AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1132280

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1132280 MtrunA17_Chr5g0394391 97.902 572 12 0 1 572 1 572 0.0 1152
Msa1132280 MtrunA17_Chr4g0064621 61.345 595 201 8 1 571 101 690 0.0 664
Msa1132280 MtrunA17_Chr4g0060241 56.863 561 207 13 19 569 10 545 0.0 572
Msa1132280 MtrunA17_Chr5g0442171 85.152 330 41 2 190 518 1 323 0.0 566
Msa1132280 MtrunA17_Chr5g0442161 83.721 172 27 1 8 178 7 178 4.62e-99 298
Msa1132280 MtrunA17_Chr7g0223181 37.228 505 290 10 29 523 9 496 1.32e-98 313
Msa1132280 MtrunA17_Chr4g0073741 36.853 502 296 10 29 523 8 495 2.74e-98 312
Msa1132280 MtrunA17_Chr7g0223291 37.426 505 289 10 29 523 9 496 3.06e-98 312
Msa1132280 MtrunA17_Chr4g0031321 36.634 505 293 10 29 523 9 496 1.19e-96 308
Msa1132280 MtrunA17_Chr7g0262821 36.170 517 279 11 29 523 9 496 2.34e-96 307
Msa1132280 MtrunA17_Chr4g0031301 36.832 505 292 10 29 523 9 496 2.69e-96 307
Msa1132280 MtrunA17_Chr2g0298851 35.317 521 293 14 29 530 9 504 4.88e-87 282
Msa1132280 MtrunA17_Chr2g0296391 35.798 514 300 12 29 530 9 504 5.32e-87 279
Msa1132280 MtrunA17_Chr7g0223721 34.308 513 308 12 29 530 9 503 1.42e-86 280
Msa1132280 MtrunA17_Chr8g0350731 34.142 536 321 14 8 531 16 531 5.46e-86 280
Msa1132280 MtrunA17_Chr2g0293581 35.897 507 295 13 29 523 10 498 2.18e-85 278
Msa1132280 MtrunA17_Chr7g0233311 35.882 510 307 10 29 531 9 505 3.72e-85 277
Msa1132280 MtrunA17_Chr7g0223851 35.882 510 304 13 29 530 18 512 1.24e-84 276
Msa1132280 MtrunA17_Chr7g0223621 33.704 540 328 13 29 557 9 529 1.62e-84 275
Msa1132280 MtrunA17_Chr7g0223781 33.456 544 324 14 29 554 9 532 1.42e-83 273
Msa1132280 MtrunA17_Chr8g0388761 33.779 524 298 12 19 523 29 522 1.82e-83 274
Msa1132280 MtrunA17_Chr7g0223801 34.291 522 316 13 28 539 8 512 2.76e-83 272
Msa1132280 MtrunA17_Chr8g0388671 33.015 524 308 10 16 523 26 522 6.81e-83 272
Msa1132280 MtrunA17_Chr2g0284681 33.138 513 306 11 29 523 8 501 7.19e-83 270
Msa1132280 MtrunA17_Chr7g0223711 34.653 505 305 11 28 523 8 496 2.14e-82 270
Msa1132280 MtrunA17_Chr7g0221661 35.714 504 299 11 29 523 9 496 6.42e-82 268
Msa1132280 MtrunA17_Chr7g0225671 35.340 515 299 17 29 530 16 509 2.94e-81 267
Msa1132280 MtrunA17_Chr5g0427901 35.135 518 294 15 29 523 9 507 3.41e-80 264
Msa1132280 MtrunA17_Chr7g0223741 33.789 512 314 11 29 531 9 504 8.24e-80 262
Msa1132280 MtrunA17_Chr2g0287631 36.893 515 295 13 28 530 8 504 2.15e-79 261
Msa1132280 MtrunA17_Chr7g0223751 33.270 523 317 14 29 539 9 511 6.80e-79 260
Msa1132280 MtrunA17_Chr2g0298861 33.141 519 318 14 30 531 10 516 9.64e-79 260
Msa1132280 MtrunA17_Chr7g0223821 35.271 516 298 17 29 529 9 503 1.36e-78 262
Msa1132280 MtrunA17_Chr7g0223731 33.268 514 313 15 29 531 9 503 1.93e-78 259
Msa1132280 MtrunA17_Chr4g0040931 33.399 506 308 13 29 523 9 496 9.32e-76 252
Msa1132280 MtrunA17_Chr6g0462681 31.898 511 308 11 27 521 7 493 1.64e-75 253
Msa1132280 MtrunA17_Chr7g0233301 34.442 511 282 13 29 531 9 474 3.28e-75 250
Msa1132280 MtrunA17_Chr7g0223631 34.165 521 303 14 28 539 8 497 9.32e-75 249
Msa1132280 MtrunA17_Chr6g0462601 32.495 517 301 14 27 523 7 495 1.44e-74 249
Msa1132280 MtrunA17_Chr7g0223831 34.698 513 278 16 29 530 9 475 4.10e-74 247
Msa1132280 MtrunA17_Chr7g0248151 34.884 516 303 17 29 531 9 504 3.40e-73 245
Msa1132280 MtrunA17_Chr7g0223031 32.879 514 315 14 29 531 9 503 5.20e-73 244
Msa1132280 MtrunA17_Chr7g0223541 36.986 438 249 11 96 523 33 453 3.64e-72 241
Msa1132280 MtrunA17_Chr6g0462771 32.277 505 319 12 27 521 7 498 1.53e-71 241
Msa1132280 MtrunA17_Chr6g0462641 33.268 508 310 14 27 521 7 498 1.07e-70 240
Msa1132280 MtrunA17_Chr4g0012541 32.422 512 310 13 29 530 9 494 1.15e-70 238
Msa1132280 MtrunA17_Chr7g0248131 33.784 518 308 17 29 531 9 506 4.80e-70 237
Msa1132280 MtrunA17_Chr2g0279901 31.335 517 292 11 30 523 57 533 7.63e-70 238
Msa1132280 MtrunA17_Chr4g0049501 29.433 547 314 12 5 523 29 531 5.75e-69 235
Msa1132280 MtrunA17_Chr6g0462711 32.759 522 295 16 27 521 8 500 8.64e-69 235
Msa1132280 MtrunA17_Chr7g0217971 32.745 510 295 13 29 530 9 478 1.04e-68 233
Msa1132280 MtrunA17_Chr7g0223701 32.039 515 313 12 28 523 8 504 1.31e-68 234
Msa1132280 MtrunA17_Chr7g0215261 33.592 515 275 18 29 530 9 469 1.84e-68 232
Msa1132280 MtrunA17_Chr7g0223681 34.987 383 237 6 143 523 34 406 3.13e-66 224
Msa1132280 MtrunA17_Chr6g0462611 32.401 429 276 8 96 521 24 441 9.34e-64 220
Msa1132280 MtrunA17_Chr2g0293601 31.683 505 283 14 28 521 8 461 5.23e-63 214
Msa1132280 MtrunA17_Chr2g0277021 31.827 509 308 15 27 523 69 550 1.64e-61 216
Msa1132280 MtrunA17_Chr8g0390821 30.104 578 285 19 27 530 9 541 2.91e-60 211
Msa1132280 MtrunA17_Chr7g0228281 31.128 514 278 16 29 530 70 519 3.52e-57 202
Msa1132280 MtrunA17_Chr2g0293561 33.687 377 232 9 28 397 8 373 1.35e-54 189
Msa1132280 MtrunA17_Chr2g0299821 30.063 479 290 15 30 486 10 465 1.39e-54 192
Msa1132280 MtrunA17_Chr2g0299791 33.753 397 233 12 29 414 1042 1419 1.97e-52 193
Msa1132280 MtrunA17_Chr2g0331381 33.107 441 237 16 30 457 7 402 2.50e-52 186
Msa1132280 MtrunA17_Chr6g0462861 29.600 500 310 14 29 521 9 473 2.67e-52 189
Msa1132280 MtrunA17_Chr2g0299921 32.836 402 224 10 29 411 1029 1403 2.46e-51 190
Msa1132280 MtrunA17_Chr2g0299921 33.248 391 214 11 29 399 1413 1776 2.93e-50 187
Msa1132280 MtrunA17_Chr2g0299851 31.888 392 245 10 29 413 1038 1414 3.26e-47 178
