AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1381220

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1381220 MtrunA17_Chr7g0272991 99.615 260 1 0 1 260 1 260 0.0 537
Msa1381220 MtrunA17_Chr1g0191681 83.539 243 36 1 1 243 1 239 3.62e-140 394
Msa1381220 MtrunA17_Chr2g0310741 55.785 242 92 7 12 247 15 247 9.87e-76 231
Msa1381220 MtrunA17_Chr3g0132191 66.038 159 39 4 83 238 37 183 1.24e-62 196
Msa1381220 MtrunA17_Chr2g0288481 49.468 188 70 8 82 259 66 238 6.82e-53 172
Msa1381220 MtrunA17_Chr2g0318881 59.770 87 34 1 100 185 21 107 1.64e-31 112
Msa1381220 MtrunA17_Chr2g0318921 61.446 83 31 1 100 181 21 103 6.70e-31 111
Msa1381220 MtrunA17_Chr8g0376351 61.333 75 28 1 119 192 1 75 2.16e-27 101
Msa1381220 MtrunA17_Chr3g0081161 39.744 78 47 0 102 179 9 86 7.89e-12 63.9
Msa1381220 MtrunA17_Chr8g0354151 37.179 78 49 0 102 179 9 86 1.47e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1381220 AT1G08970.3 62.500 248 63 8 1 247 1 219 4.92e-88 261
Msa1381220 AT1G08970.4 62.500 248 63 8 1 247 1 219 4.92e-88 261
Msa1381220 AT1G08970.2 62.500 248 63 8 1 247 1 219 4.92e-88 261
Msa1381220 AT1G08970.1 62.500 248 63 8 1 247 1 219 4.92e-88 261
Msa1381220 AT1G54830.2 63.636 242 56 5 16 256 5 215 2.92e-87 259
Msa1381220 AT1G54830.3 63.636 242 56 5 16 256 5 215 2.92e-87 259
Msa1381220 AT1G54830.1 63.636 242 56 5 16 256 5 215 2.92e-87 259
Msa1381220 AT3G48590.1 68.790 157 44 3 83 238 47 199 1.76e-70 216
Msa1381220 AT5G63470.2 68.987 158 45 4 83 238 60 215 2.07e-67 209
Msa1381220 AT5G63470.1 68.987 158 45 4 83 238 60 215 2.07e-67 209
Msa1381220 AT1G56170.1 75.758 132 25 3 80 209 55 181 1.35e-66 205
Msa1381220 AT1G56170.2 75.758 132 25 3 80 209 55 181 1.35e-66 205
Msa1381220 AT5G50480.1 49.123 171 63 5 79 248 35 182 2.74e-46 154
Msa1381220 AT5G27910.1 45.349 172 72 8 77 248 15 164 4.68e-39 134
Msa1381220 AT5G50490.1 38.889 180 96 4 70 249 8 173 1.71e-34 123
Msa1381220 AT5G50470.1 39.560 182 71 8 79 251 44 195 9.78e-31 114
Msa1381220 AT5G38140.1 43.269 104 55 2 76 178 44 144 1.41e-25 100
Msa1381220 AT3G12480.1 37.179 78 49 0 102 179 9 86 1.30e-11 63.9

Find 123 sgRNAs with CRISPR-Local

Find 193 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGCTGGGTAAAATGTAATT+TGG 0.125571 tig0020424:+29058 Msa1381220:three_prime_UTR
AAGCCAGCACTTCATCTTTC+AGG 0.158704 tig0020424:-28402 None:intergenic
GTCATTTGGTTTGGCTGATA+TGG 0.158964 tig0020424:-27938 None:intergenic
GACTGATATCTTTGATTTCC+TGG 0.246183 tig0020424:+28357 Msa1381220:CDS
TAATTTGGGTTTAAACCTTA+TGG 0.256597 tig0020424:+29073 Msa1381220:three_prime_UTR
TCAACCTGCTGGAGCTCAGC+TGG 0.263146 tig0020424:+28012 Msa1381220:CDS
GCAGACTTCTATTGCCTATT+GGG 0.267661 tig0020424:+29128 Msa1381220:three_prime_UTR
TGCAGACTTCTATTGCCTAT+TGG 0.280582 tig0020424:+29127 Msa1381220:three_prime_UTR
ATCTTTATGTTTAATTAAGC+TGG 0.286907 tig0020424:+29042 Msa1381220:three_prime_UTR
CTATTGGGTTATATTTACTA+TGG 0.312585 tig0020424:+29143 Msa1381220:three_prime_UTR
GGGGCCCCAGTCATTTGGTT+TGG 0.316348 tig0020424:-27947 None:intergenic
AGCTGGGTAAAATGTAATTT+GGG 0.319298 tig0020424:+29059 Msa1381220:three_prime_UTR
TTTCCTGGCTTGCAGATTGC+AGG 0.326598 tig0020424:+28905 Msa1381220:intron
GGAGATGGTGGTCGCTGTTC+CGG 0.343092 tig0020424:-28610 None:intergenic
TAAAGGAATCATAGATTTGC+AGG 0.344712 tig0020424:-29202 None:intergenic
GGCTGCTGAGCATACATGTT+TGG 0.345011 tig0020424:-28550 None:intergenic
GCCTCCCAAGTTGGAACTGC+TGG 0.356093 tig0020424:+28499 Msa1381220:CDS
