AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1419590

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1419590 MtrunA17_Chr5g0394391 98.077 572 11 0 1 572 1 572 0.0 1155
Msa1419590 MtrunA17_Chr4g0064621 61.513 595 200 8 1 571 101 690 0.0 668
Msa1419590 MtrunA17_Chr4g0060241 57.041 561 206 13 19 569 10 545 0.0 575
Msa1419590 MtrunA17_Chr5g0442171 85.152 330 41 2 190 518 1 323 0.0 568
Msa1419590 MtrunA17_Chr7g0223181 37.228 505 290 10 29 523 9 496 3.73e-99 314
Msa1419590 MtrunA17_Chr5g0442161 83.721 172 27 1 8 178 7 178 4.52e-99 298
Msa1419590 MtrunA17_Chr7g0223291 37.426 505 289 10 29 523 9 496 1.19e-98 313
Msa1419590 MtrunA17_Chr4g0073741 36.853 502 296 10 29 523 8 495 1.36e-98 313
Msa1419590 MtrunA17_Chr4g0031321 36.634 505 293 10 29 523 9 496 4.73e-97 309
Msa1419590 MtrunA17_Chr7g0262821 36.170 517 279 11 29 523 9 496 7.78e-97 308
Msa1419590 MtrunA17_Chr4g0031301 36.832 505 292 10 29 523 9 496 1.04e-96 308
Msa1419590 MtrunA17_Chr2g0298851 35.509 521 292 14 29 530 9 504 5.32e-88 284
Msa1419590 MtrunA17_Chr2g0296391 35.798 514 300 12 29 530 9 504 1.46e-87 281
Msa1419590 MtrunA17_Chr8g0350731 34.328 536 320 14 8 531 16 531 4.38e-87 283
Msa1419590 MtrunA17_Chr7g0223721 34.308 513 308 12 29 530 9 503 6.56e-87 281
Msa1419590 MtrunA17_Chr7g0233311 36.275 510 305 10 29 531 9 505 2.33e-86 280
Msa1419590 MtrunA17_Chr2g0293581 35.897 507 295 13 29 523 10 498 8.27e-86 278
Msa1419590 MtrunA17_Chr7g0223851 36.078 510 303 13 29 530 18 512 8.64e-86 279
Msa1419590 MtrunA17_Chr7g0223621 33.704 540 328 13 29 557 9 529 2.94e-85 277
Msa1419590 MtrunA17_Chr7g0223781 33.640 544 323 14 29 554 9 532 1.05e-84 275
Msa1419590 MtrunA17_Chr7g0223801 34.291 522 316 13 28 539 8 512 8.22e-84 273
Msa1419590 MtrunA17_Chr8g0388761 33.779 524 298 12 19 523 29 522 1.09e-83 274
Msa1419590 MtrunA17_Chr2g0284681 33.138 513 306 11 29 523 8 501 1.47e-83 272
Msa1419590 MtrunA17_Chr8g0388671 33.015 524 308 10 16 523 26 522 3.24e-83 273
Msa1419590 MtrunA17_Chr7g0223711 34.653 505 305 11 28 523 8 496 6.74e-83 271
Msa1419590 MtrunA17_Chr7g0225671 35.534 515 298 17 29 530 16 509 1.49e-82 270
Msa1419590 MtrunA17_Chr7g0221661 35.714 504 299 11 29 523 9 496 1.50e-81 267
Msa1419590 MtrunA17_Chr5g0427901 35.135 518 294 15 29 523 9 507 1.40e-80 265
Msa1419590 MtrunA17_Chr7g0223741 33.789 512 314 11 29 531 9 504 2.22e-80 264
Msa1419590 MtrunA17_Chr2g0287631 36.505 515 297 12 28 530 8 504 9.20e-80 263
Msa1419590 MtrunA17_Chr7g0223821 35.465 516 297 17 29 529 9 503 9.89e-80 265
Msa1419590 MtrunA17_Chr7g0223751 33.270 523 317 14 29 539 9 511 2.80e-79 261
Msa1419590 MtrunA17_Chr2g0298861 33.141 519 318 14 30 531 10 516 3.61e-79 261
Msa1419590 MtrunA17_Chr7g0223731 33.268 514 313 15 29 531 9 503 5.67e-78 258
Msa1419590 MtrunA17_Chr7g0233301 34.638 511 281 13 29 531 9 474 2.48e-76 253
Msa1419590 MtrunA17_Chr4g0040931 33.399 506 308 13 29 523 9 496 3.08e-76 253
Msa1419590 MtrunA17_Chr7g0223631 34.357 521 302 14 28 539 8 497 5.50e-76 252
Msa1419590 MtrunA17_Chr6g0462681 31.898 511 308 11 27 521 7 493 7.60e-76 253
Msa1419590 MtrunA17_Chr6g0462601 32.495 517 301 14 27 523 7 495 3.46e-75 251
Msa1419590 MtrunA17_Chr7g0248151 35.078 516 302 17 29 531 9 504 2.92e-74 248
Msa1419590 MtrunA17_Chr7g0223831 34.698 513 278 16 29 530 9 475 3.40e-74 248
Msa1419590 MtrunA17_Chr7g0223031 33.074 514 314 14 29 531 9 503 4.74e-74 247
Msa1419590 MtrunA17_Chr7g0223541 36.986 438 249 11 96 523 33 453 1.14e-72 243
Msa1419590 MtrunA17_Chr6g0462771 32.277 505 319 12 27 521 7 498 3.71e-72 243
Msa1419590 MtrunA17_Chr4g0012541 32.617 512 309 13 29 530 9 494 1.08e-71 241
Msa1419590 MtrunA17_Chr7g0248131 33.977 518 307 17 29 531 9 506 3.49e-71 240
Msa1419590 MtrunA17_Chr6g0462641 33.268 508 310 14 27 521 7 498 5.31e-71 241
Msa1419590 MtrunA17_Chr2g0279901 31.141 517 293 11 30 523 57 533 4.60e-70 238
Msa1419590 MtrunA17_Chr7g0223701 32.233 515 312 12 28 523 8 504 1.21e-69 236
Msa1419590 MtrunA17_Chr4g0049501 29.433 547 314 12 5 523 29 531 3.37e-69 236
Msa1419590 MtrunA17_Chr6g0462711 32.759 522 295 16 27 521 8 500 4.71e-69 236
Msa1419590 MtrunA17_Chr7g0217971 32.745 510 295 13 29 530 9 478 1.04e-68 233
Msa1419590 MtrunA17_Chr7g0215261 33.592 515 275 18 29 530 9 469 1.80e-68 232
Msa1419590 MtrunA17_Chr7g0223681 34.987 383 237 6 143 523 34 406 9.29e-67 226
Msa1419590 MtrunA17_Chr2g0293601 31.881 505 282 14 28 521 8 461 4.59e-64 218
Msa1419590 MtrunA17_Chr6g0462611 32.401 429 276 8 96 521 24 441 5.65e-64 221
Msa1419590 MtrunA17_Chr2g0277021 32.157 510 305 16 27 523 69 550 8.77e-62 216
Msa1419590 MtrunA17_Chr8g0390821 30.104 578 285 19 27 530 9 541 2.77e-60 211
Msa1419590 MtrunA17_Chr7g0228281 31.128 514 278 16 29 530 70 519 3.78e-57 202
Msa1419590 MtrunA17_Chr2g0293561 33.952 377 231 9 28 397 8 373 1.20e-55 192
Msa1419590 MtrunA17_Chr2g0299821 30.063 479 290 15 30 486 10 465 6.12e-55 193
Msa1419590 MtrunA17_Chr2g0331381 33.333 441 236 16 30 457 7 402 2.32e-53 189
Msa1419590 MtrunA17_Chr2g0299791 33.753 397 233 12 29 414 1042 1419 8.50e-53 194
Msa1419590 MtrunA17_Chr6g0462861 29.600 500 310 14 29 521 9 473 1.16e-52 190
Msa1419590 MtrunA17_Chr2g0299921 32.836 402 224 10 29 411 1029 1403 9.26e-52 191
