AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1021570


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1021570 MtrunA17_Chr7g0237771 96.629 89 3 0 1 89 1 89 4.90e-56 167
Msa1021570 MtrunA17_Chr8g0362131 50.746 67 32 1 10 76 15 80 2.98e-17 69.7
Msa1021570 MtrunA17_Chr3g0093001 50.794 63 31 0 12 74 17 79 4.40e-16 67.0
Msa1021570 MtrunA17_Chr5g0416431 46.970 66 35 0 11 76 16 81 5.49e-16 66.6
Msa1021570 MtrunA17_Chr6g0453041 49.206 63 32 0 12 74 15 77 1.67e-14 62.8
Msa1021570 MtrunA17_Chr7g0217141 43.836 73 41 0 2 74 6 78 2.11e-14 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1021570 AT1G26945.1 47.297 74 39 0 16 89 21 94 1.60e-17 70.9
Msa1021570 AT3G47710.1 53.521 71 32 1 5 74 9 79 6.29e-17 69.3
Msa1021570 AT3G28857.1 47.436 78 37 2 1 74 1 78 1.19e-15 66.2
Msa1021570 AT5G39860.1 50.769 65 31 1 11 74 14 78 6.18e-15 64.3
Msa1021570 AT5G15160.1 53.226 62 28 1 16 76 20 81 1.10e-13 61.2
Msa1021570 AT1G74500.1 48.438 64 32 1 12 74 17 80 1.14e-13 61.2

Find 27 sgRNAs with CRISPR-Local

Find 140 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGGAAACATTGGATCATATT+AGG 0.284722 7_1:-46003898 Msa1021570:CDS
TAGCCACATCACAATCAATA+AGG 0.310279 7_1:+46003691 None:intergenic
AATAGACTGTAGAATTAAAA+AGG 0.310346 7_1:+46003752 None:intergenic
ATACCCTTGAGTTGTTTCTT+TGG 0.331022 7_1:+46005437 None:intergenic
CAATGTTTCCTGCAAGATCT+TGG 0.360132 7_1:+46003910 None:intergenic
TTGAATCTCTTGAGTTTCTC+TGG 0.365643 7_1:+46005524 None:intergenic
TGAGAGGCTAACACAATTGA+TGG 0.413964 7_1:-46003846 Msa1021570:CDS
ATCAGCCTCCAAGATCTTGC+AGG 0.440449 7_1:-46003918 Msa1021570:CDS
CAATAAGGGAAGGAAACATA+AGG 0.473262 7_1:+46003706 None:intergenic
CAAGATCTTGCAGGAAACAT+TGG 0.497669 7_1:-46003909 Msa1021570:CDS
AGCCACATCACAATCAATAA+GGG 0.500226 7_1:+46003692 None:intergenic
TGTTTCCTGCAAGATCTTGG+AGG 0.509912 7_1:+46003913 None:intergenic
TTCCCTTATTGATTGTGATG+TGG 0.517223 7_1:-46003694 Msa1021570:three_prime_UTR
TAAGAAAAGAGAGTCATGTC+TGG 0.537278 7_1:-46005547 Msa1021570:exon
AATAAGGGAAGGAAACATAA+GGG 0.545314 7_1:+46003707 None:intergenic
ATATTTAATAATTAAGAACA+AGG 0.545635 7_1:-46003631 Msa1021570:three_prime_UTR
TATTAGGAGGCTTCAGAAAG+AGG 0.545892 7_1:-46003882 Msa1021570:CDS
TTGTTTCTTTGGTTCAGCTG+AGG 0.545919 7_1:+46005448 None:intergenic
TATACATACATATACATGAT+GGG 0.581615 7_1:+46003664 None:intergenic
AAACATTGGATCATATTAGG+AGG 0.601405 7_1:-46003895 Msa1021570:CDS
ATATACATACATATACATGA+TGG 0.604457 7_1:+46003663 None:intergenic
TAGGAGGCTTCAGAAAGAGG+TGG 0.622036 7_1:-46003879 Msa1021570:CDS
