AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0100730

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0100730 MtrunA17_Chr1g0148961 100.000 134 0 0 1 134 1 134 1.02e-93 266
Msa0100730 MtrunA17_Chr1g0148951 100.000 134 0 0 1 134 1 134 1.02e-93 266
Msa0100730 MtrunA17_Chr1g0184901 86.567 134 18 0 1 134 1 134 1.96e-73 215
Msa0100730 MtrunA17_Chr1g0211651 81.250 128 24 0 7 134 4 131 2.88e-71 209
Msa0100730 MtrunA17_Chr4g0024701 66.418 134 25 1 1 134 1 114 6.90e-47 147
Msa0100730 MtrunA17_Chr2g0319191 64.545 110 37 2 24 132 14 122 1.42e-40 132
Msa0100730 MtrunA17_Chr2g0319181 64.545 110 37 2 24 132 14 122 1.91e-40 131
Msa0100730 MtrunA17_Chr7g0268831 58.333 132 47 4 1 130 1 126 2.47e-40 131
Msa0100730 MtrunA17_Chr7g0273351 58.197 122 49 2 10 130 3 123 8.09e-40 130
Msa0100730 MtrunA17_Chr7g0273331 58.197 122 49 2 10 130 59 179 9.14e-39 129
Msa0100730 MtrunA17_Chr7g0259151 61.682 107 39 2 25 130 21 126 2.97e-38 126
Msa0100730 MtrunA17_Chr7g0272611 55.652 115 49 2 19 132 19 132 7.29e-37 123
Msa0100730 MtrunA17_Chr4g0031041 59.292 113 44 2 23 134 15 126 4.29e-36 121
Msa0100730 MtrunA17_Chr2g0327951 59.292 113 44 2 23 134 56 167 8.62e-36 121
Msa0100730 MtrunA17_Chr4g0035621 61.111 108 40 2 27 133 25 131 4.35e-35 118
Msa0100730 MtrunA17_Chr8g0379311 60.550 109 41 2 27 134 25 132 4.84e-35 118
Msa0100730 MtrunA17_Chr4g0032301 59.633 109 42 2 27 134 25 132 5.44e-35 118
Msa0100730 MtrunA17_Chr4g0031001 59.633 109 42 2 27 134 25 132 6.61e-35 118
Msa0100730 MtrunA17_Chr4g0031651 59.633 109 42 2 27 134 25 132 6.76e-35 118
Msa0100730 MtrunA17_Chr2g0327931 60.185 108 41 2 27 133 24 130 8.79e-35 118
Msa0100730 MtrunA17_Chr7g0273341 53.448 116 40 5 19 132 50 153 3.35e-28 101
Msa0100730 MtrunA17_Chr4g0031031 62.687 67 24 1 55 120 17 83 3.79e-20 79.3
Msa0100730 MtrunA17_Chr6g0449661 45.283 106 47 4 27 130 86 182 6.63e-19 78.6
Msa0100730 MtrunA17_Chr3g0119971 44.340 106 48 4 27 130 81 177 1.70e-18 77.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0100730 AT1G52740.1 88.060 134 16 0 1 134 1 134 5.27e-76 222
Msa0100730 AT3G54560.1 84.559 136 19 1 1 134 1 136 7.75e-72 211
Msa0100730 AT3G54560.2 84.559 136 19 1 1 134 1 136 7.75e-72 211
Msa0100730 AT2G38810.1 81.618 136 23 1 1 134 1 136 1.01e-71 211
Msa0100730 AT2G38810.4 81.618 136 23 1 1 134 1 136 1.01e-71 211
Msa0100730 AT2G38810.3 81.618 136 23 1 1 134 1 136 1.01e-71 211
