AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1105560


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1105560 MtrunA17_Chr7g0237771 97.753 89 2 0 1 89 1 89 2.02e-56 168
Msa1105560 MtrunA17_Chr8g0362131 50.746 67 32 1 10 76 15 80 3.29e-17 69.7
Msa1105560 MtrunA17_Chr3g0093001 50.794 63 31 0 12 74 17 79 5.07e-16 66.6
Msa1105560 MtrunA17_Chr5g0416431 46.970 66 35 0 11 76 16 81 5.67e-16 66.6
Msa1105560 MtrunA17_Chr7g0217141 43.836 73 41 0 2 74 6 78 1.55e-14 62.8
Msa1105560 MtrunA17_Chr6g0453041 49.206 63 32 0 12 74 15 77 1.86e-14 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1105560 AT1G26945.1 47.297 74 39 0 16 89 21 94 2.04e-17 70.5
Msa1105560 AT3G47710.1 52.703 74 34 1 2 74 6 79 4.90e-17 69.7
Msa1105560 AT3G28857.1 47.945 73 37 1 3 74 6 78 7.00e-16 66.6
Msa1105560 AT5G39860.1 50.769 65 31 1 11 74 14 78 5.67e-15 64.3
Msa1105560 AT5G15160.1 53.226 62 28 1 16 76 20 81 1.08e-13 61.2
Msa1105560 AT1G74500.1 48.438 64 32 1 12 74 17 80 1.29e-13 60.8

Find 26 sgRNAs with CRISPR-Local

Find 140 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGGAAACATTGGATCATATT+AGG 0.284722 7_3:-44116816 Msa1105560:CDS
TAGCCACATCACAATCTATA+AGG 0.298146 7_3:+44116609 None:intergenic
AATAGACTGTAGAATTAAAA+AGG 0.310346 7_3:+44116670 None:intergenic
ATACCCTTGAGTTGTTTCTT+TGG 0.331022 7_3:+44118327 None:intergenic
CAATGTTTCCTGCAAGATCT+TGG 0.360132 7_3:+44116828 None:intergenic
TTGAATCTCTTGAGTTTCTC+TGG 0.382021 7_3:+44118414 None:intergenic
TGAGAGGCTAACACAATTGA+TGG 0.413964 7_3:-44116764 Msa1105560:CDS
ATCAGCCTCCAAGATCTTGC+AGG 0.440449 7_3:-44116836 Msa1105560:CDS
CTATAAGGGAAGGAAACATA+AGG 0.484046 7_3:+44116624 None:intergenic
CAAGATCTTGCAGGAAACAT+TGG 0.497669 7_3:-44116827 Msa1105560:CDS
AGCCACATCACAATCTATAA+GGG 0.509284 7_3:+44116610 None:intergenic
TGTTTCCTGCAAGATCTTGG+AGG 0.509912 7_3:+44116831 None:intergenic
TATAAGGGAAGGAAACATAA+GGG 0.545314 7_3:+44116625 None:intergenic
ATATTTAATAATTAAGAACA+AGG 0.545635 7_3:-44116549 Msa1105560:three_prime_UTR
TATTAGGAGGCTTCAGAAAG+AGG 0.545892 7_3:-44116800 Msa1105560:CDS
TTGTTTCTTTGGTTCAGCTG+AGG 0.545919 7_3:+44118338 None:intergenic
TTCCCTTATAGATTGTGATG+TGG 0.561042 7_3:-44116612 Msa1105560:three_prime_UTR
TATACATACATATACATGAT+GGG 0.581615 7_3:+44116582 None:intergenic
AAACATTGGATCATATTAGG+AGG 0.601405 7_3:-44116813 Msa1105560:CDS
ATATACATACATATACATGA+TGG 0.604457 7_3:+44116581 None:intergenic
ACATCACAATCTATAAGGGA+AGG 0.609189 7_3:+44116614 None:intergenic
TAGGAGGCTTCAGAAAGAGG+TGG 0.622036 7_3:-44116797 Msa1105560:CDS