Msa1419590 MtrunA17_Chr2g0299921 33.248 391 214 11 29 399 1413 1776 1.12e-50 188
Msa1419590 MtrunA17_Chr2g0299851 31.633 392 246 9 29 413 1038 1414 1.24e-47 179
Msa1419590 MtrunA17_Chr2g0299881 32.242 397 241 11 30 414 1039 1419 6.19e-47 177
Msa1419590 MtrunA17_Chr4g0064651 67.480 123 40 0 395 517 1 123 2.42e-45 156
Msa1419590 MtrunA17_Chr7g0245531 39.713 209 121 3 328 535 1 205 1.19e-40 149
Msa1419590 MtrunA17_Chr2g0293271 29.399 449 229 12 41 483 43 409 3.66e-40 150
Msa1419590 MtrunA17_Chr4g0057741 61.947 113 41 1 234 344 1 113 1.51e-38 137
Msa1419590 MtrunA17_Chr1g0151991 34.256 289 167 9 29 308 9 283 1.53e-38 143
Msa1419590 MtrunA17_Chr1g0166551 25.301 498 243 12 29 521 5 378 1.54e-34 137
Msa1419590 MtrunA17_Chr7g0245521 32.143 280 169 8 28 300 8 273 1.58e-34 131
Msa1419590 MtrunA17_Chr1g0152001 38.542 192 111 5 340 530 2 187 9.71e-34 130
Msa1419590 MtrunA17_Chr4g0057751 53.846 104 48 0 118 221 1 104 5.14e-29 110
Msa1419590 MtrunA17_Chr1g0188611 36.216 185 113 3 348 531 3 183 7.74e-28 113
Msa1419590 MtrunA17_Chr4g0004331 25.316 474 315 11 30 476 4 465 1.78e-27 117
Msa1419590 MtrunA17_Chr3g0098761 25.799 438 291 13 30 455 4 419 5.29e-26 113
Msa1419590 MtrunA17_Chr7g0223761 33.161 193 117 6 270 456 7 193 1.57e-25 108
Msa1419590 MtrunA17_Chr5g0394221 32.995 197 124 3 329 523 75 265 4.56e-25 107
Msa1419590 MtrunA17_Chr4g0064661 57.143 84 32 2 95 177 1 81 1.14e-23 95.1
Msa1419590 MtrunA17_Chr2g0331901 23.608 449 312 10 30 461 4 438 2.84e-23 104
Msa1419590 MtrunA17_Chr6g0462751 25.348 359 196 12 82 436 11 301 3.87e-22 97.4
Msa1419590 MtrunA17_Chr5g0444001 24.719 445 301 13 30 459 4 429 3.95e-22 100
Msa1419590 MtrunA17_Chr7g0223771 33.557 149 91 3 126 273 14 155 1.91e-21 91.7
Msa1419590 MtrunA17_Chr5g0442181 85.106 47 7 0 526 572 1 47 2.08e-21 87.4
Msa1419590 MtrunA17_Chr7g0223671 31.633 196 121 5 86 279 4 188 2.84e-21 92.0
Msa1419590 MtrunA17_Chr6g0462631 23.739 337 204 8 108 440 8 295 3.08e-21 94.4
Msa1419590 MtrunA17_Chr6g0462691 35.461 141 90 1 348 488 3 142 5.18e-19 84.3
Msa1419590 MtrunA17_Chr3g0120101 22.846 499 313 15 23 479 20 488 2.69e-18 89.0
Msa1419590 MtrunA17_Chr1g0188601 30.481 187 115 4 126 307 15 191 2.80e-18 83.6
Msa1419590 MtrunA17_Chr7g0245541 46.667 90 47 1 446 535 4 92 1.66e-16 78.6
Msa1419590 MtrunA17_Chr4g0046881 35.075 134 76 4 158 290 17 140 5.90e-16 75.5
Msa1419590 MtrunA17_Chr6g0462791 32.168 143 89 2 134 275 7 142 3.44e-15 73.9
Msa1419590 MtrunA17_Chr7g0223861 36.364 121 75 2 95 214 1 120 5.65e-15 72.0
Msa1419590 MtrunA17_Chr4g0046871 39.000 100 57 2 363 461 9 105 2.17e-13 66.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1419590 AT2G32120.1 73.860 570 135 2 5 571 3 561 0.0 897
Msa1419590 AT2G32120.2 73.860 570 135 2 5 571 3 561 0.0 897
Msa1419590 AT3G12580.1 37.426 505 289 10 29 523 9 496 2.18e-102 323
Msa1419590 AT5G02500.1 37.030 505 291 10 29 523 9 496 1.34e-99 316
Msa1419590 AT3G09440.1 37.228 505 290 10 29 523 9 496 2.52e-98 313
Msa1419590 AT3G09440.4 37.228 505 290 10 29 523 9 496 2.52e-98 313
Msa1419590 AT3G09440.2 37.228 505 290 10 29 523 9 496 2.52e-98 313
Msa1419590 AT3G09440.3 37.228 505 290 10 29 523 9 496 2.52e-98 313
Msa1419590 AT1G16030.1 36.047 516 285 10 27 523 6 495 5.17e-98 311
Msa1419590 AT1G56410.1 37.030 505 291 10 29 523 9 496 6.39e-95 303
Msa1419590 AT5G02490.1 35.842 505 297 10 29 523 9 496 2.46e-94 302
Msa1419590 AT1G09080.2 34.469 499 306 8 30 523 43 525 6.67e-86 280
Msa1419590 AT1G09080.1 34.469 499 306 8 30 523 53 535 7.51e-86 280
Msa1419590 AT5G42020.1 33.074 514 321 10 16 523 25 521 2.14e-84 276
Msa1419590 AT5G42020.3 33.074 514 321 10 16 523 30 526 4.16e-84 276
Msa1419590 AT5G28540.1 33.600 500 310 9 30 523 38 521 3.99e-83 273
Msa1419590 AT5G42020.2 33.268 511 317 11 16 519 25 518 4.98e-83 271
Msa1419590 AT5G09590.1 30.651 522 306 10 22 523 52 537 2.54e-67 231
Msa1419590 AT4G37910.1 30.857 525 301 11 22 523 47 532 3.17e-64 223
Msa1419590 AT4G37910.2 30.857 525 301 11 22 523 47 532 3.17e-64 223
Msa1419590 AT5G02500.2 34.097 393 239 8 29 414 9 388 3.82e-63 216
Msa1419590 AT5G49910.1 31.299 508 312 14 27 523 78 559 1.41e-60 214
Msa1419590 AT4G24280.1 31.434 509 310 15 27 523 78 559 2.48e-59 210
Msa1419590 AT1G11660.4 26.464 461 305 16 30 476 4 444 2.22e-27 117
Msa1419590 AT1G11660.3 26.464 461 305 16 30 476 4 444 2.22e-27 117
Msa1419590 AT1G11660.1 26.464 461 305 16 30 476 4 444 2.22e-27 117
Msa1419590 AT1G79930.1 24.944 449 306 10 30 461 4 438 5.46e-26 113
Msa1419590 AT1G79930.2 24.944 449 306 10 30 461 4 438 6.14e-26 113
Msa1419590 AT1G11660.2 27.250 400 259 15 91 476 26 407 5.85e-24 107
Msa1419590 AT1G79920.1 24.499 449 308 10 30 461 4 438 8.79e-24 106
Msa1419590 AT1G79920.3 24.499 449 308 10 30 461 4 438 9.52e-24 106
Msa1419590 AT1G79920.2 24.499 449 308 10 30 461 4 438 9.52e-24 106
Msa1419590 AT1G79920.4 24.499 449 308 10 30 461 4 438 9.52e-24 106
Msa1419590 AT4G16660.2 25.000 360 237 10 143 480 164 512 4.39e-19 92.0
Msa1419590 AT4G16660.1 25.000 360 237 10 143 480 140 488 4.78e-19 91.7

Find 168 sgRNAs with CRISPR-Local

Find 232 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTAGGATATTTCAAGATAA+TGG 0.182846 tig0024721:+7172 Msa1419590:CDS
