AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1131190

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1131190 MtrunA17_Chr7g0272991 99.615 260 1 0 1 260 1 260 0.0 537
Msa1131190 MtrunA17_Chr1g0191681 83.539 243 36 1 1 243 1 239 3.62e-140 394
Msa1131190 MtrunA17_Chr2g0310741 55.785 242 92 7 12 247 15 247 9.87e-76 231
Msa1131190 MtrunA17_Chr3g0132191 66.038 159 39 4 83 238 37 183 1.24e-62 196
Msa1131190 MtrunA17_Chr2g0288481 49.468 188 70 8 82 259 66 238 6.82e-53 172
Msa1131190 MtrunA17_Chr2g0318881 59.770 87 34 1 100 185 21 107 1.64e-31 112
Msa1131190 MtrunA17_Chr2g0318921 61.446 83 31 1 100 181 21 103 6.70e-31 111
Msa1131190 MtrunA17_Chr8g0376351 61.333 75 28 1 119 192 1 75 2.16e-27 101
Msa1131190 MtrunA17_Chr3g0081161 39.744 78 47 0 102 179 9 86 7.89e-12 63.9
Msa1131190 MtrunA17_Chr8g0354151 37.179 78 49 0 102 179 9 86 1.47e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1131190 AT1G08970.3 62.500 248 63 8 1 247 1 219 4.92e-88 261
Msa1131190 AT1G08970.4 62.500 248 63 8 1 247 1 219 4.92e-88 261
Msa1131190 AT1G08970.2 62.500 248 63 8 1 247 1 219 4.92e-88 261
Msa1131190 AT1G08970.1 62.500 248 63 8 1 247 1 219 4.92e-88 261
Msa1131190 AT1G54830.2 63.636 242 56 5 16 256 5 215 2.92e-87 259
Msa1131190 AT1G54830.3 63.636 242 56 5 16 256 5 215 2.92e-87 259
Msa1131190 AT1G54830.1 63.636 242 56 5 16 256 5 215 2.92e-87 259
Msa1131190 AT3G48590.1 68.790 157 44 3 83 238 47 199 1.76e-70 216
Msa1131190 AT5G63470.2 68.987 158 45 4 83 238 60 215 2.07e-67 209
Msa1131190 AT5G63470.1 68.987 158 45 4 83 238 60 215 2.07e-67 209
Msa1131190 AT1G56170.1 75.758 132 25 3 80 209 55 181 1.35e-66 205
Msa1131190 AT1G56170.2 75.758 132 25 3 80 209 55 181 1.35e-66 205
Msa1131190 AT5G50480.1 49.123 171 63 5 79 248 35 182 2.74e-46 154
Msa1131190 AT5G27910.1 45.349 172 72 8 77 248 15 164 4.68e-39 134
Msa1131190 AT5G50490.1 38.889 180 96 4 70 249 8 173 1.71e-34 123
Msa1131190 AT5G50470.1 39.560 182 71 8 79 251 44 195 9.78e-31 114
Msa1131190 AT5G38140.1 43.269 104 55 2 76 178 44 144 1.41e-25 100
Msa1131190 AT3G12480.1 37.179 78 49 0 102 179 9 86 1.30e-11 63.9

Find 123 sgRNAs with CRISPR-Local

Find 192 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGCTGGGTAAAATGTAATT+TGG 0.125571 7_3:+83721421 Msa1131190:three_prime_UTR
AAGCCAGCACTTCATCTTTC+AGG 0.158704 7_3:-83720766 None:intergenic
GTCATTTGGTTTGGCTGATA+TGG 0.158964 7_3:-83720302 None:intergenic
GACTGATATCTTTGATTTCC+TGG 0.246183 7_3:+83720721 Msa1131190:CDS
TAATTTGGGTTTAAACCTTA+TGG 0.256597 7_3:+83721436 Msa1131190:three_prime_UTR
TCAACCTGCTGGAGCTCAGC+TGG 0.263146 7_3:+83720376 Msa1131190:CDS
GCAGACTTCTATTGCCTATT+GGG 0.267661 7_3:+83721491 Msa1131190:three_prime_UTR
TGCAGACTTCTATTGCCTAT+TGG 0.280582 7_3:+83721490 Msa1131190:three_prime_UTR
ATCTTTATGTTTAATTAAGC+TGG 0.286907 7_3:+83721405 Msa1131190:three_prime_UTR
CTATTGGGTTATATTTACTA+TGG 0.312585 7_3:+83721506 Msa1131190:three_prime_UTR
GGGGCCCCAGTCATTTGGTT+TGG 0.316348 7_3:-83720311 None:intergenic
AGCTGGGTAAAATGTAATTT+GGG 0.319298 7_3:+83721422 Msa1131190:three_prime_UTR
TTTCCTGGCTTGCAGATTGC+AGG 0.326598 7_3:+83721269 Msa1131190:intron
GGAGATGGTGGTCGCTGTTC+CGG 0.343092 7_3:-83720974 None:intergenic
TAAAGGAATCATAGATTTGC+AGG 0.344712 7_3:-83721565 None:intergenic
GGCTGCTGAGCATACATGTT+TGG 0.345011 7_3:-83720914 None:intergenic
GCCTCCCAAGTTGGAACTGC+TGG 0.356093 7_3:+83720863 Msa1131190:CDS
ACATAAAGATAAGTTATTAC+TGG 0.361320 7_3:-83721392 None:intergenic