TGAACCAAAGAAACAACTCA+AGG 0.632348 7_1:-46005441 Msa1021570:CDS
ACATCACAATCAATAAGGGA+AGG 0.638985 7_1:+46003696 None:intergenic
AGGTGGAAGATCTCAGTGAG+AGG 0.651028 7_1:-46003862 Msa1021570:CDS
AGATTAATGATCTTGTGTCA+AGG 0.656005 7_1:-46005486 Msa1021570:CDS
GAACCAAAGAAACAACTCAA+GGG 0.668866 7_1:-46005440 Msa1021570:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATATATACAAAAGTAAAAT+TGG + chr7_1:46003912-46003931 None:intergenic 10.0%
!!! ATGTTTTTTTTTTTTTTTGA+AGG + chr7_1:46004823-46004842 None:intergenic 10.0%
!! ATATTTAATAATTAAGAACA+AGG - chr7_1:46005581-46005600 Msa1021570:five_prime_UTR 10.0%
!! TATATATACAAAAGTAAAAT+TGG + chr7_1:46003912-46003931 None:intergenic 10.0%
!!! ATGTTTTTTTTTTTTTTTGA+AGG + chr7_1:46004823-46004842 None:intergenic 10.0%
!! ATATTTAATAATTAAGAACA+AGG - chr7_1:46005581-46005600 Msa1021570:five_prime_UTR 10.0%
!! ATGAAAAATAAACTTTAAGT+TGG + chr7_1:46004017-46004036 None:intergenic 15.0%
!! AAATCATATTATAGTAACAT+AGG + chr7_1:46004040-46004059 None:intergenic 15.0%
!!! TAAGAAAATAATTTTACCTT+TGG - chr7_1:46004142-46004161 Msa1021570:intron 15.0%
!! TATATGAATCATACAATTTA+TGG + chr7_1:46004255-46004274 None:intergenic 15.0%
!! TTAATTATAACACAATTCAA+AGG + chr7_1:46004332-46004351 None:intergenic 15.0%
!!! TTTTTTTTTTTTTGAAGGAA+AGG + chr7_1:46004818-46004837 None:intergenic 15.0%
!!! TAAAGTGCTAAATATTATAT+AGG + chr7_1:46005111-46005130 None:intergenic 15.0%
!! TCTATGTTTAGATTAAAATT+TGG + chr7_1:46005167-46005186 None:intergenic 15.0%
!! ATGAAAAATAAACTTTAAGT+TGG + chr7_1:46004017-46004036 None:intergenic 15.0%
!! AAATCATATTATAGTAACAT+AGG + chr7_1:46004040-46004059 None:intergenic 15.0%
!!! TAAGAAAATAATTTTACCTT+TGG - chr7_1:46004142-46004161 Msa1021570:intron 15.0%
!! TATATGAATCATACAATTTA+TGG + chr7_1:46004255-46004274 None:intergenic 15.0%
!! TTAATTATAACACAATTCAA+AGG + chr7_1:46004332-46004351 None:intergenic 15.0%
!!! TTTTTTTTTTTTTGAAGGAA+AGG + chr7_1:46004818-46004837 None:intergenic 15.0%
!!! TAAAGTGCTAAATATTATAT+AGG + chr7_1:46005111-46005130 None:intergenic 15.0%
!! TCTATGTTTAGATTAAAATT+TGG + chr7_1:46005167-46005186 None:intergenic 15.0%
!!! TTTCTTTTGCTTACAATATT+TGG + chr7_1:46003643-46003662 None:intergenic 20.0%
!!! CATTTTAATAAATCTATGTG+TGG - chr7_1:46004684-46004703 Msa1021570:intron 20.0%
!!! TTCGAAACATATCTTTTTAT+TGG + chr7_1:46004745-46004764 None:intergenic 20.0%
!! CAGAAAAAGATATATAGTAT+AGG + chr7_1:46005065-46005084 None:intergenic 20.0%
!!! GTATATAACTCTAGAAAAAA+GGG + chr7_1:46005197-46005216 None:intergenic 20.0%
