AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0118400


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0118400 MtrunA17_Chr1g0175821 46.316 190 92 1 6 185 26 215 5.78e-47 153
Msa0118400 MtrunA17_Chr1g0175811 39.819 221 83 6 1 186 1 206 1.01e-35 124
Msa0118400 MtrunA17_Chr1g0175791 45.963 161 59 3 8 141 67 226 2.01e-28 106
Msa0118400 MtrunA17_Chr5g0420601 33.333 201 96 5 21 186 85 282 1.39e-18 80.9
Msa0118400 MtrunA17_Chr1g0175781 31.250 208 94 5 21 186 90 290 7.47e-16 73.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 36 sgRNAs with CRISPR-Local

Find 38 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCAACTTGTTCAAGGTTTA+AGG 0.133169 1_3:-41150593 None:intergenic
CTTGTAAACAATTCCTTCTT+TGG 0.221022 1_3:-41150451 None:intergenic
CTTCGAAAAGAGTGGATTAA+TGG 0.276341 1_3:+41150374 Msa0118400:CDS
GGAGCAAAAGGTTTGTGATT+TGG 0.304158 1_3:+41150245 Msa0118400:CDS
AAGTTGGAGGCTGAAATTAA+AGG 0.305610 1_3:+41150471 Msa0118400:CDS
CTTGTTCAAGGTTTAAGGTT+AGG 0.370602 1_3:-41150588 None:intergenic
ATGGCTACTCTACATCAATC+TGG 0.390287 1_3:+41150106 Msa0118400:CDS
GCAAGCAACACCCGTTCAAT+TGG 0.411385 1_3:+41150203 Msa0118400:CDS
AGTTGGAGGCTGAAATTAAA+GGG 0.412362 1_3:+41150472 Msa0118400:CDS
TGCTCGTTGTATGGAGCAAA+AGG 0.418843 1_3:+41150233 Msa0118400:CDS
GGAAAATTGCACACCAAAGA+AGG 0.418990 1_3:+41150438 Msa0118400:CDS
GGTCTCGATTGCTCGTTGTA+TGG 0.434380 1_3:+41150224 Msa0118400:CDS
CACTAAAATTGAAGAATTGA+AGG 0.435268 1_3:+41150500 Msa0118400:CDS
AGAAGGAATTGTTTACAAGT+TGG 0.457133 1_3:+41150455 Msa0118400:CDS
GTGATTTGGAAATGAGCTGC+AGG 0.481810 1_3:+41150259 Msa0118400:CDS
AGGAATTGTTTACAAGTTGG+AGG 0.487776 1_3:+41150458 Msa0118400:CDS
AAAGGTTCATGAGGCATATG+AGG 0.490646 1_3:+41150149 Msa0118400:CDS
ATTGTAGTTCAGCACAATGT+TGG 0.502018 1_3:+41150528 Msa0118400:CDS
CTGAATCTGAGAATAACATA+GGG 0.510118 1_3:+41150322 Msa0118400:CDS
TCTGAATCTGAGAATAACAT+AGG 0.510558 1_3:+41150321 Msa0118400:CDS
GAGTGGATTAATGGAAAGAA+CGG 0.524492 1_3:+41150383 Msa0118400:CDS
GCACAATGTTGGCTTTAATG+AGG 0.525326 1_3:+41150539 Msa0118400:CDS
TCATGAGGCATATGAGGAGT+GGG 0.527499 1_3:+41150155 Msa0118400:CDS
GCAATCGAGACCAATTGAAC+GGG 0.527802 1_3:-41150213 None:intergenic
GTAGAGTAGCCATTCTCAGC+TGG 0.533250 1_3:-41150096 None:intergenic
AAGACTACACCAGCTGAGAA+TGG 0.552919 1_3:+41150087 Msa0118400:CDS
TGGCTACTCTACATCAATCT+GGG 0.557094 1_3:+41150107 Msa0118400:CDS
GAGTTAATCTTCGAAAAGAG+TGG 0.570774 1_3:+41150366 Msa0118400:CDS
AGAATGGATCCCAAGAGATG+TGG 0.577195 1_3:+41150042 None:intergenic
TATCACAGAAAAGGTTCATG+AGG 0.579514 1_3:+41150140 Msa0118400:CDS
TTCATGAGGCATATGAGGAG+TGG 0.581486 1_3:+41150154 Msa0118400:CDS
CGGGTGTTGCTTGCAAAACA+CGG 0.601763 1_3:-41150194 None:intergenic
AGCAATCGAGACCAATTGAA+CGG 0.624783 1_3:-41150214 None:intergenic
TGATTTGGAAATGAGCTGCA+GGG 0.643574 1_3:+41150260 Msa0118400:CDS
CATGAGGCATATGAGGAGTG+GGG 0.686751 1_3:+41150156 Msa0118400:CDS
TGAATCTGAGAATAACATAG+GGG 0.713948 1_3:+41150323 Msa0118400:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGACAATTTTATCACAGAAA+AGG + chr1_3:41150131-41150150 Msa0118400:CDS 25.0%
!! AATGAAAACTCCAAGATTTT+AGG + chr1_3:41150417-41150436 Msa0118400:CDS 25.0%
