AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1141950

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1141950 MtrunA17_Chr7g0223711 81.260 603 88 3 1 578 1 603 0.0 990
Msa1141950 MtrunA17_Chr8g0350731 79.402 602 100 2 1 578 29 630 0.0 971
Msa1141950 MtrunA17_Chr7g0223631 79.734 602 84 4 1 578 1 588 0.0 955
Msa1141950 MtrunA17_Chr7g0223621 75.581 602 123 2 1 578 1 602 0.0 928
Msa1141950 MtrunA17_Chr7g0223751 73.920 602 133 2 1 578 1 602 0.0 914
Msa1141950 MtrunA17_Chr7g0223701 74.468 611 123 5 1 578 1 611 0.0 905
Msa1141950 MtrunA17_Chr7g0223731 71.761 602 146 2 1 578 1 602 0.0 885
Msa1141950 MtrunA17_Chr7g0223741 71.524 597 145 4 1 572 1 597 0.0 876
Msa1141950 MtrunA17_Chr7g0223031 70.930 602 151 2 1 578 1 602 0.0 875
Msa1141950 MtrunA17_Chr7g0248131 71.005 607 147 7 1 578 1 607 0.0 854
Msa1141950 MtrunA17_Chr7g0248151 71.405 605 146 4 1 578 1 605 0.0 846
Msa1141950 MtrunA17_Chr7g0223681 82.227 512 67 2 91 578 2 513 0.0 835
Msa1141950 MtrunA17_Chr2g0293581 64.909 607 185 6 1 579 1 607 0.0 788
Msa1141950 MtrunA17_Chr7g0223781 65.000 600 187 4 1 577 1 600 0.0 787
Msa1141950 MtrunA17_Chr4g0031321 63.802 605 190 7 1 578 1 603 0.0 778
Msa1141950 MtrunA17_Chr7g0223541 71.645 529 125 4 74 577 31 559 0.0 777
Msa1141950 MtrunA17_Chr4g0031301 63.636 605 191 7 1 578 1 603 0.0 777
Msa1141950 MtrunA17_Chr2g0298851 63.062 601 197 2 1 576 1 601 0.0 776
Msa1141950 MtrunA17_Chr7g0223181 63.802 605 190 7 1 578 1 603 0.0 775
Msa1141950 MtrunA17_Chr7g0221661 63.682 603 194 3 1 578 1 603 0.0 774
Msa1141950 MtrunA17_Chr7g0262821 63.802 605 190 7 1 578 1 603 0.0 773
Msa1141950 MtrunA17_Chr7g0223291 63.306 605 193 7 1 578 1 603 0.0 771
Msa1141950 MtrunA17_Chr5g0427901 62.866 614 192 4 1 578 1 614 0.0 766
Msa1141950 MtrunA17_Chr7g0223801 62.791 602 199 4 1 578 1 601 0.0 765
Msa1141950 MtrunA17_Chr7g0233311 62.583 604 199 4 1 577 1 604 0.0 763
Msa1141950 MtrunA17_Chr2g0298861 61.039 616 202 5 1 578 1 616 0.0 759
Msa1141950 MtrunA17_Chr7g0223721 62.086 604 203 4 1 578 1 604 0.0 757
Msa1141950 MtrunA17_Chr4g0073741 61.960 602 199 8 1 575 1 599 0.0 750
Msa1141950 MtrunA17_Chr4g0040931 61.258 604 208 4 1 578 1 604 0.0 744
Msa1141950 MtrunA17_Chr7g0223851 61.383 593 204 2 1 568 10 602 0.0 737
Msa1141950 MtrunA17_Chr7g0233301 62.721 566 200 3 1 563 1 558 0.0 729
Msa1141950 MtrunA17_Chr7g0217971 60.877 593 184 3 1 569 1 569 0.0 728
Msa1141950 MtrunA17_Chr2g0287631 62.458 594 197 4 1 568 1 594 0.0 728
Msa1141950 MtrunA17_Chr7g0225671 61.421 591 200 4 3 568 12 599 0.0 725
Msa1141950 MtrunA17_Chr7g0223821 61.551 593 200 4 1 566 1 592 0.0 717
Msa1141950 MtrunA17_Chr2g0296391 65.909 528 155 3 1 503 1 528 0.0 710
Msa1141950 MtrunA17_Chr4g0012541 57.841 593 216 3 1 568 1 584 0.0 680
Msa1141950 MtrunA17_Chr8g0390821 55.640 656 198 10 1 578 1 641 0.0 675
Msa1141950 MtrunA17_Chr2g0293601 66.667 495 152 4 1 493 1 484 0.0 674
Msa1141950 MtrunA17_Chr7g0223831 57.426 606 200 7 1 578 1 576 0.0 672
Msa1141950 MtrunA17_Chr7g0215261 57.261 606 196 7 1 578 1 571 0.0 669
Msa1141950 MtrunA17_Chr6g0462601 56.250 608 235 7 1 579 1 606 0.0 661
Msa1141950 MtrunA17_Chr6g0462681 57.785 578 216 6 1 552 1 576 0.0 653
Msa1141950 MtrunA17_Chr7g0228281 56.766 569 224 4 1 568 62 609 0.0 645
Msa1141950 MtrunA17_Chr6g0462711 56.920 578 215 6 8 553 9 584 0.0 632
Msa1141950 MtrunA17_Chr6g0462771 57.192 577 214 7 8 553 8 582 0.0 631
Msa1141950 MtrunA17_Chr6g0462641 55.043 585 228 6 1 553 1 582 0.0 618
Msa1141950 MtrunA17_Chr2g0331381 59.029 515 173 4 9 523 6 482 0.0 615
Msa1141950 MtrunA17_Chr2g0284681 55.216 556 215 8 9 532 8 561 0.0 599
Msa1141950 MtrunA17_Chr2g0299821 57.795 526 169 7 1 515 1 484 0.0 597
Msa1141950 MtrunA17_Chr2g0293561 72.995 374 101 0 1 374 1 374 0.0 597
Msa1141950 MtrunA17_Chr8g0388671 50.934 589 254 9 7 566 36 618 0.0 573
Msa1141950 MtrunA17_Chr1g0166551 53.229 573 177 6 9 578 5 489 0.0 573
Msa1141950 MtrunA17_Chr8g0388761 50.594 589 256 9 7 566 36 618 0.0 573
Msa1141950 MtrunA17_Chr2g0299921 69.367 395 117 2 2 396 1022 1412 0.0 568
Msa1141950 MtrunA17_Chr2g0299921 67.733 375 116 3 2 375 1406 1776 1.00e-169 526
Msa1141950 MtrunA17_Chr2g0299851 66.923 390 125 2 1 390 1030 1415 0.0 550
Msa1141950 MtrunA17_Chr2g0299791 68.123 389 120 2 1 389 1034 1418 0.0 549
Msa1141950 MtrunA17_Chr6g0462611 54.743 506 205 3 74 555 22 527 0.0 544
Msa1141950 MtrunA17_Chr2g0293271 60.143 419 110 4 21 434 43 409 4.78e-170 489
Msa1141950 MtrunA17_Chr2g0299881 65.306 392 128 4 2 389 1031 1418 8.92e-170 520
Msa1141950 MtrunA17_Chr2g0299881 58.209 67 28 0 184 250 1409 1475 1.14e-17 87.4
Msa1141950 MtrunA17_Chr6g0462861 46.312 583 265 11 1 552 1 566 1.07e-154 458
Msa1141950 MtrunA17_Chr1g0151991 71.478 291 83 0 1 291 1 291 9.15e-153 440
Msa1141950 MtrunA17_Chr7g0245521 73.626 273 72 0 1 273 1 273 1.40e-151 436
Msa1141950 MtrunA17_Chr2g0279901 43.964 555 270 12 10 537 57 597 1.60e-144 433
Msa1141950 MtrunA17_Chr4g0049501 43.063 555 276 11 10 537 54 595 6.55e-142 426
Msa1141950 MtrunA17_Chr2g0277021 40.956 586 306 13 10 568 72 644 4.16e-133 404
Msa1141950 MtrunA17_Chr7g0223671 84.865 185 28 0 74 258 8 192 1.57e-114 339
Msa1141950 MtrunA17_Chr1g0188601 75.393 191 47 0 92 282 1 191 3.75e-104 312
Msa1141950 MtrunA17_Chr7g0245531 52.843 299 116 4 305 578 2 300 7.45e-100 305
