AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1116140


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1116140 MtrunA17_Chr7g0258621 95.385 65 3 0 1 65 41 105 1.99e-31 105
Msa1116140 MtrunA17_Chr7g0258631 90.164 61 6 0 1 61 41 101 1.66e-24 87.4
Msa1116140 MtrunA17_Chr7g0258641 77.778 36 8 0 1 36 40 75 4.27e-13 60.1
Msa1116140 MtrunA17_Chr1g0175211 59.091 44 15 1 1 41 40 83 2.31e-11 55.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 30 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAACCCTTTCTTCTTTCTTT+TGG 0.103265 7_3:+63726881 None:intergenic
AGCCACCACCGGGCACCAAT+TGG 0.345146 7_3:-63726812 Msa1116140:CDS
GCCACCACCGGGCACCAATT+GGG 0.352332 7_3:-63726811 Msa1116140:CDS
GGACACCACACCACGGGAAC+TGG 0.359178 7_3:-63726781 Msa1116140:CDS
GCTCAAACGAGGAGTCAGTT+TGG 0.390088 7_3:-63726751 Msa1116140:CDS
CTCAAACGAGGAGTCAGTTT+GGG 0.391807 7_3:-63726750 Msa1116140:CDS
GGTGTCCACCCTGCCCCAAT+TGG 0.432127 7_3:+63726797 None:intergenic
CCCCAATTGGTGCCCGGTGG+TGG 0.440868 7_3:+63726810 None:intergenic
CACCACACCACGGGAACTGG+TGG 0.486869 7_3:-63726778 Msa1116140:CDS
CAGGGTGGACACCACACCAC+GGG 0.488696 7_3:-63726787 Msa1116140:CDS
CCTCTTTATCAAGCTCAGCC+TGG 0.513235 7_3:+63726857 None:intergenic
ATCACATGATCCCTTGCAAA+AGG 0.516543 7_3:-63726914 Msa1116140:CDS
CCAGGCTGAGCTTGATAAAG+AGG 0.523145 7_3:-63726857 Msa1116140:CDS
CCCCACCAGTTCCCGTGGTG+TGG 0.523168 7_3:+63726776 None:intergenic
AACCCAAAAGAAAGAAGAAA+GGG 0.533989 7_3:-63726884 Msa1116140:CDS
CACAACGCTGCAGCCACCAC+CGG 0.534674 7_3:-63726823 Msa1116140:CDS
AGCAGCCCCACCAGTTCCCG+TGG 0.542543 7_3:+63726771 None:intergenic
CCACACCACGGGAACTGGTG+GGG 0.542809 7_3:-63726776 Msa1116140:CDS
CAACCCAAAAGAAAGAAGAA+AGG 0.548482 7_3:-63726885 Msa1116140:CDS
CCACCACCGGGCACCAATTG+GGG 0.551603 7_3:-63726810 Msa1116140:CDS
GAAAGAAGAAAGGGTTAACC+AGG 0.561684 7_3:-63726875 Msa1116140:CDS
ACCACACCACGGGAACTGGT+GGG 0.566675 7_3:-63726777 Msa1116140:CDS
TGATCCCTTGCAAAAGGAGA+TGG 0.570165 7_3:-63726908 Msa1116140:CDS
ACAACGCTGCAGCCACCACC+GGG 0.572456 7_3:-63726822 Msa1116140:CDS
GGCTGAGCTTGATAAAGAGG+CGG 0.606143 7_3:-63726854 Msa1116140:CDS
CTGGTGGGGCTGCTCAAACG+AGG 0.620605 7_3:-63726762 Msa1116140:CDS
GCAGGGTGGACACCACACCA+CGG 0.630908 7_3:-63726788 Msa1116140:CDS
ACCGGGCACCAATTGGGGCA+GGG 0.632230 7_3:-63726805 Msa1116140:CDS
GGGCACCAATTGGGGCAGGG+TGG 0.662871 7_3:-63726802 Msa1116140:CDS
CTGCCCCAATTGGTGCCCGG+TGG 0.696266 7_3:+63726807 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AACCCAAAAGAAAGAAGAAA+GGG - chr7_3:63726779-63726798 Msa1116140:CDS 30.0%
! AACCCTTTCTTCTTTCTTTT+GGG + chr7_3:63726784-63726803 None:intergenic 30.0%
! TAACCCTTTCTTCTTTCTTT+TGG + chr7_3:63726785-63726804 None:intergenic 30.0%
CAACCCAAAAGAAAGAAGAA+AGG - chr7_3:63726778-63726797 Msa1116140:CDS 35.0%
ATCACATGATCCCTTGCAAA+AGG - chr7_3:63726749-63726768 Msa1116140:CDS 40.0%
GAAAGAAGAAAGGGTTAACC+AGG - chr7_3:63726788-63726807 Msa1116140:CDS 40.0%
