AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1141850

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1141850 MtrunA17_Chr7g0223631 86.570 618 69 2 1 618 1 604 0.0 1077
Msa1141850 MtrunA17_Chr7g0223711 82.553 619 107 1 1 618 1 619 0.0 1057
Msa1141850 MtrunA17_Chr8g0350731 82.524 618 108 0 1 618 29 646 0.0 1048
Msa1141850 MtrunA17_Chr7g0223751 79.450 618 127 0 1 618 1 618 0.0 1023
Msa1141850 MtrunA17_Chr7g0223701 78.469 627 126 3 1 618 1 627 0.0 999
Msa1141850 MtrunA17_Chr7g0223621 76.961 612 141 0 1 612 1 612 0.0 970
Msa1141850 MtrunA17_Chr7g0223731 75.693 613 149 0 1 613 1 613 0.0 958
Msa1141850 MtrunA17_Chr7g0223031 75.327 612 151 0 1 612 1 612 0.0 956
Msa1141850 MtrunA17_Chr7g0248131 74.639 623 153 4 1 618 1 623 0.0 939
Msa1141850 MtrunA17_Chr7g0223741 74.372 597 152 1 1 596 1 597 0.0 931
Msa1141850 MtrunA17_Chr7g0248151 74.235 621 157 2 1 618 1 621 0.0 921
Msa1141850 MtrunA17_Chr7g0223681 84.659 528 81 0 91 618 2 529 0.0 905
Msa1141850 MtrunA17_Chr7g0223781 68.352 613 191 2 1 612 1 611 0.0 856
Msa1141850 MtrunA17_Chr2g0293581 67.581 620 197 4 1 616 1 620 0.0 852
Msa1141850 MtrunA17_Chr7g0221661 67.758 611 196 1 1 610 1 611 0.0 847
Msa1141850 MtrunA17_Chr7g0223181 67.541 610 193 4 1 607 1 608 0.0 842
Msa1141850 MtrunA17_Chr7g0223541 73.810 546 142 1 74 618 31 576 0.0 841
Msa1141850 MtrunA17_Chr7g0223801 67.159 609 199 1 1 609 1 608 0.0 840
Msa1141850 MtrunA17_Chr4g0031321 67.049 610 196 4 1 607 1 608 0.0 834
Msa1141850 MtrunA17_Chr5g0427901 65.600 625 203 2 1 613 1 625 0.0 833
Msa1141850 MtrunA17_Chr7g0262821 66.023 621 206 4 1 618 1 619 0.0 833
Msa1141850 MtrunA17_Chr4g0031301 66.885 610 197 4 1 607 1 608 0.0 833
Msa1141850 MtrunA17_Chr7g0223721 66.450 614 204 2 1 612 1 614 0.0 833
Msa1141850 MtrunA17_Chr7g0233311 66.016 615 206 2 1 612 1 615 0.0 831
Msa1141850 MtrunA17_Chr7g0223291 65.378 621 210 4 1 618 1 619 0.0 826
Msa1141850 MtrunA17_Chr2g0298851 66.667 612 200 4 1 609 1 611 0.0 825
Msa1141850 MtrunA17_Chr2g0298861 65.008 623 204 4 1 609 1 623 0.0 821
Msa1141850 MtrunA17_Chr7g0223851 65.049 618 214 2 1 616 10 627 0.0 815
Msa1141850 MtrunA17_Chr4g0040931 64.812 611 213 2 1 609 1 611 0.0 810
Msa1141850 MtrunA17_Chr4g0073741 64.251 621 216 5 1 618 1 618 0.0 806
Msa1141850 MtrunA17_Chr7g0225671 64.610 616 213 4 3 616 12 624 0.0 796
Msa1141850 MtrunA17_Chr7g0217971 65.261 593 182 1 1 593 1 569 0.0 794
Msa1141850 MtrunA17_Chr2g0287631 65.196 612 210 3 1 609 1 612 0.0 787
Msa1141850 MtrunA17_Chr7g0223821 64.332 614 212 3 1 608 1 613 0.0 776
Msa1141850 MtrunA17_Chr2g0296391 70.265 528 155 2 1 526 1 528 0.0 762
Msa1141850 MtrunA17_Chr4g0012541 62.233 609 219 3 1 607 1 600 0.0 755
Msa1141850 MtrunA17_Chr7g0233301 61.111 612 204 3 1 609 1 581 0.0 751
Msa1141850 MtrunA17_Chr7g0223831 60.778 617 210 5 1 614 1 588 0.0 738
Msa1141850 MtrunA17_Chr8g0390821 58.824 663 204 8 1 609 1 648 0.0 738
Msa1141850 MtrunA17_Chr7g0215261 59.871 618 209 5 1 614 1 583 0.0 724
Msa1141850 MtrunA17_Chr6g0462601 58.101 611 252 3 1 609 1 609 0.0 702
Msa1141850 MtrunA17_Chr6g0462681 59.552 581 231 3 1 579 1 579 0.0 699
Msa1141850 MtrunA17_Chr2g0293601 66.602 518 137 3 1 516 1 484 0.0 699
Msa1141850 MtrunA17_Chr7g0228281 57.929 618 213 5 1 616 62 634 0.0 697
Msa1141850 MtrunA17_Chr6g0462711 59.413 579 227 4 8 579 9 586 0.0 682
Msa1141850 MtrunA17_Chr2g0331381 63.722 532 132 3 10 541 7 477 0.0 681
Msa1141850 MtrunA17_Chr6g0462641 57.436 585 240 4 1 578 1 583 0.0 668
Msa1141850 MtrunA17_Chr6g0462771 58.131 578 233 4 8 578 8 583 0.0 664
Msa1141850 MtrunA17_Chr2g0284681 58.214 560 223 6 9 559 8 565 0.0 651
Msa1141850 MtrunA17_Chr8g0388671 54.499 589 257 8 7 590 36 618 0.0 628
Msa1141850 MtrunA17_Chr8g0388761 53.990 589 260 8 7 590 36 618 0.0 627
Msa1141850 MtrunA17_Chr1g0166551 53.146 604 170 4 9 610 5 497 0.0 602
Msa1141850 MtrunA17_Chr2g0293561 74.064 374 97 0 1 374 1 374 0.0 600
Msa1141850 MtrunA17_Chr6g0462611 57.002 507 216 2 74 579 22 527 0.0 584
Msa1141850 MtrunA17_Chr2g0299921 70.558 394 112 2 3 396 1023 1412 0.0 570
Msa1141850 MtrunA17_Chr2g0299921 68.528 394 119 3 3 395 1407 1796 3.59e-170 529
Msa1141850 MtrunA17_Chr2g0299791 69.409 389 115 2 1 389 1034 1418 0.0 554
Msa1141850 MtrunA17_Chr2g0299851 67.692 390 122 2 1 390 1030 1415 0.0 553
Msa1141850 MtrunA17_Chr2g0299821 53.333 540 194 6 1 538 1 484 0.0 549
Msa1141850 MtrunA17_Chr2g0293271 58.756 434 109 3 21 454 43 406 1.17e-173 499
Msa1141850 MtrunA17_Chr2g0299881 66.327 392 124 4 2 389 1031 1418 1.43e-170 524
Msa1141850 MtrunA17_Chr2g0299881 58.209 67 28 0 184 250 1409 1475 1.04e-17 87.8
Msa1141850 MtrunA17_Chr6g0462861 46.271 590 285 9 1 579 1 569 4.92e-166 489
Msa1141850 MtrunA17_Chr2g0279901 47.842 556 266 11 10 558 57 595 1.63e-163 483
Msa1141850 MtrunA17_Chr4g0049501 46.763 556 273 10 10 558 54 593 4.52e-160 474
Msa1141850 MtrunA17_Chr2g0277021 45.034 584 297 12 10 586 72 638 4.74e-153 457
Msa1141850 MtrunA17_Chr1g0151991 71.478 291 83 0 1 291 1 291 5.26e-152 439
Msa1141850 MtrunA17_Chr7g0245521 73.993 273 71 0 1 273 1 273 1.91e-151 437
Msa1141850 MtrunA17_Chr7g0245531 60.064 313 124 1 305 616 2 314 6.31e-125 371
Msa1141850 MtrunA17_Chr7g0223761 52.561 371 133 3 241 609 3 332 3.65e-122 365
Msa1141850 MtrunA17_Chr7g0223671 85.405 185 27 0 74 258 8 192 1.38e-114 340
Msa1141850 MtrunA17_Chr1g0152001 58.108 296 122 2 315 609 1 295 4.81e-108 328
Msa1141850 MtrunA17_Chr1g0188601 75.393 191 47 0 92 282 1 191 8.03e-103 310
