AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1156190

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1156190 MtrunA17_Chr7g0249561 99.231 130 1 0 1 130 1 130 3.46e-93 265
Msa1156190 MtrunA17_Chr7g0249801 52.066 121 48 3 10 129 7 118 1.25e-35 119
Msa1156190 MtrunA17_Chr4g0076301 51.948 77 37 0 54 130 47 123 2.65e-21 82.8
Msa1156190 MtrunA17_Chr8g0385881 41.772 79 44 2 53 130 61 138 3.45e-11 57.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1156190 AT1G65290.1 72.180 133 27 3 1 130 1 126 6.69e-60 181
Msa1156190 AT2G44620.1 53.488 129 52 4 1 129 1 121 6.31e-36 120
Msa1156190 AT5G47630.1 37.097 124 71 3 10 130 8 127 1.40e-19 79.0
Msa1156190 AT5G47630.2 37.097 124 71 3 10 130 8 127 1.40e-19 79.0
Msa1156190 AT5G47630.3 37.097 124 71 3 10 130 8 127 1.40e-19 79.0

Find 54 sgRNAs with CRISPR-Local

Find 151 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTTAGAAGGATCAACTTTC+TGG 0.153197 7_4:+44434722 None:intergenic
ATCTTGTCTGCTTCATTATC+AGG 0.242778 7_4:+44432948 None:intergenic
GAAACGACGAAGGAAGAAAT+TGG 0.274656 7_4:+44434819 None:intergenic
TATCCAATCCAAGGTCATTC+TGG 0.280875 7_4:+44433027 None:intergenic
TCCCCAGCCAAACACACTTT+TGG 0.287162 7_4:+44432737 None:intergenic
CCAGAAAGTTGATCCTTCTA+AGG 0.310928 7_4:-44434722 Msa1156190:intron
GTCGTTTCAGCGAAGAGGTT+AGG 0.325470 7_4:-44434804 Msa1156190:CDS
TGACCTTGGATTGGATAGTT+TGG 0.330609 7_4:-44433021 Msa1156190:CDS
ATGGCTCTTGAGGAAGAGTT+TGG 0.348440 7_4:-44432981 Msa1156190:CDS
TCGTTTCAGCGAAGAGGTTA+GGG 0.350830 7_4:-44434803 Msa1156190:CDS
CTAAATCCCAAAAGTGTGTT+TGG 0.352275 7_4:-44432744 Msa1156190:three_prime_UTR
AGGAATGAATATACCTTAGA+AGG 0.366704 7_4:+44434709 None:intergenic
TTTACGATTACAGAATCTTA+AGG 0.391212 7_4:-44432615 Msa1156190:three_prime_UTR
ATGAATTCCACTACTTTGCC+TGG 0.402676 7_4:+44432883 None:intergenic
ATCCCAAAAGTGTGTTTGGC+TGG 0.403903 7_4:-44432740 Msa1156190:three_prime_UTR
TGGCTCTTGAGGAAGAGTTT+GGG 0.415460 7_4:-44432980 Msa1156190:CDS
AGTATTGGCATTTCATAACT+TGG 0.421265 7_4:-44432682 Msa1156190:three_prime_UTR
ACAATGGCGGCGAGAAGAAC+CGG 0.449215 7_4:-44434919 Msa1156190:CDS
TTTCCAGAATGACCTTGGAT+TGG 0.459097 7_4:-44433030 Msa1156190:CDS
AAAGGCGATTGGTGGTTCAA+TGG 0.465586 7_4:+44434853 None:intergenic
GTAATTGATCAGAGGTAAAC+CGG 0.480151 7_4:+44434900 None:intergenic
TCCCAAAAGTGTGTTTGGCT+GGG 0.488684 7_4:-44432739 Msa1156190:three_prime_UTR
AAACGACGAAGGAAGAAATT+GGG 0.492698 7_4:+44434820 None:intergenic
GCTCATTTCCAGAATGACCT+TGG 0.503501 7_4:-44433035 Msa1156190:CDS