TGGCACCATAGGTCCTATTT+AGG 0.200225 tig0024721:-7373 None:intergenic
AGAAGTCCTTGCAATGTTTC+TGG 0.201625 tig0024721:+6137 Msa1419590:CDS
TCCATGCACACACTGATTTC+AGG 0.246998 tig0024721:-7221 None:intergenic
TTCACTAACGCAGCAATAAA+TGG 0.261599 tig0024721:-6087 None:intergenic
CAGTGATCCCTTTGGAAACT+TGG 0.262026 tig0024721:+6953 Msa1419590:CDS
TCAATTCCAATCGCTATTTC+AGG 0.266817 tig0024721:-5784 None:intergenic
GTGGTGTTCCTTGGAATTAC+AGG 0.282170 tig0024721:-7041 None:intergenic
CTGATTTCAGGAACTCCTTT+TGG 0.283375 tig0024721:-7209 None:intergenic
TTCAAAGACGAAGCTCCTTC+TGG 0.283594 tig0024721:+5907 Msa1419590:CDS
GAAAGCTTCTCAAGAAACTA+TGG 0.285521 tig0024721:+6350 Msa1419590:CDS
TGTTCAGGTTGATGTAGATT+TGG 0.316072 tig0024721:+6644 Msa1419590:CDS
CTATGGTGCCACAATGGATT+TGG 0.318656 tig0024721:+7385 Msa1419590:CDS
AACATTGCAAGGACTTCTTC+TGG 0.325894 tig0024721:-6132 None:intergenic
AAATGTGGTGCTTACTGTTC+CGG 0.327314 tig0024721:+6206 Msa1419590:CDS
TTAGGATATTTCAAGATAAT+GGG 0.332403 tig0024721:+7173 Msa1419590:CDS
ACAGCAGTGGCGTTATTATA+TGG 0.334823 tig0024721:+6315 Msa1419590:CDS
ATAGCGATTGGAATTGATAT+TGG 0.340874 tig0024721:+5790 Msa1419590:CDS
TAACAGGCACCACAGGTATA+CGG 0.347738 tig0024721:-7298 None:intergenic
TTCTAATTAATACAAACAAC+AGG 0.351814 tig0024721:-4497 Msa1419580:intron
GGCACCATAGGTCCTATTTA+GGG 0.356331 tig0024721:-7372 None:intergenic
TACCATCGGTGGAGAAGATT+TGG 0.359082 tig0024721:+6485 Msa1419590:CDS
GTTCAGGTTGATGTAGATTT+GGG 0.369664 tig0024721:+6645 Msa1419590:CDS
GGCATCCTCACTTCCATAAC+AGG 0.369992 tig0024721:-7314 None:intergenic
AACGCCACTGCTGTTGGTTC+AGG 0.370548 tig0024721:-6306 None:intergenic
ATCAACCTGAACACTGCTTT+GGG 0.377089 tig0024721:-6634 None:intergenic
ATGGAAGGAGCTGTTGCTTC+AGG 0.378268 tig0024721:+6927 Msa1419590:CDS
CATCAACCTGAACACTGCTT+TGG 0.383070 tig0024721:-6635 None:intergenic
TTCCACCCAAAGCAGTGTTC+AGG 0.388302 tig0024721:+6629 Msa1419590:CDS
GTAAGCACCACATTTCTTAT+CGG 0.392583 tig0024721:-6198 None:intergenic
CACGACCATGAGATGCTGTT+TGG 0.393050 tig0024721:+5952 Msa1419590:CDS
TCGCACAAGCACGCTCTAAA+CGG 0.395103 tig0024721:-6254 None:intergenic
TATACCTGTGGTGCCTGTTA+TGG 0.395756 tig0024721:+7301 Msa1419590:CDS
CATGTAAACATTGGATGATA+AGG 0.397163 tig0024721:-6749 None:intergenic
TCCAAGGTCTGCACCAGAAA+TGG 0.397431 tig0024721:-6051 None:intergenic
GCAGAAGAAAACCACTTGTT+AGG 0.403341 tig0024721:+7155 Msa1419590:CDS
TGGCTGGTCTTCATGTTCTC+AGG 0.414478 tig0024721:+6277 Msa1419590:CDS
TTATAAAGGTATAAACCCTT+TGG 0.418915 tig0024721:+6881 Msa1419590:CDS
AAAATAACAGAAGTTTATAA+AGG 0.432441 tig0024721:+6867 Msa1419590:CDS
CATGATGCAAAGGTAGAAGT+TGG 0.434870 tig0024721:+6768 Msa1419590:CDS
CCTTTGGAAGGTGCGGTTTG+TGG 0.435575 tig0024721:+6897 Msa1419590:CDS
CACGCTCTAAACGGGTCAGC+TGG 0.440361 tig0024721:-6245 None:intergenic
GGTGTGCTGAGGCCCTAAAT+AGG 0.444919 tig0024721:+7360 Msa1419590:CDS
GCCATTTCTGGTGCAGACCT+TGG 0.447888 tig0024721:+6050 Msa1419590:CDS
ATGATGCAAAGGTAGAAGTT+GGG 0.448859 tig0024721:+6769 Msa1419590:CDS
GCTGGCATTGTGGTGTTCCT+TGG 0.449119 tig0024721:-7050 None:intergenic
GCATCTGACAGTGAAACAAC+TGG 0.449771 tig0024721:+5739 Msa1419590:CDS
ATCAATTTATGCCTTCTTGA+GGG 0.450343 tig0024721:-7411 None:intergenic
CTGCGTTAGTGAACAATGTC+TGG 0.455549 tig0024721:+6097 Msa1419590:CDS
TCAGGCATCAGTGATCCCTT+TGG 0.456587 tig0024721:+6945 Msa1419590:CDS
TGTTGACAGGGCGGAGTTTG+AGG 0.457797 tig0024721:+6692 Msa1419590:CDS
CTGGTGCAGACCTTGGACAT+TGG 0.457903 tig0024721:+6057 Msa1419590:CDS
GCCATTGGAATTCGAGCTGA+TGG 0.458770 tig0024721:+7005 Msa1419590:CDS
AAAATATATTCAAGCGTCAT+GGG 0.460640 tig0024721:+6544 Msa1419590:CDS
ATAAATGATGTGATACTTGT+TGG 0.460986 tig0024721:+6795 Msa1419590:CDS
CGCACAAGCACGCTCTAAAC+GGG 0.463935 tig0024721:-6253 None:intergenic
GTAACAAGATTCTCCACCCT+TGG 0.466538 tig0024721:-6834 None:intergenic
GAGCGTGCTTGTGCGATGGC+TGG 0.467592 tig0024721:+6261 Msa1419590:CDS
AATATGAGAGAGAAAACAAA+CGG 0.470264 tig0024721:-4594 Msa1419580:intron
TTAGCCGGTGTTGTGATGCC+TGG 0.471501 tig0024721:+7272 Msa1419590:CDS
TGGAAACGACTGAATGAAAC+CGG 0.482736 tig0024721:-6225 None:intergenic
CAAGCCACTCCTCTTGCCAT+TGG 0.482744 tig0024721:+6990 Msa1419590:CDS
CCAATCGCTATTTCAGGAAG+AGG 0.484412 tig0024721:-5778 None:intergenic
GTTGATGTAGATTTGGGAGA+TGG 0.485617 tig0024721:+6651 Msa1419590:CDS
TATAATAACGCCACTGCTGT+TGG 0.489694 tig0024721:-6312 None:intergenic
GGTCAAAGAAATTAAATCAA+TGG 0.491607 tig0024721:+6566 Msa1419590:CDS
TAACAAGATTCTCCACCCTT+GGG 0.492275 tig0024721:-6833 None:intergenic
GAAAATATATTCAAGCGTCA+TGG 0.496398 tig0024721:+6543 Msa1419590:CDS
CAAATCCATTGTGGCACCAT+AGG 0.497810 tig0024721:-7384 None:intergenic
AAAGCTGATGAATTGCATCC+TGG 0.503417 tig0024721:-6608 None:intergenic
CCTCTTCCTGAAATAGCGAT+TGG 0.506297 tig0024721:+5778 Msa1419590:CDS
GTTGCTGTGACTGCTACCGC+CGG 0.506470 tig0024721:+6429 Msa1419590:CDS
TTGCTGTGACTGCTACCGCC+GGG 0.511136 tig0024721:+6430 Msa1419590:CDS
AACAAGTCCAAGTTTCCAAA+GGG 0.511681 tig0024721:-6960 None:intergenic
AGATATGCAAGGTTGTTGAC+AGG 0.512232 tig0024721:+6679 Msa1419590:CDS
TATCAATTTATGCCTTCTTG+AGG 0.516603 tig0024721:-7412 None:intergenic
AACAGGCACCACAGGTATAC+GGG 0.517047 tig0024721:-7297 None:intergenic
GGAATACCTGCAGCACCAAA+AGG 0.524506 tig0024721:+7194 Msa1419590:CDS