GATCCGTTGTTACATCAGTT+GGG 0.366704 7_3:+83720336 Msa1131190:CDS
ATCCTGGGGCCCCAGTCATT+TGG 0.379289 7_3:-83720316 None:intergenic
TCATTTGGTTTGGCTGATAT+GGG 0.380613 7_3:-83720301 None:intergenic
GGTTTAAACCTTATGGTCTT+CGG 0.383720 7_3:+83721443 Msa1131190:three_prime_UTR
ACTGATGTAACAACGGATCC+TGG 0.389880 7_3:-83720332 None:intergenic
TGGAACTGCTGGTGTCATAA+TGG 0.411463 7_3:+83720874 Msa1131190:CDS
CACAACCAGAATCCGCAAAT+GGG 0.411828 7_3:+83720239 Msa1131190:CDS
AGAGGAACAATGCCTGTTGC+TGG 0.417994 7_3:+83720797 Msa1131190:CDS
GGATCCGTTGTTACATCAGT+TGG 0.426256 7_3:+83720335 Msa1131190:CDS
CATAGTAAATATAACCCAAT+AGG 0.426321 7_3:-83721505 None:intergenic
AACAACAGCTGCAATCCTTC+TGG 0.435091 7_3:+83720453 Msa1131190:CDS
GCTGCTGAGCATACATGTTT+GGG 0.435445 7_3:-83720913 None:intergenic
CATCTCAGCATCTAGTGATC+TGG 0.437549 7_3:-83720995 None:intergenic
CAGTGTGGTTCCATGAGCGC+AGG 0.449372 7_3:-83720643 None:intergenic
ACAAATGTGGCCACAGCCTC+CGG 0.450073 7_3:+83720955 Msa1131190:CDS
GCACAACCAGAATCCGCAAA+TGG 0.451270 7_3:+83720238 Msa1131190:CDS
CAAGAACCACAGCCTTCCTC+TGG 0.452736 7_3:+83720514 Msa1131190:CDS
GCATCAGCAGGACCAGCAAC+AGG 0.455014 7_3:-83720809 None:intergenic
GTTGCTGCTGTTGCTGCTGA+TGG 0.455992 7_3:-83720427 None:intergenic
GGCCTGTGAGATGTTCATAT+TGG 0.456864 7_3:+83720613 Msa1131190:CDS
CCATGAGAGTTGACTGCTAA+AGG 0.458260 7_3:-83721582 None:intergenic
TGGAGTTAACCCTGCGCTCA+TGG 0.458619 7_3:+83720633 Msa1131190:CDS
CAGTCCACTGGTCAACCTGC+TGG 0.464075 7_3:+83720365 Msa1131190:CDS
CACATTTGTGGTGCCATGTA+GGG 0.465255 7_3:-83720941 None:intergenic
CTGATGTAACAACGGATCCT+GGG 0.469388 7_3:-83720331 None:intergenic
CTGGTACTGGTTTGACCAGA+AGG 0.471679 7_3:-83720468 None:intergenic
CAACCTGCTGGAGCTCAGCT+GGG 0.476224 7_3:+83720377 Msa1131190:CDS
ATGCTGAGATGTGGATGTTA+AGG 0.476601 7_3:+83721007 Msa1131190:three_prime_UTR
TGACACCAGCAGTTCCAACT+TGG 0.478083 7_3:-83720868 None:intergenic
GGAACTGCTGGTGTCATAAT+GGG 0.479046 7_3:+83720875 Msa1131190:CDS
TCTTTATGTTTAATTAAGCT+GGG 0.482086 7_3:+83721406 Msa1131190:three_prime_UTR
TTGTGGTGCCATGTAGGGAT+GGG 0.484954 7_3:-83720936 None:intergenic
AACCAAATGACTGGGGCCCC+AGG 0.486456 7_3:+83720314 Msa1131190:CDS
TTTGTGGTGCCATGTAGGGA+TGG 0.486986 7_3:-83720937 None:intergenic
GGAGGCTGTGGCCACATTTG+TGG 0.486993 7_3:-83720953 None:intergenic
GCAACAGGCATTGTTCCTCT+TGG 0.487666 7_3:-83720794 None:intergenic
TGGGTGTGAAACTGTTCCAT+TGG 0.491473 7_3:+83721306 Msa1131190:three_prime_UTR
ACCACACTGAAGAGAACAAA+AGG 0.492883 7_3:+83720657 Msa1131190:CDS
CCACATTTGTGGTGCCATGT+AGG 0.497501 7_3:-83720942 None:intergenic
TACATGTTTGGGTCCATCAC+AGG 0.504424 7_3:-83720902 None:intergenic
TATATCATCCGAAGACCATA+AGG 0.504471 7_3:-83721451 None:intergenic
TATCAGCCAAACCAAATGAC+TGG 0.504884 7_3:+83720305 Msa1131190:CDS
AGATTGCAGGGAACTGCAAT+GGG 0.507879 7_3:+83721282 Msa1131190:intron
CCTCAGCATGCCTCCCAAGT+TGG 0.514710 7_3:+83720854 Msa1131190:CDS
TCTTGATCCTTGCCAGAGGA+AGG 0.515769 7_3:-83720526 None:intergenic
GTTGCTGCTGATGGATATGC+TGG 0.517658 7_3:-83720418 None:intergenic
TCATGGTCTTCTACCCCATC+GGG 0.517791 7_3:+83721599 Msa1131190:three_prime_UTR
TGACCCAGCTGAGCTCCAGC+AGG 0.517937 7_3:-83720380 None:intergenic
CCAGTACCAGGAAATTGAAA+AGG 0.518655 7_3:+83720481 Msa1131190:CDS
CTTCTGGTCAAACCAGTACC+AGG 0.521956 7_3:+83720469 Msa1131190:CDS
CAGATTGCAGGGAACTGCAA+TGG 0.524973 7_3:+83721281 Msa1131190:intron
CTCATGGTCTTCTACCCCAT+CGG 0.525020 7_3:+83721598 Msa1131190:three_prime_UTR