TGAATTCCACTACTTTGCCT+GGG 0.511311 7_4:+44432884 None:intergenic
GTTCAATTTAAGCAAACAAG+TGG 0.519121 7_4:+44432784 None:intergenic
TCATTCTGGAAATGAGCACT+TGG 0.522843 7_4:+44433041 None:intergenic
CTTCATTGCATCTCATCCCC+AGG 0.529562 7_4:-44432901 Msa1156190:CDS
GTCTTGTTTCATGTTATGTG+AGG 0.530605 7_4:-44432536 Msa1156190:three_prime_UTR
CCTTCGTCGTTTCAGCGAAG+AGG 0.534191 7_4:-44434809 Msa1156190:CDS
AAATTGGGAAGAAGACGAAA+AGG 0.543498 7_4:+44434835 None:intergenic
GAGAATCAACTTGCACTCTC+AGG 0.548584 7_4:+44434878 None:intergenic
AAGTAAAATACAACTCGAGC+TGG 0.554852 7_4:+44432710 None:intergenic
CATTCTGGAAATGAGCACTT+GGG 0.569313 7_4:+44433042 None:intergenic
CTCATCCCCAGGCAAAGTAG+TGG 0.584414 7_4:-44432890 Msa1156190:exon
CCCAAAAGTGTGTTTGGCTG+GGG 0.584451 7_4:-44432738 Msa1156190:three_prime_UTR
TCTTGTTTCATGTTATGTGA+GGG 0.585940 7_4:-44432535 Msa1156190:three_prime_UTR
AGCAGAGAGAGAGAAGAGTG+AGG 0.586439 7_4:-44434957 Msa1156190:five_prime_UTR
GAAGAAGACGAAAAGGCGAT+TGG 0.587345 7_4:+44434842 None:intergenic
GAAGACGAAAAGGCGATTGG+TGG 0.593083 7_4:+44434845 None:intergenic
TATTCATTTCATTCATCGCG+TGG 0.597536 7_4:-44434987 Msa1156190:five_prime_UTR
AGAGATTGTGATGGCTCTTG+AGG 0.598690 7_4:-44432991 Msa1156190:CDS
CGTTTCAGCGAAGAGGTTAG+GGG 0.604330 7_4:-44434802 Msa1156190:CDS
GCGATGAATGAAATGAATAG+TGG 0.615247 7_4:+44434991 None:intergenic
GGATGCTGTAGAGATTGTGA+TGG 0.619499 7_4:-44433000 Msa1156190:CDS
GAATTCCACTACTTTGCCTG+GGG 0.620707 7_4:+44432885 None:intergenic
TTTGATATCTACAACAATGG+CGG 0.632027 7_4:-44434932 Msa1156190:exon
CATCCAAACTATCCAATCCA+AGG 0.636485 7_4:+44433018 None:intergenic
TACGTTTCTCGACAAATCTG+AGG 0.640218 7_4:-44434779 Msa1156190:CDS
ATTCTGGAAATGAGCACTTG+GGG 0.645151 7_4:+44433043 None:intergenic
GCAAAGTAGTGGAATTCATG+AGG 0.659430 7_4:-44432879 Msa1156190:exon
CCTCTTCGCTGAAACGACGA+AGG 0.663136 7_4:+44434809 None:intergenic
ACTCTCAGGTAATTGATCAG+AGG 0.675792 7_4:+44434892 None:intergenic
TGAGCATACACTAAGAAGTG+AGG 0.685947 7_4:+44432580 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAATTATTTCCGATAATT+AGG - chr7_4:44432907-44432926 Msa1156190:CDS 15.0%
!! TTAACTTTATCTTTAGTTAT+AGG + chr7_4:44432984-44433003 None:intergenic 15.0%
!!! ATTGTTTTCATATAAGTTAT+AGG - chr7_4:44433008-44433027 Msa1156190:CDS 15.0%
!!! TTGTTTTCATATAAGTTATA+GGG - chr7_4:44433009-44433028 Msa1156190:CDS 15.0%
!!! TTTAACTTTATTTTACCTTT+TGG + chr7_4:44433119-44433138 None:intergenic 15.0%
!! TACAAAAATATATAAATCCT+TGG - chr7_4:44433515-44433534 Msa1156190:intron 15.0%
!! AACTAAAACAATGTTATTAT+CGG + chr7_4:44433672-44433691 None:intergenic 15.0%
