AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1148940


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1148940 MtrunA17_Chr7g0237771 96.629 89 3 0 1 89 1 89 4.90e-56 167
Msa1148940 MtrunA17_Chr8g0362131 50.746 67 32 1 10 76 15 80 2.98e-17 69.7
Msa1148940 MtrunA17_Chr3g0093001 50.794 63 31 0 12 74 17 79 4.40e-16 67.0
Msa1148940 MtrunA17_Chr5g0416431 46.970 66 35 0 11 76 16 81 5.49e-16 66.6
Msa1148940 MtrunA17_Chr6g0453041 49.206 63 32 0 12 74 15 77 1.67e-14 62.8
Msa1148940 MtrunA17_Chr7g0217141 43.836 73 41 0 2 74 6 78 2.11e-14 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1148940 AT1G26945.1 47.297 74 39 0 16 89 21 94 1.60e-17 70.9
Msa1148940 AT3G47710.1 53.521 71 32 1 5 74 9 79 6.29e-17 69.3
Msa1148940 AT3G28857.1 47.436 78 37 2 1 74 1 78 1.19e-15 66.2
Msa1148940 AT5G39860.1 50.769 65 31 1 11 74 14 78 6.18e-15 64.3
Msa1148940 AT5G15160.1 53.226 62 28 1 16 76 20 81 1.10e-13 61.2
Msa1148940 AT1G74500.1 48.438 64 32 1 12 74 17 80 1.14e-13 61.2

Find 26 sgRNAs with CRISPR-Local

Find 140 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGGAAACATTGGATCATATT+AGG 0.284722 7_4:-29578936 Msa1148940:CDS
TAGCCACATCACAATCAATA+AGG 0.310279 7_4:+29578747 None:intergenic
AATAGATTGTAGAATTAAAA+AGG 0.313928 7_4:+29578790 None:intergenic
ATACCCTTGAGTTGTTTCTT+TGG 0.331022 7_4:+29580465 None:intergenic
CAATGTTTCCTGCAAGATCT+TGG 0.360132 7_4:+29578948 None:intergenic
TTGAATCTCTTGAGTTTCTC+TGG 0.365643 7_4:+29580552 None:intergenic
TGAGAGGCTAACACAATTGA+TGG 0.413964 7_4:-29578884 Msa1148940:CDS
ATCAGCCTCCAAGATCTTGC+AGG 0.440449 7_4:-29578956 Msa1148940:CDS
CAAGATCTTGCAGGAAACAT+TGG 0.497669 7_4:-29578947 Msa1148940:CDS
TGTTTCCTGCAAGATCTTGG+AGG 0.509912 7_4:+29578951 None:intergenic
TTCCCTTATTGATTGTGATG+TGG 0.517223 7_4:-29578750 Msa1148940:three_prime_UTR
AGCCACATCACAATCAATAA+GGG 0.527392 7_4:+29578748 None:intergenic
TAAGAAAAGAGAGTCATGTC+TGG 0.537278 7_4:-29580575 Msa1148940:exon
CAATAAGGGAAGGAAACATA+AGG 0.545153 7_4:+29578762 None:intergenic
ATATTTAATAATTAAGAACA+AGG 0.545635 7_4:-29578687 Msa1148940:three_prime_UTR
TATTAGGAGGCTTCAGAAAG+AGG 0.545892 7_4:-29578920 Msa1148940:CDS
TTGTTTCTTTGGTTCAGCTG+AGG 0.545919 7_4:+29580476 None:intergenic
TATACATACATATACATGAT+GGG 0.579176 7_4:+29578720 None:intergenic
AAACATTGGATCATATTAGG+AGG 0.601405 7_4:-29578933 Msa1148940:CDS
ATATACATACATATACATGA+TGG 0.602018 7_4:+29578719 None:intergenic
TAGGAGGCTTCAGAAAGAGG+TGG 0.622036 7_4:-29578917 Msa1148940:CDS
TGAACCAAAGAAACAACTCA+AGG 0.632348 7_4:-29580469 Msa1148940:CDS
