AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1139880

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1139880 MtrunA17_Chr7g0220931 93.421 380 17 4 1 378 1 374 0.0 542
Msa1139880 MtrunA17_Chr6g0456881 46.328 354 109 16 73 364 67 401 5.46e-67 218
Msa1139880 MtrunA17_Chr1g0205131 35.340 382 164 14 43 356 27 393 7.12e-43 154
Msa1139880 MtrunA17_Chr4g0062691 33.459 266 141 11 5 245 14 268 7.53e-27 110
Msa1139880 MtrunA17_Chr8g0376571 52.703 74 35 0 116 189 57 130 1.22e-18 87.4
Msa1139880 MtrunA17_Chr2g0316391 53.846 65 30 0 115 179 15 79 5.02e-17 80.9
Msa1139880 MtrunA17_Chr2g0325171 51.562 64 31 0 115 178 19 82 1.29e-16 80.1
Msa1139880 MtrunA17_Chr8g0360441 52.308 65 31 0 115 179 49 113 1.53e-16 80.9
Msa1139880 MtrunA17_Chr4g0040921 52.308 65 31 0 115 179 17 81 1.65e-16 79.7
Msa1139880 MtrunA17_Chr6g0454781 47.561 82 41 1 119 200 48 127 3.82e-16 78.6
Msa1139880 MtrunA17_Chr7g0219461 51.667 60 29 0 119 178 56 115 1.56e-14 73.9
Msa1139880 MtrunA17_Chr4g0061561 48.333 60 31 0 119 178 56 115 4.55e-13 69.3
Msa1139880 MtrunA17_Chr3g0086961 48.333 60 31 0 119 178 56 115 2.30e-12 67.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1139880 AT1G67260.2 37.546 269 129 12 73 312 47 305 5.80e-34 129
Msa1139880 AT1G67260.3 37.546 269 129 12 73 312 47 305 5.80e-34 129
Msa1139880 AT1G67260.1 37.546 269 129 12 73 312 47 305 6.34e-34 129
Msa1139880 AT1G68800.1 54.867 113 42 4 120 229 112 218 2.19e-25 105
Msa1139880 AT1G68800.2 54.867 113 42 4 120 229 112 218 2.36e-25 105
Msa1139880 AT3G18550.2 39.216 153 69 3 113 241 141 293 8.88e-22 96.7
Msa1139880 AT3G18550.3 36.943 157 71 3 113 241 141 297 1.30e-20 93.2
Msa1139880 AT3G18550.1 36.943 157 71 3 113 241 141 297 1.88e-20 92.8
Msa1139880 AT3G18550.4 36.943 157 71 3 113 241 141 297 1.88e-20 92.8
Msa1139880 AT4G18390.1 37.671 146 87 3 103 248 27 168 2.59e-18 85.9
Msa1139880 AT4G18390.2 37.671 146 87 3 103 248 27 168 2.59e-18 85.9
Msa1139880 AT1G30210.2 53.968 63 29 0 116 178 46 108 1.73e-16 80.1
Msa1139880 AT1G30210.1 53.968 63 29 0 116 178 46 108 1.73e-16 80.1
Msa1139880 AT1G53230.1 51.515 66 32 0 115 180 44 109 2.52e-16 80.1
Msa1139880 AT1G53230.2 51.515 66 32 0 115 180 44 109 2.52e-16 80.1
Msa1139880 AT3G15030.2 52.308 65 31 0 115 179 40 104 2.97e-16 80.1
Msa1139880 AT3G15030.4 52.308 65 31 0 115 179 40 104 2.97e-16 80.1
Msa1139880 AT3G15030.3 52.308 65 31 0 115 179 40 104 2.97e-16 80.1
Msa1139880 AT3G15030.1 52.308 65 31 0 115 179 40 104 2.97e-16 80.1
Msa1139880 AT2G31070.1 51.562 64 31 0 115 178 24 87 6.03e-16 79.0
Msa1139880 AT3G02150.1 53.333 60 28 0 119 178 73 132 1.11e-14 73.9
Msa1139880 AT3G02150.2 53.333 60 28 0 119 178 73 132 1.84e-14 74.3
Msa1139880 AT5G60970.1 46.667 60 32 0 119 178 62 121 9.30e-13 69.3
Msa1139880 AT5G08070.2 45.000 60 33 0 119 178 32 91 2.37e-11 63.9
Msa1139880 AT5G08070.1 45.000 60 33 0 119 178 32 91 2.37e-11 63.9

Find 83 sgRNAs with CRISPR-Local

Find 192 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACACGGATATTAAATATTTA+AGG 0.113050 7_4:-10695861 None:intergenic
CCTTCAACTTATTGCTCTTT+AGG 0.129653 7_4:+10696020 Msa1139880:CDS
ATCCTGATACACTAACAAAT+TGG 0.193845 7_4:+10696246 Msa1139880:CDS
