AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1128630

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1128630 MtrunA17_Chr7g0272861 91.165 249 20 2 1 248 1 248 3.53e-162 457
Msa1128630 MtrunA17_Chr1g0191861 74.603 63 16 0 28 90 9 71 1.27e-27 101
Msa1128630 MtrunA17_Chr1g0191871 68.182 66 20 1 102 166 1 66 3.60e-20 82.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1128630 AT3G05330.1 51.128 266 107 9 1 248 1 261 1.21e-81 252

Find 93 sgRNAs with CRISPR-Local

Find 175 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACAATATGTTCTTAAATTT+TGG 0.039887 7_3:+80547174 None:intergenic
TTTGACCATATTAATATATT+TGG 0.147711 7_3:-80547037 Msa1128630:three_prime_UTR
TTTCCCAAACCAGTTAATTT+AGG 0.199173 7_3:-80548855 Msa1128630:CDS
AAAACAAGTATTTAGCAAAT+AGG 0.240085 7_3:-80548104 Msa1128630:CDS
TGTGTTGTTGCTACTCTATT+TGG 0.253333 7_3:+80547683 None:intergenic
GGTGGAACAAAGCTGGTTTC+TGG 0.263887 7_3:-80549281 Msa1128630:CDS
GTTGTTGATGTTACTCTATT+TGG 0.265736 7_3:+80547896 None:intergenic
ACCTCCTAAATTAACTGGTT+TGG 0.288483 7_3:+80548851 None:intergenic
AGAGACCTTGAAGTTGATTC+TGG 0.292172 7_3:+80548167 None:intergenic
CCTCCTAAATTAACTGGTTT+GGG 0.296949 7_3:+80548852 None:intergenic
TTTGATGCAGCCAATCTCTT+TGG 0.305480 7_3:+80547336 None:intergenic
ACCTTTGTCTCCTTCTAGAT+TGG 0.315687 7_3:-80547474 Msa1128630:three_prime_UTR
GAAGAAGTTGAAGAGAATAA+AGG 0.317119 7_3:+80548030 None:intergenic
CAACAACGCCACACAAGTTT+AGG 0.321234 7_3:-80547952 Msa1128630:CDS
GATGCAAGCAAGAATCTATT+AGG 0.332220 7_3:-80549208 Msa1128630:intron
TTCATTGTATTTATTTCATA+AGG 0.339782 7_3:-80547139 Msa1128630:three_prime_UTR
AAAACATACTCAGAGCTAAA+AGG 0.339987 7_3:-80548257 Msa1128630:CDS
ACACTTCTCTTGCTCTTTGT+TGG 0.348806 7_3:+80547446 None:intergenic
ACCGTACCTCCTAAATTAAC+TGG 0.361045 7_3:+80548846 None:intergenic
TTTGGAGAGTTCAATCTTGT+AGG 0.363209 7_3:+80547527 None:intergenic
AAACAAGTATTTAGCAAATA+GGG 0.363248 7_3:-80548103 Msa1128630:CDS
GTTCAATCTTGTAGGTCTCT+TGG 0.368805 7_3:+80547535 None:intergenic
ACGTTGTCTAGGTGGGGTTT+TGG 0.369866 7_3:+80549488 None:intergenic
GATGCAGCACGATATCTCTT+TGG 0.376546 7_3:+80547509 None:intergenic
AAAGTTGATGGCTTTGTTAG+TGG 0.379685 7_3:-80547237 Msa1128630:three_prime_UTR
CAACAACACCACACAAGTTT+CGG 0.386498 7_3:-80547880 Msa1128630:three_prime_UTR
GGTGTTGTTGATGTTACTTT+TGG 0.392704 7_3:+80547614 None:intergenic
ACTTGTTCTGAGATAAGTTC+TGG 0.395882 7_3:+80549235 None:intergenic
TCATAAACTTATGCGGTGAT+TGG 0.398357 7_3:+80547645 None:intergenic
ACATATTTCTTGCTCTTCAA+TGG 0.405712 7_3:+80547264 None:intergenic
TTCAATCTTGTAGGTCTCTT+GGG 0.406930 7_3:+80547536 None:intergenic
CGGTGATTGGATCCGAAACT+TGG 0.411229 7_3:+80547658 None:intergenic
AAATTCCAAATATATTAATA+TGG 0.414532 7_3:+80547032 None:intergenic
GTTTCACCTACAAGCATTGC+AGG 0.415594 7_3:+80548405 None:intergenic
AAGAAGTTGAAGAGAATAAA+GGG 0.417357 7_3:+80548031 None:intergenic
