AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1131210

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1131210 MtrunA17_Chr8g0374151 77.720 193 2 1 1 152 1 193 1.05e-102 293
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1131210 AT5G66930.2 57.732 194 36 5 1 152 1 190 7.24e-66 200
Msa1131210 AT5G66930.3 71.831 142 35 4 12 152 89 226 1.88e-65 200
Msa1131210 AT5G66930.1 55.319 141 20 3 1 99 1 140 1.04e-41 137

Find 73 sgRNAs with CRISPR-Local

Find 165 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACATCAACTTGTATAATTT+TGG 0.124407 7_3:+83767511 Msa1131210:three_prime_UTR
TGATAGTAATTTAGTGTAAT+TGG 0.217983 7_3:+83765253 Msa1131210:five_prime_UTR
CGATGTGTTGTTGTCTTTAA+AGG 0.223217 7_3:+83767467 Msa1131210:three_prime_UTR
CAAACTTCACAGTTCATAAT+TGG 0.236403 7_3:-83765471 None:intergenic
CAAAGTGCCGATTACTTCTA+TGG 0.281070 7_3:-83765320 None:intergenic
AGGCATCTTGGTTCAGTAAC+AGG 0.296164 7_3:+83766926 Msa1131210:CDS
ACAAGTTAATACAAATTAAA+TGG 0.300895 7_3:-83765276 None:intergenic
CAGGATTGAACGTTTGTATT+GGG 0.340721 7_3:+83766945 Msa1131210:CDS
TTATTTGGTTTGCACTTGAA+TGG 0.357360 7_3:-83767679 None:intergenic
GGATGAAACCCTAGGTTGAT+TGG 0.361003 7_3:-83765450 None:intergenic
GAAATAGCACCCTCTAGATT+TGG 0.369910 7_3:-83767293 None:intergenic
GGATGACTGTTCTGCTTTGT+TGG 0.374728 7_3:+83767428 Msa1131210:three_prime_UTR
GATGAAACCCTAGGTTGATT+GGG 0.376517 7_3:-83765449 None:intergenic
GAGAACTGTACGAAACTATT+AGG 0.382655 7_3:+83767626 Msa1131210:three_prime_UTR
AGTTCTCCTTTATGTTTGAT+AGG 0.386226 7_3:-83767610 None:intergenic
AATTGAGCAGTTTATTTGTT+GGG 0.392401 7_3:+83766031 Msa1131210:CDS
GTGCGTGTGAAGGGTTTAGA+AGG 0.402131 7_3:-83765379 None:intergenic
CATTCTAAATTTGTTGATCC+AGG 0.409933 7_3:+83767030 Msa1131210:CDS
TTCTTTGGGAAATCAGATAT+AGG 0.412917 7_3:+83767547 Msa1131210:three_prime_UTR
TAATTACAACGGGATATGAT+TGG 0.415458 7_3:-83767363 None:intergenic
ACAGGATTGAACGTTTGTAT+TGG 0.421210 7_3:+83766944 Msa1131210:CDS
GGCAATATAAACTACAATAT+AGG 0.425910 7_3:-83767657 None:intergenic
AATGTATGAGAGAAAATTAG+AGG 0.433111 7_3:+83767772 Msa1131210:three_prime_UTR
CCCTCTAGATTTGGTATTGG+CGG 0.433639 7_3:-83767284 None:intergenic
TAAACATACCTAGGACAGTT+TGG 0.434428 7_3:+83767808 Msa1131210:three_prime_UTR
TATTTGGTTTGCACTTGAAT+GGG 0.453048 7_3:-83767678 None:intergenic
ATTCATGACCAAACTGTCCT+AGG 0.460668 7_3:-83767816 None:intergenic
CTAGGTATTGTAATTTCATA+CGG 0.474349 7_3:-83767320 None:intergenic
GCACCCTCTAGATTTGGTAT+TGG 0.474637 7_3:-83767287 None:intergenic
AAATTGAGCAGTTTATTTGT+TGG 0.479900 7_3:+83766030 Msa1131210:CDS