ACATCACAATCAATAAGGGA+AGG 0.638985 7_4:+29578752 None:intergenic
AGGTGGAAGATCTCAGTGAG+AGG 0.651028 7_4:-29578900 Msa1148940:CDS
AGATTAATGATCTTGTGTCA+AGG 0.656005 7_4:-29580514 Msa1148940:CDS
GAACCAAAGAAACAACTCAA+GGG 0.668866 7_4:-29580468 Msa1148940:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACTACAAAAAATATTATAAA+AGG - chr7_4:29578686-29578705 Msa1148940:three_prime_UTR 10.0%
!! TATATATACAAAAGTAAAAT+TGG + chr7_4:29578981-29579000 None:intergenic 10.0%
!!! TAAACATAATATCTTTTTAT+TGG + chr7_4:29579814-29579833 None:intergenic 10.0%
!! ATATTTAATAATTAAGAACA+AGG - chr7_4:29580622-29580641 Msa1148940:five_prime_UTR 10.0%
!! ACTACAAAAAATATTATAAA+AGG - chr7_4:29578686-29578705 Msa1148940:three_prime_UTR 10.0%
!! TATATATACAAAAGTAAAAT+TGG + chr7_4:29578981-29579000 None:intergenic 10.0%
!!! TAAACATAATATCTTTTTAT+TGG + chr7_4:29579814-29579833 None:intergenic 10.0%
!! ATATTTAATAATTAAGAACA+AGG - chr7_4:29580622-29580641 Msa1148940:five_prime_UTR 10.0%
!! ATGAAAAATAAACTTTAAGT+TGG + chr7_4:29579087-29579106 None:intergenic 15.0%
!! AAATCATATTATAGTAACAT+AGG + chr7_4:29579110-29579129 None:intergenic 15.0%
!!! ATAAGAAATAATTTTACCTT+TGG - chr7_4:29579211-29579230 Msa1148940:intron 15.0%
!! TATATGAATCATACAATTTA+TGG + chr7_4:29579324-29579343 None:intergenic 15.0%
!!! AGTAATGTAAGAATTTTAAA+GGG + chr7_4:29580138-29580157 None:intergenic 15.0%
!!! TAGTAATGTAAGAATTTTAA+AGG + chr7_4:29580139-29580158 None:intergenic 15.0%
!!! TAAAGTGCTAAATATTATAT+AGG + chr7_4:29580199-29580218 None:intergenic 15.0%
!! TCTATGTTTAGATTAAAATT+TGG + chr7_4:29580255-29580274 None:intergenic 15.0%
!! AATAGATTGTAGAATTAAAA+AGG + chr7_4:29580522-29580541 None:intergenic 15.0%
!! ATGAAAAATAAACTTTAAGT+TGG + chr7_4:29579087-29579106 None:intergenic 15.0%
!! AAATCATATTATAGTAACAT+AGG + chr7_4:29579110-29579129 None:intergenic 15.0%
!!! ATAAGAAATAATTTTACCTT+TGG - chr7_4:29579211-29579230 Msa1148940:intron 15.0%
!! TATATGAATCATACAATTTA+TGG + chr7_4:29579324-29579343 None:intergenic 15.0%
!!! AGTAATGTAAGAATTTTAAA+GGG + chr7_4:29580138-29580157 None:intergenic 15.0%
!!! TAGTAATGTAAGAATTTTAA+AGG + chr7_4:29580139-29580158 None:intergenic 15.0%
!!! TAAAGTGCTAAATATTATAT+AGG + chr7_4:29580199-29580218 None:intergenic 15.0%
!! TCTATGTTTAGATTAAAATT+TGG + chr7_4:29580255-29580274 None:intergenic 15.0%
!! AATAGATTGTAGAATTAAAA+AGG + chr7_4:29580522-29580541 None:intergenic 15.0%
!!! TTTCTTTTGCTTACAATATT+TGG + chr7_4:29578712-29578731 None:intergenic 20.0%