GTTGGTGCAGCCAATCTAGA+AGG 0.417865 7_3:+80547464 None:intergenic
AGTGTTTGTCTGATAAGAAC+AGG 0.423320 7_3:+80549444 None:intergenic
TCAGAAACTTATGTGGTGAT+TGG 0.427597 7_3:+80547855 None:intergenic
CTAGCCCATGGCCTATTCCT+AGG 0.437592 7_3:+80548075 None:intergenic
ATCTTTCTCTCCTTCGAGAC+TGG 0.438651 7_3:-80547304 Msa1128630:three_prime_UTR
ATAGGGTATCTCCTAGGAAT+AGG 0.439133 7_3:-80548086 Msa1128630:CDS
CAACAACACAACCCAAGTTT+CGG 0.439758 7_3:-80547670 Msa1128630:three_prime_UTR
TCATTGCCTGCAATGCTTGT+AGG 0.449054 7_3:-80548411 Msa1128630:CDS
GCGTTCCAGAATCAACTTCA+AGG 0.450480 7_3:-80548172 Msa1128630:CDS
GATGGATTAGTGTGTTGAAT+AGG 0.466499 7_3:-80547413 Msa1128630:three_prime_UTR
AGAGAAATAGTTTCTTCAGT+TGG 0.467736 7_3:-80548348 Msa1128630:CDS
CAAGAGTTCTCCAAAGAGAT+TGG 0.474726 7_3:-80547346 Msa1128630:three_prime_UTR
GAGCAAGAAAAGTGATGGAT+TGG 0.487892 7_3:-80547379 Msa1128630:three_prime_UTR
AGTTGAAGAGAATAAAGGGT+TGG 0.502494 7_3:+80548035 None:intergenic
TGCTCTATTTCTTGATATGA+CGG 0.502983 7_3:+80547976 None:intergenic
GTTGAAGAGAATAAAGGGTT+GGG 0.506043 7_3:+80548036 None:intergenic
CCCAAACCAGTTAATTTAGG+AGG 0.507986 7_3:-80548852 Msa1128630:intron
GAACTACAATTCACTGTGGC+AGG 0.508994 7_3:-80549302 Msa1128630:CDS
ACATATTACGTTGTCTAGGT+GGG 0.516327 7_3:+80549481 None:intergenic
GGCAATTGTACTGTTGTAGC+TGG 0.517437 7_3:+80547201 None:intergenic
CATATTACGTTGTCTAGGTG+GGG 0.533124 7_3:+80549482 None:intergenic
CTCCTTCGAGACTGGTAACA+AGG 0.534952 7_3:-80547296 Msa1128630:three_prime_UTR
CTGGAACGCACTTGTTGAGA+TGG 0.535724 7_3:+80548186 None:intergenic
CCAAAGAGCAAGAAAAGTGA+TGG 0.536346 7_3:-80547384 Msa1128630:three_prime_UTR
GTTTCGATCAGAAACTTATG+AGG 0.538941 7_3:+80547920 None:intergenic
TTTGAAGGTGGATCAGTGCA+TGG 0.541390 7_3:-80549336 Msa1128630:intron
ACTCAGAGCTAAAAGGAGAA+AGG 0.547146 7_3:-80548250 Msa1128630:CDS
TGTGGCAGGTGGAACAAAGC+TGG 0.548780 7_3:-80549288 Msa1128630:CDS
TATCTCCTAGGAATAGGCCA+TGG 0.549739 7_3:-80548080 Msa1128630:CDS
CTTGTAGGTCTCTTGGGTGA+AGG 0.550040 7_3:+80547542 None:intergenic
GCTAACATATTACGTTGTCT+AGG 0.559526 7_3:+80549477 None:intergenic
AGAAGTGTACAGAAAAGTGA+TGG 0.573647 7_3:-80547431 Msa1128630:three_prime_UTR
AACATATTACGTTGTCTAGG+TGG 0.573679 7_3:+80549480 None:intergenic
TAGCAAATAGGGTATCTCCT+AGG 0.574329 7_3:-80548092 Msa1128630:CDS
GTAGGTCTCTTGGGTGAAGG+TGG 0.578281 7_3:+80547545 None:intergenic
AGTACTGTCTTTCTAGCCCA+TGG 0.578757 7_3:+80548063 None:intergenic
ATCTCCTAGGAATAGGCCAT+GGG 0.582839 7_3:-80548079 Msa1128630:CDS
CAACAACACCACAAAAGTCT+CGG 0.586382 7_3:-80547601 Msa1128630:three_prime_UTR
AGCCTTGTTACCAGTCTCGA+AGG 0.600893 7_3:+80547294 None:intergenic
CGCACTTGTTGAGATGGTAA+TGG 0.601173 7_3:+80548192 None:intergenic
GATTTGATCAGAAACTTATG+TGG 0.606522 7_3:+80547569 None:intergenic
CAGAGCTAAAAGGAGAAAGG+AGG 0.616146 7_3:-80548247 Msa1128630:CDS
CTACAATTCACTGTGGCAGG+TGG 0.624153 7_3:-80549299 Msa1128630:CDS