Msa1141950 MtrunA17_Chr7g0223761 48.094 341 156 6 241 578 3 325 1.21e-95 296
Msa1141950 MtrunA17_Chr6g0462631 50.331 302 102 4 91 389 13 269 8.67e-93 286
Msa1141950 MtrunA17_Chr6g0462751 46.462 325 115 3 67 389 12 279 1.06e-89 279
Msa1141950 MtrunA17_Chr1g0152001 48.789 289 122 4 315 578 1 288 3.73e-82 260
Msa1141950 MtrunA17_Chr7g0223771 73.856 153 40 0 93 245 1 153 3.89e-79 246
Msa1141950 MtrunA17_Chr5g0394391 33.598 503 285 12 8 470 28 521 4.07e-78 257
Msa1141950 MtrunA17_Chr6g0462791 66.292 178 59 1 109 286 2 178 5.15e-78 244
Msa1141950 MtrunA17_Chr2g0331901 34.537 443 276 6 10 444 4 440 7.85e-75 254
Msa1141950 MtrunA17_Chr4g0004331 36.272 397 248 4 10 405 4 396 6.86e-74 252
Msa1141950 MtrunA17_Chr4g0046881 75.862 145 32 1 131 275 10 151 3.12e-71 225
Msa1141950 MtrunA17_Chr5g0444001 32.829 463 297 8 10 462 4 462 4.80e-68 230
Msa1141950 MtrunA17_Chr1g0188611 48.399 281 112 4 322 578 1 272 2.01e-64 213
Msa1141950 MtrunA17_Chr3g0098761 33.766 385 251 3 10 393 4 385 3.31e-64 224
Msa1141950 MtrunA17_Chr7g0223861 73.282 131 35 0 78 208 4 134 1.52e-63 204
Msa1141950 MtrunA17_Chr4g0064621 31.478 521 299 15 8 479 139 650 7.23e-60 211
Msa1141950 MtrunA17_Chr6g0462701 54.444 180 58 1 91 270 13 168 1.44e-56 187
Msa1141950 MtrunA17_Chr3g0120101 31.367 373 238 5 36 393 55 424 8.66e-55 199
Msa1141950 MtrunA17_Chr4g0060241 28.514 498 297 16 8 465 19 497 2.73e-49 179
Msa1141950 MtrunA17_Chr5g0394221 36.458 288 156 6 291 555 61 344 6.75e-48 171
Msa1141950 MtrunA17_Chr6g0481391 64.754 122 40 1 38 159 13 131 7.52e-48 163
Msa1141950 MtrunA17_Chr7g0245541 47.778 180 92 2 401 578 8 187 6.51e-44 155
Msa1141950 MtrunA17_Chr5g0442171 32.733 333 173 11 170 464 1 320 2.39e-43 157
Msa1141950 MtrunA17_Chr5g0394231 36.638 232 94 5 74 304 5 184 8.06e-42 149
Msa1141950 MtrunA17_Chr7g0223691 86.301 73 10 0 1 73 1 73 7.74e-40 139
Msa1141950 MtrunA17_Chr1g0188591 75.342 73 18 0 1 73 1 73 3.40e-31 116
Msa1141950 MtrunA17_Chr6g0481381 72.973 74 20 0 176 249 1 74 7.63e-31 119
Msa1141950 MtrunA17_Chr6g0462621 75.342 73 16 1 1 73 1 71 1.04e-29 111
Msa1141950 MtrunA17_Chr7g0223791 70.732 82 16 3 56 137 18 91 1.69e-28 112
Msa1141950 MtrunA17_Chr7g0223791 33.588 131 73 4 417 544 58 177 2.09e-14 71.6
Msa1141950 MtrunA17_Chr4g0046871 80.328 61 12 0 336 396 6 66 9.05e-27 104
Msa1141950 MtrunA17_Chr6g0462691 41.549 142 59 3 322 439 1 142 4.71e-25 101
Msa1141950 MtrunA17_Chr7g0221651 51.190 84 41 0 215 298 1 84 1.48e-23 95.1
Msa1141950 MtrunA17_Chr7g0223881 53.012 83 38 1 281 363 2 83 2.48e-22 91.3
Msa1141950 MtrunA17_Chr6g0462801 54.545 66 25 1 322 382 1 66 1.00e-15 72.4
Msa1141950 MtrunA17_Chr2g0299891 72.093 43 12 0 315 357 1 43 4.27e-15 70.5
Msa1141950 MtrunA17_Chr5g0442161 29.936 157 103 4 5 157 24 177 4.97e-13 67.8
Msa1141950 MtrunA17_Chr7g0223871 37.864 103 63 1 467 568 7 109 5.95e-13 66.6
Msa1141950 MtrunA17_Chr4g0064651 41.096 73 41 2 395 465 50 122 3.91e-12 63.5
Msa1141950 MtrunA17_Chr2g0284661 50.820 61 28 1 225 283 5 65 1.34e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1141950 AT3G12580.1 64.628 605 185 7 1 578 1 603 0.0 785
Msa1141950 AT5G02500.1 62.975 605 195 7 1 578 1 603 0.0 771
Msa1141950 AT5G02490.1 62.645 605 197 7 1 578 1 603 0.0 766
Msa1141950 AT3G09440.1 62.645 605 197 7 1 578 1 603 0.0 759
Msa1141950 AT3G09440.4 62.645 605 197 7 1 578 1 603 0.0 759
Msa1141950 AT3G09440.2 62.645 605 197 7 1 578 1 603 0.0 759
Msa1141950 AT3G09440.3 62.645 605 197 7 1 578 1 603 0.0 759
Msa1141950 AT1G56410.1 62.270 599 197 6 1 573 1 596 0.0 750
Msa1141950 AT1G16030.1 61.720 593 197 8 1 566 1 590 0.0 739
Msa1141950 AT5G02500.2 74.036 389 99 1 1 389 1 387 0.0 615
Msa1141950 AT5G02500.2 39.773 88 52 1 492 578 386 473 8.17e-11 65.1
Msa1141950 AT5G28540.1 51.952 589 248 9 7 566 35 617 0.0 586
Msa1141950 AT5G42020.1 51.783 589 249 9 7 566 35 617 0.0 583
Msa1141950 AT5G42020.3 51.783 589 249 9 7 566 40 622 0.0 582
Msa1141950 AT1G09080.2 52.603 557 232 7 7 537 40 590 0.0 558
Msa1141950 AT1G09080.1 52.603 557 232 7 7 537 50 600 0.0 557
Msa1141950 AT5G42020.2 46.769 588 225 10 7 566 35 562 5.93e-170 496
Msa1141950 AT4G37910.1 43.604 555 273 10 10 537 55 596 6.82e-143 429
Msa1141950 AT4G37910.2 43.604 555 273 10 10 537 55 596 6.82e-143 429
Msa1141950 AT5G09590.1 43.063 555 276 11 10 537 60 601 1.66e-140 423
Msa1141950 AT4G24280.1 41.227 587 304 14 10 568 81 654 4.31e-131 400
Msa1141950 AT5G49910.1 40.716 587 307 14 10 568 81 654 2.02e-129 395
Msa1141950 AT1G11660.4 29.375 640 368 12 10 572 4 636 8.80e-77 259
Msa1141950 AT1G11660.3 29.375 640 368 12 10 572 4 636 8.80e-77 259
Msa1141950 AT1G11660.1 29.375 640 368 12 10 572 4 636 8.80e-77 259
Msa1141950 AT1G79930.2 36.410 390 244 3 10 398 4 390 5.80e-76 257
Msa1141950 AT1G79930.1 36.410 390 244 3 10 398 4 390 1.66e-75 256
Msa1141950 AT1G79920.1 36.480 392 241 4 10 398 4 390 4.17e-75 253
Msa1141950 AT1G79920.3 36.480 392 241 4 10 398 4 390 4.72e-75 253
Msa1141950 AT1G79920.2 36.480 392 241 4 10 398 4 390 4.72e-75 253
Msa1141950 AT1G79920.4 36.480 392 241 4 10 398 4 390 4.72e-75 253
Msa1141950 AT1G11660.2 29.851 603 341 11 47 572 2 599 4.10e-71 243
Msa1141950 AT2G32120.1 31.189 513 299 13 8 483 29 524 9.99e-67 227
Msa1141950 AT2G32120.2 31.189 513 299 13 8 483 29 524 9.99e-67 227
Msa1141950 AT4G16660.1 28.889 405 267 6 6 393 23 423 2.10e-54 198