!!! TATTTTACTTTTGTTAGTAT+TGG - chr7_4:44434783-44434802 Msa1156190:CDS 15.0%
!!! ATTTTTATTGACATGAAAAA+TGG + chr7_4:44434836-44434855 None:intergenic 15.0%
!! TGATGAATGAAACAATAATT+AGG + chr7_4:44432827-44432846 None:intergenic 20.0%
!!! GCTATTTTTATAAGCTATAT+TGG - chr7_4:44433160-44433179 Msa1156190:intron 20.0%
!!! TAAGCTAGATTATTTTCTTA+AGG + chr7_4:44433317-44433336 None:intergenic 20.0%
!!! ATTTTTGTATTGCATTTTAG+TGG + chr7_4:44433504-44433523 None:intergenic 20.0%
!!! AACATTGTTTTAGTTATATG+AGG - chr7_4:44433677-44433696 Msa1156190:intron 20.0%
!! AATCTTTCATCAAATTAAGT+AGG - chr7_4:44433715-44433734 Msa1156190:intron 20.0%
!! ATCTTTCATCAAATTAAGTA+GGG - chr7_4:44433716-44433735 Msa1156190:intron 20.0%
!! TCGCAAACAATAATATATTT+GGG + chr7_4:44433923-44433942 None:intergenic 20.0%
!! ATCGCAAACAATAATATATT+TGG + chr7_4:44433924-44433943 None:intergenic 20.0%
!! GTTTTTGATATCTACAACAA+TGG - chr7_4:44432545-44432564 Msa1156190:three_prime_UTR 25.0%
! GAAACGAATGATAATAATAG+AGG + chr7_4:44432794-44432813 None:intergenic 25.0%
! ATTAACGTGATGTTATTGTT+AGG - chr7_4:44432846-44432865 Msa1156190:three_prime_UTR 25.0%
! TCCAAACATTACCTAATTAT+CGG + chr7_4:44432921-44432940 None:intergenic 25.0%
! TAAGCTACTTATATACCAAA+AGG - chr7_4:44433101-44433120 Msa1156190:intron 25.0%
! AAAGTAGCTTATGAAATGTT+TGG + chr7_4:44433289-44433308 None:intergenic 25.0%
! AAACATTTCATAAGCTACTT+TGG - chr7_4:44433288-44433307 Msa1156190:intron 25.0%
! ATATAAGAATTGCTCATTGA+TGG + chr7_4:44433468-44433487 None:intergenic 25.0%
! CAATTCTTATATGAAGCTAA+AGG - chr7_4:44433476-44433495 Msa1156190:intron 25.0%
! TATAAATCCTTGGATTTGAT+TGG - chr7_4:44433525-44433544 Msa1156190:intron 25.0%
! AATTGAGTGTAATTCTTACA+TGG + chr7_4:44433593-44433612 None:intergenic 25.0%
!! ATGGCAATAATTCTCTTTTA+TGG + chr7_4:44433781-44433800 None:intergenic 25.0%
! AAGAGAATTATTGCCATAAA+AGG - chr7_4:44433784-44433803 Msa1156190:intron 25.0%
!! TCAAAAACAGTTTCCTTTTA+TGG + chr7_4:44433800-44433819 None:intergenic 25.0%
! AAGTGATTACTTCATTTAAG+AGG - chr7_4:44433977-44433996 Msa1156190:intron 25.0%
! TACTTCATTTAAGAGGATTA+AGG - chr7_4:44433984-44434003 Msa1156190:intron 25.0%
!!! TAACTTGTTTGAAAACAGTT+TGG + chr7_4:44434100-44434119 None:intergenic 25.0%
! ATAAACTTGTGAGACTAATT+GGG + chr7_4:44434212-44434231 None:intergenic 25.0%
! TATAAACTTGTGAGACTAAT+TGG + chr7_4:44434213-44434232 None:intergenic 25.0%
! TTTACGATTACAGAATCTTA+AGG - chr7_4:44434865-44434884 Msa1156190:CDS 25.0%
TTTGATATCTACAACAATGG+CGG - chr7_4:44432548-44432567 Msa1156190:three_prime_UTR 30.0%