AGGTGGATCAGTGCATGGAT+CGG 0.626730 7_3:-80549331 Msa1128630:CDS
GGTGATTGGATCCGAAACTT+GGG 0.626899 7_3:+80547659 None:intergenic
GATTGGATCCGAAACTTGTG+TGG 0.641283 7_3:+80547872 None:intergenic
GATTTGATCCTAAACTTGTG+TGG 0.652691 7_3:+80547944 None:intergenic
GCCAATCTAGAAGGAGACAA+AGG 0.660648 7_3:+80547473 None:intergenic
CACATAGTAAGTAGTAACCA+TGG 0.662199 7_3:-80549514 Msa1128630:exon
TATCAGACAAACACTCAACA+AGG 0.669769 7_3:-80549436 Msa1128630:intron
GTAACTTTGATGCAGCTGAG+TGG 0.671723 7_3:-80548443 Msa1128630:intron
GATTTGGTCATAAACTTATG+CGG 0.690111 7_3:+80547638 None:intergenic
AGATAAGTTCTGGTGCTACG+AGG 0.714981 7_3:+80549245 None:intergenic
ACTTGTTGAGATGGTAATGG+TGG 0.716854 7_3:+80548195 None:intergenic
TGGTACAAAATTTGCCACCG+AGG 0.726257 7_3:-80548328 Msa1128630:CDS
GGTTTGGGAAACTTCCCCAG+CGG 0.746127 7_3:+80548867 None:intergenic
CTTACTATGTGTGATCCACG+CGG 0.776784 7_3:+80549526 None:intergenic
ACAAGAACTACAATTCACTG+TGG 0.807781 7_3:-80549306 Msa1128630:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTAAAATAAAATGGATTAA+GGG + chr7_3:80547178-80547197 None:intergenic 10.0%
!!! AATTAAAATAAAATGGATTA+AGG + chr7_3:80547179-80547198 None:intergenic 10.0%
!!! ATTTTATTTTTTTGAAAACT+CGG + chr7_3:80547593-80547612 None:intergenic 10.0%
!! AAATTCCAAATATATTAATA+TGG + chr7_3:80549536-80549555 None:intergenic 10.0%
!! TTCAGGTAATTAAAATAAAA+TGG + chr7_3:80547186-80547205 None:intergenic 15.0%
!! ATAACAATTACATATAATTC+AGG + chr7_3:80547203-80547222 None:intergenic 15.0%
!! TTCATTGTATTTATTTCATA+AGG - chr7_3:80549426-80549445 Msa1128630:intron 15.0%
!! TTTGACCATATTAATATATT+TGG - chr7_3:80549528-80549547 Msa1128630:five_prime_UTR 15.0%
!!! AATTGTTATGATTCTTTTGA+AGG - chr7_3:80547214-80547233 Msa1128630:three_prime_UTR 20.0%
!! AAAACAAGTATTTAGCAAAT+AGG - chr7_3:80548461-80548480 Msa1128630:intron 20.0%
!! AAACAAGTATTTAGCAAATA+GGG - chr7_3:80548462-80548481 Msa1128630:intron 20.0%
!! AAATATGTACAAAAAGTTGA+TGG - chr7_3:80549316-80549335 Msa1128630:CDS 20.0%
!!! ATATGTTCTTAAATTTTGGA+CGG + chr7_3:80549390-80549409 None:intergenic 20.0%
!!! CACAATATGTTCTTAAATTT+TGG + chr7_3:80549394-80549413 None:intergenic 20.0%
! ATGTTATGTTGCTAATTTGA+AGG - chr7_3:80547648-80547667 Msa1128630:three_prime_UTR 25.0%
! GAATTGAGAATAAAGAATTG+AGG + chr7_3:80548079-80548098 None:intergenic 25.0%
!! AACTGTTGTAGAAATTTTAC+AGG - chr7_3:80548180-80548199 Msa1128630:CDS 25.0%
!!! TCAAAGTCTCTTACTTTTTT+TGG + chr7_3:80548427-80548446 None:intergenic 25.0%
! AAGAAGTTGAAGAGAATAAA+GGG + chr7_3:80548537-80548556 None:intergenic 25.0%
!! GATTTGGTTATAAACTTATG+CGG + chr7_3:80548789-80548808 None:intergenic 25.0%
!! TATGTTCTTAAATTTTGGAC+GGG + chr7_3:80549389-80549408 None:intergenic 25.0%
!! TGTTATGATTCTTTTGAAGG+TGG - chr7_3:80547217-80547236 Msa1128630:three_prime_UTR 30.0%
TTTCCCAAACCAGTTAATTT+AGG - chr7_3:80547710-80547729 Msa1128630:intron 30.0%