Msa1141950 AT4G16660.2 28.889 405 267 6 6 393 47 447 3.45e-54 198

Find 146 sgRNAs with CRISPR-Local

Find 238 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCTTACTGTGAAGTAATTT+TGG 0.095272 7_4:+15693845 Msa1141950:five_prime_UTR
CTCAACCTCCTCAAGGCTTT+TGG 0.126998 7_4:-15696208 None:intergenic
TCTACTGTTGTGGCCCTTTA+AGG 0.152881 7_4:+15695688 Msa1141950:CDS
TGTAGAGTATTGTAGTATTT+TGG 0.180452 7_4:-15695130 None:intergenic
TTTGAATTGACTTCAAAATT+TGG 0.211928 7_4:-15695167 None:intergenic
TCCTTGTTGGTGGTTCTTCT+AGG 0.229614 7_4:+15696443 Msa1141950:CDS
GCCAATTGTTTGTTTCAAAA+AGG 0.243615 7_4:-15693760 None:intergenic
GCAGATGCAAAGAGGTTAAT+TGG 0.250254 7_4:+15695632 Msa1141950:intron
TGCTTACGGTGCAGCTATTC+AGG 0.270565 7_4:+15696546 Msa1141950:CDS
AACCCAACCAACACTGTCTT+TGG 0.279913 7_4:+15695424 Msa1141950:CDS
CCTTGAAATTGTCAGCTTCC+TGG 0.285031 7_4:-15696992 None:intergenic
ATTACAATAACTAATGAAAA+TGG 0.292577 7_4:+15696936 Msa1141950:CDS
GATCAACTCTAAAATGATAA+AGG 0.309834 7_4:+15697139 Msa1141950:CDS
TGTAATAGCCGTCTCACTTT+AGG 0.328128 7_4:-15696469 None:intergenic
CTTACTCGTGTGTTGGAGTA+TGG 0.335848 7_4:+15695275 Msa1141950:CDS
CAAGTTTGAGCAATTGAATA+TGG 0.349032 7_4:+15696339 Msa1141950:CDS
AGAGTGTTCCATTCCAATCT+TGG 0.353961 7_4:+15696593 Msa1141950:CDS
GTAATCACTGCATTCTTAAC+AGG 0.359063 7_4:-15695845 None:intergenic
CAGCATCACCAATCAGCCTT+TGG 0.360159 7_4:-15695381 None:intergenic
TAAAGTGAGACGGCTATTAC+AGG 0.363248 7_4:+15696471 Msa1141950:CDS
AGCACCGGATATGACCTTAA+AGG 0.369858 7_4:-15695702 None:intergenic
CTGCCTGTGAGAGGGCAAAA+AGG 0.378093 7_4:+15696233 Msa1141950:CDS
GTGATGTAGTTTAGCGGATT+GGG 0.378983 7_4:-15693618 None:intergenic
AGTTGAAGCAAGAAATGCTT+TGG 0.381737 7_4:+15697040 Msa1141950:CDS
TGTCTTGATTAAAGTTTATG+AGG 0.387899 7_4:+15696752 Msa1141950:CDS
CCGTAAGCAACAGCCTCATC+AGG 0.392319 7_4:-15696532 None:intergenic
CCCAAAATTACTTCACAGTA+AGG 0.393135 7_4:-15693846 None:intergenic
AATATCCCCTGCATCTTTAG+TGG 0.399143 7_4:-15695903 None:intergenic
GTAGATGATGTTGTCCTTGT+TGG 0.401743 7_4:+15696430 Msa1141950:CDS
AAGAACAAAGAGGATATTAG+TGG 0.402789 7_4:+15696181 Msa1141950:CDS
GTGATAATCCTTGGTGACTA+AGG 0.408315 7_4:+15693727 Msa1141950:five_prime_UTR
AGCATCACCAATCAGCCTTT+GGG 0.410141 7_4:-15695380 None:intergenic
TACCAAAGACAGTGTTGGTT+GGG 0.410143 7_4:-15695426 None:intergenic
ACTAATTCCCAAAGGCTGAT+TGG 0.414254 7_4:+15695373 Msa1141950:CDS
ACATCTCGCAAAACCAAGAT+TGG 0.424514 7_4:-15696606 None:intergenic
CATATTCAATTGCTCAAACT+TGG 0.426433 7_4:-15696338 None:intergenic
TTTGATGTCAAGGCCACTGC+TGG 0.427311 7_4:+15696088 Msa1141950:CDS
GCACCGGATATGACCTTAAA+GGG 0.434687 7_4:-15695701 None:intergenic
AGAGATTGAGAGAATGATCC+AGG 0.439899 7_4:+15696974 Msa1141950:CDS
ATCAACTCTAAAATGATAAA+GGG 0.440128 7_4:+15697140 Msa1141950:CDS
AAAGATGCAGGGGATATTGC+TGG 0.441280 7_4:+15695908 Msa1141950:CDS
ATTCAAATCTTCTATACAAA+CGG 0.443185 7_4:+15695183 Msa1141950:five_prime_UTR
TTTGATTGATGGCAACCAAC+AGG 0.450465 7_4:+15697169 Msa1141950:CDS
GGAAGATGCATTTGTGTTTG+TGG 0.453953 7_4:+15697190 Msa1141950:CDS
TTGCTGATGCTAAGATGGAC+AGG 0.455472 7_4:+15696401 Msa1141950:CDS
TATTCTTTAGTGCTCTTGAC+AGG 0.455961 7_4:-15696702 None:intergenic
ATGAAAATGGAAGATTATCA+AGG 0.459072 7_4:+15696949 Msa1141950:CDS
AGGAAGGCCTCGAGGTGCAC+GGG 0.461960 7_4:-15696832 None:intergenic
TTTATCTTTGACCTTGGCGG+TGG 0.462714 7_4:+15696025 Msa1141950:CDS
CTTTAATCAAGACAGTGCTT+TGG 0.463007 7_4:-15696743 None:intergenic
TGTGATGTAGTTTAGCGGAT+TGG 0.469341 7_4:-15693619 None:intergenic
GGACCTCTTTGAAAAGTGTA+TGG 0.471350 7_4:+15696360 Msa1141950:CDS
TTGTAGTTCACAAGAATTGT+CGG 0.473999 7_4:-15695734 None:intergenic
TGGCCCTTTAAGGTCATATC+CGG 0.475690 7_4:+15695698 Msa1141950:CDS
CCTGATGAGGCTGTTGCTTA+CGG 0.484166 7_4:+15696532 Msa1141950:CDS
GACCTAGATGCTATATATCA+AGG 0.488303 7_4:+15696286 Msa1141950:CDS
CGAAGAGCCACTAAAGATGC+AGG 0.492827 7_4:+15695896 Msa1141950:CDS
GAAATTATTCACAATGATCA+AGG 0.492859 7_4:+15695319 Msa1141950:CDS
CCAGGAAGCTGACAATTTCA+AGG 0.494121 7_4:+15696992 Msa1141950:CDS
GATGATGTTGTCCTTGTTGG+TGG 0.496358 7_4:+15696433 Msa1141950:CDS
GCTAGTGAGAACAACCTGTT+GGG 0.498732 7_4:+15696789 Msa1141950:CDS
TTGTCTTGCTGATGCTAAGA+TGG 0.502095 7_4:+15696396 Msa1141950:CDS
TCTCATGTGATGTAGTTTAG+CGG 0.502286 7_4:-15693624 None:intergenic
CAAAGGGCGGTGATAATCCT+TGG 0.504521 7_4:+15693718 Msa1141950:five_prime_UTR
TGTGAATCATTAAAATAAGC+AGG 0.511579 7_4:-15695872 None:intergenic
TTTAGTGCTCTTGACAGGAA+TGG 0.514263 7_4:-15696697 None:intergenic
TAAAACATACTTTGTGAGGA+AGG 0.516764 7_4:-15696848 None:intergenic
GGCACGACTTACTCGTGTGT+TGG 0.523542 7_4:+15695268 Msa1141950:CDS
ACACACTAGAGAGGAGAGAA+GGG 0.524014 7_4:+15693950 Msa1141950:five_prime_UTR
CACACACTAGAGAGGAGAGA+AGG 0.526680 7_4:+15693949 Msa1141950:five_prime_UTR
ACATCACATGAGAAGAGAAA+AGG 0.527406 7_4:+15693634 Msa1141950:five_prime_UTR
AAATAAGGTCTTTGATGTCA+AGG 0.528245 7_4:+15696078 Msa1141950:CDS
ATCCTTGGTGACTAAGGGAA+TGG 0.529196 7_4:+15693733 Msa1141950:five_prime_UTR
GAGCATGGTGTAGAGATTGA+AGG 0.530467 7_4:+15693996 Msa1141950:five_prime_UTR
TGTCGGTTTGTCATCAGCAC+CGG 0.539904 7_4:-15695717 None:intergenic
GAGGAAGGCCTCGAGGTGCA+CGG 0.549827 7_4:-15696833 None:intergenic
TATGCAAGAGCCGCTGCAGT+CGG 0.550536 7_4:-15695959 None:intergenic
AGCTAGTGAGAACAACCTGT+TGG 0.556225 7_4:+15696788 Msa1141950:CDS