GCCCAATTTGTTAGTGTATC+AGG 0.208804 7_4:-10696248 None:intergenic
CTCCAATGTTATCACCAAAT+TGG 0.217754 7_4:+10696975 Msa1139880:CDS
GTTAGTGTATCAGGATTAAT+TGG 0.223236 7_4:-10696239 None:intergenic
GGTATATAGCTATGTTCTTA+TGG 0.234961 7_4:-10695919 None:intergenic
CTTCGAAGACGAAGGATTCT+AGG 0.286350 7_4:+10696681 Msa1139880:CDS
TTCTTAGCTGGTTTCTTGAC+AGG 0.328037 7_4:-10696353 None:intergenic
TGTGAAGAAGAAGATGAATT+AGG 0.330614 7_4:-10696062 None:intergenic
CACGGATATTAAATATTTAA+GGG 0.331420 7_4:-10695860 None:intergenic
TTCGATCTTCAAGACATGTT+AGG 0.334610 7_4:+10696458 Msa1139880:CDS
CTATGCCTGTCTTTCTTAGC+TGG 0.335007 7_4:-10696365 None:intergenic
TTTGAAGTTTCATCCTCATT+AGG 0.336904 7_4:-10696818 None:intergenic
TATTTAATATCCGTGTAAAA+AGG 0.339349 7_4:+10695868 Msa1139880:five_prime_UTR
TTATTATTAGCATCCCAATT+TGG 0.341400 7_4:-10696989 None:intergenic
TCATGAGAATCACTATTATT+AGG 0.353829 7_4:-10696950 None:intergenic
GAGCCATTCAAGTGTGTTGC+TGG 0.358400 7_4:-10696492 None:intergenic
AATATCAGCTAATATCGATT+TGG 0.375751 7_4:-10697258 None:intergenic
ATGATATAGAAAGTGAGATT+TGG 0.376380 7_4:-10695734 None:intergenic
TCAGGGGTAAGAAAAGTAAA+AGG 0.386359 7_4:-10696095 None:intergenic
GTATATAGCTATGTTCTTAT+GGG 0.394932 7_4:-10695918 None:intergenic
TTCAAATCTTTGGAAAATCA+TGG 0.409744 7_4:+10697217 Msa1139880:CDS
AAAGTTGTTGATGTTGTTGT+TGG 0.410045 7_4:-10696159 None:intergenic
TCCAATGTTATCACCAAATT+GGG 0.411724 7_4:+10696976 Msa1139880:CDS
GATTGATTACTTAACTCAAA+TGG 0.413424 7_4:-10695986 None:intergenic
CTAAAGATAGAAAGATGAAA+AGG 0.414926 7_4:+10696645 Msa1139880:CDS
AATGAACTACTTACTAGTTA+AGG 0.419311 7_4:-10695836 None:intergenic
TCTTGATATTGCAGAAAAGT+TGG 0.424180 7_4:-10697038 None:intergenic
TCTTCTTCGTCTTCCGATGA+AGG 0.430527 7_4:+10696605 Msa1139880:CDS
TCCTGATACACTAACAAATT+GGG 0.434497 7_4:+10696247 Msa1139880:CDS
TCGATCTTCAAGACATGTTA+GGG 0.442491 7_4:+10696459 Msa1139880:CDS
GGTGTTGTTAGTGTTGTTGT+TGG 0.445348 7_4:-10696138 None:intergenic
AGCAAGAACAACATAGCTTC+TGG 0.450038 7_4:+10696554 Msa1139880:CDS
ATCAATTGATGAAGGATTTG+TGG 0.452195 7_4:-10696794 None:intergenic
TTGTTGTGAAACAAGAAACT+AGG 0.471445 7_4:+10695955 Msa1139880:five_prime_UTR
TGAAAGAAATTGATGATGAT+AGG 0.471978 7_4:-10695779 None:intergenic
TGATGAGTTGTTTGTGTGTA+GGG 0.490224 7_4:-10696209 None:intergenic
AATAACAATGATGCTGTCAC+TGG 0.496363 7_4:+10697010 Msa1139880:CDS
CATTAGGCATCAATTGATGA+AGG 0.499616 7_4:-10696802 None:intergenic
CCTAAAGAGCAATAAGTTGA+AGG 0.500471 7_4:-10696020 None:intergenic
TCAAGAACGAATCTATCATC+AGG 0.510472 7_4:+10697055 Msa1139880:CDS
ATCTTCTTCATCTCCTTCAT+CGG 0.512276 7_4:-10696618 None:intergenic
AAAGATGAAAAGGGCACAGA+AGG 0.517893 7_4:+10696655 Msa1139880:CDS
AGCAAGATTCACACATCTCA+AGG 0.519514 7_4:+10696386 Msa1139880:CDS
TGTTGTGAAACAAGAAACTA+GGG 0.521317 7_4:+10695956 Msa1139880:five_prime_UTR
TAAATCAAAAGAAGCAATTG+AGG 0.525585 7_4:+10696520 Msa1139880:CDS
TAAAGATAGAAAGATGAAAA+GGG 0.526209 7_4:+10696646 Msa1139880:CDS
AAGGCTTATTCTTCTAACAA+TGG 0.526389 7_4:+10695699 Msa1139880:five_prime_UTR
TCAAATCTTTGGAAAATCAT+GGG 0.526493 7_4:+10697218 Msa1139880:CDS
GAACTTCCTCGCGATCTCGA+TGG 0.528472 7_4:-10696435 None:intergenic
TAGGGAACAGAAATAGCTGC+TGG 0.529350 7_4:-10696191 None:intergenic