!! TTAATTAGAACACAATTCAA+AGG + chr7_4:29579401-29579420 None:intergenic 20.0%
!!! CATTTTAATAAATCTATGTG+TGG - chr7_4:29579753-29579772 Msa1148940:intron 20.0%
!!! TTCGAAAGATATCTTTTTAT+TGG + chr7_4:29579845-29579864 None:intergenic 20.0%
!!! GTTTTTGTGTTTTTTTTTGA+AGG + chr7_4:29579922-29579941 None:intergenic 20.0%
!!! GTATATAACTCTAGAAAAAA+GGG + chr7_4:29580285-29580304 None:intergenic 20.0%
!!! AGTATATAACTCTAGAAAAA+AGG + chr7_4:29580286-29580305 None:intergenic 20.0%
!! TATACATACATATACATGAT+GGG + chr7_4:29580592-29580611 None:intergenic 20.0%
!! ATATACATACATATACATGA+TGG + chr7_4:29580593-29580612 None:intergenic 20.0%
!!! TTTCTTTTGCTTACAATATT+TGG + chr7_4:29578712-29578731 None:intergenic 20.0%
!! TTAATTAGAACACAATTCAA+AGG + chr7_4:29579401-29579420 None:intergenic 20.0%
!!! CATTTTAATAAATCTATGTG+TGG - chr7_4:29579753-29579772 Msa1148940:intron 20.0%
!!! TTCGAAAGATATCTTTTTAT+TGG + chr7_4:29579845-29579864 None:intergenic 20.0%
!!! GTTTTTGTGTTTTTTTTTGA+AGG + chr7_4:29579922-29579941 None:intergenic 20.0%
!!! GTATATAACTCTAGAAAAAA+GGG + chr7_4:29580285-29580304 None:intergenic 20.0%
!!! AGTATATAACTCTAGAAAAA+AGG + chr7_4:29580286-29580305 None:intergenic 20.0%
!! TATACATACATATACATGAT+GGG + chr7_4:29580592-29580611 None:intergenic 20.0%
!! ATATACATACATATACATGA+TGG + chr7_4:29580593-29580612 None:intergenic 20.0%
!! ATATGAATTCACTAGTTTCT+TGG + chr7_4:29578887-29578906 None:intergenic 25.0%
! TCTCAAAGTAAAATTGGTAA+TGG + chr7_4:29578955-29578974 None:intergenic 25.0%
!! TGGTTTTCTCAAAGTAAAAT+TGG + chr7_4:29578961-29578980 None:intergenic 25.0%
!!! TTATGGTTTTGTAACATTCA+TGG + chr7_4:29579307-29579326 None:intergenic 25.0%
!!! TTGTTTCTACAAACTGATTT+TGG - chr7_4:29579493-29579512 Msa1148940:intron 25.0%
!!! TGTGTTTTTTTTTGAAGGAA+AGG + chr7_4:29579917-29579936 None:intergenic 25.0%
!! TTTTCTGTCAAAGATATAGT+AGG - chr7_4:29580166-29580185 Msa1148940:intron 25.0%
!! ATATGAATTCACTAGTTTCT+TGG + chr7_4:29578887-29578906 None:intergenic 25.0%
! TCTCAAAGTAAAATTGGTAA+TGG + chr7_4:29578955-29578974 None:intergenic 25.0%
!! TGGTTTTCTCAAAGTAAAAT+TGG + chr7_4:29578961-29578980 None:intergenic 25.0%
!!! TTATGGTTTTGTAACATTCA+TGG + chr7_4:29579307-29579326 None:intergenic 25.0%
!!! TTGTTTCTACAAACTGATTT+TGG - chr7_4:29579493-29579512 Msa1148940:intron 25.0%
!!! TGTGTTTTTTTTTGAAGGAA+AGG + chr7_4:29579917-29579936 None:intergenic 25.0%
!! TTTTCTGTCAAAGATATAGT+AGG - chr7_4:29580166-29580185 Msa1148940:intron 25.0%
AGATTAATGATCTTGTGTCA+AGG - chr7_4:29578795-29578814 Msa1148940:three_prime_UTR 30.0%
