAGACAATATGTTGATGGT+TGG - chr7_3:35288492-35288511 Msa1102430:intron 35.0%
TTGTGGGAAATAATTGGACT+CGG + chr7_3:35288737-35288756 None:intergenic 35.0%
AGTCAGTCGATCAAAATTGT+TGG - chr7_3:35288842-35288861 Msa1102430:intron 35.0%
ATCCAATTATTCGGACTTAC+TGG - chr7_3:35288945-35288964 Msa1102430:intron 35.0%
TCTTGGATCAAGATTTCATG+CGG + chr7_3:35288996-35289015 None:intergenic 35.0%
AAATCTTGATCCAAGAACCA+CGG - chr7_3:35289000-35289019 Msa1102430:intron 35.0%
! ATGTTTTGCATGACAACTCT+CGG - chr7_3:35289276-35289295 Msa1102430:intron 35.0%
! AATTTTTACACCTTTGACCC+TGG + chr7_3:35287300-35287319 None:intergenic 35.0%
! ATATGAAAAATGTGGTGGTG+GGG - chr7_3:35287430-35287449 Msa1102430:three_prime_UTR 35.0%
CATCAAATTCTGACACAACA+TGG + chr7_3:35287472-35287491 None:intergenic 35.0%
ATCTTCCGTTCGTAATTTGA+TGG - chr7_3:35287864-35287883 Msa1102430:CDS 35.0%
TTGGTCCATCAAATTACGAA+CGG + chr7_3:35287872-35287891 None:intergenic 35.0%
! TTCCAAAGTGAGTAACGTTT+TGG - chr7_3:35288299-35288318 Msa1102430:intron 35.0%
!! GTAGACAATATGTTGATGGT+TGG - chr7_3:35288492-35288511 Msa1102430:intron 35.0%
TTGTGGGAAATAATTGGACT+CGG + chr7_3:35288737-35288756 None:intergenic 35.0%
AGTCAGTCGATCAAAATTGT+TGG - chr7_3:35288842-35288861 Msa1102430:intron 35.0%
ATCCAATTATTCGGACTTAC+TGG - chr7_3:35288945-35288964 Msa1102430:intron 35.0%
TCTTGGATCAAGATTTCATG+CGG + chr7_3:35288996-35289015 None:intergenic 35.0%
AAATCTTGATCCAAGAACCA+CGG - chr7_3:35289000-35289019 Msa1102430:intron 35.0%
AAGAAGTTGTAGAAGGAGAC+TGG - chr7_3:35287499-35287518 Msa1102430:three_prime_UTR 40.0%
ATTCCTCCCAACAGAATCAT+GGG + chr7_3:35287555-35287574 None:intergenic 40.0%
AATTCCTCCCAACAGAATCA+TGG + chr7_3:35287556-35287575 None:intergenic 40.0%
TGCAACAATCTTACCTTGTG+AGG + chr7_3:35287579-35287598 None:intergenic 40.0%
GGACTAACTTATTCCAACCT+GGG + chr7_3:35288136-35288155 None:intergenic 40.0%
AGGACTAACTTATTCCAACC+TGG + chr7_3:35288137-35288156 None:intergenic 40.0%
GTCCAAAACGTTACTCACTT+TGG + chr7_3:35288304-35288323 None:intergenic 40.0%
!! GACAATATGTTGATGGTTGG+TGG - chr7_3:35288495-35288514 Msa1102430:intron 40.0%
CTTTCTCTCTTCTGTCATCT+CGG - chr7_3:35288548-35288567 Msa1102430:intron 40.0%
!! AGAAAACCGAGCATCGATAA+TGG - chr7_3:35288639-35288658 Msa1102430:intron 40.0%
AATACGTCTTAGACAGAGAC+TGG - chr7_3:35288668-35288687 Msa1102430:intron 40.0%
GATCTCCTTGTAATCTTCCA+CGG + chr7_3:35288710-35288729 None:intergenic 40.0%
GGAAATAATTGGACTCGGAA+TGG + chr7_3:35288732-35288751 None:intergenic 40.0%
TCTGCGGAAACATATGTTGT+GGG + chr7_3:35288753-35288772 None:intergenic 40.0%
AACCATCACTAATGTGTCTG+CGG + chr7_3:35288769-35288788 None:intergenic 40.0%
AGTGATGGTTTCTTATGGCA+CGG - chr7_3:35288779-35288798 Msa1102430:intron 40.0%