TTCCTGGCTTGCAGATTGCA+GGG 0.525676 7_3:+83721270 Msa1131190:intron
CCTTTAGCAGTCAACTCTCA+TGG 0.529370 7_3:+83721582 Msa1131190:three_prime_UTR
ATCCGTTGTTACATCAGTTG+GGG 0.531831 7_3:+83720337 Msa1131190:CDS
GCAGGGAACTGCAATGGGGT+GGG 0.534268 7_3:+83721287 Msa1131190:three_prime_UTR
GGTCGCTGTTCCGGAGGCTG+TGG 0.539229 7_3:-83720965 None:intergenic
GGACCTGAAAGATGAAGTGC+TGG 0.542953 7_3:+83720763 Msa1131190:CDS
TGTGGTGCCATGTAGGGATG+GGG 0.546062 7_3:-83720935 None:intergenic
ACTTGGGAGGCATGCTGAGG+TGG 0.548128 7_3:-83720851 None:intergenic
ATCAGCCAAACCAAATGACT+GGG 0.550264 7_3:+83720306 Msa1131190:CDS
GACACCAGCAGTTCCAACTT+GGG 0.552393 7_3:-83720867 None:intergenic
AAGCACCTGTCATATTTGCA+AGG 0.553287 7_3:+83720591 Msa1131190:CDS
CAGTAAGGAAGAGCATCAGC+AGG 0.560205 7_3:-83720821 None:intergenic
CAGGCCCTTGCAAATATGAC+AGG 0.560856 7_3:-83720596 None:intergenic
TCAGCAGCCCCATCCCTACA+TGG 0.562305 7_3:+83720928 Msa1131190:CDS
TGCAGGGAACTGCAATGGGG+TGG 0.562515 7_3:+83721286 Msa1131190:three_prime_UTR
ATCAGAAACTGTAGCTGAGA+TGG 0.564284 7_3:+83721333 Msa1131190:three_prime_UTR
TCCTTGCCAGAGGAAGGCTG+TGG 0.564697 7_3:-83720520 None:intergenic
GGAATCATAGATTTGCAGGC+AGG 0.566600 7_3:-83721561 None:intergenic
CAAAACAGAATGGATCATCA+AGG 0.570283 7_3:+83720215 Msa1131190:intron
AGCATCTAGTGATCTGGAGA+TGG 0.574668 7_3:-83720989 None:intergenic
ATATTGCTGCTGCAATCACT+AGG 0.575052 7_3:+83720699 Msa1131190:CDS
GAAGATCATGAAGGCTGATG+AGG 0.575398 7_3:+83720547 Msa1131190:CDS
TGAGGTGGCATGTAGCAGTA+AGG 0.584291 7_3:-83720836 None:intergenic
ACCACAGCCTTCCTCTGGCA+AGG 0.585880 7_3:+83720519 Msa1131190:CDS
ACAGTTTCTGATCGCACCAA+TGG 0.588674 7_3:-83721322 None:intergenic
AGCTCCAGCAGGTTGACCAG+TGG 0.593284 7_3:-83720369 None:intergenic
CATAATGGGAAAGCCTGTGA+TGG 0.593370 7_3:+83720889 Msa1131190:CDS
CACGAGGCACAATGTCAACC+AGG 0.593690 7_3:-83720739 None:intergenic
ACAACCAGAATCCGCAAATG+GGG 0.594889 7_3:+83720240 Msa1131190:CDS
ATCTAGTGATCTGGAGATGG+TGG 0.605869 7_3:-83720986 None:intergenic
CTCCAATATGAACATCTCAC+AGG 0.609517 7_3:-83720615 None:intergenic
TGATATGGGTTAGATCCGTA+CGG 0.617785 7_3:-83720287 None:intergenic
AGCGGCAGTCAAATGCCGTA+CGG 0.619913 7_3:+83720272 Msa1131190:CDS
TGATGTAACAACGGATCCTG+GGG 0.620797 7_3:-83720330 None:intergenic
AGCACCTGTCATATTTGCAA+GGG 0.622434 7_3:+83720592 Msa1131190:CDS
ATCTTCTTGATCCTTGCCAG+AGG 0.624577 7_3:-83720530 None:intergenic
AAAACAGAATGGATCATCAA+GGG 0.625548 7_3:+83720216 Msa1131190:intron
ACCAGCAGTTCCAACTTGGG+AGG 0.637100 7_3:-83720864 None:intergenic
AGTGTGGTTCCATGAGCGCA+GGG 0.637592 7_3:-83720642 None:intergenic
GATCACTAGATGCTGAGATG+TGG 0.638929 7_3:+83720998 Msa1131190:exon
CCTACATGGCACCACAAATG+TGG 0.644640 7_3:+83720942 Msa1131190:CDS
GATGGTGGTCGCTGTTCCGG+AGG 0.649506 7_3:-83720971 None:intergenic
CCAACTTGGGAGGCATGCTG+AGG 0.657795 7_3:-83720854 None:intergenic
TCATCTTTCAGGTCCTCACG+AGG 0.665344 7_3:-83720755 None:intergenic
AATTCCTTCTATTCCCGATG+GGG 0.666462 7_3:-83721612 None:intergenic
AAGGATCAAGAAGATCATGA+AGG 0.667741 7_3:+83720538 Msa1131190:CDS
TCCGTTGTTACATCAGTTGG+GGG 0.670764 7_3:+83720338 Msa1131190:CDS
GGTTGACATTGTGCCTCGTG+AGG 0.672349 7_3:+83720742 Msa1131190:CDS
CAACCAGAATCCGCAAATGG+GGG 0.676007 7_3:+83720241 Msa1131190:CDS
ACACTGAAGAGAACAAAAGG+AGG 0.682027 7_3:+83720660 Msa1131190:CDS
GTGCTGGCTTCAATTCCAAG+AGG 0.714242 7_3:+83720779 Msa1131190:CDS
TCAGCCAAACCAAATGACTG+GGG 0.740826 7_3:+83720307 Msa1131190:CDS