ACATAAAGATAAGTTATTAC+TGG 0.361320 tig0020424:-29029 None:intergenic
GATCCGTTGTTACATCAGTT+GGG 0.366704 tig0020424:+27972 Msa1381220:CDS
ATCCTGGGGCCCCAGTCATT+TGG 0.379289 tig0020424:-27952 None:intergenic
TCATTTGGTTTGGCTGATAT+GGG 0.380613 tig0020424:-27937 None:intergenic
GGTTTAAACCTTATGGTCTT+CGG 0.383720 tig0020424:+29080 Msa1381220:three_prime_UTR
ACTGATGTAACAACGGATCC+TGG 0.389880 tig0020424:-27968 None:intergenic
TGGAACTGCTGGTGTCATAA+TGG 0.411463 tig0020424:+28510 Msa1381220:CDS
CACAACCAGAATCCGCAAAT+GGG 0.411828 tig0020424:+27875 Msa1381220:CDS
AGAGGAACAATGCCTGTTGC+TGG 0.417994 tig0020424:+28433 Msa1381220:CDS
GGATCCGTTGTTACATCAGT+TGG 0.426256 tig0020424:+27971 Msa1381220:CDS
CATAGTAAATATAACCCAAT+AGG 0.426321 tig0020424:-29142 None:intergenic
AACAACAGCTGCAATCCTTC+TGG 0.435091 tig0020424:+28089 Msa1381220:CDS
GCTGCTGAGCATACATGTTT+GGG 0.435445 tig0020424:-28549 None:intergenic
CATCTCAGCATCTAGTGATC+TGG 0.437549 tig0020424:-28631 None:intergenic
TCATGGTCTTCTACCCCATC+GGG 0.441080 tig0020424:+29236 Msa1381220:three_prime_UTR
AGATTGCAGGGAACTGCAAT+GGG 0.446408 tig0020424:+28918 Msa1381220:intron
CAGTGTGGTTCCATGAGCGC+AGG 0.449372 tig0020424:-28279 None:intergenic
ACAAATGTGGCCACAGCCTC+CGG 0.450073 tig0020424:+28591 Msa1381220:CDS
GCACAACCAGAATCCGCAAA+TGG 0.451270 tig0020424:+27874 Msa1381220:CDS
CAAGAACCACAGCCTTCCTC+TGG 0.452736 tig0020424:+28150 Msa1381220:CDS
GCATCAGCAGGACCAGCAAC+AGG 0.455014 tig0020424:-28445 None:intergenic
GTTGCTGCTGTTGCTGCTGA+TGG 0.455992 tig0020424:-28063 None:intergenic
GGCCTGTGAGATGTTCATAT+TGG 0.456864 tig0020424:+28249 Msa1381220:CDS
CCATGAGAGTTGACTGCTAA+AGG 0.458260 tig0020424:-29219 None:intergenic
TGGAGTTAACCCTGCGCTCA+TGG 0.458619 tig0020424:+28269 Msa1381220:CDS
CAGTCCACTGGTCAACCTGC+TGG 0.464075 tig0020424:+28001 Msa1381220:CDS
CACATTTGTGGTGCCATGTA+GGG 0.465255 tig0020424:-28577 None:intergenic
CTGATGTAACAACGGATCCT+GGG 0.469388 tig0020424:-27967 None:intergenic
CTGGTACTGGTTTGACCAGA+AGG 0.471679 tig0020424:-28104 None:intergenic
CAACCTGCTGGAGCTCAGCT+GGG 0.476224 tig0020424:+28013 Msa1381220:CDS
ATGCTGAGATGTGGATGTTA+AGG 0.476601 tig0020424:+28643 Msa1381220:three_prime_UTR
TGACACCAGCAGTTCCAACT+TGG 0.478083 tig0020424:-28504 None:intergenic
GGAACTGCTGGTGTCATAAT+GGG 0.479046 tig0020424:+28511 Msa1381220:CDS
TCTTTATGTTTAATTAAGCT+GGG 0.482086 tig0020424:+29043 Msa1381220:three_prime_UTR
TTGTGGTGCCATGTAGGGAT+GGG 0.484954 tig0020424:-28572 None:intergenic
AACCAAATGACTGGGGCCCC+AGG 0.486456 tig0020424:+27950 Msa1381220:CDS
TTTGTGGTGCCATGTAGGGA+TGG 0.486986 tig0020424:-28573 None:intergenic
GGAGGCTGTGGCCACATTTG+TGG 0.486993 tig0020424:-28589 None:intergenic
GCAACAGGCATTGTTCCTCT+TGG 0.487666 tig0020424:-28430 None:intergenic
TGGGTGTGAAACTGTTCCAT+TGG 0.491473 tig0020424:+28943 Msa1381220:three_prime_UTR
ACCACACTGAAGAGAACAAA+AGG 0.492883 tig0020424:+28293 Msa1381220:CDS
CCACATTTGTGGTGCCATGT+AGG 0.497501 tig0020424:-28578 None:intergenic
TACATGTTTGGGTCCATCAC+AGG 0.504424 tig0020424:-28538 None:intergenic
TATATCATCCGAAGACCATA+AGG 0.504471 tig0020424:-29088 None:intergenic
TATCAGCCAAACCAAATGAC+TGG 0.504884 tig0020424:+27941 Msa1381220:CDS
CCTCAGCATGCCTCCCAAGT+TGG 0.514710 tig0020424:+28490 Msa1381220:CDS
TCTTGATCCTTGCCAGAGGA+AGG 0.515769 tig0020424:-28162 None:intergenic
GTTGCTGCTGATGGATATGC+TGG 0.517658 tig0020424:-28054 None:intergenic
TGACCCAGCTGAGCTCCAGC+AGG 0.517937 tig0020424:-28016 None:intergenic
CCAGTACCAGGAAATTGAAA+AGG 0.518655 tig0020424:+28117 Msa1381220:CDS