AGCCAGTAAGTCCGAATAAT+TGG + chr7_3:35288950-35288969 None:intergenic 40.0%
TTTCTCTAAACTTTAGGCCG+TGG + chr7_3:35289020-35289039 None:intergenic 40.0%
AAGAAGTTGTAGAAGGAGAC+TGG - chr7_3:35287499-35287518 Msa1102430:three_prime_UTR 40.0%
ATTCCTCCCAACAGAATCAT+GGG + chr7_3:35287555-35287574 None:intergenic 40.0%
AATTCCTCCCAACAGAATCA+TGG + chr7_3:35287556-35287575 None:intergenic 40.0%
TGCAACAATCTTACCTTGTG+AGG + chr7_3:35287579-35287598 None:intergenic 40.0%
GGACTAACTTATTCCAACCT+GGG + chr7_3:35288136-35288155 None:intergenic 40.0%
AGGACTAACTTATTCCAACC+TGG + chr7_3:35288137-35288156 None:intergenic 40.0%
GTCCAAAACGTTACTCACTT+TGG + chr7_3:35288304-35288323 None:intergenic 40.0%
!! GACAATATGTTGATGGTTGG+TGG - chr7_3:35288495-35288514 Msa1102430:intron 40.0%
CTTTCTCTCTTCTGTCATCT+CGG - chr7_3:35288548-35288567 Msa1102430:intron 40.0%
!! AGAAAACCGAGCATCGATAA+TGG - chr7_3:35288639-35288658 Msa1102430:intron 40.0%
AATACGTCTTAGACAGAGAC+TGG - chr7_3:35288668-35288687 Msa1102430:intron 40.0%
GATCTCCTTGTAATCTTCCA+CGG + chr7_3:35288710-35288729 None:intergenic 40.0%
GGAAATAATTGGACTCGGAA+TGG + chr7_3:35288732-35288751 None:intergenic 40.0%
TCTGCGGAAACATATGTTGT+GGG + chr7_3:35288753-35288772 None:intergenic 40.0%
AACCATCACTAATGTGTCTG+CGG + chr7_3:35288769-35288788 None:intergenic 40.0%
AGTGATGGTTTCTTATGGCA+CGG - chr7_3:35288779-35288798 Msa1102430:intron 40.0%
AGCCAGTAAGTCCGAATAAT+TGG + chr7_3:35288950-35288969 None:intergenic 40.0%
TTTCTCTAAACTTTAGGCCG+TGG + chr7_3:35289020-35289039 None:intergenic 40.0%
AAGAGCTCCCATGATTCTGT+TGG - chr7_3:35287545-35287564 Msa1102430:three_prime_UTR 45.0%
AGAGCTCCCATGATTCTGTT+GGG - chr7_3:35287546-35287565 Msa1102430:three_prime_UTR 45.0%
! GTTGGTTCAAGATGTTACCC+AGG - chr7_3:35288116-35288135 Msa1102430:intron 45.0%
GTTCAAGATGTTACCCAGGT+TGG - chr7_3:35288120-35288139 Msa1102430:intron 45.0%
! TGTCATCTCGGACTGATTCA+TGG - chr7_3:35288560-35288579 Msa1102430:intron 45.0%
! GACTGATTCATGGCTTTCAC+CGG - chr7_3:35288570-35288589 Msa1102430:intron 45.0%
GTCTGCGGAAACATATGTTG+TGG + chr7_3:35288754-35288773 None:intergenic 45.0%
TTCCGCAGACACATTAGTGA+TGG - chr7_3:35288764-35288783 Msa1102430:intron 45.0%
! TTTTGATCGACTGACTGCGA+TGG + chr7_3:35288838-35288857 None:intergenic 45.0%
! TTATTCGGACTTACTGGCTG+TGG - chr7_3:35288951-35288970 Msa1102430:intron 45.0%
AAACTTTAGGCCGTGGTTCT+TGG + chr7_3:35289013-35289032 None:intergenic 45.0%
!! CTAGCTAGCTGGACTAGTTA+CGG - chr7_3:35289142-35289161 Msa1102430:intron 45.0%
! TTTTGCATGACAACTCTCGG+AGG - chr7_3:35289279-35289298 Msa1102430:intron 45.0%
AAGAGCTCCCATGATTCTGT+TGG - chr7_3:35287545-35287564 Msa1102430:three_prime_UTR 45.0%
