AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1138990


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1138990 MtrunA17_Chr7g0258621 95.238 105 5 0 1 105 1 105 5.39e-58 174
Msa1138990 MtrunA17_Chr7g0258631 93.069 101 7 0 1 101 1 101 6.25e-52 158
Msa1138990 MtrunA17_Chr7g0258641 83.784 74 12 0 4 77 3 76 3.41e-36 121
Msa1138990 MtrunA17_Chr1g0175211 68.750 80 22 1 5 81 4 83 4.62e-31 108
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1138990 AT5G06760.1 65.672 67 23 0 10 76 2 68 4.86e-25 92.4

Find 42 sgRNAs with CRISPR-Local

Find 114 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAACCCTTTCTTCTTTCTTT+TGG 0.103265 7_4:-8962036 None:intergenic
GCCACCACCGGGCACCAATT+AGG 0.221115 7_4:+8962106 Msa1138990:CDS
GTTGGCAGCTGTCTCCTTAA+TGG 0.241135 7_4:-8961390 None:intergenic
CTTCTCTTGAACTGTGGCCT+TGG 0.285748 7_4:-8961447 None:intergenic
GGACACCACACCACGGGAAC+TGG 0.359178 7_4:+8962136 Msa1138990:CDS
GAAAACAGGAGAGTCCATTA+AGG 0.363155 7_4:+8961376 Msa1138990:CDS
CCACCACCGGGCACCAATTA+GGG 0.378531 7_4:+8962107 Msa1138990:CDS
GGTGTCCACCCTGCCCTAAT+TGG 0.383691 7_4:-8962120 None:intergenic
GCTCAAACGAGGAGTCAGTT+TGG 0.390088 7_4:+8962166 Msa1138990:CDS
CTCAAACGAGGAGTCAGTTT+GGG 0.391807 7_4:+8962167 Msa1138990:CDS
CAAGGAGGAGCGAAGAAAAC+AGG 0.398656 7_4:+8961362 Msa1138990:CDS
ATTGGTGCTTCAGCCAAATC+TGG 0.410427 7_4:+8961413 Msa1138990:CDS
CCCTAATTGGTGCCCGGTGG+TGG 0.412966 7_4:-8962107 None:intergenic
CACCACACCACGGGAACTGG+TGG 0.486869 7_4:+8962139 Msa1138990:CDS
CAGGGTGGACACCACACCAC+GGG 0.488696 7_4:+8962130 Msa1138990:CDS
ACCCTGCCCTAATTGGTGCC+CGG 0.493985 7_4:-8962113 None:intergenic
CACCGGGCACCAATTAGGGC+AGG 0.503630 7_4:+8962111 Msa1138990:CDS
CCTCTTTATCAAGCTCAGCC+TGG 0.513235 7_4:-8962060 None:intergenic
ATCACATGATCCCTTGCAAA+AGG 0.516543 7_4:+8962003 Msa1138990:CDS
CCAGGCTGAGCTTGATAAAG+AGG 0.523145 7_4:+8962060 Msa1138990:CDS
CCCCACCAGTTCCCGTGGTG+TGG 0.523168 7_4:-8962141 None:intergenic
TTTGGCTGAAGCACCAATGT+TGG 0.533030 7_4:-8961408 None:intergenic
AACCCAAAAGAAAGAAGAAA+GGG 0.533989 7_4:+8962033 Msa1138990:CDS
CACAACGCTGCAGCCACCAC+CGG 0.534674 7_4:+8962094 Msa1138990:CDS
AGCAGCCCCACCAGTTCCCG+TGG 0.542543 7_4:-8962146 None:intergenic
CCACACCACGGGAACTGGTG+GGG 0.542809 7_4:+8962141 Msa1138990:CDS
CAACCCAAAAGAAAGAAGAA+AGG 0.548482 7_4:+8962032 Msa1138990:CDS
GAAAGAAGAAAGGGTTAACC+AGG 0.561684 7_4:+8962042 Msa1138990:CDS
ACCACACCACGGGAACTGGT+GGG 0.566675 7_4:+8962140 Msa1138990:CDS
TGATCCCTTGCAAAAGGAGA+TGG 0.570165 7_4:+8962009 Msa1138990:CDS
AAGGAGACAGCTGCCAACAT+TGG 0.570685 7_4:+8961395 Msa1138990:CDS