AGAAAACAAGAGCAAGAGCA+AGG 0.530089 7_4:+10696705 Msa1139880:CDS
AAGCCAGCAACACACTTGAA+TGG 0.532128 7_4:+10696489 Msa1139880:CDS
AGAAACCAGCTAAGAAAGAC+AGG 0.535037 7_4:+10696360 Msa1139880:CDS
AACCTAGAAGAGAATAAAAG+TGG 0.536483 7_4:-10695804 None:intergenic
TCATCAATTGATGCCTAATG+AGG 0.536962 7_4:+10696805 Msa1139880:CDS
TTCGAAGACGAAGGATTCTA+GGG 0.537014 7_4:+10696682 Msa1139880:CDS
TGTGTGATGGACAAGGCTCA+TGG 0.546089 7_4:-10695664 None:intergenic
TGATATCTTTAACTTCATTG+AGG 0.548363 7_4:+10696856 Msa1139880:CDS
GGGGTAAGAAAAGTAAAAGG+AGG 0.548490 7_4:-10696092 None:intergenic
TCATATTTATCTGTGTGTGA+TGG 0.554232 7_4:-10695677 None:intergenic
TTAGGATTATAACAAGGGTA+AGG 0.556043 7_4:-10696044 None:intergenic
TCCCAATTTGGTGATAACAT+TGG 0.563066 7_4:-10696977 None:intergenic
TCACACACAGATAAATATGA+AGG 0.567557 7_4:+10695680 Msa1139880:five_prime_UTR
ATGAATTAGGATTATAACAA+GGG 0.570279 7_4:-10696049 None:intergenic
GGGTAAGAAAAGTAAAAGGA+GGG 0.575339 7_4:-10696091 None:intergenic
GTGTGATGGACAAGGCTCAT+GGG 0.578531 7_4:-10695663 None:intergenic
ATGATGAGTTGTTTGTGTGT+AGG 0.581066 7_4:-10696210 None:intergenic
AATTGATGATGATAGGTGAA+GGG 0.587918 7_4:-10695772 None:intergenic
CAGCTAATATCGATTTGGAC+TGG 0.588709 7_4:-10697253 None:intergenic
GAAAACAAGAGCAAGAGCAA+GGG 0.591086 7_4:+10696706 Msa1139880:CDS
GATGAATTAGGATTATAACA+AGG 0.592158 7_4:-10696050 None:intergenic
CTCAAGGTTTGAGAGACAGA+AGG 0.592962 7_4:+10696402 Msa1139880:CDS
AAATTGATGATGATAGGTGA+AGG 0.598522 7_4:-10695773 None:intergenic
GACTTTCCATCGAGATCGCG+AGG 0.608900 7_4:+10696429 Msa1139880:CDS
GATGGACAAGGCTCATGGGT+TGG 0.611574 7_4:-10695659 None:intergenic
TCAAGGTTTGAGAGACAGAA+GGG 0.615028 7_4:+10696403 Msa1139880:CDS
AATCTTTGGAAAATCATGGG+AGG 0.624471 7_4:+10697221 Msa1139880:CDS
GAAGGAATCTTCGAAGACGA+AGG 0.639563 7_4:+10696673 Msa1139880:CDS
GCTTCTGGCGATGACGACGA+TGG 0.659720 7_4:+10696569 Msa1139880:CDS
TTATCTGTGTGTGATGGACA+AGG 0.672150 7_4:-10695671 None:intergenic
ATGTTGAAACAAGATCTAAG+TGG 0.698091 7_4:+10696290 Msa1139880:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CATAAAATTAATTAACAAAA+AGG + chr7_4:10697120-10697139 Msa1139880:intron 10.0%
!! CATAAAATTAATTAACAAAA+AGG + chr7_4:10697120-10697139 Msa1139880:intron 10.0%
!!! TTTTTAGGTTTAAACTTTTT+AGG + chr7_4:10697192-10697211 Msa1139880:intron 15.0%
!!! TTTTAGGTTTAAACTTTTTA+GGG + chr7_4:10697193-10697212 Msa1139880:intron 15.0%
!!! TTTTTAGGTTTAAACTTTTT+AGG + chr7_4:10697192-10697211 Msa1139880:intron 15.0%
!!! TTTTAGGTTTAAACTTTTTA+GGG + chr7_4:10697193-10697212 Msa1139880:intron 15.0%
!! CACGGATATTAAATATTTAA+GGG - chr7_4:10695863-10695882 None:intergenic 20.0%
!! ACACGGATATTAAATATTTA+AGG - chr7_4:10695864-10695883 None:intergenic 20.0%
!! TATTTAATATCCGTGTAAAA+AGG + chr7_4:10695868-10695887 Msa1139880:five_prime_UTR 20.0%
!! ATGAATTAGGATTATAACAA+GGG - chr7_4:10696052-10696071 None:intergenic 20.0%
!! TAAAGATAGAAAGATGAAAA+GGG + chr7_4:10696646-10696665 Msa1139880:CDS 20.0%
!!! TTGTGATACTTACAATTTTT+AGG + chr7_4:10697177-10697196 Msa1139880:intron 20.0%
!! CACGGATATTAAATATTTAA+GGG - chr7_4:10695863-10695882 None:intergenic 20.0%
!! ACACGGATATTAAATATTTA+AGG - chr7_4:10695864-10695883 None:intergenic 20.0%