GATTGCAGGGAACTGCAATG+GGG 0.746367 7_3:+83721283 Msa1131190:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAAAAAACATCAAAAA+AGG - chr7_3:83719588-83719607 None:intergenic 10.0%
!!! TGATTATTGTTTTTCTTTTT+TGG + chr7_3:83719456-83719475 Msa1131190:intron 15.0%
!!! TGTAAATTGAAAACTTATTT+TGG + chr7_3:83719778-83719797 Msa1131190:intron 15.0%
!!! TTTCATTTTCAAAGAAAAGA+AGG + chr7_3:83719001-83719020 Msa1131190:intron 20.0%
!! AAGAAGAGAAAAAAAGTTTA+GGG - chr7_3:83719105-83719124 None:intergenic 20.0%
!! AAAGAAGAGAAAAAAAGTTT+AGG - chr7_3:83719106-83719125 None:intergenic 20.0%
!!! TAAACTTTTTTTCTCTTCTT+TGG + chr7_3:83719105-83719124 Msa1131190:intron 20.0%
!!! AAACTTTTTTTCTCTTCTTT+GGG + chr7_3:83719106-83719125 Msa1131190:intron 20.0%
!! ATACAGAAAAAAAAATGAAC+TGG - chr7_3:83719328-83719347 None:intergenic 20.0%
!! TCAACAGAACAAAAATTATT+CGG - chr7_3:83719664-83719683 None:intergenic 20.0%
!!! AAACTTATTTTGGTATTCTA+AGG + chr7_3:83719788-83719807 Msa1131190:intron 20.0%
!!! AACTTATTTTGGTATTCTAA+GGG + chr7_3:83719789-83719808 Msa1131190:intron 20.0%
!! CTAATAAAATAGCATACAAA+AGG - chr7_3:83721130-83721149 None:intergenic 20.0%
!!! TTTTATTAGTTAGCTTGATT+AGG + chr7_3:83721141-83721160 Msa1131190:intron 20.0%
!! ACATAAAGATAAGTTATTAC+TGG - chr7_3:83721395-83721414 None:intergenic 20.0%
!! ATCTTTATGTTTAATTAAGC+TGG + chr7_3:83721405-83721424 Msa1131190:three_prime_UTR 20.0%
!! TCTTTATGTTTAATTAAGCT+GGG + chr7_3:83721406-83721425 Msa1131190:three_prime_UTR 20.0%
!!! TCATTTTTTGTTTCTCTGAA+TGG + chr7_3:83719617-83719636 Msa1131190:intron 25.0%
!! GATTTTATGTCAAAACAGAA+TGG + chr7_3:83720205-83720224 Msa1131190:intron 25.0%
!! TAATTTGGGTTTAAACCTTA+TGG + chr7_3:83721436-83721455 Msa1131190:three_prime_UTR 25.0%
! CATAGTAAATATAACCCAAT+AGG - chr7_3:83721508-83721527 None:intergenic 25.0%
! CTATTGGGTTATATTTACTA+TGG + chr7_3:83721506-83721525 Msa1131190:three_prime_UTR 25.0%
! GAGTTGTTATGATAATGCTT+TGG - chr7_3:83719145-83719164 None:intergenic 30.0%
AAAACTGAGAACCTGAAAAA+GGG - chr7_3:83719256-83719275 None:intergenic 30.0%
GAATGGAGAATACATATTAC+TGG + chr7_3:83719634-83719653 Msa1131190:intron 30.0%
!! GAAGATTTGTTGATCAAATC+TGG + chr7_3:83719811-83719830 Msa1131190:intron 30.0%
GATTTATGCATGTCATGAAA+TGG + chr7_3:83719918-83719937 Msa1131190:intron 30.0%
GTTTAATGCATAGGAAAAAC+GGG - chr7_3:83720070-83720089 None:intergenic 30.0%
CGTTTAATGCATAGGAAAAA+CGG - chr7_3:83720071-83720090 None:intergenic 30.0%
! CGTTTTTCCTATGCATTAAA+CGG + chr7_3:83720069-83720088 Msa1131190:intron 30.0%
AACAAATCATGCAATCATTG+AGG - chr7_3:83720147-83720166 None:intergenic 30.0%
CATAAAATCAAACACGTGAT+CGG - chr7_3:83720194-83720213 None:intergenic 30.0%
AAAACAGAATGGATCATCAA+GGG + chr7_3:83720216-83720235 Msa1131190:intron 30.0%
TCCTTATACTTGATCTTTGA+TGG + chr7_3:83721192-83721211 Msa1131190:intron 30.0%
ACCATCAAAGATCAAGTATA+AGG - chr7_3:83721196-83721215 None:intergenic 30.0%
! CGCACATTTACTGTTATTTA+TGG + chr7_3:83721216-83721235 Msa1131190:intron 30.0%
AAGGCAATTTCAGACATAAA+AGG - chr7_3:83721247-83721266 None:intergenic 30.0%
AAGCTGGGTAAAATGTAATT+TGG + chr7_3:83721421-83721440 Msa1131190:three_prime_UTR 30.0%
AGCTGGGTAAAATGTAATTT+GGG + chr7_3:83721422-83721441 Msa1131190:three_prime_UTR 30.0%
TAAAGGAATCATAGATTTGC+AGG - chr7_3:83721568-83721587 None:intergenic 30.0%
!! GATTGATGATGGTGTTTGTT+TGG - chr7_3:83719045-83719064 None:intergenic 35.0%
GTTGTTGTGAAGATTGATGA+TGG - chr7_3:83719056-83719075 None:intergenic 35.0%
!! TCTTGTTTGATCCCTTTTTC+AGG + chr7_3:83719242-83719261 Msa1131190:intron 35.0%