ATGAGATGCATAATTACAAC+GGG 0.480053 7_3:-83767373 None:intergenic
GAAATCAGATATAGGATTCA+AGG 0.482038 7_3:+83767555 Msa1131210:three_prime_UTR
ATTGCAACCATAGAAGTAAT+CGG 0.484851 7_3:+83765313 Msa1131210:five_prime_UTR
TAGAATGCGATTTCAACTGC+TGG 0.486426 7_3:-83766996 None:intergenic
GATTTGCCTATCAAACATAA+AGG 0.487879 7_3:+83767604 Msa1131210:three_prime_UTR
AACGTTTGTATTGGGAGCAA+TGG 0.491465 7_3:+83766953 Msa1131210:CDS
AAATTATACAAGTTGATGTA+TGG 0.495983 7_3:-83767508 None:intergenic
TATATCTGATTTCCCAAAGA+AGG 0.496986 7_3:-83767545 None:intergenic
AATTACAACGGGATATGATT+GGG 0.497946 7_3:-83767362 None:intergenic
TAAATTCGTCAACGAGAAGA+AGG 0.500734 7_3:+83767253 Msa1131210:CDS
GATATGCTTATCTTTCTATG+AGG 0.522607 7_3:+83766891 Msa1131210:intron
GGTGCAATGTGGAGAGGTGG+AGG 0.545332 7_3:+83765986 Msa1131210:intron
AGGAATAGCGTGCGTGTGAA+GGG 0.547622 7_3:-83765388 None:intergenic
GAGGAATAGCGTGCGTGTGA+AGG 0.552519 7_3:-83765389 None:intergenic
TGAGCAGTTTATTTGTTGGG+TGG 0.552749 7_3:+83766034 Msa1131210:CDS
ATGAAACCCTAGGTTGATTG+GGG 0.564707 7_3:-83765448 None:intergenic
TCATAATTGGATGAAACCCT+AGG 0.567267 7_3:-83765458 None:intergenic
CGTGTGGATCGGAGAAGAAG+AGG 0.567756 7_3:-83765408 None:intergenic
CTTATTGAGTAAACATACCT+AGG 0.570266 7_3:+83767799 Msa1131210:three_prime_UTR
ACTTCTATGGTTGCAATCAG+AGG 0.571707 7_3:-83765307 None:intergenic
AGTTATATACGAGTTCTTTG+AGG 0.572020 7_3:-83765497 None:intergenic
CTCATGAGTAGATGGAACAT+TGG 0.573528 7_3:+83767166 Msa1131210:intron
ATGAGTAGATGGAACATTGG+AGG 0.574356 7_3:+83767169 Msa1131210:intron
TGGATATGAGAATGCTAAGG+TGG 0.575908 7_3:+83767711 Msa1131210:three_prime_UTR
TATATACGAGTTCTTTGAGG+TGG 0.577021 7_3:-83765494 None:intergenic
GGAGGAGTGATCGTGTGGAT+CGG 0.578711 7_3:-83765419 None:intergenic
GAAGAGGAGGAGTGATCGTG+TGG 0.585535 7_3:-83765424 None:intergenic
AGAATGCGATTTCAACTGCT+GGG 0.602143 7_3:-83766995 None:intergenic
TTTGTTGTGTAGGTGCAATG+TGG 0.609270 7_3:+83765975 Msa1131210:intron
TCTAAATTTGTTGATCCAGG+AGG 0.611659 7_3:+83767033 Msa1131210:CDS
CGTATGAAATTACAATACCT+AGG 0.614228 7_3:+83767321 Msa1131210:CDS
TGTGTAGGTGCAATGTGGAG+AGG 0.615453 7_3:+83765980 Msa1131210:intron
GTAGGTGCAATGTGGAGAGG+TGG 0.624583 7_3:+83765983 Msa1131210:intron
TCCGCCAATACCAAATCTAG+AGG 0.635248 7_3:+83767283 Msa1131210:CDS
TAGTGGATATGAGAATGCTA+AGG 0.642903 7_3:+83767708 Msa1131210:three_prime_UTR
TATGAGATGCATAATTACAA+CGG 0.645493 7_3:-83767374 None:intergenic
ACTTGAAGTAGCACTTCGTG+AGG 0.649855 7_3:+83767214 Msa1131210:CDS
CCGCCAATACCAAATCTAGA+GGG 0.660550 7_3:+83767284 Msa1131210:CDS