AGGAATGAATATACCTTAGA+AGG + chr7_4:44432774-44432793 None:intergenic 30.0%
TCCGATAATTAGGTAATGTT+TGG - chr7_4:44432917-44432936 Msa1156190:CDS 30.0%
TTAGGTAATGTTTGGATCAA+CGG - chr7_4:44432925-44432944 Msa1156190:CDS 30.0%
AAAGCTGGTAGCAATATTAA+AGG + chr7_4:44433439-44433458 None:intergenic 30.0%
AACATAACCAATCAAATCCA+AGG + chr7_4:44433535-44433554 None:intergenic 30.0%
! TTGGATTTGATTGGTTATGT+TGG - chr7_4:44433534-44433553 Msa1156190:intron 30.0%
ACTGGTTAAGGATATGAAAA+AGG + chr7_4:44433875-44433894 None:intergenic 30.0%
AAGAAGTTAGAAACAGCTTA+TGG - chr7_4:44434157-44434176 Msa1156190:intron 30.0%
ACAAATAAACCAATCCAAAC+AGG - chr7_4:44434242-44434261 Msa1156190:intron 30.0%
CAAATAAACCAATCCAAACA+GGG - chr7_4:44434243-44434262 Msa1156190:intron 30.0%
ACTTCAAACAGTAAAGTGTA+AGG + chr7_4:44434301-44434320 None:intergenic 30.0%
!! TTGAAGTGTGTTTGTCATTT+TGG - chr7_4:44434314-44434333 Msa1156190:intron 30.0%
CTTATACTCTCTTGTTAACA+TGG - chr7_4:44434384-44434403 Msa1156190:intron 30.0%
GTTCAATTTAAGCAAACAAG+TGG + chr7_4:44434699-44434718 None:intergenic 30.0%
!! AGTATTGGCATTTCATAACT+TGG - chr7_4:44434798-44434817 Msa1156190:CDS 30.0%
! TCTTGTTTCATGTTATGTGA+GGG - chr7_4:44434945-44434964 Msa1156190:five_prime_UTR 30.0%
GCGATGAATGAAATGAATAG+TGG + chr7_4:44432492-44432511 None:intergenic 35.0%
TATTCATTTCATTCATCGCG+TGG - chr7_4:44432493-44432512 Msa1156190:three_prime_UTR 35.0%
GTAATTGATCAGAGGTAAAC+CGG + chr7_4:44432583-44432602 None:intergenic 35.0%
AAATTGGGAAGAAGACGAAA+AGG + chr7_4:44432648-44432667 None:intergenic 35.0%
AAACGACGAAGGAAGAAATT+GGG + chr7_4:44432663-44432682 None:intergenic 35.0%
TATAAGTTATAGGGCGTGTT+TGG - chr7_4:44433018-44433037 Msa1156190:CDS 35.0%
AGCTTATGAAATGTTTGGCT+TGG + chr7_4:44433284-44433303 None:intergenic 35.0%
CATCTACTCATGTAAGCATT+CGG + chr7_4:44433384-44433403 None:intergenic 35.0%
CTGGTAGCAATATTAAAGGA+AGG + chr7_4:44433435-44433454 None:intergenic 35.0%
GATGAAATTATCACTGCCAA+GGG - chr7_4:44433941-44433960 Msa1156190:intron 35.0%
! TGATAAATGGAAAGAAGCAC+AGG + chr7_4:44434412-44434431 None:intergenic 35.0%
TGTGCTTCTTTCCATTTATC+AGG - chr7_4:44434411-44434430 Msa1156190:intron 35.0%
ATCTTGTCTGCTTCATTATC+AGG + chr7_4:44434535-44434554 None:intergenic 35.0%
AGCAAAAAATGTCTTAGCGT+TGG + chr7_4:44434676-44434695 None:intergenic 35.0%
!!! CACACTTTTGGGATTTAGAA+GGG + chr7_4:44434734-44434753 None:intergenic 35.0%
!!! ACACACTTTTGGGATTTAGA+AGG + chr7_4:44434735-44434754 None:intergenic 35.0%
! CTAAATCCCAAAAGTGTGTT+TGG - chr7_4:44434736-44434755 Msa1156190:CDS 35.0%