CTTCTGGTCAAACCAGTACC+AGG 0.521956 tig0020424:+28105 Msa1381220:CDS
TTCCTGGCTTGCAGATTGCA+GGG 0.525676 tig0020424:+28906 Msa1381220:intron
CCTTTAGCAGTCAACTCTCA+TGG 0.529370 tig0020424:+29219 Msa1381220:three_prime_UTR
CAGATTGCAGGGAACTGCAA+TGG 0.530413 tig0020424:+28917 Msa1381220:intron
ATCCGTTGTTACATCAGTTG+GGG 0.531831 tig0020424:+27973 Msa1381220:CDS
CTCATGGTCTTCTACCCCAT+CGG 0.534855 tig0020424:+29235 Msa1381220:three_prime_UTR
GGTCGCTGTTCCGGAGGCTG+TGG 0.539229 tig0020424:-28601 None:intergenic
GGACCTGAAAGATGAAGTGC+TGG 0.542953 tig0020424:+28399 Msa1381220:CDS
TGTGGTGCCATGTAGGGATG+GGG 0.546062 tig0020424:-28571 None:intergenic
ACTTGGGAGGCATGCTGAGG+TGG 0.548128 tig0020424:-28487 None:intergenic
ATCAGCCAAACCAAATGACT+GGG 0.550264 tig0020424:+27942 Msa1381220:CDS
GACACCAGCAGTTCCAACTT+GGG 0.552393 tig0020424:-28503 None:intergenic
AAGCACCTGTCATATTTGCA+AGG 0.553287 tig0020424:+28227 Msa1381220:CDS
CAGTAAGGAAGAGCATCAGC+AGG 0.560205 tig0020424:-28457 None:intergenic
CAGGCCCTTGCAAATATGAC+AGG 0.560856 tig0020424:-28232 None:intergenic
TCAGCAGCCCCATCCCTACA+TGG 0.562305 tig0020424:+28564 Msa1381220:CDS
ATCAGAAACTGTAGCTGAGA+TGG 0.564284 tig0020424:+28970 Msa1381220:three_prime_UTR
TCCTTGCCAGAGGAAGGCTG+TGG 0.564697 tig0020424:-28156 None:intergenic
GGAATCATAGATTTGCAGGC+AGG 0.566600 tig0020424:-29198 None:intergenic
CAAAACAGAATGGATCATCA+AGG 0.570283 tig0020424:+27851 Msa1381220:intron
AGCATCTAGTGATCTGGAGA+TGG 0.574668 tig0020424:-28625 None:intergenic
ATATTGCTGCTGCAATCACT+AGG 0.575052 tig0020424:+28335 Msa1381220:CDS
GAAGATCATGAAGGCTGATG+AGG 0.575398 tig0020424:+28183 Msa1381220:CDS
TGAGGTGGCATGTAGCAGTA+AGG 0.584291 tig0020424:-28472 None:intergenic
ACCACAGCCTTCCTCTGGCA+AGG 0.585880 tig0020424:+28155 Msa1381220:CDS
ACAGTTTCTGATCGCACCAA+TGG 0.588674 tig0020424:-28959 None:intergenic
AGCTCCAGCAGGTTGACCAG+TGG 0.593284 tig0020424:-28005 None:intergenic
CATAATGGGAAAGCCTGTGA+TGG 0.593370 tig0020424:+28525 Msa1381220:CDS
CACGAGGCACAATGTCAACC+AGG 0.593690 tig0020424:-28375 None:intergenic
ACAACCAGAATCCGCAAATG+GGG 0.594889 tig0020424:+27876 Msa1381220:CDS
ATTGCAGGGAACTGCAATGG+GGG 0.605277 tig0020424:+28920 Msa1381220:three_prime_UTR
ATCTAGTGATCTGGAGATGG+TGG 0.605869 tig0020424:-28622 None:intergenic
CTCCAATATGAACATCTCAC+AGG 0.609517 tig0020424:-28251 None:intergenic
TGATATGGGTTAGATCCGTA+CGG 0.617785 tig0020424:-27923 None:intergenic
AGCGGCAGTCAAATGCCGTA+CGG 0.619913 tig0020424:+27908 Msa1381220:CDS
TGATGTAACAACGGATCCTG+GGG 0.620797 tig0020424:-27966 None:intergenic
AGCACCTGTCATATTTGCAA+GGG 0.622434 tig0020424:+28228 Msa1381220:CDS
ATCTTCTTGATCCTTGCCAG+AGG 0.624577 tig0020424:-28166 None:intergenic
AAAACAGAATGGATCATCAA+GGG 0.625548 tig0020424:+27852 Msa1381220:intron
ACCAGCAGTTCCAACTTGGG+AGG 0.637100 tig0020424:-28500 None:intergenic
AGTGTGGTTCCATGAGCGCA+GGG 0.637592 tig0020424:-28278 None:intergenic
GATCACTAGATGCTGAGATG+TGG 0.638929 tig0020424:+28634 Msa1381220:exon
CCTACATGGCACCACAAATG+TGG 0.644640 tig0020424:+28578 Msa1381220:CDS
GATGGTGGTCGCTGTTCCGG+AGG 0.649506 tig0020424:-28607 None:intergenic
CCAACTTGGGAGGCATGCTG+AGG 0.657795 tig0020424:-28490 None:intergenic
ATTCCTTCTATTCCCCGATG+GGG 0.660173 tig0020424:-29249 None:intergenic
TCATCTTTCAGGTCCTCACG+AGG 0.665344 tig0020424:-28391 None:intergenic
AAGGATCAAGAAGATCATGA+AGG 0.667741 tig0020424:+28174 Msa1381220:CDS
TCCGTTGTTACATCAGTTGG+GGG 0.670764 tig0020424:+27974 Msa1381220:CDS
GGTTGACATTGTGCCTCGTG+AGG 0.672349 tig0020424:+28378 Msa1381220:CDS