AGAGTATACAGATCTTACCT+AGG 0.671659 7_3:-83767338 None:intergenic
GTGGATGAACAAATTGCTTG+TGG 0.682888 7_3:+83767577 Msa1131210:three_prime_UTR
GAAACCCTAGGTTGATTGGG+GGG 0.698746 7_3:-83765446 None:intergenic
ATCAGATATAGGATTCAAGG+TGG 0.706606 7_3:+83767558 Msa1131210:three_prime_UTR
TGAAACCCTAGGTTGATTGG+GGG 0.712352 7_3:-83765447 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAGACAAAATAAATAAATAA+TGG - chr7_3:83766210-83766229 None:intergenic 10.0%
!! AATTTAAGTATAAAATCATA+CGG + chr7_3:83766336-83766355 Msa1131210:intron 10.0%
!! ACAAGTTAATACAAATTAAA+TGG - chr7_3:83765279-83765298 None:intergenic 15.0%
!! AAATTGAAAAATGATTAAGA+AGG - chr7_3:83765571-83765590 None:intergenic 15.0%
!!! ATATTTTGATATCTTTGATT+CGG + chr7_3:83767407-83767426 Msa1131210:three_prime_UTR 15.0%
!! TGATAGTAATTTAGTGTAAT+TGG + chr7_3:83765253-83765272 Msa1131210:five_prime_UTR 20.0%
!!! TAATCATTTTTCAATTTTGC+AGG + chr7_3:83765574-83765593 Msa1131210:intron 20.0%
!! TTGAAATAAAAAACGTACAT+AGG - chr7_3:83765717-83765736 None:intergenic 20.0%
!!! TTTGTCTAAATACATCATTT+TGG + chr7_3:83766222-83766241 Msa1131210:intron 20.0%
!!! CATTTTTTATATCTTTGAGT+CGG + chr7_3:83766249-83766268 Msa1131210:intron 20.0%
!! AATGTTGATGTAAAATAAAC+AGG + chr7_3:83766799-83766818 Msa1131210:intron 20.0%
!! TGTAAAATAAACAGGAATAT+AGG + chr7_3:83766807-83766826 Msa1131210:intron 20.0%
!! GTAAAATAAACAGGAATATA+GGG + chr7_3:83766808-83766827 Msa1131210:intron 20.0%
!!! TTGTAATCAAGTTTTCTTAT+TGG + chr7_3:83766848-83766867 Msa1131210:intron 20.0%
!!! TGTAATCAAGTTTTCTTATT+GGG + chr7_3:83766849-83766868 Msa1131210:intron 20.0%
!!! AAATTATACAAGTTGATGTA+TGG - chr7_3:83767511-83767530 None:intergenic 20.0%
!!! TACATCAACTTGTATAATTT+TGG + chr7_3:83767511-83767530 Msa1131210:three_prime_UTR 20.0%
!!! TCCAAGAAAATATTAGTTTT+TGG + chr7_3:83767745-83767764 Msa1131210:three_prime_UTR 20.0%
!!! TCCAAAAACTAATATTTTCT+TGG - chr7_3:83767749-83767768 None:intergenic 20.0%
! TTGAATGTAATCACATTTGT+CGG + chr7_3:83765844-83765863 Msa1131210:intron 25.0%
!!! CATTTCAATTTCCAGTTTTT+TGG + chr7_3:83765935-83765954 Msa1131210:intron 25.0%
! AAATTGAGCAGTTTATTTGT+TGG + chr7_3:83766030-83766049 Msa1131210:CDS 25.0%
! AATTGAGCAGTTTATTTGTT+GGG + chr7_3:83766031-83766050 Msa1131210:CDS 25.0%
! TGTATGATCTTCTTTAAACA+TGG - chr7_3:83766391-83766410 None:intergenic 25.0%
!! GATTTGCTTATCAAACATAA+AGG + chr7_3:83766449-83766468 Msa1131210:intron 25.0%
!!! TTTCAGGAAAGATTAGTTTT+TGG + chr7_3:83766582-83766601 Msa1131210:intron 25.0%