AAGTAAAATACAACTCGAGC+TGG + chr7_4:44434773-44434792 None:intergenic 35.0%
!! GTCTTGTTTCATGTTATGTG+AGG - chr7_4:44434944-44434963 Msa1156190:five_prime_UTR 35.0%
ACTCTCAGGTAATTGATCAG+AGG + chr7_4:44432591-44432610 None:intergenic 40.0%
GAAACGACGAAGGAAGAAAT+TGG + chr7_4:44432664-44432683 None:intergenic 40.0%
TACGTTTCTCGACAAATCTG+AGG - chr7_4:44432701-44432720 Msa1156190:three_prime_UTR 40.0%
CCTTAGAAGGATCAACTTTC+TGG + chr7_4:44432761-44432780 None:intergenic 40.0%
! CCAGAAAGTTGATCCTTCTA+AGG - chr7_4:44432758-44432777 Msa1156190:three_prime_UTR 40.0%
GCTTATCTACGAGACTCTTT+GGG + chr7_4:44433252-44433271 None:intergenic 40.0%
AGCTTATCTACGAGACTCTT+TGG + chr7_4:44433253-44433272 None:intergenic 40.0%
CTAGCTTATAGACGTGTCAT+TGG - chr7_4:44433329-44433348 Msa1156190:intron 40.0%
ACATTGTTCAGGAGAGCTTA+TGG + chr7_4:44433361-44433380 None:intergenic 40.0%
TAAGCATTCGGACATTGTTC+AGG + chr7_4:44433372-44433391 None:intergenic 40.0%
CGATGAAATTATCACTGCCA+AGG - chr7_4:44433940-44433959 Msa1156190:intron 40.0%
CTTAAGAGAGATCAAACCCT+TGG + chr7_4:44433960-44433979 None:intergenic 40.0%
! TAAGCTATCCTAGAGAGCAT+AGG - chr7_4:44434133-44434152 Msa1156190:intron 40.0%
ACTTCTTTCCTATGCTCTCT+AGG + chr7_4:44434144-44434163 None:intergenic 40.0%
GTTTACACTATAGCCCTGTT+TGG + chr7_4:44434259-44434278 None:intergenic 40.0%
AGTACACAATTGCTTGCAGA+GGG + chr7_4:44434351-44434370 None:intergenic 40.0%
TAGTACACAATTGCTTGCAG+AGG + chr7_4:44434352-44434371 None:intergenic 40.0%
ACTTGGGGTAACCTGATAAA+TGG + chr7_4:44434425-44434444 None:intergenic 40.0%
! ATTCTGGAAATGAGCACTTG+GGG + chr7_4:44434440-44434459 None:intergenic 40.0%
! CATTCTGGAAATGAGCACTT+GGG + chr7_4:44434441-44434460 None:intergenic 40.0%
! TCATTCTGGAAATGAGCACT+TGG + chr7_4:44434442-44434461 None:intergenic 40.0%
TTTCCAGAATGACCTTGGAT+TGG - chr7_4:44434450-44434469 Msa1156190:intron 40.0%
TATCCAATCCAAGGTCATTC+TGG + chr7_4:44434456-44434475 None:intergenic 40.0%
! TGACCTTGGATTGGATAGTT+TGG - chr7_4:44434459-44434478 Msa1156190:intron 40.0%
CATCCAAACTATCCAATCCA+AGG + chr7_4:44434465-44434484 None:intergenic 40.0%
TGAATTCCACTACTTTGCCT+GGG + chr7_4:44434599-44434618 None:intergenic 40.0%
ATGAATTCCACTACTTTGCC+TGG + chr7_4:44434600-44434619 None:intergenic 40.0%
! GCAAAGTAGTGGAATTCATG+AGG - chr7_4:44434601-44434620 Msa1156190:intron 40.0%
TGAGCATACACTAAGAAGTG+AGG + chr7_4:44434903-44434922 None:intergenic 40.0%
GAGAATCAACTTGCACTCTC+AGG + chr7_4:44432605-44432624 None:intergenic 45.0%