GAAAACTGAGAACCTGAAAA+AGG - chr7_3:83719257-83719276 None:intergenic 35.0%
!!! AACCAAGCTTCGTTTTTTGA+GGG + chr7_3:83719281-83719300 Msa1131190:five_prime_UTR 35.0%
TTCATAGTTATGCTGTAACG+TGG + chr7_3:83719381-83719400 Msa1131190:intron 35.0%
AAATTCTTAGCTTCCATCAC+AGG - chr7_3:83719527-83719546 None:intergenic 35.0%
TTCGAGATACAATTGTGCAT+GGG + chr7_3:83719866-83719885 Msa1131190:intron 35.0%
AAGCTCAGGAGAAAAATAAC+AGG - chr7_3:83719949-83719968 None:intergenic 35.0%
CAACATTTCACAAACGACAA+AGG - chr7_3:83720171-83720190 None:intergenic 35.0%
CAAAACAGAATGGATCATCA+AGG + chr7_3:83720215-83720234 Msa1131190:intron 35.0%
!! TCATTTGGTTTGGCTGATAT+GGG - chr7_3:83720304-83720323 None:intergenic 35.0%
! CAGTAACCTTTTCAATTTCC+TGG - chr7_3:83720490-83720509 None:intergenic 35.0%
AAGGATCAAGAAGATCATGA+AGG + chr7_3:83720538-83720557 Msa1131190:CDS 35.0%
! GACTGATATCTTTGATTTCC+TGG + chr7_3:83720721-83720740 Msa1131190:CDS 35.0%
GGTTTAAACCTTATGGTCTT+CGG + chr7_3:83721443-83721462 Msa1131190:three_prime_UTR 35.0%
TATATCATCCGAAGACCATA+AGG - chr7_3:83721454-83721473 None:intergenic 35.0%
AATCGTGAGAAACGAGAGAA+TGG - chr7_3:83719221-83719240 None:intergenic 40.0%
!!! GAACCAAGCTTCGTTTTTTG+AGG + chr7_3:83719280-83719299 Msa1131190:five_prime_UTR 40.0%
TTCCCTCAAAAAACGAAGCT+TGG - chr7_3:83719286-83719305 None:intergenic 40.0%
!!! AAGCTTCGTTTTTTGAGGGA+AGG + chr7_3:83719285-83719304 Msa1131190:five_prime_UTR 40.0%
CTAAAAGACCTGTTCACACA+AGG - chr7_3:83719730-83719749 None:intergenic 40.0%
GTTCGAGATACAATTGTGCA+TGG + chr7_3:83719865-83719884 Msa1131190:intron 40.0%
! TCGTTTTCGATCATGACACA+CGG + chr7_3:83719972-83719991 Msa1131190:intron 40.0%
AGCGTAACCGTTTAATGCAT+AGG - chr7_3:83720079-83720098 None:intergenic 40.0%
!! TGATATGGGTTAGATCCGTA+CGG - chr7_3:83720290-83720309 None:intergenic 40.0%
!! GTCATTTGGTTTGGCTGATA+TGG - chr7_3:83720305-83720324 None:intergenic 40.0%
TATCAGCCAAACCAAATGAC+TGG + chr7_3:83720305-83720324 Msa1131190:CDS 40.0%
ATCAGCCAAACCAAATGACT+GGG + chr7_3:83720306-83720325 Msa1131190:CDS 40.0%
GATCCGTTGTTACATCAGTT+GGG + chr7_3:83720336-83720355 Msa1131190:CDS 40.0%
ATCCGTTGTTACATCAGTTG+GGG + chr7_3:83720337-83720356 Msa1131190:CDS 40.0%
! CCTTTTCAATTTCCTGGTAC+TGG - chr7_3:83720484-83720503 None:intergenic 40.0%
CCAGTACCAGGAAATTGAAA+AGG + chr7_3:83720481-83720500 Msa1131190:CDS 40.0%
AAGCACCTGTCATATTTGCA+AGG + chr7_3:83720591-83720610 Msa1131190:CDS 40.0%
AGCACCTGTCATATTTGCAA+GGG + chr7_3:83720592-83720611 Msa1131190:CDS 40.0%
CTCCAATATGAACATCTCAC+AGG - chr7_3:83720618-83720637 None:intergenic 40.0%
ACCACACTGAAGAGAACAAA+AGG + chr7_3:83720657-83720676 Msa1131190:CDS 40.0%
! TCCTTTTGTTCTCTTCAGTG+TGG - chr7_3:83720661-83720680 None:intergenic 40.0%
ACACTGAAGAGAACAAAAGG+AGG + chr7_3:83720660-83720679 Msa1131190:CDS 40.0%
ATATTGCTGCTGCAATCACT+AGG + chr7_3:83720699-83720718 Msa1131190:CDS 40.0%
ATGCTGAGATGTGGATGTTA+AGG + chr7_3:83721007-83721026 Msa1131190:three_prime_UTR 40.0%
CTGAAATTGCCTTCATTTCC+TGG + chr7_3:83721254-83721273 Msa1131190:intron 40.0%
ATCAGAAACTGTAGCTGAGA+TGG + chr7_3:83721333-83721352 Msa1131190:three_prime_UTR 40.0%
TGCAGACTTCTATTGCCTAT+TGG + chr7_3:83721490-83721509 Msa1131190:three_prime_UTR 40.0%
GCAGACTTCTATTGCCTATT+GGG + chr7_3:83721491-83721510 Msa1131190:three_prime_UTR 40.0%
ATCGAAAACGACGTAAGCTC+AGG - chr7_3:83719963-83719982 None:intergenic 45.0%
TTTCGATCATGACACACGGT+TGG + chr7_3:83719976-83719995 Msa1131190:intron 45.0%
TTCGATCATGACACACGGTT+GGG + chr7_3:83719977-83719996 Msa1131190:intron 45.0%
