AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1120470

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1120470 MtrunA17_Chr7g0251591 97.849 744 16 0 1 744 1 744 0.0 1484
Msa1120470 MtrunA17_Chr3g0131841 49.383 162 74 3 349 509 293 447 1.88e-35 140
Msa1120470 MtrunA17_Chr3g0131831 52.525 99 37 2 647 743 91 181 1.23e-25 104
Msa1120470 MtrunA17_Chr3g0131831 70.909 55 16 0 508 562 12 66 4.28e-17 80.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1120470 AT3G26890.5 41.457 755 322 23 1 743 1 647 1.19e-146 444
Msa1120470 AT3G26890.1 41.457 755 322 23 1 743 1 647 1.19e-146 444
Msa1120470 AT3G26890.4 41.457 755 322 23 1 743 1 647 1.19e-146 444
Msa1120470 AT3G26890.3 41.457 755 322 23 1 743 1 647 1.19e-146 444
Msa1120470 AT3G26890.7 41.457 755 322 23 1 743 1 647 1.19e-146 444
Msa1120470 AT3G26890.8 41.457 755 322 23 1 743 1 647 1.19e-146 444
Msa1120470 AT3G26890.2 41.457 755 322 23 1 743 1 647 1.19e-146 444
Msa1120470 AT3G26890.6 41.457 755 321 24 1 743 1 646 7.13e-145 439
Msa1120470 AT5G41110.1 43.199 669 226 24 93 743 89 621 7.97e-134 409
Msa1120470 AT5G41110.2 43.199 669 226 24 93 743 89 621 7.97e-134 409
Msa1120470 AT5G41110.3 42.657 429 179 16 93 509 89 462 1.25e-71 241

Find 220 sgRNAs with CRISPR-Local

Find 42 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTATCTGGCCGGTTTGTTTC+TGG 0.108815 7_3:-69863566 Msa1120470:CDS
CTACTTGTGTAATGATTTAA+AGG 0.129935 7_3:-69860815 Msa1120470:CDS
CTTGCCGTGTTAAGTATTTC+TGG 0.145625 7_3:-69863384 Msa1120470:CDS
CAACAATGTCAATAATTTAA+TGG 0.166881 7_3:-69859001 Msa1120470:three_prime_UTR
AATGGAAGCTTCTGTGATTT+AGG 0.225625 7_3:+69863348 None:intergenic
GGGTTCTTACTCCTGCTTCC+AGG 0.259187 7_3:-69864480 Msa1120470:CDS
TTTGTTCCAGTTGCAGTTCT+TGG 0.264094 7_3:-69865206 Msa1120470:five_prime_UTR
TCGAAGTATCTTGGATCTTC+AGG 0.267708 7_3:+69859171 None:intergenic
GCAGTTATACAATGCCTTCT+TGG 0.268730 7_3:-69864159 Msa1120470:CDS
TTCTTCCTTTCATTGGTATA+CGG 0.280459 7_3:+69863178 None:intergenic
CATTTCTAATGAGGCATTCT+TGG 0.283943 7_3:+69864621 None:intergenic
TGATGAAAATGTCGAACTTT+TGG 0.288161 7_3:-69864767 Msa1120470:CDS
GCAAATCTCCATTCTTCTTT+TGG 0.289993 7_3:-69864394 Msa1120470:CDS
ATCATCCACTATGGATTGTT+TGG 0.290000 7_3:-69865262 Msa1120470:five_prime_UTR
TCGACACTAGTAACAGAAAA+TGG 0.292636 7_3:+69863330 None:intergenic
GACATTGTTGAAGTATTTAC+AGG 0.295023 7_3:+69859014 None:intergenic
AACAATGTCAATAATTTAAT+GGG 0.296411 7_3:-69859000 Msa1120470:three_prime_UTR
GATAATAAGAAGGCAAATAT+TGG 0.299727 7_3:-69864190 Msa1120470:CDS
TGTATATAGTTACATGCTAT+TGG 0.317266 7_3:-69859086 Msa1120470:three_prime_UTR
CTATTAAAATTGAACACTTA+AGG 0.322313 7_3:-69864021 Msa1120470:CDS
AAGAACCCTCCGTTCAGTTT+TGG 0.325248 7_3:+69864495 None:intergenic
AGAATTTCCCGCAAGATCTA+TGG 0.325534 7_3:+69863278 None:intergenic
TTCCAATTGTAGTTGTTAAT+AGG 0.329949 7_3:-69865056 Msa1120470:five_prime_UTR
AGCTGAATTCTTCCTTTCAT+TGG 0.332758 7_3:+69863171 None:intergenic
CGGGAAATTCTTTATCAAAC+AGG 0.333370 7_3:-69863266 Msa1120470:CDS