ACTAAGCTATTATTCACGAT+TGG - chr7_3:80547963-80547982 Msa1128630:CDS 30.0%
ACCAATTACATTGAAACGAT+TGG - chr7_3:80547987-80548006 Msa1128630:CDS 30.0%
TGAGAATAAAGAATTGAGGA+GGG + chr7_3:80548075-80548094 None:intergenic 30.0%
TTGAGAATAAAGAATTGAGG+AGG + chr7_3:80548076-80548095 None:intergenic 30.0%
AGAGAAATAGTTTCTTCAGT+TGG - chr7_3:80548217-80548236 Msa1128630:CDS 30.0%
!!! TTGTTTGAGTTTTGAGATAG+CGG + chr7_3:80548274-80548293 None:intergenic 30.0%
AAAACATACTCAGAGCTAAA+AGG - chr7_3:80548308-80548327 Msa1128630:CDS 30.0%
!!! AAGTCTCTTACTTTTTTTGG+AGG + chr7_3:80548424-80548443 None:intergenic 30.0%
GAAGAAGTTGAAGAGAATAA+AGG + chr7_3:80548538-80548557 None:intergenic 30.0%
! TGCTCTATTTCTTGATATGA+CGG + chr7_3:80548592-80548611 None:intergenic 30.0%
GTTGTTGATGTTACTCTATT+TGG + chr7_3:80548672-80548691 None:intergenic 30.0%
GATTCAATCAGAAACTTATG+TGG + chr7_3:80548720-80548739 None:intergenic 30.0%
TTATAAACTTATGCGGTGAT+TGG + chr7_3:80548782-80548801 None:intergenic 30.0%
! TGTTACTCTATTTGGTGATT+TGG + chr7_3:80548805-80548824 None:intergenic 30.0%
GTTGTTGATGTTACTCTATT+TGG + chr7_3:80548672-80548691 None:intergenic 30.0%
GATTCAATAAGAAACTTGTG+TGG + chr7_3:80548861-80548880 None:intergenic 30.0%
! GATTTGGTCATAAACTTATG+CGG + chr7_3:80548930-80548949 None:intergenic 30.0%
!!! TGATGTTACTTTTGGTGATT+TGG + chr7_3:80548946-80548965 None:intergenic 30.0%
GATTTGATCAGAAACTTATG+TGG + chr7_3:80548999-80549018 None:intergenic 30.0%
ACATATTTCTTGCTCTTCAA+TGG + chr7_3:80549304-80549323 None:intergenic 30.0%
! ATGTTCTTAAATTTTGGACG+GGG + chr7_3:80549388-80549407 None:intergenic 30.0%
CACATAGTAAGTAGTAACCA+TGG - chr7_3:80547051-80547070 Msa1128630:three_prime_UTR 35.0%
ACATATTACGTTGTCTAGGT+GGG + chr7_3:80547087-80547106 None:intergenic 35.0%
AACATATTACGTTGTCTAGG+TGG + chr7_3:80547088-80547107 None:intergenic 35.0%
GCTAACATATTACGTTGTCT+AGG + chr7_3:80547091-80547110 None:intergenic 35.0%
AGTGTTTGTCTGATAAGAAC+AGG + chr7_3:80547124-80547143 None:intergenic 35.0%
TATCAGACAAACACTCAACA+AGG - chr7_3:80547129-80547148 Msa1128630:three_prime_UTR 35.0%
! TAAGGGTGTGATTAAGGAAA+AGG + chr7_3:80547161-80547180 None:intergenic 35.0%
! ATGGATTAAGGGTGTGATTA+AGG + chr7_3:80547167-80547186 None:intergenic 35.0%
ACAAGAACTACAATTCACTG+TGG - chr7_3:80547259-80547278 Msa1128630:three_prime_UTR 35.0%
!! ACTTGTTCTGAGATAAGTTC+TGG + chr7_3:80547333-80547352 None:intergenic 35.0%
GATGCAAGCAAGAATCTATT+AGG - chr7_3:80547357-80547376 Msa1128630:three_prime_UTR 35.0%
TTGGCATCAATTAAGAACGA+AGG + chr7_3:80547474-80547493 None:intergenic 35.0%
! CCTCCTAAATTAACTGGTTT+GGG + chr7_3:80547716-80547735 None:intergenic 35.0%
ACCTCCTAAATTAACTGGTT+TGG + chr7_3:80547717-80547736 None:intergenic 35.0%
ACCAGTTAATTTAGGAGGTA+CGG - chr7_3:80547718-80547737 Msa1128630:intron 35.0%
! CTGGAACTTAGAATTGACTT+TGG + chr7_3:80547772-80547791 None:intergenic 35.0%
AGTTCCAGCACTTAAAATTG+TGG - chr7_3:80547784-80547803 Msa1128630:intron 35.0%