!! AGAATTTAGTTTATCGAACA+AGG - chr7_3:83767071-83767090 None:intergenic 25.0%
!! CTAGGTATTGTAATTTCATA+CGG - chr7_3:83767323-83767342 None:intergenic 25.0%
! TATGAGATGCATAATTACAA+CGG - chr7_3:83767377-83767396 None:intergenic 25.0%
! GGCAATATAAACTACAATAT+AGG - chr7_3:83767660-83767679 None:intergenic 25.0%
!! AAACCAAATAAAAAGTGTAG+TGG + chr7_3:83767691-83767710 Msa1131210:three_prime_UTR 25.0%
!!! TATCCACTACACTTTTTATT+TGG - chr7_3:83767697-83767716 None:intergenic 25.0%
! AATGTATGAGAGAAAATTAG+AGG + chr7_3:83767772-83767791 Msa1131210:three_prime_UTR 25.0%
ATTGCAACCATAGAAGTAAT+CGG + chr7_3:83765313-83765332 Msa1131210:five_prime_UTR 30.0%
CAAACTTCACAGTTCATAAT+TGG - chr7_3:83765474-83765493 None:intergenic 30.0%
AGTTATATACGAGTTCTTTG+AGG - chr7_3:83765500-83765519 None:intergenic 30.0%
GAAAAATGATTAAGAAGGAG+AGG - chr7_3:83765566-83765585 None:intergenic 30.0%
TAATGGTGTGCAATATACAT+CGG - chr7_3:83765631-83765650 None:intergenic 30.0%
AGCACAATTTACCAAAAAAC+TGG - chr7_3:83765949-83765968 None:intergenic 30.0%
!!! TGCTAAGTTTTTTGTTGTGT+AGG + chr7_3:83765965-83765984 Msa1131210:intron 30.0%
!! ATTTAGTGTCAGTTCTAGAT+TGG + chr7_3:83766146-83766165 Msa1131210:intron 30.0%
TACGGCAAGTTGTATAATTA+TGG + chr7_3:83766354-83766373 Msa1131210:intron 30.0%
ATCATACATGTGATTCAAGA+TGG + chr7_3:83766403-83766422 Msa1131210:intron 30.0%
GATTTGCCTATCAAACATAA+AGG + chr7_3:83766484-83766503 Msa1131210:intron 30.0%
ACTTCTCCTTTATGTTTGAT+AGG - chr7_3:83766458-83766477 None:intergenic 30.0%
AAAGATGTGAAAAATCACCT+AGG + chr7_3:83766647-83766666 Msa1131210:intron 30.0%
TAACATGACTAAATCATCCT+AGG - chr7_3:83766667-83766686 None:intergenic 30.0%
GATATGCTTATCTTTCTATG+AGG + chr7_3:83766891-83766910 Msa1131210:intron 30.0%
CTATGAGGTTAAAAACAAAC+AGG + chr7_3:83766906-83766925 Msa1131210:CDS 30.0%
TTAAAAACAAACAGGCATCT+TGG + chr7_3:83766914-83766933 Msa1131210:CDS 30.0%
!! CATTCTAAATTTGTTGATCC+AGG + chr7_3:83767030-83767049 Msa1131210:CDS 30.0%
AAGTGACAAATAAAGAGTGA+GGG - chr7_3:83767135-83767154 None:intergenic 30.0%
AAAGTGACAAATAAAGAGTG+AGG - chr7_3:83767136-83767155 None:intergenic 30.0%
CGTATGAAATTACAATACCT+AGG + chr7_3:83767321-83767340 Msa1131210:CDS 30.0%
AATTACAACGGGATATGATT+GGG - chr7_3:83767365-83767384 None:intergenic 30.0%
TAATTACAACGGGATATGAT+TGG - chr7_3:83767366-83767385 None:intergenic 30.0%
ATGAGATGCATAATTACAAC+GGG - chr7_3:83767376-83767395 None:intergenic 30.0%
! GTATCTTTTGATCCTTCTTT+GGG + chr7_3:83767533-83767552 Msa1131210:three_prime_UTR 30.0%
TATATCTGATTTCCCAAAGA+AGG - chr7_3:83767548-83767567 None:intergenic 30.0%