! AAAGGCGATTGGTGGTTCAA+TGG + chr7_4:44432630-44432649 None:intergenic 45.0%
GAAGAAGACGAAAAGGCGAT+TGG + chr7_4:44432641-44432660 None:intergenic 45.0%
TCGTTTCAGCGAAGAGGTTA+GGG - chr7_4:44432677-44432696 Msa1156190:three_prime_UTR 45.0%
TTAAGTAGGGTCCTGCTAAC+TGG - chr7_4:44433729-44433748 Msa1156190:intron 45.0%
CTTGTGAGACTAATTGGGAG+AGG + chr7_4:44434207-44434226 None:intergenic 45.0%
CACTATAGCCCTGTTTGGAT+TGG + chr7_4:44434254-44434273 None:intergenic 45.0%
GCTCATTTCCAGAATGACCT+TGG - chr7_4:44434445-44434464 Msa1156190:intron 45.0%
GGATGCTGTAGAGATTGTGA+TGG - chr7_4:44434480-44434499 Msa1156190:intron 45.0%
AGAGATTGTGATGGCTCTTG+AGG - chr7_4:44434489-44434508 Msa1156190:intron 45.0%
ATGGCTCTTGAGGAAGAGTT+TGG - chr7_4:44434499-44434518 Msa1156190:intron 45.0%
TGGCTCTTGAGGAAGAGTTT+GGG - chr7_4:44434500-44434519 Msa1156190:intron 45.0%
GAATTCCACTACTTTGCCTG+GGG + chr7_4:44434598-44434617 None:intergenic 45.0%
!!! TTTTGGGATTTAGAAGGGCG+AGG + chr7_4:44434729-44434748 None:intergenic 45.0%
! ATCCCAAAAGTGTGTTTGGC+TGG - chr7_4:44434740-44434759 Msa1156190:CDS 45.0%
! TCCCAAAAGTGTGTTTGGCT+GGG - chr7_4:44434741-44434760 Msa1156190:CDS 45.0%
AGCAGAGAGAGAGAAGAGTG+AGG - chr7_4:44432523-44432542 Msa1156190:three_prime_UTR 50.0%
GAAGACGAAAAGGCGATTGG+TGG + chr7_4:44432638-44432657 None:intergenic 50.0%
GTCGTTTCAGCGAAGAGGTT+AGG - chr7_4:44432676-44432695 Msa1156190:three_prime_UTR 50.0%
CGTTTCAGCGAAGAGGTTAG+GGG - chr7_4:44432678-44432697 Msa1156190:three_prime_UTR 50.0%
!! CATTGATGGCGTCGTAAAGC+TGG + chr7_4:44433454-44433473 None:intergenic 50.0%
CATATCCTTAACCAGTGCCC+CGG - chr7_4:44433879-44433898 Msa1156190:intron 50.0%
ATATCCTTAACCAGTGCCCC+GGG - chr7_4:44433880-44433899 Msa1156190:intron 50.0%
CTTCATTGCATCTCATCCCC+AGG - chr7_4:44434579-44434598 Msa1156190:intron 50.0%
! CCCCAGCCAAACACACTTTT+GGG + chr7_4:44434745-44434764 None:intergenic 50.0%
! CCCAAAAGTGTGTTTGGCTG+GGG - chr7_4:44434742-44434761 Msa1156190:CDS 50.0%
! TCCCCAGCCAAACACACTTT+TGG + chr7_4:44434746-44434765 None:intergenic 50.0%
ACAATGGCGGCGAGAAGAAC+CGG - chr7_4:44432561-44432580 Msa1156190:three_prime_UTR 55.0%
CCTCTTCGCTGAAACGACGA+AGG + chr7_4:44432674-44432693 None:intergenic 55.0%
CCTTCGTCGTTTCAGCGAAG+AGG - chr7_4:44432671-44432690 Msa1156190:three_prime_UTR 55.0%
TATGCTTAACCAGTGCCCCG+GGG + chr7_4:44433758-44433777 None:intergenic 55.0%
GTATGCTTAACCAGTGCCCC+GGG + chr7_4:44433759-44433778 None:intergenic 55.0%
GGTATGCTTAACCAGTGCCC+CGG + chr7_4:44433760-44433779 None:intergenic 55.0%
TATCCTTAACCAGTGCCCCG+GGG - chr7_4:44433881-44433900 Msa1156190:intron 55.0%