!! AACACCACAATTTTAAGTGC+TGG + chr7_3:80547791-80547810 None:intergenic 35.0%
AATCTTGATTGCACGAGATA+AGG + chr7_3:80547904-80547923 None:intergenic 35.0%
AATATGGTGAGAAAAAGGTC+CGG + chr7_3:80547933-80547952 None:intergenic 35.0%
GTAGCAATATGGTGAGAAAA+AGG + chr7_3:80547938-80547957 None:intergenic 35.0%
CTTAGTGACAAGTAGCAATA+TGG + chr7_3:80547949-80547968 None:intergenic 35.0%
GCCAATCGTTTCAATGTAAT+TGG + chr7_3:80547991-80548010 None:intergenic 35.0%
!! TTTGAGATAGCGGTGTTTTT+GGG + chr7_3:80548264-80548283 None:intergenic 35.0%
!!! TTTTGAGATAGCGGTGTTTT+TGG + chr7_3:80548265-80548284 None:intergenic 35.0%
!!! TAGCTCTGAGTATGTTTTCA+GGG + chr7_3:80548306-80548325 None:intergenic 35.0%
!!! TTAGCTCTGAGTATGTTTTC+AGG + chr7_3:80548307-80548326 None:intergenic 35.0%
GTTGAAGAGAATAAAGGGTT+GGG + chr7_3:80548532-80548551 None:intergenic 35.0%
AGTTGAAGAGAATAAAGGGT+TGG + chr7_3:80548533-80548552 None:intergenic 35.0%
GATTTGATCCTAAACTTGTG+TGG + chr7_3:80548624-80548643 None:intergenic 35.0%
GTTTCGATCAGAAACTTATG+AGG + chr7_3:80548648-80548667 None:intergenic 35.0%
TCAGAAACTTATGTGGTGAT+TGG + chr7_3:80548713-80548732 None:intergenic 35.0%
TAAGAAACTTGTGTGGTGAT+TGG + chr7_3:80548854-80548873 None:intergenic 35.0%
! TGTGTTGTTGCTACTCTATT+TGG + chr7_3:80548885-80548904 None:intergenic 35.0%
TCATAAACTTATGCGGTGAT+TGG + chr7_3:80548923-80548942 None:intergenic 35.0%
!!! GGTGTTGTTGATGTTACTTT+TGG + chr7_3:80548954-80548973 None:intergenic 35.0%
TTCAATCTTGTAGGTCTCTT+GGG + chr7_3:80549032-80549051 None:intergenic 35.0%
TTTGGAGAGTTCAATCTTGT+AGG + chr7_3:80549041-80549060 None:intergenic 35.0%
! AGAAGTGTACAGAAAAGTGA+TGG - chr7_3:80549134-80549153 Msa1128630:intron 35.0%
!! GATGGATTAGTGTGTTGAAT+AGG - chr7_3:80549152-80549171 Msa1128630:intron 35.0%
!! AAAGTTGATGGCTTTGTTAG+TGG - chr7_3:80549328-80549347 Msa1128630:CDS 35.0%
CATATTACGTTGTCTAGGTG+GGG + chr7_3:80547086-80547105 None:intergenic 40.0%
GAGAATGCGTTGAAAGCAAA+AGG + chr7_3:80547392-80547411 None:intergenic 40.0%
ATTGTCTTTACCCTCTCAGA+GGG - chr7_3:80547412-80547431 Msa1128630:three_prime_UTR 40.0%
! TTAAATAACGCCCTCTGAGA+GGG + chr7_3:80547425-80547444 None:intergenic 40.0%
ATTAAATAACGCCCTCTGAG+AGG + chr7_3:80547426-80547445 None:intergenic 40.0%
! TAATCTAGAGTTCCGTCGAA+AGG - chr7_3:80547442-80547461 Msa1128630:three_prime_UTR 40.0%
CCCAAACCAGTTAATTTAGG+AGG - chr7_3:80547713-80547732 Msa1128630:intron 40.0%
ACCGTACCTCCTAAATTAAC+TGG + chr7_3:80547722-80547741 None:intergenic 40.0%
ATCTCGTGCAATCAAGATTG+CGG - chr7_3:80547905-80547924 Msa1128630:three_prime_UTR 40.0%
ACTCAGAGCTAAAAGGAGAA+AGG - chr7_3:80548315-80548334 Msa1128630:CDS 40.0%
!! ACTTGTTGAGATGGTAATGG+TGG + chr7_3:80548373-80548392 None:intergenic 40.0%
! AGAGACCTTGAAGTTGATTC+TGG + chr7_3:80548401-80548420 None:intergenic 40.0%
! TAGCAAATAGGGTATCTCCT+AGG - chr7_3:80548473-80548492 Msa1128630:intron 40.0%