TGATCCCTTGCAAAAGGAGA+TGG - chr7_3:63726755-63726774 Msa1116140:CDS 45.0%
! GTTGCCATCTCCTTTTGCAA+GGG + chr7_3:63726762-63726781 None:intergenic 45.0%
CTCAAACGAGGAGTCAGTTT+GGG - chr7_3:63726913-63726932 Msa1116140:CDS 45.0%
! GGTTGCCATCTCCTTTTGCA+AGG + chr7_3:63726763-63726782 None:intergenic 50.0%
CCTCTTTATCAAGCTCAGCC+TGG + chr7_3:63726809-63726828 None:intergenic 50.0%
CCAGGCTGAGCTTGATAAAG+AGG - chr7_3:63726806-63726825 Msa1116140:CDS 50.0%
GGCTGAGCTTGATAAAGAGG+CGG - chr7_3:63726809-63726828 Msa1116140:CDS 50.0%
GCTCAAACGAGGAGTCAGTT+TGG - chr7_3:63726912-63726931 Msa1116140:CDS 50.0%
ACCACACCACGGGAACTGGT+GGG - chr7_3:63726886-63726905 Msa1116140:CDS 60.0%
CACAACGCTGCAGCCACCAC+CGG - chr7_3:63726840-63726859 Msa1116140:CDS 65.0%
ACAACGCTGCAGCCACCACC+GGG - chr7_3:63726841-63726860 Msa1116140:CDS 65.0%
AGCCACCACCGGGCACCAAT+TGG - chr7_3:63726851-63726870 Msa1116140:CDS 65.0%
GCCACCACCGGGCACCAATT+GGG - chr7_3:63726852-63726871 Msa1116140:CDS 65.0%
CCACCACCGGGCACCAATTG+GGG - chr7_3:63726853-63726872 Msa1116140:CDS 65.0%
ACCGGGCACCAATTGGGGCA+GGG - chr7_3:63726858-63726877 Msa1116140:CDS 65.0%
! ACCCTGCCCCAATTGGTGCC+CGG + chr7_3:63726862-63726881 None:intergenic 65.0%
GGTGTCCACCCTGCCCCAAT+TGG + chr7_3:63726869-63726888 None:intergenic 65.0%
GCAGGGTGGACACCACACCA+CGG - chr7_3:63726875-63726894 Msa1116140:CDS 65.0%
CAGGGTGGACACCACACCAC+GGG - chr7_3:63726876-63726895 Msa1116140:CDS 65.0%
GGACACCACACCACGGGAAC+TGG - chr7_3:63726882-63726901 Msa1116140:CDS 65.0%
CACCACACCACGGGAACTGG+TGG - chr7_3:63726885-63726904 Msa1116140:CDS 65.0%
CCACACCACGGGAACTGGTG+GGG - chr7_3:63726887-63726906 Msa1116140:CDS 65.0%
CTGGTGGGGCTGCTCAAACG+AGG - chr7_3:63726901-63726920 Msa1116140:CDS 65.0%
! CCCCAATTGGTGCCCGGTGG+TGG + chr7_3:63726856-63726875 None:intergenic 70.0%
! CTGCCCCAATTGGTGCCCGG+TGG + chr7_3:63726859-63726878 None:intergenic 70.0%
CACCGGGCACCAATTGGGGC+AGG - chr7_3:63726857-63726876 Msa1116140:CDS 70.0%
GGGCACCAATTGGGGCAGGG+TGG - chr7_3:63726861-63726880 Msa1116140:CDS 70.0%
CCCCACCAGTTCCCGTGGTG+TGG + chr7_3:63726890-63726909 None:intergenic 70.0%
AGCAGCCCCACCAGTTCCCG+TGG + chr7_3:63726895-63726914 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr7_3 gene 63726744 63726941 63726744 ID=Msa1116140;Name=Msa1116140
chr7_3 mRNA 63726744 63726941 63726744 ID=Msa1116140-mRNA-1;Parent=Msa1116140;Name=Msa1116140-mRNA-1;_AED=0.44;_eAED=0.44;_QI=0|-1|0|1|-1|1|1|0|65
chr7_3 exon 63726744 63726941 63726744 ID=Msa1116140-mRNA-1:exon:15014;Parent=Msa1116140-mRNA-1
chr7_3 CDS 63726744 63726941 63726744 ID=Msa1116140-mRNA-1:cds;Parent=Msa1116140-mRNA-1
Gene Sequence

>Msa1116140

ATGACATCACATGATCCCTTGCAAAAGGAGATGGCAACCCAAAAGAAAGAAGAAAGGGTTAACCAGGCTGAGCTTGATAAAGAGGCGGCGCGTGAACACAACGCTGCAGCCACCACCGGGCACCAATTGGGGCAGGGTGGACACCACACCACGGGAACTGGTGGGGCTGCTCAAACGAGGAGTCAGTTTGGGCTGTGA

Protein sequence

>Msa1116140

MTSHDPLQKEMATQKKEERVNQAELDKEAAREHNAAATTGHQLGQGGHHTTGTGGAAQTRSQFGL*