Msa1141850 MtrunA17_Chr6g0462631 50.000 330 116 5 91 416 13 297 2.16e-100 307
Msa1141850 MtrunA17_Chr6g0462751 47.813 343 119 4 74 413 19 304 7.36e-97 299
Msa1141850 MtrunA17_Chr1g0188611 54.608 293 124 3 322 612 1 286 7.41e-86 270
Msa1141850 MtrunA17_Chr5g0394391 34.791 503 302 12 8 493 28 521 2.47e-81 267
Msa1141850 MtrunA17_Chr7g0223771 74.026 154 40 0 93 246 1 154 4.20e-79 247
Msa1141850 MtrunA17_Chr6g0462791 65.730 178 60 1 109 286 2 178 1.81e-76 241
Msa1141850 MtrunA17_Chr4g0004331 34.562 434 263 6 10 428 4 431 4.38e-74 254
Msa1141850 MtrunA17_Chr2g0331901 33.793 435 271 5 10 431 4 434 7.56e-74 253
Msa1141850 MtrunA17_Chr3g0098761 33.810 420 272 5 10 428 4 418 1.07e-70 243
Msa1141850 MtrunA17_Chr5g0444001 33.333 429 276 6 10 432 4 428 1.06e-69 235
Msa1141850 MtrunA17_Chr4g0046881 74.483 145 34 1 131 275 10 151 5.69e-69 220
Msa1141850 MtrunA17_Chr7g0223861 74.046 131 34 0 78 208 4 134 2.01e-63 205
Msa1141850 MtrunA17_Chr4g0064621 33.973 521 309 14 8 502 139 650 4.43e-63 221
Msa1141850 MtrunA17_Chr3g0120101 27.692 520 334 12 10 493 27 540 2.94e-58 210
Msa1141850 MtrunA17_Chr6g0462701 53.333 180 60 1 91 270 13 168 1.34e-54 183
Msa1141850 MtrunA17_Chr4g0060241 30.137 511 317 16 8 498 19 509 3.99e-53 191
Msa1141850 MtrunA17_Chr5g0394221 40.357 280 160 6 291 566 61 337 3.64e-52 183
Msa1141850 MtrunA17_Chr7g0245541 46.000 200 107 1 417 615 1 200 1.50e-48 168
Msa1141850 MtrunA17_Chr6g0481391 64.754 122 40 1 38 159 13 131 3.20e-47 162
Msa1141850 MtrunA17_Chr5g0442171 34.535 333 190 10 170 487 1 320 1.78e-46 166
Msa1141850 MtrunA17_Chr4g0046871 74.757 103 26 0 333 435 3 105 6.13e-46 157
Msa1141850 MtrunA17_Chr6g0462691 52.113 142 67 1 322 462 1 142 1.25e-42 150
Msa1141850 MtrunA17_Chr7g0223691 90.411 73 7 0 1 73 1 73 6.57e-42 145
Msa1141850 MtrunA17_Chr5g0394231 36.638 232 94 5 74 304 5 184 1.13e-41 149
Msa1141850 MtrunA17_Chr1g0188591 78.082 73 16 0 1 73 1 73 4.82e-33 122
Msa1141850 MtrunA17_Chr6g0481381 72.973 74 20 0 176 249 1 74 7.06e-31 119
Msa1141850 MtrunA17_Chr6g0481381 38.947 95 57 1 514 607 75 169 7.02e-11 61.6
Msa1141850 MtrunA17_Chr7g0223661 56.897 116 7 1 315 430 1 73 7.28e-31 115
Msa1141850 MtrunA17_Chr6g0462621 75.342 73 16 1 1 73 1 71 7.75e-30 112
Msa1141850 MtrunA17_Chr7g0223791 69.512 82 17 3 56 137 18 91 1.12e-27 110
Msa1141850 MtrunA17_Chr7g0223791 36.000 125 68 2 450 572 67 181 2.84e-15 74.3
Msa1141850 MtrunA17_Chr7g0223881 55.422 83 36 1 281 363 2 83 2.33e-24 97.1
Msa1141850 MtrunA17_Chr7g0221651 50.000 84 42 0 215 298 1 84 3.20e-23 94.0
Msa1141850 MtrunA17_Chr7g0223871 37.815 119 73 1 490 607 7 125 4.20e-17 78.6
Msa1141850 MtrunA17_Chr2g0284661 50.602 83 40 1 368 449 61 143 4.86e-17 78.2
Msa1141850 MtrunA17_Chr2g0284661 50.820 61 28 1 225 283 5 65 3.90e-11 61.2
Msa1141850 MtrunA17_Chr4g0064651 41.489 94 53 2 397 489 29 121 5.37e-15 72.0
Msa1141850 MtrunA17_Chr6g0462801 54.545 66 25 1 322 382 1 66 7.53e-15 69.7
Msa1141850 MtrunA17_Chr2g0299891 74.419 43 11 0 315 357 1 43 8.36e-15 69.7
Msa1141850 MtrunA17_Chr5g0442161 29.936 157 103 4 5 157 24 177 1.11e-12 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1141850 AT3G12580.1 66.828 621 201 4 1 618 1 619 0.0 841
Msa1141850 AT5G02500.1 66.066 610 202 4 1 607 1 608 0.0 827
Msa1141850 AT3G09440.1 66.230 610 201 4 1 607 1 608 0.0 819
Msa1141850 AT3G09440.4 66.230 610 201 4 1 607 1 608 0.0 819
Msa1141850 AT3G09440.2 66.230 610 201 4 1 607 1 608 0.0 819
Msa1141850 AT3G09440.3 66.230 610 201 4 1 607 1 608 0.0 819
Msa1141850 AT5G02490.1 65.246 610 207 4 1 607 1 608 0.0 817
Msa1141850 AT1G56410.1 63.607 621 219 5 1 618 1 617 0.0 800
Msa1141850 AT1G16030.1 63.446 621 221 5 1 618 1 618 0.0 793
Msa1141850 AT5G28540.1 54.290 606 266 8 7 607 35 634 0.0 642
Msa1141850 AT5G42020.1 54.290 606 266 8 7 607 35 634 0.0 641
Msa1141850 AT5G42020.3 54.290 606 266 8 7 607 40 639 0.0 639
Msa1141850 AT5G02500.2 75.321 389 94 1 1 389 1 387 0.0 620
Msa1141850 AT5G02500.2 37.634 93 58 0 515 607 386 478 8.23e-12 68.6
Msa1141850 AT1G09080.1 52.529 613 281 7 7 615 50 656 0.0 607
Msa1141850 AT1G09080.2 52.529 613 281 7 7 615 40 646 0.0 607
Msa1141850 AT5G42020.2 49.256 605 243 9 7 607 35 579 0.0 552
Msa1141850 AT5G09590.1 46.583 556 274 10 10 558 60 599 2.61e-157 468
Msa1141850 AT4G37910.1 47.122 556 271 10 10 558 55 594 1.50e-153 458
Msa1141850 AT4G37910.2 47.122 556 271 10 10 558 55 594 1.50e-153 458
Msa1141850 AT4G24280.1 46.320 557 277 11 10 559 81 622 9.16e-153 457
Msa1141850 AT5G49910.1 45.961 557 279 11 10 559 81 622 3.98e-151 453
Msa1141850 AT1G11660.4 29.248 612 355 11 10 560 4 598 7.71e-80 268
Msa1141850 AT1G11660.3 29.248 612 355 11 10 560 4 598 7.71e-80 268
Msa1141850 AT1G11660.1 29.248 612 355 11 10 560 4 598 7.71e-80 268
Msa1141850 AT1G79930.2 33.262 466 282 6 10 450 4 465 2.51e-76 259
Msa1141850 AT1G79930.1 33.262 466 282 6 10 450 4 465 6.34e-76 259
Msa1141850 AT1G79920.1 33.333 468 279 7 10 450 4 465 2.36e-75 255
Msa1141850 AT1G79920.3 33.333 468 279 7 10 450 4 465 2.39e-75 255
Msa1141850 AT1G79920.2 33.333 468 279 7 10 450 4 465 2.39e-75 255
Msa1141850 AT1G79920.4 33.333 468 279 7 10 450 4 465 2.39e-75 255
Msa1141850 AT1G11660.2 29.739 575 328 10 47 560 2 561 1.57e-73 250
Msa1141850 AT2G32120.1 32.943 513 313 12 8 506 29 524 6.49e-72 242
Msa1141850 AT2G32120.2 32.943 513 313 12 8 506 29 524 6.49e-72 242
Msa1141850 AT4G16660.1 27.810 525 334 13 6 493 23 539 5.03e-59 213
Msa1141850 AT4G16660.2 27.810 525 334 13 6 493 47 563 7.08e-59 212