Msa1132280 MtrunA17_Chr2g0299881 32.242 397 241 11 30 414 1039 1419 1.45e-46 176
Msa1132280 MtrunA17_Chr4g0064651 67.480 123 40 0 395 517 1 123 2.47e-45 156
Msa1132280 MtrunA17_Chr2g0293271 29.399 449 229 12 41 483 43 409 3.59e-40 151
Msa1132280 MtrunA17_Chr7g0245531 39.234 209 122 3 328 535 1 205 1.50e-39 146
Msa1132280 MtrunA17_Chr1g0151991 34.256 289 167 9 29 308 9 283 1.29e-38 143
Msa1132280 MtrunA17_Chr4g0057741 61.947 113 41 1 234 344 1 113 1.52e-38 137
Msa1132280 MtrunA17_Chr7g0245521 32.143 280 169 8 28 300 8 273 1.71e-34 131
Msa1132280 MtrunA17_Chr1g0166551 25.301 498 243 12 29 521 5 378 5.79e-34 135
Msa1132280 MtrunA17_Chr1g0152001 38.021 192 112 5 340 530 2 187 9.26e-33 128
Msa1132280 MtrunA17_Chr4g0057751 53.846 104 48 0 118 221 1 104 5.14e-29 110
Msa1132280 MtrunA17_Chr1g0188611 36.216 185 113 3 348 531 3 183 2.02e-27 112
Msa1132280 MtrunA17_Chr4g0004331 25.105 474 316 11 30 476 4 465 7.06e-27 115
Msa1132280 MtrunA17_Chr3g0098761 25.571 438 292 13 30 455 4 419 2.68e-25 110
Msa1132280 MtrunA17_Chr7g0223761 33.161 193 117 6 270 456 7 193 2.99e-25 107
Msa1132280 MtrunA17_Chr5g0394221 32.487 197 125 3 329 523 75 265 4.31e-24 103
Msa1132280 MtrunA17_Chr4g0064661 57.143 84 32 2 95 177 1 81 1.14e-23 95.1
Msa1132280 MtrunA17_Chr2g0331901 23.385 449 313 10 30 461 4 438 1.07e-22 102
Msa1132280 MtrunA17_Chr6g0462751 25.348 359 196 12 82 436 11 301 7.34e-22 96.7
Msa1132280 MtrunA17_Chr5g0444001 24.494 445 302 13 30 459 4 429 1.32e-21 98.6
Msa1132280 MtrunA17_Chr7g0223771 33.557 149 91 3 126 273 14 155 1.70e-21 91.7
Msa1132280 MtrunA17_Chr5g0442181 85.106 47 7 0 526 572 1 47 2.00e-21 87.4
Msa1132280 MtrunA17_Chr7g0223671 31.633 196 121 5 86 279 4 188 2.68e-21 92.4
Msa1132280 MtrunA17_Chr6g0462631 23.739 337 204 8 108 440 8 295 3.36e-21 94.4
Msa1132280 MtrunA17_Chr6g0462691 35.461 141 90 1 348 488 3 142 1.18e-18 83.2
Msa1132280 MtrunA17_Chr1g0188601 30.481 187 115 4 126 307 15 191 2.59e-18 83.6
Msa1132280 MtrunA17_Chr3g0120101 22.846 499 313 15 23 479 20 488 3.78e-18 88.6
Msa1132280 MtrunA17_Chr7g0245541 46.667 90 47 1 446 535 4 92 1.63e-16 78.6
Msa1132280 MtrunA17_Chr4g0046881 35.075 134 76 4 158 290 17 140 6.19e-16 75.5
Msa1132280 MtrunA17_Chr6g0462791 32.168 143 89 2 134 275 7 142 3.51e-15 73.9
Msa1132280 MtrunA17_Chr7g0223861 36.364 121 75 2 95 214 1 120 5.70e-15 72.0
Msa1132280 MtrunA17_Chr4g0046871 38.000 100 58 2 363 461 9 105 2.33e-12 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1132280 AT2G32120.1 73.684 570 136 2 5 571 3 561 0.0 895
Msa1132280 AT2G32120.2 73.684 570 136 2 5 571 3 561 0.0 895
Msa1132280 AT3G12580.1 38.020 505 286 11 29 523 9 496 6.94e-102 322
Msa1132280 AT5G02500.1 37.030 505 291 10 29 523 9 496 3.75e-99 315
Msa1132280 AT3G09440.1 36.750 517 276 11 29 523 9 496 6.82e-98 311
Msa1132280 AT3G09440.4 36.750 517 276 11 29 523 9 496 6.82e-98 311
Msa1132280 AT3G09440.2 36.750 517 276 11 29 523 9 496 6.82e-98 311
Msa1132280 AT3G09440.3 36.750 517 276 11 29 523 9 496 6.82e-98 311
Msa1132280 AT1G16030.1 36.047 516 285 10 27 523 6 495 1.21e-97 311
Msa1132280 AT1G56410.1 37.030 505 291 10 29 523 9 496 1.57e-94 301
Msa1132280 AT5G02490.1 35.842 505 297 10 29 523 9 496 4.60e-94 301
Msa1132280 AT1G09080.2 34.469 499 306 8 30 523 43 525 9.34e-86 280
Msa1132280 AT1G09080.1 34.469 499 306 8 30 523 53 535 1.60e-85 280
Msa1132280 AT5G42020.1 33.074 514 321 10 16 523 25 521 8.62e-84 275
Msa1132280 AT5G42020.3 33.074 514 321 10 16 523 30 526 1.25e-83 275
Msa1132280 AT5G42020.2 33.268 511 317 11 16 519 25 518 1.19e-82 270
Msa1132280 AT5G28540.1 33.600 500 310 9 30 523 38 521 1.26e-82 272
Msa1132280 AT5G09590.1 30.550 527 300 11 22 523 52 537 5.25e-67 230
Msa1132280 AT4G37910.1 30.755 530 295 12 22 523 47 532 4.57e-64 223
Msa1132280 AT4G37910.2 30.755 530 295 12 22 523 47 532 4.57e-64 223
Msa1132280 AT5G02500.2 34.097 393 239 8 29 414 9 388 9.59e-63 215
Msa1132280 AT5G49910.1 31.299 508 312 14 27 523 78 559 2.22e-60 213
Msa1132280 AT4G24280.1 31.434 509 310 15 27 523 78 559 4.63e-59 209
Msa1132280 AT1G11660.4 26.247 461 306 16 30 476 4 444 8.82e-27 115
Msa1132280 AT1G11660.3 26.247 461 306 16 30 476 4 444 8.82e-27 115
Msa1132280 AT1G11660.1 26.247 461 306 16 30 476 4 444 8.82e-27 115
Msa1132280 AT1G79930.2 24.944 449 306 10 30 461 4 438 4.30e-26 114
Msa1132280 AT1G79930.1 24.944 449 306 10 30 461 4 438 4.82e-26 113
Msa1132280 AT1G79920.1 24.499 449 308 10 30 461 4 438 7.69e-24 106
Msa1132280 AT1G79920.3 24.499 449 308 10 30 461 4 438 9.03e-24 106
Msa1132280 AT1G79920.2 24.499 449 308 10 30 461 4 438 9.03e-24 106
Msa1132280 AT1G79920.4 24.499 449 308 10 30 461 4 438 9.03e-24 106
Msa1132280 AT1G11660.2 27.000 400 260 15 91 476 26 407 2.74e-23 105
Msa1132280 AT4G16660.2 25.000 360 237 10 143 480 164 512 6.17e-19 91.7
Msa1132280 AT4G16660.1 25.000 360 237 10 143 480 140 488 8.45e-19 90.9

Find 176 sgRNAs with CRISPR-Local

Find 237 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTAGGATATTTCAAGATAA+TGG 0.182846 7_3:-85141715 Msa1132280:CDS
TGGCACCATAGGTCCTATTT+AGG 0.200225 7_3:+85141514 None:intergenic
AGAAGTCCTTGCAATGTTTC+TGG 0.201625 7_3:-85142750 Msa1132280:CDS
TCCATGCACACACTGATTTC+AGG 0.246998 7_3:+85141666 None:intergenic
TTCACTAACGCAGCAATAAA+TGG 0.261599 7_3:+85142800 None:intergenic
CAGTGATCCCTTTGGAAACT+TGG 0.262026 7_3:-85141934 Msa1132280:CDS
TCAATTCCAATCGCTATTTC+AGG 0.266817 7_3:+85143103 None:intergenic