CAACCAGAATCCGCAAATGG+GGG 0.676007 tig0020424:+27877 Msa1381220:CDS
ACACTGAAGAGAACAAAAGG+AGG 0.682027 tig0020424:+28296 Msa1381220:CDS
GATTGCAGGGAACTGCAATG+GGG 0.700328 tig0020424:+28919 Msa1381220:intron
CATGGTCTTCTACCCCATCG+GGG 0.703842 tig0020424:+29237 Msa1381220:three_prime_UTR
GTGCTGGCTTCAATTCCAAG+AGG 0.714242 tig0020424:+28415 Msa1381220:CDS
TCAGCCAAACCAAATGACTG+GGG 0.740826 tig0020424:+27943 Msa1381220:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGATTATTGTTTTTCTTTTT+TGG + tig0020424:27093-27112 Msa1381220:intron 15.0%
!! CAAAAAAAAAACATCAAAAA+AGG - tig0020424:27225-27244 None:intergenic 15.0%
!!! TGTAAATTGAAAACTTATTT+TGG + tig0020424:27414-27433 Msa1381220:intron 15.0%
!!! TTTCATTTTCAAAGAAAAGA+AGG + tig0020424:26640-26659 Msa1381220:intron 20.0%
!! AAGAAGAGAAAAAAAGTTTA+GGG - tig0020424:26742-26761 None:intergenic 20.0%
!! AAAGAAGAGAAAAAAAGTTT+AGG - tig0020424:26743-26762 None:intergenic 20.0%
!!! TAAACTTTTTTTCTCTTCTT+TGG + tig0020424:26742-26761 Msa1381220:intron 20.0%
!!! AAACTTTTTTTCTCTTCTTT+GGG + tig0020424:26743-26762 Msa1381220:intron 20.0%
!! ATACAGAAAAAAAAATGAAC+TGG - tig0020424:26965-26984 None:intergenic 20.0%
!! TCAACAGAACAAAAATTATT+CGG - tig0020424:27300-27319 None:intergenic 20.0%
!!! AAACTTATTTTGGTATTCTA+AGG + tig0020424:27424-27443 Msa1381220:intron 20.0%
!!! AACTTATTTTGGTATTCTAA+GGG + tig0020424:27425-27444 Msa1381220:intron 20.0%
!! CTAATAAAATAGCATACAAA+AGG - tig0020424:28766-28785 None:intergenic 20.0%
!!! TTTTATTAGTTAGCTTGATT+AGG + tig0020424:28777-28796 Msa1381220:intron 20.0%
!! ACATAAAGATAAGTTATTAC+TGG - tig0020424:29032-29051 None:intergenic 20.0%
!! ATCTTTATGTTTAATTAAGC+TGG + tig0020424:29042-29061 Msa1381220:three_prime_UTR 20.0%
!! TCTTTATGTTTAATTAAGCT+GGG + tig0020424:29043-29062 Msa1381220:three_prime_UTR 20.0%
!!! TCATTTTTTGTTTCTCTGAA+TGG + tig0020424:27253-27272 Msa1381220:intron 25.0%
!! GATTTTATGTCAAAACAGAA+TGG + tig0020424:27841-27860 Msa1381220:intron 25.0%
!! TAATTTGGGTTTAAACCTTA+TGG + tig0020424:29073-29092 Msa1381220:three_prime_UTR 25.0%
! CATAGTAAATATAACCCAAT+AGG - tig0020424:29145-29164 None:intergenic 25.0%
! CTATTGGGTTATATTTACTA+TGG + tig0020424:29143-29162 Msa1381220:three_prime_UTR 25.0%
! GAGTTGTTATGATAATGCTT+TGG - tig0020424:26782-26801 None:intergenic 30.0%
AAAACTGAGAACCTGAAAAA+GGG - tig0020424:26893-26912 None:intergenic 30.0%
GAATGGAGAATACATATTAC+TGG + tig0020424:27270-27289 Msa1381220:intron 30.0%
!! GAAGATTTGTTGATCAAATC+TGG + tig0020424:27447-27466 Msa1381220:intron 30.0%
GATTTATGCATGTCATGAAA+TGG + tig0020424:27554-27573 Msa1381220:intron 30.0%
GTTTAATGCATAGGAAAAAC+GGG - tig0020424:27706-27725 None:intergenic 30.0%
CGTTTAATGCATAGGAAAAA+CGG - tig0020424:27707-27726 None:intergenic 30.0%
! CGTTTTTCCTATGCATTAAA+CGG + tig0020424:27705-27724 Msa1381220:intron 30.0%
AACAAATCATGCAATCATTG+AGG - tig0020424:27783-27802 None:intergenic 30.0%
CATAAAATCAAACACGTGAT+CGG - tig0020424:27830-27849 None:intergenic 30.0%
AAAACAGAATGGATCATCAA+GGG + tig0020424:27852-27871 Msa1381220:intron 30.0%
TCCTTATACTTGATCTTTGA+TGG + tig0020424:28828-28847 Msa1381220:intron 30.0%
ACCATCAAAGATCAAGTATA+AGG - tig0020424:28832-28851 None:intergenic 30.0%
! CGCACATTTACTGTTATTTA+TGG + tig0020424:28852-28871 Msa1381220:intron 30.0%
AAGGCAATTTCAGACATAAA+AGG - tig0020424:28883-28902 None:intergenic 30.0%
AAGCTGGGTAAAATGTAATT+TGG + tig0020424:29058-29077 Msa1381220:three_prime_UTR 30.0%
AGCTGGGTAAAATGTAATTT+GGG + tig0020424:29059-29078 Msa1381220:three_prime_UTR 30.0%