ATTTCCTGAAAGAATGAAAA+TGG 0.335182 7_3:-69863909 Msa1120470:CDS
GGCTTCATCTGCTGCGAGTA+TGG 0.338263 7_3:-69865240 Msa1120470:five_prime_UTR
CAGTTGATGTCGAAGTATCT+TGG 0.338633 7_3:+69859162 None:intergenic
ACTTTCAGTCAGAAGGTCTT+TGG 0.340125 7_3:-69863612 Msa1120470:CDS
CATAGATACAAACCTTAGTA+TGG 0.350741 7_3:+69863540 None:intergenic
ACATAGTATGAAGTTCTTGT+AGG 0.352298 7_3:-69865108 Msa1120470:five_prime_UTR
AGAAGATGAGATAAGGGTTA+AGG 0.354226 7_3:+69866175 Msa1120480:CDS
CCCTTCTGCAATTCAACCTT+GGG 0.355021 7_3:+69866033 Msa1120480:CDS
TATTAAAATTGAACACTTAA+GGG 0.358392 7_3:-69864020 Msa1120470:CDS
TCATCCACTATGGATTGTTT+GGG 0.364717 7_3:-69865261 Msa1120470:five_prime_UTR
ATTTCTAATGAGGCATTCTT+GGG 0.368123 7_3:+69864622 None:intergenic
TTGCCGTGTTAAGTATTTCT+GGG 0.370614 7_3:-69863383 Msa1120470:CDS
TTCATTCTTTCAGGAAATCT+AGG 0.372002 7_3:+69863914 None:intergenic
GAGAAAACATGTGATGAATC+AGG 0.378217 7_3:-69861012 Msa1120470:CDS
GTCTTTGGTTGGTTCATTTG+AGG 0.384587 7_3:-69863597 Msa1120470:CDS
GTATGAAGTTCTTGTAGGTT+TGG 0.385354 7_3:-69865103 Msa1120470:five_prime_UTR
CTGCTCAAAGCAATTTGATA+AGG 0.387117 7_3:+69864135 None:intergenic
AGATTTCTGTCCATCTTCTC+TGG 0.387579 7_3:-69863720 Msa1120470:CDS
GGAATGAAAGAAAGGAGTCT+TGG 0.390571 7_3:-69861035 Msa1120470:CDS
AATTCTTTATCAAACAGGTT+TGG 0.400272 7_3:-69863261 Msa1120470:CDS
AAAAGATCAAATTAAACAAA+AGG 0.402774 7_3:+69866093 Msa1120480:CDS
GAACCTTGCAGAATTAGAAA+TGG 0.404122 7_3:+69863652 None:intergenic
AATCTTACTTTGGTACCAAT+TGG 0.407975 7_3:+69862557 None:intergenic
ATTAAGTTCTCTTCCAGAAA+AGG 0.409105 7_3:-69860869 Msa1120470:CDS
GAAGATGAGATAAGGGTTAA+GGG 0.410652 7_3:+69866176 Msa1120480:CDS
TAGTTGTTAGCTGATGTTGT+AGG 0.410682 7_3:-69864985 Msa1120470:five_prime_UTR
ACTGACACGCTTACTAGTTT+CGG 0.413584 7_3:+69865014 None:intergenic
AATGTCTTATCAGAAACATT+TGG 0.418483 7_3:+69864598 None:intergenic
TCTTCCAGAAAAGGCTGGAT+TGG 0.420395 7_3:-69860860 Msa1120470:CDS
TACCTATTAACAACTACAAT+TGG 0.423061 7_3:+69865054 None:intergenic
GAATGAAAGAAAGGAGTCTT+GGG 0.423283 7_3:-69861034 Msa1120470:CDS
GCTTCACTTGATGGTATTTG+TGG 0.424333 7_3:+69864790 None:intergenic
TCAGTTAAACTTCCATGATC+TGG 0.425257 7_3:+69861069 None:intergenic
ATCTTGGATCTTCAGGGAAA+TGG 0.426868 7_3:+69859178 None:intergenic
ACTCCTGCTTCCAGGGTTGT+CGG 0.426874 7_3:-69864472 Msa1120470:CDS
ACAGATATTACGCCACTTTG+AGG 0.431291 7_3:+69863977 None:intergenic
ATATATCCAAGAACTGCAAC+TGG 0.431562 7_3:+69865200 None:intergenic
GACCAATGAAGTGGCAGATT+CGG 0.433702 7_3:+69864351 None:intergenic
TAGGTTTGGAATCATTTGAT+TGG 0.438295 7_3:-69865089 Msa1120470:five_prime_UTR
ACTGTTAGAACTCTTTGAAC+AGG 0.439854 7_3:+69860975 None:intergenic
AACATTTGGCATTTCTAATG+AGG 0.440023 7_3:+69864612 None:intergenic
GTCATTTCCACTGTCCAAAC+CGG 0.441003 7_3:+69861260 None:intergenic
AAGATGAAGAAGATGAGATA+AGG 0.442638 7_3:+69866168 Msa1120480:CDS
AAACACAAAGGTTTCTAGCT+TGG 0.446488 7_3:-69861130 Msa1120470:CDS
CCTCTTTCGTTGTCACCCCT+CGG 0.447832 7_3:-69863938 Msa1120470:CDS