GTGGTGTTCCTTGGAATTAC+AGG 0.282170 7_3:+85141846 None:intergenic
CTGATTTCAGGAACTCCTTT+TGG 0.283375 7_3:+85141678 None:intergenic
TTCAAAGACGAAGCTCCTTC+TGG 0.283594 7_3:-85142980 Msa1132280:CDS
GAAAGCTTCTCAAGAAACTA+TGG 0.285521 7_3:-85142537 Msa1132280:CDS
TGTTCAGGTTGATGTAGATT+TGG 0.316072 7_3:-85142243 Msa1132280:CDS
CTATGGTGCCACAATGGATT+TGG 0.318656 7_3:-85141502 Msa1132280:CDS
AACATTGCAAGGACTTCTTC+TGG 0.325894 7_3:+85142755 None:intergenic
AAATGTGGTGCTTACTGTTC+CGG 0.327314 7_3:-85142681 Msa1132280:CDS
TTAGGATATTTCAAGATAAT+GGG 0.332403 7_3:-85141714 Msa1132280:CDS
ACAGCAGTGGCGTTATTATA+TGG 0.334823 7_3:-85142572 Msa1132280:CDS
ATAGCGATTGGAATTGATAT+TGG 0.340874 7_3:-85143097 Msa1132280:CDS
TAACAGGCACCACAGGTATA+CGG 0.347738 7_3:+85141589 None:intergenic
AACTTCTGTTATTATGCATA+AGG 0.352864 7_3:+85142028 None:intergenic
GGCACCATAGGTCCTATTTA+GGG 0.356331 7_3:+85141515 None:intergenic
TACCATCGGTGGAGAAGATT+TGG 0.359082 7_3:-85142402 Msa1132280:CDS
ATGTAAATCAACTTCTATTC+TGG 0.362470 7_3:+85141411 None:intergenic
GTTCAGGTTGATGTAGATTT+GGG 0.369664 7_3:-85142242 Msa1132280:CDS
GGCATCCTCACTTCCATAAC+AGG 0.369992 7_3:+85141573 None:intergenic
AACGCCACTGCTGTTGGTTC+AGG 0.370548 7_3:+85142581 None:intergenic
ATCAACCTGAACACTGCTTT+GGG 0.377089 7_3:+85142253 None:intergenic
ATGGAAGGAGCTGTTGCTTC+AGG 0.378268 7_3:-85141960 Msa1132280:CDS
CATCAACCTGAACACTGCTT+TGG 0.383070 7_3:+85142252 None:intergenic
TTCCACCCAAAGCAGTGTTC+AGG 0.388302 7_3:-85142258 Msa1132280:CDS
GTAAGCACCACATTTCTTAT+CGG 0.392583 7_3:+85142689 None:intergenic
CACGACCATGAGATGCTGTT+TGG 0.393050 7_3:-85142935 Msa1132280:CDS
TCGCACAAGCACGCTCTAAA+CGG 0.395103 7_3:+85142633 None:intergenic
TATACCTGTGGTGCCTGTTA+TGG 0.395756 7_3:-85141586 Msa1132280:CDS
CATGTAAACATTGGATGATA+AGG 0.397163 7_3:+85142138 None:intergenic
TCCAAGGTCTGCACCAGAAA+TGG 0.397431 7_3:+85142836 None:intergenic
CAACTGAGGGGCAGTGTTAG+TGG 0.400912 7_3:+85141267 None:intergenic
GCAGAAGAAAACCACTTGTT+AGG 0.403341 7_3:-85141732 Msa1132280:CDS
TGGCTGGTCTTCATGTTCTC+AGG 0.414478 7_3:-85142610 Msa1132280:CDS
TTATAAAGGTATAAACCCTT+TGG 0.418915 7_3:-85142006 Msa1132280:CDS
TGGAAACGACTGAACGAAAC+CGG 0.426040 7_3:+85142662 None:intergenic
CATGATGCAAAGGTAGAAGT+TGG 0.434870 7_3:-85142119 Msa1132280:CDS
CCTTTGGAAGGTGCGGTTTG+TGG 0.435575 7_3:-85141990 Msa1132280:CDS
CACGCTCTAAACGGGTCAGC+TGG 0.440361 7_3:+85142642 None:intergenic
ATAATAACAGAAGTTTATAA+AGG 0.443225 7_3:-85142020 Msa1132280:CDS
GGTGTGCTGAGGCCCTAAAT+AGG 0.444919 7_3:-85141527 Msa1132280:CDS
GCCATTTCTGGTGCAGACCT+TGG 0.447888 7_3:-85142837 Msa1132280:CDS
ATGATGCAAAGGTAGAAGTT+GGG 0.448859 7_3:-85142118 Msa1132280:CDS
GCTGGCATTGTGGTGTTCCT+TGG 0.449119 7_3:+85141837 None:intergenic
GCATCTGACAGTGAAACAAC+TGG 0.449771 7_3:-85143148 Msa1132280:CDS
ATCAATTTATGCCTTCTTGA+GGG 0.450343 7_3:+85141476 None:intergenic
CAGAAGGAAATTTAAACATC+AGG 0.453605 7_3:+85141313 None:intergenic
CTGCGTTAGTGAACAATGTC+TGG 0.455549 7_3:-85142790 Msa1132280:CDS
TCAGGCATCAGTGATCCCTT+TGG 0.456587 7_3:-85141942 Msa1132280:CDS
TGTTGACAGGGCGGAGTTTG+AGG 0.457797 7_3:-85142195 Msa1132280:CDS
CTGGTGCAGACCTTGGACAT+TGG 0.457903 7_3:-85142830 Msa1132280:CDS
GCCATTGGAATTCGAGCTGA+TGG 0.458770 7_3:-85141882 Msa1132280:CDS
AAAATATATTCAAGCGTCAT+GGG 0.460640 7_3:-85142343 Msa1132280:CDS
ATAAATGATGTGATACTTGT+TGG 0.460986 7_3:-85142092 Msa1132280:CDS
CGCACAAGCACGCTCTAAAC+GGG 0.463935 7_3:+85142634 None:intergenic
GTAACAAGATTCTCCACCCT+TGG 0.466538 7_3:+85142053 None:intergenic
GAGCGTGCTTGTGCGATGGC+TGG 0.467592 7_3:-85142626 Msa1132280:CDS
AATATGAGAGAGAAAACAAA+CGG 0.470264 7_3:+85144305 None:intergenic
TTAGCCGGTGTTGTGATGCC+TGG 0.471501 7_3:-85141615 Msa1132280:CDS
CAAGCCACTCCTCTTGCCAT+TGG 0.482744 7_3:-85141897 Msa1132280:CDS
CCAATCGCTATTTCAGGAAG+AGG 0.484412 7_3:+85143109 None:intergenic
GTTGATGTAGATTTGGGAGA+TGG 0.485617 7_3:-85142236 Msa1132280:CDS
TATAATAACGCCACTGCTGT+TGG 0.489694 7_3:+85142575 None:intergenic
GGTCAAAGAAATTAAATCAA+TGG 0.491607 7_3:-85142321 Msa1132280:CDS
TAACAAGATTCTCCACCCTT+GGG 0.492275 7_3:+85142054 None:intergenic
GGGTTAACAAATAAACCAAC+TGG 0.494649 7_3:+85141288 None:intergenic
GAAAATATATTCAAGCGTCA+TGG 0.496398 7_3:-85142344 Msa1132280:CDS
CAAATCCATTGTGGCACCAT+AGG 0.497810 7_3:+85141503 None:intergenic
AAAGCTGATGAATTGCATCC+TGG 0.503417 7_3:+85142279 None:intergenic
AAATTTCCTTCTGTACCAGT+TGG 0.505683 7_3:-85141303 Msa1132280:three_prime_UTR
CCTCTTCCTGAAATAGCGAT+TGG 0.506297 7_3:-85143109 Msa1132280:CDS
GTTGCTGTGACTGCTACCGC+CGG 0.506470 7_3:-85142458 Msa1132280:CDS
TTGCTGTGACTGCTACCGCC+GGG 0.511136 7_3:-85142457 Msa1132280:CDS
AACAAGTCCAAGTTTCCAAA+GGG 0.511681 7_3:+85141927 None:intergenic
AGATATGCAAGGTTGTTGAC+AGG 0.512232 7_3:-85142208 Msa1132280:CDS
TATCAATTTATGCCTTCTTG+AGG 0.516603 7_3:+85141475 None:intergenic
AACAGGCACCACAGGTATAC+GGG 0.517047 7_3:+85141590 None:intergenic
GGAATACCTGCAGCACCAAA+AGG 0.524506 7_3:-85141693 Msa1132280:CDS
CAAACAGCATCTCATGGTCG+TGG 0.528773 7_3:+85142936 None:intergenic
CACAAACCGCACCTTCCAAA+GGG 0.529952 7_3:+85141991 None:intergenic
TTCAATATGAAACGCTTGAT+CGG 0.530493 7_3:-85142902 Msa1132280:CDS
CCTTTGCATCATGTAAACAT+TGG 0.531721 7_3:+85142129 None:intergenic
GAGGCCCTAAATAGGACCTA+TGG 0.533257 7_3:-85141519 Msa1132280:CDS
TGCGGTTTGTGGTGCAGCAA+TGG 0.533347 7_3:-85141979 Msa1132280:CDS
ACCACAGGTATACGGGATCC+AGG 0.535468 7_3:+85141597 None:intergenic