AAATGCTAACCGTTGCCCCC+GGG + chr7_4:44433900-44433919 None:intergenic 55.0%
GAAATGCTAACCGTTGCCCC+CGG + chr7_4:44433901-44433920 None:intergenic 55.0%
CTCATCCCCAGGCAAAGTAG+TGG - chr7_4:44434590-44434609 Msa1156190:intron 55.0%
ATGCTTAACCAGTGCCCCGG+GGG + chr7_4:44433757-44433776 None:intergenic 60.0%
ATCCTTAACCAGTGCCCCGG+GGG - chr7_4:44433882-44433901 Msa1156190:intron 60.0%
AATGCTAACCGTTGCCCCCG+GGG + chr7_4:44433899-44433918 None:intergenic 60.0%
GTCCTGCTAACTGGTGCCCC+CGG - chr7_4:44433738-44433757 Msa1156190:intron 65.0%
TCCTGCTAACTGGTGCCCCC+GGG - chr7_4:44433739-44433758 Msa1156190:intron 65.0%
CCTGCTAACTGGTGCCCCCG+GGG - chr7_4:44433740-44433759 Msa1156190:intron 70.0%
!! TGCCCCCGGGGCACTGGTTA+AGG + chr7_4:44433752-44433771 None:intergenic 70.0%
AACCAGTGCCCCGGGGGCAA+CGG - chr7_4:44433888-44433907 Msa1156190:intron 70.0%
!! CCCCGGGGGCACCAGTTAGC+AGG + chr7_4:44433743-44433762 None:intergenic 75.0%
AACTGGTGCCCCCGGGGCAC+TGG - chr7_4:44433746-44433765 Msa1156190:intron 75.0%
AACCGTTGCCCCCGGGGCAC+TGG + chr7_4:44433893-44433912 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
chr7_4 gene 44432480 44435022 44432480 ID=Msa1156190;Name=Msa1156190
chr7_4 mRNA 44432480 44435022 44432480 ID=Msa1156190-mRNA-1;Parent=Msa1156190;Name=Msa1156190-mRNA-1;_AED=0.06;_eAED=0.06;_QI=84|1|1|1|1|1|2|413|130
chr7_4 exon 44432480 44433069 44432480 ID=Msa1156190-mRNA-1:exon:11368;Parent=Msa1156190-mRNA-1
chr7_4 exon 44434723 44435022 44434723 ID=Msa1156190-mRNA-1:exon:11367;Parent=Msa1156190-mRNA-1
chr7_4 five_prime_UTR 44434939 44435022 44434939 ID=Msa1156190-mRNA-1:five_prime_utr;Parent=Msa1156190-mRNA-1
chr7_4 CDS 44434723 44434938 44434723 ID=Msa1156190-mRNA-1:cds;Parent=Msa1156190-mRNA-1
chr7_4 CDS 44432893 44433069 44432893 ID=Msa1156190-mRNA-1:cds;Parent=Msa1156190-mRNA-1
chr7_4 three_prime_UTR 44432480 44432892 44432480 ID=Msa1156190-mRNA-1:three_prime_utr;Parent=Msa1156190-mRNA-1
Gene Sequence

>Msa1156190

ATGGCGGCGAGAAGAACCGGTTTACCTCTGATCAATTACCTGAGAGTGCAAGTTGATTCTCGTCCATTGAACCACCAATCGCCTTTTCGTCTTCTTCCCAATTTCTTCCTTCGTCGTTTCAGCGAAGAGGTTAGGGGTACGTTTCTCGACAAATCTGAGGTCACAGATCGCGTCGTTTCTTGCGTCAAAAACTTCCAGAAAGTTGATCCTTCTAAGGTTACCCCAAGTGCTCATTTCCAGAATGACCTTGGATTGGATAGTTTGGATGCTGTAGAGATTGTGATGGCTCTTGAGGAAGAGTTTGGGTTTGAGATTCCTGATAATGAAGCAGACAAGATCAACTCTGTTAATCTTGCAATTGACTTCATTGCATCTCATCCCCAGGCAAAGTAG
Protein sequence

>Msa1156190

MAARRTGLPLINYLRVQVDSRPLNHQSPFRLLPNFFLRRFSEEVRGTFLDKSEVTDRVVSCVKNFQKVDPSKVTPSAHFQNDLGLDSLDAVEIVMALEEEFGFEIPDNEADKINSVNLAIDFIASHPQAK*