GTCTTGATTAAAGTTTATGA+GGG 0.561142 7_4:+15696753 Msa1141950:CDS
TGATAATCCTTGGTGACTAA+GGG 0.561232 7_4:+15693728 Msa1141950:five_prime_UTR
TCTTGCATAATTCCTTCCCC+TGG 0.565093 7_4:-15696501 None:intergenic
ACTGCAGCGGCTCTTGCATA+CGG 0.566971 7_4:+15695962 Msa1141950:CDS
TACCTTGATATATAGCATCT+AGG 0.567285 7_4:-15696288 None:intergenic
ATGCAAAGAGGTTAATTGGA+AGG 0.571352 7_4:+15695636 Msa1141950:CDS
GTTGCGATCGGAATTGACCT+TGG 0.571381 7_4:+15695247 Msa1141950:CDS
CAATCTCTCGCATCTGTGAG+AGG 0.572867 7_4:-15695802 None:intergenic
GGCAGTTCTCAACCTCCTCA+AGG 0.573614 7_4:-15696215 None:intergenic
ATTGTAAAACATACTTTGTG+AGG 0.576392 7_4:-15696852 None:intergenic
TAAATTACCAAAGACAGTGT+TGG 0.578436 7_4:-15695431 None:intergenic
AAATATGAGGGAGTTGCGAT+CGG 0.580136 7_4:+15695235 Msa1141950:CDS
ACACAAATGCATCTTCCTGT+TGG 0.581155 7_4:-15697184 None:intergenic
TTACCAAAGACAGTGTTGGT+TGG 0.584440 7_4:-15695427 None:intergenic
TCGCAAAACCAAGATTGGAA+TGG 0.584996 7_4:-15696601 None:intergenic
TGAAAATGGAAGATTATCAA+GGG 0.589866 7_4:+15696950 Msa1141950:CDS
TCTTGTGAACTACAAGGGTG+AGG 0.592468 7_4:+15695742 Msa1141950:CDS
TTGCTTTCACTAATTCCCAA+AGG 0.594534 7_4:+15695365 Msa1141950:CDS
TATAGCATCTAGGTCAATTG+TGG 0.597011 7_4:-15696278 None:intergenic
GGTTGAGAACTGCCTGTGAG+AGG 0.597343 7_4:+15696224 Msa1141950:CDS
TAGGTGAGTGTCTCCAGCAG+TGG 0.598292 7_4:-15696101 None:intergenic
GACAATTCTTGTGAACTACA+AGG 0.599895 7_4:+15695736 Msa1141950:CDS
GTTGAGAACTGCCTGTGAGA+GGG 0.603407 7_4:+15696225 Msa1141950:CDS
TAAAGCATTGCAAATCAACA+AGG 0.603747 7_4:+15693899 Msa1141950:five_prime_UTR
CTAGGATTCCTAAAGTGAGA+CGG 0.604380 7_4:+15696461 Msa1141950:CDS
ATACGGCAAAGGAGAGACAA+AGG 0.606025 7_4:+15693701 Msa1141950:five_prime_UTR
TACGGCAAAGGAGAGACAAA+GGG 0.607060 7_4:+15693702 Msa1141950:five_prime_UTR
TATCAAAGTCTTCACCGCCT+AGG 0.609377 7_4:-15696120 None:intergenic
AACCATTCCCTTAGTCACCA+AGG 0.609583 7_4:-15693735 None:intergenic
TGAAGACTTTGATAACAGAA+TGG 0.610392 7_4:+15696129 Msa1141950:CDS
TTCATTCACTTGAAGCACGA+TGG 0.611483 7_4:+15695207 Msa1141950:exon
TGCTGATGCTAAGATGGACA+GGG 0.617353 7_4:+15696402 Msa1141950:CDS
CATCAAAAGTTCCACCGCCA+AGG 0.617660 7_4:-15696036 None:intergenic
ACTTTGTGAAGGAGTTCAAG+AGG 0.618825 7_4:+15696158 Msa1141950:CDS
CAGTAGATCATTCTGAACAA+TGG 0.620189 7_4:-15695672 None:intergenic
TCAGTTCCCCGTGCACCTCG+AGG 0.621817 7_4:+15696825 Msa1141950:CDS
CGTAAGCAACAGCCTCATCA+GGG 0.624820 7_4:-15696531 None:intergenic
TTAGTGAAAGCAACACAAGA+AGG 0.625764 7_4:-15695355 None:intergenic
GAAGAGCCACTAAAGATGCA+GGG 0.626519 7_4:+15695897 Msa1141950:CDS
TTCAGAATGATCTACTGTTG+TGG 0.632222 7_4:+15695678 Msa1141950:CDS
TCGGTGAAATTGAATTCAAG+AGG 0.632435 7_4:+15693870 Msa1141950:five_prime_UTR
AGAACAAAGAGGATATTAGT+GGG 0.633089 7_4:+15696182 Msa1141950:CDS
CAAGAGCATTAACCCTGATG+AGG 0.639574 7_4:+15696519 Msa1141950:CDS
GGAAGGCCTCGAGGTGCACG+GGG 0.642607 7_4:-15696831 None:intergenic
ACTGCTGGAGACACTCACCT+AGG 0.643459 7_4:+15696103 Msa1141950:CDS
AATGGTGAATCACTTTGTGA+AGG 0.643840 7_4:+15696147 Msa1141950:CDS
GTTCAAGAGGAAGAACAAAG+AGG 0.645922 7_4:+15696171 Msa1141950:CDS
TCCTAGAAGAACCACCAACA+AGG 0.646014 7_4:-15696444 None:intergenic
AATCAATGAACCGACTGCAG+CGG 0.648971 7_4:+15695949 Msa1141950:CDS
ACACACTACACACACTAGAG+AGG 0.653071 7_4:+15693941 Msa1141950:five_prime_UTR
CACACGAGTAAGTCGTGCCA+AGG 0.653849 7_4:-15695264 None:intergenic
TGAGAAGAGAAAAGGAACAG+AGG 0.654298 7_4:+15693642 Msa1141950:five_prime_UTR
GCATGAGCTGAGCGATCGTG+TGG 0.658125 7_4:+15695297 Msa1141950:CDS
ACAATTCTTGTGAACTACAA+GGG 0.663306 7_4:+15695737 Msa1141950:CDS
GTGGGAACCCAAAAGCCTTG+AGG 0.668086 7_4:+15696200 Msa1141950:CDS
CTTCAGAGAAGAGAAGAGCA+TGG 0.668770 7_4:+15693981 Msa1141950:five_prime_UTR
GGAACCCAAAAGCCTTGAGG+AGG 0.669169 7_4:+15696203 Msa1141950:CDS
AAGAGCCACTAAAGATGCAG+GGG 0.671111 7_4:+15695898 Msa1141950:CDS
AAAGCATTGCAAATCAACAA+GGG 0.678487 7_4:+15693900 Msa1141950:five_prime_UTR
CACACTAGAGAGGAGAGAAG+GGG 0.681269 7_4:+15693951 Msa1141950:five_prime_UTR
ACTTTGTGAGGAAGGCCTCG+AGG 0.709581 7_4:-15696840 None:intergenic
GGCAAAGGAGAGACAAAGGG+CGG 0.714758 7_4:+15693705 Msa1141950:five_prime_UTR
TTGCTGGTATCAATGTAATG+CGG 0.715918 7_4:+15695924 Msa1141950:CDS
GACACTTTCATACGACACTG+AGG 0.726186 7_4:+15696255 Msa1141950:CDS
ACAGATGCGAGAGATTGCAG+AGG 0.728184 7_4:+15695808 Msa1141950:CDS
TCTGCAGTGGAAGAAACCAG+TGG 0.733951 7_4:+15696900 Msa1141950:CDS
GCTGGAGACACTCACCTAGG+CGG 0.737514 7_4:+15696106 Msa1141950:CDS
GCTGATGCTAAGATGGACAG+GGG 0.747969 7_4:+15696403 Msa1141950:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATTAATAGTTTTAGTAAA+GGG + chr7_4:15694470-15694489 Msa1141950:intron 10.0%
!! TAAATTAATCTTAATTAGTT+TGG + chr7_4:15694678-15694697 Msa1141950:intron 10.0%
!! TATTCTTACAATTAAAAATA+AGG + chr7_4:15696063-15696082 Msa1141950:CDS 10.0%
!!! AAAAGTTGTTTTGTTTTATA+CGG + chr7_4:15693684-15693703 Msa1141950:five_prime_UTR 15.0%
!!! ACTAAAACTATTAATTTGTT+TGG - chr7_4:15694467-15694486 None:intergenic 15.0%
!!! CAAATTAATAGTTTTAGTAA+AGG + chr7_4:15694469-15694488 Msa1141950:intron 15.0%
!! AATAAAAAATTAAACAATGC+TGG - chr7_4:15695471-15695490 None:intergenic 15.0%
!! ATTACAATAACTAATGAAAA+TGG + chr7_4:15696936-15696955 Msa1141950:CDS 15.0%
!! AAAAAGAAGAAAGAAGAAAA+AGG + chr7_4:15693801-15693820 Msa1141950:five_prime_UTR 20.0%
!! AAAAGAAGAAAGAAGAAAAA+GGG + chr7_4:15693802-15693821 Msa1141950:five_prime_UTR 20.0%