TCCTGAAATCAGTGTGTGCA+TGG 0.540172 7_3:-85141667 Msa1132280:CDS
TCTTGAAATATCCTAACAAG+TGG 0.545629 7_3:+85141721 None:intergenic
AAAAGTAGATCTTTACGTGC+TGG 0.548488 7_3:+85141819 None:intergenic
AACTGAGGGGCAGTGTTAGT+GGG 0.551291 7_3:+85141268 None:intergenic
AACCTGAACACTGCTTTGGG+TGG 0.551609 7_3:+85142256 None:intergenic
AAGGACTTCTTCTGGAGTGG+TGG 0.559811 7_3:+85142763 None:intergenic
TGCAAGGACTTCTTCTGGAG+TGG 0.559852 7_3:+85142760 None:intergenic
TCCCAGATAAAAGCATTGGC+AGG 0.560848 7_3:-85142428 Msa1132280:CDS
TCTCAAGAAACTATGGGCAG+TGG 0.563843 7_3:-85142530 Msa1132280:CDS
GGATTTGGTTACCCTCAAGA+AGG 0.565408 7_3:-85141487 Msa1132280:CDS
ATATGAGAGAGAAAACAAAC+GGG 0.566282 7_3:+85144306 None:intergenic
AACCAAATCTTCTCCACCGA+TGG 0.568389 7_3:+85142400 None:intergenic
TGGACGAACGCCAATGTCCA+AGG 0.570950 7_3:+85142820 None:intergenic
GTACGAAGGTGAGGGAAAGA+AGG 0.571023 7_3:-85141754 Msa1132280:CDS
TTTAGAGCGTGCTTGTGCGA+TGG 0.572607 7_3:-85142630 Msa1132280:CDS
AATAAACCAACTGGTACAGA+AGG 0.572912 7_3:+85141297 None:intergenic
TCAGATGCAACGGTATATGC+AGG 0.578112 7_3:+85143163 None:intergenic
GATACTTGTGTACGAAGGTG+AGG 0.578994 7_3:-85141763 Msa1132280:CDS
AAAGGTATAAACCCTTTGGA+AGG 0.581870 7_3:-85142002 Msa1132280:CDS
TGTTTCACTGTCAGATGCAA+CGG 0.582470 7_3:+85143153 None:intergenic
CCCATCAGCTCGAATTCCAA+TGG 0.583989 7_3:+85141881 None:intergenic
TAACAAGTCCAAGTTTCCAA+AGG 0.584586 7_3:+85141926 None:intergenic
GAGGATGTTCTTATATCCCA+AGG 0.584781 7_3:-85142070 Msa1132280:CDS
CCATGACCATCATCAACCAT+TGG 0.585720 7_3:+85141552 None:intergenic
AGTCCTTGCAATGTTTCTGG+TGG 0.586924 7_3:-85142747 Msa1132280:CDS
AAGCTGATGAATTGCATCCT+GGG 0.587754 7_3:+85142280 None:intergenic
AGTATCCCAGATAAAAGCAT+TGG 0.589832 7_3:-85142432 Msa1132280:CDS
ATTGCTTCGAGTTGCAACCC+AGG 0.590200 7_3:-85142297 Msa1132280:CDS
CCACAAACCGCACCTTCCAA+AGG 0.591096 7_3:+85141990 None:intergenic
TGTAGTGTTGCTGTGTGGAA+TGG 0.591608 7_3:-85143064 Msa1132280:CDS
TGATGCAAAGGTAGAAGTTG+GGG 0.591780 7_3:-85142117 Msa1132280:CDS
ACTTCCATAACAGGCACCAC+AGG 0.592348 7_3:+85141582 None:intergenic
CCAATGTTTACATGATGCAA+AGG 0.592560 7_3:-85142129 Msa1132280:CDS
TGCTGTGACTGCTACCGCCG+GGG 0.595836 7_3:-85142456 Msa1132280:CDS
CACAGCAACACTACATTGTG+AGG 0.598044 7_3:+85143072 None:intergenic
CATCTGACAGTGAAACAACT+GGG 0.598563 7_3:-85143147 Msa1132280:CDS
ATGGTCGTGGGAGAATTGAC+TGG 0.601519 7_3:+85142949 None:intergenic
CCATTGGAATTCGAGCTGAT+GGG 0.601553 7_3:-85141881 Msa1132280:CDS
AGTTTGTACCTGTAATTCCA+AGG 0.609145 7_3:-85141854 Msa1132280:CDS
AAAGACGAAGCTCCTTCTGG+AGG 0.611357 7_3:-85142977 Msa1132280:CDS
ATCTTTACGTGCTGGCATTG+TGG 0.613475 7_3:+85141827 None:intergenic
AATTCCAATGGCAAGAGGAG+TGG 0.613750 7_3:+85141893 None:intergenic
AAACAGCATCTCATGGTCGT+GGG 0.616728 7_3:+85142937 None:intergenic
TATCTCCAAACAGCATCTCA+TGG 0.617418 7_3:+85142930 None:intergenic
GTTTGTGGTGCAGCAATGGA+AGG 0.619096 7_3:-85141975 Msa1132280:CDS
TGGTCATGGTTGGTGTGCTG+AGG 0.620833 7_3:-85141538 Msa1132280:CDS
AAAGCTTCTCAAGAAACTAT+GGG 0.624225 7_3:-85142536 Msa1132280:CDS
AATGATGTGATACTTGTTGG+AGG 0.629718 7_3:-85142089 Msa1132280:CDS
TAGGACCTATGGTGCCACAA+TGG 0.630018 7_3:-85141508 Msa1132280:CDS
ACTCACTCACTCACTCACTC+AGG 0.631246 7_3:-85144236 Msa1132280:intron
TGGTGCCTGTTATGGAAGTG+AGG 0.631903 7_3:-85141578 Msa1132280:CDS
TGACAGGGCGGAGTTTGAGG+AGG 0.633647 7_3:-85142192 Msa1132280:CDS
TATAAACCCTTTGGAAGGTG+CGG 0.634750 7_3:-85141997 Msa1132280:CDS
GCATTGGCAGGAAGTACCAT+CGG 0.637875 7_3:-85142416 Msa1132280:CDS
AGGATGCCAATGGTTGATGA+TGG 0.639984 7_3:-85141558 Msa1132280:CDS
GGATCCAGGCATCACAACAC+CGG 0.644727 7_3:+85141611 None:intergenic
GATATGCAAGGTTGTTGACA+GGG 0.644837 7_3:-85142207 Msa1132280:CDS
ATACTTGTGTACGAAGGTGA+GGG 0.646011 7_3:-85141762 Msa1132280:CDS
AGGATGTTCTTATATCCCAA+GGG 0.646556 7_3:-85142069 Msa1132280:CDS
GTTTGAGGAGGTGAACAAAG+AGG 0.649304 7_3:-85142180 Msa1132280:CDS
GCTCGAATTCCAATGGCAAG+AGG 0.649760 7_3:+85141888 None:intergenic
TGGTTGATGATGGTCATGGT+TGG 0.651359 7_3:-85141548 Msa1132280:CDS
TCAGGTATGCACAGACGAGG+TGG 0.652682 7_3:+85141331 None:intergenic
CAAACGACCGATAAGAAATG+TGG 0.653357 7_3:-85142696 Msa1132280:CDS
TGACAGTGAAACAACTGGGG+AGG 0.653527 7_3:-85143143 Msa1132280:CDS
ACTCCACCAGAAACATTGCA+AGG 0.653765 7_3:+85142744 None:intergenic
GCCTGGATCCCGTATACCTG+TGG 0.656774 7_3:-85141598 Msa1132280:CDS
CCAATGGTTGATGATGGTCA+TGG 0.660629 7_3:-85141552 Msa1132280:CDS
ATGTTCTTATATCCCAAGGG+TGG 0.662483 7_3:-85142066 Msa1132280:CDS
TGACTGGTAACTCCTCCAGA+AGG 0.672347 7_3:+85142965 None:intergenic
GAGGGTAACCAAATCCATTG+TGG 0.675212 7_3:+85141494 None:intergenic
GATGCCTGAACCAACAGCAG+TGG 0.675835 7_3:-85142585 Msa1132280:CDS
CAGAGTTGATACTGACCCTG+TGG 0.678557 7_3:-85142879 Msa1132280:CDS
TATTCATGATAATCAAACTG+AGG 0.679559 7_3:-85141790 Msa1132280:CDS
GCTGTGACTGCTACCGCCGG+GGG 0.681642 7_3:-85142455 Msa1132280:CDS
AAATATATTCAAGCGTCATG+GGG 0.682975 7_3:-85142342 Msa1132280:CDS
CACAATGTAGTGTTGCTGTG+TGG 0.686328 7_3:-85143069 Msa1132280:CDS
AGATGGTTTGAAGATATGCA+AGG 0.687926 7_3:-85142219 Msa1132280:CDS
TTGGCAGGAAGTACCATCGG+TGG 0.690515 7_3:-85142413 Msa1132280:CDS
ATGCAAGGTTGTTGACAGGG+CGG 0.708122 7_3:-85142204 Msa1132280:CDS
ACATCAGGTATGCACAGACG+AGG 0.716596 7_3:+85141328 None:intergenic
TGTGTGGAATGGCTCCGAAG+TGG 0.718800 7_3:-85143053 Msa1132280:CDS
TATGGAAGTGAGGATGCCAA+TGG 0.723950 7_3:-85141568 Msa1132280:CDS
TGAGTGAGTGAGTGAGCGAG+CGG 0.729719 7_3:+85144246 None:intergenic