Msa0100730 AT2G38810.2 81.618 136 23 1 1 134 1 136 1.01e-71 211
Msa0100730 AT4G13570.1 66.667 111 33 1 18 124 8 118 3.28e-45 143
Msa0100730 AT4G13570.2 75.556 90 22 0 35 124 35 124 3.59e-44 141
Msa0100730 AT4G27230.2 64.545 110 37 2 24 132 14 122 2.42e-40 131
Msa0100730 AT4G27230.1 64.545 110 37 2 24 132 14 122 2.42e-40 131
Msa0100730 AT5G54640.1 64.545 110 37 2 24 132 14 122 2.45e-40 131
Msa0100730 AT3G20670.1 65.741 108 35 2 24 130 14 120 6.89e-40 130
Msa0100730 AT1G08880.1 64.762 105 35 2 25 128 21 124 1.01e-39 130
Msa0100730 AT1G51060.1 63.636 110 38 2 24 132 14 122 1.11e-39 130
Msa0100730 AT1G54690.1 63.810 105 36 2 25 128 21 124 2.89e-39 129
Msa0100730 AT5G02560.1 61.947 113 41 2 23 134 21 132 1.19e-38 128
Msa0100730 AT5G27670.1 60.870 115 43 2 19 132 18 131 4.54e-37 124
Msa0100730 AT5G59870.1 61.261 111 41 2 23 132 21 130 3.64e-36 121
Msa0100730 AT5G02560.2 51.095 137 41 3 23 134 21 156 3.79e-33 115

Find 57 sgRNAs with CRISPR-Local

Find 244 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCTTGTCTTTGAGGTTTGC+TGG 0.259581 1_3:+7166064 None:intergenic
TCTTCTTGTCCTTGTCTTTG+AGG 0.316058 1_3:+7166056 None:intergenic
TTCCTCAAGAGCTGGTCTTC+AGG 0.343744 1_3:-7166020 Msa0100730:intron
TATGAACATTCATTCCTTTG+AGG 0.376752 1_3:+7163020 None:intergenic
GGTGATAACAACTTTATTTG+TGG 0.407928 1_3:-7162948 Msa0100730:three_prime_UTR
CAATGGCACATGGAAGAGTT+GGG 0.442531 1_3:-7163263 Msa0100730:CDS
ACATGGAAGAGTTGGGGCTA+CGG 0.468116 1_3:-7163256 Msa0100730:CDS
ACAATGGCACATGGAAGAGT+TGG 0.476378 1_3:-7163264 Msa0100730:CDS
AATATCACACAGTAAGAAAC+CGG 0.476767 1_3:+7162712 None:intergenic
GGAGCGAAGGGTTTGACAAC+CGG 0.477894 1_3:-7166094 Msa0100730:CDS
GTATTTAACTGCAGAGGTGT+TGG 0.485770 1_3:-7163205 Msa0100730:CDS
CTCATCAAAGGAACAATAGC+TGG 0.487626 1_3:-7163087 Msa0100730:CDS
TTGAAGTGCGAAAACATGTC+GGG 0.497395 1_3:-7166121 Msa0100730:exon
TGCATGTCTAGGATTTATGT+TGG 0.504995 1_3:-7162804 Msa0100730:three_prime_UTR
GGATAATTCGCTCCTTGCGT+TGG 0.505084 1_3:-7162927 Msa0100730:three_prime_UTR
ATAAGAGACTTGTGGATATG+AGG 0.507095 1_3:+7163051 None:intergenic
AACATAAATCCTAGACATGC+AGG 0.533027 1_3:+7162806 None:intergenic
AGACGATGGATACGTCCGAC+AGG 0.539450 1_3:+7163300 None:intergenic
AACTGCAGAGGTGTTGGAGT+TGG 0.540859 1_3:-7163199 Msa0100730:CDS
AGCTCTTGAGGAACGAGATG+TGG 0.542232 1_3:+7166030 None:intergenic