TAAAGGAATCATAGATTTGC+AGG - tig0020424:29205-29224 None:intergenic 30.0%
!! GATTGATGATGGTGTTTGTT+TGG - tig0020424:26682-26701 None:intergenic 35.0%
GTTGTTGTGAAGATTGATGA+TGG - tig0020424:26693-26712 None:intergenic 35.0%
!! TCTTGTTTGATCCCTTTTTC+AGG + tig0020424:26879-26898 Msa1381220:intron 35.0%
GAAAACTGAGAACCTGAAAA+AGG - tig0020424:26894-26913 None:intergenic 35.0%
!!! AACCAAGCTTCGTTTTTTGA+GGG + tig0020424:26918-26937 Msa1381220:five_prime_UTR 35.0%
TTCATAGTTATGCTGTAACG+TGG + tig0020424:27018-27037 Msa1381220:intron 35.0%
AAATTCTTAGCTTCCATCAC+AGG - tig0020424:27164-27183 None:intergenic 35.0%
TTCGAGATACAATTGTGCAT+GGG + tig0020424:27502-27521 Msa1381220:intron 35.0%
AAGCTCAGGAGAAAAATAAC+AGG - tig0020424:27585-27604 None:intergenic 35.0%
CAACATTTCACAAACGACAA+AGG - tig0020424:27807-27826 None:intergenic 35.0%
CAAAACAGAATGGATCATCA+AGG + tig0020424:27851-27870 Msa1381220:intron 35.0%
!! TCATTTGGTTTGGCTGATAT+GGG - tig0020424:27940-27959 None:intergenic 35.0%
! CAGTAACCTTTTCAATTTCC+TGG - tig0020424:28126-28145 None:intergenic 35.0%
AAGGATCAAGAAGATCATGA+AGG + tig0020424:28174-28193 Msa1381220:CDS 35.0%
! GACTGATATCTTTGATTTCC+TGG + tig0020424:28357-28376 Msa1381220:CDS 35.0%
GGTTTAAACCTTATGGTCTT+CGG + tig0020424:29080-29099 Msa1381220:three_prime_UTR 35.0%
TATATCATCCGAAGACCATA+AGG - tig0020424:29091-29110 None:intergenic 35.0%
AATCGTGAGAAACGAGAGAA+TGG - tig0020424:26858-26877 None:intergenic 40.0%
!!! GAACCAAGCTTCGTTTTTTG+AGG + tig0020424:26917-26936 Msa1381220:five_prime_UTR 40.0%
TTCCCTCAAAAAACGAAGCT+TGG - tig0020424:26923-26942 None:intergenic 40.0%
!!! AAGCTTCGTTTTTTGAGGGA+AGG + tig0020424:26922-26941 Msa1381220:five_prime_UTR 40.0%
CTAAAAGACCTGTTCACACA+AGG - tig0020424:27366-27385 None:intergenic 40.0%
GTTCGAGATACAATTGTGCA+TGG + tig0020424:27501-27520 Msa1381220:intron 40.0%
! TCGTTTTCGATCATGACACA+CGG + tig0020424:27608-27627 Msa1381220:intron 40.0%
AGCGTAACCGTTTAATGCAT+AGG - tig0020424:27715-27734 None:intergenic 40.0%
!! TGATATGGGTTAGATCCGTA+CGG - tig0020424:27926-27945 None:intergenic 40.0%
!! GTCATTTGGTTTGGCTGATA+TGG - tig0020424:27941-27960 None:intergenic 40.0%
TATCAGCCAAACCAAATGAC+TGG + tig0020424:27941-27960 Msa1381220:CDS 40.0%
ATCAGCCAAACCAAATGACT+GGG + tig0020424:27942-27961 Msa1381220:CDS 40.0%
GATCCGTTGTTACATCAGTT+GGG + tig0020424:27972-27991 Msa1381220:CDS 40.0%
ATCCGTTGTTACATCAGTTG+GGG + tig0020424:27973-27992 Msa1381220:CDS 40.0%
! CCTTTTCAATTTCCTGGTAC+TGG - tig0020424:28120-28139 None:intergenic 40.0%
CCAGTACCAGGAAATTGAAA+AGG + tig0020424:28117-28136 Msa1381220:CDS 40.0%
AAGCACCTGTCATATTTGCA+AGG + tig0020424:28227-28246 Msa1381220:CDS 40.0%
AGCACCTGTCATATTTGCAA+GGG + tig0020424:28228-28247 Msa1381220:CDS 40.0%
CTCCAATATGAACATCTCAC+AGG - tig0020424:28254-28273 None:intergenic 40.0%
ACCACACTGAAGAGAACAAA+AGG + tig0020424:28293-28312 Msa1381220:CDS 40.0%
! TCCTTTTGTTCTCTTCAGTG+TGG - tig0020424:28297-28316 None:intergenic 40.0%
ACACTGAAGAGAACAAAAGG+AGG + tig0020424:28296-28315 Msa1381220:CDS 40.0%
ATATTGCTGCTGCAATCACT+AGG + tig0020424:28335-28354 Msa1381220:CDS 40.0%
ATGCTGAGATGTGGATGTTA+AGG + tig0020424:28643-28662 Msa1381220:three_prime_UTR 40.0%
CTGAAATTGCCTTCATTTCC+TGG + tig0020424:28890-28909 Msa1381220:intron 40.0%
ATCAGAAACTGTAGCTGAGA+TGG + tig0020424:28970-28989 Msa1381220:three_prime_UTR 40.0%
TGCAGACTTCTATTGCCTAT+TGG + tig0020424:29127-29146 Msa1381220:three_prime_UTR 40.0%