CTTCTGTTTCCACCTTTCAT+CGG 0.452187 7_3:-69865983 Msa1120470:intron
CACCGAATCTGCCACTTCAT+TGG 0.454316 7_3:-69864353 Msa1120470:CDS
TAAGCCCTCGTTTCAGAAGT+TGG 0.456282 7_3:+69863235 None:intergenic
TAGGTCTTACCGTGATCATC+TGG 0.457601 7_3:-69864966 Msa1120470:five_prime_UTR
GGTTCTTACTCCTGCTTCCA+GGG 0.461629 7_3:-69864479 Msa1120470:CDS
TCCATGATCTGGTGAGTGCT+TGG 0.461711 7_3:+69861080 None:intergenic
AGTTCTCTTCCAGAAAAGGC+TGG 0.462804 7_3:-69860865 Msa1120470:CDS
CATAGTGGATGATCAGTTCA+TGG 0.463238 7_3:+69865272 None:intergenic
CTTTCATTGGTATACGGAAA+CGG 0.463415 7_3:+69863184 None:intergenic
GGTCCTTTGCTTGAAAATAG+AGG 0.463606 7_3:-69864070 Msa1120470:CDS
GTGACTTAAGTATTAGAGAT+GGG 0.470105 7_3:-69864817 Msa1120470:exon
ACCCTTCTGCAATTCAACCT+TGG 0.471155 7_3:+69866032 Msa1120480:CDS
TAATAGTTGGTGAACTACCC+TGG 0.473332 7_3:+69864038 None:intergenic
AGTGATGTTGAAATGCTTTG+TGG 0.479179 7_3:-69858932 Msa1120470:three_prime_UTR
CATGAACTGATCATCCACTA+TGG 0.479524 7_3:-69865271 Msa1120470:five_prime_UTR
AAGGTGGAAACAGAAGAATC+AGG 0.480188 7_3:+69865990 Msa1120480:CDS
CGAAGTATCTTGGATCTTCA+GGG 0.483518 7_3:+69859172 None:intergenic
TCCAAGCACTCACCAGATCA+TGG 0.487462 7_3:-69861081 Msa1120470:CDS
CGCCTAGAAAATAATTCAAA+GGG 0.489033 7_3:+69858894 None:intergenic
TATGCATCCATAGATCTTGC+GGG 0.489705 7_3:-69863285 Msa1120470:CDS
TGAAAATGGCCACAGGATGC+AGG 0.490719 7_3:-69863895 Msa1120470:CDS
GAAATGTCGTCCAGAGAAGA+TGG 0.495083 7_3:+69863710 None:intergenic
AGATGAAGAAGATGAGATAA+GGG 0.495996 7_3:+69866169 Msa1120480:CDS
TGCATTACTTCCCCACTACA+CGG 0.497521 7_3:+69861201 None:intergenic
CAAATTAAACAAAAGGGTGT+TGG 0.501179 7_3:+69866100 Msa1120480:intron
TCTTTATCAAACAGGTTTGG+AGG 0.501364 7_3:-69863258 Msa1120470:CDS
TCGTTAATTATTTGATCCCA+AGG 0.502467 7_3:-69866049 Msa1120470:five_prime_UTR
AAAGGCGATTCTCTTGACAT+AGG 0.502608 7_3:-69864277 Msa1120470:CDS
TTCATGCTGCTTATAGAAGA+GGG 0.503762 7_3:+69864703 None:intergenic
TAGAAAAGATGATTCACAAA+TGG 0.504941 7_3:-69863214 Msa1120470:CDS
TGTTTCTGGAAACCATACTA+AGG 0.505232 7_3:-69863552 Msa1120470:intron
GAAGAATGGAGATTTGCTAC+TGG 0.505944 7_3:+69864400 None:intergenic
AATAGTTGGTGAACTACCCT+GGG 0.507763 7_3:+69864039 None:intergenic
ATGTGAGAGGAATGAAAGAA+AGG 0.508447 7_3:-69861043 Msa1120470:CDS
ACAGATGTTAATGTTGTTGT+GGG 0.508480 7_3:-69866244 Msa1120470:five_prime_UTR
TATGGTTTCCAGAAACAAAC+CGG 0.508917 7_3:+69863558 None:intergenic
AGATACTACCAAAAGAAGAA+TGG 0.509004 7_3:+69864386 None:intergenic
TAATGCACATCTTACAATGC+TGG 0.509050 7_3:+69858868 None:intergenic
ATGAATAGTGTAAACACTCA+TGG 0.509594 7_3:-69864685 Msa1120470:CDS
AAAGATCAAATTAAACAAAA+GGG 0.510854 7_3:+69866094 Msa1120480:CDS
TGACTGAGGGGAAGTTGAGC+TGG 0.511860 7_3:+69861296 None:intergenic
AATCTGCCACTTCATTGGTC+AGG 0.513369 7_3:-69864348 Msa1120470:CDS
GAAGTATTTACAGGAGGAAG+AGG 0.515600 7_3:+69859023 None:intergenic
AGGACAATGGCCTCAAGTAA+AGG 0.516150 7_3:+69859063 None:intergenic
AGTGACTTAAGTATTAGAGA+TGG 0.518035 7_3:-69864818 Msa1120470:exon
AACCTTGCAGAATTAGAAAT+GGG 0.518979 7_3:+69863653 None:intergenic