! CACTAAAATTGAAGAATTGA+AGG + chr1_3:41150500-41150519 Msa0118400:CDS 25.0%
TCTGAATCTGAGAATAACAT+AGG + chr1_3:41150321-41150340 Msa0118400:CDS 30.0%
CTGAATCTGAGAATAACATA+GGG + chr1_3:41150322-41150341 Msa0118400:CDS 30.0%
TGAATCTGAGAATAACATAG+GGG + chr1_3:41150323-41150342 Msa0118400:CDS 30.0%
! GTGCAATTTTCCTAAAATCT+TGG - chr1_3:41150430-41150449 None:intergenic 30.0%
CTTGTAAACAATTCCTTCTT+TGG - chr1_3:41150454-41150473 None:intergenic 30.0%
AGAAGGAATTGTTTACAAGT+TGG + chr1_3:41150455-41150474 Msa0118400:CDS 30.0%
TATCACAGAAAAGGTTCATG+AGG + chr1_3:41150140-41150159 Msa0118400:CDS 35.0%
GAGTTAATCTTCGAAAAGAG+TGG + chr1_3:41150366-41150385 Msa0118400:CDS 35.0%
!! CTTCGAAAAGAGTGGATTAA+TGG + chr1_3:41150374-41150393 Msa0118400:CDS 35.0%
!! GAGTGGATTAATGGAAAGAA+CGG + chr1_3:41150383-41150402 Msa0118400:CDS 35.0%
! AGGAATTGTTTACAAGTTGG+AGG + chr1_3:41150458-41150477 Msa0118400:CDS 35.0%
AAGTTGGAGGCTGAAATTAA+AGG + chr1_3:41150471-41150490 Msa0118400:CDS 35.0%
AGTTGGAGGCTGAAATTAAA+GGG + chr1_3:41150472-41150491 Msa0118400:CDS 35.0%
ATTGTAGTTCAGCACAATGT+TGG + chr1_3:41150528-41150547 Msa0118400:CDS 35.0%
! CTACGTTTTCCACATCTCTT+GGG - chr1_3:41150054-41150073 None:intergenic 40.0%
ATGGCTACTCTACATCAATC+TGG + chr1_3:41150106-41150125 Msa0118400:CDS 40.0%
TGGCTACTCTACATCAATCT+GGG + chr1_3:41150107-41150126 Msa0118400:CDS 40.0%
AAAGGTTCATGAGGCATATG+AGG + chr1_3:41150149-41150168 Msa0118400:CDS 40.0%
AGCAATCGAGACCAATTGAA+CGG - chr1_3:41150217-41150236 None:intergenic 40.0%
GGAGCAAAAGGTTTGTGATT+TGG + chr1_3:41150245-41150264 Msa0118400:CDS 40.0%
! TGATTTGGAAATGAGCTGCA+GGG + chr1_3:41150260-41150279 Msa0118400:CDS 40.0%
GGAAAATTGCACACCAAAGA+AGG + chr1_3:41150438-41150457 Msa0118400:CDS 40.0%
!! GCACAATGTTGGCTTTAATG+AGG + chr1_3:41150539-41150558 Msa0118400:CDS 40.0%
! GCTACGTTTTCCACATCTCT+TGG - chr1_3:41150055-41150074 None:intergenic 45.0%
AAGACTACACCAGCTGAGAA+TGG + chr1_3:41150087-41150106 Msa0118400:CDS 45.0%
TTCATGAGGCATATGAGGAG+TGG + chr1_3:41150154-41150173 Msa0118400:CDS 45.0%
TCATGAGGCATATGAGGAGT+GGG + chr1_3:41150155-41150174 Msa0118400:CDS 45.0%
GCAATCGAGACCAATTGAAC+GGG - chr1_3:41150216-41150235 None:intergenic 45.0%
TGCTCGTTGTATGGAGCAAA+AGG + chr1_3:41150233-41150252 Msa0118400:CDS 45.0%
! GTGATTTGGAAATGAGCTGC+AGG + chr1_3:41150259-41150278 Msa0118400:CDS 45.0%
GTAGAGTAGCCATTCTCAGC+TGG - chr1_3:41150099-41150118 None:intergenic 50.0%
CATGAGGCATATGAGGAGTG+GGG + chr1_3:41150156-41150175 Msa0118400:CDS 50.0%
!! CGGGTGTTGCTTGCAAAACA+CGG - chr1_3:41150197-41150216 None:intergenic 50.0%
GCAAGCAACACCCGTTCAAT+TGG + chr1_3:41150203-41150222 Msa0118400:CDS 50.0%
GGTCTCGATTGCTCGTTGTA+TGG + chr1_3:41150224-41150243 Msa0118400:CDS 50.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 41150045 41150605 41150045 ID=Msa0118400;Name=Msa0118400
chr1_3 mRNA 41150045 41150605 41150045 ID=Msa0118400-mRNA-1;Parent=Msa0118400;Name=Msa0118400-mRNA-1;_AED=0.34;_eAED=0.34;_QI=0|-1|0|1|-1|1|1|0|186
chr1_3 exon 41150045 41150605 41150045 ID=Msa0118400-mRNA-1:exon:12475;Parent=Msa0118400-mRNA-1
chr1_3 CDS 41150045 41150605 41150045 ID=Msa0118400-mRNA-1:cds;Parent=Msa0118400-mRNA-1
Gene Sequence