TGAACCAAAGAAACAACTCA+AGG 0.632348 7_3:-44118331 Msa1105560:CDS
AGGTGGAAGATCTCAGTGAG+AGG 0.651028 7_3:-44116780 Msa1105560:CDS
AGATTAATGATCTTGTGTCA+AGG 0.656005 7_3:-44118376 Msa1105560:CDS
GAACCAAAGAAACAACTCAA+GGG 0.668866 7_3:-44118330 Msa1105560:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATATATACAAAAGTAAAAT+TGG + chr7_3:44116830-44116849 None:intergenic 10.0%
!! ATATTTAATAATTAAGAACA+AGG - chr7_3:44118471-44118490 Msa1105560:five_prime_UTR 10.0%
!! TATATATACAAAAGTAAAAT+TGG + chr7_3:44116830-44116849 None:intergenic 10.0%
!! ATATTTAATAATTAAGAACA+AGG - chr7_3:44118471-44118490 Msa1105560:five_prime_UTR 10.0%
!! ATGAAAAATAAACTTTAAGT+TGG + chr7_3:44116935-44116954 None:intergenic 15.0%
!! AAATCATATTATAGTAACAT+AGG + chr7_3:44116958-44116977 None:intergenic 15.0%
!!! TAAGAAAATAATTTTACCTT+TGG - chr7_3:44117056-44117075 Msa1105560:intron 15.0%
!! TATATGAATCATACAATTTA+TGG + chr7_3:44117169-44117188 None:intergenic 15.0%
!! TTAATTATAACACAATTCAA+AGG + chr7_3:44117246-44117265 None:intergenic 15.0%
!!! GTTTTTTTTTTTTTAAGGAA+AGG + chr7_3:44117734-44117753 None:intergenic 15.0%
!!! TAAAGTGCTAAATATTATAT+AGG + chr7_3:44118030-44118049 None:intergenic 15.0%
!! TCTATGTTTAGATTAAAATT+TGG + chr7_3:44118086-44118105 None:intergenic 15.0%
!! ATGAAAAATAAACTTTAAGT+TGG + chr7_3:44116935-44116954 None:intergenic 15.0%
!! AAATCATATTATAGTAACAT+AGG + chr7_3:44116958-44116977 None:intergenic 15.0%
!!! TAAGAAAATAATTTTACCTT+TGG - chr7_3:44117056-44117075 Msa1105560:intron 15.0%
!! TATATGAATCATACAATTTA+TGG + chr7_3:44117169-44117188 None:intergenic 15.0%
!! TTAATTATAACACAATTCAA+AGG + chr7_3:44117246-44117265 None:intergenic 15.0%
!!! GTTTTTTTTTTTTTAAGGAA+AGG + chr7_3:44117734-44117753 None:intergenic 15.0%
!!! TAAAGTGCTAAATATTATAT+AGG + chr7_3:44118030-44118049 None:intergenic 15.0%
!! TCTATGTTTAGATTAAAATT+TGG + chr7_3:44118086-44118105 None:intergenic 15.0%
!!! TTTCTTTTGCTTACAATATT+TGG + chr7_3:44116561-44116580 None:intergenic 20.0%
!!! CATTTTAATAAATCTATGTG+TGG - chr7_3:44117600-44117619 Msa1105560:intron 20.0%
!!! TTCGAAACATATCTTTTTAT+TGG + chr7_3:44117661-44117680 None:intergenic 20.0%
!! CAGAAAAAGATATATAGTAT+AGG + chr7_3:44117984-44118003 None:intergenic 20.0%
!!! GTATATAACTCTAGAAAAAA+GGG + chr7_3:44118116-44118135 None:intergenic 20.0%
!!! AGTATATAACTCTAGAAAAA+AGG + chr7_3:44118117-44118136 None:intergenic 20.0%
!! AATAGACTGTAGAATTAAAA+AGG + chr7_3:44118353-44118372 None:intergenic 20.0%