Find 175 sgRNAs with CRISPR-Local

Find 226 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCTTACTGTGAAGTAATTT+TGG 0.095272 7_4:+15444140 Msa1141850:five_prime_UTR
TCACCCTCATAAACCTTAAT+TGG 0.121211 7_4:-15447252 None:intergenic
CTCAACCTCCTCAAGGCTTT+TGG 0.126998 7_4:-15446709 None:intergenic
TTTGAATTGGCTTCAAAATT+TGG 0.143460 7_4:-15445438 None:intergenic
TCTACTGTTGTGGCCCTTTA+AGG 0.152881 7_4:+15446189 Msa1141850:CDS
AACCCAATCAACACTGTATT+TGG 0.172484 7_4:+15445695 Msa1141850:CDS
TCTAGTCTCAATTGTCTTAT+AGG 0.193832 7_4:-15444545 None:intergenic
TCCTTGTTGGTGGTTCTTCT+AGG 0.229614 7_4:+15446944 Msa1141850:CDS
GTGATAATCCTTAGTGATTA+AGG 0.230689 7_4:+15444025 Msa1141850:five_prime_UTR
TGTAGTGTATAGTAGTATTT+TGG 0.234038 7_4:-15445401 None:intergenic
GCCAATTGTTTGTTTCAAAA+AGG 0.243615 7_4:-15444057 None:intergenic
TGGCCCTTTAAGGTCATTTC+CGG 0.243786 7_4:+15446199 Msa1141850:CDS
GCAGATGCAAAGAGGTTAAT+TGG 0.250254 7_4:+15446133 Msa1141850:intron
CTGTCTTGTTGATGCTAAAA+TGG 0.256497 7_4:+15446897 Msa1141850:CDS
CCTTGAAATTGTCTGCTTCC+TGG 0.258507 7_4:-15447488 None:intergenic
CCAATCTTCAGTCCCAATTA+AGG 0.259151 7_4:+15447239 Msa1141850:CDS
TAAATTAATCTTAATTAGTT+TGG 0.265380 7_4:+15444929 Msa1141850:five_prime_UTR
ATCAAACAAAGTGTTAATTT+AGG 0.283016 7_4:+15444658 Msa1141850:five_prime_UTR
TGATTGGAAGACTAAAATTA+AGG 0.292377 7_4:-15444585 None:intergenic
ATTACAATAACTAATGAAAA+TGG 0.292577 7_4:+15447432 Msa1141850:CDS
CTTACTCTTGTGTTGGAGTA+TGG 0.293550 7_4:+15445546 Msa1141850:CDS
TGTAATAACTGCCTCACTTT+AGG 0.312891 7_4:-15446970 None:intergenic
AGAGATTGCAGAGGCATTCT+TGG 0.315067 7_4:+15446318 Msa1141850:CDS
GAAAAGCATAAAGTGTAAAA+AGG 0.327768 7_4:-15444613 None:intergenic
TAAAGTGAGGCAGTTATTAC+AGG 0.333837 7_4:+15446972 Msa1141850:CDS
CCTGATGAGGCTGTTGCTTA+TGG 0.340665 7_4:+15447033 Msa1141850:CDS
CAAGTTTGAGCAATTGAATA+TGG 0.349032 7_4:+15446840 Msa1141850:CDS
AGCACCGGAAATGACCTTAA+AGG 0.349583 7_4:-15446203 None:intergenic
ACTAAAACTATTAATTTGTT+TGG 0.354670 7_4:-15444705 None:intergenic
GTAATCACTGCATTCTTAAC+AGG 0.355412 7_4:-15446346 None:intergenic
ATTCAAATCTTCTCTAGAAA+TGG 0.358210 7_4:+15445454 Msa1141850:five_prime_UTR
GGAAGATACCTTTGTGTTTG+TGG 0.363989 7_4:+15447689 Msa1141850:CDS
GTCGATGATGTTGTCCTTGT+TGG 0.366819 7_4:+15446931 Msa1141850:CDS
CAGCATCACCAATCAGCCTT+TGG 0.372766 7_4:-15445652 None:intergenic
ACATCTCTCAGCACCATGTT+TGG 0.373927 7_4:-15447102 None:intergenic
CTGCCTGTGAGAGGGCAAAA+AGG 0.378093 7_4:+15446734 Msa1141850:CDS
GTTCAAGAGGAAGAACAAAA+AGG 0.380579 7_4:+15446672 Msa1141850:CDS
AGTTGAAGCAAGAAATGCTT+TGG 0.381737 7_4:+15447536 Msa1141850:CDS
TTACCAAATACAGTGTTGAT+TGG 0.383287 7_4:-15445698 None:intergenic
TGCTTATGGTGCAGCTGTCC+AGG 0.386356 7_4:+15447047 Msa1141850:CDS
GTCACTTGGTATTGAATTGA+AGG 0.391810 7_4:+15447134 Msa1141850:CDS
CCATAAGCAACAGCCTCATC+AGG 0.392319 7_4:-15447033 None:intergenic
CCCAAAATTACTTCACAGTA+AGG 0.393135 7_4:-15444141 None:intergenic
TTAATTAGAACTAGACATAA+AGG 0.394210 7_4:+15444759 Msa1141850:five_prime_UTR
TTTCTAGAGAAGATTTGAAT+TGG 0.395670 7_4:-15445451 None:intergenic
CCTTAATTGGGACTGAAGAT+TGG 0.399757 7_4:-15447239 None:intergenic
AATATCACCTGCATCTTTAG+TGG 0.406579 7_4:-15446404 None:intergenic
TACCAAATACAGTGTTGATT+GGG 0.407934 7_4:-15445697 None:intergenic
ATCAATAGAACAAACTTGAT+TGG 0.409105 7_4:-15444462 None:intergenic
TGAGTACAAGAAGTATTTGT+TGG 0.409341 7_4:-15445037 None:intergenic
AGCATCACCAATCAGCCTTT+GGG 0.411059 7_4:-15445651 None:intergenic
ACTGCGGCGGCTCTTGCATA+TGG 0.414217 7_4:+15446463 Msa1141850:CDS
ACAAATTCCCAAAGGCTGAT+TGG 0.415818 7_4:+15445644 Msa1141850:CDS
AGTCCCAATTAAGGTTTATG+AGG 0.424356 7_4:+15447248 Msa1141850:CDS
CATATTCAATTGCTCAAACT+TGG 0.426433 7_4:-15446839 None:intergenic
TTTGATGTCAAGGCCACTGC+TGG 0.427311 7_4:+15446589 Msa1141850:CDS
AAAGATGCAGGTGATATTGC+TGG 0.430331 7_4:+15446409 Msa1141850:CDS
TACTAACATTGGTTGTTTGA+AGG 0.430679 7_4:-15445144 None:intergenic
GCACCGGAAATGACCTTAAA+GGG 0.431309 7_4:-15446202 None:intergenic
CTCTTGTGTTGGAGTATGGC+AGG 0.433498 7_4:+15445550 Msa1141850:CDS
AGAGATTGAGAGAATGATCC+AGG 0.439899 7_4:+15447470 Msa1141850:CDS
GCGATCGTACTGCAGTTAAC+TGG 0.444368 7_4:+15444518 Msa1141850:five_prime_UTR
AACAATTAACATACTAACAT+TGG 0.445365 7_4:-15445155 None:intergenic
GATCAACTCTGCAATGATCA+AGG 0.458353 7_4:+15447638 Msa1141850:CDS
ATGAAAATGGAAGATTATCA+AGG 0.459072 7_4:+15447445 Msa1141850:CDS
TTTATCTTTGACCTTGGCGG+TGG 0.462714 7_4:+15446526 Msa1141850:CDS
TAACCTTGTGTCTTCTTGAA+TGG 0.468058 7_4:-15447198 None:intergenic
GAAAAGTCATGGCTTCTGAT+AGG 0.470734 7_4:+15445286 Msa1141850:five_prime_UTR
TTGTAGTTCACAAGAATTGT+CGG 0.473999 7_4:-15446235 None:intergenic
TTATGAGTGTGGTGATTCCT+AGG 0.478815 7_4:+15447163 Msa1141850:CDS
CACCCTCATAAACCTTAATT+GGG 0.479306 7_4:-15447251 None:intergenic
TGTGTTTGTGGACTTTCTGA+AGG 0.480543 7_4:+15447701 Msa1141850:CDS
TTTGATTGATGACAGCCATC+AGG 0.482837 7_4:+15447668 Msa1141850:CDS
TGATAATCCTTAGTGATTAA+GGG 0.483485 7_4:+15444026 Msa1141850:five_prime_UTR
AACAAGTAGTAGAATAGCTT+CGG 0.485003 7_4:-15444895 None:intergenic
ATTCAATACCAAGTGACAAA+GGG 0.485221 7_4:-15447128 None:intergenic
GACCTAGATGCTATATATCA+AGG 0.488303 7_4:+15446787 Msa1141850:CDS
GATGTTACCCCTTTGTCACT+TGG 0.489905 7_4:+15447120 Msa1141850:CDS
AAGCATTCCCTTAATCACTA+AGG 0.490569 7_4:-15444033 None:intergenic
GAAATTATTCACAATGATCA+AGG 0.492859 7_4:+15445590 Msa1141850:CDS
GGCACGACTTACTCTTGTGT+TGG 0.494525 7_4:+15445539 Msa1141850:CDS
GGAAGGCCACGGGGAGCAAG+AGG 0.503757 7_4:-15447327 None:intergenic