! ATAGGGTATCTCCTAGGAAT+AGG - chr7_3:80548479-80548498 Msa1128630:intron 40.0%
CAACAACACCACACAAGTTT+CGG - chr7_3:80548685-80548704 Msa1128630:intron 40.0%
TGCGTTGTTGCTACTCTATT+TGG + chr7_3:80548744-80548763 None:intergenic 40.0%
CAACAACGCAACACAAGTTT+CGG - chr7_3:80548754-80548773 Msa1128630:intron 40.0%
CAACAACACCACACAAGTTT+CGG - chr7_3:80548685-80548704 Msa1128630:intron 40.0%
CAACAACACAACCCAAGTTT+CGG - chr7_3:80548895-80548914 Msa1128630:CDS 40.0%
CAACAACACCACAAAAGTCT+CGG - chr7_3:80548964-80548983 Msa1128630:intron 40.0%
! GATTCAATCCGAGACTTTTG+TGG + chr7_3:80548975-80548994 None:intergenic 40.0%
GTTCAATCTTGTAGGTCTCT+TGG + chr7_3:80549033-80549052 None:intergenic 40.0%
ACCTTTGTCTCCTTCTAGAT+TGG - chr7_3:80549091-80549110 Msa1128630:intron 40.0%
ACACTTCTCTTGCTCTTTGT+TGG + chr7_3:80549122-80549141 None:intergenic 40.0%
!! CCATCACTTTTCTTGCTCTT+TGG + chr7_3:80549184-80549203 None:intergenic 40.0%
CCAAAGAGCAAGAAAAGTGA+TGG - chr7_3:80549181-80549200 Msa1128630:intron 40.0%
!! GAGCAAGAAAAGTGATGGAT+TGG - chr7_3:80549186-80549205 Msa1128630:intron 40.0%
CAAGAGTTCTCCAAAGAGAT+TGG - chr7_3:80549219-80549238 Msa1128630:CDS 40.0%
TTTGATGCAGCCAATCTCTT+TGG + chr7_3:80549232-80549251 None:intergenic 40.0%
CTTACTATGTGTGATCCACG+CGG + chr7_3:80547042-80547061 None:intergenic 45.0%
TTTGAAGGTGGATCAGTGCA+TGG - chr7_3:80547229-80547248 Msa1128630:three_prime_UTR 45.0%
GAACTACAATTCACTGTGGC+AGG - chr7_3:80547263-80547282 Msa1128630:three_prime_UTR 45.0%
!! AGATAAGTTCTGGTGCTACG+AGG + chr7_3:80547323-80547342 None:intergenic 45.0%
CATTGTCTTTACCCTCTCAG+AGG - chr7_3:80547411-80547430 Msa1128630:three_prime_UTR 45.0%
CGAAGGAACAATCCTTTCGA+CGG + chr7_3:80547457-80547476 None:intergenic 45.0%
CCAAATTAGCCCACCGAAAT+TGG + chr7_3:80547505-80547524 None:intergenic 45.0%
!! CCAATTTCGGTGGGCTAATT+TGG - chr7_3:80547502-80547521 Msa1128630:three_prime_UTR 45.0%
! TGGGCTAATTTGGCTTCTTC+AGG - chr7_3:80547512-80547531 Msa1128630:three_prime_UTR 45.0%
TGCACAAAAAACATCTCCGC+TGG - chr7_3:80547682-80547701 Msa1128630:three_prime_UTR 45.0%
GCACAAAAAACATCTCCGCT+GGG - chr7_3:80547683-80547702 Msa1128630:three_prime_UTR 45.0%
CACAAAAAACATCTCCGCTG+GGG - chr7_3:80547684-80547703 Msa1128630:three_prime_UTR 45.0%
TGTGTTTGATGCAGCCTCAA+TGG - chr7_3:80547847-80547866 Msa1128630:intron 45.0%
CGATTGGCATTCGTGAGTTT+CGG - chr7_3:80548003-80548022 Msa1128630:CDS 45.0%
GATTGGCATTCGTGAGTTTC+GGG - chr7_3:80548004-80548023 Msa1128630:CDS 45.0%
GTAACTTTGATGCAGCTGAG+TGG - chr7_3:80548122-80548141 Msa1128630:CDS 45.0%
TCATTGCCTGCAATGCTTGT+AGG - chr7_3:80548154-80548173 Msa1128630:CDS 45.0%
! GTTTCACCTACAAGCATTGC+AGG + chr7_3:80548163-80548182 None:intergenic 45.0%
TGGTACAAAATTTGCCACCG+AGG - chr7_3:80548237-80548256 Msa1128630:CDS 45.0%
CAGAGCTAAAAGGAGAAAGG+AGG - chr7_3:80548318-80548337 Msa1128630:CDS 45.0%