AACCTGAAGACCAGCTCTTG+AGG 0.543571 1_3:+7166018 None:intergenic
ACATCTCGTTCCTCAAGAGC+TGG 0.546311 1_3:-7166028 Msa0100730:CDS
TAATTCTCTCTAATTAACGC+AGG 0.546344 1_3:-7162999 Msa0100730:three_prime_UTR
GCAGAGGTGTTGGAGTTGGC+TGG 0.558822 1_3:-7163195 Msa0100730:CDS
CAATGCAAGCAAGGATCTGA+AGG 0.565081 1_3:-7163172 Msa0100730:CDS
TGCGAAAACATGTCGGGGAA+AGG 0.567757 1_3:-7166115 Msa0100730:exon
GAACTTGACACTCTCATCAA+AGG 0.571379 1_3:-7163099 Msa0100730:CDS
GTTGGCTGGCAATGCAAGCA+AGG 0.571379 1_3:-7163181 Msa0100730:CDS
AAATTCAGATAATCTAAAGA+AGG 0.572497 1_3:+7162886 None:intergenic
ACTTGTTGATAAGAGACTTG+TGG 0.572936 1_3:+7163043 None:intergenic
TCAGATAATCTAAAGAAGGA+TGG 0.582849 1_3:+7162890 None:intergenic
TGTCAAGTTCTTCGTCTCCA+CGG 0.583078 1_3:+7163112 None:intergenic
ATAGCAAGCTGTAGATGTCT+CGG 0.584334 1_3:+7163135 None:intergenic
TCTTATCAACAAGTCCTCAA+AGG 0.584732 1_3:-7163034 Msa0100730:CDS
CATCTACAGCTTGCTATCCG+TGG 0.586408 1_3:-7163129 Msa0100730:CDS
ACTGCTTGGCAATGGAGTAA+TGG 0.589145 1_3:-7162749 Msa0100730:three_prime_UTR
TCCATCGTCTTCTGAAGTCA+AGG 0.589423 1_3:-7163287 Msa0100730:CDS
GGTATGGTACCTGCATGTCT+AGG 0.591611 1_3:-7162815 Msa0100730:three_prime_UTR
TTTGAAGTGCGAAAACATGT+CGG 0.591882 1_3:-7166122 Msa0100730:exon
CCTCTGCAGTTAAATACTCT+AGG 0.598269 1_3:+7163211 None:intergenic
CATGTCGGGGAAAGGAGCGA+AGG 0.599837 1_3:-7166107 Msa0100730:CDS
AATTCTCTCTAATTAACGCA+GGG 0.600377 1_3:-7162998 Msa0100730:three_prime_UTR
GCTCTTGAGGAACGAGATGT+GGG 0.607406 1_3:+7166031 None:intergenic
AAAGGAACAATAGCTGGTGG+TGG 0.608922 1_3:-7163081 Msa0100730:CDS
CCTAGAGTATTTAACTGCAG+AGG 0.617705 1_3:-7163211 Msa0100730:CDS
AAGTCAAGGACAATGGCACA+TGG 0.622887 1_3:-7163273 Msa0100730:CDS
ATGTCGGGGAAAGGAGCGAA+GGG 0.624226 1_3:-7166106 Msa0100730:CDS
CAATCAATCAAACCAACGCA+AGG 0.626065 1_3:+7162915 None:intergenic
GACGATGGATACGTCCGACA+GGG 0.626692 1_3:+7163301 None:intergenic
TTCTACATACAGTTCCCTGT+CGG 0.628096 1_3:-7163315 Msa0100730:intron
ACCAGCAAACCTCAAAGACA+AGG 0.637885 1_3:-7166065 Msa0100730:CDS
AATGGCACATGGAAGAGTTG+GGG 0.645846 1_3:-7163262 Msa0100730:CDS
TGAAGTGCGAAAACATGTCG+GGG 0.649052 1_3:-7166120 Msa0100730:exon
TCTTCTGAAGTCAAGGACAA+TGG 0.655563 1_3:-7163280 Msa0100730:CDS
ATCAAAGGAACAATAGCTGG+TGG 0.661257 1_3:-7163084 Msa0100730:CDS
TCCTTGACTTCAGAAGACGA+TGG 0.668973 1_3:+7163286 None:intergenic