!!! AGTATATAACTCTAGAAAAA+AGG + chr7_1:46005198-46005217 None:intergenic 20.0%
!! AATAGACTGTAGAATTAAAA+AGG + chr7_1:46005463-46005482 None:intergenic 20.0%
!! TATACATACATATACATGAT+GGG + chr7_1:46005551-46005570 None:intergenic 20.0%
!! ATATACATACATATACATGA+TGG + chr7_1:46005552-46005571 None:intergenic 20.0%
!!! TTTCTTTTGCTTACAATATT+TGG + chr7_1:46003643-46003662 None:intergenic 20.0%
!!! CATTTTAATAAATCTATGTG+TGG - chr7_1:46004684-46004703 Msa1021570:intron 20.0%
!!! TTCGAAACATATCTTTTTAT+TGG + chr7_1:46004745-46004764 None:intergenic 20.0%
!! CAGAAAAAGATATATAGTAT+AGG + chr7_1:46005065-46005084 None:intergenic 20.0%
!!! GTATATAACTCTAGAAAAAA+GGG + chr7_1:46005197-46005216 None:intergenic 20.0%
!!! AGTATATAACTCTAGAAAAA+AGG + chr7_1:46005198-46005217 None:intergenic 20.0%
!! AATAGACTGTAGAATTAAAA+AGG + chr7_1:46005463-46005482 None:intergenic 20.0%
!! TATACATACATATACATGAT+GGG + chr7_1:46005551-46005570 None:intergenic 20.0%
!! ATATACATACATATACATGA+TGG + chr7_1:46005552-46005571 None:intergenic 20.0%
! TCTCAAAGTAAAATTGGTAA+TGG + chr7_1:46003886-46003905 None:intergenic 25.0%
!! TGGTTTTCTCAAAGTAAAAT+TGG + chr7_1:46003892-46003911 None:intergenic 25.0%
!! TGTCATTTAATACAAACCAA+AGG + chr7_1:46004161-46004180 None:intergenic 25.0%
!!! TTATGGTTTTGTAACATTCA+TGG + chr7_1:46004238-46004257 None:intergenic 25.0%
! ATACAAGTTTAGATTGCAAT+TGG - chr7_1:46004352-46004371 Msa1021570:intron 25.0%
!!! TTGTTTCTACAAACTGATTT+TGG - chr7_1:46004424-46004443 Msa1021570:intron 25.0%
!! GTCACACTAACAATTTTAAA+GGG + chr7_1:46005043-46005062 None:intergenic 25.0%
!! TTTTCTGTCAAAGATATAGT+AGG - chr7_1:46005078-46005097 Msa1021570:intron 25.0%
! TCTCAAAGTAAAATTGGTAA+TGG + chr7_1:46003886-46003905 None:intergenic 25.0%
!! TGGTTTTCTCAAAGTAAAAT+TGG + chr7_1:46003892-46003911 None:intergenic 25.0%
!! TGTCATTTAATACAAACCAA+AGG + chr7_1:46004161-46004180 None:intergenic 25.0%
!!! TTATGGTTTTGTAACATTCA+TGG + chr7_1:46004238-46004257 None:intergenic 25.0%
! ATACAAGTTTAGATTGCAAT+TGG - chr7_1:46004352-46004371 Msa1021570:intron 25.0%
!!! TTGTTTCTACAAACTGATTT+TGG - chr7_1:46004424-46004443 Msa1021570:intron 25.0%
!! GTCACACTAACAATTTTAAA+GGG + chr7_1:46005043-46005062 None:intergenic 25.0%
!! TTTTCTGTCAAAGATATAGT+AGG - chr7_1:46005078-46005097 Msa1021570:intron 25.0%
AGATTAATGATCTTGTGTCA+AGG - chr7_1:46003726-46003745 Msa1021570:three_prime_UTR 30.0%
! GTATGAATTCACTAGTTTCT+TGG + chr7_1:46003818-46003837 None:intergenic 30.0%
ATAGCTAGATAGACATATGA+TGG - chr7_1:46003929-46003948 Msa1021570:intron 30.0%