AGAGCTCCCATGATTCTGTT+GGG - chr7_3:35287546-35287565 Msa1102430:three_prime_UTR 45.0%
! GTTGGTTCAAGATGTTACCC+AGG - chr7_3:35288116-35288135 Msa1102430:intron 45.0%
GTTCAAGATGTTACCCAGGT+TGG - chr7_3:35288120-35288139 Msa1102430:intron 45.0%
! TGTCATCTCGGACTGATTCA+TGG - chr7_3:35288560-35288579 Msa1102430:intron 45.0%
! GACTGATTCATGGCTTTCAC+CGG - chr7_3:35288570-35288589 Msa1102430:intron 45.0%
GTCTGCGGAAACATATGTTG+TGG + chr7_3:35288754-35288773 None:intergenic 45.0%
TTCCGCAGACACATTAGTGA+TGG - chr7_3:35288764-35288783 Msa1102430:intron 45.0%
! TTTTGATCGACTGACTGCGA+TGG + chr7_3:35288838-35288857 None:intergenic 45.0%
! TTATTCGGACTTACTGGCTG+TGG - chr7_3:35288951-35288970 Msa1102430:intron 45.0%
AAACTTTAGGCCGTGGTTCT+TGG + chr7_3:35289013-35289032 None:intergenic 45.0%
!! CTAGCTAGCTGGACTAGTTA+CGG - chr7_3:35289142-35289161 Msa1102430:intron 45.0%
!! AAAAATGTGGTGGTGGGGAG+TGG - chr7_3:35287435-35287454 Msa1102430:three_prime_UTR 50.0%
AGTTGTAGAAGGAGACTGGC+AGG - chr7_3:35287503-35287522 Msa1102430:three_prime_UTR 50.0%
GTTGTAGAAGGAGACTGGCA+GGG - chr7_3:35287504-35287523 Msa1102430:three_prime_UTR 50.0%
GTTGGGAGGAATTCCTCACA+AGG - chr7_3:35287563-35287582 Msa1102430:three_prime_UTR 50.0%
CATCTTACAGAGAGGTCAGC+CGG + chr7_3:35288592-35288611 None:intergenic 50.0%
CAGCACTGCATCTTACAGAG+AGG + chr7_3:35288600-35288619 None:intergenic 50.0%
!! ACCGAGCATCGATAATGGCT+AGG - chr7_3:35288644-35288663 Msa1102430:intron 50.0%
GCCTAGCCATTATCGATGCT+CGG + chr7_3:35288648-35288667 None:intergenic 50.0%
CCTTGTAATCTTCCACGGCA+TGG + chr7_3:35288705-35288724 None:intergenic 50.0%
CCATGCCGTGGAAGATTACA+AGG - chr7_3:35288702-35288721 Msa1102430:intron 50.0%
GTTTCTTATGGCACGGAGCT+TGG - chr7_3:35288786-35288805 Msa1102430:intron 50.0%
TATGGCACGGAGCTTGGATA+CGG - chr7_3:35288792-35288811 Msa1102430:intron 50.0%
!! GCTAGCATCCTGGTTCTAGA+AGG + chr7_3:35289127-35289146 None:intergenic 50.0%
!! AAAAATGTGGTGGTGGGGAG+TGG - chr7_3:35287435-35287454 Msa1102430:three_prime_UTR 50.0%
AGTTGTAGAAGGAGACTGGC+AGG - chr7_3:35287503-35287522 Msa1102430:three_prime_UTR 50.0%
GTTGTAGAAGGAGACTGGCA+GGG - chr7_3:35287504-35287523 Msa1102430:three_prime_UTR 50.0%
GTTGGGAGGAATTCCTCACA+AGG - chr7_3:35287563-35287582 Msa1102430:three_prime_UTR 50.0%
CATCTTACAGAGAGGTCAGC+CGG + chr7_3:35288592-35288611 None:intergenic 50.0%
CAGCACTGCATCTTACAGAG+AGG + chr7_3:35288600-35288619 None:intergenic 50.0%
!! ACCGAGCATCGATAATGGCT+AGG - chr7_3:35288644-35288663 Msa1102430:intron 50.0%
GCCTAGCCATTATCGATGCT+CGG + chr7_3:35288648-35288667 None:intergenic 50.0%
CCTTGTAATCTTCCACGGCA+TGG + chr7_3:35288705-35288724 None:intergenic 50.0%
CCATGCCGTGGAAGATTACA+AGG - chr7_3:35288702-35288721 Msa1102430:intron 50.0%