!!! TTAAATTTGTCATCTTTTGT+TGG + chr7_4:15694019-15694038 Msa1141950:intron 20.0%
!!! ATTGATTTTATCACATGATT+AGG + chr7_4:15694212-15694231 Msa1141950:intron 20.0%
!!! ATCAAACAAAGTGTTAATTT+AGG + chr7_4:15694417-15694436 Msa1141950:intron 20.0%
!! TTAATTAGAACTAGACATAA+AGG + chr7_4:15694518-15694537 Msa1141950:intron 20.0%
!! TTAACAATTAACACTAACAT+CGG - chr7_4:15694908-15694927 None:intergenic 20.0%
!!! TTTGAATTGACTTCAAAATT+TGG - chr7_4:15695170-15695189 None:intergenic 20.0%
!! ATTCAAATCTTCTATACAAA+CGG + chr7_4:15695183-15695202 Msa1141950:five_prime_UTR 20.0%
!! ATATTATACATCACCATATT+AGG + chr7_4:15695516-15695535 Msa1141950:intron 20.0%
!! TATTATACATCACCATATTA+GGG + chr7_4:15695517-15695536 Msa1141950:intron 20.0%
!! GTTGATATGAAAATGAAAAT+AGG + chr7_4:15695585-15695604 Msa1141950:intron 20.0%
!!! AATATTTTTATCTTTGACCT+TGG + chr7_4:15696019-15696038 Msa1141950:CDS 20.0%
!!! ATCAACTCTAAAATGATAAA+GGG + chr7_4:15697140-15697159 Msa1141950:CDS 20.0%
!! TTTTTCTCTTCTTCTTTATG+CGG - chr7_4:15693668-15693687 None:intergenic 25.0%
!!! ACCTTTTTGAAACAAACAAT+TGG + chr7_4:15693759-15693778 Msa1141950:five_prime_UTR 25.0%
!! AAGAAGAAAAAGGGTTTTTA+CGG + chr7_4:15693811-15693830 Msa1141950:five_prime_UTR 25.0%
!! TGAATAGCTAACTATTTTCT+AGG + chr7_4:15694100-15694119 Msa1141950:intron 25.0%
!! GAATAGCTAACTATTTTCTA+GGG + chr7_4:15694101-15694120 Msa1141950:intron 25.0%
! ATCAATAGAACAAACTTGAT+TGG - chr7_4:15694198-15694217 None:intergenic 25.0%
! AACTGATTATTAACATGCAA+AGG - chr7_4:15694248-15694267 None:intergenic 25.0%
! TGATTGGAAGACTAAAATTA+AGG - chr7_4:15694347-15694366 None:intergenic 25.0%
!! GAAAAGCATAAAGTGTAAAA+AGG - chr7_4:15694375-15694394 None:intergenic 25.0%
!!! AATAGTTTTAGTAAAGGGAT+TGG + chr7_4:15694475-15694494 Msa1141950:intron 25.0%
!! TACTTGTTAATTAATCCATG+TGG + chr7_4:15694701-15694720 Msa1141950:intron 25.0%
! AAAAGATTATTGTATCCACA+TGG - chr7_4:15694719-15694738 None:intergenic 25.0%
!!! AGTATTGTAGTATTTTGGAA+AGG - chr7_4:15695128-15695147 None:intergenic 25.0%
!!! TGTAGAGTATTGTAGTATTT+TGG - chr7_4:15695133-15695152 None:intergenic 25.0%
! GAAATTATTCACAATGATCA+AGG + chr7_4:15695319-15695338 Msa1141950:CDS 25.0%
! TTGCATCTGCAAAAAATTAA+GGG - chr7_4:15695622-15695641 None:intergenic 25.0%
! TTTGCATCTGCAAAAAATTA+AGG - chr7_4:15695623-15695642 None:intergenic 25.0%
! TGTGAATCATTAAAATAAGC+AGG - chr7_4:15695875-15695894 None:intergenic 25.0%
! TGTCTTGATTAAAGTTTATG+AGG + chr7_4:15696752-15696771 Msa1141950:CDS 25.0%
! GTCTTGATTAAAGTTTATGA+GGG + chr7_4:15696753-15696772 Msa1141950:CDS 25.0%
! ATTGTAAAACATACTTTGTG+AGG - chr7_4:15696855-15696874 None:intergenic 25.0%
!!! AATTTCATTTTTATTGCCAC+TGG - chr7_4:15696919-15696938 None:intergenic 25.0%
! ATGAAAATGGAAGATTATCA+AGG + chr7_4:15696949-15696968 Msa1141950:CDS 25.0%
! TGAAAATGGAAGATTATCAA+GGG + chr7_4:15696950-15696969 Msa1141950:CDS 25.0%
!!! GATCAACTCTAAAATGATAA+AGG + chr7_4:15697139-15697158 Msa1141950:CDS 25.0%
!!! TGTTTTGTTTTATACGGCAA+AGG + chr7_4:15693690-15693709 Msa1141950:five_prime_UTR 30.0%
GCCAATTGTTTGTTTCAAAA+AGG - chr7_4:15693763-15693782 None:intergenic 30.0%
! AGAAGAAAAAGGGTTTTTAC+GGG + chr7_4:15693812-15693831 Msa1141950:five_prime_UTR 30.0%
! ACCTTACTGTGAAGTAATTT+TGG + chr7_4:15693845-15693864 Msa1141950:five_prime_UTR 30.0%
! CCTTACTGTGAAGTAATTTT+GGG + chr7_4:15693846-15693865 Msa1141950:five_prime_UTR 30.0%
! CTTACTGTGAAGTAATTTTG+GGG + chr7_4:15693847-15693866 Msa1141950:five_prime_UTR 30.0%
!! TAAAGCATTGCAAATCAACA+AGG + chr7_4:15693899-15693918 Msa1141950:five_prime_UTR 30.0%
!! AAAGCATTGCAAATCAACAA+GGG + chr7_4:15693900-15693919 Msa1141950:five_prime_UTR 30.0%
!!! CTAACTATTTTCTAGGGTTT+AGG + chr7_4:15694107-15694126 Msa1141950:intron 30.0%
GTGTAAAAAGGTTGAATGAT+TGG - chr7_4:15694363-15694382 None:intergenic 30.0%
AACAAGTAGTAGAATAGCTT+CGG - chr7_4:15694647-15694666 None:intergenic 30.0%
!! GAGTACAAGAAGTATTTGTT+GGG - chr7_4:15694788-15694807 None:intergenic 30.0%
!! TGAGTACAAGAAGTATTTGT+TGG - chr7_4:15694789-15694808 None:intergenic 30.0%
AAATACTTCTTGTACTCACT+CGG + chr7_4:15694791-15694810 Msa1141950:intron 30.0%
AATACTAGTCACATTGAAGA+AGG + chr7_4:15694944-15694963 Msa1141950:intron 30.0%
ACGATGGCAAAAAAATATGA+GGG + chr7_4:15695223-15695242 Msa1141950:exon 30.0%
TAAATTACCAAAGACAGTGT+TGG - chr7_4:15695434-15695453 None:intergenic 30.0%
AAAGTAAACAAGCCCTAATA+TGG - chr7_4:15695532-15695551 None:intergenic 30.0%
!!! AATTTTTTGCAGATGCAAAG+AGG + chr7_4:15695624-15695643 Msa1141950:intron 30.0%
TTGTAGTTCACAAGAATTGT+CGG - chr7_4:15695737-15695756 None:intergenic 30.0%
ACAATTCTTGTGAACTACAA+GGG + chr7_4:15695737-15695756 Msa1141950:CDS 30.0%
! ATTTTTATCTTTGACCTTGG+CGG + chr7_4:15696022-15696041 Msa1141950:CDS 30.0%
AAATAAGGTCTTTGATGTCA+AGG + chr7_4:15696078-15696097 Msa1141950:CDS 30.0%
! TGAAGACTTTGATAACAGAA+TGG + chr7_4:15696129-15696148 Msa1141950:CDS 30.0%
AAGAACAAAGAGGATATTAG+TGG + chr7_4:15696181-15696200 Msa1141950:CDS 30.0%
AGAACAAAGAGGATATTAGT+GGG + chr7_4:15696182-15696201 Msa1141950:CDS 30.0%
TACCTTGATATATAGCATCT+AGG - chr7_4:15696291-15696310 None:intergenic 30.0%
! ACTTTTGTTCATCAATCACT+CGG + chr7_4:15696314-15696333 Msa1141950:CDS 30.0%
CATATTCAATTGCTCAAACT+TGG - chr7_4:15696341-15696360 None:intergenic 30.0%
CAAGTTTGAGCAATTGAATA+TGG + chr7_4:15696339-15696358 Msa1141950:CDS 30.0%