!! TATTTAATATCCGTGTAAAA+AGG + chr7_4:10695868-10695887 Msa1139880:five_prime_UTR 20.0%
!! ATGAATTAGGATTATAACAA+GGG - chr7_4:10696052-10696071 None:intergenic 20.0%
!! TAAAGATAGAAAGATGAAAA+GGG + chr7_4:10696646-10696665 Msa1139880:CDS 20.0%
!!! TTGTGATACTTACAATTTTT+AGG + chr7_4:10697177-10697196 Msa1139880:intron 20.0%
! ATGATATAGAAAGTGAGATT+TGG - chr7_4:10695737-10695756 None:intergenic 25.0%
!!! TGAAAGAAATTGATGATGAT+AGG - chr7_4:10695782-10695801 None:intergenic 25.0%
! AATGAACTACTTACTAGTTA+AGG - chr7_4:10695839-10695858 None:intergenic 25.0%
! GTATATAGCTATGTTCTTAT+GGG - chr7_4:10695921-10695940 None:intergenic 25.0%
! GATTGATTACTTAACTCAAA+TGG - chr7_4:10695989-10696008 None:intergenic 25.0%
! GATGAATTAGGATTATAACA+AGG - chr7_4:10696053-10696072 None:intergenic 25.0%
! TAAATCAAAAGAAGCAATTG+AGG + chr7_4:10696520-10696539 Msa1139880:CDS 25.0%
! CTAAAGATAGAAAGATGAAA+AGG + chr7_4:10696645-10696664 Msa1139880:CDS 25.0%
!! TGATATCTTTAACTTCATTG+AGG + chr7_4:10696856-10696875 Msa1139880:CDS 25.0%
! TCATGAGAATCACTATTATT+AGG - chr7_4:10696953-10696972 None:intergenic 25.0%
! TTATTATTAGCATCCCAATT+TGG - chr7_4:10696992-10697011 None:intergenic 25.0%
!! TTCAAATCTTTGGAAAATCA+TGG + chr7_4:10697217-10697236 Msa1139880:CDS 25.0%
!! TCAAATCTTTGGAAAATCAT+GGG + chr7_4:10697218-10697237 Msa1139880:CDS 25.0%
! ATGATATAGAAAGTGAGATT+TGG - chr7_4:10695737-10695756 None:intergenic 25.0%
!!! TGAAAGAAATTGATGATGAT+AGG - chr7_4:10695782-10695801 None:intergenic 25.0%
! AATGAACTACTTACTAGTTA+AGG - chr7_4:10695839-10695858 None:intergenic 25.0%
! GTATATAGCTATGTTCTTAT+GGG - chr7_4:10695921-10695940 None:intergenic 25.0%
! GATTGATTACTTAACTCAAA+TGG - chr7_4:10695989-10696008 None:intergenic 25.0%
! GATGAATTAGGATTATAACA+AGG - chr7_4:10696053-10696072 None:intergenic 25.0%
! TAAATCAAAAGAAGCAATTG+AGG + chr7_4:10696520-10696539 Msa1139880:CDS 25.0%
! CTAAAGATAGAAAGATGAAA+AGG + chr7_4:10696645-10696664 Msa1139880:CDS 25.0%
!! TGATATCTTTAACTTCATTG+AGG + chr7_4:10696856-10696875 Msa1139880:CDS 25.0%
! TCATGAGAATCACTATTATT+AGG - chr7_4:10696953-10696972 None:intergenic 25.0%
! TTATTATTAGCATCCCAATT+TGG - chr7_4:10696992-10697011 None:intergenic 25.0%
!! TTCAAATCTTTGGAAAATCA+TGG + chr7_4:10697217-10697236 Msa1139880:CDS 25.0%
!! TCAAATCTTTGGAAAATCAT+GGG + chr7_4:10697218-10697237 Msa1139880:CDS 25.0%
TCATATTTATCTGTGTGTGA+TGG - chr7_4:10695680-10695699 None:intergenic 30.0%
TCACACACAGATAAATATGA+AGG + chr7_4:10695680-10695699 Msa1139880:five_prime_UTR 30.0%
AAGGCTTATTCTTCTAACAA+TGG + chr7_4:10695699-10695718 Msa1139880:five_prime_UTR 30.0%
!! AATTGATGATGATAGGTGAA+GGG - chr7_4:10695775-10695794 None:intergenic 30.0%
!! AAATTGATGATGATAGGTGA+AGG - chr7_4:10695776-10695795 None:intergenic 30.0%
!! AACCACTTTTATTCTCTTCT+AGG + chr7_4:10695802-10695821 Msa1139880:five_prime_UTR 30.0%
AACCTAGAAGAGAATAAAAG+TGG - chr7_4:10695807-10695826 None:intergenic 30.0%
! CCTAAAAACACCTTTTTACA+CGG - chr7_4:10695881-10695900 None:intergenic 30.0%
GGTATATAGCTATGTTCTTA+TGG - chr7_4:10695922-10695941 None:intergenic 30.0%
CACAACAAAAAAAGAACAAG+AGG - chr7_4:10695943-10695962 None:intergenic 30.0%
TTGTTGTGAAACAAGAAACT+AGG + chr7_4:10695955-10695974 Msa1139880:five_prime_UTR 30.0%
