AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1135900

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1135900 MtrunA17_Chr7g0217141 98.925 93 1 0 1 93 1 93 7.14e-60 177
Msa1135900 MtrunA17_Chr6g0453041 84.615 78 12 0 16 93 15 92 3.81e-40 127
Msa1135900 MtrunA17_Chr8g0362131 73.494 83 21 1 9 91 10 91 9.25e-34 111
Msa1135900 MtrunA17_Chr3g0093001 74.359 78 18 1 16 93 17 92 1.16e-32 108
Msa1135900 MtrunA17_Chr5g0416431 69.737 76 23 0 16 91 17 92 1.09e-31 106
Msa1135900 MtrunA17_Chr7g0237771 45.205 73 40 0 6 78 2 74 1.40e-11 55.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1135900 AT3G28857.1 72.826 92 23 2 1 91 1 91 2.42e-37 121
Msa1135900 AT1G26945.1 77.333 75 17 0 18 92 19 93 3.63e-34 113
Msa1135900 AT5G39860.1 71.250 80 22 1 13 91 12 91 2.57e-32 108
Msa1135900 AT3G47710.1 62.766 94 32 2 1 93 1 92 3.37e-31 105
Msa1135900 AT5G15160.1 69.737 76 22 1 18 92 18 93 4.58e-30 102
Msa1135900 AT1G74500.1 68.354 79 22 2 16 93 17 93 7.27e-29 99.8

Find 53 sgRNAs with CRISPR-Local

Find 220 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCACTAATTTAGCTTAATTA+AGG 0.234688 7_4:+3723387 Msa1135900:five_prime_UTR
TGCAAACAAGGTCGATGATT+TGG 0.301526 7_4:-3723468 None:intergenic
ATTCAATTGATTTGAGAGTT+TGG 0.303158 7_4:+3723021 Msa1135900:five_prime_UTR
TAAGTTTCTGATATAGTTGC+AGG 0.316132 7_4:-3726434 None:intergenic
AACATTTGGCCAATTAGCTT+TGG 0.337798 7_4:-3723101 None:intergenic
CATGCAGGTACCAGCTTCTA+AGG 0.347667 7_4:+3726399 Msa1135900:intron
GAAGTGTAAGATATGATATA+TGG 0.371093 7_4:-3723228 None:intergenic
TATAGTTGCAGGTTTCTTGT+AGG 0.377852 7_4:-3726423 None:intergenic
AGAAACTATTAATTCATCAT+TGG 0.386959 7_4:-3726555 None:intergenic
TCTGAGATTGTCTCAGTTGT+TGG 0.390220 7_4:+3726480 Msa1135900:CDS
TAATTCATCATTGGTTAAGT+AGG 0.403741 7_4:-3726546 None:intergenic
TTAAGCTAAATTAGTGCAAT+GGG 0.405387 7_4:-3723381 None:intergenic
GTGCTGAAGCTTCCATCATT+AGG 0.408599 7_4:+3726521 Msa1135900:CDS
TTGGAAGGGAAGAAAACATT+TGG 0.426921 7_4:-3723115 None:intergenic
CTTTGGAAAAGGAGTGGACT+AGG 0.431847 7_4:-3723084 None:intergenic
TTGTTCCTGAGATTCGTAAC+AGG 0.433720 7_4:+3723503 Msa1135900:CDS
AAAGTGGTGAAAGAGGTATA+AGG 0.458303 7_4:-3723190 None:intergenic
TAATGACTTCAGTTGGATAG+AGG 0.463225 7_4:+3726730 Msa1135900:three_prime_UTR
ATTAAGCTAAATTAGTGCAA+TGG 0.469279 7_4:-3723382 None:intergenic
TGGCCAATTAGCTTTGGAAA+AGG 0.486700 7_4:-3723095 None:intergenic
GACACAAGCTATATATATAG+TGG 0.488522 7_4:-3723276 None:intergenic
CGAAGGTCAAGGCAACAATC+AGG 0.491771 7_4:+3723421 Msa1135900:CDS
GTTAAGTAGGCTCCTAATGA+TGG 0.500773 7_4:-3726533 None:intergenic
TATATATAGTGGAAGGGGAA+TGG 0.503862 7_4:-3723265 None:intergenic
AAGAGGTATAAGGAAGGAAA+AGG 0.508895 7_4:-3723180 None:intergenic
GAGCGCCTGTTACGAATCTC+AGG 0.511807 7_4:-3723508 None:intergenic
AATGGAGAAAGTGGTGAAAG+AGG 0.523059 7_4:-3723197 None:intergenic