ATAGCTAGATAGACATATGA+TGG - chr7_4:29578998-29579017 Msa1148940:intron 30.0%
TGTCATGTAATACAAACCAA+AGG + chr7_4:29579230-29579249 None:intergenic 30.0%
GTAAGTAAAAGACGACAAAT+TGG - chr7_4:29579255-29579274 Msa1148940:intron 30.0%
AGGTGATAAACGTGATAAAT+AGG + chr7_4:29579381-29579400 None:intergenic 30.0%
AGGAAACATTGGATCATATT+AGG - chr7_4:29580373-29580392 Msa1148940:intron 30.0%
AAACATTGGATCATATTAGG+AGG - chr7_4:29580376-29580395 Msa1148940:intron 30.0%
AGATTAATGATCTTGTGTCA+AGG - chr7_4:29578795-29578814 Msa1148940:three_prime_UTR 30.0%
ATAGCTAGATAGACATATGA+TGG - chr7_4:29578998-29579017 Msa1148940:intron 30.0%
TGTCATGTAATACAAACCAA+AGG + chr7_4:29579230-29579249 None:intergenic 30.0%
GTAAGTAAAAGACGACAAAT+TGG - chr7_4:29579255-29579274 Msa1148940:intron 30.0%
AGGTGATAAACGTGATAAAT+AGG + chr7_4:29579381-29579400 None:intergenic 30.0%
AGGAAACATTGGATCATATT+AGG - chr7_4:29580373-29580392 Msa1148940:intron 30.0%
AAACATTGGATCATATTAGG+AGG - chr7_4:29580376-29580395 Msa1148940:intron 30.0%
TAAGAAAAGAGAGTCATGTC+TGG - chr7_4:29578734-29578753 Msa1148940:three_prime_UTR 35.0%
TTGAATCTCTTGAGTTTCTC+TGG + chr7_4:29578760-29578779 None:intergenic 35.0%
TGAACCAAAGAAACAACTCA+AGG - chr7_4:29578840-29578859 Msa1148940:CDS 35.0%
GAACCAAAGAAACAACTCAA+GGG - chr7_4:29578841-29578860 Msa1148940:CDS 35.0%
!! ATACCCTTGAGTTGTTTCTT+TGG + chr7_4:29578847-29578866 None:intergenic 35.0%
AGACGAGTTTAGATTGCAAT+TGG - chr7_4:29579421-29579440 Msa1148940:intron 35.0%
GGCAGCATTGTGAAAAAAAT+TGG + chr7_4:29579538-29579557 None:intergenic 35.0%
AGTCGTTTAAGCCAAACAAT+TGG + chr7_4:29579559-29579578 None:intergenic 35.0%
! ACATAGACCCGAAAGTATAA+AGG - chr7_4:29579940-29579959 Msa1148940:intron 35.0%
! CATAGACCCGAAAGTATAAA+GGG - chr7_4:29579941-29579960 Msa1148940:intron 35.0%
TCAGTACCCTTTATACTTTC+GGG + chr7_4:29579950-29579969 None:intergenic 35.0%
CTCAGTACCCTTTATACTTT+CGG + chr7_4:29579951-29579970 None:intergenic 35.0%
CAATAAGGGAAGGAAACATA+AGG + chr7_4:29580550-29580569 None:intergenic 35.0%
ACATCACAATCAATAAGGGA+AGG + chr7_4:29580560-29580579 None:intergenic 35.0%
! TTCCCTTATTGATTGTGATG+TGG - chr7_4:29580559-29580578 Msa1148940:CDS 35.0%
AGCCACATCACAATCAATAA+GGG + chr7_4:29580564-29580583 None:intergenic 35.0%
TAGCCACATCACAATCAATA+AGG + chr7_4:29580565-29580584 None:intergenic 35.0%
TAAGAAAAGAGAGTCATGTC+TGG - chr7_4:29578734-29578753 Msa1148940:three_prime_UTR 35.0%
TTGAATCTCTTGAGTTTCTC+TGG + chr7_4:29578760-29578779 None:intergenic 35.0%