! TTCTTTGGGAAATCAGATAT+AGG + chr7_3:83767547-83767566 Msa1131210:three_prime_UTR 30.0%
GAAATCAGATATAGGATTCA+AGG + chr7_3:83767555-83767574 Msa1131210:three_prime_UTR 30.0%
GATTTGCCTATCAAACATAA+AGG + chr7_3:83766484-83766503 Msa1131210:intron 30.0%
AGTTCTCCTTTATGTTTGAT+AGG - chr7_3:83767613-83767632 None:intergenic 30.0%
!! TATTTGGTTTGCACTTGAAT+GGG - chr7_3:83767681-83767700 None:intergenic 30.0%
!! TTATTTGGTTTGCACTTGAA+TGG - chr7_3:83767682-83767701 None:intergenic 30.0%
CTTATTGAGTAAACATACCT+AGG + chr7_3:83767799-83767818 Msa1131210:three_prime_UTR 30.0%
TCATAATTGGATGAAACCCT+AGG - chr7_3:83765461-83765480 None:intergenic 35.0%
! TATATACGAGTTCTTTGAGG+TGG - chr7_3:83765497-83765516 None:intergenic 35.0%
TGCGAATAGTAGAAAAAAGG+GGG - chr7_3:83765543-83765562 None:intergenic 35.0%
GTGCGAATAGTAGAAAAAAG+GGG - chr7_3:83765544-83765563 None:intergenic 35.0%
GGTGCGAATAGTAGAAAAAA+GGG - chr7_3:83765545-83765564 None:intergenic 35.0%
AGGTGCGAATAGTAGAAAAA+AGG - chr7_3:83765546-83765565 None:intergenic 35.0%
TAACGCACGATGGAAAATAA+TGG - chr7_3:83765648-83765667 None:intergenic 35.0%
! ATTATTTTCCATCGTGCGTT+AGG + chr7_3:83765647-83765666 Msa1131210:intron 35.0%
AGTTCCAAGTCTACATCTTT+GGG - chr7_3:83765683-83765702 None:intergenic 35.0%
ACATCCAAACAAAAAGAGTC+AGG + chr7_3:83766061-83766080 Msa1131210:CDS 35.0%
! CTAACCTGACTCTTTTTGTT+TGG - chr7_3:83766068-83766087 None:intergenic 35.0%
TCAGGTTAGATATTTGCTTG+TGG + chr7_3:83766079-83766098 Msa1131210:intron 35.0%
! TGCTTGTGGATTTTTACTCT+TGG + chr7_3:83766093-83766112 Msa1131210:intron 35.0%
! GCTTGTGGATTTTTACTCTT+GGG + chr7_3:83766094-83766113 Msa1131210:intron 35.0%
CGATGTGATGTTGTCTATAA+AGG + chr7_3:83766310-83766329 Msa1131210:intron 35.0%
!! GCAAATCAAATAAAGTGCTG+TGG + chr7_3:83766529-83766548 Msa1131210:intron 35.0%
!! AGTCCAATGGCTTTAATGAA+AGG + chr7_3:83766689-83766708 Msa1131210:intron 35.0%
ACTCCTTTCATTAAAGCCAT+TGG - chr7_3:83766695-83766714 None:intergenic 35.0%
!! AATGGCTTTAATGAAAGGAG+TGG + chr7_3:83766694-83766713 Msa1131210:intron 35.0%
AATAAACAGGAATATAGGGC+AGG + chr7_3:83766812-83766831 Msa1131210:intron 35.0%
ATAAACAGGAATATAGGGCA+GGG + chr7_3:83766813-83766832 Msa1131210:intron 35.0%
ACAGGATTGAACGTTTGTAT+TGG + chr7_3:83766944-83766963 Msa1131210:CDS 35.0%
CAGGATTGAACGTTTGTATT+GGG + chr7_3:83766945-83766964 Msa1131210:CDS 35.0%
!! TCTAAATTTGTTGATCCAGG+AGG + chr7_3:83767033-83767052 Msa1131210:CDS 35.0%
TCGTATTTCTCATGAGTAGA+TGG + chr7_3:83767158-83767177 Msa1131210:intron 35.0%
TAAATTCGTCAACGAGAAGA+AGG + chr7_3:83767253-83767272 Msa1131210:CDS 35.0%