GGGTGTGCTGCATGTACCCT+TGG 0.519395 7_3:-69864664 Msa1120470:CDS
TCAGTCAGAAGGTCTTTGGT+TGG 0.520387 7_3:-69863608 Msa1120470:CDS
AGATGGACAGAAATCTTTGA+AGG 0.523799 7_3:+69863727 None:intergenic
TTTCAGGAAATCTAGGACCG+AGG 0.525504 7_3:+69863921 None:intergenic
CCGCCTAGAAAATAATTCAA+AGG 0.526092 7_3:+69858893 None:intergenic
TGCCGTGTTAAGTATTTCTG+GGG 0.526820 7_3:-69863382 Msa1120470:CDS
GACAGATGTTAATGTTGTTG+TGG 0.529667 7_3:-69866245 Msa1120470:five_prime_UTR
AGAACAAGTTCTTTAACTGA+TGG 0.530111 7_3:-69864436 Msa1120470:CDS
TTCAGATGCCGACGAGGGAA+AGG 0.531155 7_3:-69860773 Msa1120470:intron
AGTGGCAGATTCGGTGTCAT+TGG 0.531398 7_3:+69864360 None:intergenic
CCGAGGGGTGACAACGAAAG+AGG 0.532056 7_3:+69863938 None:intergenic
TGAGTGGACTTTCAGTCAGA+AGG 0.536067 7_3:-69863619 Msa1120470:CDS
ATCATGACAACCCGTGTAGT+GGG 0.536673 7_3:-69861212 Msa1120470:CDS
GATCTTCAAACTGTGGACAT+TGG 0.537193 7_3:+69864549 None:intergenic
TTCAGGAAATCTAGGACCGA+GGG 0.537725 7_3:+69863922 None:intergenic
ATGCTATTGGCCTTTACTTG+AGG 0.538301 7_3:-69859073 Msa1120470:three_prime_UTR
GGCTGGATTGGACATGGAAG+GGG 0.539426 7_3:-69860848 Msa1120470:CDS
AGGCTGGATTGGACATGGAA+GGG 0.543736 7_3:-69860849 Msa1120470:CDS
TGCTTCCAAAACTGAACGGA+GGG 0.546214 7_3:-69864500 Msa1120470:CDS
AAATAGAGGCTTCATATCCC+AGG 0.546921 7_3:-69864056 Msa1120470:CDS
CTTCTTATTATCTTGTGCAG+TGG 0.547056 7_3:+69864201 None:intergenic
AACATTATCATTCTTAACAA+GGG 0.548514 7_3:+69864231 None:intergenic
TCAAAGAGTTCTAACAGTGC+TGG 0.548643 7_3:-69860970 Msa1120470:CDS
AAACCGACAACCCTGGAAGC+AGG 0.550114 7_3:+69864469 None:intergenic
CTATGTTATGTTTATATCAG+AGG 0.550580 7_3:+69865125 None:intergenic
GATTTGAGTGACATGCCAAT+TGG 0.552113 7_3:-69862572 Msa1120470:CDS
AATTACAACATCAACTGCAT+CGG 0.553363 7_3:+69861176 None:intergenic
TTGCAAAAGAATGTGTGAAG+AGG 0.553725 7_3:+69862599 None:intergenic
CACCCATTTCTAATTCTGCA+AGG 0.554901 7_3:-69863655 Msa1120470:CDS
TTATGCATCCATAGATCTTG+CGG 0.558647 7_3:-69863286 Msa1120470:CDS
GAATCGCCTTTGAAATGACT+CGG 0.559073 7_3:+69864289 None:intergenic
AGTTCAACCGGTTTGGACAG+TGG 0.559482 7_3:-69861267 Msa1120470:CDS
GAGGCCAACTTCTGAAACGA+GGG 0.559815 7_3:-69863239 Msa1120470:CDS
AATCGCCTTTGAAATGACTC+GGG 0.559889 7_3:+69864290 None:intergenic
AATCATGACAACCCGTGTAG+TGG 0.559925 7_3:-69861213 Msa1120470:CDS
AAGGCTGGATTGGACATGGA+AGG 0.560734 7_3:-69860850 Msa1120470:CDS
TCCCAAGGTTGAATTGCAGA+AGG 0.561224 7_3:-69866034 Msa1120470:five_prime_UTR
AATGAAAGGAAGAATTCAGC+TGG 0.561386 7_3:-69863169 Msa1120470:intron
CATAGGATGCAGAAATAATG+AGG 0.570164 7_3:-69864260 Msa1120470:CDS
AGAAAAGGCTGGATTGGACA+TGG 0.570456 7_3:-69860854 Msa1120470:CDS
TACCGTGATCATCTGGTGAA+AGG 0.570721 7_3:-69864959 Msa1120470:five_prime_UTR
TGAATAGTGTAAACACTCAT+GGG 0.577406 7_3:-69864684 Msa1120470:CDS
TAACATTATCATTCTTAACA+AGG 0.577731 7_3:+69864230 None:intergenic
GATTGGACATGGAAGGGGAA+CGG 0.579510 7_3:-69860843 Msa1120470:CDS
AGGTGGAAACAGAAGAATCA+GGG 0.580400 7_3:+69865991 Msa1120480:CDS