TGTGAATCATTAAAATAAGC+AGG 0.511579 7_4:-15446373 None:intergenic
TACTTTGCTATAGATGCTAA+TGG 0.512958 7_4:+15447360 Msa1141850:CDS
GAGTACAAGAAGTATTTGTT+GGG 0.513277 7_4:-15445036 None:intergenic
AATGGTACAGAAGTATTCCT+AGG 0.513462 7_4:-15447180 None:intergenic
TACTGTGAGAGGAAGGCCAC+GGG 0.519886 7_4:-15447337 None:intergenic
ACACACTAGAGAGGAGAGAA+GGG 0.524014 7_4:+15444245 Msa1141850:five_prime_UTR
AATACTAGTCACATTGAAGA+AGG 0.525318 7_4:+15445215 Msa1141850:intron
ATCATTAAAATAAGCAGGTA+CGG 0.526365 7_4:-15446368 None:intergenic
CACACACTAGAGAGGAGAGA+AGG 0.526680 7_4:+15444244 Msa1141850:five_prime_UTR
AAATAAGGTCTTTGATGTCA+AGG 0.528245 7_4:+15446579 Msa1141850:CDS
CCAGGAAGCAGACAATTTCA+AGG 0.533034 7_4:+15447488 Msa1141850:CDS
TGTCGGTTTGTCATCAGCAC+CGG 0.535167 7_4:-15446218 None:intergenic
GATGATGTTGTCCTTGTTGG+TGG 0.537032 7_4:+15446934 Msa1141850:CDS
AAATACTTCTTGTACTCACT+CGG 0.550564 7_4:+15445042 Msa1141850:five_prime_UTR
GAGAGAGAGGTCTAGGGTCG+CGG 0.552499 7_4:-15444803 None:intergenic
GTCCCAATTAAGGTTTATGA+GGG 0.553839 7_4:+15447249 Msa1141850:CDS
GTAGAATAGCTTCGGTAACC+AGG 0.560617 7_4:-15444887 None:intergenic
AATTCAATACCAAGTGACAA+AGG 0.562656 7_4:-15447129 None:intergenic
CGAAGAGCCACTAAAGATGC+AGG 0.564548 7_4:+15446397 Msa1141850:CDS
CACTAAGAATGTTCCAAACA+TGG 0.566528 7_4:+15447089 Msa1141850:CDS
GTTGCGATCGGAATTGACCT+TGG 0.566821 7_4:+15445518 Msa1141850:CDS
TACCTTGATATATAGCATCT+AGG 0.567285 7_4:-15446789 None:intergenic
TATGCAAGAGCCGCCGCAGT+CGG 0.567463 7_4:-15446460 None:intergenic
GCAAGTGAGAACAACTTGCT+TGG 0.568418 7_4:+15447285 Msa1141850:CDS
CAAAGTATACTGTGAGAGGA+AGG 0.568612 7_4:-15447344 None:intergenic
ATGCAAAGAGGTTAATTGGA+AGG 0.571352 7_4:+15446137 Msa1141850:CDS
ACTAGCTATCACATTGAAGC+AGG 0.572447 7_4:+15445264 Msa1141850:five_prime_UTR
CAATCTCTCGCATCTGTGAG+AGG 0.572867 7_4:-15446303 None:intergenic
GGCAGTTCTCAACCTCCTCA+AGG 0.573614 7_4:-15446716 None:intergenic
CGATAAAGGAGAGACACAAA+GGG 0.576825 7_4:+15444000 None:intergenic
TCACTTGGTATTGAATTGAA+GGG 0.577207 7_4:+15447135 Msa1141850:CDS
TACTTGTTAATTAATCCATG+TGG 0.587638 7_4:+15444952 Msa1141850:five_prime_UTR
AAATCAACAAAGAGAGAGAG+AGG 0.589336 7_4:-15444816 None:intergenic
CTTCACTAAGCAAAACAGCC+TGG 0.589833 7_4:-15447065 None:intergenic
TGAAAATGGAAGATTATCAA+GGG 0.589866 7_4:+15447446 Msa1141850:CDS
TCTTGTGAACTACAAGGGTG+AGG 0.592468 7_4:+15446243 Msa1141850:CDS
TTCATTCACTTGAAGCACAA+TGG 0.592831 7_4:+15445478 Msa1141850:exon
TCAGAGAGAAGAGAAGAGCA+TGG 0.595929 7_4:+15444278 Msa1141850:five_prime_UTR
TATAGCATCTAGGTCAATTG+TGG 0.597011 7_4:-15446779 None:intergenic
GGTTGAGAACTGCCTGTGAG+AGG 0.597343 7_4:+15446725 Msa1141850:CDS
TAGGTGAGTGTCTCCAGCAG+TGG 0.598292 7_4:-15446602 None:intergenic
AAATATGAAGGAGTTGCGAT+CGG 0.598814 7_4:+15445506 Msa1141850:CDS
GACAATTCTTGTGAACTACA+AGG 0.599895 7_4:+15446237 Msa1141850:CDS
CTTCTAGGATTCCTAAAGTG+AGG 0.600875 7_4:+15446959 Msa1141850:CDS
ATAGCAAAGTATACTGTGAG+AGG 0.602824 7_4:-15447348 None:intergenic
TTTGTGAAAGCAACACAAGA+AGG 0.603082 7_4:-15445626 None:intergenic
GTTGAGAACTGCCTGTGAGA+GGG 0.603407 7_4:+15446726 Msa1141850:CDS
TAAAGCATTGCAAATCAACA+AGG 0.603747 7_4:+15444194 Msa1141850:five_prime_UTR
ATCAACTCTGCAATGATCAA+GGG 0.604593 7_4:+15447639 Msa1141850:CDS
ACGATAAAGGAGAGACACAA+AGG 0.608222 7_4:+15443999 None:intergenic
TATATTAAACGTCTCTGCAG+TGG 0.608686 7_4:+15447383 Msa1141850:CDS
TATCAAAGTCTTCACCGCCT+AGG 0.609377 7_4:-15446621 None:intergenic
TGAAGACTTTGATAACAGAA+TGG 0.610392 7_4:+15446630 Msa1141850:CDS
CTTCAATGTGATAGCTAGTG+TGG 0.611658 7_4:-15445260 None:intergenic
TTGCTTTCACAAATTCCCAA+AGG 0.615627 7_4:+15445636 Msa1141850:CDS
CATCAAAAGTTCCACCGCCA+AGG 0.617660 7_4:-15446537 None:intergenic
ACTTTGTGAAGGAGTTCAAG+AGG 0.618825 7_4:+15446659 Msa1141850:CDS
CAGTAGATCATTCTGAACAA+TGG 0.620189 7_4:-15446173 None:intergenic
CACAAGAGTAAGTCGTGCCA+AGG 0.620788 7_4:-15445535 None:intergenic
ATCGTACTGCAGTTAACTGG+TGG 0.625134 7_4:+15444521 Msa1141850:five_prime_UTR
CATTGAAGCAGGAAAAGTCA+TGG 0.626556 7_4:+15445275 Msa1141850:five_prime_UTR
ACACAAAGGTATCTTCCTGA+TGG 0.628600 7_4:-15447683 None:intergenic
AAAGTTCCTCTTGCTCCCCG+TGG 0.630492 7_4:+15447321 Msa1141850:CDS
TTCAGAATGATCTACTGTTG+TGG 0.632222 7_4:+15446179 Msa1141850:CDS
TCGGTGAAATTGAATTCAAG+AGG 0.632435 7_4:+15444165 Msa1141850:five_prime_UTR
CATAAGCAACAGCCTCATCA+GGG 0.634330 7_4:-15447032 None:intergenic
GCTATGAACAATATCAACAA+AGG 0.636965 7_4:+15447747 Msa1141850:CDS
CAAGAGCATTAACCCTGATG+AGG 0.639574 7_4:+15447020 Msa1141850:CDS
ACATACTACACACACTAGAG+AGG 0.641524 7_4:+15444236 Msa1141850:five_prime_UTR
ACTGCTGGAGACACTCACCT+AGG 0.643459 7_4:+15446604 Msa1141850:CDS
AATGGTGAATCACTTTGTGA+AGG 0.643840 7_4:+15446648 Msa1141850:CDS
TTCAATACCAAGTGACAAAG+GGG 0.643889 7_4:-15447127 None:intergenic
ATACTGTGAGAGGAAGGCCA+CGG 0.644258 7_4:-15447338 None:intergenic
TCCTAGAAGAACCACCAACA+AGG 0.646014 7_4:-15446945 None:intergenic
GATAATCAATGAACCGACTG+CGG 0.651446 7_4:+15446447 Msa1141850:CDS
AATCAATGAACCGACTGCGG+CGG 0.655884 7_4:+15446450 Msa1141850:CDS
CAGAAAGTCCACAAACACAA+AGG 0.658319 7_4:-15447697 None:intergenic
GCAGGAGCAGAACGATCGTG+TGG 0.659349 7_4:+15445568 Msa1141850:CDS
GAAGGGAGATATTATGAGTG+TGG 0.661270 7_4:+15447152 Msa1141850:CDS
ACAATTCTTGTGAACTACAA+GGG 0.663306 7_4:+15446238 Msa1141850:CDS
GGAACCCAAAAGCCTTGAGG+AGG 0.669169 7_4:+15446704 Msa1141850:CDS
GATGGAACCCAAAAGCCTTG+AGG 0.671002 7_4:+15446701 Msa1141850:CDS
GTACCATTCAAGAAGACACA+AGG 0.677708 7_4:+15447195 Msa1141850:CDS