TGTTGTGAAACAAGAAACTA+GGG + chr7_4:10695956-10695975 Msa1139880:five_prime_UTR 30.0%
TTAGGATTATAACAAGGGTA+AGG - chr7_4:10696047-10696066 None:intergenic 30.0%
TGTGAAGAAGAAGATGAATT+AGG - chr7_4:10696065-10696084 None:intergenic 30.0%
!!! TAGAAGAAGCATTTTGATCA+GGG - chr7_4:10696115-10696134 None:intergenic 30.0%
!!! TTAGAAGAAGCATTTTGATC+AGG - chr7_4:10696116-10696135 None:intergenic 30.0%
AAAGTTGTTGATGTTGTTGT+TGG - chr7_4:10696162-10696181 None:intergenic 30.0%
GTTAGTGTATCAGGATTAAT+TGG - chr7_4:10696242-10696261 None:intergenic 30.0%
ATCCTGATACACTAACAAAT+TGG + chr7_4:10696246-10696265 Msa1139880:CDS 30.0%
TCCTGATACACTAACAAATT+GGG + chr7_4:10696247-10696266 Msa1139880:CDS 30.0%
ATGTTGAAACAAGATCTAAG+TGG + chr7_4:10696290-10696309 Msa1139880:CDS 30.0%
!! TGTGGATTTTGATCAATTTC+AGG - chr7_4:10696779-10696798 None:intergenic 30.0%
ATCAATTGATGAAGGATTTG+TGG - chr7_4:10696797-10696816 None:intergenic 30.0%
! TTTGAAGTTTCATCCTCATT+AGG - chr7_4:10696821-10696840 None:intergenic 30.0%
! GCATCAAATTAGTGTTTTGT+TGG - chr7_4:10696931-10696950 None:intergenic 30.0%
TCCAATGTTATCACCAAATT+GGG + chr7_4:10696976-10696995 Msa1139880:CDS 30.0%
TCTTGATATTGCAGAAAAGT+TGG - chr7_4:10697041-10697060 None:intergenic 30.0%
!!! TTTTTAGGGCTTCAAATCTT+TGG + chr7_4:10697207-10697226 Msa1139880:CDS 30.0%
TCATATTTATCTGTGTGTGA+TGG - chr7_4:10695680-10695699 None:intergenic 30.0%
TCACACACAGATAAATATGA+AGG + chr7_4:10695680-10695699 Msa1139880:five_prime_UTR 30.0%
AAGGCTTATTCTTCTAACAA+TGG + chr7_4:10695699-10695718 Msa1139880:five_prime_UTR 30.0%
!! AATTGATGATGATAGGTGAA+GGG - chr7_4:10695775-10695794 None:intergenic 30.0%
!! AAATTGATGATGATAGGTGA+AGG - chr7_4:10695776-10695795 None:intergenic 30.0%
!! AACCACTTTTATTCTCTTCT+AGG + chr7_4:10695802-10695821 Msa1139880:five_prime_UTR 30.0%
AACCTAGAAGAGAATAAAAG+TGG - chr7_4:10695807-10695826 None:intergenic 30.0%
! CCTAAAAACACCTTTTTACA+CGG - chr7_4:10695881-10695900 None:intergenic 30.0%
GGTATATAGCTATGTTCTTA+TGG - chr7_4:10695922-10695941 None:intergenic 30.0%
CACAACAAAAAAAGAACAAG+AGG - chr7_4:10695943-10695962 None:intergenic 30.0%
TTGTTGTGAAACAAGAAACT+AGG + chr7_4:10695955-10695974 Msa1139880:five_prime_UTR 30.0%
TGTTGTGAAACAAGAAACTA+GGG + chr7_4:10695956-10695975 Msa1139880:five_prime_UTR 30.0%
TTAGGATTATAACAAGGGTA+AGG - chr7_4:10696047-10696066 None:intergenic 30.0%
TGTGAAGAAGAAGATGAATT+AGG - chr7_4:10696065-10696084 None:intergenic 30.0%
!!! TAGAAGAAGCATTTTGATCA+GGG - chr7_4:10696115-10696134 None:intergenic 30.0%
!!! TTAGAAGAAGCATTTTGATC+AGG - chr7_4:10696116-10696135 None:intergenic 30.0%
AAAGTTGTTGATGTTGTTGT+TGG - chr7_4:10696162-10696181 None:intergenic 30.0%
GTTAGTGTATCAGGATTAAT+TGG - chr7_4:10696242-10696261 None:intergenic 30.0%
ATCCTGATACACTAACAAAT+TGG + chr7_4:10696246-10696265 Msa1139880:CDS 30.0%
TCCTGATACACTAACAAATT+GGG + chr7_4:10696247-10696266 Msa1139880:CDS 30.0%
ATGTTGAAACAAGATCTAAG+TGG + chr7_4:10696290-10696309 Msa1139880:CDS 30.0%
!! TGTGGATTTTGATCAATTTC+AGG - chr7_4:10696779-10696798 None:intergenic 30.0%
ATCAATTGATGAAGGATTTG+TGG - chr7_4:10696797-10696816 None:intergenic 30.0%
! TTTGAAGTTTCATCCTCATT+AGG - chr7_4:10696821-10696840 None:intergenic 30.0%