!! TATACATACATATACATGAT+GGG + chr7_3:44118441-44118460 None:intergenic 20.0%
!! ATATACATACATATACATGA+TGG + chr7_3:44118442-44118461 None:intergenic 20.0%
!!! TTTCTTTTGCTTACAATATT+TGG + chr7_3:44116561-44116580 None:intergenic 20.0%
!!! CATTTTAATAAATCTATGTG+TGG - chr7_3:44117600-44117619 Msa1105560:intron 20.0%
!!! TTCGAAACATATCTTTTTAT+TGG + chr7_3:44117661-44117680 None:intergenic 20.0%
!! CAGAAAAAGATATATAGTAT+AGG + chr7_3:44117984-44118003 None:intergenic 20.0%
!!! GTATATAACTCTAGAAAAAA+GGG + chr7_3:44118116-44118135 None:intergenic 20.0%
!!! AGTATATAACTCTAGAAAAA+AGG + chr7_3:44118117-44118136 None:intergenic 20.0%
!! AATAGACTGTAGAATTAAAA+AGG + chr7_3:44118353-44118372 None:intergenic 20.0%
!! TATACATACATATACATGAT+GGG + chr7_3:44118441-44118460 None:intergenic 20.0%
!! ATATACATACATATACATGA+TGG + chr7_3:44118442-44118461 None:intergenic 20.0%
!! ATATGAATTCACTAGTTTCT+TGG + chr7_3:44116736-44116755 None:intergenic 25.0%
! TCTCAAAGTAAAATTGGTAA+TGG + chr7_3:44116804-44116823 None:intergenic 25.0%
!! TGGTTTTCTCAAAGTAAAAT+TGG + chr7_3:44116810-44116829 None:intergenic 25.0%
!! TGTCATTTAATACAAACCAA+AGG + chr7_3:44117075-44117094 None:intergenic 25.0%
!!! TTATGGTTTTGTAACATTCA+TGG + chr7_3:44117152-44117171 None:intergenic 25.0%
! ATACAAGTTTAGATTGCAAT+TGG - chr7_3:44117266-44117285 Msa1105560:intron 25.0%
!!! TTGTTTCTACAAACTGATTT+TGG - chr7_3:44117338-44117357 Msa1105560:intron 25.0%
!! GTCACACTAACAATTTTAAA+GGG + chr7_3:44117962-44117981 None:intergenic 25.0%
!! TTTTCTGTCAAAGATATAGT+AGG - chr7_3:44117997-44118016 Msa1105560:intron 25.0%
!! ATATGAATTCACTAGTTTCT+TGG + chr7_3:44116736-44116755 None:intergenic 25.0%
! TCTCAAAGTAAAATTGGTAA+TGG + chr7_3:44116804-44116823 None:intergenic 25.0%
!! TGGTTTTCTCAAAGTAAAAT+TGG + chr7_3:44116810-44116829 None:intergenic 25.0%
!! TGTCATTTAATACAAACCAA+AGG + chr7_3:44117075-44117094 None:intergenic 25.0%
!!! TTATGGTTTTGTAACATTCA+TGG + chr7_3:44117152-44117171 None:intergenic 25.0%
! ATACAAGTTTAGATTGCAAT+TGG - chr7_3:44117266-44117285 Msa1105560:intron 25.0%
!!! TTGTTTCTACAAACTGATTT+TGG - chr7_3:44117338-44117357 Msa1105560:intron 25.0%
!! GTCACACTAACAATTTTAAA+GGG + chr7_3:44117962-44117981 None:intergenic 25.0%
!! TTTTCTGTCAAAGATATAGT+AGG - chr7_3:44117997-44118016 Msa1105560:intron 25.0%
AGATTAATGATCTTGTGTCA+AGG - chr7_3:44116644-44116663 Msa1105560:three_prime_UTR 30.0%
ATAGCTAGATAGACATATGA+TGG - chr7_3:44116847-44116866 Msa1105560:intron 30.0%
GTAAGTAAAAGACGACAATT+TGG - chr7_3:44117100-44117119 Msa1105560:intron 30.0%