AAAGCATTGCAAATCAACAA+GGG 0.678487 7_4:+15444195 Msa1141850:five_prime_UTR
CACACTAGAGAGGAGAGAAG+GGG 0.681269 7_4:+15444246 Msa1141850:five_prime_UTR
TTGCTGGTCTCAATGTAATG+CGG 0.689400 7_4:+15446425 Msa1141850:CDS
AAAAGATTATCGTATCCACA+TGG 0.701172 7_4:-15444967 None:intergenic
GACACTTTCATACGACACTG+AGG 0.726186 7_4:+15446756 Msa1141850:CDS
ACAGATGCGAGAGATTGCAG+AGG 0.728184 7_4:+15446309 Msa1141850:CDS
TCTGCAGTGGAAGAAACCAG+TGG 0.733951 7_4:+15447396 Msa1141850:CDS
GCTGGAGACACTCACCTAGG+CGG 0.737514 7_4:+15446607 Msa1141850:CDS
TAAAGGAGAGACACAAAGGG+CGG 0.745151 7_4:+15444003 None:intergenic
ACTGTGAGAGGAAGGCCACG+GGG 0.773300 7_4:-15447336 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATTAATAGTTTTAGTAAA+GGG + chr7_4:15444711-15444730 Msa1141850:five_prime_UTR 10.0%
!! TAAATTAATCTTAATTAGTT+TGG + chr7_4:15444929-15444948 Msa1141850:five_prime_UTR 10.0%
!! TATTCTTACAATTAAAAATA+AGG + chr7_4:15446564-15446583 Msa1141850:CDS 10.0%
!! ATTAGATAAAAAGAAGAAAA+AGG + chr7_4:15444091-15444110 Msa1141850:five_prime_UTR 15.0%
!! TTAGATAAAAAGAAGAAAAA+GGG + chr7_4:15444092-15444111 Msa1141850:five_prime_UTR 15.0%
!!! ACTAAAACTATTAATTTGTT+TGG - chr7_4:15444708-15444727 None:intergenic 15.0%
!!! CAAATTAATAGTTTTAGTAA+AGG + chr7_4:15444710-15444729 Msa1141850:five_prime_UTR 15.0%
!! ATTACAATAACTAATGAAAA+TGG + chr7_4:15447432-15447451 Msa1141850:CDS 15.0%
!!! TTAAATTTGTCATCTTTTGT+TGG + chr7_4:15444316-15444335 Msa1141850:five_prime_UTR 20.0%
!!! ATCAAACAAAGTGTTAATTT+AGG + chr7_4:15444658-15444677 Msa1141850:five_prime_UTR 20.0%
!! TTAATTAGAACTAGACATAA+AGG + chr7_4:15444759-15444778 Msa1141850:five_prime_UTR 20.0%
!! AACAATTAACATACTAACAT+TGG - chr7_4:15445158-15445177 None:intergenic 20.0%
!!! TATTATAGATCACCATTTTA+GGG + chr7_4:15445818-15445837 Msa1141850:intron 20.0%
!!! TTACTTTCTAAAAACATTCT+TGG + chr7_4:15445846-15445865 Msa1141850:intron 20.0%
!!! TTCATTTTAAATTGCTAACA+TGG + chr7_4:15445871-15445890 Msa1141850:intron 20.0%
!!! TCATTTTAAATTGCTAACAT+GGG + chr7_4:15445872-15445891 Msa1141850:intron 20.0%
!!! ATTTTTGGAAGTTTTGAAAA+TGG - chr7_4:15445993-15446012 None:intergenic 20.0%
!! GTTGATATGAAAATGAAAAT+AGG + chr7_4:15446087-15446106 Msa1141850:intron 20.0%
!!! AATATTTTTATCTTTGACCT+TGG + chr7_4:15446520-15446539 Msa1141850:CDS 20.0%
! TGATAATCCTTAGTGATTAA+GGG + chr7_4:15444026-15444045 Msa1141850:five_prime_UTR 25.0%
!!! ACCTTTTTGAAACAAACAAT+TGG + chr7_4:15444056-15444075 Msa1141850:five_prime_UTR 25.0%
!! AAGAAGAAAAAGGGTTTTTA+CGG + chr7_4:15444101-15444120 Msa1141850:five_prime_UTR 25.0%
! ATCAATAGAACAAACTTGAT+TGG - chr7_4:15444465-15444484 None:intergenic 25.0%
! TGATTGGAAGACTAAAATTA+AGG - chr7_4:15444588-15444607 None:intergenic 25.0%
!! GAAAAGCATAAAGTGTAAAA+AGG - chr7_4:15444616-15444635 None:intergenic 25.0%
!!! AATAGTTTTAGTAAAGGGAT+TGG + chr7_4:15444716-15444735 Msa1141850:five_prime_UTR 25.0%
!! TACTTGTTAATTAATCCATG+TGG + chr7_4:15444952-15444971 Msa1141850:five_prime_UTR 25.0%
!!! TGTATAGTAGTATTTTGGAA+AGG - chr7_4:15445399-15445418 None:intergenic 25.0%
!! TGTAGTGTATAGTAGTATTT+TGG - chr7_4:15445404-15445423 None:intergenic 25.0%
!! TTTGAATTGGCTTCAAAATT+TGG - chr7_4:15445441-15445460 None:intergenic 25.0%
!! TTTCTAGAGAAGATTTGAAT+TGG - chr7_4:15445454-15445473 None:intergenic 25.0%
!! ATTCAAATCTTCTCTAGAAA+TGG + chr7_4:15445454-15445473 Msa1141850:five_prime_UTR 25.0%
! ACAATGGCAAAAAAATATGA+AGG + chr7_4:15445494-15445513 Msa1141850:exon 25.0%
! GAAATTATTCACAATGATCA+AGG + chr7_4:15445590-15445609 Msa1141850:CDS 25.0%
! AACTAAAAATTAAACAGTGC+TGG - chr7_4:15445742-15445761 None:intergenic 25.0%
!! GTATTATAGATCACCATTTT+AGG + chr7_4:15445817-15445836 Msa1141850:intron 25.0%
!! GGTAACATATTTGACATTTT+TGG - chr7_4:15446008-15446027 None:intergenic 25.0%
! TTTGCATCTGCAAAAATTAA+GGG - chr7_4:15446124-15446143 None:intergenic 25.0%
! ATCATTAAAATAAGCAGGTA+CGG - chr7_4:15446371-15446390 None:intergenic 25.0%
! TGTGAATCATTAAAATAAGC+AGG - chr7_4:15446376-15446395 None:intergenic 25.0%
! AGAACAAAAAGGATATTAGA+TGG + chr7_4:15446683-15446702 Msa1141850:CDS 25.0%
!!! TTATTACAGGAGTTTTTCAA+AGG + chr7_4:15446985-15447004 Msa1141850:CDS 25.0%
!!! TATTACAGGAGTTTTTCAAA+GGG + chr7_4:15446986-15447005 Msa1141850:CDS 25.0%
!!! AATTTCATTTTTATTGCCAC+TGG - chr7_4:15447415-15447434 None:intergenic 25.0%
! ATGAAAATGGAAGATTATCA+AGG + chr7_4:15447445-15447464 Msa1141850:CDS 25.0%
! TGAAAATGGAAGATTATCAA+GGG + chr7_4:15447446-15447465 Msa1141850:CDS 25.0%
GTGATAATCCTTAGTGATTA+AGG + chr7_4:15444025-15444044 Msa1141850:five_prime_UTR 30.0%
GCCAATTGTTTGTTTCAAAA+AGG - chr7_4:15444060-15444079 None:intergenic 30.0%
! ACCTTACTGTGAAGTAATTT+TGG + chr7_4:15444140-15444159 Msa1141850:five_prime_UTR 30.0%
! CCTTACTGTGAAGTAATTTT+GGG + chr7_4:15444141-15444160 Msa1141850:five_prime_UTR 30.0%
! CTTACTGTGAAGTAATTTTG+GGG + chr7_4:15444142-15444161 Msa1141850:five_prime_UTR 30.0%
!! TAAAGCATTGCAAATCAACA+AGG + chr7_4:15444194-15444213 Msa1141850:five_prime_UTR 30.0%
!! AAAGCATTGCAAATCAACAA+GGG + chr7_4:15444195-15444214 Msa1141850:five_prime_UTR 30.0%
TCTAGTCTCAATTGTCTTAT+AGG - chr7_4:15444548-15444567 None:intergenic 30.0%
GTGTAAAAAGGTTGAATGAT+TGG - chr7_4:15444604-15444623 None:intergenic 30.0%
!!! TTACTTTTTACTTGGTTACC+TGG + chr7_4:15444869-15444888 Msa1141850:five_prime_UTR 30.0%
AACAAGTAGTAGAATAGCTT+CGG - chr7_4:15444898-15444917 None:intergenic 30.0%
AAAAGATTATCGTATCCACA+TGG - chr7_4:15444970-15444989 None:intergenic 30.0%