TGATTCAAAACCGACAACCC+TGG 0.581707 7_3:+69864462 None:intergenic
TGGACAGTGGAAATGACAAA+GGG 0.582026 7_3:-69861254 Msa1120470:CDS
CACTGCACAAGATAATAAGA+AGG 0.585137 7_3:-69864200 Msa1120470:CDS
AGAAAACATGTGATGAATCA+GGG 0.586761 7_3:-69861011 Msa1120470:CDS
ATTCATGCTGCTTATAGAAG+AGG 0.587032 7_3:+69864702 None:intergenic
CGTTTCCGTATACCAATGAA+AGG 0.592221 7_3:-69863183 Msa1120470:CDS
TTCCTTTCACCAGATGATCA+CGG 0.593746 7_3:+69864957 None:intergenic
TCGCTTCCTGACCAATGAAG+TGG 0.594131 7_3:+69864342 None:intergenic
CTGCTTCCAAAACTGAACGG+AGG 0.595798 7_3:-69864501 Msa1120470:CDS
TTATTGCAATGTCTCAAACA+TGG 0.596189 7_3:-69865151 Msa1120470:five_prime_UTR
TATGCATACCTTTCCCTCGT+CGG 0.599027 7_3:+69860765 None:intergenic
GAAGCCCAAACAATCCATAG+TGG 0.599274 7_3:+69865257 None:intergenic
GAAAGAATGAAAATGGCCAC+AGG 0.599520 7_3:-69863902 Msa1120470:CDS
GCATTACTTCCCCACTACAC+GGG 0.602906 7_3:+69861202 None:intergenic
GTATATCTGTAGAAAATCGA+TGG 0.605410 7_3:-69863411 Msa1120470:intron
CCCAAGGTTGAATTGCAGAA+GGG 0.607886 7_3:-69866033 Msa1120470:five_prime_UTR
GCAAGTGGGTGTGTGTGCGG+TGG 0.609211 7_3:-69866326 None:intergenic
AGGTTCACTAGAAGTTTGAG+TGG 0.609481 7_3:-69863635 Msa1120470:CDS
CAAGTGGGTGTGTGTGCGGT+GGG 0.610344 7_3:-69866325 None:intergenic
TTGAATTGATCTTCAAACTG+TGG 0.610582 7_3:+69864542 None:intergenic
TGGGTTGTTGAAATTGCAGA+AGG 0.610753 7_3:+69866143 Msa1120480:intron
CAATTTGATAAGGACCAAGA+AGG 0.612047 7_3:+69864145 None:intergenic
TTGGACAGTGGAAATGACAA+AGG 0.613787 7_3:-69861255 Msa1120470:CDS
GTCTATTTGGCAGATGAATG+TGG 0.614657 7_3:-69859205 Msa1120470:intron
CTCTCCCCGAGTCATTTCAA+AGG 0.615400 7_3:-69864295 Msa1120470:CDS
AACGAAAGAGGAGAGCTCAC+AGG 0.615548 7_3:+69863950 None:intergenic
TCATGCTGCTTATAGAAGAG+GGG 0.617476 7_3:+69864704 None:intergenic
TTTCTGCTTCCAAAACTGAA+CGG 0.620837 7_3:-69864504 Msa1120470:CDS
AGCATGTAACTATATACAAG+AGG 0.620915 7_3:+69859091 None:intergenic
GTTGACACTCCTGCATCCTG+TGG 0.622325 7_3:+69863886 None:intergenic
AAGGGCAGATAAAGCGAAGG+TGG 0.626406 7_3:+69860929 None:intergenic
GGTGGAGAGCATAACGAAGT+GGG 0.628379 7_3:+69860947 None:intergenic
AAGTATTTACAGGAGGAAGA+GGG 0.628616 7_3:+69859024 None:intergenic
AATAGAGGCTTCATATCCCA+GGG 0.631162 7_3:-69864055 Msa1120470:CDS
ATCAATGTGATCCCTCAGCA+AGG 0.634775 7_3:+69864910 None:intergenic
ATTGTTGAAGTATTTACAGG+AGG 0.636960 7_3:+69859017 None:intergenic
TTTCTAATGAGGCATTCTTG+GGG 0.639052 7_3:+69864623 None:intergenic
GTTTAACTGATGAATGTGAG+AGG 0.640480 7_3:-69861056 Msa1120470:CDS
CGTCATTCAGATGCCGACGA+GGG 0.641258 7_3:-69860778 Msa1120470:CDS
GCGTCATTCAGATGCCGACG+AGG 0.641458 7_3:-69860779 Msa1120470:CDS
AGGTGGAGAGCATAACGAAG+TGG 0.642212 7_3:+69860946 None:intergenic
ATAGCAAGTGGGTGTGTGTG+CGG 0.645627 7_3:-69866329 None:intergenic
AACATGTAAGACCTTGCTGA+GGG 0.646250 7_3:-69864921 Msa1120470:five_prime_UTR
TCATGACAACCCGTGTAGTG+GGG 0.648256 7_3:-69861211 Msa1120470:CDS
GGAGGCCAACTTCTGAAACG+AGG 0.650557 7_3:-69863240 Msa1120470:CDS
GAACATGTAAGACCTTGCTG+AGG 0.656132 7_3:-69864922 Msa1120470:five_prime_UTR