TCATTATATATGTTATAGCT+AGG 0.523956 7_4:+3723326 Msa1135900:five_prime_UTR
TCTTGTAGGACCTTAGAAGC+TGG 0.539736 7_4:-3726409 None:intergenic
TTACTGTACTCTCTCTATTG+TGG 0.542121 7_4:+3723152 Msa1135900:five_prime_UTR
AACAATCAGGAGGGTCTACT+CGG 0.547851 7_4:+3723434 Msa1135900:CDS
CTCCCTAAAATTCACACCTA+GGG 0.548766 7_4:+3723057 Msa1135900:five_prime_UTR
AGCTATATATATAGTGGAAG+GGG 0.549770 7_4:-3723270 None:intergenic
TATAGTGGAAGGGGAATGGA+AGG 0.551095 7_4:-3723261 None:intergenic
CAAGCTATATATATAGTGGA+AGG 0.558395 7_4:-3723272 None:intergenic
AGTAAAGTAGACAACAATGT+TGG 0.560866 7_4:-3723134 None:intergenic
CCTCCCTAAAATTCACACCT+AGG 0.566541 7_4:+3723056 Msa1135900:five_prime_UTR
AAGCTATATATATAGTGGAA+GGG 0.569743 7_4:-3723271 None:intergenic
AAGTAGACAACAATGTTGGA+AGG 0.575963 7_4:-3723130 None:intergenic
GATAGTGTAATGACTTCAGT+TGG 0.579731 7_4:+3726723 Msa1135900:three_prime_UTR
GTTGACGCAATTTGCAAACA+AGG 0.588186 7_4:-3723480 None:intergenic
TCGTAACAGGCGCTCTGACA+AGG 0.592126 7_4:+3723516 Msa1135900:CDS
GAGTGGACTAGGCTTTCCCT+AGG 0.592349 7_4:-3723073 None:intergenic
TGGTGAAAGAGGTATAAGGA+AGG 0.597415 7_4:-3723186 None:intergenic
AATTAGCTTTGGAAAAGGAG+TGG 0.597808 7_4:-3723090 None:intergenic
AGTAGACAACAATGTTGGAA+GGG 0.605803 7_4:-3723129 None:intergenic
TATCAGAAACTTACAAAGTG+AGG 0.615011 7_4:+3726444 Msa1135900:CDS
GGTATAAGGAAGGAAAAGGA+TGG 0.615207 7_4:-3723176 None:intergenic
ATATGTCTAGTCGAAGGTCA+AGG 0.620989 7_4:+3723410 Msa1135900:exon
AGGTCAAGGCAACAATCAGG+AGG 0.632611 7_4:+3723424 Msa1135900:CDS
TTAAGGATATGTCTAGTCGA+AGG 0.649414 7_4:+3723404 Msa1135900:exon
AGCACTATCAGAATCAATTG+TGG 0.660250 7_4:-3726503 None:intergenic
GGTCAAGGCAACAATCAGGA+GGG 0.687654 7_4:+3723425 Msa1135900:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATATATATATATATATAT+AGG + chr7_4:3724486-3724505 Msa1135900:intron 0.0%
!! TATATATATATATATATATA+GGG + chr7_4:3724485-3724504 Msa1135900:intron 0.0%
!! ATATATATATATATATATAT+GGG - chr7_4:3724486-3724505 None:intergenic 0.0%
!! TATATATATATATATATATA+TGG - chr7_4:3724485-3724504 None:intergenic 0.0%
!! TATATATATATATATATATA+TGG - chr7_4:3724485-3724504 None:intergenic 0.0%
!! TAAGAAAAATGTATAAATAA+AGG + chr7_4:3723736-3723755 Msa1135900:intron 10.0%
!! AAGAAAAATGTATAAATAAA+GGG + chr7_4:3723737-3723756 Msa1135900:intron 10.0%
!!! TTAATAAGAAAATCTTTTAT+GGG - chr7_4:3724164-3724183 None:intergenic 10.0%
!!! TATAATAGTTTTTTTTTTGA+AGG - chr7_4:3724375-3724394 None:intergenic 10.0%
!! ATATATATATATTAGTTCAT+AGG + chr7_4:3724890-3724909 Msa1135900:intron 10.0%
!! TTAAAATGTCATATAAAAAA+GGG - chr7_4:3725883-3725902 None:intergenic 10.0%
!!! TTTAAAATGTCATATAAAAA+AGG - chr7_4:3725884-3725903 None:intergenic 10.0%
!!! ATAATTATTTTAGTATTTAG+AGG - chr7_4:3725942-3725961 None:intergenic 10.0%
!!! TACTTCAAATATTTTGAATT+AGG - chr7_4:3723617-3723636 None:intergenic 15.0%