ATCTGACAGTGAAACAACTG+GGG 0.741112 7_3:-85143146 Msa1132280:CDS
GCATTGATACTTGTGTACGA+AGG 0.792141 7_3:-85141768 Msa1132280:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAAAAAGATAATAACTAAA+TGG + chr7_3:85141489-85141508 None:intergenic 10.0%
!!! TCTTCTTTTTTATTTTATTA+TGG - chr7_3:85141570-85141589 Msa1132280:CDS 10.0%
!! TTAAAACATTAAAAAAATCA+GGG + chr7_3:85141676-85141695 None:intergenic 10.0%
!!! TTTAAAACATTAAAAAAATC+AGG + chr7_3:85141677-85141696 None:intergenic 10.0%
!!! ATTGTAACTTTTAAAATATA+GGG - chr7_3:85141809-85141828 Msa1132280:CDS 10.0%
!! TTTAAAAGTTACAATCAATT+TGG + chr7_3:85141804-85141823 None:intergenic 15.0%
!!! GATTGTAACTTTTAAAATAT+AGG - chr7_3:85141808-85141827 Msa1132280:CDS 15.0%
!! AGATTATAAGAAATTCAAAA+AGG + chr7_3:85141898-85141917 None:intergenic 15.0%
!! ATAATAACAGAAGTTTATAA+AGG - chr7_3:85143567-85143586 Msa1132280:intron 15.0%
!! TTGAATTTCTTATAATCTCA+AGG - chr7_3:85141900-85141919 Msa1132280:CDS 20.0%
!! AAATTTGAGTATGTAATGTT+TGG + chr7_3:85142113-85142132 None:intergenic 20.0%
!! ATGTTAATATTAAGCACTAT+TGG + chr7_3:85142226-85142245 None:intergenic 20.0%
!! TTCAGATATAATTACATACA+TGG + chr7_3:85142269-85142288 None:intergenic 20.0%
!!! TTGAATATATTTTCAGAATC+TGG + chr7_3:85143237-85143256 None:intergenic 20.0%
!! TTAGGATATTTCAAGATAAT+GGG - chr7_3:85143873-85143892 Msa1132280:intron 20.0%
! AATATGAGAGAGAAAACAAA+CGG + chr7_3:85141285-85141304 None:intergenic 25.0%
! TTTAAATCAACTTGCCTTAT+AGG - chr7_3:85141505-85141524 Msa1132280:CDS 25.0%
! TCAAATTCAAACAATATGCT+AGG - chr7_3:85141739-85141758 Msa1132280:CDS 25.0%
! CAAATTCAAACAATATGCTA+GGG - chr7_3:85141740-85141759 Msa1132280:CDS 25.0%
!!! TTGACAAGTTTAAAAGTGTT+AGG - chr7_3:85141870-85141889 Msa1132280:CDS 25.0%
!!! TGACAAGTTTAAAAGTGTTA+GGG - chr7_3:85141871-85141890 Msa1132280:CDS 25.0%
! ATGTTACTGTGTATAATTTG+TGG - chr7_3:85141994-85142013 Msa1132280:CDS 25.0%
!! TGTGTATAATTTGTGGTATT+TGG - chr7_3:85142001-85142020 Msa1132280:CDS 25.0%
! ACAGTTGTTAAAACAATTAC+AGG + chr7_3:85142071-85142090 None:intergenic 25.0%
!!! TGTTTTAACAACTGTGAAAA+TGG - chr7_3:85142076-85142095 Msa1132280:CDS 25.0%
! GCTTTCTATATTAGATTGTT+CGG - chr7_3:85142352-85142371 Msa1132280:CDS 25.0%
!! AATTGCTCTCATTTTCAATA+TGG - chr7_3:85143089-85143108 Msa1132280:CDS 25.0%
!! ATTGCTCTCATTTTCAATAT+GGG - chr7_3:85143090-85143109 Msa1132280:CDS 25.0%
! AAAATATATTCAAGCGTCAT+GGG - chr7_3:85143244-85143263 Msa1132280:intron 25.0%
! GGTCAAAGAAATTAAATCAA+TGG - chr7_3:85143266-85143285 Msa1132280:intron 25.0%
! ATAAATGATGTGATACTTGT+TGG - chr7_3:85143495-85143514 Msa1132280:intron 25.0%
! AACTTCTGTTATTATGCATA+AGG + chr7_3:85143562-85143581 None:intergenic 25.0%
!! TTATAAAGGTATAAACCCTT+TGG - chr7_3:85143581-85143600 Msa1132280:intron 25.0%
! TATTCATGATAATCAAACTG+AGG - chr7_3:85143797-85143816 Msa1132280:intron 25.0%
! GTTAGGATATTTCAAGATAA+TGG - chr7_3:85143872-85143891 Msa1132280:intron 25.0%
! CACAGAAAAACAACTAAAAT+AGG + chr7_3:85144154-85144173 None:intergenic 25.0%
! ATGTAAATCAACTTCTATTC+TGG + chr7_3:85144179-85144198 None:intergenic 25.0%
ATATGAGAGAGAAAACAAAC+GGG + chr7_3:85141284-85141303 None:intergenic 30.0%
TAATAACTAAATGGCACTGT+TGG + chr7_3:85141480-85141499 None:intergenic 30.0%
AGTAGTTACAAACACCTATA+AGG + chr7_3:85141522-85141541 None:intergenic 30.0%
!! TGATGATGGATCATAAACTT+AGG - chr7_3:85141770-85141789 Msa1132280:CDS 30.0%
!! GATGATGGATCATAAACTTA+GGG - chr7_3:85141771-85141790 Msa1132280:CDS 30.0%
! CACTTTTAAACTTGTCAAGT+TGG + chr7_3:85141868-85141887 None:intergenic 30.0%
ATTTGGTCAGATATGTACTT+TGG - chr7_3:85142018-85142037 Msa1132280:CDS 30.0%
TTTAACAACTGTGAAAATGG+AGG - chr7_3:85142079-85142098 Msa1132280:CDS 30.0%
! CAATTTTTCCTGTTAAATGC+AGG - chr7_3:85142386-85142405 Msa1132280:CDS 30.0%
ATAGCGATTGGAATTGATAT+TGG - chr7_3:85142490-85142509 Msa1132280:CDS 30.0%
TTCAATATGAAACGCTTGAT+CGG - chr7_3:85142685-85142704 Msa1132280:CDS 30.0%
! AAGTAAAAATCTGCCATTTC+TGG - chr7_3:85142738-85142757 Msa1132280:CDS 30.0%
AAAGCTTCTCAAGAAACTAT+GGG - chr7_3:85143051-85143070 Msa1132280:CDS 30.0%
GAAAATATATTCAAGCGTCA+TGG - chr7_3:85143243-85143262 Msa1132280:intron 30.0%
AAATATATTCAAGCGTCATG+GGG - chr7_3:85143245-85143264 Msa1132280:intron 30.0%
CATGTAAACATTGGATGATA+AGG + chr7_3:85143452-85143471 None:intergenic 30.0%
TCTTGAAATATCCTAACAAG+TGG + chr7_3:85143869-85143888 None:intergenic 30.0%
ATCAATTTATGCCTTCTTGA+GGG + chr7_3:85144114-85144133 None:intergenic 30.0%
TATCAATTTATGCCTTCTTG+AGG + chr7_3:85144115-85144134 None:intergenic 30.0%
CAGAAGGAAATTTAAACATC+AGG + chr7_3:85144277-85144296 None:intergenic 30.0%
CATGCATGACACTTTCAATT+TGG - chr7_3:85141649-85141668 Msa1132280:CDS 35.0%
TGGATCATAAACTTAGGGTT+AGG - chr7_3:85141776-85141795 Msa1132280:CDS 35.0%
GGATCATAAACTTAGGGTTA+GGG - chr7_3:85141777-85141796 Msa1132280:CDS 35.0%
! GGGACTAAATTGATAGTAAG+TGG - chr7_3:85141829-85141848 Msa1132280:CDS 35.0%
AATCCCTGACTTTCTCAAAT+AGG + chr7_3:85141927-85141946 None:intergenic 35.0%
GCTGCATGTAATTATATCTG+AGG - chr7_3:85142291-85142310 Msa1132280:CDS 35.0%
! TTTTCCTGTTAAATGCAGGA+TGG - chr7_3:85142390-85142409 Msa1132280:CDS 35.0%
! TCAATTCCAATCGCTATTTC+AGG + chr7_3:85142487-85142506 None:intergenic 35.0%
!! GTTTTTCAAAAGCTCCACTT+CGG + chr7_3:85142551-85142570 None:intergenic 35.0%
! TTTTACTTGCATGAACCACA+GGG + chr7_3:85142726-85142745 None:intergenic 35.0%