! GCATCAAATTAGTGTTTTGT+TGG - chr7_4:10696931-10696950 None:intergenic 30.0%
TCCAATGTTATCACCAAATT+GGG + chr7_4:10696976-10696995 Msa1139880:CDS 30.0%
TCTTGATATTGCAGAAAAGT+TGG - chr7_4:10697041-10697060 None:intergenic 30.0%
!!! TTTTTAGGGCTTCAAATCTT+TGG + chr7_4:10697207-10697226 Msa1139880:CDS 30.0%
! TAGGTGAAGGGTTTTCATTT+TGG - chr7_4:10695763-10695782 None:intergenic 35.0%
!!! CCGTGTAAAAAGGTGTTTTT+AGG + chr7_4:10695878-10695897 Msa1139880:five_prime_UTR 35.0%
! CCTAAAGAGCAATAAGTTGA+AGG - chr7_4:10696023-10696042 None:intergenic 35.0%
CCTTCAACTTATTGCTCTTT+AGG + chr7_4:10696020-10696039 Msa1139880:CDS 35.0%
GGGTAAGAAAAGTAAAAGGA+GGG - chr7_4:10696094-10696113 None:intergenic 35.0%
TCAGGGGTAAGAAAAGTAAA+AGG - chr7_4:10696098-10696117 None:intergenic 35.0%
!!! AGAAGAAGCATTTTGATCAG+GGG - chr7_4:10696114-10696133 None:intergenic 35.0%
TGATGAGTTGTTTGTGTGTA+GGG - chr7_4:10696212-10696231 None:intergenic 35.0%
ATGATGAGTTGTTTGTGTGT+AGG - chr7_4:10696213-10696232 None:intergenic 35.0%
TTCGATCTTCAAGACATGTT+AGG + chr7_4:10696458-10696477 Msa1139880:CDS 35.0%
TCGATCTTCAAGACATGTTA+GGG + chr7_4:10696459-10696478 Msa1139880:CDS 35.0%
ATCTTCTTCATCTCCTTCAT+CGG - chr7_4:10696621-10696640 None:intergenic 35.0%
CATTAGGCATCAATTGATGA+AGG - chr7_4:10696805-10696824 None:intergenic 35.0%
TCATCAATTGATGCCTAATG+AGG + chr7_4:10696805-10696824 Msa1139880:CDS 35.0%
CTCCAATGTTATCACCAAAT+TGG + chr7_4:10696975-10696994 Msa1139880:CDS 35.0%
! TCCCAATTTGGTGATAACAT+TGG - chr7_4:10696980-10696999 None:intergenic 35.0%
AATAACAATGATGCTGTCAC+TGG + chr7_4:10697010-10697029 Msa1139880:CDS 35.0%
TCAAGAACGAATCTATCATC+AGG + chr7_4:10697055-10697074 Msa1139880:CDS 35.0%
! AATCTTTGGAAAATCATGGG+AGG + chr7_4:10697221-10697240 Msa1139880:CDS 35.0%
! TAGGTGAAGGGTTTTCATTT+TGG - chr7_4:10695763-10695782 None:intergenic 35.0%
!!! CCGTGTAAAAAGGTGTTTTT+AGG + chr7_4:10695878-10695897 Msa1139880:five_prime_UTR 35.0%
! CCTAAAGAGCAATAAGTTGA+AGG - chr7_4:10696023-10696042 None:intergenic 35.0%
CCTTCAACTTATTGCTCTTT+AGG + chr7_4:10696020-10696039 Msa1139880:CDS 35.0%
GGGTAAGAAAAGTAAAAGGA+GGG - chr7_4:10696094-10696113 None:intergenic 35.0%
TCAGGGGTAAGAAAAGTAAA+AGG - chr7_4:10696098-10696117 None:intergenic 35.0%
!!! AGAAGAAGCATTTTGATCAG+GGG - chr7_4:10696114-10696133 None:intergenic 35.0%
TGATGAGTTGTTTGTGTGTA+GGG - chr7_4:10696212-10696231 None:intergenic 35.0%
ATGATGAGTTGTTTGTGTGT+AGG - chr7_4:10696213-10696232 None:intergenic 35.0%
TTCGATCTTCAAGACATGTT+AGG + chr7_4:10696458-10696477 Msa1139880:CDS 35.0%
TCGATCTTCAAGACATGTTA+GGG + chr7_4:10696459-10696478 Msa1139880:CDS 35.0%
ATCTTCTTCATCTCCTTCAT+CGG - chr7_4:10696621-10696640 None:intergenic 35.0%
CATTAGGCATCAATTGATGA+AGG - chr7_4:10696805-10696824 None:intergenic 35.0%
TCATCAATTGATGCCTAATG+AGG + chr7_4:10696805-10696824 Msa1139880:CDS 35.0%
CTCCAATGTTATCACCAAAT+TGG + chr7_4:10696975-10696994 Msa1139880:CDS 35.0%
! TCCCAATTTGGTGATAACAT+TGG - chr7_4:10696980-10696999 None:intergenic 35.0%
AATAACAATGATGCTGTCAC+TGG + chr7_4:10697010-10697029 Msa1139880:CDS 35.0%
TCAAGAACGAATCTATCATC+AGG + chr7_4:10697055-10697074 Msa1139880:CDS 35.0%
! AATCTTTGGAAAATCATGGG+AGG + chr7_4:10697221-10697240 Msa1139880:CDS 35.0%