! TATCCATACACTTTTCAAAG+AGG - chr7_4:15696366-15696385 None:intergenic 30.0%
TAAAACATACTTTGTGAGGA+AGG - chr7_4:15696851-15696870 None:intergenic 30.0%
! TGTTTTACAATAGATGCTGA+TGG + chr7_4:15696864-15696883 Msa1141950:CDS 30.0%
AAGGGAAAAAGTTTGATTGA+TGG + chr7_4:15697158-15697177 Msa1141950:CDS 30.0%
TCTCATGTGATGTAGTTTAG+CGG - chr7_4:15693627-15693646 None:intergenic 35.0%
ACATCACATGAGAAGAGAAA+AGG + chr7_4:15693634-15693653 Msa1141950:five_prime_UTR 35.0%
! TGATAATCCTTGGTGACTAA+GGG + chr7_4:15693728-15693747 Msa1141950:five_prime_UTR 35.0%
CCCAAAATTACTTCACAGTA+AGG - chr7_4:15693849-15693868 None:intergenic 35.0%
TCGGTGAAATTGAATTCAAG+AGG + chr7_4:15693870-15693889 Msa1141950:five_prime_UTR 35.0%
!!! ACTATTTTCTAGGGTTTAGG+TGG + chr7_4:15694110-15694129 Msa1141950:intron 35.0%
TCTAGTCTCAATTGTCCTAT+AGG - chr7_4:15694307-15694326 None:intergenic 35.0%
GTAAATCAACAAAGAGAGAG+AGG - chr7_4:15694578-15694597 None:intergenic 35.0%
CACGATGGCAAAAAAATATG+AGG + chr7_4:15695222-15695241 Msa1141950:exon 35.0%
TTAGTGAAAGCAACACAAGA+AGG - chr7_4:15695358-15695377 None:intergenic 35.0%
TTGCTTTCACTAATTCCCAA+AGG + chr7_4:15695365-15695384 Msa1141950:CDS 35.0%
! GTTTGAAGAAGCCTGATTTT+TGG - chr7_4:15695407-15695426 None:intergenic 35.0%
ATGCAAAGAGGTTAATTGGA+AGG + chr7_4:15695636-15695655 Msa1141950:CDS 35.0%
CAGTAGATCATTCTGAACAA+TGG - chr7_4:15695675-15695694 None:intergenic 35.0%
TTCAGAATGATCTACTGTTG+TGG + chr7_4:15695678-15695697 Msa1141950:CDS 35.0%
GACAATTCTTGTGAACTACA+AGG + chr7_4:15695736-15695755 Msa1141950:CDS 35.0%
GTAATCACTGCATTCTTAAC+AGG - chr7_4:15695848-15695867 None:intergenic 35.0%
TTGCTGGTATCAATGTAATG+CGG + chr7_4:15695924-15695943 Msa1141950:CDS 35.0%
!! CACAATTAGCTCTCTTTTGA+AGG - chr7_4:15695988-15696007 None:intergenic 35.0%
AATGGTGAATCACTTTGTGA+AGG + chr7_4:15696147-15696166 Msa1141950:CDS 35.0%
TATAGCATCTAGGTCAATTG+TGG - chr7_4:15696281-15696300 None:intergenic 35.0%
GACCTAGATGCTATATATCA+AGG + chr7_4:15696286-15696305 Msa1141950:CDS 35.0%
! CTTTTGTTCATCAATCACTC+GGG + chr7_4:15696315-15696334 Msa1141950:CDS 35.0%
! CTATTACAGGAGTTTTTCCA+GGG + chr7_4:15696484-15696503 Msa1141950:CDS 35.0%
! TATTACAGGAGTTTTTCCAG+GGG + chr7_4:15696485-15696504 Msa1141950:CDS 35.0%
ATTTCGATACCAAGTGACAA+TGG - chr7_4:15696636-15696655 None:intergenic 35.0%
! TGTCACTTGGTATCGAAATT+AGG + chr7_4:15696637-15696656 Msa1141950:intron 35.0%
! GTCACTTGGTATCGAAATTA+GGG + chr7_4:15696638-15696657 Msa1141950:intron 35.0%
! TCACTTGGTATCGAAATTAG+GGG + chr7_4:15696639-15696658 Msa1141950:intron 35.0%
TAGGGGAGATATTATGAATG+TGG + chr7_4:15696656-15696675 Msa1141950:intron 35.0%
TTATGAATGTGGTGATTCCA+AGG + chr7_4:15696667-15696686 Msa1141950:intron 35.0%
TATTCTTTAGTGCTCTTGAC+AGG - chr7_4:15696705-15696724 None:intergenic 35.0%
CTTTAATCAAGACAGTGCTT+TGG - chr7_4:15696746-15696765 None:intergenic 35.0%
!!! ACAACCTGTTGGGTTTTTTT+AGG + chr7_4:15696799-15696818 Msa1141950:CDS 35.0%
AGATCCTAAAAAAACCCAAC+AGG - chr7_4:15696806-15696825 None:intergenic 35.0%
AGTTGAAGCAAGAAATGCTT+TGG + chr7_4:15697040-15697059 Msa1141950:CDS 35.0%
TGAGAAGAGAAAAGGAACAG+AGG + chr7_4:15693642-15693661 Msa1141950:five_prime_UTR 40.0%
! GTGATAATCCTTGGTGACTA+AGG + chr7_4:15693727-15693746 Msa1141950:five_prime_UTR 40.0%
!!! CTGTGAAGTAATTTTGGGGT+CGG + chr7_4:15693851-15693870 Msa1141950:five_prime_UTR 40.0%
AACAAAGAGAGAGAGGTCTA+GGG - chr7_4:15694571-15694590 None:intergenic 40.0%
GTAAAAAGTAACGACGAGGT+AGG - chr7_4:15694617-15694636 None:intergenic 40.0%
CCAAGTAAAAAGTAACGACG+AGG - chr7_4:15694621-15694640 None:intergenic 40.0%
!!! CCTCGTCGTTACTTTTTACT+TGG + chr7_4:15694618-15694637 Msa1141950:intron 40.0%
! CACTAACATCGGTTGTTTGA+AGG - chr7_4:15694897-15694916 None:intergenic 40.0%
CTTCAATGTGATAGCTAGTG+TGG - chr7_4:15694992-15695011 None:intergenic 40.0%
ACTAGCTATCACATTGAAGC+AGG + chr7_4:15694993-15695012 Msa1141950:intron 40.0%
CATTGAAGCAGGAAAAGTCA+TGG + chr7_4:15695004-15695023 Msa1141950:intron 40.0%
GAAAAGTCATGGCTTCTGAT+AGG + chr7_4:15695015-15695034 Msa1141950:intron 40.0%
TTCATTCACTTGAAGCACGA+TGG + chr7_4:15695207-15695226 Msa1141950:exon 40.0%
AAATATGAGGGAGTTGCGAT+CGG + chr7_4:15695235-15695254 Msa1141950:CDS 40.0%
ACTAATTCCCAAAGGCTGAT+TGG + chr7_4:15695373-15695392 Msa1141950:CDS 40.0%
!! TACCAAAGACAGTGTTGGTT+GGG - chr7_4:15695429-15695448 None:intergenic 40.0%
!! TTACCAAAGACAGTGTTGGT+TGG - chr7_4:15695430-15695449 None:intergenic 40.0%
GCAGATGCAAAGAGGTTAAT+TGG + chr7_4:15695632-15695651 Msa1141950:intron 40.0%
!!! AGAGATTGCAGAGGCATTTT+TGG + chr7_4:15695817-15695836 Msa1141950:CDS 40.0%
AATATCCCCTGCATCTTTAG+TGG - chr7_4:15695906-15695925 None:intergenic 40.0%
ACTTTGTGAAGGAGTTCAAG+AGG + chr7_4:15696158-15696177 Msa1141950:CDS 40.0%
GTTCAAGAGGAAGAACAAAG+AGG + chr7_4:15696171-15696190 Msa1141950:CDS 40.0%
! GGACCTCTTTGAAAAGTGTA+TGG + chr7_4:15696360-15696379 Msa1141950:CDS 40.0%
TTGTCTTGCTGATGCTAAGA+TGG + chr7_4:15696396-15696415 Msa1141950:CDS 40.0%
GTAGATGATGTTGTCCTTGT+TGG + chr7_4:15696430-15696449 Msa1141950:CDS 40.0%
CTAGGATTCCTAAAGTGAGA+CGG + chr7_4:15696461-15696480 Msa1141950:CDS 40.0%
TGTAATAGCCGTCTCACTTT+AGG - chr7_4:15696472-15696491 None:intergenic 40.0%
TAAAGTGAGACGGCTATTAC+AGG + chr7_4:15696471-15696490 Msa1141950:CDS 40.0%
! GCTATTACAGGAGTTTTTCC+AGG + chr7_4:15696483-15696502 Msa1141950:CDS 40.0%