! TTTTTACTTGCATGAACCAC+AGG + chr7_3:85142727-85142746 None:intergenic 35.0%
TTCACTAACGCAGCAATAAA+TGG + chr7_3:85142790-85142809 None:intergenic 35.0%
GTAAGCACCACATTTCTTAT+CGG + chr7_3:85142901-85142920 None:intergenic 35.0%
GAAAGCTTCTCAAGAAACTA+TGG - chr7_3:85143050-85143069 Msa1132280:CDS 35.0%
! AGTATCCCAGATAAAAGCAT+TGG - chr7_3:85143155-85143174 Msa1132280:CDS 35.0%
!! TGTTCAGGTTGATGTAGATT+TGG - chr7_3:85143344-85143363 Msa1132280:intron 35.0%
!! GTTCAGGTTGATGTAGATTT+GGG - chr7_3:85143345-85143364 Msa1132280:intron 35.0%
AGATGGTTTGAAGATATGCA+AGG - chr7_3:85143368-85143387 Msa1132280:intron 35.0%
CCTTTGCATCATGTAAACAT+TGG + chr7_3:85143461-85143480 None:intergenic 35.0%
CCAATGTTTACATGATGCAA+AGG - chr7_3:85143458-85143477 Msa1132280:intron 35.0%
ATGATGCAAAGGTAGAAGTT+GGG - chr7_3:85143469-85143488 Msa1132280:intron 35.0%
! AATGATGTGATACTTGTTGG+AGG - chr7_3:85143498-85143517 Msa1132280:intron 35.0%
AGGATGTTCTTATATCCCAA+GGG - chr7_3:85143518-85143537 Msa1132280:intron 35.0%
! AAAGGTATAAACCCTTTGGA+AGG - chr7_3:85143585-85143604 Msa1132280:intron 35.0%
AACAAGTCCAAGTTTCCAAA+GGG + chr7_3:85143663-85143682 None:intergenic 35.0%
TAACAAGTCCAAGTTTCCAA+AGG + chr7_3:85143664-85143683 None:intergenic 35.0%
AGTTTGTACCTGTAATTCCA+AGG - chr7_3:85143733-85143752 Msa1132280:intron 35.0%
AAAAGTAGATCTTTACGTGC+TGG + chr7_3:85143771-85143790 None:intergenic 35.0%
AAATTTCCTTCTGTACCAGT+TGG - chr7_3:85144284-85144303 Msa1132280:five_prime_UTR 35.0%
AATAAACCAACTGGTACAGA+AGG + chr7_3:85144293-85144312 None:intergenic 35.0%
GGGTTAACAAATAAACCAAC+TGG + chr7_3:85144302-85144321 None:intergenic 35.0%
ACAAGAGTAGCAGAAGAAGA+AGG + chr7_3:85141390-85141409 None:intergenic 40.0%
AACTAAATGGCACTGTTGGT+TGG + chr7_3:85141476-85141495 None:intergenic 40.0%
TGACATTTGATTGATGCAGC+AGG + chr7_3:85141545-85141564 None:intergenic 40.0%
GCTAGGGACTAAATTGATGA+TGG - chr7_3:85141756-85141775 Msa1132280:CDS 40.0%
AGGACCTATTTGAGAAAGTC+AGG - chr7_3:85141920-85141939 Msa1132280:CDS 40.0%
GGACCTATTTGAGAAAGTCA+GGG - chr7_3:85141921-85141940 Msa1132280:CDS 40.0%
GAGAAAGTCAGGGATTATGT+TGG - chr7_3:85141931-85141950 Msa1132280:CDS 40.0%
!! CTTATGAACAGAGCACTGTA+AGG + chr7_3:85142163-85142182 None:intergenic 40.0%
CTTACAGTGCTCTGTTCATA+AGG - chr7_3:85142161-85142180 Msa1132280:CDS 40.0%
TGTTTCACTGTCAGATGCAA+CGG + chr7_3:85142437-85142456 None:intergenic 40.0%
CATCTGACAGTGAAACAACT+GGG - chr7_3:85142440-85142459 Msa1132280:CDS 40.0%
ATCTGACAGTGAAACAACTG+GGG - chr7_3:85142441-85142460 Msa1132280:CDS 40.0%
TATCTCCAAACAGCATCTCA+TGG + chr7_3:85142660-85142679 None:intergenic 40.0%
AACATTGCAAGGACTTCTTC+TGG + chr7_3:85142835-85142854 None:intergenic 40.0%
AGAAGTCCTTGCAATGTTTC+TGG - chr7_3:85142837-85142856 Msa1132280:CDS 40.0%
CAAACGACCGATAAGAAATG+TGG - chr7_3:85142891-85142910 Msa1132280:CDS 40.0%
AAATGTGGTGCTTACTGTTC+CGG - chr7_3:85142906-85142925 Msa1132280:CDS 40.0%
TATAATAACGCCACTGCTGT+TGG + chr7_3:85143015-85143034 None:intergenic 40.0%
ACAGCAGTGGCGTTATTATA+TGG - chr7_3:85143015-85143034 Msa1132280:CDS 40.0%
! CTCATTTTCAATATGGGTGC+TGG - chr7_3:85143096-85143115 Msa1132280:CDS 40.0%
! TCCTGCCAATGCTTTTATCT+GGG + chr7_3:85143163-85143182 None:intergenic 40.0%
! TTCCTGCCAATGCTTTTATC+TGG + chr7_3:85143164-85143183 None:intergenic 40.0%
AAGCTGATGAATTGCATCCT+GGG + chr7_3:85143310-85143329 None:intergenic 40.0%
AAAGCTGATGAATTGCATCC+TGG + chr7_3:85143311-85143330 None:intergenic 40.0%
ATCAACCTGAACACTGCTTT+GGG + chr7_3:85143337-85143356 None:intergenic 40.0%
GTTGATGTAGATTTGGGAGA+TGG - chr7_3:85143351-85143370 Msa1132280:intron 40.0%
AGATATGCAAGGTTGTTGAC+AGG - chr7_3:85143379-85143398 Msa1132280:intron 40.0%
GATATGCAAGGTTGTTGACA+GGG - chr7_3:85143380-85143399 Msa1132280:intron 40.0%
CATGATGCAAAGGTAGAAGT+TGG - chr7_3:85143468-85143487 Msa1132280:intron 40.0%
TGATGCAAAGGTAGAAGTTG+GGG - chr7_3:85143470-85143489 Msa1132280:intron 40.0%
GAGGATGTTCTTATATCCCA+AGG - chr7_3:85143517-85143536 Msa1132280:intron 40.0%
ATGTTCTTATATCCCAAGGG+TGG - chr7_3:85143521-85143540 Msa1132280:intron 40.0%
TAACAAGATTCTCCACCCTT+GGG + chr7_3:85143536-85143555 None:intergenic 40.0%
! TATAAACCCTTTGGAAGGTG+CGG - chr7_3:85143590-85143609 Msa1132280:intron 40.0%
! GCATTGATACTTGTGTACGA+AGG - chr7_3:85143819-85143838 Msa1132280:intron 40.0%
ATACTTGTGTACGAAGGTGA+GGG - chr7_3:85143825-85143844 Msa1132280:intron 40.0%
GCAGAAGAAAACCACTTGTT+AGG - chr7_3:85143855-85143874 Msa1132280:intron 40.0%
! CTGATTTCAGGAACTCCTTT+TGG + chr7_3:85143912-85143931 None:intergenic 40.0%
TCTGCCATCCTGCATTTAAC+AGG + chr7_3:85142397-85142416 None:intergenic 45.0%
TCAGATGCAACGGTATATGC+AGG + chr7_3:85142427-85142446 None:intergenic 45.0%
GCATCTGACAGTGAAACAAC+TGG - chr7_3:85142439-85142458 Msa1132280:CDS 45.0%
! CCAATCGCTATTTCAGGAAG+AGG + chr7_3:85142481-85142500 None:intergenic 45.0%
CCTCTTCCTGAAATAGCGAT+TGG - chr7_3:85142478-85142497 Msa1132280:CDS 45.0%
CACAGCAACACTACATTGTG+AGG + chr7_3:85142518-85142537 None:intergenic 45.0%
! CACAATGTAGTGTTGCTGTG+TGG - chr7_3:85142518-85142537 Msa1132280:CDS 45.0%
! TGTAGTGTTGCTGTGTGGAA+TGG - chr7_3:85142523-85142542 Msa1132280:CDS 45.0%
TTCAAAGACGAAGCTCCTTC+TGG - chr7_3:85142607-85142626 Msa1132280:CDS 45.0%
AAACAGCATCTCATGGTCGT+GGG + chr7_3:85142653-85142672 None:intergenic 45.0%
CTGCGTTAGTGAACAATGTC+TGG - chr7_3:85142797-85142816 Msa1132280:CDS 45.0%
!! AGTCCTTGCAATGTTTCTGG+TGG - chr7_3:85142840-85142859 Msa1132280:CDS 45.0%