CAAACAGCATCTCATGGTCG+TGG 0.528773 tig0024721:-5951 None:intergenic
CACAAACCGCACCTTCCAAA+GGG 0.529952 tig0024721:-6896 None:intergenic
TTCAATATGAAACGCTTGAT+CGG 0.530493 tig0024721:+5985 Msa1419590:CDS
CCTTTGCATCATGTAAACAT+TGG 0.531721 tig0024721:-6758 None:intergenic
GAGGCCCTAAATAGGACCTA+TGG 0.533257 tig0024721:+7368 Msa1419590:CDS
TGCGGTTTGTGGTGCAGCAA+TGG 0.533347 tig0024721:+6908 Msa1419590:CDS
ACCACAGGTATACGGGATCC+AGG 0.535468 tig0024721:-7290 None:intergenic
TCCTGAAATCAGTGTGTGCA+TGG 0.540172 tig0024721:+7220 Msa1419590:CDS
TCTTGAAATATCCTAACAAG+TGG 0.545629 tig0024721:-7166 None:intergenic
AAAAGTAGATCTTTACGTGC+TGG 0.548488 tig0024721:-7068 None:intergenic
AACCTGAACACTGCTTTGGG+TGG 0.551609 tig0024721:-6631 None:intergenic
AAGGACTTCTTCTGGAGTGG+TGG 0.559811 tig0024721:-6124 None:intergenic
TGCAAGGACTTCTTCTGGAG+TGG 0.559852 tig0024721:-6127 None:intergenic
TCCCAGATAAAAGCATTGGC+AGG 0.560848 tig0024721:+6459 Msa1419590:CDS
TCTCAAGAAACTATGGGCAG+TGG 0.563843 tig0024721:+6357 Msa1419590:CDS
GGATTTGGTTACCCTCAAGA+AGG 0.565408 tig0024721:+7400 Msa1419590:CDS
ATATGAGAGAGAAAACAAAC+GGG 0.566282 tig0024721:-4593 Msa1419580:intron
AACCAAATCTTCTCCACCGA+TGG 0.568389 tig0024721:-6487 None:intergenic
TGGACGAACGCCAATGTCCA+AGG 0.570950 tig0024721:-6067 None:intergenic
GTACGAAGGTGAGGGAAAGA+AGG 0.571023 tig0024721:+7133 Msa1419590:CDS
TTTAGAGCGTGCTTGTGCGA+TGG 0.572607 tig0024721:+6257 Msa1419590:CDS
TCAGATGCAACGGTATATGC+AGG 0.578112 tig0024721:-5724 None:intergenic
GATACTTGTGTACGAAGGTG+AGG 0.578994 tig0024721:+7124 Msa1419590:CDS
AAAGGTATAAACCCTTTGGA+AGG 0.581870 tig0024721:+6885 Msa1419590:CDS
TGTTTCACTGTCAGATGCAA+CGG 0.582470 tig0024721:-5734 None:intergenic
CCCATCAGCTCGAATTCCAA+TGG 0.583989 tig0024721:-7006 None:intergenic
TAACAAGTCCAAGTTTCCAA+AGG 0.584586 tig0024721:-6961 None:intergenic
GAGGATGTTCTTATATCCCA+AGG 0.584781 tig0024721:+6817 Msa1419590:CDS
CCATGACCATCATCAACCAT+TGG 0.585720 tig0024721:-7335 None:intergenic
AGTCCTTGCAATGTTTCTGG+TGG 0.586924 tig0024721:+6140 Msa1419590:CDS
AAGCTGATGAATTGCATCCT+GGG 0.587754 tig0024721:-6607 None:intergenic
AGTATCCCAGATAAAAGCAT+TGG 0.589832 tig0024721:+6455 Msa1419590:CDS
ATTGCTTCGAGTTGCAACCC+AGG 0.590200 tig0024721:+6590 Msa1419590:CDS
CCACAAACCGCACCTTCCAA+AGG 0.591096 tig0024721:-6897 None:intergenic
TGTAGTGTTGCTGTGTGGAA+TGG 0.591608 tig0024721:+5823 Msa1419590:CDS
TGATGCAAAGGTAGAAGTTG+GGG 0.591780 tig0024721:+6770 Msa1419590:CDS
ACTTCCATAACAGGCACCAC+AGG 0.592348 tig0024721:-7305 None:intergenic
CCAATGTTTACATGATGCAA+AGG 0.592560 tig0024721:+6758 Msa1419590:CDS
TGCTGTGACTGCTACCGCCG+GGG 0.595836 tig0024721:+6431 Msa1419590:CDS
CACAGCAACACTACATTGTG+AGG 0.598044 tig0024721:-5815 None:intergenic
CATCTGACAGTGAAACAACT+GGG 0.598563 tig0024721:+5740 Msa1419590:CDS
ATGGTCGTGGGAGAATTGAC+TGG 0.601519 tig0024721:-5938 None:intergenic
CCATTGGAATTCGAGCTGAT+GGG 0.601553 tig0024721:+7006 Msa1419590:CDS
AGTTTGTACCTGTAATTCCA+AGG 0.609145 tig0024721:+7033 Msa1419590:CDS
AAAGACGAAGCTCCTTCTGG+AGG 0.611357 tig0024721:+5910 Msa1419590:CDS
ATCTTTACGTGCTGGCATTG+TGG 0.613475 tig0024721:-7060 None:intergenic
AATTCCAATGGCAAGAGGAG+TGG 0.613750 tig0024721:-6994 None:intergenic
AAACAGCATCTCATGGTCGT+GGG 0.616728 tig0024721:-5950 None:intergenic
TATCTCCAAACAGCATCTCA+TGG 0.617418 tig0024721:-5957 None:intergenic
GTTTGTGGTGCAGCAATGGA+AGG 0.619096 tig0024721:+6912 Msa1419590:CDS
TGGTCATGGTTGGTGTGCTG+AGG 0.620833 tig0024721:+7349 Msa1419590:CDS
AAAGCTTCTCAAGAAACTAT+GGG 0.624225 tig0024721:+6351 Msa1419590:CDS
AATGATGTGATACTTGTTGG+AGG 0.629718 tig0024721:+6798 Msa1419590:CDS
TAGGACCTATGGTGCCACAA+TGG 0.630018 tig0024721:+7379 Msa1419590:CDS
TGGTGCCTGTTATGGAAGTG+AGG 0.631903 tig0024721:+7309 Msa1419590:CDS
TGACAGGGCGGAGTTTGAGG+AGG 0.633647 tig0024721:+6695 Msa1419590:CDS
TATAAACCCTTTGGAAGGTG+CGG 0.634750 tig0024721:+6890 Msa1419590:CDS
GCATTGGCAGGAAGTACCAT+CGG 0.637875 tig0024721:+6471 Msa1419590:CDS
AGGATGCCAATGGTTGATGA+TGG 0.639984 tig0024721:+7329 Msa1419590:CDS
GCTCACTCACTCACTCACTC+AGG 0.644678 tig0024721:+4655 Msa1419590:five_prime_UTR
GGATCCAGGCATCACAACAC+CGG 0.644727 tig0024721:-7276 None:intergenic
GATATGCAAGGTTGTTGACA+GGG 0.644837 tig0024721:+6680 Msa1419590:CDS
ATACTTGTGTACGAAGGTGA+GGG 0.646011 tig0024721:+7125 Msa1419590:CDS
AGGATGTTCTTATATCCCAA+GGG 0.646556 tig0024721:+6818 Msa1419590:CDS
GTTTGAGGAGGTGAACAAAG+AGG 0.649304 tig0024721:+6707 Msa1419590:CDS
GCTCGAATTCCAATGGCAAG+AGG 0.649760 tig0024721:-6999 None:intergenic
TGGTTGATGATGGTCATGGT+TGG 0.651359 tig0024721:+7339 Msa1419590:CDS
CAAACGACCGATAAGAAATG+TGG 0.653357 tig0024721:+6191 Msa1419590:CDS
TGACAGTGAAACAACTGGGG+AGG 0.653527 tig0024721:+5744 Msa1419590:CDS
ACTCCACCAGAAACATTGCA+AGG 0.653765 tig0024721:-6143 None:intergenic
GCCTGGATCCCGTATACCTG+TGG 0.656774 tig0024721:+7289 Msa1419590:CDS
TCTAATTAATACAAACAACA+GGG 0.657712 tig0024721:-4496 Msa1419580:intron
CCAATGGTTGATGATGGTCA+TGG 0.660629 tig0024721:+7335 Msa1419590:CDS
ATGTTCTTATATCCCAAGGG+TGG 0.662483 tig0024721:+6821 Msa1419590:CDS
TGACTGGTAACTCCTCCAGA+AGG 0.672347 tig0024721:-5922 None:intergenic
GAGGGTAACCAAATCCATTG+TGG 0.675212 tig0024721:-7393 None:intergenic
GATGCCTGAACCAACAGCAG+TGG 0.675835 tig0024721:+6302 Msa1419590:CDS