! AGAGTGTTCCATTCCAATCT+TGG + chr7_4:15696593-15696612 Msa1141950:CDS 40.0%
TCGCAAAACCAAGATTGGAA+TGG - chr7_4:15696604-15696623 None:intergenic 40.0%
ACATCTCGCAAAACCAAGAT+TGG - chr7_4:15696609-15696628 None:intergenic 40.0%
GATGTTACACCATTGTCACT+TGG + chr7_4:15696624-15696643 Msa1141950:intron 40.0%
TTTAGTGCTCTTGACAGGAA+TGG - chr7_4:15696700-15696719 None:intergenic 40.0%
! AATTTTGAACGTCTCTGCAG+TGG + chr7_4:15696887-15696906 Msa1141950:CDS 40.0%
AGAGATTGAGAGAATGATCC+AGG + chr7_4:15696974-15696993 Msa1141950:CDS 40.0%
! TTTGATTGATGGCAACCAAC+AGG + chr7_4:15697169-15697188 Msa1141950:CDS 40.0%
ACACAAATGCATCTTCCTGT+TGG - chr7_4:15697187-15697206 None:intergenic 40.0%
GGAAGATGCATTTGTGTTTG+TGG + chr7_4:15697190-15697209 Msa1141950:CDS 40.0%
ATACGGCAAAGGAGAGACAA+AGG + chr7_4:15693701-15693720 Msa1141950:five_prime_UTR 45.0%
TACGGCAAAGGAGAGACAAA+GGG + chr7_4:15693702-15693721 Msa1141950:five_prime_UTR 45.0%
! ATCCTTGGTGACTAAGGGAA+TGG + chr7_4:15693733-15693752 Msa1141950:five_prime_UTR 45.0%
AACCATTCCCTTAGTCACCA+AGG - chr7_4:15693738-15693757 None:intergenic 45.0%
ACACACTACACACACTAGAG+AGG + chr7_4:15693941-15693960 Msa1141950:five_prime_UTR 45.0%
ACACACTAGAGAGGAGAGAA+GGG + chr7_4:15693950-15693969 Msa1141950:five_prime_UTR 45.0%
CTTCAGAGAAGAGAAGAGCA+TGG + chr7_4:15693981-15694000 Msa1141950:five_prime_UTR 45.0%
GAGCATGGTGTAGAGATTGA+AGG + chr7_4:15693996-15694015 Msa1141950:five_prime_UTR 45.0%
ATCGTACTGCAGTTAACTGG+TGG + chr7_4:15694280-15694299 Msa1141950:intron 45.0%
! CAGTTAACTGGTGGACCTAT+AGG + chr7_4:15694289-15694308 Msa1141950:intron 45.0%
CAACAAAGAGAGAGAGGTCT+AGG - chr7_4:15694572-15694591 None:intergenic 45.0%
CTTACTCGTGTGTTGGAGTA+TGG + chr7_4:15695275-15695294 Msa1141950:CDS 45.0%
AGCATCACCAATCAGCCTTT+GGG - chr7_4:15695383-15695402 None:intergenic 45.0%
! TGGTGATGCTGCCAAAAATC+AGG + chr7_4:15695393-15695412 Msa1141950:CDS 45.0%
AACCCAACCAACACTGTCTT+TGG + chr7_4:15695424-15695443 Msa1141950:CDS 45.0%
TCTACTGTTGTGGCCCTTTA+AGG + chr7_4:15695688-15695707 Msa1141950:CDS 45.0%
!! TGGCCCTTTAAGGTCATATC+CGG + chr7_4:15695698-15695717 Msa1141950:CDS 45.0%
GCACCGGATATGACCTTAAA+GGG - chr7_4:15695704-15695723 None:intergenic 45.0%
! AGCACCGGATATGACCTTAA+AGG - chr7_4:15695705-15695724 None:intergenic 45.0%
TCTTGTGAACTACAAGGGTG+AGG + chr7_4:15695742-15695761 Msa1141950:CDS 45.0%
GAAGAGCCACTAAAGATGCA+GGG + chr7_4:15695897-15695916 Msa1141950:CDS 45.0%
AAGAGCCACTAAAGATGCAG+GGG + chr7_4:15695898-15695917 Msa1141950:CDS 45.0%
AAAGATGCAGGGGATATTGC+TGG + chr7_4:15695908-15695927 Msa1141950:CDS 45.0%
AATCAATGAACCGACTGCAG+CGG + chr7_4:15695949-15695968 Msa1141950:CDS 45.0%
TTTATCTTTGACCTTGGCGG+TGG + chr7_4:15696025-15696044 Msa1141950:CDS 45.0%
TATCAAAGTCTTCACCGCCT+AGG - chr7_4:15696123-15696142 None:intergenic 45.0%
! TCAACCTCCTCAAGGCTTTT+GGG - chr7_4:15696210-15696229 None:intergenic 45.0%
GACACTTTCATACGACACTG+AGG + chr7_4:15696255-15696274 Msa1141950:CDS 45.0%
TTGCTGATGCTAAGATGGAC+AGG + chr7_4:15696401-15696420 Msa1141950:CDS 45.0%
TGCTGATGCTAAGATGGACA+GGG + chr7_4:15696402-15696421 Msa1141950:CDS 45.0%
GATGATGTTGTCCTTGTTGG+TGG + chr7_4:15696433-15696452 Msa1141950:CDS 45.0%
!! TCCTTGTTGGTGGTTCTTCT+AGG + chr7_4:15696443-15696462 Msa1141950:CDS 45.0%
TCCTAGAAGAACCACCAACA+AGG - chr7_4:15696447-15696466 None:intergenic 45.0%
TCTTGCATAATTCCTTCCCC+TGG - chr7_4:15696504-15696523 None:intergenic 45.0%
!! CAAGAGCATTAACCCTGATG+AGG + chr7_4:15696519-15696538 Msa1141950:CDS 45.0%
!! ACAGGAATGGAAGTGTTCCT+TGG - chr7_4:15696687-15696706 None:intergenic 45.0%
AGCTAGTGAGAACAACCTGT+TGG + chr7_4:15696788-15696807 Msa1141950:CDS 45.0%
GCTAGTGAGAACAACCTGTT+GGG + chr7_4:15696789-15696808 Msa1141950:CDS 45.0%
CCTTGAAATTGTCAGCTTCC+TGG - chr7_4:15696995-15697014 None:intergenic 45.0%
CCAGGAAGCTGACAATTTCA+AGG + chr7_4:15696992-15697011 Msa1141950:CDS 45.0%
CAAAGGGCGGTGATAATCCT+TGG + chr7_4:15693718-15693737 Msa1141950:five_prime_UTR 50.0%
CACACACTAGAGAGGAGAGA+AGG + chr7_4:15693949-15693968 Msa1141950:five_prime_UTR 50.0%
CACACTAGAGAGGAGAGAAG+GGG + chr7_4:15693951-15693970 Msa1141950:five_prime_UTR 50.0%
GCGATCGTACTGCAGTTAAC+TGG + chr7_4:15694277-15694296 Msa1141950:intron 50.0%
GTTGCGATCGGAATTGACCT+TGG + chr7_4:15695247-15695266 Msa1141950:CDS 50.0%
CAGCATCACCAATCAGCCTT+TGG - chr7_4:15695384-15695403 None:intergenic 50.0%
!! TGTCGGTTTGTCATCAGCAC+CGG - chr7_4:15695720-15695739 None:intergenic 50.0%
CAATCTCTCGCATCTGTGAG+AGG - chr7_4:15695805-15695824 None:intergenic 50.0%
ACAGATGCGAGAGATTGCAG+AGG + chr7_4:15695808-15695827 Msa1141950:CDS 50.0%
CGAAGAGCCACTAAAGATGC+AGG + chr7_4:15695896-15695915 Msa1141950:CDS 50.0%
CATCAAAAGTTCCACCGCCA+AGG - chr7_4:15696039-15696058 None:intergenic 50.0%
TTTGATGTCAAGGCCACTGC+TGG + chr7_4:15696088-15696107 Msa1141950:CDS 50.0%
! CTCAACCTCCTCAAGGCTTT+TGG - chr7_4:15696211-15696230 None:intergenic 50.0%
GTTGAGAACTGCCTGTGAGA+GGG + chr7_4:15696225-15696244 Msa1141950:CDS 50.0%
! TGTCCTTTTTGCCCTCTCAC+AGG - chr7_4:15696239-15696258 None:intergenic 50.0%
GCTGATGCTAAGATGGACAG+GGG + chr7_4:15696403-15696422 Msa1141950:CDS 50.0%
! ACAGGAGTTTTTCCAGGGGA+AGG + chr7_4:15696489-15696508 Msa1141950:CDS 50.0%
CGTAAGCAACAGCCTCATCA+GGG - chr7_4:15696534-15696553 None:intergenic 50.0%
! CCTGATGAGGCTGTTGCTTA+CGG + chr7_4:15696532-15696551 Msa1141950:CDS 50.0%