>Msa0118400

ATGGATCCCAAGAGATGTGGAAAACGTAGCAAAAGAAAGAGAAAGACTACACCAGCTGAGAATGGCTACTCTACATCAATCTGGGATGACAATTTTATCACAGAAAAGGTTCATGAGGCATATGAGGAGTGGGGATTAGAAAAGTGCTTCCGTGTTTTGCAAGCAACACCCGTTCAATTGGTCTCGATTGCTCGTTGTATGGAGCAAAAGGTTTGTGATTTGGAAATGAGCTGCAGGGCTCACATTGAAGAGAATACTAAATTGAAAAAGAAGTTGTCTGAATCTGAGAATAACATAGGGGAATTAAAGAGAAAGTTCGAAGAGTTAATCTTCGAAAAGAGTGGATTAATGGAAAGAACGGAAGACTTGAAGAATGAAAACTCCAAGATTTTAGGAAAATTGCACACCAAAGAAGGAATTGTTTACAAGTTGGAGGCTGAAATTAAAGGGCTGAACACTAAAATTGAAGAATTGAAGGATGAAATTGTAGTTCAGCACAATGTTGGCTTTAATGAGGCTGTCATACAATTTTTAACTTCAATACCTAACCTTAAACCTTGA

Protein sequence

>Msa0118400

MDPKRCGKRSKRKRKTTPAENGYSTSIWDDNFITEKVHEAYEEWGLEKCFRVLQATPVQLVSIARCMEQKVCDLEMSCRAHIEENTKLKKKLSESENNIGELKRKFEELIFEKSGLMERTEDLKNENSKILGKLHTKEGIVYKLEAEIKGLNTKIEELKDEIVVQHNVGFNEAVIQFLTSIPNLKP*