CAGAGTTGATACTGACCCTG+TGG 0.678557 tig0024721:+6008 Msa1419590:CDS
TATTCATGATAATCAAACTG+AGG 0.679559 tig0024721:+7097 Msa1419590:CDS
GCTGTGACTGCTACCGCCGG+GGG 0.681642 tig0024721:+6432 Msa1419590:CDS
AAATATATTCAAGCGTCATG+GGG 0.682975 tig0024721:+6545 Msa1419590:CDS
CACAATGTAGTGTTGCTGTG+TGG 0.686328 tig0024721:+5818 Msa1419590:CDS
AGATGGTTTGAAGATATGCA+AGG 0.687926 tig0024721:+6668 Msa1419590:CDS
TTGGCAGGAAGTACCATCGG+TGG 0.690515 tig0024721:+6474 Msa1419590:CDS
ATGCAAGGTTGTTGACAGGG+CGG 0.708122 tig0024721:+6683 Msa1419590:CDS
TGTGTGGAATGGCTCCGAAG+TGG 0.718800 tig0024721:+5834 Msa1419590:CDS
TATGGAAGTGAGGATGCCAA+TGG 0.723950 tig0024721:+7319 Msa1419590:CDS
ATCTGACAGTGAAACAACTG+GGG 0.741112 tig0024721:+5741 Msa1419590:CDS
TGAGTGAGTGAGTGAGTGAG+CGG 0.748330 tig0024721:-4653 Msa1419580:intron
GCATTGATACTTGTGTACGA+AGG 0.792141 tig0024721:+7119 Msa1419590:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAAAAAGATAATAACTAAA+TGG - tig0024721:4793-4812 None:intergenic 10.0%
!! TTAAAACATTAAAAAAATCA+GGG - tig0024721:4976-4995 None:intergenic 10.0%
!!! TTTAAAACATTAAAAAAATC+AGG - tig0024721:4977-4996 None:intergenic 10.0%
!!! ATTGTAACTTTTAAAATATA+GGG + tig0024721:5109-5128 Msa1419590:intron 10.0%
!! TTTAAAAGTTACAATCAATT+TGG - tig0024721:5104-5123 None:intergenic 15.0%
!!! GATTGTAACTTTTAAAATAT+AGG + tig0024721:5108-5127 Msa1419590:intron 15.0%
!! AGATTATAAGAAATTCAAAA+AGG - tig0024721:5198-5217 None:intergenic 15.0%
!! AAAATAACAGAAGTTTATAA+AGG + tig0024721:6867-6886 Msa1419590:CDS 15.0%
!! TCTAATTAATACAAACAACA+GGG - tig0024721:4499-4518 Msa1419580:intron 20.0%
!! TTCTAATTAATACAAACAAC+AGG - tig0024721:4500-4519 Msa1419580:intron 20.0%
!!! ATCAATTCTTCTTTTCATTA+TGG + tig0024721:4868-4887 Msa1419590:intron 20.0%
!! TTGAATTTCTTATAATCTCA+AGG + tig0024721:5200-5219 Msa1419590:intron 20.0%
!! AAATTTGAGTATGTAATGTT+TGG - tig0024721:5413-5432 None:intergenic 20.0%
!! ATGTTAATATTAAGCACTAT+TGG - tig0024721:5526-5545 None:intergenic 20.0%
!! TTCAGATATAATTACATACA+TGG - tig0024721:5569-5588 None:intergenic 20.0%
!!! TTGAATATATTTTCAGAATC+TGG - tig0024721:6537-6556 None:intergenic 20.0%
!! TTAGGATATTTCAAGATAAT+GGG + tig0024721:7173-7192 Msa1419590:CDS 20.0%
! AATATGAGAGAGAAAACAAA+CGG - tig0024721:4597-4616 Msa1419580:intron 25.0%
! TTTAAATCAACTTGCCTTAT+AGG + tig0024721:4809-4828 Msa1419590:intron 25.0%
! TGAGAGACAAATAAAAATCT+CGG - tig0024721:4924-4943 None:intergenic 25.0%
! TCAAATTCAAACAATATGCT+AGG + tig0024721:5039-5058 Msa1419590:intron 25.0%
! CAAATTCAAACAATATGCTA+GGG + tig0024721:5040-5059 Msa1419590:intron 25.0%
!!! TTGACAAGTTTAAAAGTGTT+AGG + tig0024721:5170-5189 Msa1419590:intron 25.0%
!!! TGACAAGTTTAAAAGTGTTA+GGG + tig0024721:5171-5190 Msa1419590:intron 25.0%
! ATGTTACTGTGTATAATTTG+TGG + tig0024721:5294-5313 Msa1419590:intron 25.0%
!! TGTGTATAATTTGTGGTATT+TGG + tig0024721:5301-5320 Msa1419590:intron 25.0%
! ACAGTTGTTAAAACAATTAC+AGG - tig0024721:5371-5390 None:intergenic 25.0%
!!! TGTTTTAACAACTGTGAAAA+TGG + tig0024721:5376-5395 Msa1419590:intron 25.0%
! GCTTTCTATATTAGATTGTT+CGG + tig0024721:5652-5671 Msa1419590:intron 25.0%
!! AATTGCTCTCATTTTCAATA+TGG + tig0024721:6389-6408 Msa1419590:CDS 25.0%
!! ATTGCTCTCATTTTCAATAT+GGG + tig0024721:6390-6409 Msa1419590:CDS 25.0%
! AAAATATATTCAAGCGTCAT+GGG + tig0024721:6544-6563 Msa1419590:CDS 25.0%
! GGTCAAAGAAATTAAATCAA+TGG + tig0024721:6566-6585 Msa1419590:CDS 25.0%
! ATAAATGATGTGATACTTGT+TGG + tig0024721:6795-6814 Msa1419590:CDS 25.0%
!!! AACTTCTGTTATTTTGCATA+AGG - tig0024721:6862-6881 None:intergenic 25.0%
!! TTATAAAGGTATAAACCCTT+TGG + tig0024721:6881-6900 Msa1419590:CDS 25.0%
! TATTCATGATAATCAAACTG+AGG + tig0024721:7097-7116 Msa1419590:CDS 25.0%
! GTTAGGATATTTCAAGATAA+TGG + tig0024721:7172-7191 Msa1419590:CDS 25.0%
ATATGAGAGAGAAAACAAAC+GGG - tig0024721:4596-4615 Msa1419580:intron 30.0%
TAATAACTAAATGGCACTGT+TGG - tig0024721:4784-4803 None:intergenic 30.0%
AGTAGTTACAAACACCTATA+AGG - tig0024721:4826-4845 None:intergenic 30.0%
!! TGATGATGGATCATAAACTT+AGG + tig0024721:5070-5089 Msa1419590:intron 30.0%
!! GATGATGGATCATAAACTTA+GGG + tig0024721:5071-5090 Msa1419590:intron 30.0%
! CACTTTTAAACTTGTCAAGT+TGG - tig0024721:5168-5187 None:intergenic 30.0%
ATTTGGTCAGATATGTACTT+TGG + tig0024721:5318-5337 Msa1419590:intron 30.0%
TTTAACAACTGTGAAAATGG+AGG + tig0024721:5379-5398 Msa1419590:intron 30.0%
! CAATTTTTCCTGTTAAATGC+AGG + tig0024721:5686-5705 Msa1419590:intron 30.0%
ATAGCGATTGGAATTGATAT+TGG + tig0024721:5790-5809 Msa1419590:CDS 30.0%
TTCAATATGAAACGCTTGAT+CGG + tig0024721:5985-6004 Msa1419590:CDS 30.0%
! AAGTAAAAATCTGCCATTTC+TGG + tig0024721:6038-6057 Msa1419590:CDS 30.0%
AAAGCTTCTCAAGAAACTAT+GGG + tig0024721:6351-6370 Msa1419590:CDS 30.0%
GAAAATATATTCAAGCGTCA+TGG + tig0024721:6543-6562 Msa1419590:CDS 30.0%
AAATATATTCAAGCGTCATG+GGG + tig0024721:6545-6564 Msa1419590:CDS 30.0%
CATGTAAACATTGGATGATA+AGG - tig0024721:6752-6771 None:intergenic 30.0%
TCTTGAAATATCCTAACAAG+TGG - tig0024721:7169-7188 None:intergenic 30.0%
ATCAATTTATGCCTTCTTGA+GGG - tig0024721:7414-7433 None:intergenic 30.0%
TATCAATTTATGCCTTCTTG+AGG - tig0024721:7415-7434 None:intergenic 30.0%
CATGCATGACACTTTCAATT+TGG + tig0024721:4949-4968 Msa1419590:intron 35.0%