ACAACGCTGCAGCCACCACC+GGG 0.572456 7_4:+8962095 Msa1138990:CDS
CAAGGCCACAGTTCAAGAGA+AGG 0.574863 7_4:+8961448 Msa1138990:CDS
TGCTTCAGCCAAATCTGGCA+TGG 0.578401 7_4:+8961418 Msa1138990:CDS
AATTACCTTCTCTTGAACTG+TGG 0.600856 7_4:-8961453 None:intergenic
GGCTGAGCTTGATAAAGAGG+CGG 0.606143 7_4:+8962063 Msa1138990:CDS
CTGGTGGGGCTGCTCAAACG+AGG 0.620605 7_4:+8962155 Msa1138990:CDS
GCAGGGTGGACACCACACCA+CGG 0.640154 7_4:+8962129 Msa1138990:CDS
ATCTGGCATGGAAAAGACCA+AGG 0.660335 7_4:+8961430 Msa1138990:CDS
ACCGGGCACCAATTAGGGCA+GGG 0.673120 7_4:+8962112 Msa1138990:CDS
CTGCCCTAATTGGTGCCCGG+TGG 0.676521 7_4:-8962110 None:intergenic
GGGCACCAATTAGGGCAGGG+TGG 0.695693 7_4:+8962115 Msa1138990:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GTTAACATATTTTTTTTTGT+TGG + chr7_4:8961671-8961690 Msa1138990:intron 15.0%
!!! GTTAACATATTTTTTTTTGT+TGG + chr7_4:8961671-8961690 Msa1138990:intron 15.0%
!! AAGGTAATTGCTTTCATTTT+TGG + chr7_4:8961467-8961486 Msa1138990:intron 25.0%
!! GTTTTGTCACTAAAATTATG+AGG - chr7_4:8961594-8961613 None:intergenic 25.0%
! GATGACAAAGTGAATTAAAA+GGG - chr7_4:8961780-8961799 None:intergenic 25.0%
! AGATGACAAAGTGAATTAAA+AGG - chr7_4:8961781-8961800 None:intergenic 25.0%
! TATATGCACATGTTTCAAAT+TGG + chr7_4:8961884-8961903 Msa1138990:intron 25.0%
!! AAGGTAATTGCTTTCATTTT+TGG + chr7_4:8961467-8961486 Msa1138990:intron 25.0%
!! GTTTTGTCACTAAAATTATG+AGG - chr7_4:8961594-8961613 None:intergenic 25.0%
! GATGACAAAGTGAATTAAAA+GGG - chr7_4:8961780-8961799 None:intergenic 25.0%
! AGATGACAAAGTGAATTAAA+AGG - chr7_4:8961781-8961800 None:intergenic 25.0%
! TATATGCACATGTTTCAAAT+TGG + chr7_4:8961884-8961903 Msa1138990:intron 25.0%
AACCCAAAAGAAAGAAGAAA+GGG + chr7_4:8962033-8962052 Msa1138990:CDS 30.0%
! AACCCTTTCTTCTTTCTTTT+GGG - chr7_4:8962038-8962057 None:intergenic 30.0%
! TAACCCTTTCTTCTTTCTTT+TGG - chr7_4:8962039-8962058 None:intergenic 30.0%
AACCCAAAAGAAAGAAGAAA+GGG + chr7_4:8962033-8962052 Msa1138990:CDS 30.0%
! AACCCTTTCTTCTTTCTTTT+GGG - chr7_4:8962038-8962057 None:intergenic 30.0%
! TAACCCTTTCTTCTTTCTTT+TGG - chr7_4:8962039-8962058 None:intergenic 30.0%
AATTACCTTCTCTTGAACTG+TGG - chr7_4:8961456-8961475 None:intergenic 35.0%
CACGAGGCTAATTATTATTG+AGG + chr7_4:8961622-8961641 Msa1138990:intron 35.0%
CAACCCAAAAGAAAGAAGAA+AGG + chr7_4:8962032-8962051 Msa1138990:CDS 35.0%
AATTACCTTCTCTTGAACTG+TGG - chr7_4:8961456-8961475 None:intergenic 35.0%
CACGAGGCTAATTATTATTG+AGG + chr7_4:8961622-8961641 Msa1138990:intron 35.0%
CAACCCAAAAGAAAGAAGAA+AGG + chr7_4:8962032-8962051 Msa1138990:CDS 35.0%