GCAGACTTCTATTGCCTATT+GGG + tig0020424:29128-29147 Msa1381220:three_prime_UTR 40.0%
ATCGAAAACGACGTAAGCTC+AGG - tig0020424:27599-27618 None:intergenic 45.0%
TTTCGATCATGACACACGGT+TGG + tig0020424:27612-27631 Msa1381220:intron 45.0%
TTCGATCATGACACACGGTT+GGG + tig0020424:27613-27632 Msa1381220:intron 45.0%
CGACACAGGCTCAGAAAAAA+CGG - tig0020424:27656-27675 None:intergenic 45.0%
CACAACCAGAATCCGCAAAT+GGG + tig0020424:27875-27894 Msa1381220:CDS 45.0%
ACAACCAGAATCCGCAAATG+GGG + tig0020424:27876-27895 Msa1381220:CDS 45.0%
TCAGCCAAACCAAATGACTG+GGG + tig0020424:27943-27962 Msa1381220:CDS 45.0%
TGATGTAACAACGGATCCTG+GGG - tig0020424:27969-27988 None:intergenic 45.0%
CTGATGTAACAACGGATCCT+GGG - tig0020424:27970-27989 None:intergenic 45.0%
ACTGATGTAACAACGGATCC+TGG - tig0020424:27971-27990 None:intergenic 45.0%
GGATCCGTTGTTACATCAGT+TGG + tig0020424:27971-27990 Msa1381220:CDS 45.0%
TCCGTTGTTACATCAGTTGG+GGG + tig0020424:27974-27993 Msa1381220:CDS 45.0%
AACAACAGCTGCAATCCTTC+TGG + tig0020424:28089-28108 Msa1381220:CDS 45.0%
ATCTTCTTGATCCTTGCCAG+AGG - tig0020424:28169-28188 None:intergenic 45.0%
GAAGATCATGAAGGCTGATG+AGG + tig0020424:28183-28202 Msa1381220:CDS 45.0%
GGCCTGTGAGATGTTCATAT+TGG + tig0020424:28249-28268 Msa1381220:CDS 45.0%
AAGCCAGCACTTCATCTTTC+AGG - tig0020424:28405-28424 None:intergenic 45.0%
!! TGGAACTGCTGGTGTCATAA+TGG + tig0020424:28510-28529 Msa1381220:CDS 45.0%
!! GGAACTGCTGGTGTCATAAT+GGG + tig0020424:28511-28530 Msa1381220:CDS 45.0%
! CATAATGGGAAAGCCTGTGA+TGG + tig0020424:28525-28544 Msa1381220:CDS 45.0%
TACATGTTTGGGTCCATCAC+AGG - tig0020424:28541-28560 None:intergenic 45.0%
GCTGCTGAGCATACATGTTT+GGG - tig0020424:28552-28571 None:intergenic 45.0%
!! CACATTTGTGGTGCCATGTA+GGG - tig0020424:28580-28599 None:intergenic 45.0%
ATCTAGTGATCTGGAGATGG+TGG - tig0020424:28625-28644 None:intergenic 45.0%
AGCATCTAGTGATCTGGAGA+TGG - tig0020424:28628-28647 None:intergenic 45.0%
CATCTCAGCATCTAGTGATC+TGG - tig0020424:28634-28653 None:intergenic 45.0%
GATCACTAGATGCTGAGATG+TGG + tig0020424:28634-28653 Msa1381220:exon 45.0%
ATCTGCAAGCCAGGAAATGA+AGG - tig0020424:28902-28921 None:intergenic 45.0%
AGATTGCAGGGAACTGCAAT+GGG + tig0020424:28918-28937 Msa1381220:intron 45.0%
TGGGTGTGAAACTGTTCCAT+TGG + tig0020424:28943-28962 Msa1381220:three_prime_UTR 45.0%
ACAGTTTCTGATCGCACCAA+TGG - tig0020424:28962-28981 None:intergenic 45.0%
GGAATCATAGATTTGCAGGC+AGG - tig0020424:29201-29220 None:intergenic 45.0%
CCATGAGAGTTGACTGCTAA+AGG - tig0020424:29222-29241 None:intergenic 45.0%
CCTTTAGCAGTCAACTCTCA+TGG + tig0020424:29219-29238 Msa1381220:three_prime_UTR 45.0%
! CTCGCACTTTTGTCTCTCAG+AGG + tig0020424:26603-26622 Msa1381220:five_prime_UTR 50.0%
TGTGTGAAGCTGACCTGTGA+TGG + tig0020424:27148-27167 Msa1381220:intron 50.0%
TGTGCATGCCTTGTGTGAAC+AGG + tig0020424:27355-27374 Msa1381220:intron 50.0%
TCGATCATGACACACGGTTG+GGG + tig0020424:27614-27633 Msa1381220:intron 50.0%
GTCGTGATCGAAAACGACAC+AGG - tig0020424:27670-27689 None:intergenic 50.0%
GCACAACCAGAATCCGCAAA+TGG + tig0020424:27874-27893 Msa1381220:CDS 50.0%
CAACCAGAATCCGCAAATGG+GGG + tig0020424:27877-27896 Msa1381220:CDS 50.0%
AATCCGCAAATGGGGGTTGT+CGG + tig0020424:27884-27903 Msa1381220:CDS 50.0%
GCCCCCAACTGATGTAACAA+CGG - tig0020424:27978-27997 None:intergenic 50.0%
! GTTGCTGCTGATGGATATGC+TGG - tig0020424:28057-28076 None:intergenic 50.0%
!! CTGGTACTGGTTTGACCAGA+AGG - tig0020424:28107-28126 None:intergenic 50.0%
CTTCTGGTCAAACCAGTACC+AGG + tig0020424:28105-28124 Msa1381220:CDS 50.0%