GCCGTGTTAAGTATTTCTGG+GGG 0.663682 7_3:-69863381 Msa1120470:CDS
GCATGTAACTATATACAAGA+GGG 0.664324 7_3:+69859092 None:intergenic
ATCGCCTTTGAAATGACTCG+GGG 0.672542 7_3:+69864291 None:intergenic
TACAAATCAGAGAAACACAA+AGG 0.679254 7_3:-69861142 Msa1120470:CDS
CTTAAGGGAATCAAGTGTTG+TGG 0.681377 7_3:-69864005 Msa1120470:CDS
GAAAAGGGCAGATAAAGCGA+AGG 0.690630 7_3:+69860926 None:intergenic
GGGGAGAGCATACTGCTCAG+TGG 0.691084 7_3:+69864310 None:intergenic
TCGGCATCTGAATGACGCTG+AGG 0.695206 7_3:+69860784 None:intergenic
GGTGTTGATTCTAGAGAACG+TGG 0.702836 7_3:+69863674 None:intergenic
TGTGTGCGGTGGGAATGATG+AGG 0.713411 7_3:-69866315 Msa1120470:five_prime_UTR
GGGTTTGCATCCGAAAACTG+AGG 0.716204 7_3:+69864724 None:intergenic
GTTGTGGTCAGACCTCAAAG+TGG 0.734315 7_3:-69863989 Msa1120470:CDS
TCAGGAAATCTAGGACCGAG+GGG 0.735298 7_3:+69863923 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GTTTTTTTAAACATAAACAT+TGG - chr7_3:69858832-69858851 Msa1120470:three_prime_UTR 15.0%
!! AACAATGTCAATAATTTAAT+GGG - chr7_3:69866099-69866118 Msa1120470:five_prime_UTR 15.0%
!!! TAGATTTTAAAAGAATGTAA+TGG - chr7_3:69866291-69866310 Msa1120470:five_prime_UTR 15.0%
!!! AGTGTATTTTTTAGTATTGT+TGG - chr7_3:69865733-69865752 Msa1120470:intron 20.0%
!! CAACAATGTCAATAATTTAA+TGG - chr7_3:69866098-69866117 Msa1120470:five_prime_UTR 20.0%
!!! TAATGGCAAGATTTTTTAAA+AGG - chr7_3:69866308-69866327 Msa1120470:five_prime_UTR 20.0%
! TCTATATTTGAACAGAATGT+TGG - chr7_3:69865771-69865790 Msa1120470:intron 25.0%
! ATATATTAGTGTGCATTGTT+TGG - chr7_3:69865838-69865857 Msa1120470:intron 25.0%
! TGTATATAGTTACATGCTAT+TGG - chr7_3:69866013-69866032 Msa1120470:five_prime_UTR 25.0%
GCATGTAACTATATACAAGA+GGG + chr7_3:69866010-69866029 Msa1120480:CDS 30.0%
AGCATGTAACTATATACAAG+AGG + chr7_3:69866011-69866030 Msa1120480:CDS 30.0%
! ATTGTTGAAGTATTTACAGG+AGG + chr7_3:69866085-69866104 Msa1120480:CDS 30.0%
! GACATTGTTGAAGTATTTAC+AGG + chr7_3:69866088-69866107 Msa1120480:CDS 30.0%
! CACCCTTTGAATTATTTTCT+AGG - chr7_3:69866203-69866222 Msa1120470:five_prime_UTR 30.0%
CGCCTAGAAAATAATTCAAA+GGG + chr7_3:69866208-69866227 Msa1120480:CDS 30.0%
! CCTTTGAATTATTTTCTAGG+CGG - chr7_3:69866206-69866225 Msa1120470:five_prime_UTR 30.0%
AACAGAATGTTGGACATGAT+AGG - chr7_3:69865781-69865800 Msa1120470:intron 35.0%
!!! AGGTTTTGTTGTGGTCTATT+TGG - chr7_3:69865881-69865900 Msa1120470:intron 35.0%
!! AAGCATCATGCTTTTGAAGA+GGG + chr7_3:69865980-69865999 None:intergenic 35.0%
!! AAAGCATCATGCTTTTGAAG+AGG + chr7_3:69865981-69866000 None:intergenic 35.0%
!!! GTGCTATTTTTCAAGGACAA+TGG + chr7_3:69866052-69866071 Msa1120480:CDS 35.0%
AAGTATTTACAGGAGGAAGA+GGG + chr7_3:69866078-69866097 Msa1120480:CDS 35.0%
AGTGATGTTGAAATGCTTTG+TGG - chr7_3:69866167-69866186 Msa1120470:five_prime_UTR 35.0%
CCGCCTAGAAAATAATTCAA+AGG + chr7_3:69866209-69866228 Msa1120480:CDS 35.0%
TAATGCACATCTTACAATGC+TGG + chr7_3:69866234-69866253 Msa1120480:CDS 35.0%
AAAACCTTGTGAGACACTAC+AGG + chr7_3:69865868-69865887 None:intergenic 40.0%