! CGCACTTGTTGAGATGGTAA+TGG + chr7_3:80548376-80548395 None:intergenic 45.0%
GCGTTCCAGAATCAACTTCA+AGG - chr7_3:80548393-80548412 Msa1128630:CDS 45.0%
TATCTCCTAGGAATAGGCCA+TGG - chr7_3:80548485-80548504 Msa1128630:intron 45.0%
ATCTCCTAGGAATAGGCCAT+GGG - chr7_3:80548486-80548505 Msa1128630:intron 45.0%
! AGTACTGTCTTTCTAGCCCA+TGG + chr7_3:80548505-80548524 None:intergenic 45.0%
CAACAACGCCACACAAGTTT+AGG - chr7_3:80548613-80548632 Msa1128630:intron 45.0%
GATTGGATCCGAAACTTGTG+TGG + chr7_3:80548696-80548715 None:intergenic 45.0%
GATTGGATCCGAAACTTGTG+TGG + chr7_3:80548696-80548715 None:intergenic 45.0%
GGTGATTGGATCCGAAACTT+GGG + chr7_3:80548699-80548718 None:intergenic 45.0%
GATGCAGCACGATATCTCTT+TGG + chr7_3:80549059-80549078 None:intergenic 45.0%
GCCAATCTAGAAGGAGACAA+AGG + chr7_3:80549095-80549114 None:intergenic 45.0%
ATCTTTCTCTCCTTCGAGAC+TGG - chr7_3:80549261-80549280 Msa1128630:CDS 45.0%
GGCAATTGTACTGTTGTAGC+TGG + chr7_3:80549367-80549386 None:intergenic 45.0%
! ACGTTGTCTAGGTGGGGTTT+TGG + chr7_3:80547080-80547099 None:intergenic 50.0%
AGGTGGATCAGTGCATGGAT+CGG - chr7_3:80547234-80547253 Msa1128630:three_prime_UTR 50.0%
CTACAATTCACTGTGGCAGG+TGG - chr7_3:80547266-80547285 Msa1128630:three_prime_UTR 50.0%
! GGTGGAACAAAGCTGGTTTC+TGG - chr7_3:80547284-80547303 Msa1128630:three_prime_UTR 50.0%
GCCAACCCAGATGCCAATTT+CGG - chr7_3:80547489-80547508 Msa1128630:three_prime_UTR 50.0%
ACCGAAATTGGCATCTGGGT+TGG + chr7_3:80547493-80547512 None:intergenic 50.0%
AACCCAGATGCCAATTTCGG+TGG - chr7_3:80547492-80547511 Msa1128630:three_prime_UTR 50.0%
ACCCAGATGCCAATTTCGGT+GGG - chr7_3:80547493-80547512 Msa1128630:three_prime_UTR 50.0%
GAGACAGAGAGAGCAAGCAA+AGG + chr7_3:80547746-80547765 None:intergenic 50.0%
CCACAACGTTACTGCCATTG+AGG + chr7_3:80547864-80547883 None:intergenic 50.0%
CCTCAATGGCAGTAACGTTG+TGG - chr7_3:80547861-80547880 Msa1128630:intron 50.0%
TGCAATCAAGATTGCGGCAC+CGG - chr7_3:80547911-80547930 Msa1128630:exon 50.0%
!!! TAGCGGTGTTTTTGGGTCCT+CGG + chr7_3:80548257-80548276 None:intergenic 50.0%
! CTGGAACGCACTTGTTGAGA+TGG + chr7_3:80548382-80548401 None:intergenic 50.0%
CGGTGATTGGATCCGAAACT+TGG + chr7_3:80548769-80548788 None:intergenic 50.0%
CTTGTAGGTCTCTTGGGTGA+AGG + chr7_3:80549026-80549045 None:intergenic 50.0%
GTTGGTGCAGCCAATCTAGA+AGG + chr7_3:80549104-80549123 None:intergenic 50.0%
CTCCTTCGAGACTGGTAACA+AGG - chr7_3:80549269-80549288 Msa1128630:CDS 50.0%
!! AGCCTTGTTACCAGTCTCGA+AGG + chr7_3:80549274-80549293 None:intergenic 50.0%
ACGTTACGTTAGGATCCGCG+TGG - chr7_3:80547024-80547043 Msa1128630:three_prime_UTR 55.0%
!! AGGTGGGGTTTTGGCAACCA+TGG + chr7_3:80547071-80547090 None:intergenic 55.0%
TGTGGCAGGTGGAACAAAGC+TGG - chr7_3:80547277-80547296 Msa1128630:three_prime_UTR 55.0%
GCCCACCGAAATTGGCATCT+GGG + chr7_3:80547497-80547516 None:intergenic 55.0%
AGCCCACCGAAATTGGCATC+TGG + chr7_3:80547498-80547517 None:intergenic 55.0%