GAAAACAGGAGAGTCCATTA+AGG + chr7_4:8961376-8961395 Msa1138990:CDS 40.0%
GACAAAACTCTTTACTCACG+AGG + chr7_4:8961606-8961625 Msa1138990:intron 40.0%
TATGTTAACTAGCCACACGA+GGG - chr7_4:8961660-8961679 None:intergenic 40.0%
ATATGTTAACTAGCCACACG+AGG - chr7_4:8961661-8961680 None:intergenic 40.0%
ATCACATGATCCCTTGCAAA+AGG + chr7_4:8962003-8962022 Msa1138990:CDS 40.0%
GAAAGAAGAAAGGGTTAACC+AGG + chr7_4:8962042-8962061 Msa1138990:CDS 40.0%
GAAAACAGGAGAGTCCATTA+AGG + chr7_4:8961376-8961395 Msa1138990:CDS 40.0%
GACAAAACTCTTTACTCACG+AGG + chr7_4:8961606-8961625 Msa1138990:intron 40.0%
TATGTTAACTAGCCACACGA+GGG - chr7_4:8961660-8961679 None:intergenic 40.0%
ATATGTTAACTAGCCACACG+AGG - chr7_4:8961661-8961680 None:intergenic 40.0%
ATCACATGATCCCTTGCAAA+AGG + chr7_4:8962003-8962022 Msa1138990:CDS 40.0%
GAAAGAAGAAAGGGTTAACC+AGG + chr7_4:8962042-8962061 Msa1138990:CDS 40.0%
! TTTGGCTGAAGCACCAATGT+TGG - chr7_4:8961411-8961430 None:intergenic 45.0%
! ATTGGTGCTTCAGCCAAATC+TGG + chr7_4:8961413-8961432 Msa1138990:CDS 45.0%
! GGTCTTTTCCATGCCAGATT+TGG - chr7_4:8961429-8961448 None:intergenic 45.0%
ATCTGGCATGGAAAAGACCA+AGG + chr7_4:8961430-8961449 Msa1138990:CDS 45.0%
TGATCCCTTGCAAAAGGAGA+TGG + chr7_4:8962009-8962028 Msa1138990:CDS 45.0%
! GTTGCCATCTCCTTTTGCAA+GGG - chr7_4:8962016-8962035 None:intergenic 45.0%
CTCAAACGAGGAGTCAGTTT+GGG + chr7_4:8962167-8962186 Msa1138990:CDS 45.0%
! TTTGGCTGAAGCACCAATGT+TGG - chr7_4:8961411-8961430 None:intergenic 45.0%
! ATTGGTGCTTCAGCCAAATC+TGG + chr7_4:8961413-8961432 Msa1138990:CDS 45.0%
! GGTCTTTTCCATGCCAGATT+TGG - chr7_4:8961429-8961448 None:intergenic 45.0%
ATCTGGCATGGAAAAGACCA+AGG + chr7_4:8961430-8961449 Msa1138990:CDS 45.0%
TGATCCCTTGCAAAAGGAGA+TGG + chr7_4:8962009-8962028 Msa1138990:CDS 45.0%
! GTTGCCATCTCCTTTTGCAA+GGG - chr7_4:8962016-8962035 None:intergenic 45.0%
CTCAAACGAGGAGTCAGTTT+GGG + chr7_4:8962167-8962186 Msa1138990:CDS 45.0%
CAAGGAGGAGCGAAGAAAAC+AGG + chr7_4:8961362-8961381 Msa1138990:CDS 50.0%
GTTGGCAGCTGTCTCCTTAA+TGG - chr7_4:8961393-8961412 None:intergenic 50.0%
AAGGAGACAGCTGCCAACAT+TGG + chr7_4:8961395-8961414 Msa1138990:CDS 50.0%
TGCTTCAGCCAAATCTGGCA+TGG + chr7_4:8961418-8961437 Msa1138990:CDS 50.0%
CTTCTCTTGAACTGTGGCCT+TGG - chr7_4:8961450-8961469 None:intergenic 50.0%
CAAGGCCACAGTTCAAGAGA+AGG + chr7_4:8961448-8961467 Msa1138990:CDS 50.0%
TCAACAACTCTTCCCTCGTG+TGG + chr7_4:8961645-8961664 Msa1138990:intron 50.0%
! GGTTGCCATCTCCTTTTGCA+AGG - chr7_4:8962017-8962036 None:intergenic 50.0%
CCTCTTTATCAAGCTCAGCC+TGG - chr7_4:8962063-8962082 None:intergenic 50.0%
