Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1151520 | MCI27438.1 | 86.408 | 103 | 13 | 1 | 1 | 102 | 1 | 103 | 6.45e-55 | 176 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1151520 | sp|Q8RXN6|PRP31_ARATH | 64.706 | 102 | 34 | 2 | 2 | 103 | 291 | 390 | 2.48e-27 | 107 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1151520 | A0A392QT26 | 86.408 | 103 | 13 | 1 | 1 | 102 | 1 | 103 | 3.08e-55 | 176 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0766630 | Msa1151520 | 0.800566 | 1.357090e-48 | -8.615850e-47 |
| Msa0142220 | Msa1151520 | 0.811150 | 8.084283e-51 | -8.615850e-47 |
| Msa1121490 | Msa1151520 | 0.802954 | 4.390819e-49 | -8.615850e-47 |
| Msa1135220 | Msa1151520 | 0.811562 | 6.580019e-51 | -8.615850e-47 |
| Msa1151520 | Msa1284680 | 0.803664 | 3.129491e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1151520 | MtrunA17_Chr7g0228641 | 76.699 | 103 | 22 | 2 | 1 | 103 | 290 | 390 | 1.83e-45 | 152 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1151520 | AT1G60170.1 | 64.706 | 102 | 34 | 2 | 2 | 103 | 291 | 390 | 2.52e-28 | 107 |
Find 26 sgRNAs with CRISPR-Local
Find 32 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGGGACCCATCGGGGAAAAC+TGG | 0.305205 | 7_4:-36126448 | Msa1151520:CDS |
| GTTGCAGAAGAGAGCTGTTT+AGG | 0.313833 | 7_4:-36126226 | Msa1151520:CDS |
| ATCAGGCACTGGAAGTGGTT+TGG | 0.364236 | 7_4:+36126357 | None:intergenic |
| TCAGGCACTGGAAGTGGTTT+GGG | 0.368103 | 7_4:+36126358 | None:intergenic |
| AGAGAGGTGGTCGTAGGCTT+AGG | 0.405617 | 7_4:-36126315 | Msa1151520:CDS |
| ATTTCAGGACATGTCAAATC+TGG | 0.415688 | 7_4:+36126189 | None:intergenic |
| GAATTGCATCCGATTAGCTT+TGG | 0.420471 | 7_4:+36126252 | None:intergenic |
| AGTGGTTTGGGTCGCTTAGC+AGG | 0.424208 | 7_4:+36126370 | None:intergenic |
| TTAATACATGGGGACCCATC+GGG | 0.463460 | 7_4:-36126457 | Msa1151520:CDS |
| GGAAGTCAGCCAAAGCTAAT+CGG | 0.467914 | 7_4:-36126261 | Msa1151520:CDS |
| AAAGCTAATCGGATGCAATT+CGG | 0.491444 | 7_4:-36126250 | Msa1151520:CDS |
| AGAAGAATGCAATGGCAGAT+AGG | 0.496400 | 7_4:-36126282 | Msa1151520:CDS |
| TCAGCATCAGGCACTGGAAG+TGG | 0.519633 | 7_4:+36126352 | None:intergenic |
| TTAGGCTCAGCATCAGGCAC+TGG | 0.527148 | 7_4:+36126346 | None:intergenic |
| GCACGAGTAGACTTAATACA+TGG | 0.545454 | 7_4:-36126469 | Msa1151520:CDS |
| TTGGGTCGCTTAGCAGGAGA+AGG | 0.546598 | 7_4:+36126376 | None:intergenic |
| TAAGTCTACTCGTGCAGCAA+GGG | 0.572657 | 7_4:+36126477 | None:intergenic |
| TCCGCACTAAAATTGAAAAG+TGG | 0.575441 | 7_4:-36126405 | Msa1151520:CDS |
| CTTAATACATGGGGACCCAT+CGG | 0.592411 | 7_4:-36126458 | Msa1151520:CDS |
| AGAAGAAGAGAGGTGGTCGT+AGG | 0.602525 | 7_4:-36126321 | Msa1151520:CDS |
| TCGTAGGCTTAGGAAAATGA+AGG | 0.605286 | 7_4:-36126305 | Msa1151520:CDS |
| TTAAGTCTACTCGTGCAGCA+AGG | 0.636964 | 7_4:+36126476 | None:intergenic |
| CACGAGTAGACTTAATACAT+GGG | 0.649909 | 7_4:-36126468 | Msa1151520:CDS |
| TAATACATGGGGACCCATCG+GGG | 0.662126 | 7_4:-36126456 | Msa1151520:CDS |
| AATGAAGGAGAAGAATGCAA+TGG | 0.688085 | 7_4:-36126290 | Msa1151520:CDS |
| ACGAGTAGACTTAATACATG+GGG | 0.718746 | 7_4:-36126467 | Msa1151520:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| CACGAGTAGACTTAATACAT+GGG | - | chr7_4:36126235-36126254 | Msa1151520:CDS | 35.0% | |