TGGATCATAAACTTAGGGTT+AGG + tig0024721:5076-5095 Msa1419590:intron 35.0%
GGATCATAAACTTAGGGTTA+GGG + tig0024721:5077-5096 Msa1419590:intron 35.0%
! GGGACTAAATTGATAGTAAG+TGG + tig0024721:5129-5148 Msa1419590:intron 35.0%
AATCCCTGACTTTCTCAAAT+AGG - tig0024721:5227-5246 None:intergenic 35.0%
GCTGCATGTAATTATATCTG+AGG + tig0024721:5591-5610 Msa1419590:intron 35.0%
! TTTTCCTGTTAAATGCAGGA+TGG + tig0024721:5690-5709 Msa1419590:intron 35.0%
! TCAATTCCAATCGCTATTTC+AGG - tig0024721:5787-5806 None:intergenic 35.0%
!! GTTTTTCAAAAGCTCCACTT+CGG - tig0024721:5851-5870 None:intergenic 35.0%
! TTTTACTTGCATGAACCACA+GGG - tig0024721:6026-6045 None:intergenic 35.0%
! TTTTTACTTGCATGAACCAC+AGG - tig0024721:6027-6046 None:intergenic 35.0%
TTCACTAACGCAGCAATAAA+TGG - tig0024721:6090-6109 None:intergenic 35.0%
GTAAGCACCACATTTCTTAT+CGG - tig0024721:6201-6220 None:intergenic 35.0%
GAAAGCTTCTCAAGAAACTA+TGG + tig0024721:6350-6369 Msa1419590:CDS 35.0%
! AGTATCCCAGATAAAAGCAT+TGG + tig0024721:6455-6474 Msa1419590:CDS 35.0%
!! TGTTCAGGTTGATGTAGATT+TGG + tig0024721:6644-6663 Msa1419590:CDS 35.0%
!! GTTCAGGTTGATGTAGATTT+GGG + tig0024721:6645-6664 Msa1419590:CDS 35.0%
AGATGGTTTGAAGATATGCA+AGG + tig0024721:6668-6687 Msa1419590:CDS 35.0%
CCTTTGCATCATGTAAACAT+TGG - tig0024721:6761-6780 None:intergenic 35.0%
CCAATGTTTACATGATGCAA+AGG + tig0024721:6758-6777 Msa1419590:CDS 35.0%
ATGATGCAAAGGTAGAAGTT+GGG + tig0024721:6769-6788 Msa1419590:CDS 35.0%
! AATGATGTGATACTTGTTGG+AGG + tig0024721:6798-6817 Msa1419590:CDS 35.0%
AGGATGTTCTTATATCCCAA+GGG + tig0024721:6818-6837 Msa1419590:CDS 35.0%
! AAAGGTATAAACCCTTTGGA+AGG + tig0024721:6885-6904 Msa1419590:CDS 35.0%
AACAAGTCCAAGTTTCCAAA+GGG - tig0024721:6963-6982 None:intergenic 35.0%
TAACAAGTCCAAGTTTCCAA+AGG - tig0024721:6964-6983 None:intergenic 35.0%
AGTTTGTACCTGTAATTCCA+AGG + tig0024721:7033-7052 Msa1419590:CDS 35.0%
AAAAGTAGATCTTTACGTGC+TGG - tig0024721:7071-7090 None:intergenic 35.0%
ACAAGAGTAGCAGAAGAAGA+AGG - tig0024721:4694-4713 None:intergenic 40.0%
AACTAAATGGCACTGTTGGT+TGG - tig0024721:4780-4799 None:intergenic 40.0%
TGACATTTGATTGATGCAGC+AGG - tig0024721:4849-4868 None:intergenic 40.0%
GCTAGGGACTAAATTGATGA+TGG + tig0024721:5056-5075 Msa1419590:intron 40.0%
AGGACCTATTTGAGAAAGTC+AGG + tig0024721:5220-5239 Msa1419590:intron 40.0%
GGACCTATTTGAGAAAGTCA+GGG + tig0024721:5221-5240 Msa1419590:intron 40.0%
GAGAAAGTCAGGGATTATGT+TGG + tig0024721:5231-5250 Msa1419590:intron 40.0%
!! CTTATGAACAGAGCACTGTA+AGG - tig0024721:5463-5482 None:intergenic 40.0%
CTTACAGTGCTCTGTTCATA+AGG + tig0024721:5461-5480 Msa1419590:intron 40.0%
TGTTTCACTGTCAGATGCAA+CGG - tig0024721:5737-5756 None:intergenic 40.0%
CATCTGACAGTGAAACAACT+GGG + tig0024721:5740-5759 Msa1419590:CDS 40.0%
ATCTGACAGTGAAACAACTG+GGG + tig0024721:5741-5760 Msa1419590:CDS 40.0%
TATCTCCAAACAGCATCTCA+TGG - tig0024721:5960-5979 None:intergenic 40.0%
AACATTGCAAGGACTTCTTC+TGG - tig0024721:6135-6154 None:intergenic 40.0%
AGAAGTCCTTGCAATGTTTC+TGG + tig0024721:6137-6156 Msa1419590:CDS 40.0%
CAAACGACCGATAAGAAATG+TGG + tig0024721:6191-6210 Msa1419590:CDS 40.0%
AAATGTGGTGCTTACTGTTC+CGG + tig0024721:6206-6225 Msa1419590:CDS 40.0%
TGGAAACGACTGAATGAAAC+CGG - tig0024721:6228-6247 None:intergenic 40.0%
TATAATAACGCCACTGCTGT+TGG - tig0024721:6315-6334 None:intergenic 40.0%
ACAGCAGTGGCGTTATTATA+TGG + tig0024721:6315-6334 Msa1419590:CDS 40.0%
! CTCATTTTCAATATGGGTGC+TGG + tig0024721:6396-6415 Msa1419590:CDS 40.0%
! TCCTGCCAATGCTTTTATCT+GGG - tig0024721:6463-6482 None:intergenic 40.0%
! TTCCTGCCAATGCTTTTATC+TGG - tig0024721:6464-6483 None:intergenic 40.0%
AAGCTGATGAATTGCATCCT+GGG - tig0024721:6610-6629 None:intergenic 40.0%
AAAGCTGATGAATTGCATCC+TGG - tig0024721:6611-6630 None:intergenic 40.0%
ATCAACCTGAACACTGCTTT+GGG - tig0024721:6637-6656 None:intergenic 40.0%
GTTGATGTAGATTTGGGAGA+TGG + tig0024721:6651-6670 Msa1419590:CDS 40.0%
AGATATGCAAGGTTGTTGAC+AGG + tig0024721:6679-6698 Msa1419590:CDS 40.0%
GATATGCAAGGTTGTTGACA+GGG + tig0024721:6680-6699 Msa1419590:CDS 40.0%
CATGATGCAAAGGTAGAAGT+TGG + tig0024721:6768-6787 Msa1419590:CDS 40.0%
TGATGCAAAGGTAGAAGTTG+GGG + tig0024721:6770-6789 Msa1419590:CDS 40.0%
GAGGATGTTCTTATATCCCA+AGG + tig0024721:6817-6836 Msa1419590:CDS 40.0%
ATGTTCTTATATCCCAAGGG+TGG + tig0024721:6821-6840 Msa1419590:CDS 40.0%
TAACAAGATTCTCCACCCTT+GGG - tig0024721:6836-6855 None:intergenic 40.0%
! TATAAACCCTTTGGAAGGTG+CGG + tig0024721:6890-6909 Msa1419590:CDS 40.0%
! GCATTGATACTTGTGTACGA+AGG + tig0024721:7119-7138 Msa1419590:CDS 40.0%
ATACTTGTGTACGAAGGTGA+GGG + tig0024721:7125-7144 Msa1419590:CDS 40.0%
GCAGAAGAAAACCACTTGTT+AGG + tig0024721:7155-7174 Msa1419590:CDS 40.0%
! CTGATTTCAGGAACTCCTTT+TGG - tig0024721:7212-7231 None:intergenic 40.0%
TCTGCCATCCTGCATTTAAC+AGG - tig0024721:5697-5716 None:intergenic 45.0%
TCAGATGCAACGGTATATGC+AGG - tig0024721:5727-5746 None:intergenic 45.0%
GCATCTGACAGTGAAACAAC+TGG + tig0024721:5739-5758 Msa1419590:CDS 45.0%
! CCAATCGCTATTTCAGGAAG+AGG - tig0024721:5781-5800 None:intergenic 45.0%
CCTCTTCCTGAAATAGCGAT+TGG + tig0024721:5778-5797 Msa1419590:CDS 45.0%
CACAGCAACACTACATTGTG+AGG - tig0024721:5818-5837 None:intergenic 45.0%