! GGTCACACTAACAATTTTAA+AGG + chr7_3:44117963-44117982 None:intergenic 30.0%
AGGAAACATTGGATCATATT+AGG - chr7_3:44118204-44118223 Msa1105560:intron 30.0%
AAACATTGGATCATATTAGG+AGG - chr7_3:44118207-44118226 Msa1105560:intron 30.0%
TATAAGGGAAGGAAACATAA+GGG + chr7_3:44118398-44118417 None:intergenic 30.0%
AGATTAATGATCTTGTGTCA+AGG - chr7_3:44116644-44116663 Msa1105560:three_prime_UTR 30.0%
ATAGCTAGATAGACATATGA+TGG - chr7_3:44116847-44116866 Msa1105560:intron 30.0%
GTAAGTAAAAGACGACAATT+TGG - chr7_3:44117100-44117119 Msa1105560:intron 30.0%
! GGTCACACTAACAATTTTAA+AGG + chr7_3:44117963-44117982 None:intergenic 30.0%
AGGAAACATTGGATCATATT+AGG - chr7_3:44118204-44118223 Msa1105560:intron 30.0%
AAACATTGGATCATATTAGG+AGG - chr7_3:44118207-44118226 Msa1105560:intron 30.0%
TATAAGGGAAGGAAACATAA+GGG + chr7_3:44118398-44118417 None:intergenic 30.0%
TTGAATCTCTTGAGTTTCTC+TGG + chr7_3:44116609-44116628 None:intergenic 35.0%
TGAACCAAAGAAACAACTCA+AGG - chr7_3:44116689-44116708 Msa1105560:exon 35.0%
GAACCAAAGAAACAACTCAA+GGG - chr7_3:44116690-44116709 Msa1105560:exon 35.0%
!! ATACCCTTGAGTTGTTTCTT+TGG + chr7_3:44116696-44116715 None:intergenic 35.0%
GGCAGCATTGTGAAAAAAAT+TGG + chr7_3:44117383-44117402 None:intergenic 35.0%
AGTCGTATAAGCCAAACAAT+TGG + chr7_3:44117404-44117423 None:intergenic 35.0%
! ACATAGACCCGAAAGTATAA+AGG - chr7_3:44117764-44117783 Msa1105560:intron 35.0%
! CATAGACCCGAAAGTATAAA+GGG - chr7_3:44117765-44117784 Msa1105560:intron 35.0%
TCAGTACCCTTTATACTTTC+GGG + chr7_3:44117774-44117793 None:intergenic 35.0%
CTCAGTACCCTTTATACTTT+CGG + chr7_3:44117775-44117794 None:intergenic 35.0%
TAAGGGAGAAAAAGAGCAAT+AGG + chr7_3:44118381-44118400 None:intergenic 35.0%
CTATAAGGGAAGGAAACATA+AGG + chr7_3:44118399-44118418 None:intergenic 35.0%
ACATCACAATCTATAAGGGA+AGG + chr7_3:44118409-44118428 None:intergenic 35.0%
TTCCCTTATAGATTGTGATG+TGG - chr7_3:44118408-44118427 Msa1105560:CDS 35.0%
AGCCACATCACAATCTATAA+GGG + chr7_3:44118413-44118432 None:intergenic 35.0%
TAGCCACATCACAATCTATA+AGG + chr7_3:44118414-44118433 None:intergenic 35.0%
TTGAATCTCTTGAGTTTCTC+TGG + chr7_3:44116609-44116628 None:intergenic 35.0%
TGAACCAAAGAAACAACTCA+AGG - chr7_3:44116689-44116708 Msa1105560:exon 35.0%
GAACCAAAGAAACAACTCAA+GGG - chr7_3:44116690-44116709 Msa1105560:exon 35.0%
!! ATACCCTTGAGTTGTTTCTT+TGG + chr7_3:44116696-44116715 None:intergenic 35.0%
GGCAGCATTGTGAAAAAAAT+TGG + chr7_3:44117383-44117402 None:intergenic 35.0%
AGTCGTATAAGCCAAACAAT+TGG + chr7_3:44117404-44117423 None:intergenic 35.0%