!!! ATCAAGATAGTATTATTTTA+GGG - chr7_4:3723645-3723664 None:intergenic 15.0%
!! AAAATTGTGTCAATTAAATT+GGG - chr7_4:3723701-3723720 None:intergenic 15.0%
!! AGAAAAATGTATAAATAAAG+GGG + chr7_4:3723738-3723757 Msa1135900:intron 15.0%
!! AAAGTGAAATATATTGTATT+GGG + chr7_4:3723761-3723780 Msa1135900:intron 15.0%
!! TATTTAGTGTTATATTGTAT+TGG + chr7_4:3723801-3723820 Msa1135900:intron 15.0%
!! ATTTAGTGTTATATTGTATT+GGG + chr7_4:3723802-3723821 Msa1135900:intron 15.0%
!!! AAAACTTGCTAAAAATATAA+TGG + chr7_4:3723986-3724005 Msa1135900:intron 15.0%
!! AACATTGAAAATTATAGTTA+CGG - chr7_4:3724050-3724069 None:intergenic 15.0%
!!! CTTAATAAGAAAATCTTTTA+TGG - chr7_4:3724165-3724184 None:intergenic 15.0%
!!! CATTGGTTATTTATTTATTT+TGG - chr7_4:3724410-3724429 None:intergenic 15.0%
!!! AATAAATAAGGGTATAATAA+TGG - chr7_4:3725055-3725074 None:intergenic 15.0%
!! GAATGAAATTCAATAAATAA+GGG - chr7_4:3725066-3725085 None:intergenic 15.0%
!! TGAATGAAATTCAATAAATA+AGG - chr7_4:3725067-3725086 None:intergenic 15.0%
!!! CATATATATCTTCATTTTTA+AGG - chr7_4:3725777-3725796 None:intergenic 15.0%
!! TTCTATATAACTTTACTATT+TGG - chr7_4:3725970-3725989 None:intergenic 15.0%
!! ATATGAAATTAATTAAAGTG+TGG - chr7_4:3726334-3726353 None:intergenic 15.0%
!!! TTTGTTTTTGAGTTTAATTT+TGG + chr7_4:3726803-3726822 Msa1135900:three_prime_UTR 15.0%
!! TCATTATATATGTTATAGCT+AGG + chr7_4:3723326-3723345 Msa1135900:five_prime_UTR 20.0%
!!! CATCAAGATAGTATTATTTT+AGG - chr7_4:3723646-3723665 None:intergenic 20.0%
!! CAAAATTGTGTCAATTAAAT+TGG - chr7_4:3723702-3723721 None:intergenic 20.0%
!! GAAAGTGAAATATATTGTAT+TGG + chr7_4:3723760-3723779 Msa1135900:intron 20.0%
!! AGTTGTATTGAAAATTGAAA+TGG + chr7_4:3723834-3723853 Msa1135900:intron 20.0%
!! GTTGTATTGAAAATTGAAAT+GGG + chr7_4:3723835-3723854 Msa1135900:intron 20.0%
!!! GACACTTTTTTTTAGAAAAT+GGG + chr7_4:3723871-3723890 Msa1135900:intron 20.0%
!! ATTAATAACCAATGATAGTA+AGG + chr7_4:3724264-3724283 Msa1135900:intron 20.0%
!! TTAATAACCAATGATAGTAA+GGG + chr7_4:3724265-3724284 Msa1135900:intron 20.0%
!! AAATAAATAACCAATGAGAA+TGG + chr7_4:3724414-3724433 Msa1135900:intron 20.0%
!! AAGTTAATAAATACACCTTA+AGG + chr7_4:3724602-3724621 Msa1135900:intron 20.0%
!! TATATAACTTTACTATTTGG+AGG - chr7_4:3725967-3725986 None:intergenic 20.0%
!!! AAAAAAAAATTGGATTAGTC+TGG - chr7_4:3726133-3726152 None:intergenic 20.0%
!! CGATAATAATAAAGATCAAA+TGG + chr7_4:3726188-3726207 Msa1135900:intron 20.0%
!! TTAATTTCATATGCAAAGTA+CGG + chr7_4:3726342-3726361 Msa1135900:intron 20.0%
!! AGAAACTATTAATTCATCAT+TGG - chr7_4:3726558-3726577 None:intergenic 20.0%
!!! ATTCAATTGATTTGAGAGTT+TGG + chr7_4:3723021-3723040 Msa1135900:five_prime_UTR 25.0%
! TGATATATGGTATGCAAAAA+TGG - chr7_4:3723218-3723237 None:intergenic 25.0%
! GAAGTGTAAGATATGATATA+TGG - chr7_4:3723231-3723250 None:intergenic 25.0%