AGAGTATACAGATCTTACCT+AGG - chr7_3:83767341-83767360 None:intergenic 35.0%
! CGATGTGTTGTTGTCTTTAA+AGG + chr7_3:83767467-83767486 Msa1131210:three_prime_UTR 35.0%
! GGTATCTTTTGATCCTTCTT+TGG + chr7_3:83767532-83767551 Msa1131210:three_prime_UTR 35.0%
ATCAGATATAGGATTCAAGG+TGG + chr7_3:83767558-83767577 Msa1131210:three_prime_UTR 35.0%
GAGAACTGTACGAAACTATT+AGG + chr7_3:83767626-83767645 Msa1131210:three_prime_UTR 35.0%
! TAGTGGATATGAGAATGCTA+AGG + chr7_3:83767708-83767727 Msa1131210:three_prime_UTR 35.0%
TAAACATACCTAGGACAGTT+TGG + chr7_3:83767808-83767827 Msa1131210:three_prime_UTR 35.0%
ACTTCTATGGTTGCAATCAG+AGG - chr7_3:83765310-83765329 None:intergenic 40.0%
!! CAAAGTGCCGATTACTTCTA+TGG - chr7_3:83765323-83765342 None:intergenic 40.0%
!! ATGAAACCCTAGGTTGATTG+GGG - chr7_3:83765451-83765470 None:intergenic 40.0%
!! GATGAAACCCTAGGTTGATT+GGG - chr7_3:83765452-83765471 None:intergenic 40.0%
GCGAATAGTAGAAAAAAGGG+GGG - chr7_3:83765542-83765561 None:intergenic 40.0%
GAGTTCCAAGTCTACATCTT+TGG - chr7_3:83765684-83765703 None:intergenic 40.0%
! GGTGCTACAAAGATTGTGAT+AGG - chr7_3:83765748-83765767 None:intergenic 40.0%
! ACACTTTCGATCAGAAGTGT+CGG - chr7_3:83765769-83765788 None:intergenic 40.0%
! ACACTTCTGATCGAAAGTGT+CGG + chr7_3:83765769-83765788 Msa1131210:intron 40.0%
TTGTACCACCTTATGATCGA+AGG - chr7_3:83765894-83765913 None:intergenic 40.0%
! TTTGTTGTGTAGGTGCAATG+TGG + chr7_3:83765975-83765994 Msa1131210:intron 40.0%
! TGAGCAGTTTATTTGTTGGG+TGG + chr7_3:83766034-83766053 Msa1131210:CDS 40.0%
CTGTGGATATGAGAATGCTA+AGG + chr7_3:83766546-83766565 Msa1131210:intron 40.0%
TGGATATGAGAATGCTAAGG+TGG + chr7_3:83766549-83766568 Msa1131210:intron 40.0%
TAGTCATGTTAGCAGTCCAA+TGG + chr7_3:83766676-83766695 Msa1131210:intron 40.0%
!! GGAGTGGAGTACTCTTTTAT+TGG + chr7_3:83766710-83766729 Msa1131210:intron 40.0%
AACGTTTGTATTGGGAGCAA+TGG + chr7_3:83766953-83766972 Msa1131210:CDS 40.0%
AGAATGCGATTTCAACTGCT+GGG - chr7_3:83766998-83767017 None:intergenic 40.0%
TAGAATGCGATTTCAACTGC+TGG - chr7_3:83766999-83767018 None:intergenic 40.0%
CTCATGAGTAGATGGAACAT+TGG + chr7_3:83767166-83767185 Msa1131210:intron 40.0%
ATGAGTAGATGGAACATTGG+AGG + chr7_3:83767169-83767188 Msa1131210:intron 40.0%
GAAATAGCACCCTCTAGATT+TGG - chr7_3:83767296-83767315 None:intergenic 40.0%
GTGGATGAACAAATTGCTTG+TGG + chr7_3:83767577-83767596 Msa1131210:three_prime_UTR 40.0%
TGGATATGAGAATGCTAAGG+TGG + chr7_3:83766549-83766568 Msa1131210:intron 40.0%
ATTCATGACCAAACTGTCCT+AGG - chr7_3:83767819-83767838 None:intergenic 40.0%