AGTTAAATACTCTAGGATAG+CGG 0.703360 1_3:+7163218 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATGTATTTTTTTGTTAAA+AGG - chr1_3:7163077-7163096 Msa0100730:CDS 10.0%
!! TTTATGATTTATAATATCAA+TGG - chr1_3:7163697-7163716 Msa0100730:intron 10.0%
!!! TTTTTTTGTTAAAAGGATAA+TGG - chr1_3:7163084-7163103 Msa0100730:CDS 15.0%
!!! TTTTTTGTTAAAAGGATAAT+GGG - chr1_3:7163085-7163104 Msa0100730:CDS 15.0%
!! ACAAAAAGAATAGTAAAAAA+CGG + chr1_3:7163364-7163383 None:intergenic 15.0%
!!! TTTTTTTGTTTGTTTTAAGT+AGG - chr1_3:7164425-7164444 Msa0100730:intron 15.0%
!! TATATTCATGCTAATATATA+TGG + chr1_3:7164784-7164803 None:intergenic 15.0%
!!! AGTATTATTTTATAACTGTT+TGG - chr1_3:7165035-7165054 Msa0100730:intron 15.0%
!!! AAAGGATTTTAATGTAATTT+CGG - chr1_3:7165934-7165953 Msa0100730:intron 15.0%
!!! AAAATTGTAGTTTGAGATTT+GGG - chr1_3:7163126-7163145 Msa0100730:CDS 20.0%
!!! TTTTTATCTAAGATGAAGTA+TGG - chr1_3:7163149-7163168 Msa0100730:CDS 20.0%
!!! TTTTATCTAAGATGAAGTAT+GGG - chr1_3:7163150-7163169 Msa0100730:CDS 20.0%
!!! TTTTTACCAAGATTGAAATT+TGG - chr1_3:7163190-7163209 Msa0100730:CDS 20.0%
!!! TTTTACCAAGATTGAAATTT+GGG - chr1_3:7163191-7163210 Msa0100730:CDS 20.0%
!! ATATATACACCTATTAAGAA+GGG + chr1_3:7163982-7164001 None:intergenic 20.0%
!! AATATATACACCTATTAAGA+AGG + chr1_3:7163983-7164002 None:intergenic 20.0%
!! ATATATTCAGTAATGCTAAA+TGG - chr1_3:7163996-7164015 Msa0100730:intron 20.0%
!! ATTATAAAAGATAGTCCATT+CGG + chr1_3:7164053-7164072 None:intergenic 20.0%
!! TGGAAAATGAAAAGATAATT+TGG + chr1_3:7164133-7164152 None:intergenic 20.0%
!!! CTTCGATATTGATTAATTAA+TGG + chr1_3:7164313-7164332 None:intergenic 20.0%
!! TTAATTAATCAATATCGAAG+TGG - chr1_3:7164313-7164332 Msa0100730:intron 20.0%
!! TTATAGAATCATATGTGAAT+GGG - chr1_3:7164450-7164469 Msa0100730:intron 20.0%
!!! GTTTTAAAAATTTGTCATGA+AGG + chr1_3:7164609-7164628 None:intergenic 20.0%
!!! TCATGACAAATTTTTAAAAC+AGG - chr1_3:7164609-7164628 Msa0100730:intron 20.0%
!!! CAAATTTTTAAAACAGGTTA+CGG - chr1_3:7164615-7164634 Msa0100730:intron 20.0%
!!! AAATTTTTAAAACAGGTTAC+GGG - chr1_3:7164616-7164635 Msa0100730:intron 20.0%
!!! ACAGATTTTCAAATATACAT+AGG + chr1_3:7165116-7165135 None:intergenic 20.0%
!! TATTGTATTTCTTTGATTCT+TGG - chr1_3:7165446-7165465 Msa0100730:intron 20.0%
!! AAATTCAGATAATCTAAAGA+AGG + chr1_3:7166020-7166039 None:intergenic 20.0%
!!! ATTTTTCTTCTCTTTTGATA+TGG - chr1_3:7166036-7166055 Msa0100730:CDS 20.0%