!! AAGCTATATATATAGTGGAA+GGG - chr7_4:3723274-3723293 None:intergenic 25.0%
! TTAAGCTAAATTAGTGCAAT+GGG - chr7_4:3723384-3723403 None:intergenic 25.0%
! ATTAAGCTAAATTAGTGCAA+TGG - chr7_4:3723385-3723404 None:intergenic 25.0%
!! GCACTAATTTAGCTTAATTA+AGG + chr7_4:3723387-3723406 Msa1135900:five_prime_UTR 25.0%
! ATAGATACACACTATTCATA+AGG + chr7_4:3723563-3723582 Msa1135900:intron 25.0%
! AATAATACTATCTTGATGTG+TGG + chr7_4:3723648-3723667 Msa1135900:intron 25.0%
!!! GGACACTTTTTTTTAGAAAA+TGG + chr7_4:3723870-3723889 Msa1135900:intron 25.0%
! AAAATATAATGGTTCACTGT+TGG + chr7_4:3723997-3724016 Msa1135900:intron 25.0%
! TTGAAAATTATAGTTACGGT+AGG - chr7_4:3724046-3724065 None:intergenic 25.0%
! TAATAACCAATGATAGTAAG+GGG + chr7_4:3724266-3724285 Msa1135900:intron 25.0%
! AAAAGTAATAAATGCTGCAT+TGG - chr7_4:3725029-3725048 None:intergenic 25.0%
! TCCACTAACTACAATTAATA+AGG + chr7_4:3725097-3725116 Msa1135900:intron 25.0%
! CCACTAACTACAATTAATAA+GGG + chr7_4:3725098-3725117 Msa1135900:intron 25.0%
!!! ATTGATTGTGTTGATTTCAA+TGG - chr7_4:3725150-3725169 None:intergenic 25.0%
!! CTCTAAATTATTGTCGTTTA+AGG - chr7_4:3725198-3725217 None:intergenic 25.0%
! AAACGACAATAATTTAGAGA+CGG + chr7_4:3725199-3725218 Msa1135900:intron 25.0%
!! TGATATGTGCTGTATTTTAT+TGG - chr7_4:3725260-3725279 None:intergenic 25.0%
!!! CATTGGCTTATAAAGTTATA+AGG + chr7_4:3725312-3725331 Msa1135900:intron 25.0%
! TAAGCAATAGAGTAAGATAA+CGG + chr7_4:3725451-3725470 Msa1135900:intron 25.0%
!!! GAATCTTTTTTGAGGAATAA+TGG + chr7_4:3725477-3725496 Msa1135900:intron 25.0%
! AATTTCTTCTTCAATACATG+GGG - chr7_4:3725645-3725664 None:intergenic 25.0%
! GAATTTCTTCTTCAATACAT+GGG - chr7_4:3725646-3725665 None:intergenic 25.0%
!!! AGATTTTGGTATTGTACTTT+TGG - chr7_4:3725668-3725687 None:intergenic 25.0%
!! AAAGTTATGGACATAGATTT+TGG - chr7_4:3725682-3725701 None:intergenic 25.0%
! ATCTATGTCCATAACTTTAA+AGG + chr7_4:3725684-3725703 Msa1135900:intron 25.0%
! TCTATGTCCATAACTTTAAA+GGG + chr7_4:3725685-3725704 Msa1135900:intron 25.0%
!!! ATGTAAACCCTTTAAAGTTA+TGG - chr7_4:3725695-3725714 None:intergenic 25.0%
! TTTAAAGGGTTTACATTGAT+TGG + chr7_4:3725699-3725718 Msa1135900:intron 25.0%
! TCTCGACGAAAAAAAAAAAT+TGG - chr7_4:3726143-3726162 None:intergenic 25.0%
!! ATGCACTATGCATTTTTATT+TGG - chr7_4:3726238-3726257 None:intergenic 25.0%
!! ATTGTTACCAACATGTTTTT+AGG + chr7_4:3726262-3726281 Msa1135900:intron 25.0%
!! TAATTCATCATTGGTTAAGT+AGG - chr7_4:3726549-3726568 None:intergenic 25.0%
AGTAAAGTAGACAACAATGT+TGG - chr7_4:3723137-3723156 None:intergenic 30.0%
! AGCTATATATATAGTGGAAG+GGG - chr7_4:3723273-3723292 None:intergenic 30.0%
! CAAGCTATATATATAGTGGA+AGG - chr7_4:3723275-3723294 None:intergenic 30.0%
GACACAAGCTATATATATAG+TGG - chr7_4:3723279-3723298 None:intergenic 30.0%
ACACACTATTCATAAGGATA+AGG + chr7_4:3723569-3723588 Msa1135900:intron 30.0%