GTAAGTAAAAGACGACAATT+TGG - chr7_1:46004186-46004205 Msa1021570:intron 30.0%
! ATAGACCCGAAAGTATATAA+AGG - chr7_1:46004841-46004860 Msa1021570:intron 30.0%
! TAGACCCGAAAGTATATAAA+GGG - chr7_1:46004842-46004861 Msa1021570:intron 30.0%
AGTACCCTTTATATACTTTC+GGG + chr7_1:46004849-46004868 None:intergenic 30.0%
CAGTACCCTTTATATACTTT+CGG + chr7_1:46004850-46004869 None:intergenic 30.0%
! GGTCACACTAACAATTTTAA+AGG + chr7_1:46005044-46005063 None:intergenic 30.0%
AGGAAACATTGGATCATATT+AGG - chr7_1:46005314-46005333 Msa1021570:intron 30.0%
AAACATTGGATCATATTAGG+AGG - chr7_1:46005317-46005336 Msa1021570:intron 30.0%
AATAAGGGAAGGAAACATAA+GGG + chr7_1:46005508-46005527 None:intergenic 30.0%
AGATTAATGATCTTGTGTCA+AGG - chr7_1:46003726-46003745 Msa1021570:three_prime_UTR 30.0%
! GTATGAATTCACTAGTTTCT+TGG + chr7_1:46003818-46003837 None:intergenic 30.0%
ATAGCTAGATAGACATATGA+TGG - chr7_1:46003929-46003948 Msa1021570:intron 30.0%
GTAAGTAAAAGACGACAATT+TGG - chr7_1:46004186-46004205 Msa1021570:intron 30.0%
! ATAGACCCGAAAGTATATAA+AGG - chr7_1:46004841-46004860 Msa1021570:intron 30.0%
! TAGACCCGAAAGTATATAAA+GGG - chr7_1:46004842-46004861 Msa1021570:intron 30.0%
AGTACCCTTTATATACTTTC+GGG + chr7_1:46004849-46004868 None:intergenic 30.0%
CAGTACCCTTTATATACTTT+CGG + chr7_1:46004850-46004869 None:intergenic 30.0%
! GGTCACACTAACAATTTTAA+AGG + chr7_1:46005044-46005063 None:intergenic 30.0%
AGGAAACATTGGATCATATT+AGG - chr7_1:46005314-46005333 Msa1021570:intron 30.0%
AAACATTGGATCATATTAGG+AGG - chr7_1:46005317-46005336 Msa1021570:intron 30.0%
AATAAGGGAAGGAAACATAA+GGG + chr7_1:46005508-46005527 None:intergenic 30.0%
TAAGAAAAGAGAGTCATGTC+TGG - chr7_1:46003665-46003684 Msa1021570:three_prime_UTR 35.0%
TTGAATCTCTTGAGTTTCTC+TGG + chr7_1:46003691-46003710 None:intergenic 35.0%
TGAACCAAAGAAACAACTCA+AGG - chr7_1:46003771-46003790 Msa1021570:exon 35.0%
GAACCAAAGAAACAACTCAA+GGG - chr7_1:46003772-46003791 Msa1021570:exon 35.0%
!! ATACCCTTGAGTTGTTTCTT+TGG + chr7_1:46003778-46003797 None:intergenic 35.0%
GGCAGCATTGTGAAAAAAAT+TGG + chr7_1:46004469-46004488 None:intergenic 35.0%
AGTCGTATAAGCCAAACAAT+TGG + chr7_1:46004490-46004509 None:intergenic 35.0%
TAAGGGAGAAAAAGAGCAAT+AGG + chr7_1:46005491-46005510 None:intergenic 35.0%
CAATAAGGGAAGGAAACATA+AGG + chr7_1:46005509-46005528 None:intergenic 35.0%
ACATCACAATCAATAAGGGA+AGG + chr7_1:46005519-46005538 None:intergenic 35.0%
! TTCCCTTATTGATTGTGATG+TGG - chr7_1:46005518-46005537 Msa1021570:CDS 35.0%