ACTCCACCAGAAACATTGCA+AGG + chr7_3:85142846-85142865 None:intergenic 45.0%
TGGAAACGACTGAACGAAAC+CGG + chr7_3:85142928-85142947 None:intergenic 45.0%
TCTCAAGAAACTATGGGCAG+TGG - chr7_3:85143057-85143076 Msa1132280:CDS 45.0%
!! TTTTATCTGGGATACTCCCC+CGG + chr7_3:85143151-85143170 None:intergenic 45.0%
!! TCCCAGATAAAAGCATTGGC+AGG - chr7_3:85143159-85143178 Msa1132280:CDS 45.0%
TACCATCGGTGGAGAAGATT+TGG - chr7_3:85143185-85143204 Msa1132280:intron 45.0%
AACCAAATCTTCTCCACCGA+TGG + chr7_3:85143190-85143209 None:intergenic 45.0%
CATCAACCTGAACACTGCTT+TGG + chr7_3:85143338-85143357 None:intergenic 45.0%
GTTTGAGGAGGTGAACAAAG+AGG - chr7_3:85143407-85143426 Msa1132280:intron 45.0%
GTAACAAGATTCTCCACCCT+TGG + chr7_3:85143537-85143556 None:intergenic 45.0%
! CAGTGATCCCTTTGGAAACT+TGG - chr7_3:85143653-85143672 Msa1132280:intron 45.0%
AATTCCAATGGCAAGAGGAG+TGG + chr7_3:85143697-85143716 None:intergenic 45.0%
CCATTGGAATTCGAGCTGAT+GGG - chr7_3:85143706-85143725 Msa1132280:intron 45.0%
GTGGTGTTCCTTGGAATTAC+AGG + chr7_3:85143744-85143763 None:intergenic 45.0%
! ATCTTTACGTGCTGGCATTG+TGG + chr7_3:85143763-85143782 None:intergenic 45.0%
GATACTTGTGTACGAAGGTG+AGG - chr7_3:85143824-85143843 Msa1132280:intron 45.0%
TCCTGAAATCAGTGTGTGCA+TGG - chr7_3:85143920-85143939 Msa1132280:intron 45.0%
!! TCCATGCACACACTGATTTC+AGG + chr7_3:85143924-85143943 None:intergenic 45.0%
TAACAGGCACCACAGGTATA+CGG + chr7_3:85144001-85144020 None:intergenic 45.0%
TATACCTGTGGTGCCTGTTA+TGG - chr7_3:85144001-85144020 Msa1132280:intron 45.0%
TATGGAAGTGAGGATGCCAA+TGG - chr7_3:85144019-85144038 Msa1132280:intron 45.0%
!! AGGATGCCAATGGTTGATGA+TGG - chr7_3:85144029-85144048 Msa1132280:intron 45.0%
CCATGACCATCATCAACCAT+TGG + chr7_3:85144038-85144057 None:intergenic 45.0%
!! CCAATGGTTGATGATGGTCA+TGG - chr7_3:85144035-85144054 Msa1132280:intron 45.0%
!! TGGTTGATGATGGTCATGGT+TGG - chr7_3:85144039-85144058 Msa1132280:intron 45.0%
GGCACCATAGGTCCTATTTA+GGG + chr7_3:85144075-85144094 None:intergenic 45.0%
TGGCACCATAGGTCCTATTT+AGG + chr7_3:85144076-85144095 None:intergenic 45.0%
CAAATCCATTGTGGCACCAT+AGG + chr7_3:85144087-85144106 None:intergenic 45.0%
!! CTATGGTGCCACAATGGATT+TGG - chr7_3:85144085-85144104 Msa1132280:intron 45.0%
GAGGGTAACCAAATCCATTG+TGG + chr7_3:85144096-85144115 None:intergenic 45.0%
! GGATTTGGTTACCCTCAAGA+AGG - chr7_3:85144100-85144119 Msa1132280:intron 45.0%
!!! TTTTTTTAATGTTTTAAAAA+TGG - chr7_3:85141679-85141698 Msa1132280:CDS 5.0%
ACTCACTCACTCACTCACTC+AGG - chr7_3:85141351-85141370 Msa1132280:three_prime_UTR 50.0%
TGACAGTGAAACAACTGGGG+AGG - chr7_3:85142444-85142463 Msa1132280:CDS 50.0%
AAAGACGAAGCTCCTTCTGG+AGG - chr7_3:85142610-85142629 Msa1132280:CDS 50.0%
TGACTGGTAACTCCTCCAGA+AGG + chr7_3:85142625-85142644 None:intergenic 50.0%
ATGGTCGTGGGAGAATTGAC+TGG + chr7_3:85142641-85142660 None:intergenic 50.0%
CAAACAGCATCTCATGGTCG+TGG + chr7_3:85142654-85142673 None:intergenic 50.0%
CACGACCATGAGATGCTGTT+TGG - chr7_3:85142652-85142671 Msa1132280:CDS 50.0%
!! CAGAGTTGATACTGACCCTG+TGG - chr7_3:85142708-85142727 Msa1132280:CDS 50.0%
TCCAAGGTCTGCACCAGAAA+TGG + chr7_3:85142754-85142773 None:intergenic 50.0%
!! AAGGACTTCTTCTGGAGTGG+TGG + chr7_3:85142827-85142846 None:intergenic 50.0%
! TGCAAGGACTTCTTCTGGAG+TGG + chr7_3:85142830-85142849 None:intergenic 50.0%
! TCGCACAAGCACGCTCTAAA+CGG + chr7_3:85142957-85142976 None:intergenic 50.0%
! TTTAGAGCGTGCTTGTGCGA+TGG - chr7_3:85142957-85142976 Msa1132280:CDS 50.0%
!! TGGCTGGTCTTCATGTTCTC+AGG - chr7_3:85142977-85142996 Msa1132280:CDS 50.0%
!! GCATTGGCAGGAAGTACCAT+CGG - chr7_3:85143171-85143190 Msa1132280:CDS 50.0%
!! ATTGCTTCGAGTTGCAACCC+AGG - chr7_3:85143290-85143309 Msa1132280:intron 50.0%
TTCCACCCAAAGCAGTGTTC+AGG - chr7_3:85143329-85143348 Msa1132280:intron 50.0%
! AACCTGAACACTGCTTTGGG+TGG + chr7_3:85143334-85143353 None:intergenic 50.0%
ATGCAAGGTTGTTGACAGGG+CGG - chr7_3:85143383-85143402 Msa1132280:intron 50.0%
CACAAACCGCACCTTCCAAA+GGG + chr7_3:85143599-85143618 None:intergenic 50.0%
GTTTGTGGTGCAGCAATGGA+AGG - chr7_3:85143612-85143631 Msa1132280:intron 50.0%
! ATGGAAGGAGCTGTTGCTTC+AGG - chr7_3:85143627-85143646 Msa1132280:intron 50.0%
TCAGGCATCAGTGATCCCTT+TGG - chr7_3:85143645-85143664 Msa1132280:intron 50.0%
GCTCGAATTCCAATGGCAAG+AGG + chr7_3:85143702-85143721 None:intergenic 50.0%
GCCATTGGAATTCGAGCTGA+TGG - chr7_3:85143705-85143724 Msa1132280:intron 50.0%
CCCATCAGCTCGAATTCCAA+TGG + chr7_3:85143709-85143728 None:intergenic 50.0%
GTACGAAGGTGAGGGAAAGA+AGG - chr7_3:85143833-85143852 Msa1132280:intron 50.0%
GGAATACCTGCAGCACCAAA+AGG - chr7_3:85143894-85143913 Msa1132280:intron 50.0%
!! AACGTGCTGCGAGTTTTAGC+CGG - chr7_3:85143957-85143976 Msa1132280:intron 50.0%
AACAGGCACCACAGGTATAC+GGG + chr7_3:85144000-85144019 None:intergenic 50.0%
ACTTCCATAACAGGCACCAC+AGG + chr7_3:85144008-85144027 None:intergenic 50.0%
TGGTGCCTGTTATGGAAGTG+AGG - chr7_3:85144009-85144028 Msa1132280:intron 50.0%
GGCATCCTCACTTCCATAAC+AGG + chr7_3:85144017-85144036 None:intergenic 50.0%
GAGGCCCTAAATAGGACCTA+TGG - chr7_3:85144068-85144087 Msa1132280:intron 50.0%
!! TAGGACCTATGGTGCCACAA+TGG - chr7_3:85144079-85144098 Msa1132280:intron 50.0%
ACATCAGGTATGCACAGACG+AGG + chr7_3:85144262-85144281 None:intergenic 50.0%
TGAGTGAGTGAGTGAGCGAG+CGG + chr7_3:85141344-85141363 None:intergenic 55.0%
TGTGTGGAATGGCTCCGAAG+TGG - chr7_3:85142534-85142553 Msa1132280:CDS 55.0%