TGCTTACGGTGCAGCTATTC+AGG + chr7_4:15696546-15696565 Msa1141950:CDS 50.0%
TCTGCAGTGGAAGAAACCAG+TGG + chr7_4:15696900-15696919 Msa1141950:CDS 50.0%
GGCAAAGGAGAGACAAAGGG+CGG + chr7_4:15693705-15693724 Msa1141950:five_prime_UTR 55.0%
CACACGAGTAAGTCGTGCCA+AGG - chr7_4:15695267-15695286 None:intergenic 55.0%
GGCACGACTTACTCGTGTGT+TGG + chr7_4:15695268-15695287 Msa1141950:CDS 55.0%
TATGCAAGAGCCGCTGCAGT+CGG - chr7_4:15695962-15695981 None:intergenic 55.0%
ACTGCAGCGGCTCTTGCATA+CGG + chr7_4:15695962-15695981 Msa1141950:CDS 55.0%
TAGGTGAGTGTCTCCAGCAG+TGG - chr7_4:15696104-15696123 None:intergenic 55.0%
ACTGCTGGAGACACTCACCT+AGG + chr7_4:15696103-15696122 Msa1141950:CDS 55.0%
GTGGGAACCCAAAAGCCTTG+AGG + chr7_4:15696200-15696219 Msa1141950:CDS 55.0%
GGAACCCAAAAGCCTTGAGG+AGG + chr7_4:15696203-15696222 Msa1141950:CDS 55.0%
GGCAGTTCTCAACCTCCTCA+AGG - chr7_4:15696218-15696237 None:intergenic 55.0%
GGTTGAGAACTGCCTGTGAG+AGG + chr7_4:15696224-15696243 Msa1141950:CDS 55.0%
CTGCCTGTGAGAGGGCAAAA+AGG + chr7_4:15696233-15696252 Msa1141950:CDS 55.0%
CCGTAAGCAACAGCCTCATC+AGG - chr7_4:15696535-15696554 None:intergenic 55.0%
ACTTTGTGAGGAAGGCCTCG+AGG - chr7_4:15696843-15696862 None:intergenic 55.0%
! GAGAGAGAGGTCTAGGGTCG+CGG - chr7_4:15694565-15694584 None:intergenic 60.0%
GCATGAGCTGAGCGATCGTG+TGG + chr7_4:15695297-15695316 Msa1141950:CDS 60.0%
GCTGGAGACACTCACCTAGG+CGG + chr7_4:15696106-15696125 Msa1141950:CDS 60.0%
TCAGTTCCCCGTGCACCTCG+AGG + chr7_4:15696825-15696844 Msa1141950:CDS 65.0%
AGGAAGGCCTCGAGGTGCAC+GGG - chr7_4:15696835-15696854 None:intergenic 65.0%
GAGGAAGGCCTCGAGGTGCA+CGG - chr7_4:15696836-15696855 None:intergenic 65.0%
GGAAGGCCTCGAGGTGCACG+GGG - chr7_4:15696834-15696853 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr7_4 gene 15693620 15697229 15693620 ID=Msa1141950;Name=Msa1141950
chr7_4 mRNA 15693620 15697229 15693620 ID=Msa1141950-mRNA-1;Parent=Msa1141950;Name=Msa1141950-mRNA-1;_AED=0.20;_eAED=0.20;_QI=507|0.66|0.5|1|0.66|0.5|4|0|579
chr7_4 exon 15693620 15694017 15693620 ID=Msa1141950-mRNA-1:exon:3587;Parent=Msa1141950-mRNA-1
chr7_4 exon 15695117 15695445 15695117 ID=Msa1141950-mRNA-1:exon:3588;Parent=Msa1141950-mRNA-1
chr7_4 exon 15695636 15696614 15695636 ID=Msa1141950-mRNA-1:exon:3589;Parent=Msa1141950-mRNA-1
chr7_4 exon 15696689 15697229 15696689 ID=Msa1141950-mRNA-1:exon:3590;Parent=Msa1141950-mRNA-1
chr7_4 five_prime_UTR 15693620 15694017 15693620 ID=Msa1141950-mRNA-1:five_prime_utr;Parent=Msa1141950-mRNA-1
chr7_4 five_prime_UTR 15695117 15695225 15695117 ID=Msa1141950-mRNA-1:five_prime_utr;Parent=Msa1141950-mRNA-1
chr7_4 CDS 15695226 15695445 15695226 ID=Msa1141950-mRNA-1:cds;Parent=Msa1141950-mRNA-1
chr7_4 CDS 15695636 15696614 15695636 ID=Msa1141950-mRNA-1:cds;Parent=Msa1141950-mRNA-1
chr7_4 CDS 15696689 15697229 15696689 ID=Msa1141950-mRNA-1:cds;Parent=Msa1141950-mRNA-1
Gene Sequence

>Msa1141950

ATGGCAAAAAAATATGAGGGAGTTGCGATCGGAATTGACCTTGGCACGACTTACTCGTGTGTTGGAGTATGGCATGAGCTGAGCGATCGTGTGGAAATTATTCACAATGATCAAGGAAACAAAACAACACCTTCTTGTGTTGCTTTCACTAATTCCCAAAGGCTGATTGGTGATGCTGCCAAAAATCAGGCTTCTTCAAACCCAACCAACACTGTCTTTGATGCAAAGAGGTTAATTGGAAGGAAATATAGTGATTCCATTGTTCAGAATGATCTACTGTTGTGGCCCTTTAAGGTCATATCCGGTGCTGATGACAAACCGACAATTCTTGTGAACTACAAGGGTGAGGAAAAGCACTTTGTTGCTGAAGAAATATCATCTGTGATCCTCTCACAGATGCGAGAGATTGCAGAGGCATTTTTGGAGTCACCTGTTAAGAATGCAGTGATTACTGTACCTGCTTATTTTAATGATTCACAACGAAGAGCCACTAAAGATGCAGGGGATATTGCTGGTATCAATGTAATGCGGATAATCAATGAACCGACTGCAGCGGCTCTTGCATACGGCCTTCAAAAGAGAGCTAATTGTGTTGATAAGAGAAATATTTTTATCTTTGACCTTGGCGGTGGAACTTTTGATGTGTCTATTCTTACAATTAAAAATAAGGTCTTTGATGTCAAGGCCACTGCTGGAGACACTCACCTAGGCGGTGAAGACTTTGATAACAGAATGGTGAATCACTTTGTGAAGGAGTTCAAGAGGAAGAACAAAGAGGATATTAGTGGGAACCCAAAAGCCTTGAGGAGGTTGAGAACTGCCTGTGAGAGGGCAAAAAGGACACTTTCATACGACACTGAGGCCACAATTGACCTAGATGCTATATATCAAGGTATTGACTTTTGTTCATCAATCACTCGGGCCAAGTTTGAGCAATTGAATATGGACCTCTTTGAAAAGTGTATGGATACTGTTAATAGTTGTCTTGCTGATGCTAAGATGGACAGGGGTAGTGTAGATGATGTTGTCCTTGTTGGTGGTTCTTCTAGGATTCCTAAAGTGAGACGGCTATTACAGGAGTTTTTCCAGGGGAAGGAATTATGCAAGAGCATTAACCCTGATGAGGCTGTTGCTTACGGTGCAGCTATTCAGGCTGCTTTGCATAGTGAAAGCGTTAAGAGTGTTCCATTCCAATCTTGGAACACTTCCATTCCTGTCAAGAGCACTAAAGAATATGTTACTTGTGAAGATGACCAAAGCACTGTCTTGATTAAAGTTTATGAGGGTGAGAGATTAAAAGCTAGTGAGAACAACCTGTTGGGTTTTTTTAGGATCTCAGTTCCCCGTGCACCTCGAGGCCTTCCTCACAAAGTATGTTTTACAATAGATGCTGATGGAATTTTGAACGTCTCTGCAGTGGAAGAAACCAGTGGCAATAAAAATGAAATTACAATAACTAATGAAAATGGAAGATTATCAAGGGAAGAGATTGAGAGAATGATCCAGGAAGCTGACAATTTCAAGGCTCAAGACATGAAGTTCAAAAAGAAAGTTGAAGCAAGAAATGCTTTGGATGATTATCTTTACAAAGTAAGAACAGTAATGAACGATAGTGTTAGCTCAAAGCTCACACTTGTAGAAAAAGTGAAGATCAACTCTAAAATGATAAAGGGAAAAAGTTTGATTGATGGCAACCAACAGGAAGATGCATTTGTGTTTGTGGACTTTTTGAAAGCTTGA
Protein sequence

>Msa1141950

MAKKYEGVAIGIDLGTTYSCVGVWHELSDRVEIIHNDQGNKTTPSCVAFTNSQRLIGDAAKNQASSNPTNTVFDAKRLIGRKYSDSIVQNDLLLWPFKVISGADDKPTILVNYKGEEKHFVAEEISSVILSQMREIAEAFLESPVKNAVITVPAYFNDSQRRATKDAGDIAGINVMRIINEPTAAALAYGLQKRANCVDKRNIFIFDLGGGTFDVSILTIKNKVFDVKATAGDTHLGGEDFDNRMVNHFVKEFKRKNKEDISGNPKALRRLRTACERAKRTLSYDTEATIDLDAIYQGIDFCSSITRAKFEQLNMDLFEKCMDTVNSCLADAKMDRGSVDDVVLVGGSSRIPKVRRLLQEFFQGKELCKSINPDEAVAYGAAIQAALHSESVKSVPFQSWNTSIPVKSTKEYVTCEDDQSTVLIKVYEGERLKASENNLLGFFRISVPRAPRGLPHKVCFTIDADGILNVSAVEETSGNKNEITITNENGRLSREEIERMIQEADNFKAQDMKFKKKVEARNALDDYLYKVRTVMNDSVSSKLTLVEKVKINSKMIKGKSLIDGNQQEDAFVFVDFLKA*