TCTTGATCCTTGCCAGAGGA+AGG - tig0020424:28165-28184 None:intergenic 50.0%
CAGGCCCTTGCAAATATGAC+AGG - tig0020424:28235-28254 None:intergenic 50.0%
! TCATCTTTCAGGTCCTCACG+AGG - tig0020424:28394-28413 None:intergenic 50.0%
GGACCTGAAAGATGAAGTGC+TGG + tig0020424:28399-28418 Msa1381220:CDS 50.0%
!! GTGCTGGCTTCAATTCCAAG+AGG + tig0020424:28415-28434 Msa1381220:CDS 50.0%
! GCAACAGGCATTGTTCCTCT+TGG - tig0020424:28433-28452 None:intergenic 50.0%
AGAGGAACAATGCCTGTTGC+TGG + tig0020424:28433-28452 Msa1381220:CDS 50.0%
!! CAGTAAGGAAGAGCATCAGC+AGG - tig0020424:28460-28479 None:intergenic 50.0%
TGAGGTGGCATGTAGCAGTA+AGG - tig0020424:28475-28494 None:intergenic 50.0%
GACACCAGCAGTTCCAACTT+GGG - tig0020424:28506-28525 None:intergenic 50.0%
TGACACCAGCAGTTCCAACT+TGG - tig0020424:28507-28526 None:intergenic 50.0%
GGCTGCTGAGCATACATGTT+TGG - tig0020424:28553-28572 None:intergenic 50.0%
!! TTGTGGTGCCATGTAGGGAT+GGG - tig0020424:28575-28594 None:intergenic 50.0%
!! TTTGTGGTGCCATGTAGGGA+TGG - tig0020424:28576-28595 None:intergenic 50.0%
!! CCACATTTGTGGTGCCATGT+AGG - tig0020424:28581-28600 None:intergenic 50.0%
CCTACATGGCACCACAAATG+TGG + tig0020424:28578-28597 Msa1381220:CDS 50.0%
! TTTCCTGGCTTGCAGATTGC+AGG + tig0020424:28905-28924 Msa1381220:intron 50.0%
! TTCCTGGCTTGCAGATTGCA+GGG + tig0020424:28906-28925 Msa1381220:intron 50.0%
CAGATTGCAGGGAACTGCAA+TGG + tig0020424:28917-28936 Msa1381220:intron 50.0%
GATTGCAGGGAACTGCAATG+GGG + tig0020424:28919-28938 Msa1381220:intron 50.0%
ATTGCAGGGAACTGCAATGG+GGG + tig0020424:28920-28939 Msa1381220:three_prime_UTR 50.0%
CGATCATGACACACGGTTGG+GGG + tig0020424:27615-27634 Msa1381220:intron 55.0%
!! GTTGGGGGCACTGCAATAGA+GGG + tig0020424:27630-27649 Msa1381220:intron 55.0%
CAACCCCCATTTGCGGATTC+TGG - tig0020424:27883-27902 None:intergenic 55.0%
CTACCGACAACCCCCATTTG+CGG - tig0020424:27890-27909 None:intergenic 55.0%
CAAATGGGGGTTGTCGGTAG+CGG + tig0020424:27890-27909 Msa1381220:CDS 55.0%
AGCGGCAGTCAAATGCCGTA+CGG + tig0020424:27908-27927 Msa1381220:CDS 55.0%
GTTGCTGCTGTTGCTGCTGA+TGG - tig0020424:28066-28085 None:intergenic 55.0%
CAAGAACCACAGCCTTCCTC+TGG + tig0020424:28150-28169 Msa1381220:CDS 55.0%
TGGAGTTAACCCTGCGCTCA+TGG + tig0020424:28269-28288 Msa1381220:CDS 55.0%
AGTGTGGTTCCATGAGCGCA+GGG - tig0020424:28281-28300 None:intergenic 55.0%
CACGAGGCACAATGTCAACC+AGG - tig0020424:28378-28397 None:intergenic 55.0%
GGTTGACATTGTGCCTCGTG+AGG + tig0020424:28378-28397 Msa1381220:CDS 55.0%
ACCAGCAGTTCCAACTTGGG+AGG - tig0020424:28503-28522 None:intergenic 55.0%
!! TGTGGTGCCATGTAGGGATG+GGG - tig0020424:28574-28593 None:intergenic 55.0%
ACAAATGTGGCCACAGCCTC+CGG + tig0020424:28591-28610 Msa1381220:CDS 55.0%
TTCCCTGCAATCTGCAAGCC+AGG - tig0020424:28911-28930 None:intergenic 55.0%
!! GGTTGGGGGCACTGCAATAG+AGG + tig0020424:27629-27648 Msa1381220:intron 60.0%
GGGGCCCCAGTCATTTGGTT+TGG - tig0020424:27950-27969 None:intergenic 60.0%
AACCAAATGACTGGGGCCCC+AGG + tig0020424:27950-27969 Msa1381220:CDS 60.0%
ATCCTGGGGCCCCAGTCATT+TGG - tig0020424:27955-27974 None:intergenic 60.0%
CAGTCCACTGGTCAACCTGC+TGG + tig0020424:28001-28020 Msa1381220:CDS 60.0%
! AGCTCCAGCAGGTTGACCAG+TGG - tig0020424:28008-28027 None:intergenic 60.0%
TCAACCTGCTGGAGCTCAGC+TGG + tig0020424:28012-28031 Msa1381220:CDS 60.0%
CAACCTGCTGGAGCTCAGCT+GGG + tig0020424:28013-28032 Msa1381220:CDS 60.0%
ACCACAGCCTTCCTCTGGCA+AGG + tig0020424:28155-28174 Msa1381220:CDS 60.0%
TCCTTGCCAGAGGAAGGCTG+TGG - tig0020424:28159-28178 None:intergenic 60.0%