TTGTGTCAATTAAATTGGGA+CGG - chr7_4:3723697-3723716 None:intergenic 30.0%
! TTGAAATGGGTCAGTTATTT+TGG + chr7_4:3723848-3723867 Msa1135900:intron 30.0%
!! TGAAATGGGTCAGTTATTTT+GGG + chr7_4:3723849-3723868 Msa1135900:intron 30.0%
AAAAATCCATCTATAGACCA+TGG - chr7_4:3723955-3723974 None:intergenic 30.0%
ATTTAGTGTATGCTTGCATA+TGG + chr7_4:3724102-3724121 Msa1135900:intron 30.0%
ATATGGTTAACCAAATGTTG+CGG + chr7_4:3724119-3724138 Msa1135900:intron 30.0%
TTATAGCAACATACACCTTA+AGG - chr7_4:3724620-3724639 None:intergenic 30.0%
ACCTTCTAACAAAAACAAGT+GGG + chr7_4:3724643-3724662 Msa1135900:intron 30.0%
! ACTCCTTCTAAAAAGTAAGT+GGG - chr7_4:3724708-3724727 None:intergenic 30.0%
! TACTCCTTCTAAAAAGTAAG+TGG - chr7_4:3724709-3724728 None:intergenic 30.0%
GCAAGTGAATAACTAAAAAG+TGG + chr7_4:3724734-3724753 Msa1135900:intron 30.0%
AAGAGACTATTATTGTGAGA+CGG - chr7_4:3724963-3724982 None:intergenic 30.0%
CCCTTATTAATTGTAGTTAG+TGG - chr7_4:3725101-3725120 None:intergenic 30.0%
ATACAGCACATATCAAACTT+AGG + chr7_4:3725265-3725284 Msa1135900:intron 30.0%
!!! AACGGTATGAATCTTTTTTG+AGG + chr7_4:3725469-3725488 Msa1135900:intron 30.0%
GGAATTTCTTCTTCAATACA+TGG - chr7_4:3725647-3725666 None:intergenic 30.0%
TTCAAGACGGTTTATATGTA+AGG - chr7_4:3725848-3725867 None:intergenic 30.0%
GACAAGAATAAAGTTCAAGA+CGG - chr7_4:3725861-3725880 None:intergenic 30.0%
TCAATTTCGATGGTTTAGTT+TGG - chr7_4:3726045-3726064 None:intergenic 30.0%
TAGTCTGGTGATATTTACTT+GGG - chr7_4:3726118-3726137 None:intergenic 30.0%
TTAGTCTGGTGATATTTACT+TGG - chr7_4:3726119-3726138 None:intergenic 30.0%
TAAGTTTCTGATATAGTTGC+AGG - chr7_4:3726437-3726456 None:intergenic 30.0%
TATCAGAAACTTACAAAGTG+AGG + chr7_4:3726444-3726463 Msa1135900:CDS 30.0%
! TAATATGTGCCTCTTTTTGA+AGG + chr7_4:3726654-3726673 Msa1135900:three_prime_UTR 30.0%
! TCAAACAAGTGCTACATTTT+TGG - chr7_4:3726680-3726699 None:intergenic 30.0%
! TTCCCTAGGTGTGAATTTTA+GGG - chr7_4:3723062-3723081 None:intergenic 35.0%
! TTTCCCTAGGTGTGAATTTT+AGG - chr7_4:3723063-3723082 None:intergenic 35.0%
!! AATTAGCTTTGGAAAAGGAG+TGG - chr7_4:3723093-3723112 None:intergenic 35.0%
! ACTCCTTTTCCAAAGCTAAT+TGG + chr7_4:3723092-3723111 Msa1135900:five_prime_UTR 35.0%
! AACATTTGGCCAATTAGCTT+TGG - chr7_4:3723104-3723123 None:intergenic 35.0%
TTGGAAGGGAAGAAAACATT+TGG - chr7_4:3723118-3723137 None:intergenic 35.0%
AGTAGACAACAATGTTGGAA+GGG - chr7_4:3723132-3723151 None:intergenic 35.0%
AAGTAGACAACAATGTTGGA+AGG - chr7_4:3723133-3723152 None:intergenic 35.0%
TTACTGTACTCTCTCTATTG+TGG + chr7_4:3723152-3723171 Msa1135900:five_prime_UTR 35.0%
! AAGAGGTATAAGGAAGGAAA+AGG - chr7_4:3723183-3723202 None:intergenic 35.0%
! AAAGTGGTGAAAGAGGTATA+AGG - chr7_4:3723193-3723212 None:intergenic 35.0%
GTATGCAAAAATGGAGAAAG+TGG - chr7_4:3723209-3723228 None:intergenic 35.0%