!! TTATCTGTGTGTGATGGACA+AGG - chr7_4:10695674-10695693 None:intergenic 40.0%
GGGGTAAGAAAAGTAAAAGG+AGG - chr7_4:10696095-10696114 None:intergenic 40.0%
!! GGTGTTGTTAGTGTTGTTGT+TGG - chr7_4:10696141-10696160 None:intergenic 40.0%
!! GCCCAATTTGTTAGTGTATC+AGG - chr7_4:10696251-10696270 None:intergenic 40.0%
! TTCTTAGCTGGTTTCTTGAC+AGG - chr7_4:10696356-10696375 None:intergenic 40.0%
AGAAACCAGCTAAGAAAGAC+AGG + chr7_4:10696360-10696379 Msa1139880:CDS 40.0%
AGCAAGATTCACACATCTCA+AGG + chr7_4:10696386-10696405 Msa1139880:CDS 40.0%
TCAAGGTTTGAGAGACAGAA+GGG + chr7_4:10696403-10696422 Msa1139880:CDS 40.0%
AGCAAGAACAACATAGCTTC+TGG + chr7_4:10696554-10696573 Msa1139880:CDS 40.0%
! AAAGATGAAAAGGGCACAGA+AGG + chr7_4:10696655-10696674 Msa1139880:CDS 40.0%
TTCGAAGACGAAGGATTCTA+GGG + chr7_4:10696682-10696701 Msa1139880:CDS 40.0%
AGAAAACAAGAGCAAGAGCA+AGG + chr7_4:10696705-10696724 Msa1139880:CDS 40.0%
GAAAACAAGAGCAAGAGCAA+GGG + chr7_4:10696706-10696725 Msa1139880:CDS 40.0%
!! TTATCTGTGTGTGATGGACA+AGG - chr7_4:10695674-10695693 None:intergenic 40.0%
GGGGTAAGAAAAGTAAAAGG+AGG - chr7_4:10696095-10696114 None:intergenic 40.0%
!! GGTGTTGTTAGTGTTGTTGT+TGG - chr7_4:10696141-10696160 None:intergenic 40.0%
!! GCCCAATTTGTTAGTGTATC+AGG - chr7_4:10696251-10696270 None:intergenic 40.0%
! TTCTTAGCTGGTTTCTTGAC+AGG - chr7_4:10696356-10696375 None:intergenic 40.0%
AGAAACCAGCTAAGAAAGAC+AGG + chr7_4:10696360-10696379 Msa1139880:CDS 40.0%
AGCAAGATTCACACATCTCA+AGG + chr7_4:10696386-10696405 Msa1139880:CDS 40.0%
TCAAGGTTTGAGAGACAGAA+GGG + chr7_4:10696403-10696422 Msa1139880:CDS 40.0%
AGCAAGAACAACATAGCTTC+TGG + chr7_4:10696554-10696573 Msa1139880:CDS 40.0%
! AAAGATGAAAAGGGCACAGA+AGG + chr7_4:10696655-10696674 Msa1139880:CDS 40.0%
TTCGAAGACGAAGGATTCTA+GGG + chr7_4:10696682-10696701 Msa1139880:CDS 40.0%
AGAAAACAAGAGCAAGAGCA+AGG + chr7_4:10696705-10696724 Msa1139880:CDS 40.0%
GAAAACAAGAGCAAGAGCAA+GGG + chr7_4:10696706-10696725 Msa1139880:CDS 40.0%
TAGGGAACAGAAATAGCTGC+TGG - chr7_4:10696194-10696213 None:intergenic 45.0%
CTATGCCTGTCTTTCTTAGC+TGG - chr7_4:10696368-10696387 None:intergenic 45.0%
CTCAAGGTTTGAGAGACAGA+AGG + chr7_4:10696402-10696421 Msa1139880:CDS 45.0%
AAGCCAGCAACACACTTGAA+TGG + chr7_4:10696489-10696508 Msa1139880:CDS 45.0%
TCTTCTTCGTCTTCCGATGA+AGG + chr7_4:10696605-10696624 Msa1139880:CDS 45.0%
GAAGGAATCTTCGAAGACGA+AGG + chr7_4:10696673-10696692 Msa1139880:CDS 45.0%
CTTCGAAGACGAAGGATTCT+AGG + chr7_4:10696681-10696700 Msa1139880:CDS 45.0%
TAGGGAACAGAAATAGCTGC+TGG - chr7_4:10696194-10696213 None:intergenic 45.0%
CTATGCCTGTCTTTCTTAGC+TGG - chr7_4:10696368-10696387 None:intergenic 45.0%
CTCAAGGTTTGAGAGACAGA+AGG + chr7_4:10696402-10696421 Msa1139880:CDS 45.0%
AAGCCAGCAACACACTTGAA+TGG + chr7_4:10696489-10696508 Msa1139880:CDS 45.0%
TCTTCTTCGTCTTCCGATGA+AGG + chr7_4:10696605-10696624 Msa1139880:CDS 45.0%
GAAGGAATCTTCGAAGACGA+AGG + chr7_4:10696673-10696692 Msa1139880:CDS 45.0%
CTTCGAAGACGAAGGATTCT+AGG + chr7_4:10696681-10696700 Msa1139880:CDS 45.0%
!! GTGTGATGGACAAGGCTCAT+GGG - chr7_4:10695666-10695685 None:intergenic 50.0%
!! TGTGTGATGGACAAGGCTCA+TGG - chr7_4:10695667-10695686 None:intergenic 50.0%
GAGCCATTCAAGTGTGTTGC+TGG - chr7_4:10696495-10696514 None:intergenic 50.0%