AGCCACATCACAATCAATAA+GGG + chr7_1:46005523-46005542 None:intergenic 35.0%
TAGCCACATCACAATCAATA+AGG + chr7_1:46005524-46005543 None:intergenic 35.0%
TAAGAAAAGAGAGTCATGTC+TGG - chr7_1:46003665-46003684 Msa1021570:three_prime_UTR 35.0%
TTGAATCTCTTGAGTTTCTC+TGG + chr7_1:46003691-46003710 None:intergenic 35.0%
TGAACCAAAGAAACAACTCA+AGG - chr7_1:46003771-46003790 Msa1021570:exon 35.0%
GAACCAAAGAAACAACTCAA+GGG - chr7_1:46003772-46003791 Msa1021570:exon 35.0%
!! ATACCCTTGAGTTGTTTCTT+TGG + chr7_1:46003778-46003797 None:intergenic 35.0%
GGCAGCATTGTGAAAAAAAT+TGG + chr7_1:46004469-46004488 None:intergenic 35.0%
AGTCGTATAAGCCAAACAAT+TGG + chr7_1:46004490-46004509 None:intergenic 35.0%
TAAGGGAGAAAAAGAGCAAT+AGG + chr7_1:46005491-46005510 None:intergenic 35.0%
CAATAAGGGAAGGAAACATA+AGG + chr7_1:46005509-46005528 None:intergenic 35.0%
ACATCACAATCAATAAGGGA+AGG + chr7_1:46005519-46005538 None:intergenic 35.0%
! TTCCCTTATTGATTGTGATG+TGG - chr7_1:46005518-46005537 Msa1021570:CDS 35.0%
AGCCACATCACAATCAATAA+GGG + chr7_1:46005523-46005542 None:intergenic 35.0%
TAGCCACATCACAATCAATA+AGG + chr7_1:46005524-46005543 None:intergenic 35.0%
! TTGTTTCTTTGGTTCAGCTG+AGG + chr7_1:46003767-46003786 None:intergenic 40.0%
! TTGGACCTGCACTAAAGTTT+TGG - chr7_1:46004205-46004224 Msa1021570:intron 40.0%
TCACAATGCTGCCAATTGTT+TGG - chr7_1:46004476-46004495 Msa1021570:intron 40.0%
AAAGAAGACCTCTCTAGACA+CGG - chr7_1:46004592-46004611 Msa1021570:intron 40.0%
TAATTCTTCCGTGTCTAGAG+AGG + chr7_1:46004603-46004622 None:intergenic 40.0%
CAATGTTTCCTGCAAGATCT+TGG + chr7_1:46005305-46005324 None:intergenic 40.0%
CAAGATCTTGCAGGAAACAT+TGG - chr7_1:46005303-46005322 Msa1021570:intron 40.0%
TATTAGGAGGCTTCAGAAAG+AGG - chr7_1:46005330-46005349 Msa1021570:intron 40.0%
TGAGAGGCTAACACAATTGA+TGG - chr7_1:46005366-46005385 Msa1021570:intron 40.0%
! TTGTTTCTTTGGTTCAGCTG+AGG + chr7_1:46003767-46003786 None:intergenic 40.0%
! TTGGACCTGCACTAAAGTTT+TGG - chr7_1:46004205-46004224 Msa1021570:intron 40.0%
TCACAATGCTGCCAATTGTT+TGG - chr7_1:46004476-46004495 Msa1021570:intron 40.0%
AAAGAAGACCTCTCTAGACA+CGG - chr7_1:46004592-46004611 Msa1021570:intron 40.0%
TAATTCTTCCGTGTCTAGAG+AGG + chr7_1:46004603-46004622 None:intergenic 40.0%
CAATGTTTCCTGCAAGATCT+TGG + chr7_1:46005305-46005324 None:intergenic 40.0%
CAAGATCTTGCAGGAAACAT+TGG - chr7_1:46005303-46005322 Msa1021570:intron 40.0%
TATTAGGAGGCTTCAGAAAG+AGG - chr7_1:46005330-46005349 Msa1021570:intron 40.0%
TGAGAGGCTAACACAATTGA+TGG - chr7_1:46005366-46005385 Msa1021570:intron 40.0%