TGAACCAAAGAAACAACTCA+AGG - chr7_4:29578840-29578859 Msa1148940:CDS 35.0%
GAACCAAAGAAACAACTCAA+GGG - chr7_4:29578841-29578860 Msa1148940:CDS 35.0%
!! ATACCCTTGAGTTGTTTCTT+TGG + chr7_4:29578847-29578866 None:intergenic 35.0%
AGACGAGTTTAGATTGCAAT+TGG - chr7_4:29579421-29579440 Msa1148940:intron 35.0%
GGCAGCATTGTGAAAAAAAT+TGG + chr7_4:29579538-29579557 None:intergenic 35.0%
AGTCGTTTAAGCCAAACAAT+TGG + chr7_4:29579559-29579578 None:intergenic 35.0%
! ACATAGACCCGAAAGTATAA+AGG - chr7_4:29579940-29579959 Msa1148940:intron 35.0%
! CATAGACCCGAAAGTATAAA+GGG - chr7_4:29579941-29579960 Msa1148940:intron 35.0%
TCAGTACCCTTTATACTTTC+GGG + chr7_4:29579950-29579969 None:intergenic 35.0%
CTCAGTACCCTTTATACTTT+CGG + chr7_4:29579951-29579970 None:intergenic 35.0%
CAATAAGGGAAGGAAACATA+AGG + chr7_4:29580550-29580569 None:intergenic 35.0%
ACATCACAATCAATAAGGGA+AGG + chr7_4:29580560-29580579 None:intergenic 35.0%
! TTCCCTTATTGATTGTGATG+TGG - chr7_4:29580559-29580578 Msa1148940:CDS 35.0%
AGCCACATCACAATCAATAA+GGG + chr7_4:29580564-29580583 None:intergenic 35.0%
TAGCCACATCACAATCAATA+AGG + chr7_4:29580565-29580584 None:intergenic 35.0%
! TTGTTTCTTTGGTTCAGCTG+AGG + chr7_4:29578836-29578855 None:intergenic 40.0%
! TTGGACCTGCACTAAAGTTT+TGG - chr7_4:29579274-29579293 Msa1148940:intron 40.0%
TCACAATGCTGCCAATTGTT+TGG - chr7_4:29579545-29579564 Msa1148940:intron 40.0%
! AAAGAAGACCTCTCTAGAGA+CGG - chr7_4:29579661-29579680 Msa1148940:intron 40.0%
! TAATTCTTCCGTCTCTAGAG+AGG + chr7_4:29579672-29579691 None:intergenic 40.0%
CAATGTTTCCTGCAAGATCT+TGG + chr7_4:29580364-29580383 None:intergenic 40.0%
CAAGATCTTGCAGGAAACAT+TGG - chr7_4:29580362-29580381 Msa1148940:intron 40.0%
TATTAGGAGGCTTCAGAAAG+AGG - chr7_4:29580389-29580408 Msa1148940:intron 40.0%
TGAGAGGCTAACACAATTGA+TGG - chr7_4:29580425-29580444 Msa1148940:intron 40.0%
! TTGTTTCTTTGGTTCAGCTG+AGG + chr7_4:29578836-29578855 None:intergenic 40.0%
! TTGGACCTGCACTAAAGTTT+TGG - chr7_4:29579274-29579293 Msa1148940:intron 40.0%
TCACAATGCTGCCAATTGTT+TGG - chr7_4:29579545-29579564 Msa1148940:intron 40.0%
! AAAGAAGACCTCTCTAGAGA+CGG - chr7_4:29579661-29579680 Msa1148940:intron 40.0%
! TAATTCTTCCGTCTCTAGAG+AGG + chr7_4:29579672-29579691 None:intergenic 40.0%
CAATGTTTCCTGCAAGATCT+TGG + chr7_4:29580364-29580383 None:intergenic 40.0%
CAAGATCTTGCAGGAAACAT+TGG - chr7_4:29580362-29580381 Msa1148940:intron 40.0%
TATTAGGAGGCTTCAGAAAG+AGG - chr7_4:29580389-29580408 Msa1148940:intron 40.0%
TGAGAGGCTAACACAATTGA+TGG - chr7_4:29580425-29580444 Msa1148940:intron 40.0%