!! TGAAACCCTAGGTTGATTGG+GGG - chr7_3:83765450-83765469 None:intergenic 45.0%
!! GGATGAAACCCTAGGTTGAT+TGG - chr7_3:83765453-83765472 None:intergenic 45.0%
ACATTTGTCGGTCTCCAACA+CGG + chr7_3:83765856-83765875 Msa1131210:intron 45.0%
CCCTAACTATGTGAACACAG+CGG - chr7_3:83766122-83766141 None:intergenic 45.0%
CCGCTGTGTTCACATAGTTA+GGG + chr7_3:83766119-83766138 Msa1131210:intron 45.0%
GAATGATCGTTCTGCTTGGT+TGG + chr7_3:83766271-83766290 Msa1131210:intron 45.0%
AGGTGGCTTAGTGTACTTTC+AGG + chr7_3:83766566-83766585 Msa1131210:intron 45.0%
AGGCATCTTGGTTCAGTAAC+AGG + chr7_3:83766926-83766945 Msa1131210:CDS 45.0%
AGGAAAGAAACTTCACCTCC+TGG - chr7_3:83767051-83767070 None:intergenic 45.0%
ACTGTGCAAGTAGTTCCTTC+TGG + chr7_3:83767107-83767126 Msa1131210:intron 45.0%
ACTTGAAGTAGCACTTCGTG+AGG + chr7_3:83767214-83767233 Msa1131210:CDS 45.0%
TCCGCCAATACCAAATCTAG+AGG + chr7_3:83767283-83767302 Msa1131210:CDS 45.0%
! CCCTCTAGATTTGGTATTGG+CGG - chr7_3:83767287-83767306 None:intergenic 45.0%
CCGCCAATACCAAATCTAGA+GGG + chr7_3:83767284-83767303 Msa1131210:CDS 45.0%
! GCACCCTCTAGATTTGGTAT+TGG - chr7_3:83767290-83767309 None:intergenic 45.0%
GGATGACTGTTCTGCTTTGT+TGG + chr7_3:83767428-83767447 Msa1131210:three_prime_UTR 45.0%
GTGCGTGTGAAGGGTTTAGA+AGG - chr7_3:83765382-83765401 None:intergenic 50.0%
AGGAATAGCGTGCGTGTGAA+GGG - chr7_3:83765391-83765410 None:intergenic 50.0%
TCTTCCCCCCAATCAACCTA+GGG + chr7_3:83765442-83765461 Msa1131210:five_prime_UTR 50.0%
!! GAAACCCTAGGTTGATTGGG+GGG - chr7_3:83765449-83765468 None:intergenic 50.0%
GTAGAAAAAAGGGGGGTGAG+AGG - chr7_3:83765535-83765554 None:intergenic 50.0%
GCGTCCCAAAGATGTAGACT+TGG + chr7_3:83765676-83765695 Msa1131210:intron 50.0%
!! CTGATCGAAAGTGTCGGTGT+TGG + chr7_3:83765775-83765794 Msa1131210:intron 50.0%
TGGACACGCCTTCGATCATA+AGG + chr7_3:83765883-83765902 Msa1131210:intron 50.0%
ACACGCCTTCGATCATAAGG+TGG + chr7_3:83765886-83765905 Msa1131210:intron 50.0%
TGTGTAGGTGCAATGTGGAG+AGG + chr7_3:83765980-83765999 Msa1131210:intron 50.0%
GCCGCTGTGTTCACATAGTT+AGG + chr7_3:83766118-83766137 Msa1131210:intron 50.0%
GTCGGAATGATCGTTCTGCT+TGG + chr7_3:83766267-83766286 Msa1131210:intron 50.0%
!! TAAAGAGTGAGGGTGCCAGA+AGG - chr7_3:83767125-83767144 None:intergenic 50.0%
GAGGAATAGCGTGCGTGTGA+AGG - chr7_3:83765392-83765411 None:intergenic 55.0%
CGTGTGGATCGGAGAAGAAG+AGG - chr7_3:83765411-83765430 None:intergenic 55.0%
GGAGGAGTGATCGTGTGGAT+CGG - chr7_3:83765422-83765441 None:intergenic 55.0%
GAAGAGGAGGAGTGATCGTG+TGG - chr7_3:83765427-83765446 None:intergenic 55.0%