CGACACAGGCTCAGAAAAAA+CGG - chr7_3:83720020-83720039 None:intergenic 45.0%
CACAACCAGAATCCGCAAAT+GGG + chr7_3:83720239-83720258 Msa1131190:CDS 45.0%
ACAACCAGAATCCGCAAATG+GGG + chr7_3:83720240-83720259 Msa1131190:CDS 45.0%
TCAGCCAAACCAAATGACTG+GGG + chr7_3:83720307-83720326 Msa1131190:CDS 45.0%
TGATGTAACAACGGATCCTG+GGG - chr7_3:83720333-83720352 None:intergenic 45.0%
CTGATGTAACAACGGATCCT+GGG - chr7_3:83720334-83720353 None:intergenic 45.0%
ACTGATGTAACAACGGATCC+TGG - chr7_3:83720335-83720354 None:intergenic 45.0%
GGATCCGTTGTTACATCAGT+TGG + chr7_3:83720335-83720354 Msa1131190:CDS 45.0%
TCCGTTGTTACATCAGTTGG+GGG + chr7_3:83720338-83720357 Msa1131190:CDS 45.0%
AACAACAGCTGCAATCCTTC+TGG + chr7_3:83720453-83720472 Msa1131190:CDS 45.0%
ATCTTCTTGATCCTTGCCAG+AGG - chr7_3:83720533-83720552 None:intergenic 45.0%
GAAGATCATGAAGGCTGATG+AGG + chr7_3:83720547-83720566 Msa1131190:CDS 45.0%
GGCCTGTGAGATGTTCATAT+TGG + chr7_3:83720613-83720632 Msa1131190:CDS 45.0%
AAGCCAGCACTTCATCTTTC+AGG - chr7_3:83720769-83720788 None:intergenic 45.0%
!! TGGAACTGCTGGTGTCATAA+TGG + chr7_3:83720874-83720893 Msa1131190:CDS 45.0%
!! GGAACTGCTGGTGTCATAAT+GGG + chr7_3:83720875-83720894 Msa1131190:CDS 45.0%
! CATAATGGGAAAGCCTGTGA+TGG + chr7_3:83720889-83720908 Msa1131190:CDS 45.0%
TACATGTTTGGGTCCATCAC+AGG - chr7_3:83720905-83720924 None:intergenic 45.0%
GCTGCTGAGCATACATGTTT+GGG - chr7_3:83720916-83720935 None:intergenic 45.0%
!! CACATTTGTGGTGCCATGTA+GGG - chr7_3:83720944-83720963 None:intergenic 45.0%
ATCTAGTGATCTGGAGATGG+TGG - chr7_3:83720989-83721008 None:intergenic 45.0%
AGCATCTAGTGATCTGGAGA+TGG - chr7_3:83720992-83721011 None:intergenic 45.0%
CATCTCAGCATCTAGTGATC+TGG - chr7_3:83720998-83721017 None:intergenic 45.0%
GATCACTAGATGCTGAGATG+TGG + chr7_3:83720998-83721017 Msa1131190:exon 45.0%
ATCTGCAAGCCAGGAAATGA+AGG - chr7_3:83721266-83721285 None:intergenic 45.0%
AGATTGCAGGGAACTGCAAT+GGG + chr7_3:83721282-83721301 Msa1131190:intron 45.0%
TGGGTGTGAAACTGTTCCAT+TGG + chr7_3:83721306-83721325 Msa1131190:three_prime_UTR 45.0%
ACAGTTTCTGATCGCACCAA+TGG - chr7_3:83721325-83721344 None:intergenic 45.0%
GGAATCATAGATTTGCAGGC+AGG - chr7_3:83721564-83721583 None:intergenic 45.0%
CCATGAGAGTTGACTGCTAA+AGG - chr7_3:83721585-83721604 None:intergenic 45.0%
CCTTTAGCAGTCAACTCTCA+TGG + chr7_3:83721582-83721601 Msa1131190:three_prime_UTR 45.0%
! CTCGCACTTTTGTCTCTCAG+AGG + chr7_3:83718964-83718983 Msa1131190:five_prime_UTR 50.0%
TGTGTGAAGCTGACCTGTGA+TGG + chr7_3:83719511-83719530 Msa1131190:intron 50.0%
TGTGCATGCCTTGTGTGAAC+AGG + chr7_3:83719719-83719738 Msa1131190:intron 50.0%
TCGATCATGACACACGGTTG+GGG + chr7_3:83719978-83719997 Msa1131190:intron 50.0%
GTCGTGATCGAAAACGACAC+AGG - chr7_3:83720034-83720053 None:intergenic 50.0%
GCACAACCAGAATCCGCAAA+TGG + chr7_3:83720238-83720257 Msa1131190:CDS 50.0%
CAACCAGAATCCGCAAATGG+GGG + chr7_3:83720241-83720260 Msa1131190:CDS 50.0%
AATCCGCAAATGGGGGTTGT+CGG + chr7_3:83720248-83720267 Msa1131190:CDS 50.0%
GCCCCCAACTGATGTAACAA+CGG - chr7_3:83720342-83720361 None:intergenic 50.0%
! GTTGCTGCTGATGGATATGC+TGG - chr7_3:83720421-83720440 None:intergenic 50.0%
!! CTGGTACTGGTTTGACCAGA+AGG - chr7_3:83720471-83720490 None:intergenic 50.0%
CTTCTGGTCAAACCAGTACC+AGG + chr7_3:83720469-83720488 Msa1131190:CDS 50.0%
TCTTGATCCTTGCCAGAGGA+AGG - chr7_3:83720529-83720548 None:intergenic 50.0%
CAGGCCCTTGCAAATATGAC+AGG - chr7_3:83720599-83720618 None:intergenic 50.0%