GGTTTGGGAAACTTCCCCAG+CGG + chr7_3:80547701-80547720 None:intergenic 55.0%
CTAGCCCATGGCCTATTCCT+AGG + chr7_3:80548493-80548512 None:intergenic 55.0%
GTAGGTCTCTTGGGTGAAGG+TGG + chr7_3:80549023-80549042 None:intergenic 55.0%
!!! CGGTGTTTTTGGGTCCTCGG+TGG + chr7_3:80548254-80548273 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr7_3 gene 80547020 80549567 80547020 ID=Msa1128630;Name=Msa1128630
chr7_3 mRNA 80547020 80549567 80547020 ID=Msa1128630-mRNA-1;Parent=Msa1128630;Name=Msa1128630-mRNA-1;_AED=0.06;_eAED=0.06;_QI=50|1|1|1|0.75|0.6|5|766|270
chr7_3 exon 80547020 80547725 80547020 ID=Msa1128630-mRNA-1:exon:23099;Parent=Msa1128630-mRNA-1
chr7_3 exon 80547867 80548450 80547867 ID=Msa1128630-mRNA-1:exon:23098;Parent=Msa1128630-mRNA-1
chr7_3 exon 80548853 80548917 80548853 ID=Msa1128630-mRNA-1:exon:23097;Parent=Msa1128630-mRNA-1
chr7_3 exon 80549209 80549351 80549209 ID=Msa1128630-mRNA-1:exon:23096;Parent=Msa1128630-mRNA-1
chr7_3 exon 80549437 80549567 80549437 ID=Msa1128630-mRNA-1:exon:23095;Parent=Msa1128630-mRNA-1
chr7_3 five_prime_UTR 80549518 80549567 80549518 ID=Msa1128630-mRNA-1:five_prime_utr;Parent=Msa1128630-mRNA-1
chr7_3 CDS 80549437 80549517 80549437 ID=Msa1128630-mRNA-1:cds;Parent=Msa1128630-mRNA-1
chr7_3 CDS 80549209 80549351 80549209 ID=Msa1128630-mRNA-1:cds;Parent=Msa1128630-mRNA-1
chr7_3 CDS 80548853 80548917 80548853 ID=Msa1128630-mRNA-1:cds;Parent=Msa1128630-mRNA-1
chr7_3 CDS 80547927 80548450 80547927 ID=Msa1128630-mRNA-1:cds;Parent=Msa1128630-mRNA-1
chr7_3 three_prime_UTR 80547867 80547926 80547867 ID=Msa1128630-mRNA-1:three_prime_utr;Parent=Msa1128630-mRNA-1
chr7_3 three_prime_UTR 80547020 80547725 80547020 ID=Msa1128630-mRNA-1:three_prime_utr;Parent=Msa1128630-mRNA-1
Gene Sequence

>Msa1128630

ATGGTTGCCAAAACCCCACCTAGACAACGTAATATGTTAGCTGTTCTAAATCCTGTTCTTATCAGACAAACACTCAACAAGGTGGATCAGTGCATGGATCGGCTACAAGAACTACAATTCACTGTGGCAGGTGGAACAAAGCTGGTTTCTGGAGTTAATCTAAGTCCTCGTAGCACCAGAACTTATCTCAGAACAAGTCTAAGATGCAAGCAAGAATCTATTAGGATCAAGAATAGTGCACAAAAAACATCTCCGCTGGGGAAGTTTCCCAAACCAGTTAATTTAGGAGCTGAGTGGAAGCAAATGTCATTGCCTGCAATGCTTGTAGGTGAAACTGTTGTAGAAATTTTACAGGCAAGTCAATTTGCAAGAGAAATAGTTTCTTCAGTTGGTACAAAATTTGCCACCGAGGACCCAAAAACACCGCTATCTCAAAACTCAAACAAAAAAGCAGACCCTGAAAACATACTCAGAGCTAAAAGGAGAAAGGAGGAGAAGCAAAATAAACAACAATCTGATTCGCCACCATTACCATCTCAACAAGTGCGTTCCAGAATCAACTTCAAGGTCTCTCCTCCAAAAAAAGTAAGAGACTTTGATAAAGAGAAAGAGAAAAACAAGTATTTAGCAAATAGGGTATCTCCTAGGAATAGGCCATGGGCTAGAAAGACAGTACTTTTTCCCAACCCTTTATTCTCTTCAACTTCTTCACATCATAAACAATTTTGCAAGACAAGATCACCGTCATATCAAGAAATAGAGCAACAACAACGCCACACAAGTTTAGGATCAAATCACCTCATAAGTTTCTGA
Protein sequence

>Msa1128630

MVAKTPPRQRNMLAVLNPVLIRQTLNKVDQCMDRLQELQFTVAGGTKLVSGVNLSPRSTRTYLRTSLRCKQESIRIKNSAQKTSPLGKFPKPVNLGAEWKQMSLPAMLVGETVVEILQASQFAREIVSSVGTKFATEDPKTPLSQNSNKKADPENILRAKRRKEEKQNKQQSDSPPLPSQQVRSRINFKVSPPKKVRDFDKEKEKNKYLANRVSPRNRPWARKTVLFPNPLFSSTSSHHKQFCKTRSPSYQEIEQQQRHTSLGSNHLISF*