!! GAGTACAAGAAGTATTTGTT+GGG - chr7_4:15445039-15445058 None:intergenic 30.0%
!! TGAGTACAAGAAGTATTTGT+TGG - chr7_4:15445040-15445059 None:intergenic 30.0%
AAATACTTCTTGTACTCACT+CGG + chr7_4:15445042-15445061 Msa1141850:five_prime_UTR 30.0%
! TACTAACATTGGTTGTTTGA+AGG - chr7_4:15445147-15445166 None:intergenic 30.0%
AATACTAGTCACATTGAAGA+AGG + chr7_4:15445215-15445234 Msa1141850:intron 30.0%
!! TACCAAATACAGTGTTGATT+GGG - chr7_4:15445700-15445719 None:intergenic 30.0%
!! TTACCAAATACAGTGTTGAT+TGG - chr7_4:15445701-15445720 None:intergenic 30.0%
AAAGTAAACAAGCCCTAAAA+TGG - chr7_4:15445833-15445852 None:intergenic 30.0%
CTCATAGTTAATAATTGTGC+TGG + chr7_4:15445904-15445923 Msa1141850:intron 30.0%
CTTTGCATCTGCAAAAATTA+AGG - chr7_4:15446125-15446144 None:intergenic 30.0%
! TAATTTTTGCAGATGCAAAG+AGG + chr7_4:15446125-15446144 Msa1141850:intron 30.0%
TTGTAGTTCACAAGAATTGT+CGG - chr7_4:15446238-15446257 None:intergenic 30.0%
ACAATTCTTGTGAACTACAA+GGG + chr7_4:15446238-15446257 Msa1141850:CDS 30.0%
! ATTTTTATCTTTGACCTTGG+CGG + chr7_4:15446523-15446542 Msa1141850:CDS 30.0%
AAATAAGGTCTTTGATGTCA+AGG + chr7_4:15446579-15446598 Msa1141850:CDS 30.0%
! TGAAGACTTTGATAACAGAA+TGG + chr7_4:15446630-15446649 Msa1141850:CDS 30.0%
TACCTTGATATATAGCATCT+AGG - chr7_4:15446792-15446811 None:intergenic 30.0%
! ACTTTTGTTCATCAATCACT+CGG + chr7_4:15446815-15446834 Msa1141850:CDS 30.0%
CATATTCAATTGCTCAAACT+TGG - chr7_4:15446842-15446861 None:intergenic 30.0%
CAAGTTTGAGCAATTGAATA+TGG + chr7_4:15446840-15446859 Msa1141850:CDS 30.0%
! TGTACATACACTTTTCAAAG+AGG - chr7_4:15446867-15446886 None:intergenic 30.0%
ATTCAATACCAAGTGACAAA+GGG - chr7_4:15447131-15447150 None:intergenic 30.0%
AATTCAATACCAAGTGACAA+AGG - chr7_4:15447132-15447151 None:intergenic 30.0%
! TCACTTGGTATTGAATTGAA+GGG + chr7_4:15447135-15447154 Msa1141850:CDS 30.0%
! TACTTTGCTATAGATGCTAA+TGG + chr7_4:15447360-15447379 Msa1141850:CDS 30.0%
!!! TGATCTTCACTTTTTCTACA+GGG - chr7_4:15447623-15447642 None:intergenic 30.0%
!!! TTGATCTTCACTTTTTCTAC+AGG - chr7_4:15447624-15447643 None:intergenic 30.0%
GCTATGAACAATATCAACAA+AGG + chr7_4:15447747-15447766 Msa1141850:CDS 30.0%
AAGCATTCCCTTAATCACTA+AGG - chr7_4:15444036-15444055 None:intergenic 35.0%
CCCAAAATTACTTCACAGTA+AGG - chr7_4:15444144-15444163 None:intergenic 35.0%
TCGGTGAAATTGAATTCAAG+AGG + chr7_4:15444165-15444184 Msa1141850:five_prime_UTR 35.0%
AAATCAACAAAGAGAGAGAG+AGG - chr7_4:15444819-15444838 None:intergenic 35.0%
TTCATTCACTTGAAGCACAA+TGG + chr7_4:15445478-15445497 Msa1141850:exon 35.0%
AAATATGAAGGAGTTGCGAT+CGG + chr7_4:15445506-15445525 Msa1141850:CDS 35.0%
TTTGTGAAAGCAACACAAGA+AGG - chr7_4:15445629-15445648 None:intergenic 35.0%
TTGCTTTCACAAATTCCCAA+AGG + chr7_4:15445636-15445655 Msa1141850:CDS 35.0%
AACCCAATCAACACTGTATT+TGG + chr7_4:15445695-15445714 Msa1141850:CDS 35.0%
TGGTGATCTATAATACGTTG+TGG - chr7_4:15445813-15445832 None:intergenic 35.0%
! GTCATTCGCTTCTACAATTT+TGG - chr7_4:15446029-15446048 None:intergenic 35.0%
ATGCAAAGAGGTTAATTGGA+AGG + chr7_4:15446137-15446156 Msa1141850:CDS 35.0%
CAGTAGATCATTCTGAACAA+TGG - chr7_4:15446176-15446195 None:intergenic 35.0%
TTCAGAATGATCTACTGTTG+TGG + chr7_4:15446179-15446198 Msa1141850:CDS 35.0%
GACAATTCTTGTGAACTACA+AGG + chr7_4:15446237-15446256 Msa1141850:CDS 35.0%
GTAATCACTGCATTCTTAAC+AGG - chr7_4:15446349-15446368 None:intergenic 35.0%
AATATCACCTGCATCTTTAG+TGG - chr7_4:15446407-15446426 None:intergenic 35.0%
!! CACAATTAGCTCTCTTTTGA+AGG - chr7_4:15446489-15446508 None:intergenic 35.0%
AATGGTGAATCACTTTGTGA+AGG + chr7_4:15446648-15446667 Msa1141850:CDS 35.0%
GTTCAAGAGGAAGAACAAAA+AGG + chr7_4:15446672-15446691 Msa1141850:CDS 35.0%
TATAGCATCTAGGTCAATTG+TGG - chr7_4:15446782-15446801 None:intergenic 35.0%
GACCTAGATGCTATATATCA+AGG + chr7_4:15446787-15446806 Msa1141850:CDS 35.0%
! CTTTTGTTCATCAATCACTC+GGG + chr7_4:15446816-15446835 Msa1141850:CDS 35.0%
! CTGTCTTGTTGATGCTAAAA+TGG + chr7_4:15446897-15446916 Msa1141850:CDS 35.0%
TGTAATAACTGCCTCACTTT+AGG - chr7_4:15446973-15446992 None:intergenic 35.0%
TAAAGTGAGGCAGTTATTAC+AGG + chr7_4:15446972-15446991 Msa1141850:CDS 35.0%
CACTAAGAATGTTCCAAACA+TGG + chr7_4:15447089-15447108 Msa1141850:CDS 35.0%
TTCAATACCAAGTGACAAAG+GGG - chr7_4:15447130-15447149 None:intergenic 35.0%
! GTCACTTGGTATTGAATTGA+AGG + chr7_4:15447134-15447153 Msa1141850:CDS 35.0%
!! AATGGTACAGAAGTATTCCT+AGG - chr7_4:15447183-15447202 None:intergenic 35.0%
TAACCTTGTGTCTTCTTGAA+TGG - chr7_4:15447201-15447220 None:intergenic 35.0%
AGTCCCAATTAAGGTTTATG+AGG + chr7_4:15447248-15447267 Msa1141850:CDS 35.0%
GTCCCAATTAAGGTTTATGA+GGG + chr7_4:15447249-15447268 Msa1141850:CDS 35.0%
CACCCTCATAAACCTTAATT+GGG - chr7_4:15447254-15447273 None:intergenic 35.0%
TCACCCTCATAAACCTTAAT+TGG - chr7_4:15447255-15447274 None:intergenic 35.0%
ATAGCAAAGTATACTGTGAG+AGG - chr7_4:15447351-15447370 None:intergenic 35.0%
TATATTAAACGTCTCTGCAG+TGG + chr7_4:15447383-15447402 Msa1141850:CDS 35.0%
AGTTGAAGCAAGAAATGCTT+TGG + chr7_4:15447536-15447555 Msa1141850:CDS 35.0%
!!! GATCTTCACTTTTTCTACAG+GGG - chr7_4:15447622-15447641 None:intergenic 35.0%
ATCAACTCTGCAATGATCAA+GGG + chr7_4:15447639-15447658 Msa1141850:CDS 35.0%
!!! CTGTGAAGTAATTTTGGGGT+CGG + chr7_4:15444146-15444165 Msa1141850:five_prime_UTR 40.0%
ACATACTACACACACTAGAG+AGG + chr7_4:15444236-15444255 Msa1141850:five_prime_UTR 40.0%
GTAAAAAGTAACGACGAGGT+AGG - chr7_4:15444860-15444879 None:intergenic 40.0%
CCAAGTAAAAAGTAACGACG+AGG - chr7_4:15444864-15444883 None:intergenic 40.0%