| ACGAGTAGACTTAATACATG+GGG | - | chr7_4:36126236-36126255 | Msa1151520:CDS | 35.0% | |
| TCCGCACTAAAATTGAAAAG+TGG | - | chr7_4:36126298-36126317 | Msa1151520:CDS | 35.0% | |
| !!! | GCCACTTTTCAATTTTAGTG+CGG | + | chr7_4:36126302-36126321 | None:intergenic | 35.0% |
| AATGAAGGAGAAGAATGCAA+TGG | - | chr7_4:36126413-36126432 | Msa1151520:CDS | 35.0% | |
| AAAGCTAATCGGATGCAATT+CGG | - | chr7_4:36126453-36126472 | Msa1151520:CDS | 35.0% | |
| GCACGAGTAGACTTAATACA+TGG | - | chr7_4:36126234-36126253 | Msa1151520:CDS | 40.0% | |
| !! | TTCTTTTTAGGCTCAGCATC+AGG | + | chr7_4:36126366-36126385 | None:intergenic | 40.0% |
| GAGCCTAAAAAGAAGAAGAG+AGG | - | chr7_4:36126372-36126391 | Msa1151520:CDS | 40.0% | |
| ! | CCACCTCTCTTCTTCTTTTT+AGG | + | chr7_4:36126378-36126397 | None:intergenic | 40.0% |
| CCTAAAAAGAAGAAGAGAGG+TGG | - | chr7_4:36126375-36126394 | Msa1151520:CDS | 40.0% | |
| TCGTAGGCTTAGGAAAATGA+AGG | - | chr7_4:36126398-36126417 | Msa1151520:CDS | 40.0% | |
| AGAAGAATGCAATGGCAGAT+AGG | - | chr7_4:36126421-36126440 | Msa1151520:CDS | 40.0% | |
| !! | GAATTGCATCCGATTAGCTT+TGG | + | chr7_4:36126454-36126473 | None:intergenic | 40.0% |
| TAAGTCTACTCGTGCAGCAA+GGG | + | chr7_4:36126229-36126248 | None:intergenic | 45.0% | |
| TTAAGTCTACTCGTGCAGCA+AGG | + | chr7_4:36126230-36126249 | None:intergenic | 45.0% | |
| CTTAATACATGGGGACCCAT+CGG | - | chr7_4:36126245-36126264 | Msa1151520:CDS | 45.0% | |
| TTAATACATGGGGACCCATC+GGG | - | chr7_4:36126246-36126265 | Msa1151520:CDS | 45.0% | |
| ! | TTGTTCCAGTTTTCCCCGAT+GGG | + | chr7_4:36126263-36126282 | None:intergenic | 45.0% |
| GGAAGTCAGCCAAAGCTAAT+CGG | - | chr7_4:36126442-36126461 | Msa1151520:CDS | 45.0% | |
| GTTGCAGAAGAGAGCTGTTT+AGG | - | chr7_4:36126477-36126496 | Msa1151520:CDS | 45.0% | |
| TAATACATGGGGACCCATCG+GGG | - | chr7_4:36126247-36126266 | Msa1151520:CDS | 50.0% | |
| ! | GTTGTTCCAGTTTTCCCCGA+TGG | + | chr7_4:36126264-36126283 | None:intergenic | 50.0% |
| !! | TCAGGCACTGGAAGTGGTTT+GGG | + | chr7_4:36126348-36126367 | None:intergenic | 50.0% |
| !! | ATCAGGCACTGGAAGTGGTT+TGG | + | chr7_4:36126349-36126368 | None:intergenic | 50.0% |
| AGAAGAAGAGAGGTGGTCGT+AGG | - | chr7_4:36126382-36126401 | Msa1151520:CDS | 50.0% | |
| TTGGGTCGCTTAGCAGGAGA+AGG | + | chr7_4:36126330-36126349 | None:intergenic | 55.0% | |
| ! | AGTGGTTTGGGTCGCTTAGC+AGG | + | chr7_4:36126336-36126355 | None:intergenic | 55.0% |
| !! | TCAGCATCAGGCACTGGAAG+TGG | + | chr7_4:36126354-36126373 | None:intergenic | 55.0% |
| TTAGGCTCAGCATCAGGCAC+TGG | + | chr7_4:36126360-36126379 | None:intergenic | 55.0% | |
| AGAGAGGTGGTCGTAGGCTT+AGG | - | chr7_4:36126388-36126407 | Msa1151520:CDS | 55.0% | |
| GGGGACCCATCGGGGAAAAC+TGG | - | chr7_4:36126255-36126274 | Msa1151520:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_4 | gene | 36126192 | 36126533 | 36126192 | ID=Msa1151520;Name=Msa1151520 |
| chr7_4 | mRNA | 36126192 | 36126533 | 36126192 | ID=Msa1151520-mRNA-1;Parent=Msa1151520;Name=Msa1151520-mRNA-1;_AED=0.38;_eAED=0.38;_QI=0|-1|0|1|-1|1|1|0|113 |
| chr7_4 | exon | 36126192 | 36126533 | 36126192 | ID=Msa1151520-mRNA-1:exon:8488;Parent=Msa1151520-mRNA-1 |
| chr7_4 | CDS | 36126192 | 36126533 | 36126192 | ID=Msa1151520-mRNA-1:cds;Parent=Msa1151520-mRNA-1 |
| Gene Sequence |
| Protein sequence |