! AAAAAAACGATGAAGAGAAA+GGG + chr1_3:7162930-7162949 None:intergenic 25.0%
! AGTGAAAAAGGTTAAATTTG+AGG + chr1_3:7162973-7162992 None:intergenic 25.0%
!! GAAAATTGTAGTTTGAGATT+TGG - chr1_3:7163125-7163144 Msa0100730:CDS 25.0%
!! TTTATCTAAGATGAAGTATG+GGG - chr1_3:7163151-7163170 Msa0100730:CDS 25.0%
! CAGTGAAAAACTAATAACAT+GGG + chr1_3:7163271-7163290 None:intergenic 25.0%
! TCAGTGAAAAACTAATAACA+TGG + chr1_3:7163272-7163291 None:intergenic 25.0%
! CAGTTAAGCAAATTGTAAAT+GGG + chr1_3:7163295-7163314 None:intergenic 25.0%
! TCAGTTAAGCAAATTGTAAA+TGG + chr1_3:7163296-7163315 None:intergenic 25.0%
! AAAAGAATAGTAAAAAACGG+TGG + chr1_3:7163361-7163380 None:intergenic 25.0%
!!! AATTACCTTGTTTTGTTTCA+TGG - chr1_3:7163393-7163412 Msa0100730:intron 25.0%
! GAGAATTGAATAGTTGAATA+TGG - chr1_3:7163466-7163485 Msa0100730:intron 25.0%
!!! GGATGTTCATTTTATTTCTT+TGG - chr1_3:7163599-7163618 Msa0100730:intron 25.0%
! CAAAAGAAGAAATCAAATCA+TGG + chr1_3:7163644-7163663 None:intergenic 25.0%
!! GCAATTCATTTTACAACTTA+AGG + chr1_3:7163736-7163755 None:intergenic 25.0%
! AGAGAACATATGCAAAATAA+GGG + chr1_3:7163779-7163798 None:intergenic 25.0%
!!! TTTTCTTTTTTCGGGAAAAA+AGG + chr1_3:7163890-7163909 None:intergenic 25.0%
! CTATTATTTCCCTTCTTAAT+AGG - chr1_3:7163970-7163989 Msa0100730:intron 25.0%
!!! AAAAGATAATTTGGTGTAAG+GGG + chr1_3:7164124-7164143 None:intergenic 25.0%
!!! GAAAAGATAATTTGGTGTAA+GGG + chr1_3:7164125-7164144 None:intergenic 25.0%
!!! TGAAAAGATAATTTGGTGTA+AGG + chr1_3:7164126-7164145 None:intergenic 25.0%
! CAAAAAAACAATCAATCCAT+TGG + chr1_3:7164413-7164432 None:intergenic 25.0%
! GTTATAGAATCATATGTGAA+TGG - chr1_3:7164449-7164468 Msa0100730:intron 25.0%
! TATAGAATCATATGTGAATG+GGG - chr1_3:7164451-7164470 Msa0100730:intron 25.0%
! TTCAATGTTATGTTAGTTGT+AGG - chr1_3:7164659-7164678 Msa0100730:intron 25.0%
! ATTCAATGTTGAACAAGTAT+TGG - chr1_3:7164696-7164715 Msa0100730:intron 25.0%
!! AGATATCACATAATCTTTTG+TGG - chr1_3:7164829-7164848 Msa0100730:intron 25.0%
! AAAAAGACAAAATCATCACA+CGG + chr1_3:7165397-7165416 None:intergenic 25.0%
CAAAAAAACGATGAAGAGAA+AGG + chr1_3:7162931-7162950 None:intergenic 30.0%
CCAAAACAAAAGAGTGAAAA+AGG + chr1_3:7162985-7163004 None:intergenic 30.0%
!! CCTTTTTCACTCTTTTGTTT+TGG - chr1_3:7162982-7163001 Msa0100730:three_prime_UTR 30.0%
!!! ATGGGTTTTACTTTTTGTAG+AGG - chr1_3:7163103-7163122 Msa0100730:CDS 30.0%