!!! CCTCGTCGTTACTTTTTACT+TGG + chr7_4:15444861-15444880 Msa1141850:five_prime_UTR 40.0%
CTTCAATGTGATAGCTAGTG+TGG - chr7_4:15445263-15445282 None:intergenic 40.0%
ACTAGCTATCACATTGAAGC+AGG + chr7_4:15445264-15445283 Msa1141850:five_prime_UTR 40.0%
CATTGAAGCAGGAAAAGTCA+TGG + chr7_4:15445275-15445294 Msa1141850:five_prime_UTR 40.0%
GAAAAGTCATGGCTTCTGAT+AGG + chr7_4:15445286-15445305 Msa1141850:five_prime_UTR 40.0%
CTTACTCTTGTGTTGGAGTA+TGG + chr7_4:15445546-15445565 Msa1141850:CDS 40.0%
ACAAATTCCCAAAGGCTGAT+TGG + chr7_4:15445644-15445663 Msa1141850:CDS 40.0%
! TGGTGATGCTGCTAAAAATC+AGG + chr7_4:15445664-15445683 Msa1141850:CDS 40.0%
GCAGATGCAAAGAGGTTAAT+TGG + chr7_4:15446133-15446152 Msa1141850:intron 40.0%
AAAGATGCAGGTGATATTGC+TGG + chr7_4:15446409-15446428 Msa1141850:CDS 40.0%
! TTGCTGGTCTCAATGTAATG+CGG + chr7_4:15446425-15446444 Msa1141850:CDS 40.0%
GATAATCAATGAACCGACTG+CGG + chr7_4:15446447-15446466 Msa1141850:CDS 40.0%
ACTTTGTGAAGGAGTTCAAG+AGG + chr7_4:15446659-15446678 Msa1141850:CDS 40.0%
!! CTTCTAGGATTCCTAAAGTG+AGG + chr7_4:15446959-15446978 Msa1141850:CDS 40.0%
!! ACAGGAGTTTTTCAAAGGGA+AGG + chr7_4:15446990-15447009 Msa1141850:CDS 40.0%
GAAGGGAGATATTATGAGTG+TGG + chr7_4:15447152-15447171 Msa1141850:CDS 40.0%
TTATGAGTGTGGTGATTCCT+AGG + chr7_4:15447163-15447182 Msa1141850:CDS 40.0%
GTACCATTCAAGAAGACACA+AGG + chr7_4:15447195-15447214 Msa1141850:CDS 40.0%
CCTTAATTGGGACTGAAGAT+TGG - chr7_4:15447242-15447261 None:intergenic 40.0%
CCAATCTTCAGTCCCAATTA+AGG + chr7_4:15447239-15447258 Msa1141850:CDS 40.0%
CAAAGTATACTGTGAGAGGA+AGG - chr7_4:15447347-15447366 None:intergenic 40.0%
AGAGATTGAGAGAATGATCC+AGG + chr7_4:15447470-15447489 Msa1141850:CDS 40.0%
GATCAACTCTGCAATGATCA+AGG + chr7_4:15447638-15447657 Msa1141850:CDS 40.0%
! TTTGATTGATGACAGCCATC+AGG + chr7_4:15447668-15447687 Msa1141850:CDS 40.0%
!! ACACAAAGGTATCTTCCTGA+TGG - chr7_4:15447686-15447705 None:intergenic 40.0%
GGAAGATACCTTTGTGTTTG+TGG + chr7_4:15447689-15447708 Msa1141850:CDS 40.0%
CAGAAAGTCCACAAACACAA+AGG - chr7_4:15447700-15447719 None:intergenic 40.0%
TGTGTTTGTGGACTTTCTGA+AGG + chr7_4:15447701-15447720 Msa1141850:CDS 40.0%
ACACACTAGAGAGGAGAGAA+GGG + chr7_4:15444245-15444264 Msa1141850:five_prime_UTR 45.0%
TCAGAGAGAAGAGAAGAGCA+TGG + chr7_4:15444278-15444297 Msa1141850:five_prime_UTR 45.0%
ATCGTACTGCAGTTAACTGG+TGG + chr7_4:15444521-15444540 Msa1141850:five_prime_UTR 45.0%
CAAAGAGAGAGAGAGGTCTA+GGG - chr7_4:15444812-15444831 None:intergenic 45.0%
ACAAAGAGAGAGAGAGGTCT+AGG - chr7_4:15444813-15444832 None:intergenic 45.0%
GTAGAATAGCTTCGGTAACC+AGG - chr7_4:15444890-15444909 None:intergenic 45.0%
AGCATCACCAATCAGCCTTT+GGG - chr7_4:15445654-15445673 None:intergenic 45.0%
TCTACTGTTGTGGCCCTTTA+AGG + chr7_4:15446189-15446208 Msa1141850:CDS 45.0%
!! TGGCCCTTTAAGGTCATTTC+CGG + chr7_4:15446199-15446218 Msa1141850:CDS 45.0%
GCACCGGAAATGACCTTAAA+GGG - chr7_4:15446205-15446224 None:intergenic 45.0%
! AGCACCGGAAATGACCTTAA+AGG - chr7_4:15446206-15446225 None:intergenic 45.0%
TCTTGTGAACTACAAGGGTG+AGG + chr7_4:15446243-15446262 Msa1141850:CDS 45.0%
!! AGAGATTGCAGAGGCATTCT+TGG + chr7_4:15446318-15446337 Msa1141850:CDS 45.0%
TTTATCTTTGACCTTGGCGG+TGG + chr7_4:15446526-15446545 Msa1141850:CDS 45.0%
TATCAAAGTCTTCACCGCCT+AGG - chr7_4:15446624-15446643 None:intergenic 45.0%
! TCAACCTCCTCAAGGCTTTT+GGG - chr7_4:15446711-15446730 None:intergenic 45.0%
GACACTTTCATACGACACTG+AGG + chr7_4:15446756-15446775 Msa1141850:CDS 45.0%
GTCGATGATGTTGTCCTTGT+TGG + chr7_4:15446931-15446950 Msa1141850:CDS 45.0%
GATGATGTTGTCCTTGTTGG+TGG + chr7_4:15446934-15446953 Msa1141850:CDS 45.0%
!! TCCTTGTTGGTGGTTCTTCT+AGG + chr7_4:15446944-15446963 Msa1141850:CDS 45.0%
TCCTAGAAGAACCACCAACA+AGG - chr7_4:15446948-15446967 None:intergenic 45.0%
!! CAAGAGCATTAACCCTGATG+AGG + chr7_4:15447020-15447039 Msa1141850:CDS 45.0%
CATAAGCAACAGCCTCATCA+GGG - chr7_4:15447035-15447054 None:intergenic 45.0%
CTTCACTAAGCAAAACAGCC+TGG - chr7_4:15447068-15447087 None:intergenic 45.0%
ACATCTCTCAGCACCATGTT+TGG - chr7_4:15447105-15447124 None:intergenic 45.0%
GATGTTACCCCTTTGTCACT+TGG + chr7_4:15447120-15447139 Msa1141850:CDS 45.0%
! GCAAGTGAGAACAACTTGCT+TGG + chr7_4:15447285-15447304 Msa1141850:CDS 45.0%
CCTTGAAATTGTCTGCTTCC+TGG - chr7_4:15447491-15447510 None:intergenic 45.0%
CCAGGAAGCAGACAATTTCA+AGG + chr7_4:15447488-15447507 Msa1141850:CDS 45.0%
CACACACTAGAGAGGAGAGA+AGG + chr7_4:15444244-15444263 Msa1141850:five_prime_UTR 50.0%
CACACTAGAGAGGAGAGAAG+GGG + chr7_4:15444246-15444265 Msa1141850:five_prime_UTR 50.0%
GCGATCGTACTGCAGTTAAC+TGG + chr7_4:15444518-15444537 Msa1141850:five_prime_UTR 50.0%
GTTGCGATCGGAATTGACCT+TGG + chr7_4:15445518-15445537 Msa1141850:CDS 50.0%
CACAAGAGTAAGTCGTGCCA+AGG - chr7_4:15445538-15445557 None:intergenic 50.0%
GGCACGACTTACTCTTGTGT+TGG + chr7_4:15445539-15445558 Msa1141850:CDS 50.0%
CTCTTGTGTTGGAGTATGGC+AGG + chr7_4:15445550-15445569 Msa1141850:CDS 50.0%
CAGCATCACCAATCAGCCTT+TGG - chr7_4:15445655-15445674 None:intergenic 50.0%
!! TGTCGGTTTGTCATCAGCAC+CGG - chr7_4:15446221-15446240 None:intergenic 50.0%
CAATCTCTCGCATCTGTGAG+AGG - chr7_4:15446306-15446325 None:intergenic 50.0%
ACAGATGCGAGAGATTGCAG+AGG + chr7_4:15446309-15446328 Msa1141850:CDS 50.0%
CGAAGAGCCACTAAAGATGC+AGG + chr7_4:15446397-15446416 Msa1141850:CDS 50.0%
AATCAATGAACCGACTGCGG+CGG + chr7_4:15446450-15446469 Msa1141850:CDS 50.0%
CATCAAAAGTTCCACCGCCA+AGG - chr7_4:15446540-15446559 None:intergenic 50.0%
TTTGATGTCAAGGCCACTGC+TGG + chr7_4:15446589-15446608 Msa1141850:CDS 50.0%