CCAGGCTGAGCTTGATAAAG+AGG + chr7_4:8962060-8962079 Msa1138990:CDS 50.0%
GGCTGAGCTTGATAAAGAGG+CGG + chr7_4:8962063-8962082 Msa1138990:CDS 50.0%
GCTCAAACGAGGAGTCAGTT+TGG + chr7_4:8962166-8962185 Msa1138990:CDS 50.0%
CAAGGAGGAGCGAAGAAAAC+AGG + chr7_4:8961362-8961381 Msa1138990:CDS 50.0%
GTTGGCAGCTGTCTCCTTAA+TGG - chr7_4:8961393-8961412 None:intergenic 50.0%
AAGGAGACAGCTGCCAACAT+TGG + chr7_4:8961395-8961414 Msa1138990:CDS 50.0%
TGCTTCAGCCAAATCTGGCA+TGG + chr7_4:8961418-8961437 Msa1138990:CDS 50.0%
CTTCTCTTGAACTGTGGCCT+TGG - chr7_4:8961450-8961469 None:intergenic 50.0%
CAAGGCCACAGTTCAAGAGA+AGG + chr7_4:8961448-8961467 Msa1138990:CDS 50.0%
TCAACAACTCTTCCCTCGTG+TGG + chr7_4:8961645-8961664 Msa1138990:intron 50.0%
! GGTTGCCATCTCCTTTTGCA+AGG - chr7_4:8962017-8962036 None:intergenic 50.0%
CCTCTTTATCAAGCTCAGCC+TGG - chr7_4:8962063-8962082 None:intergenic 50.0%
CCAGGCTGAGCTTGATAAAG+AGG + chr7_4:8962060-8962079 Msa1138990:CDS 50.0%
GGCTGAGCTTGATAAAGAGG+CGG + chr7_4:8962063-8962082 Msa1138990:CDS 50.0%
GCTCAAACGAGGAGTCAGTT+TGG + chr7_4:8962166-8962185 Msa1138990:CDS 50.0%
CCACCACCGGGCACCAATTA+GGG + chr7_4:8962107-8962126 Msa1138990:CDS 60.0%
ACCGGGCACCAATTAGGGCA+GGG + chr7_4:8962112-8962131 Msa1138990:CDS 60.0%
! ACCCTGCCCTAATTGGTGCC+CGG - chr7_4:8962116-8962135 None:intergenic 60.0%
GGTGTCCACCCTGCCCTAAT+TGG - chr7_4:8962123-8962142 None:intergenic 60.0%
ACCACACCACGGGAACTGGT+GGG + chr7_4:8962140-8962159 Msa1138990:CDS 60.0%
CCACCACCGGGCACCAATTA+GGG + chr7_4:8962107-8962126 Msa1138990:CDS 60.0%
ACCGGGCACCAATTAGGGCA+GGG + chr7_4:8962112-8962131 Msa1138990:CDS 60.0%
! ACCCTGCCCTAATTGGTGCC+CGG - chr7_4:8962116-8962135 None:intergenic 60.0%
GGTGTCCACCCTGCCCTAAT+TGG - chr7_4:8962123-8962142 None:intergenic 60.0%
ACCACACCACGGGAACTGGT+GGG + chr7_4:8962140-8962159 Msa1138990:CDS 60.0%
CACAACGCTGCAGCCACCAC+CGG + chr7_4:8962094-8962113 Msa1138990:CDS 65.0%
ACAACGCTGCAGCCACCACC+GGG + chr7_4:8962095-8962114 Msa1138990:CDS 65.0%
GCCACCACCGGGCACCAATT+AGG + chr7_4:8962106-8962125 Msa1138990:CDS 65.0%
! CCCTAATTGGTGCCCGGTGG+TGG - chr7_4:8962110-8962129 None:intergenic 65.0%
! CTGCCCTAATTGGTGCCCGG+TGG - chr7_4:8962113-8962132 None:intergenic 65.0%
CACCGGGCACCAATTAGGGC+AGG + chr7_4:8962111-8962130 Msa1138990:CDS 65.0%
GGGCACCAATTAGGGCAGGG+TGG + chr7_4:8962115-8962134 Msa1138990:CDS 65.0%
GCAGGGTGGACACCACACCA+CGG + chr7_4:8962129-8962148 Msa1138990:CDS 65.0%
CAGGGTGGACACCACACCAC+GGG + chr7_4:8962130-8962149 Msa1138990:CDS 65.0%
GGACACCACACCACGGGAAC+TGG + chr7_4:8962136-8962155 Msa1138990:CDS 65.0%