! CACAATGTAGTGTTGCTGTG+TGG + tig0024721:5818-5837 Msa1419590:CDS 45.0%
! TGTAGTGTTGCTGTGTGGAA+TGG + tig0024721:5823-5842 Msa1419590:CDS 45.0%
TTCAAAGACGAAGCTCCTTC+TGG + tig0024721:5907-5926 Msa1419590:CDS 45.0%
AAACAGCATCTCATGGTCGT+GGG - tig0024721:5953-5972 None:intergenic 45.0%
CTGCGTTAGTGAACAATGTC+TGG + tig0024721:6097-6116 Msa1419590:CDS 45.0%
!! AGTCCTTGCAATGTTTCTGG+TGG + tig0024721:6140-6159 Msa1419590:CDS 45.0%
ACTCCACCAGAAACATTGCA+AGG - tig0024721:6146-6165 None:intergenic 45.0%
TCTCAAGAAACTATGGGCAG+TGG + tig0024721:6357-6376 Msa1419590:CDS 45.0%
!! TTTTATCTGGGATACTCCCC+CGG - tig0024721:6451-6470 None:intergenic 45.0%
!! TCCCAGATAAAAGCATTGGC+AGG + tig0024721:6459-6478 Msa1419590:CDS 45.0%
TACCATCGGTGGAGAAGATT+TGG + tig0024721:6485-6504 Msa1419590:CDS 45.0%
AACCAAATCTTCTCCACCGA+TGG - tig0024721:6490-6509 None:intergenic 45.0%
CATCAACCTGAACACTGCTT+TGG - tig0024721:6638-6657 None:intergenic 45.0%
GTTTGAGGAGGTGAACAAAG+AGG + tig0024721:6707-6726 Msa1419590:CDS 45.0%
GTAACAAGATTCTCCACCCT+TGG - tig0024721:6837-6856 None:intergenic 45.0%
! CAGTGATCCCTTTGGAAACT+TGG + tig0024721:6953-6972 Msa1419590:CDS 45.0%
AATTCCAATGGCAAGAGGAG+TGG - tig0024721:6997-7016 None:intergenic 45.0%
CCATTGGAATTCGAGCTGAT+GGG + tig0024721:7006-7025 Msa1419590:CDS 45.0%
GTGGTGTTCCTTGGAATTAC+AGG - tig0024721:7044-7063 None:intergenic 45.0%
! ATCTTTACGTGCTGGCATTG+TGG - tig0024721:7063-7082 None:intergenic 45.0%
GATACTTGTGTACGAAGGTG+AGG + tig0024721:7124-7143 Msa1419590:CDS 45.0%
TCCTGAAATCAGTGTGTGCA+TGG + tig0024721:7220-7239 Msa1419590:CDS 45.0%
!! TCCATGCACACACTGATTTC+AGG - tig0024721:7224-7243 None:intergenic 45.0%
TAACAGGCACCACAGGTATA+CGG - tig0024721:7301-7320 None:intergenic 45.0%
TATACCTGTGGTGCCTGTTA+TGG + tig0024721:7301-7320 Msa1419590:CDS 45.0%
TATGGAAGTGAGGATGCCAA+TGG + tig0024721:7319-7338 Msa1419590:CDS 45.0%
!! AGGATGCCAATGGTTGATGA+TGG + tig0024721:7329-7348 Msa1419590:CDS 45.0%
CCATGACCATCATCAACCAT+TGG - tig0024721:7338-7357 None:intergenic 45.0%
!! CCAATGGTTGATGATGGTCA+TGG + tig0024721:7335-7354 Msa1419590:CDS 45.0%
!! TGGTTGATGATGGTCATGGT+TGG + tig0024721:7339-7358 Msa1419590:CDS 45.0%
GGCACCATAGGTCCTATTTA+GGG - tig0024721:7375-7394 None:intergenic 45.0%
TGGCACCATAGGTCCTATTT+AGG - tig0024721:7376-7395 None:intergenic 45.0%
CAAATCCATTGTGGCACCAT+AGG - tig0024721:7387-7406 None:intergenic 45.0%
!! CTATGGTGCCACAATGGATT+TGG + tig0024721:7385-7404 Msa1419590:CDS 45.0%
GAGGGTAACCAAATCCATTG+TGG - tig0024721:7396-7415 None:intergenic 45.0%
! GGATTTGGTTACCCTCAAGA+AGG + tig0024721:7400-7419 Msa1419590:CDS 45.0%
!!! TTTTTTTAATGTTTTAAAAA+TGG + tig0024721:4979-4998 Msa1419590:intron 5.0%
TGAGTGAGTGAGTGAGTGAG+CGG - tig0024721:4656-4675 Msa1419580:intron 50.0%
TGACAGTGAAACAACTGGGG+AGG + tig0024721:5744-5763 Msa1419590:CDS 50.0%
AAAGACGAAGCTCCTTCTGG+AGG + tig0024721:5910-5929 Msa1419590:CDS 50.0%
TGACTGGTAACTCCTCCAGA+AGG - tig0024721:5925-5944 None:intergenic 50.0%
ATGGTCGTGGGAGAATTGAC+TGG - tig0024721:5941-5960 None:intergenic 50.0%
CAAACAGCATCTCATGGTCG+TGG - tig0024721:5954-5973 None:intergenic 50.0%
CACGACCATGAGATGCTGTT+TGG + tig0024721:5952-5971 Msa1419590:CDS 50.0%
!! CAGAGTTGATACTGACCCTG+TGG + tig0024721:6008-6027 Msa1419590:CDS 50.0%
TCCAAGGTCTGCACCAGAAA+TGG - tig0024721:6054-6073 None:intergenic 50.0%
!! AAGGACTTCTTCTGGAGTGG+TGG - tig0024721:6127-6146 None:intergenic 50.0%
! TGCAAGGACTTCTTCTGGAG+TGG - tig0024721:6130-6149 None:intergenic 50.0%
! TCGCACAAGCACGCTCTAAA+CGG - tig0024721:6257-6276 None:intergenic 50.0%
! TTTAGAGCGTGCTTGTGCGA+TGG + tig0024721:6257-6276 Msa1419590:CDS 50.0%
!! TGGCTGGTCTTCATGTTCTC+AGG + tig0024721:6277-6296 Msa1419590:CDS 50.0%
!! GCATTGGCAGGAAGTACCAT+CGG + tig0024721:6471-6490 Msa1419590:CDS 50.0%
!! ATTGCTTCGAGTTGCAACCC+AGG + tig0024721:6590-6609 Msa1419590:CDS 50.0%
TTCCACCCAAAGCAGTGTTC+AGG + tig0024721:6629-6648 Msa1419590:CDS 50.0%
! AACCTGAACACTGCTTTGGG+TGG - tig0024721:6634-6653 None:intergenic 50.0%
ATGCAAGGTTGTTGACAGGG+CGG + tig0024721:6683-6702 Msa1419590:CDS 50.0%
CACAAACCGCACCTTCCAAA+GGG - tig0024721:6899-6918 None:intergenic 50.0%
GTTTGTGGTGCAGCAATGGA+AGG + tig0024721:6912-6931 Msa1419590:CDS 50.0%
! ATGGAAGGAGCTGTTGCTTC+AGG + tig0024721:6927-6946 Msa1419590:CDS 50.0%
TCAGGCATCAGTGATCCCTT+TGG + tig0024721:6945-6964 Msa1419590:CDS 50.0%
GCTCGAATTCCAATGGCAAG+AGG - tig0024721:7002-7021 None:intergenic 50.0%
GCCATTGGAATTCGAGCTGA+TGG + tig0024721:7005-7024 Msa1419590:CDS 50.0%
CCCATCAGCTCGAATTCCAA+TGG - tig0024721:7009-7028 None:intergenic 50.0%
GTACGAAGGTGAGGGAAAGA+AGG + tig0024721:7133-7152 Msa1419590:CDS 50.0%
GGAATACCTGCAGCACCAAA+AGG + tig0024721:7194-7213 Msa1419590:CDS 50.0%
!! AACGTGCTGCGAGTTTTAGC+CGG + tig0024721:7257-7276 Msa1419590:CDS 50.0%
AACAGGCACCACAGGTATAC+GGG - tig0024721:7300-7319 None:intergenic 50.0%
ACTTCCATAACAGGCACCAC+AGG - tig0024721:7308-7327 None:intergenic 50.0%
TGGTGCCTGTTATGGAAGTG+AGG + tig0024721:7309-7328 Msa1419590:CDS 50.0%
GGCATCCTCACTTCCATAAC+AGG - tig0024721:7317-7336 None:intergenic 50.0%
GAGGCCCTAAATAGGACCTA+TGG + tig0024721:7368-7387 Msa1419590:CDS 50.0%
!! TAGGACCTATGGTGCCACAA+TGG + tig0024721:7379-7398 Msa1419590:CDS 50.0%
GCTCACTCACTCACTCACTC+AGG + tig0024721:4655-4674 Msa1419590:five_prime_UTR 55.0%