! ACATAGACCCGAAAGTATAA+AGG - chr7_3:44117764-44117783 Msa1105560:intron 35.0%
! CATAGACCCGAAAGTATAAA+GGG - chr7_3:44117765-44117784 Msa1105560:intron 35.0%
TCAGTACCCTTTATACTTTC+GGG + chr7_3:44117774-44117793 None:intergenic 35.0%
CTCAGTACCCTTTATACTTT+CGG + chr7_3:44117775-44117794 None:intergenic 35.0%
TAAGGGAGAAAAAGAGCAAT+AGG + chr7_3:44118381-44118400 None:intergenic 35.0%
CTATAAGGGAAGGAAACATA+AGG + chr7_3:44118399-44118418 None:intergenic 35.0%
ACATCACAATCTATAAGGGA+AGG + chr7_3:44118409-44118428 None:intergenic 35.0%
TTCCCTTATAGATTGTGATG+TGG - chr7_3:44118408-44118427 Msa1105560:CDS 35.0%
AGCCACATCACAATCTATAA+GGG + chr7_3:44118413-44118432 None:intergenic 35.0%
TAGCCACATCACAATCTATA+AGG + chr7_3:44118414-44118433 None:intergenic 35.0%
! TTGTTTCTTTGGTTCAGCTG+AGG + chr7_3:44116685-44116704 None:intergenic 40.0%
! TTGGACCTGCACTAAAGTTT+TGG - chr7_3:44117119-44117138 Msa1105560:intron 40.0%
TCACAATGCTGCCAATTGTT+TGG - chr7_3:44117390-44117409 Msa1105560:intron 40.0%
! AAAGAAGACCTCTCTAGAGA+CGG - chr7_3:44117506-44117525 Msa1105560:intron 40.0%
! TAATTCTTCCGTCTCTAGAG+AGG + chr7_3:44117517-44117536 None:intergenic 40.0%
TGCGTTACTAACACTCTTCA+TGG - chr7_3:44117685-44117704 Msa1105560:intron 40.0%
CAATGTTTCCTGCAAGATCT+TGG + chr7_3:44118195-44118214 None:intergenic 40.0%
CAAGATCTTGCAGGAAACAT+TGG - chr7_3:44118193-44118212 Msa1105560:intron 40.0%
TATTAGGAGGCTTCAGAAAG+AGG - chr7_3:44118220-44118239 Msa1105560:intron 40.0%
TGAGAGGCTAACACAATTGA+TGG - chr7_3:44118256-44118275 Msa1105560:intron 40.0%
! TTGTTTCTTTGGTTCAGCTG+AGG + chr7_3:44116685-44116704 None:intergenic 40.0%
! TTGGACCTGCACTAAAGTTT+TGG - chr7_3:44117119-44117138 Msa1105560:intron 40.0%
TCACAATGCTGCCAATTGTT+TGG - chr7_3:44117390-44117409 Msa1105560:intron 40.0%
! AAAGAAGACCTCTCTAGAGA+CGG - chr7_3:44117506-44117525 Msa1105560:intron 40.0%
! TAATTCTTCCGTCTCTAGAG+AGG + chr7_3:44117517-44117536 None:intergenic 40.0%
TGCGTTACTAACACTCTTCA+TGG - chr7_3:44117685-44117704 Msa1105560:intron 40.0%
CAATGTTTCCTGCAAGATCT+TGG + chr7_3:44118195-44118214 None:intergenic 40.0%
CAAGATCTTGCAGGAAACAT+TGG - chr7_3:44118193-44118212 Msa1105560:intron 40.0%
TATTAGGAGGCTTCAGAAAG+AGG - chr7_3:44118220-44118239 Msa1105560:intron 40.0%
TGAGAGGCTAACACAATTGA+TGG - chr7_3:44118256-44118275 Msa1105560:intron 40.0%
CCAAGCCAAAACTTTAGTGC+AGG + chr7_3:44117127-44117146 None:intergenic 45.0%
!!! CCTGCACTAAAGTTTTGGCT+TGG - chr7_3:44117124-44117143 Msa1105560:intron 45.0%