!!! TGTCTCACAAGGTTTTGTTG+TGG - chr7_3:69865872-69865891 Msa1120470:intron 40.0%
GTCTATTTGGCAGATGAATG+TGG - chr7_3:69865894-69865913 Msa1120470:intron 40.0%
ATCTTGGATCTTCAGGGAAA+TGG + chr7_3:69865924-69865943 None:intergenic 40.0%
! CGAAGTATCTTGGATCTTCA+GGG + chr7_3:69865930-69865949 None:intergenic 40.0%
! TCGAAGTATCTTGGATCTTC+AGG + chr7_3:69865931-69865950 None:intergenic 40.0%
! CAGTTGATGTCGAAGTATCT+TGG + chr7_3:69865940-69865959 None:intergenic 40.0%
!! AGCATCATGCTTTTGAAGAG+GGG + chr7_3:69865979-69865998 None:intergenic 40.0%
!! ATGCTATTGGCCTTTACTTG+AGG - chr7_3:69866026-69866045 Msa1120470:five_prime_UTR 40.0%
!!! AGGGTCAGTGCTATTTTTCA+AGG + chr7_3:69866059-69866078 Msa1120480:CDS 40.0%
! GAAGTATTTACAGGAGGAAG+AGG + chr7_3:69866079-69866098 Msa1120480:CDS 40.0%
!! CATCATGCTTTTGAAGAGGG+GGG + chr7_3:69865977-69865996 None:intergenic 45.0%
!! GCATCATGCTTTTGAAGAGG+GGG + chr7_3:69865978-69865997 None:intergenic 45.0%
AGGACAATGGCCTCAAGTAA+AGG + chr7_3:69866039-69866058 Msa1120480:CDS 45.0%
!!! ATAATTTTAAAAGATTATAT+TGG - chr7_3:69866253-69866272 Msa1120470:five_prime_UTR 5.0%
TCTGCCTGTAGTGTCTCACA+AGG - chr7_3:69865861-69865880 Msa1120470:intron 50.0%
TGTGTGCGGTGGGAATGATG+AGG - chr7_3:69858784-69858803 Msa1120470:three_prime_UTR 55.0%
Chromosome Type Strat End Strand Name
chr7_3 gene 69858778 69866343 69858778 ID=Msa1120470;Name=Msa1120470
chr7_3 mRNA 69858778 69866343 69858778 ID=Msa1120470-mRNA-1;Parent=Msa1120470;Name=Msa1120470-mRNA-1;_AED=0.08;_eAED=0.08;_QI=834|1|1|1|0.8|0.66|6|383|744
chr7_3 exon 69858778 69859214 69858778 ID=Msa1120470-mRNA-1:exon:17756;Parent=Msa1120470-mRNA-1
chr7_3 exon 69860774 69861352 69860774 ID=Msa1120470-mRNA-1:exon:17755;Parent=Msa1120470-mRNA-1
chr7_3 exon 69862565 69862645 69862565 ID=Msa1120470-mRNA-1:exon:17754;Parent=Msa1120470-mRNA-1
chr7_3 exon 69863170 69863421 69863170 ID=Msa1120470-mRNA-1:exon:17753;Parent=Msa1120470-mRNA-1
chr7_3 exon 69863553 69865295 69863553 ID=Msa1120470-mRNA-1:exon:17752;Parent=Msa1120470-mRNA-1
chr7_3 exon 69865984 69866343 69865984 ID=Msa1120470-mRNA-1:exon:17751;Parent=Msa1120470-mRNA-1
chr7_3 five_prime_UTR 69865984 69866343 69865984 ID=Msa1120470-mRNA-1:five_prime_utr;Parent=Msa1120470-mRNA-1
chr7_3 five_prime_UTR 69864822 69865295 69864822 ID=Msa1120470-mRNA-1:five_prime_utr;Parent=Msa1120470-mRNA-1
chr7_3 CDS 69863553 69864821 69863553 ID=Msa1120470-mRNA-1:cds;Parent=Msa1120470-mRNA-1
chr7_3 CDS 69863170 69863421 69863170 ID=Msa1120470-mRNA-1:cds;Parent=Msa1120470-mRNA-1
chr7_3 CDS 69862565 69862645 69862565 ID=Msa1120470-mRNA-1:cds;Parent=Msa1120470-mRNA-1
chr7_3 CDS 69860774 69861352 69860774 ID=Msa1120470-mRNA-1:cds;Parent=Msa1120470-mRNA-1
chr7_3 CDS 69859161 69859214 69859161 ID=Msa1120470-mRNA-1:cds;Parent=Msa1120470-mRNA-1
chr7_3 three_prime_UTR 69858778 69859160 69858778 ID=Msa1120470-mRNA-1:three_prime_utr;Parent=Msa1120470-mRNA-1
Gene Sequence

>Msa1120470