! TATATATAGTGGAAGGGGAA+TGG - chr7_4:3723268-3723287 None:intergenic 35.0%
TTAAGGATATGTCTAGTCGA+AGG + chr7_4:3723404-3723423 Msa1135900:exon 35.0%
! ACGGAGGTAGTATCATTTTA+GGG - chr7_4:3723678-3723697 None:intergenic 35.0%
AGAAAATGGGTCAGTTATTC+CGG + chr7_4:3723884-3723903 Msa1135900:intron 35.0%
ATCTATAGACCATGGTTTAG+AGG - chr7_4:3723947-3723966 None:intergenic 35.0%
TCTAAACCATGGTCTATAGA+TGG + chr7_4:3723946-3723965 Msa1135900:intron 35.0%
CGGTAGGTCATTATCTAATA+TGG - chr7_4:3724030-3724049 None:intergenic 35.0%
TTTCATACATCCGCAACATT+TGG - chr7_4:3724132-3724151 None:intergenic 35.0%
ACAATGTTGACCATTCTCAT+TGG - chr7_4:3724427-3724446 None:intergenic 35.0%
!!! ACACCCACTAGTTTTTATGT+GGG + chr7_4:3724554-3724573 Msa1135900:intron 35.0%
ACTCCCACATAAAAACTAGT+GGG - chr7_4:3724791-3724810 None:intergenic 35.0%
CACCTTCTAACAAAAACAAG+TGG + chr7_4:3724642-3724661 Msa1135900:intron 35.0%
!!! ACCCACTTGTTTTTGTTAGA+AGG - chr7_4:3724647-3724666 None:intergenic 35.0%
TGTGTTCTAGCAAATCCTAT+AGG + chr7_4:3724666-3724685 Msa1135900:intron 35.0%
GTGTTCTAGCAAATCCTATA+GGG + chr7_4:3724667-3724686 Msa1135900:intron 35.0%
TGTTCTAGCAAATCCTATAG+GGG + chr7_4:3724668-3724687 Msa1135900:intron 35.0%
!!! ACACCCACTTACTTTTTAGA+AGG + chr7_4:3724702-3724721 Msa1135900:intron 35.0%
AAGTGGTTAAATACACCCTA+AGG + chr7_4:3724751-3724770 Msa1135900:intron 35.0%
TTTAGCAAAGCTACACCTTA+GGG - chr7_4:3724769-3724788 None:intergenic 35.0%
! TTTTAGCAAAGCTACACCTT+AGG - chr7_4:3724770-3724789 None:intergenic 35.0%
ACTCCCACATAAAAACTAGT+GGG + chr7_4:3724791-3724810 Msa1135900:intron 35.0%
!!! ACACCCACTAGTTTTTATGT+GGG - chr7_4:3724554-3724573 None:intergenic 35.0%
GACAATAATTTAGAGACGGA+GGG + chr7_4:3725203-3725222 Msa1135900:intron 35.0%
TATCAAACTTAGGCTGAAGT+TGG + chr7_4:3725275-3725294 Msa1135900:intron 35.0%
TATCACCTAGTTTCTTCAAG+AGG - chr7_4:3725341-3725360 None:intergenic 35.0%
TTCGTCGAGATTCAATACAT+AGG + chr7_4:3726153-3726172 Msa1135900:intron 35.0%
TCGTCGAGATTCAATACATA+GGG + chr7_4:3726154-3726173 Msa1135900:intron 35.0%
! TTTATTTGGAGTCCATGAGT+CGG - chr7_4:3726224-3726243 None:intergenic 35.0%
ATGGTCACCTAAAAACATGT+TGG - chr7_4:3726272-3726291 None:intergenic 35.0%
GAGAGAAAAAACATCACACA+TGG - chr7_4:3726291-3726310 None:intergenic 35.0%
TATAGTTGCAGGTTTCTTGT+AGG - chr7_4:3726426-3726445 None:intergenic 35.0%
AGCACTATCAGAATCAATTG+TGG - chr7_4:3726506-3726525 None:intergenic 35.0%
!!! CATTTTTGGCCTTCAAAAAG+AGG - chr7_4:3726666-3726685 None:intergenic 35.0%
GATAGTGTAATGACTTCAGT+TGG + chr7_4:3726723-3726742 Msa1135900:three_prime_UTR 35.0%
TAATGACTTCAGTTGGATAG+AGG + chr7_4:3726730-3726749 Msa1135900:three_prime_UTR 35.0%
CTCCCTAAAATTCACACCTA+GGG + chr7_4:3723057-3723076 Msa1135900:five_prime_UTR 40.0%
!! TGGCCAATTAGCTTTGGAAA+AGG - chr7_4:3723098-3723117 None:intergenic 40.0%