! TCATCTTTCAGGTCCTCACG+AGG - chr7_3:83720758-83720777 None:intergenic 50.0%
GGACCTGAAAGATGAAGTGC+TGG + chr7_3:83720763-83720782 Msa1131190:CDS 50.0%
!! GTGCTGGCTTCAATTCCAAG+AGG + chr7_3:83720779-83720798 Msa1131190:CDS 50.0%
! GCAACAGGCATTGTTCCTCT+TGG - chr7_3:83720797-83720816 None:intergenic 50.0%
AGAGGAACAATGCCTGTTGC+TGG + chr7_3:83720797-83720816 Msa1131190:CDS 50.0%
!! CAGTAAGGAAGAGCATCAGC+AGG - chr7_3:83720824-83720843 None:intergenic 50.0%
TGAGGTGGCATGTAGCAGTA+AGG - chr7_3:83720839-83720858 None:intergenic 50.0%
GACACCAGCAGTTCCAACTT+GGG - chr7_3:83720870-83720889 None:intergenic 50.0%
TGACACCAGCAGTTCCAACT+TGG - chr7_3:83720871-83720890 None:intergenic 50.0%
GGCTGCTGAGCATACATGTT+TGG - chr7_3:83720917-83720936 None:intergenic 50.0%
!! TTGTGGTGCCATGTAGGGAT+GGG - chr7_3:83720939-83720958 None:intergenic 50.0%
!! TTTGTGGTGCCATGTAGGGA+TGG - chr7_3:83720940-83720959 None:intergenic 50.0%
!! CCACATTTGTGGTGCCATGT+AGG - chr7_3:83720945-83720964 None:intergenic 50.0%
CCTACATGGCACCACAAATG+TGG + chr7_3:83720942-83720961 Msa1131190:CDS 50.0%
! TTTCCTGGCTTGCAGATTGC+AGG + chr7_3:83721269-83721288 Msa1131190:intron 50.0%
! TTCCTGGCTTGCAGATTGCA+GGG + chr7_3:83721270-83721289 Msa1131190:intron 50.0%
CAGATTGCAGGGAACTGCAA+TGG + chr7_3:83721281-83721300 Msa1131190:intron 50.0%
GATTGCAGGGAACTGCAATG+GGG + chr7_3:83721283-83721302 Msa1131190:intron 50.0%
CGATCATGACACACGGTTGG+GGG + chr7_3:83719979-83719998 Msa1131190:intron 55.0%
!! GTTGGGGGCACTGCAATAGA+GGG + chr7_3:83719994-83720013 Msa1131190:intron 55.0%
CAACCCCCATTTGCGGATTC+TGG - chr7_3:83720247-83720266 None:intergenic 55.0%
CTACCGACAACCCCCATTTG+CGG - chr7_3:83720254-83720273 None:intergenic 55.0%
CAAATGGGGGTTGTCGGTAG+CGG + chr7_3:83720254-83720273 Msa1131190:CDS 55.0%
AGCGGCAGTCAAATGCCGTA+CGG + chr7_3:83720272-83720291 Msa1131190:CDS 55.0%
GTTGCTGCTGTTGCTGCTGA+TGG - chr7_3:83720430-83720449 None:intergenic 55.0%
CAAGAACCACAGCCTTCCTC+TGG + chr7_3:83720514-83720533 Msa1131190:CDS 55.0%
TGGAGTTAACCCTGCGCTCA+TGG + chr7_3:83720633-83720652 Msa1131190:CDS 55.0%
AGTGTGGTTCCATGAGCGCA+GGG - chr7_3:83720645-83720664 None:intergenic 55.0%
CACGAGGCACAATGTCAACC+AGG - chr7_3:83720742-83720761 None:intergenic 55.0%
GGTTGACATTGTGCCTCGTG+AGG + chr7_3:83720742-83720761 Msa1131190:CDS 55.0%
ACCAGCAGTTCCAACTTGGG+AGG - chr7_3:83720867-83720886 None:intergenic 55.0%
!! TGTGGTGCCATGTAGGGATG+GGG - chr7_3:83720938-83720957 None:intergenic 55.0%
ACAAATGTGGCCACAGCCTC+CGG + chr7_3:83720955-83720974 Msa1131190:CDS 55.0%
TTCCCTGCAATCTGCAAGCC+AGG - chr7_3:83721275-83721294 None:intergenic 55.0%
!! GGTTGGGGGCACTGCAATAG+AGG + chr7_3:83719993-83720012 Msa1131190:intron 60.0%
GGGGCCCCAGTCATTTGGTT+TGG - chr7_3:83720314-83720333 None:intergenic 60.0%
AACCAAATGACTGGGGCCCC+AGG + chr7_3:83720314-83720333 Msa1131190:CDS 60.0%
ATCCTGGGGCCCCAGTCATT+TGG - chr7_3:83720319-83720338 None:intergenic 60.0%
CAGTCCACTGGTCAACCTGC+TGG + chr7_3:83720365-83720384 Msa1131190:CDS 60.0%
! AGCTCCAGCAGGTTGACCAG+TGG - chr7_3:83720372-83720391 None:intergenic 60.0%
TCAACCTGCTGGAGCTCAGC+TGG + chr7_3:83720376-83720395 Msa1131190:CDS 60.0%
CAACCTGCTGGAGCTCAGCT+GGG + chr7_3:83720377-83720396 Msa1131190:CDS 60.0%
ACCACAGCCTTCCTCTGGCA+AGG + chr7_3:83720519-83720538 Msa1131190:CDS 60.0%
TCCTTGCCAGAGGAAGGCTG+TGG - chr7_3:83720523-83720542 None:intergenic 60.0%
CAGTGTGGTTCCATGAGCGC+AGG - chr7_3:83720646-83720665 None:intergenic 60.0%
GCATCAGCAGGACCAGCAAC+AGG - chr7_3:83720812-83720831 None:intergenic 60.0%