CCAAGCCAAAACTTTAGTGC+AGG + chr7_1:46004213-46004232 None:intergenic 45.0%
!!! CCTGCACTAAAGTTTTGGCT+TGG - chr7_1:46004210-46004229 Msa1021570:intron 45.0%
GTGTCTAGAGAGGTCTTCTT+TGG + chr7_1:46004593-46004612 None:intergenic 45.0%
TGTTTCCTGCAAGATCTTGG+AGG + chr7_1:46005302-46005321 None:intergenic 45.0%
CCAAGCCAAAACTTTAGTGC+AGG + chr7_1:46004213-46004232 None:intergenic 45.0%
!!! CCTGCACTAAAGTTTTGGCT+TGG - chr7_1:46004210-46004229 Msa1021570:intron 45.0%
GTGTCTAGAGAGGTCTTCTT+TGG + chr7_1:46004593-46004612 None:intergenic 45.0%
TGTTTCCTGCAAGATCTTGG+AGG + chr7_1:46005302-46005321 None:intergenic 45.0%
!!! TATTTTTAAAAAAAATACTT+AGG - chr7_1:46003982-46004001 Msa1021570:intron 5.0%
!!! TATTTTTAAAAAAAATACTT+AGG - chr7_1:46003982-46004001 Msa1021570:intron 5.0%
ATCAGCCTCCAAGATCTTGC+AGG - chr7_1:46005294-46005313 Msa1021570:intron 50.0%
TAGGAGGCTTCAGAAAGAGG+TGG - chr7_1:46005333-46005352 Msa1021570:intron 50.0%
AGGTGGAAGATCTCAGTGAG+AGG - chr7_1:46005350-46005369 Msa1021570:intron 50.0%
ATCAGCCTCCAAGATCTTGC+AGG - chr7_1:46005294-46005313 Msa1021570:intron 50.0%
TAGGAGGCTTCAGAAAGAGG+TGG - chr7_1:46005333-46005352 Msa1021570:intron 50.0%
AGGTGGAAGATCTCAGTGAG+AGG - chr7_1:46005350-46005369 Msa1021570:intron 50.0%
Chromosome Type Strat End Strand Name
chr7_1 gene 46003625 46005609 46003625 ID=Msa1021570;Name=Msa1021570
chr7_1 mRNA 46003625 46005609 46003625 ID=Msa1021570-mRNA-1;Parent=Msa1021570;Name=Msa1021570-mRNA-1;_AED=0.00;_eAED=0.00;_QI=55|1|1|1|1|1|2|162|89
chr7_1 exon 46003625 46003942 46003625 ID=Msa1021570-mRNA-1:exon:9138;Parent=Msa1021570-mRNA-1
chr7_1 exon 46005441 46005609 46005441 ID=Msa1021570-mRNA-1:exon:9137;Parent=Msa1021570-mRNA-1
chr7_1 five_prime_UTR 46005555 46005609 46005555 ID=Msa1021570-mRNA-1:five_prime_utr;Parent=Msa1021570-mRNA-1
chr7_1 CDS 46005441 46005554 46005441 ID=Msa1021570-mRNA-1:cds;Parent=Msa1021570-mRNA-1
chr7_1 CDS 46003787 46003942 46003787 ID=Msa1021570-mRNA-1:cds;Parent=Msa1021570-mRNA-1
chr7_1 three_prime_UTR 46003625 46003786 46003625 ID=Msa1021570-mRNA-1:three_prime_utr;Parent=Msa1021570-mRNA-1
Gene Sequence

>Msa1021570

ATGTCTGGCCAGAGAAACTCAAGAGATTCAAAGTTGAAAGAGAAACAGATTAATGATCTTGTGTCAAGGTTACAACTACTATTGCCTCAGCTGAACCAAAGAAACAACTCAAGGCAATCAGCCTCCAAGATCTTGCAGGAAACATTGGATCATATTAGGAGGCTTCAGAAAGAGGTGGAAGATCTCAGTGAGAGGCTAACACAATTGATGGATTCTGCAGATATCAATGATAATGACAGAAGAACTTTAGAGAACTTTTTTCAATATTAA

Protein sequence

>Msa1021570

MSGQRNSRDSKLKEKQINDLVSRLQLLLPQLNQRNNSRQSASKILQETLDHIRRLQKEVEDLSERLTQLMDSADINDNDRRTLENFFQY*