GGTATAAGGAAGGAAAAGGA+TGG - chr7_4:3723179-3723198 None:intergenic 40.0%
! TGGTGAAAGAGGTATAAGGA+AGG - chr7_4:3723189-3723208 None:intergenic 40.0%
AATGGAGAAAGTGGTGAAAG+AGG - chr7_4:3723200-3723219 None:intergenic 40.0%
ATATGTCTAGTCGAAGGTCA+AGG + chr7_4:3723410-3723429 Msa1135900:exon 40.0%
TGCAAACAAGGTCGATGATT+TGG - chr7_4:3723471-3723490 None:intergenic 40.0%
GTTGACGCAATTTGCAAACA+AGG - chr7_4:3723483-3723502 None:intergenic 40.0%
TTGTTCCTGAGATTCGTAAC+AGG + chr7_4:3723503-3723522 Msa1135900:CDS 40.0%
! GACGGAGGTAGTATCATTTT+AGG - chr7_4:3723679-3723698 None:intergenic 40.0%
TGTCAATTAAATTGGGACGG+AGG - chr7_4:3723694-3723713 None:intergenic 40.0%
GAAAATGGGTCAGTTATTCC+GGG + chr7_4:3723885-3723904 Msa1135900:intron 40.0%
TGACAAGAACCTCTAAACCA+TGG + chr7_4:3723935-3723954 Msa1135900:intron 40.0%
TAACCAATGATAGTAAGGGG+TGG + chr7_4:3724269-3724288 Msa1135900:intron 40.0%
TATCCACCCCTTACTATCAT+TGG - chr7_4:3724275-3724294 None:intergenic 40.0%
AAGGGGTGGATATCTCAATT+TGG + chr7_4:3724283-3724302 Msa1135900:intron 40.0%
!!! CACACCCACTAGTTTTTATG+TGG + chr7_4:3724553-3724572 Msa1135900:intron 40.0%
CACTCCCACATAAAAACTAG+TGG - chr7_4:3724790-3724809 None:intergenic 40.0%
! AAATCCTATAGGGGTTTGCT+AGG + chr7_4:3724677-3724696 Msa1135900:intron 40.0%
CACTCCCACATAAAAACTAG+TGG + chr7_4:3724790-3724809 Msa1135900:intron 40.0%
!!! CACACCCACTAGTTTTTATG+TGG - chr7_4:3724553-3724572 None:intergenic 40.0%
GACTATTATTGTGAGACGGA+GGG - chr7_4:3724959-3724978 None:intergenic 40.0%
AGACTATTATTGTGAGACGG+AGG - chr7_4:3724960-3724979 None:intergenic 40.0%
! TGTCGTTTAAGGTTTGTGCA+CGG - chr7_4:3725187-3725206 None:intergenic 40.0%
CGACAATAATTTAGAGACGG+AGG + chr7_4:3725202-3725221 Msa1135900:intron 40.0%
GGAGGGAGTAACACTTATTT+GGG + chr7_4:3725220-3725239 Msa1135900:intron 40.0%
! AAACTTAGGCTGAAGTTGGT+TGG + chr7_4:3725279-3725298 Msa1135900:intron 40.0%
! AACTTAGGCTGAAGTTGGTT+GGG + chr7_4:3725280-3725299 Msa1135900:intron 40.0%
GATAAAGAAGCAGCATGCAT+TGG - chr7_4:3725826-3725845 None:intergenic 40.0%
TCGAGATTCAATACATAGGG+AGG + chr7_4:3726157-3726176 Msa1135900:intron 40.0%
AGATTCAATACATAGGGAGG+AGG + chr7_4:3726160-3726179 Msa1135900:intron 40.0%
!! GGTTAATTTTGACCGACTCA+TGG + chr7_4:3726209-3726228 Msa1135900:intron 40.0%
TGCATCCACTATGTCAGTAT+CGG - chr7_4:3726381-3726400 None:intergenic 40.0%
! TCTGAGATTGTCTCAGTTGT+TGG + chr7_4:3726480-3726499 Msa1135900:CDS 40.0%
GTTAAGTAGGCTCCTAATGA+TGG - chr7_4:3726536-3726555 None:intergenic 40.0%
!! CCTAGGTGTGAATTTTAGGG+AGG - chr7_4:3723059-3723078 None:intergenic 45.0%
CCTCCCTAAAATTCACACCT+AGG + chr7_4:3723056-3723075 Msa1135900:five_prime_UTR 45.0%
! CTTTGGAAAAGGAGTGGACT+AGG - chr7_4:3723087-3723106 None:intergenic 45.0%
! TATAGTGGAAGGGGAATGGA+AGG - chr7_4:3723264-3723283 None:intergenic 45.0%
AACAATCAGGAGGGTCTACT+CGG + chr7_4:3723434-3723453 Msa1135900:CDS 45.0%