ATGGGTTTGCCACAAATACCATCAAGTGAAGCTGATGAAAATGTCGAACTTTTGGGTGTGGTTTCAAGCGGCTCGCCTCAGTTTTCGGATGCAAACCCCTCTTCTATAAGCAGCATGAATAGTGTAAACACTCATGGGTGTGCTGCATGTACCCTTGGATCCTCATTTGAAAATTTCCCCAAGAATGCCTCATTAGAAATGCCAAATGTTTCTGATAAGACATTTTATGGAGGAGCGATGGAAGTCACTTCCAATGTCCACAGTTTGAAGATCAATTCAACAGATAGAAGTAATCTTTCTGCTTCCAAAACTGAACGGAGGGTTCTTACTCCTGCTTCCAGGGTTGTCGGTTTTGAATCAGGCAGAACAAGTTCTTTAACTGATGGAATCGCTGAAGTTCCAGTAGCAAATCTCCATTCTTCTTTTGGTAGTATCTCAGCCAATGACACCGAATCTGCCACTTCATTGGTCAGGAAGCGACTTTTATCTCCACTGAGCAGTATGCTCTCCCCGAGTCATTTCAAAGGCGATTCTCTTGACATAGGATGCAGAAATAATGAGGCTTTCTCCCTTGTTAAGAATGATAATGTTAGAAAGTCCACTGCACAAGATAATAAGAAGGCAAATATTGGATGTAAAAGCAGTTATACAATGCCTTCTTGGTCCTTATCAAATTGCTTTGAGCAGAAAAACATGGCACAGAGTAGAGAATCCATTTTTCAAACTGATGGTCCTTTGCTTGAAAATAGAGGCTTCATATCCCAGGGTAGTTCACCAACTATTAAAATTGAACACTTAAGGGAATCAAGTGTTGTGGTCAGACCTCAAAGTGGCGTAATATCTGTATCACCTGTGAGCTCTCCTCTTTCGTTGTCACCCCTCGGTCCTAGATTTCCTGAAAGAATGAAAATGGCCACAGGATGCAGGAGTGTCAACGAAGAGATAAAGAATTGCAATATAACTTTAAGCAGTATAGAAGAATCACTAGAGAATTCTAATTCATGTGATATTTTAGGTCATAAAGATGATGATTTAAAATTTTCACGTAAATCTTTTGAAGATGTTGAATTCTCCTTCAAAGATTTCTGTCCATCTTCTCTGGACGACATTTCTTACATGAATTGTCCACGTTCTCTAGAATCAACACCCATTTCTAATTCTGCAAGGTTCACTAGAAGTTTGAGTGGACTTTCAGTCAGAAGGTCTTTGGTTGGTTCATTTGAGGAGTCTCTTTTATCTGGCCGGTTTGTTTCTGGAAACCATACTAAGAAAATCGATGGTTTTCTTGCCGTGTTAAGTATTTCTGGGGGAAACTTCTCTCCTAAATCACAGAAGCTTCCATTTTCTGTTACTAGTGTCGATGGAGATTGCTATCTATTGTATTATGCATCCATAGATCTTGCGGGAAATTCTTTATCAAACAGGTTTGGAGGCCAACTTCTGAAACGAGGGCTTAGAAAAGATGATTCACAAATGGTCAAAAGCCGTTTCCGTATACCAATGAAAGGAAGAATTCAGCTGGTGCTAAGCAATCCAGAAAAGACTCCTCTTCACACATTCTTTTGCAACTATGATTTGAGTGACATGCCAATTGGTACCAAAACATTCTTGCGCCAGAAAATTACTCTCGAGTCTTCCAGCTCAACTTCCCCTCAGTCAAAAAATAGTTCAACCGGTTTGGACAGTGGAAATGACAAAGGGATTCCAACAGTACAAAAGAATCATGACAACCCGTGTAGTGGGGAAGTAATGCACACCGATGCAGTTGATGTTGTAATTAAAACAAAATCTACAAATCAGAGAAACACAAAGGTTTCTAGCTTGGAGAATTTTCTAAACAAAGAGGATTCTTCCAAGCACTCACCAGATCATGGAAGTTTAACTGATGAATGTGAGAGGAATGAAAGAAAGGAGTCTTGGGAGAAAACATGTGATGAATCAGGGAAATCATTAAATTCCTGTTCAAAGAGTTCTAACAGTGCTGGCCCACTTCGTTATGCTCTCCACCTTCGCTTTATCTGCCCTTTTCCTAAAAAGACCAGTAGATCGGCTCAAAAATGCAAATTAAGTTCTCTTCCAGAAAAGGCTGGATTGGACATGGAAGGGGAACGGAGATTCTACTTGTGTAATGATTTAAAGGTTGTGTTTCCTCAGCGTCATTCAGATGCCGACGAGGGAAAGATGAATGTGGAATACCATTTCCCTGAAGATCCAAGATACTTCGACATCAACTGA
Protein sequence

>Msa1120470

MGLPQIPSSEADENVELLGVVSSGSPQFSDANPSSISSMNSVNTHGCAACTLGSSFENFPKNASLEMPNVSDKTFYGGAMEVTSNVHSLKINSTDRSNLSASKTERRVLTPASRVVGFESGRTSSLTDGIAEVPVANLHSSFGSISANDTESATSLVRKRLLSPLSSMLSPSHFKGDSLDIGCRNNEAFSLVKNDNVRKSTAQDNKKANIGCKSSYTMPSWSLSNCFEQKNMAQSRESIFQTDGPLLENRGFISQGSSPTIKIEHLRESSVVVRPQSGVISVSPVSSPLSLSPLGPRFPERMKMATGCRSVNEEIKNCNITLSSIEESLENSNSCDILGHKDDDLKFSRKSFEDVEFSFKDFCPSSLDDISYMNCPRSLESTPISNSARFTRSLSGLSVRRSLVGSFEESLLSGRFVSGNHTKKIDGFLAVLSISGGNFSPKSQKLPFSVTSVDGDCYLLYYASIDLAGNSLSNRFGGQLLKRGLRKDDSQMVKSRFRIPMKGRIQLVLSNPEKTPLHTFFCNYDLSDMPIGTKTFLRQKITLESSSSTSPQSKNSSTGLDSGNDKGIPTVQKNHDNPCSGEVMHTDAVDVVIKTKSTNQRNTKVSSLENFLNKEDSSKHSPDHGSLTDECERNERKESWEKTCDESGKSLNSCSKSSNSAGPLRYALHLRFICPFPKKTSRSAQKCKLSSLPEKAGLDMEGERRFYLCNDLKVVFPQRHSDADEGKMNVEYHFPEDPRYFDIN*