GATGGAACCCAAAAGCCTTG+AGG + chr7_4:15446701-15446720 Msa1141850:CDS 50.0%
! CTCAACCTCCTCAAGGCTTT+TGG - chr7_4:15446712-15446731 None:intergenic 50.0%
GTTGAGAACTGCCTGTGAGA+GGG + chr7_4:15446726-15446745 Msa1141850:CDS 50.0%
! TGTCCTTTTTGCCCTCTCAC+AGG - chr7_4:15446740-15446759 None:intergenic 50.0%
CCATAAGCAACAGCCTCATC+AGG - chr7_4:15447036-15447055 None:intergenic 50.0%
! CCTGATGAGGCTGTTGCTTA+TGG + chr7_4:15447033-15447052 Msa1141850:CDS 50.0%
ATACTGTGAGAGGAAGGCCA+CGG - chr7_4:15447341-15447360 None:intergenic 50.0%
TCTGCAGTGGAAGAAACCAG+TGG + chr7_4:15447396-15447415 Msa1141850:CDS 50.0%
TAGGTGAGTGTCTCCAGCAG+TGG - chr7_4:15446605-15446624 None:intergenic 55.0%
ACTGCTGGAGACACTCACCT+AGG + chr7_4:15446604-15446623 Msa1141850:CDS 55.0%
GGAACCCAAAAGCCTTGAGG+AGG + chr7_4:15446704-15446723 Msa1141850:CDS 55.0%
GGCAGTTCTCAACCTCCTCA+AGG - chr7_4:15446719-15446738 None:intergenic 55.0%
GGTTGAGAACTGCCTGTGAG+AGG + chr7_4:15446725-15446744 Msa1141850:CDS 55.0%
CTGCCTGTGAGAGGGCAAAA+AGG + chr7_4:15446734-15446753 Msa1141850:CDS 55.0%
TGCTTATGGTGCAGCTGTCC+AGG + chr7_4:15447047-15447066 Msa1141850:CDS 55.0%
AAAGTTCCTCTTGCTCCCCG+TGG + chr7_4:15447321-15447340 Msa1141850:CDS 55.0%
TACTGTGAGAGGAAGGCCAC+GGG - chr7_4:15447340-15447359 None:intergenic 55.0%
! GAGAGAGAGGTCTAGGGTCG+CGG - chr7_4:15444806-15444825 None:intergenic 60.0%
GCAGGAGCAGAACGATCGTG+TGG + chr7_4:15445568-15445587 Msa1141850:CDS 60.0%
TATGCAAGAGCCGCCGCAGT+CGG - chr7_4:15446463-15446482 None:intergenic 60.0%
ACTGCGGCGGCTCTTGCATA+TGG + chr7_4:15446463-15446482 Msa1141850:CDS 60.0%
GCTGGAGACACTCACCTAGG+CGG + chr7_4:15446607-15446626 Msa1141850:CDS 60.0%
ACTGTGAGAGGAAGGCCACG+GGG - chr7_4:15447339-15447358 None:intergenic 60.0%
GGAAGGCCACGGGGAGCAAG+AGG - chr7_4:15447330-15447349 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr7_4 gene 15444014 15447850 15444014 ID=Msa1141850;Name=Msa1141850
chr7_4 mRNA 15444014 15447850 15444014 ID=Msa1141850-mRNA-1;Parent=Msa1141850;Name=Msa1141850-mRNA-1;_AED=0.02;_eAED=0.02;_QI=1463|1|1|1|0.5|0.33|3|77|618
chr7_4 exon 15444014 15445202 15444014 ID=Msa1141850-mRNA-1:exon:3547;Parent=Msa1141850-mRNA-1
chr7_4 exon 15445223 15445716 15445223 ID=Msa1141850-mRNA-1:exon:3548;Parent=Msa1141850-mRNA-1
chr7_4 exon 15446137 15447850 15446137 ID=Msa1141850-mRNA-1:exon:3549;Parent=Msa1141850-mRNA-1
chr7_4 five_prime_UTR 15444014 15445202 15444014 ID=Msa1141850-mRNA-1:five_prime_utr;Parent=Msa1141850-mRNA-1
chr7_4 five_prime_UTR 15445223 15445496 15445223 ID=Msa1141850-mRNA-1:five_prime_utr;Parent=Msa1141850-mRNA-1
chr7_4 CDS 15445497 15445716 15445497 ID=Msa1141850-mRNA-1:cds;Parent=Msa1141850-mRNA-1
chr7_4 CDS 15446137 15447773 15446137 ID=Msa1141850-mRNA-1:cds;Parent=Msa1141850-mRNA-1
chr7_4 three_prime_UTR 15447774 15447850 15447774 ID=Msa1141850-mRNA-1:three_prime_utr;Parent=Msa1141850-mRNA-1
Gene Sequence

>Msa1141850

ATGGCAAAAAAATATGAAGGAGTTGCGATCGGAATTGACCTTGGCACGACTTACTCTTGTGTTGGAGTATGGCAGGAGCAGAACGATCGTGTGGAAATTATTCACAATGATCAAGGAAACAAAACAACACCTTCTTGTGTTGCTTTCACAAATTCCCAAAGGCTGATTGGTGATGCTGCTAAAAATCAGGCTTCTTCTAACCCAATCAACACTGTATTTGATGCAAAGAGGTTAATTGGAAGGAAATATAGTGATTCCATTGTTCAGAATGATCTACTGTTGTGGCCCTTTAAGGTCATTTCCGGTGCTGATGACAAACCGACAATTCTTGTGAACTACAAGGGTGAGGAAAAGCACTTTGTTGCTGAAGAAATATCATCTGTGATCCTCTCACAGATGCGAGAGATTGCAGAGGCATTCTTGGAGTCACCTGTTAAGAATGCAGTGATTACCGTACCTGCTTATTTTAATGATTCACAACGAAGAGCCACTAAAGATGCAGGTGATATTGCTGGTCTCAATGTAATGCGGATAATCAATGAACCGACTGCGGCGGCTCTTGCATATGGCCTTCAAAAGAGAGCTAATTGTGTTGATAAGAGAAATATTTTTATCTTTGACCTTGGCGGTGGAACTTTTGATGTGTCTATTCTTACAATTAAAAATAAGGTCTTTGATGTCAAGGCCACTGCTGGAGACACTCACCTAGGCGGTGAAGACTTTGATAACAGAATGGTGAATCACTTTGTGAAGGAGTTCAAGAGGAAGAACAAAAAGGATATTAGATGGAACCCAAAAGCCTTGAGGAGGTTGAGAACTGCCTGTGAGAGGGCAAAAAGGACACTTTCATACGACACTGAGGCCACAATTGACCTAGATGCTATATATCAAGGTATTGACTTTTGTTCATCAATCACTCGGGCCAAGTTTGAGCAATTGAATATGGACCTCTTTGAAAAGTGTATGTACACTGTTAATAGCTGTCTTGTTGATGCTAAAATGGACAAGAATAGTGTCGATGATGTTGTCCTTGTTGGTGGTTCTTCTAGGATTCCTAAAGTGAGGCAGTTATTACAGGAGTTTTTCAAAGGGAAGGAATTATGCAAGAGCATTAACCCTGATGAGGCTGTTGCTTATGGTGCAGCTGTCCAGGCTGTTTTGCTTAGTGAAGACACTAAGAATGTTCCAAACATGGTGCTGAGAGATGTTACCCCTTTGTCACTTGGTATTGAATTGAAGGGAGATATTATGAGTGTGGTGATTCCTAGGAATACTTCTGTACCATTCAAGAAGACACAAGGTTATTCAACAGCTTCAGATTACCAATCTTCAGTCCCAATTAAGGTTTATGAGGGTGAGAGAATTGTTGCAAGTGAGAACAACTTGCTTGGTTTGTTTAAGCTCAAAGTTCCTCTTGCTCCCCGTGGCCTTCCTCTCACAGTATACTTTGCTATAGATGCTAATGGTATATTAAACGTCTCTGCAGTGGAAGAAACCAGTGGCAATAAAAATGAAATTACAATAACTAATGAAAATGGAAGATTATCAAGGGAAGAGATTGAGAGAATGATCCAGGAAGCAGACAATTTCAAGGCTCAAGACATGAAGTTCAAAAAGAAAGTTGAAGCAAGAAATGCTTTGGATGATTATCTTTACAAAGTAAGAAAAGTAATGAAGAACGATAGTGTTAGCTCAAAGCTCACCCCTGTAGAAAAAGTGAAGATCAACTCTGCAATGATCAAGGGAAAAAGTTTGATTGATGACAGCCATCAGGAAGATACCTTTGTGTTTGTGGACTTTCTGAAGGAGCTTGAGAGCATCTTTGAATCTGCTATGAACAATATCAACAAAGGTTAA
Protein sequence

>Msa1141850

MAKKYEGVAIGIDLGTTYSCVGVWQEQNDRVEIIHNDQGNKTTPSCVAFTNSQRLIGDAAKNQASSNPINTVFDAKRLIGRKYSDSIVQNDLLLWPFKVISGADDKPTILVNYKGEEKHFVAEEISSVILSQMREIAEAFLESPVKNAVITVPAYFNDSQRRATKDAGDIAGLNVMRIINEPTAAALAYGLQKRANCVDKRNIFIFDLGGGTFDVSILTIKNKVFDVKATAGDTHLGGEDFDNRMVNHFVKEFKRKNKKDIRWNPKALRRLRTACERAKRTLSYDTEATIDLDAIYQGIDFCSSITRAKFEQLNMDLFEKCMYTVNSCLVDAKMDKNSVDDVVLVGGSSRIPKVRQLLQEFFKGKELCKSINPDEAVAYGAAVQAVLLSEDTKNVPNMVLRDVTPLSLGIELKGDIMSVVIPRNTSVPFKKTQGYSTASDYQSSVPIKVYEGERIVASENNLLGLFKLKVPLAPRGLPLTVYFAIDANGILNVSAVEETSGNKNEITITNENGRLSREEIERMIQEADNFKAQDMKFKKKVEARNALDDYLYKVRKVMKNDSVSSKLTPVEKVKINSAMIKGKSLIDDSHQEDTFVFVDFLKELESIFESAMNNINKG*