!! GCCATTTCTGGTGCAGACCT+TGG - chr7_3:85142750-85142769 Msa1132280:CDS 55.0%
CTGGTGCAGACCTTGGACAT+TGG - chr7_3:85142757-85142776 Msa1132280:CDS 55.0%
TGGACGAACGCCAATGTCCA+AGG + chr7_3:85142770-85142789 None:intergenic 55.0%
! CGCACAAGCACGCTCTAAAC+GGG + chr7_3:85142956-85142975 None:intergenic 55.0%
GATGCCTGAACCAACAGCAG+TGG - chr7_3:85143002-85143021 Msa1132280:CDS 55.0%
!! AACGCCACTGCTGTTGGTTC+AGG + chr7_3:85143009-85143028 None:intergenic 55.0%
!! TTGGCAGGAAGTACCATCGG+TGG - chr7_3:85143174-85143193 Msa1132280:CDS 55.0%
TGTTGACAGGGCGGAGTTTG+AGG - chr7_3:85143392-85143411 Msa1132280:intron 55.0%
CCACAAACCGCACCTTCCAA+AGG + chr7_3:85143600-85143619 None:intergenic 55.0%
! CCTTTGGAAGGTGCGGTTTG+TGG - chr7_3:85143597-85143616 Msa1132280:intron 55.0%
TGCGGTTTGTGGTGCAGCAA+TGG - chr7_3:85143608-85143627 Msa1132280:intron 55.0%
CAAGCCACTCCTCTTGCCAT+TGG - chr7_3:85143690-85143709 Msa1132280:intron 55.0%
GCTGGCATTGTGGTGTTCCT+TGG + chr7_3:85143753-85143772 None:intergenic 55.0%
!!! GGAACTCCTTTTGGTGCTGC+AGG + chr7_3:85143903-85143922 None:intergenic 55.0%
!! TTAGCCGGTGTTGTGATGCC+TGG - chr7_3:85143972-85143991 Msa1132280:intron 55.0%
GGATCCAGGCATCACAACAC+CGG + chr7_3:85143979-85143998 None:intergenic 55.0%
ACCACAGGTATACGGGATCC+AGG + chr7_3:85143993-85144012 None:intergenic 55.0%
! TGGTCATGGTTGGTGTGCTG+AGG - chr7_3:85144049-85144068 Msa1132280:intron 55.0%
GGTGTGCTGAGGCCCTAAAT+AGG - chr7_3:85144060-85144079 Msa1132280:intron 55.0%
TCAGGTATGCACAGACGAGG+TGG + chr7_3:85144259-85144278 None:intergenic 55.0%
! CACGCTCTAAACGGGTCAGC+TGG + chr7_3:85142948-85142967 None:intergenic 60.0%
GTTGCTGTGACTGCTACCGC+CGG - chr7_3:85143129-85143148 Msa1132280:CDS 60.0%
TTGCTGTGACTGCTACCGCC+GGG - chr7_3:85143130-85143149 Msa1132280:CDS 60.0%
! TATCTGGGATACTCCCCCGG+CGG + chr7_3:85143148-85143167 None:intergenic 60.0%
TGACAGGGCGGAGTTTGAGG+AGG - chr7_3:85143395-85143414 Msa1132280:intron 60.0%
GCCTGGATCCCGTATACCTG+TGG - chr7_3:85143989-85144008 Msa1132280:intron 60.0%
GAGCGTGCTTGTGCGATGGC+TGG - chr7_3:85142961-85142980 Msa1132280:CDS 65.0%
TGCTGTGACTGCTACCGCCG+GGG - chr7_3:85143131-85143150 Msa1132280:CDS 65.0%
GCTGTGACTGCTACCGCCGG+GGG - chr7_3:85143132-85143151 Msa1132280:CDS 70.0%
Chromosome Type Strat End Strand Name
chr7_3 gene 85141274 85144335 85141274 ID=Msa1132280;Name=Msa1132280
chr7_3 mRNA 85141274 85144335 85141274 ID=Msa1132280-mRNA-1;Parent=Msa1132280;Name=Msa1132280-mRNA-1;_AED=0.00;_eAED=0.00;_QI=99|0|0.5|1|0|0.5|2|208|572
chr7_3 exon 85141274 85143200 85141274 ID=Msa1132280-mRNA-1:exon:25379;Parent=Msa1132280-mRNA-1
chr7_3 exon 85144237 85144335 85144237 ID=Msa1132280-mRNA-1:exon:25378;Parent=Msa1132280-mRNA-1
chr7_3 five_prime_UTR 85144237 85144335 85144237 ID=Msa1132280-mRNA-1:five_prime_utr;Parent=Msa1132280-mRNA-1
chr7_3 CDS 85141482 85143200 85141482 ID=Msa1132280-mRNA-1:cds;Parent=Msa1132280-mRNA-1
chr7_3 three_prime_UTR 85141274 85141481 85141274 ID=Msa1132280-mRNA-1:three_prime_utr;Parent=Msa1132280-mRNA-1
Gene Sequence

>Msa1132280

ATGGCAGAACGAGAACCTGCATATACCGTTGCATCTGACAGTGAAACAACTGGGGAGGAAAAATCATCACCTCTTCCTGAAATAGCGATTGGAATTGATATTGGCACCTCACAATGTAGTGTTGCTGTGTGGAATGGCTCCGAAGTGGAGCTTTTGAAAAACAAAAGAAATCAGAAGCTTATGAAGTCATTTGTGACTTTCAAAGACGAAGCTCCTTCTGGAGGAGTTACCAGTCAATTCTCCCACGACCATGAGATGCTGTTTGGAGATACAATTTTCAATATGAAACGCTTGATCGGCAGAGTTGATACTGACCCTGTGGTTCATGCAAGTAAAAATCTGCCATTTCTGGTGCAGACCTTGGACATTGGCGTTCGTCCATTTATTGCTGCGTTAGTGAACAATGTCTGGAGATCCACCACTCCAGAAGAAGTCCTTGCAATGTTTCTGGTGGAGTTAAGACTGATGACTGAAACTCATCTCAAACGACCGATAAGAAATGTGGTGCTTACTGTTCCGGTTTCGTTCAGTCGTTTCCAGCTGACCCGTTTAGAGCGTGCTTGTGCGATGGCTGGTCTTCATGTTCTCAGGTTGATGCCTGAACCAACAGCAGTGGCGTTATTATATGGACAACAACAACAGAAAGCTTCTCAAGAAACTATGGGCAGTGGAAGTGAGAAAATTGCTCTCATTTTCAATATGGGTGCTGGTTATTGTGATGTTGCTGTGACTGCTACCGCCGGGGGAGTATCCCAGATAAAAGCATTGGCAGGAAGTACCATCGGTGGAGAAGATTTGGTTCAGAATATGATGTGTTATCTCTTACCAGATTCTGAAAATATATTCAAGCGTCATGGGGTCAAAGAAATTAAATCAATGGCATTGCTTCGAGTTGCAACCCAGGATGCAATTCATCAGCTTTCCACCCAAAGCAGTGTTCAGGTTGATGTAGATTTGGGAGATGGTTTGAAGATATGCAAGGTTGTTGACAGGGCGGAGTTTGAGGAGGTGAACAAAGAGGTGTTCGAAAAGTGTGAAAGCCTTATCATCCAATGTTTACATGATGCAAAGGTAGAAGTTGGGGATATAAATGATGTGATACTTGTTGGAGGATGTTCTTATATCCCAAGGGTGGAGAATCTTGTTACAACCTTATGCATAATAACAGAAGTTTATAAAGGTATAAACCCTTTGGAAGGTGCGGTTTGTGGTGCAGCAATGGAAGGAGCTGTTGCTTCAGGCATCAGTGATCCCTTTGGAAACTTGGACTTGTTAACGATTCAAGCCACTCCTCTTGCCATTGGAATTCGAGCTGATGGGAACAAGTTTGTACCTGTAATTCCAAGGAACACCACAATGCCAGCACGTAAAGATCTACTTTTCACAACTATTCATGATAATCAAACTGAGGCATTGATACTTGTGTACGAAGGTGAGGGAAAGAAGGCAGAAGAAAACCACTTGTTAGGATATTTCAAGATAATGGGAATACCTGCAGCACCAAAAGGAGTTCCTGAAATCAGTGTGTGCATGGACATAGATGCTGCAAACGTGCTGCGAGTTTTAGCCGGTGTTGTGATGCCTGGATCCCGTATACCTGTGGTGCCTGTTATGGAAGTGAGGATGCCAATGGTTGATGATGGTCATGGTTGGTGTGCTGAGGCCCTAAATAGGACCTATGGTGCCACAATGGATTTGGTTACCCTCAAGAAGGCATAA
Protein sequence

>Msa1132280

MAEREPAYTVASDSETTGEEKSSPLPEIAIGIDIGTSQCSVAVWNGSEVELLKNKRNQKLMKSFVTFKDEAPSGGVTSQFSHDHEMLFGDTIFNMKRLIGRVDTDPVVHASKNLPFLVQTLDIGVRPFIAALVNNVWRSTTPEEVLAMFLVELRLMTETHLKRPIRNVVLTVPVSFSRFQLTRLERACAMAGLHVLRLMPEPTAVALLYGQQQQKASQETMGSGSEKIALIFNMGAGYCDVAVTATAGGVSQIKALAGSTIGGEDLVQNMMCYLLPDSENIFKRHGVKEIKSMALLRVATQDAIHQLSTQSSVQVDVDLGDGLKICKVVDRAEFEEVNKEVFEKCESLIIQCLHDAKVEVGDINDVILVGGCSYIPRVENLVTTLCIITEVYKGINPLEGAVCGAAMEGAVASGISDPFGNLDLLTIQATPLAIGIRADGNKFVPVIPRNTTMPARKDLLFTTIHDNQTEALILVYEGEGKKAEENHLLGYFKIMGIPAAPKGVPEISVCMDIDAANVLRVLAGVVMPGSRIPVVPVMEVRMPMVDDGHGWCAEALNRTYGATMDLVTLKKA*