! GTCTCTAGAGAGGTCTTCTT+TGG + chr7_3:44117507-44117526 None:intergenic 45.0%
TGTTTCCTGCAAGATCTTGG+AGG + chr7_3:44118192-44118211 None:intergenic 45.0%
CCAAGCCAAAACTTTAGTGC+AGG + chr7_3:44117127-44117146 None:intergenic 45.0%
!!! CCTGCACTAAAGTTTTGGCT+TGG - chr7_3:44117124-44117143 Msa1105560:intron 45.0%
! GTCTCTAGAGAGGTCTTCTT+TGG + chr7_3:44117507-44117526 None:intergenic 45.0%
TGTTTCCTGCAAGATCTTGG+AGG + chr7_3:44118192-44118211 None:intergenic 45.0%
!!! TATTTTAAAAAAAAATACTT+AGG - chr7_3:44116900-44116919 Msa1105560:intron 5.0%
!!! TTTTTGTTTTTTTTTTTTTA+AGG + chr7_3:44117739-44117758 None:intergenic 5.0%
!!! TATTTTAAAAAAAAATACTT+AGG - chr7_3:44116900-44116919 Msa1105560:intron 5.0%
!!! TTTTTGTTTTTTTTTTTTTA+AGG + chr7_3:44117739-44117758 None:intergenic 5.0%
ATCAGCCTCCAAGATCTTGC+AGG - chr7_3:44118184-44118203 Msa1105560:intron 50.0%
TAGGAGGCTTCAGAAAGAGG+TGG - chr7_3:44118223-44118242 Msa1105560:intron 50.0%
AGGTGGAAGATCTCAGTGAG+AGG - chr7_3:44118240-44118259 Msa1105560:intron 50.0%
ATCAGCCTCCAAGATCTTGC+AGG - chr7_3:44118184-44118203 Msa1105560:intron 50.0%
TAGGAGGCTTCAGAAAGAGG+TGG - chr7_3:44118223-44118242 Msa1105560:intron 50.0%
AGGTGGAAGATCTCAGTGAG+AGG - chr7_3:44118240-44118259 Msa1105560:intron 50.0%
Chromosome Type Strat End Strand Name
chr7_3 gene 44116543 44118499 44116543 ID=Msa1105560;Name=Msa1105560
chr7_3 mRNA 44116543 44118499 44116543 ID=Msa1105560-mRNA-1;Parent=Msa1105560;Name=Msa1105560-mRNA-1;_AED=0.00;_eAED=0.00;_QI=55|1|1|1|1|1|2|162|89
chr7_3 exon 44116543 44116860 44116543 ID=Msa1105560-mRNA-1:exon:9113;Parent=Msa1105560-mRNA-1
chr7_3 exon 44118331 44118499 44118331 ID=Msa1105560-mRNA-1:exon:9112;Parent=Msa1105560-mRNA-1
chr7_3 five_prime_UTR 44118445 44118499 44118445 ID=Msa1105560-mRNA-1:five_prime_utr;Parent=Msa1105560-mRNA-1
chr7_3 CDS 44118331 44118444 44118331 ID=Msa1105560-mRNA-1:cds;Parent=Msa1105560-mRNA-1
chr7_3 CDS 44116705 44116860 44116705 ID=Msa1105560-mRNA-1:cds;Parent=Msa1105560-mRNA-1
chr7_3 three_prime_UTR 44116543 44116704 44116543 ID=Msa1105560-mRNA-1:three_prime_utr;Parent=Msa1105560-mRNA-1
Gene Sequence

>Msa1105560

ATGTCTAGCCAGAGAAACTCAAGAGATTCAAAGTTGAAAGAGAAACAGATTAATGATCTTGTGTCAAGGTTACAACTACTATTGCCTCAGCTGAACCAAAGAAACAACTCAAGGCAATCAGCCTCCAAGATCTTGCAGGAAACATTGGATCATATTAGGAGGCTTCAGAAAGAGGTGGAAGATCTCAGTGAGAGGCTAACACAATTGATGGATTCTGCAGATATCAATGATAATGACAGAAGAACTTTAGAGAACTTTTTTCAATATTAA

Protein sequence

>Msa1105560

MSSQRNSRDSKLKEKQINDLVSRLQLLLPQLNQRNNSRQSASKILQETLDHIRRLQKEVEDLSERLTQLMDSADINDNDRRTLENFFQY*