CAGTGTGGTTCCATGAGCGC+AGG - tig0020424:28282-28301 None:intergenic 60.0%
GCATCAGCAGGACCAGCAAC+AGG - tig0020424:28448-28467 None:intergenic 60.0%
! ACTTGGGAGGCATGCTGAGG+TGG - tig0020424:28490-28509 None:intergenic 60.0%
! CCAACTTGGGAGGCATGCTG+AGG - tig0020424:28493-28512 None:intergenic 60.0%
CCTCAGCATGCCTCCCAAGT+TGG + tig0020424:28490-28509 Msa1381220:CDS 60.0%
! GCCTCCCAAGTTGGAACTGC+TGG + tig0020424:28499-28518 Msa1381220:CDS 60.0%
TCAGCAGCCCCATCCCTACA+TGG + tig0020424:28564-28583 Msa1381220:CDS 60.0%
GGAGGCTGTGGCCACATTTG+TGG - tig0020424:28592-28611 None:intergenic 60.0%
! GGAGATGGTGGTCGCTGTTC+CGG - tig0020424:28613-28632 None:intergenic 60.0%
CAGGGAACTGCAATGGGGGT+GGG + tig0020424:28924-28943 Msa1381220:three_prime_UTR 60.0%
! GTTGGGGGCATGCAGTCCAC+TGG + tig0020424:27989-28008 Msa1381220:CDS 65.0%
TGACCCAGCTGAGCTCCAGC+AGG - tig0020424:28019-28038 None:intergenic 65.0%
! GATGGTGGTCGCTGTTCCGG+AGG - tig0020424:28610-28629 None:intergenic 65.0%
GCAGGGAACTGCAATGGGGG+TGG + tig0020424:28923-28942 Msa1381220:three_prime_UTR 65.0%
GGTCGCTGTTCCGGAGGCTG+TGG - tig0020424:28604-28623 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
tig0020424 gene 26576 29256 26576 ID=Msa1381220;Name=Msa1381220
tig0020424 mRNA 26576 29256 26576 ID=Msa1381220-mRNA-1;Parent=Msa1381220;Name=Msa1381220-mRNA-1;_AED=0.01;_eAED=0.01;_QI=93|1|1|1|0|0|4|359|260
tig0020424 exon 26576 26624 26576 ID=Msa1381220-mRNA-1:exon:7438;Parent=Msa1381220-mRNA-1
tig0020424 exon 26901 26943 26901 ID=Msa1381220-mRNA-1:exon:7439;Parent=Msa1381220-mRNA-1
tig0020424 exon 27859 28664 27859 ID=Msa1381220-mRNA-1:exon:7440;Parent=Msa1381220-mRNA-1
tig0020424 exon 28920 29256 28920 ID=Msa1381220-mRNA-1:exon:7441;Parent=Msa1381220-mRNA-1
tig0020424 five_prime_UTR 26576 26624 26576 ID=Msa1381220-mRNA-1:five_prime_utr;Parent=Msa1381220-mRNA-1
tig0020424 five_prime_UTR 26901 26943 26901 ID=Msa1381220-mRNA-1:five_prime_utr;Parent=Msa1381220-mRNA-1
tig0020424 five_prime_UTR 27859 27859 27859 ID=Msa1381220-mRNA-1:five_prime_utr;Parent=Msa1381220-mRNA-1
tig0020424 CDS 27860 28642 27860 ID=Msa1381220-mRNA-1:cds;Parent=Msa1381220-mRNA-1
tig0020424 three_prime_UTR 28643 28664 28643 ID=Msa1381220-mRNA-1:three_prime_utr;Parent=Msa1381220-mRNA-1
tig0020424 three_prime_UTR 28920 29256 28920 ID=Msa1381220-mRNA-1:three_prime_utr;Parent=Msa1381220-mRNA-1
Gene Sequence

>Msa1381220

ATGGATCATCAAGGGCACAACCAGAATCCGCAAATGGGGGTTGTCGGTAGCGGCAGTCAAATGCCGTACGGATCTAACCCATATCAGCCAAACCAAATGACTGGGGCCCCAGGATCCGTTGTTACATCAGTTGGGGGCATGCAGTCCACTGGTCAACCTGCTGGAGCTCAGCTGGGTCAGCATCAACTTGCTTACCAGCATATCCATCAGCAGCAACAGCAGCAACTTCAACAACAGCTGCAATCCTTCTGGTCAAACCAGTACCAGGAAATTGAAAAGGTTACTGATTTCAAGAACCACAGCCTTCCTCTGGCAAGGATCAAGAAGATCATGAAGGCTGATGAGGATGTTAGAATGATATCAGCTGAAGCACCTGTCATATTTGCAAGGGCCTGTGAGATGTTCATATTGGAGTTAACCCTGCGCTCATGGAACCACACTGAAGAGAACAAAAGGAGGACACTTCAGAAGAATGATATTGCTGCTGCAATCACTAGGACTGATATCTTTGATTTCCTGGTTGACATTGTGCCTCGTGAGGACCTGAAAGATGAAGTGCTGGCTTCAATTCCAAGAGGAACAATGCCTGTTGCTGGTCCTGCTGATGCTCTTCCTTACTGCTACATGCCACCTCAGCATGCCTCCCAAGTTGGAACTGCTGGTGTCATAATGGGAAAGCCTGTGATGGACCCAAACATGTATGCTCAGCAGCCCCATCCCTACATGGCACCACAAATGTGGCCACAGCCTCCGGAACAGCGACCACCATCTCCAGATCACTAG
Protein sequence

>Msa1381220

MDHQGHNQNPQMGVVGSGSQMPYGSNPYQPNQMTGAPGSVVTSVGGMQSTGQPAGAQLGQHQLAYQHIHQQQQQQLQQQLQSFWSNQYQEIEKVTDFKNHSLPLARIKKIMKADEDVRMISAEAPVIFARACEMFILELTLRSWNHTEENKRRTLQKNDIAAAITRTDIFDFLVDIVPREDLKDEVLASIPRGTMPVAGPADALPYCYMPPQHASQVGTAGVIMGKPVMDPNMYAQQPHPYMAPQMWPQPPEQRPPSPDH*