! ACTTGGGAGGCATGCTGAGG+TGG - chr7_3:83720854-83720873 None:intergenic 60.0%
! CCAACTTGGGAGGCATGCTG+AGG - chr7_3:83720857-83720876 None:intergenic 60.0%
CCTCAGCATGCCTCCCAAGT+TGG + chr7_3:83720854-83720873 Msa1131190:CDS 60.0%
! GCCTCCCAAGTTGGAACTGC+TGG + chr7_3:83720863-83720882 Msa1131190:CDS 60.0%
TCAGCAGCCCCATCCCTACA+TGG + chr7_3:83720928-83720947 Msa1131190:CDS 60.0%
GGAGGCTGTGGCCACATTTG+TGG - chr7_3:83720956-83720975 None:intergenic 60.0%
! GGAGATGGTGGTCGCTGTTC+CGG - chr7_3:83720977-83720996 None:intergenic 60.0%
TGCAGGGAACTGCAATGGGG+TGG + chr7_3:83721286-83721305 Msa1131190:three_prime_UTR 60.0%
GCAGGGAACTGCAATGGGGT+GGG + chr7_3:83721287-83721306 Msa1131190:three_prime_UTR 60.0%
! GTTGGGGGCATGCAGTCCAC+TGG + chr7_3:83720353-83720372 Msa1131190:CDS 65.0%
TGACCCAGCTGAGCTCCAGC+AGG - chr7_3:83720383-83720402 None:intergenic 65.0%
! GATGGTGGTCGCTGTTCCGG+AGG - chr7_3:83720974-83720993 None:intergenic 65.0%
GGTCGCTGTTCCGGAGGCTG+TGG - chr7_3:83720968-83720987 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr7_3 gene 83718934 83721619 83718934 ID=Msa1131190;Name=Msa1131190
chr7_3 mRNA 83718934 83721619 83718934 ID=Msa1131190-mRNA-1;Parent=Msa1131190;Name=Msa1131190-mRNA-1;_AED=0.01;_eAED=0.01;_QI=96|1|1|1|0|0|4|358|260
chr7_3 exon 83718934 83718985 83718934 ID=Msa1131190-mRNA-1:exon:24830;Parent=Msa1131190-mRNA-1
chr7_3 exon 83719264 83719306 83719264 ID=Msa1131190-mRNA-1:exon:24831;Parent=Msa1131190-mRNA-1
chr7_3 exon 83720223 83721028 83720223 ID=Msa1131190-mRNA-1:exon:24832;Parent=Msa1131190-mRNA-1
chr7_3 exon 83721284 83721619 83721284 ID=Msa1131190-mRNA-1:exon:24833;Parent=Msa1131190-mRNA-1
chr7_3 five_prime_UTR 83718934 83718985 83718934 ID=Msa1131190-mRNA-1:five_prime_utr;Parent=Msa1131190-mRNA-1
chr7_3 five_prime_UTR 83719264 83719306 83719264 ID=Msa1131190-mRNA-1:five_prime_utr;Parent=Msa1131190-mRNA-1
chr7_3 five_prime_UTR 83720223 83720223 83720223 ID=Msa1131190-mRNA-1:five_prime_utr;Parent=Msa1131190-mRNA-1
chr7_3 CDS 83720224 83721006 83720224 ID=Msa1131190-mRNA-1:cds;Parent=Msa1131190-mRNA-1
chr7_3 three_prime_UTR 83721007 83721028 83721007 ID=Msa1131190-mRNA-1:three_prime_utr;Parent=Msa1131190-mRNA-1
chr7_3 three_prime_UTR 83721284 83721619 83721284 ID=Msa1131190-mRNA-1:three_prime_utr;Parent=Msa1131190-mRNA-1
Gene Sequence

>Msa1131190

ATGGATCATCAAGGGCACAACCAGAATCCGCAAATGGGGGTTGTCGGTAGCGGCAGTCAAATGCCGTACGGATCTAACCCATATCAGCCAAACCAAATGACTGGGGCCCCAGGATCCGTTGTTACATCAGTTGGGGGCATGCAGTCCACTGGTCAACCTGCTGGAGCTCAGCTGGGTCAGCATCAACTTGCTTACCAGCATATCCATCAGCAGCAACAGCAGCAACTTCAACAACAGCTGCAATCCTTCTGGTCAAACCAGTACCAGGAAATTGAAAAGGTTACTGATTTCAAGAACCACAGCCTTCCTCTGGCAAGGATCAAGAAGATCATGAAGGCTGATGAGGATGTTAGAATGATATCAGCTGAAGCACCTGTCATATTTGCAAGGGCCTGTGAGATGTTCATATTGGAGTTAACCCTGCGCTCATGGAACCACACTGAAGAGAACAAAAGGAGGACACTTCAGAAGAATGATATTGCTGCTGCAATCACTAGGACTGATATCTTTGATTTCCTGGTTGACATTGTGCCTCGTGAGGACCTGAAAGATGAAGTGCTGGCTTCAATTCCAAGAGGAACAATGCCTGTTGCTGGTCCTGCTGATGCTCTTCCTTACTGCTACATGCCACCTCAGCATGCCTCCCAAGTTGGAACTGCTGGTGTCATAATGGGAAAGCCTGTGATGGACCCAAACATGTATGCTCAGCAGCCCCATCCCTACATGGCACCACAAATGTGGCCACAGCCTCCGGAACAGCGACCACCATCTCCAGATCACTAG
Protein sequence

>Msa1131190

MDHQGHNQNPQMGVVGSGSQMPYGSNPYQPNQMTGAPGSVVTSVGGMQSTGQPAGAQLGQHQLAYQHIHQQQQQQLQQQLQSFWSNQYQEIEKVTDFKNHSLPLARIKKIMKADEDVRMISAEAPVIFARACEMFILELTLRSWNHTEENKRRTLQKNDIAAAITRTDIFDFLVDIVPREDLKDEVLASIPRGTMPVAGPADALPYCYMPPQHASQVGTAGVIMGKPVMDPNMYAQQPHPYMAPQMWPQPPEQRPPSPDH*