CCAAGCCAAAACTTTAGTGC+AGG + chr7_4:29579282-29579301 None:intergenic 45.0%
!!! CCTGCACTAAAGTTTTGGCT+TGG - chr7_4:29579279-29579298 Msa1148940:intron 45.0%
! GTCTCTAGAGAGGTCTTCTT+TGG + chr7_4:29579662-29579681 None:intergenic 45.0%
TGTTTCCTGCAAGATCTTGG+AGG + chr7_4:29580361-29580380 None:intergenic 45.0%
CCAAGCCAAAACTTTAGTGC+AGG + chr7_4:29579282-29579301 None:intergenic 45.0%
!!! CCTGCACTAAAGTTTTGGCT+TGG - chr7_4:29579279-29579298 Msa1148940:intron 45.0%
! GTCTCTAGAGAGGTCTTCTT+TGG + chr7_4:29579662-29579681 None:intergenic 45.0%
TGTTTCCTGCAAGATCTTGG+AGG + chr7_4:29580361-29580380 None:intergenic 45.0%
!!! ATTTTTAAAAAAAAATACTT+AGG - chr7_4:29579052-29579071 Msa1148940:intron 5.0%
!!! ATTTTTAAAAAAAAATACTT+AGG - chr7_4:29579052-29579071 Msa1148940:intron 5.0%
ATCAGCCTCCAAGATCTTGC+AGG - chr7_4:29580353-29580372 Msa1148940:intron 50.0%
TAGGAGGCTTCAGAAAGAGG+TGG - chr7_4:29580392-29580411 Msa1148940:intron 50.0%
AGGTGGAAGATCTCAGTGAG+AGG - chr7_4:29580409-29580428 Msa1148940:intron 50.0%
ATCAGCCTCCAAGATCTTGC+AGG - chr7_4:29580353-29580372 Msa1148940:intron 50.0%
TAGGAGGCTTCAGAAAGAGG+TGG - chr7_4:29580392-29580411 Msa1148940:intron 50.0%
AGGTGGAAGATCTCAGTGAG+AGG - chr7_4:29580409-29580428 Msa1148940:intron 50.0%
Chromosome Type Strat End Strand Name
chr7_4 gene 29578681 29580650 29578681 ID=Msa1148940;Name=Msa1148940
chr7_4 mRNA 29578681 29580650 29578681 ID=Msa1148940-mRNA-1;Parent=Msa1148940;Name=Msa1148940-mRNA-1;_AED=0.00;_eAED=0.00;_QI=68|1|1|1|1|1|2|144|89
chr7_4 exon 29578681 29578980 29578681 ID=Msa1148940-mRNA-1:exon:7270;Parent=Msa1148940-mRNA-1
chr7_4 exon 29580469 29580650 29580469 ID=Msa1148940-mRNA-1:exon:7269;Parent=Msa1148940-mRNA-1
chr7_4 five_prime_UTR 29580583 29580650 29580583 ID=Msa1148940-mRNA-1:five_prime_utr;Parent=Msa1148940-mRNA-1
chr7_4 CDS 29580469 29580582 29580469 ID=Msa1148940-mRNA-1:cds;Parent=Msa1148940-mRNA-1
chr7_4 CDS 29578825 29578980 29578825 ID=Msa1148940-mRNA-1:cds;Parent=Msa1148940-mRNA-1
chr7_4 three_prime_UTR 29578681 29578824 29578681 ID=Msa1148940-mRNA-1:three_prime_utr;Parent=Msa1148940-mRNA-1
Gene Sequence

>Msa1148940

ATGTCTGGCCAGAGAAACTCAAGAGATTCAAAGTTGAAAGAGAAACAGATTAATGATCTTGTGTCAAGGTTACAACTACTATTGCCTCAGCTGAACCAAAGAAACAACTCAAGGCAATCAGCCTCCAAGATCTTGCAGGAAACATTGGATCATATTAGGAGGCTTCAGAAAGAGGTGGAAGATCTCAGTGAGAGGCTAACACAATTGATGGATTCTGCAGATATCAATGATAATGACAGAAGAACTTTAGAGAACTTTTTTCAATATTAA

Protein sequence

>Msa1148940

MSGQRNSRDSKLKEKQINDLVSRLQLLLPQLNQRNNSRQSASKILQETLDHIRRLQKEVEDLSERLTQLMDSADINDNDRRTLENFFQY*