!! CTAGGTTGATTGGGGGGAAG+AGG - chr7_3:83765443-83765462 None:intergenic 55.0%
CTCTTCCCCCCAATCAACCT+AGG + chr7_3:83765441-83765460 Msa1131210:five_prime_UTR 55.0%
GCACAAGACCTAACGCACGA+TGG - chr7_3:83765658-83765677 None:intergenic 55.0%
TCGGTCTCCAACACGGATAC+TGG + chr7_3:83765863-83765882 Msa1131210:intron 55.0%
GTAGGTGCAATGTGGAGAGG+TGG + chr7_3:83765983-83766002 Msa1131210:intron 55.0%
GGTTGATTGGGGGGAAGAGG+AGG - chr7_3:83765440-83765459 None:intergenic 60.0%
GGCGTGTCCAGTATCCGTGT+TGG - chr7_3:83765873-83765892 None:intergenic 60.0%
GGTGCAATGTGGAGAGGTGG+AGG + chr7_3:83765986-83766005 Msa1131210:intron 60.0%
Chromosome Type Strat End Strand Name
chr7_3 gene 83765219 83767852 83765219 ID=Msa1131210;Name=Msa1131210
chr7_3 mRNA 83765219 83767852 83765219 ID=Msa1131210-mRNA-1;Parent=Msa1131210;Name=Msa1131210-mRNA-1;_AED=0.19;_eAED=0.19;_QI=258|0.66|1|1|0.66|0.75|4|506|153
chr7_3 exon 83765219 83765509 83765219 ID=Msa1131210-mRNA-1:exon:24847;Parent=Msa1131210-mRNA-1
chr7_3 exon 83765987 83766082 83765987 ID=Msa1131210-mRNA-1:exon:24848;Parent=Msa1131210-mRNA-1
chr7_3 exon 83766892 83767054 83766892 ID=Msa1131210-mRNA-1:exon:24849;Parent=Msa1131210-mRNA-1
chr7_3 exon 83767177 83767852 83767177 ID=Msa1131210-mRNA-1:exon:24850;Parent=Msa1131210-mRNA-1
chr7_3 five_prime_UTR 83765219 83765476 83765219 ID=Msa1131210-mRNA-1:five_prime_utr;Parent=Msa1131210-mRNA-1
chr7_3 CDS 83765477 83765509 83765477 ID=Msa1131210-mRNA-1:cds;Parent=Msa1131210-mRNA-1
chr7_3 CDS 83765987 83766082 83765987 ID=Msa1131210-mRNA-1:cds;Parent=Msa1131210-mRNA-1
chr7_3 CDS 83766892 83767054 83766892 ID=Msa1131210-mRNA-1:cds;Parent=Msa1131210-mRNA-1
chr7_3 CDS 83767177 83767346 83767177 ID=Msa1131210-mRNA-1:cds;Parent=Msa1131210-mRNA-1
chr7_3 three_prime_UTR 83767347 83767852 83767347 ID=Msa1131210-mRNA-1:three_prime_utr;Parent=Msa1131210-mRNA-1
Gene Sequence

>Msa1131210

ATGAACTGTGAAGTTTGCCACCTCAAAGAACTCGTGCAATGTGGAGAGGTGGAGGTTGAAAACAAAATAGAAGAGAAAATTGAGCAGTTTATTTGTTGGGTGGAGAAACATCCAAACAAAAAGAGTCAGATATGCTTATCTTTCTATGAGGTTAAAAACAAACAGGCATCTTGGTTCAGTAACAGGATTGAACGTTTGTATTGGGAGCAATGGTATATTAATCTGAATGTAGCCCAGCAGTTGAAATCGCATTCTAGCAAATCTCATCATTCTAAATTTGTTGATCCAGGAGATGGAACATTGGAGGATAGAAATGCTCGAAGTGCAGCACTTGAAGTAGCACTTCGTGAGGTTTTATTTCAAATAATTAAATTCGTCAACGAGAAGAAGGATCATGTTCCGCCAATACCAAATCTAGAGGGTGCTATTTCTTTTCCGTATGAAATTACAATACCTAGGTAA
Protein sequence

>Msa1131210

MNCEVCHLKELVQCGEVEVENKIEEKIEQFICWVEKHPNKKSQICLSFYEVKNKQASWFSNRIERLYWEQWYINLNVAQQLKSHSSKSHHSKFVDPGDGTLEDRNARSAALEVALREVLFQIIKFVNEKKDHVPPIPNLEGAISFPYEITIPR*