GTTTCTTATGGCACGGAGCT+TGG - chr7_3:35288786-35288805 Msa1102430:intron 50.0%
TATGGCACGGAGCTTGGATA+CGG - chr7_3:35288792-35288811 Msa1102430:intron 50.0%
!! GCTAGCATCCTGGTTCTAGA+AGG + chr7_3:35289127-35289146 None:intergenic 50.0%
! GTGGTGGGGAGTGGTTCTTT+AGG - chr7_3:35287444-35287463 Msa1102430:three_prime_UTR 55.0%
GCTCCCATGATTCTGTTGGG+AGG - chr7_3:35287549-35287568 Msa1102430:three_prime_UTR 55.0%
TCGACTGACTGCGATGGCTA+TGG + chr7_3:35288832-35288851 None:intergenic 55.0%
GAACCAGGATGCTAGCTAGC+TGG - chr7_3:35289131-35289150 Msa1102430:intron 55.0%
AGTCCAGCTAGCTAGCATCC+TGG + chr7_3:35289137-35289156 None:intergenic 55.0%
! GTGGTGGGGAGTGGTTCTTT+AGG - chr7_3:35287444-35287463 Msa1102430:three_prime_UTR 55.0%
GCTCCCATGATTCTGTTGGG+AGG - chr7_3:35287549-35287568 Msa1102430:three_prime_UTR 55.0%
TCGACTGACTGCGATGGCTA+TGG + chr7_3:35288832-35288851 None:intergenic 55.0%
GAACCAGGATGCTAGCTAGC+TGG - chr7_3:35289131-35289150 Msa1102430:intron 55.0%
AGTCCAGCTAGCTAGCATCC+TGG + chr7_3:35289137-35289156 None:intergenic 55.0%
GCATTTGCAGCACCATGCCG+TGG - chr7_3:35288690-35288709 Msa1102430:intron 60.0%
GCATTTGCAGCACCATGCCG+TGG - chr7_3:35288690-35288709 Msa1102430:intron 60.0%
Chromosome Type Strat End Strand Name
chr7_3 gene 35287294 35289298 35287294 ID=Msa1102430;Name=Msa1102430
chr7_3 mRNA 35287294 35289298 35287294 ID=Msa1102430-mRNA-1;Parent=Msa1102430;Name=Msa1102430-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|0.5|0.33|0.66|0|0.33|3|518|122
chr7_3 exon 35287294 35288053 35287294 ID=Msa1102430-mRNA-1:exon:7429;Parent=Msa1102430-mRNA-1
chr7_3 exon 35289008 35289126 35289008 ID=Msa1102430-mRNA-1:exon:7428;Parent=Msa1102430-mRNA-1
chr7_3 exon 35289291 35289298 35289291 ID=Msa1102430-mRNA-1:exon:7427;Parent=Msa1102430-mRNA-1
chr7_3 CDS 35289291 35289298 35289291 ID=Msa1102430-mRNA-1:cds;Parent=Msa1102430-mRNA-1
chr7_3 CDS 35289008 35289126 35289008 ID=Msa1102430-mRNA-1:cds;Parent=Msa1102430-mRNA-1
chr7_3 CDS 35287812 35288053 35287812 ID=Msa1102430-mRNA-1:cds;Parent=Msa1102430-mRNA-1
chr7_3 three_prime_UTR 35287294 35287811 35287294 ID=Msa1102430-mRNA-1:three_prime_utr;Parent=Msa1102430-mRNA-1
Gene Sequence

>Msa1102430

AGGCCAGGGTTCCATGTTGTGTCAGAATTTGATGATTCAAAAAGAAGTTGTAGAAGGAGACTGGCAGGGCACAATGAGAGAAGAAGAAAGAGCTCCCATGATTCTGTTGGGAGGAATTCCTCACAAGGTGGGTGTGCTTTCTCTCTTCTGTCATCTCGGACTGATTCATGGCTTTCACCGGCTGACCTCTCTGTAAGATGCAGTGCTGCATTAAGCGAATTGATAGCAGAAAACCGAGCATCGATAATGGCTAGGCAATACGTCTTAGACAGAGACTGGCATTTGCAGCACCATGCCGTGGAAGATTACAAGGAGATCCATTCCGAGTCCAATTATTTCCCACAACATATGTTTCCGCAGACACATTAG
Protein sequence

>Msa1102430

RPGFHVVSEFDDSKRSCRRRLAGHNERRRKSSHDSVGRNSSQGGCAFSLLSSRTDSWLSPADLSVRCSAALSELIAENRASIMARQYVLDRDWHLQHHAVEDYKEIHSESNYFPQHMFPQTH*