CACCACACCACGGGAACTGG+TGG + chr7_4:8962139-8962158 Msa1138990:CDS 65.0%
CCACACCACGGGAACTGGTG+GGG + chr7_4:8962141-8962160 Msa1138990:CDS 65.0%
CTGGTGGGGCTGCTCAAACG+AGG + chr7_4:8962155-8962174 Msa1138990:CDS 65.0%
CACAACGCTGCAGCCACCAC+CGG + chr7_4:8962094-8962113 Msa1138990:CDS 65.0%
ACAACGCTGCAGCCACCACC+GGG + chr7_4:8962095-8962114 Msa1138990:CDS 65.0%
GCCACCACCGGGCACCAATT+AGG + chr7_4:8962106-8962125 Msa1138990:CDS 65.0%
! CCCTAATTGGTGCCCGGTGG+TGG - chr7_4:8962110-8962129 None:intergenic 65.0%
! CTGCCCTAATTGGTGCCCGG+TGG - chr7_4:8962113-8962132 None:intergenic 65.0%
CACCGGGCACCAATTAGGGC+AGG + chr7_4:8962111-8962130 Msa1138990:CDS 65.0%
GGGCACCAATTAGGGCAGGG+TGG + chr7_4:8962115-8962134 Msa1138990:CDS 65.0%
GCAGGGTGGACACCACACCA+CGG + chr7_4:8962129-8962148 Msa1138990:CDS 65.0%
CAGGGTGGACACCACACCAC+GGG + chr7_4:8962130-8962149 Msa1138990:CDS 65.0%
GGACACCACACCACGGGAAC+TGG + chr7_4:8962136-8962155 Msa1138990:CDS 65.0%
CACCACACCACGGGAACTGG+TGG + chr7_4:8962139-8962158 Msa1138990:CDS 65.0%
CCACACCACGGGAACTGGTG+GGG + chr7_4:8962141-8962160 Msa1138990:CDS 65.0%
CTGGTGGGGCTGCTCAAACG+AGG + chr7_4:8962155-8962174 Msa1138990:CDS 65.0%
CCCCACCAGTTCCCGTGGTG+TGG - chr7_4:8962144-8962163 None:intergenic 70.0%
AGCAGCCCCACCAGTTCCCG+TGG - chr7_4:8962149-8962168 None:intergenic 70.0%
CCCCACCAGTTCCCGTGGTG+TGG - chr7_4:8962144-8962163 None:intergenic 70.0%
AGCAGCCCCACCAGTTCCCG+TGG - chr7_4:8962149-8962168 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr7_4 gene 8961359 8962195 8961359 ID=Msa1138990;Name=Msa1138990
chr7_4 mRNA 8961359 8962195 8961359 ID=Msa1138990-mRNA-1;Parent=Msa1138990;Name=Msa1138990-mRNA-1;_AED=0.04;_eAED=0.04;_QI=0|0|0|1|1|1|2|0|105
chr7_4 exon 8961359 8961469 8961359 ID=Msa1138990-mRNA-1:exon:2353;Parent=Msa1138990-mRNA-1
chr7_4 exon 8961989 8962195 8961989 ID=Msa1138990-mRNA-1:exon:2354;Parent=Msa1138990-mRNA-1
chr7_4 CDS 8961359 8961469 8961359 ID=Msa1138990-mRNA-1:cds;Parent=Msa1138990-mRNA-1
chr7_4 CDS 8961989 8962195 8961989 ID=Msa1138990-mRNA-1:cds;Parent=Msa1138990-mRNA-1
Gene Sequence

>Msa1138990

ATGCAAGGAGGAGCGAAGAAAACAGGAGAGTCCATTAAGGAGACAGCTGCCAACATTGGTGCTTCAGCCAAATCTGGCATGGAAAAGACCAAGGCCACAGTTCAAGAGAAGACAGAGAAGATGACATCACATGATCCCTTGCAAAAGGAGATGGCAACCCAAAAGAAAGAAGAAAGGGTTAACCAGGCTGAGCTTGATAAAGAGGCGGCGCGTGAACACAACGCTGCAGCCACCACCGGGCACCAATTAGGGCAGGGTGGACACCACACCACGGGAACTGGTGGGGCTGCTCAAACGAGGAGTCAGTTTGGGCTGTGA

Protein sequence

>Msa1138990

MQGGAKKTGESIKETAANIGASAKSGMEKTKATVQEKTEKMTSHDPLQKEMATQKKEERVNQAELDKEAAREHNAAATTGHQLGQGGHHTTGTGGAAQTRSQFGL*