ATCAAGATAGTACTCCGTCC+CGG - chr7_4:3723906-3723925 None:intergenic 45.0%
GTATCCTAGCAAACCCCTAT+AGG - chr7_4:3724684-3724703 None:intergenic 45.0%
CGGAGGGAGTAACACTTATT+TGG + chr7_4:3725219-3725238 Msa1135900:intron 45.0%
GGACACCTCTTGAAGAAACT+AGG + chr7_4:3725333-3725352 Msa1135900:intron 45.0%
CTACGTGACGTCAATTTCGA+TGG - chr7_4:3726055-3726074 None:intergenic 45.0%
ATGCAAAGTACGGCATGTGT+AGG + chr7_4:3726352-3726371 Msa1135900:intron 45.0%
GGTAACCGATACTGACATAG+TGG + chr7_4:3726373-3726392 Msa1135900:intron 45.0%
TCTTGTAGGACCTTAGAAGC+TGG - chr7_4:3726412-3726431 None:intergenic 45.0%
GTGCTGAAGCTTCCATCATT+AGG + chr7_4:3726521-3726540 Msa1135900:CDS 45.0%
!! ATATATATATATATATATAG+GGG + chr7_4:3724520-3724539 Msa1135900:intron 5.0%
CGAAGGTCAAGGCAACAATC+AGG + chr7_4:3723421-3723440 Msa1135900:CDS 50.0%
AGGTCAAGGCAACAATCAGG+AGG + chr7_4:3723424-3723443 Msa1135900:CDS 50.0%
GGTCAAGGCAACAATCAGGA+GGG + chr7_4:3723425-3723444 Msa1135900:CDS 50.0%
ATGGGTCAGTTATTCCGGGA+CGG + chr7_4:3723889-3723908 Msa1135900:intron 50.0%
! CTGAAGTTGGTTGGGTGACT+AGG + chr7_4:3725288-3725307 Msa1135900:intron 50.0%
TGGTTGGGTGACTAGGTCAT+TGG + chr7_4:3725295-3725314 Msa1135900:intron 50.0%
!! CTGACATAGTGGATGCATGC+AGG + chr7_4:3726384-3726403 Msa1135900:intron 50.0%
CATGCAGGTACCAGCTTCTA+AGG + chr7_4:3726399-3726418 Msa1135900:intron 50.0%
!! GAGTGGACTAGGCTTTCCCT+AGG - chr7_4:3723076-3723095 None:intergenic 55.0%
GAGCGCCTGTTACGAATCTC+AGG - chr7_4:3723511-3723530 None:intergenic 55.0%
TCGTAACAGGCGCTCTGACA+AGG + chr7_4:3723516-3723535 Msa1135900:CDS 55.0%
Chromosome Type Strat End Strand Name
chr7_4 gene 3723011 3726891 3723011 ID=Msa1135900;Name=Msa1135900
chr7_4 mRNA 3723011 3726891 3723011 ID=Msa1135900-mRNA-1;Parent=Msa1135900;Name=Msa1135900-mRNA-1;_AED=0.01;_eAED=0.01;_QI=401|1|1|1|1|1|2|330|93
chr7_4 exon 3723011 3723537 3723011 ID=Msa1135900-mRNA-1:exon:1054;Parent=Msa1135900-mRNA-1
chr7_4 exon 3726406 3726891 3726406 ID=Msa1135900-mRNA-1:exon:1055;Parent=Msa1135900-mRNA-1
chr7_4 five_prime_UTR 3723011 3723411 3723011 ID=Msa1135900-mRNA-1:five_prime_utr;Parent=Msa1135900-mRNA-1
chr7_4 CDS 3723412 3723537 3723412 ID=Msa1135900-mRNA-1:cds;Parent=Msa1135900-mRNA-1
chr7_4 CDS 3726406 3726561 3726406 ID=Msa1135900-mRNA-1:cds;Parent=Msa1135900-mRNA-1
chr7_4 three_prime_UTR 3726562 3726891 3726562 ID=Msa1135900-mRNA-1:three_prime_utr;Parent=Msa1135900-mRNA-1
Gene Sequence

>Msa1135900

ATGTCTAGTCGAAGGTCAAGGCAACAATCAGGAGGGTCTACTCGGATTTCTGATGACCAAATCATCGACCTTGTTTGCAAATTGCGTCAACTTGTTCCTGAGATTCGTAACAGGCGCTCTGACAAGGTACCAGCTTCTAAGGTCCTACAAGAAACCTGCAACTATATCAGAAACTTACAAAGTGAGGTTGATGATTTAAGTCTGAGATTGTCTCAGTTGTTGGCCACAATTGATTCTGATAGTGCTGAAGCTTCCATCATTAGGAGCCTACTTAACCAATGA
Protein sequence

>Msa1135900

MSSRRSRQQSGGSTRISDDQIIDLVCKLRQLVPEIRNRRSDKVPASKVLQETCNYIRNLQSEVDDLSLRLSQLLATIDSDSAEASIIRSLLNQ*