TGTGTGGAATGGCTCCGAAG+TGG + tig0024721:5834-5853 Msa1419590:CDS 55.0%
!! GCCATTTCTGGTGCAGACCT+TGG + tig0024721:6050-6069 Msa1419590:CDS 55.0%
CTGGTGCAGACCTTGGACAT+TGG + tig0024721:6057-6076 Msa1419590:CDS 55.0%
TGGACGAACGCCAATGTCCA+AGG - tig0024721:6070-6089 None:intergenic 55.0%
! CGCACAAGCACGCTCTAAAC+GGG - tig0024721:6256-6275 None:intergenic 55.0%
GATGCCTGAACCAACAGCAG+TGG + tig0024721:6302-6321 Msa1419590:CDS 55.0%
!! AACGCCACTGCTGTTGGTTC+AGG - tig0024721:6309-6328 None:intergenic 55.0%
!! TTGGCAGGAAGTACCATCGG+TGG + tig0024721:6474-6493 Msa1419590:CDS 55.0%
TGTTGACAGGGCGGAGTTTG+AGG + tig0024721:6692-6711 Msa1419590:CDS 55.0%
CCACAAACCGCACCTTCCAA+AGG - tig0024721:6900-6919 None:intergenic 55.0%
! CCTTTGGAAGGTGCGGTTTG+TGG + tig0024721:6897-6916 Msa1419590:CDS 55.0%
TGCGGTTTGTGGTGCAGCAA+TGG + tig0024721:6908-6927 Msa1419590:CDS 55.0%
CAAGCCACTCCTCTTGCCAT+TGG + tig0024721:6990-7009 Msa1419590:CDS 55.0%
GCTGGCATTGTGGTGTTCCT+TGG - tig0024721:7053-7072 None:intergenic 55.0%
!!! GGAACTCCTTTTGGTGCTGC+AGG - tig0024721:7203-7222 None:intergenic 55.0%
!! TTAGCCGGTGTTGTGATGCC+TGG + tig0024721:7272-7291 Msa1419590:CDS 55.0%
GGATCCAGGCATCACAACAC+CGG - tig0024721:7279-7298 None:intergenic 55.0%
ACCACAGGTATACGGGATCC+AGG - tig0024721:7293-7312 None:intergenic 55.0%
! TGGTCATGGTTGGTGTGCTG+AGG + tig0024721:7349-7368 Msa1419590:CDS 55.0%
GGTGTGCTGAGGCCCTAAAT+AGG + tig0024721:7360-7379 Msa1419590:CDS 55.0%
! CACGCTCTAAACGGGTCAGC+TGG - tig0024721:6248-6267 None:intergenic 60.0%
GTTGCTGTGACTGCTACCGC+CGG + tig0024721:6429-6448 Msa1419590:CDS 60.0%
TTGCTGTGACTGCTACCGCC+GGG + tig0024721:6430-6449 Msa1419590:CDS 60.0%
! TATCTGGGATACTCCCCCGG+CGG - tig0024721:6448-6467 None:intergenic 60.0%
TGACAGGGCGGAGTTTGAGG+AGG + tig0024721:6695-6714 Msa1419590:CDS 60.0%
GCCTGGATCCCGTATACCTG+TGG + tig0024721:7289-7308 Msa1419590:CDS 60.0%
GAGCGTGCTTGTGCGATGGC+TGG + tig0024721:6261-6280 Msa1419590:CDS 65.0%
TGCTGTGACTGCTACCGCCG+GGG + tig0024721:6431-6450 Msa1419590:CDS 65.0%
GCTGTGACTGCTACCGCCGG+GGG + tig0024721:6432-6451 Msa1419590:CDS 70.0%
Chromosome Type Strat End Strand Name
tig0024721 gene 4475 7451 4475 ID=Msa1419590;Name=Msa1419590
tig0024721 mRNA 4475 7451 4475 ID=Msa1419590-mRNA-1;Parent=Msa1419590;Name=Msa1419590-mRNA-1;_AED=0.00;_eAED=0.00;_QI=202|0|0.5|1|0|0.5|2|24|572
tig0024721 exon 4475 4676 4475 ID=Msa1419590-mRNA-1:exon:994;Parent=Msa1419590-mRNA-1
tig0024721 exon 5709 7451 5709 ID=Msa1419590-mRNA-1:exon:995;Parent=Msa1419590-mRNA-1
tig0024721 five_prime_UTR 4475 4676 4475 ID=Msa1419590-mRNA-1:five_prime_utr;Parent=Msa1419590-mRNA-1
tig0024721 CDS 5709 7427 5709 ID=Msa1419590-mRNA-1:cds;Parent=Msa1419590-mRNA-1
tig0024721 three_prime_UTR 7428 7451 7428 ID=Msa1419590-mRNA-1:three_prime_utr;Parent=Msa1419590-mRNA-1
Gene Sequence

>Msa1419590

ATGGCAGAACGAGAACCTGCATATACCGTTGCATCTGACAGTGAAACAACTGGGGAGGAAAAATCATCACCTCTTCCTGAAATAGCGATTGGAATTGATATTGGCACCTCACAATGTAGTGTTGCTGTGTGGAATGGCTCCGAAGTGGAGCTTTTGAAAAACAAAAGAAATCAGAAGCTTATGAAGTCATTTGTGACTTTCAAAGACGAAGCTCCTTCTGGAGGAGTTACCAGTCAATTCTCCCACGACCATGAGATGCTGTTTGGAGATACAATTTTCAATATGAAACGCTTGATCGGCAGAGTTGATACTGACCCTGTGGTTCATGCAAGTAAAAATCTGCCATTTCTGGTGCAGACCTTGGACATTGGCGTTCGTCCATTTATTGCTGCGTTAGTGAACAATGTCTGGAGATCCACCACTCCAGAAGAAGTCCTTGCAATGTTTCTGGTGGAGTTAAGACTGATGACTGAAACTCATCTCAAACGACCGATAAGAAATGTGGTGCTTACTGTTCCGGTTTCATTCAGTCGTTTCCAGCTGACCCGTTTAGAGCGTGCTTGTGCGATGGCTGGTCTTCATGTTCTCAGGTTGATGCCTGAACCAACAGCAGTGGCGTTATTATATGGACAACAACAACAGAAAGCTTCTCAAGAAACTATGGGCAGTGGAAGTGAGAAAATTGCTCTCATTTTCAATATGGGTGCTGGTTATTGTGATGTTGCTGTGACTGCTACCGCCGGGGGAGTATCCCAGATAAAAGCATTGGCAGGAAGTACCATCGGTGGAGAAGATTTGGTTCAGAATATGATGTGTTATCTCTTACCAGATTCTGAAAATATATTCAAGCGTCATGGGGTCAAAGAAATTAAATCAATGGCATTGCTTCGAGTTGCAACCCAGGATGCAATTCATCAGCTTTCCACCCAAAGCAGTGTTCAGGTTGATGTAGATTTGGGAGATGGTTTGAAGATATGCAAGGTTGTTGACAGGGCGGAGTTTGAGGAGGTGAACAAAGAGGTGTTCGAAAAGTGTGAAAGCCTTATCATCCAATGTTTACATGATGCAAAGGTAGAAGTTGGGGATATAAATGATGTGATACTTGTTGGAGGATGTTCTTATATCCCAAGGGTGGAGAATCTTGTTACAACCTTATGCAAAATAACAGAAGTTTATAAAGGTATAAACCCTTTGGAAGGTGCGGTTTGTGGTGCAGCAATGGAAGGAGCTGTTGCTTCAGGCATCAGTGATCCCTTTGGAAACTTGGACTTGTTAACGATTCAAGCCACTCCTCTTGCCATTGGAATTCGAGCTGATGGGAACAAGTTTGTACCTGTAATTCCAAGGAACACCACAATGCCAGCACGTAAAGATCTACTTTTCACAACTATTCATGATAATCAAACTGAGGCATTGATACTTGTGTACGAAGGTGAGGGAAAGAAGGCAGAAGAAAACCACTTGTTAGGATATTTCAAGATAATGGGAATACCTGCAGCACCAAAAGGAGTTCCTGAAATCAGTGTGTGCATGGACATAGATGCTGCAAACGTGCTGCGAGTTTTAGCCGGTGTTGTGATGCCTGGATCCCGTATACCTGTGGTGCCTGTTATGGAAGTGAGGATGCCAATGGTTGATGATGGTCATGGTTGGTGTGCTGAGGCCCTAAATAGGACCTATGGTGCCACAATGGATTTGGTTACCCTCAAGAAGGCATAA
Protein sequence

>Msa1419590

MAEREPAYTVASDSETTGEEKSSPLPEIAIGIDIGTSQCSVAVWNGSEVELLKNKRNQKLMKSFVTFKDEAPSGGVTSQFSHDHEMLFGDTIFNMKRLIGRVDTDPVVHASKNLPFLVQTLDIGVRPFIAALVNNVWRSTTPEEVLAMFLVELRLMTETHLKRPIRNVVLTVPVSFSRFQLTRLERACAMAGLHVLRLMPEPTAVALLYGQQQQKASQETMGSGSEKIALIFNMGAGYCDVAVTATAGGVSQIKALAGSTIGGEDLVQNMMCYLLPDSENIFKRHGVKEIKSMALLRVATQDAIHQLSTQSSVQVDVDLGDGLKICKVVDRAEFEEVNKEVFEKCESLIIQCLHDAKVEVGDINDVILVGGCSYIPRVENLVTTLCKITEVYKGINPLEGAVCGAAMEGAVASGISDPFGNLDLLTIQATPLAIGIRADGNKFVPVIPRNTTMPARKDLLFTTIHDNQTEALILVYEGEGKKAEENHLLGYFKIMGIPAAPKGVPEISVCMDIDAANVLRVLAGVVMPGSRIPVVPVMEVRMPMVDDGHGWCAEALNRTYGATMDLVTLKKA*