!! GTGTGATGGACAAGGCTCAT+GGG - chr7_4:10695666-10695685 None:intergenic 50.0%
!! TGTGTGATGGACAAGGCTCA+TGG - chr7_4:10695667-10695686 None:intergenic 50.0%
GAGCCATTCAAGTGTGTTGC+TGG - chr7_4:10696495-10696514 None:intergenic 50.0%
GATGGACAAGGCTCATGGGT+TGG - chr7_4:10695662-10695681 None:intergenic 55.0%
GACTTTCCATCGAGATCGCG+AGG + chr7_4:10696429-10696448 Msa1139880:CDS 55.0%
GAACTTCCTCGCGATCTCGA+TGG - chr7_4:10696438-10696457 None:intergenic 55.0%
GATGGACAAGGCTCATGGGT+TGG - chr7_4:10695662-10695681 None:intergenic 55.0%
GACTTTCCATCGAGATCGCG+AGG + chr7_4:10696429-10696448 Msa1139880:CDS 55.0%
GAACTTCCTCGCGATCTCGA+TGG - chr7_4:10696438-10696457 None:intergenic 55.0%
GCTTCTGGCGATGACGACGA+TGG + chr7_4:10696569-10696588 Msa1139880:CDS 60.0%
GCTTCTGGCGATGACGACGA+TGG + chr7_4:10696569-10696588 Msa1139880:CDS 60.0%
Chromosome Type Strat End Strand Name
chr7_4 gene 10695658 10697272 10695658 ID=Msa1139880;Name=Msa1139880
chr7_4 mRNA 10695658 10697272 10695658 ID=Msa1139880-mRNA-1;Parent=Msa1139880;Name=Msa1139880-mRNA-1;_AED=0.09;_eAED=0.09;_QI=356|0|0.5|1|1|1|2|0|378
chr7_4 exon 10695658 10697076 10695658 ID=Msa1139880-mRNA-1:exon:2615;Parent=Msa1139880-mRNA-1
chr7_4 exon 10697199 10697272 10697199 ID=Msa1139880-mRNA-1:exon:2616;Parent=Msa1139880-mRNA-1
chr7_4 five_prime_UTR 10695658 10696013 10695658 ID=Msa1139880-mRNA-1:five_prime_utr;Parent=Msa1139880-mRNA-1
chr7_4 CDS 10696014 10697076 10696014 ID=Msa1139880-mRNA-1:cds;Parent=Msa1139880-mRNA-1
chr7_4 CDS 10697199 10697272 10697199 ID=Msa1139880-mRNA-1:cds;Parent=Msa1139880-mRNA-1
Gene Sequence

>Msa1139880

ATGTTTCCTTCAACTTATTGCTCTTTAGGTCCTTACCCTTGTTATAATCCTAATTCATCTTCTTCTTCACACTCATACCCTCCTTTTACTTTTCTTACCCCTGATCAAAATGCTTCTTCTAACACCAACAACAACACTAACAACACCAACAACAACATCAACAACTTTCTTCATGATCCAGCAGCTATTTCTGTTCCCTACACACAAACAACTCATCATCATGTTCCAATTAATCCTGATACACTAACAAATTGGGCTGTTGCTGATTATGCAGCTATGTTGAAACAAGATCTAAGTGGTTCTTCTCATTACAATCTCTCAAATTTGCTCACAAAGAAACCTGTCAAGAAACCAGCTAAGAAAGACAGGCATAGCAAGATTCACACATCTCAAGGTTTGAGAGACAGAAGGGTGAGACTTTCCATCGAGATCGCGAGGAAGTTCTTCGATCTTCAAGACATGTTAGGGTTTGACAAAGCCAGCAACACACTTGAATGGCTCTTTAATAAATCAAAAGAAGCAATTGAGGAGTTAACTAGAAGCAAGAACAACATAGCTTCTGGCGATGACGACGATGGTGATCATAGCTTCTCTTCTTCGTCTTCCGATGAAGGAGATGAAGAAGATTATACTAAAGATAGAAAGATGAAAAGGGCACAGAAGGAATCTTCGAAGACGAAGGATTCTAGGGAGAAAACAAGAGCAAGAGCAAGGGAAAGAGCAAGTGAGATGAAGATGCAAGAAGATTTGAAAGAAAAATATCCTGAAATTGATCAAAATCCACAAATCCTTCATCAATTGATGCCTAATGAGGATGAAACTTCAAAATCACTTCAAAGAGATGATATCTTTAACTTCATTGAGGAATCTATTGTGATTAAGAGAAAGTTGAAGAAATCTTCTTCTCATCATTACCAACAAAACACTAATTTGATGCCTAATAATAGTGATTCTCATGACTCTCCAATGTTATCACCAAATTGGGATGCTAATAATAATAACAATGATGCTGTCACTGGAAGATCCAACTTTTCTGCAATATCAAGAACGAATCTATCATCAGGTTTAAACTTTTTAGGGCTTCAAATCTTTGGAAAATCATGGGAGGATTGCAATACCAGTCCAAATCGATATTAG
Protein sequence

>Msa1139880

MFPSTYCSLGPYPCYNPNSSSSSHSYPPFTFLTPDQNASSNTNNNTNNTNNNINNFLHDPAAISVPYTQTTHHHVPINPDTLTNWAVADYAAMLKQDLSGSSHYNLSNLLTKKPVKKPAKKDRHSKIHTSQGLRDRRVRLSIEIARKFFDLQDMLGFDKASNTLEWLFNKSKEAIEELTRSKNNIASGDDDDGDHSFSSSSSDEGDEEDYTKDRKMKRAQKESSKTKDSREKTRARARERASEMKMQEDLKEKYPEIDQNPQILHQLMPNEDETSKSLQRDDIFNFIEESIVIKRKLKKSSSHHYQQNTNLMPNNSDSHDSPMLSPNWDANNNNNDAVTGRSNFSAISRTNLSSGLNFLGLQIFGKSWEDCNTSPNRY*