CTTTAGATTAAAGACGTCTT+CGG + chr1_3:7163558-7163577 None:intergenic 30.0%
! ACGTCTTTAATCTAAAGTGT+TGG - chr1_3:7163561-7163580 Msa0100730:intron 30.0%
!! TGTTGGTGCTACATTATTTA+TGG - chr1_3:7163578-7163597 Msa0100730:intron 30.0%
!! AAATGAATTGCTCACTTTTG+AGG - chr1_3:7163745-7163764 Msa0100730:intron 30.0%
CAGAGAACATATGCAAAATA+AGG + chr1_3:7163780-7163799 None:intergenic 30.0%
! TATTTTGCATATGTTCTCTG+TGG - chr1_3:7163780-7163799 Msa0100730:intron 30.0%
TTGTTTGAAAAGCTTAGACA+AGG + chr1_3:7163813-7163832 None:intergenic 30.0%
TGGATATTAGAAGGGATATA+GGG - chr1_3:7163927-7163946 Msa0100730:intron 30.0%
AAGAAGGGAAATAATAGTTC+CGG + chr1_3:7163967-7163986 None:intergenic 30.0%
GATTTCAATCCAATGATCAT+TGG + chr1_3:7164283-7164302 None:intergenic 30.0%
TTAATCAATATCGAAGTGGT+TGG - chr1_3:7164317-7164336 Msa0100730:intron 30.0%
TAATCAATATCGAAGTGGTT+GGG - chr1_3:7164318-7164337 Msa0100730:intron 30.0%
TTGCAGTCAAGATTAGTAAT+TGG - chr1_3:7164371-7164390 Msa0100730:intron 30.0%
AATGTTATGTTAGTTGTAGG+AGG - chr1_3:7164662-7164681 Msa0100730:intron 30.0%
!! AATGTTGAACAAGTATTGGA+TGG - chr1_3:7164700-7164719 Msa0100730:intron 30.0%
! ATGGATTACATAAAACACAG+AGG + chr1_3:7165220-7165239 None:intergenic 30.0%
TTAGTAATCCAAAAACGAGA+AGG + chr1_3:7165246-7165265 None:intergenic 30.0%
!! TCGTTTTTGGATTACTAATG+TGG - chr1_3:7165248-7165267 Msa0100730:intron 30.0%
GTAAATCATGTTGCATTTGT+TGG - chr1_3:7165513-7165532 Msa0100730:intron 30.0%
ATGTAGAAAGATGTTCATGA+TGG + chr1_3:7165576-7165595 None:intergenic 30.0%
!! AGTTAAATACTCTAGGATAG+CGG + chr1_3:7165688-7165707 None:intergenic 30.0%
TATGAACATTCATTCCTTTG+AGG + chr1_3:7165886-7165905 None:intergenic 30.0%
TAATTCTCTCTAATTAACGC+AGG - chr1_3:7165904-7165923 Msa0100730:intron 30.0%
AATTCTCTCTAATTAACGCA+GGG - chr1_3:7165905-7165924 Msa0100730:intron 30.0%
ATTAACGCAGGGAAAAAAAA+AGG - chr1_3:7165916-7165935 Msa0100730:intron 30.0%
! GGTGATAACAACTTTATTTG+TGG - chr1_3:7165955-7165974 Msa0100730:intron 30.0%
TCAGATAATCTAAAGAAGGA+TGG + chr1_3:7166016-7166035 None:intergenic 30.0%
!! AACTAACATCTTCACTTTTG+TGG - chr1_3:7166067-7166086 Msa0100730:CDS 30.0%
! TTTGAAGTGCGAAAACATGT+CGG - chr1_3:7162781-7162800 Msa0100730:three_prime_UTR 35.0%
CACTTTAGCGATGTAACATA+AGG + chr1_3:7163031-7163050 None:intergenic 35.0%
GTACACCCAAATTTCAATCT+TGG + chr1_3:7163199-7163218 None:intergenic 35.0%
GATTTATTGTGCCACATTGA+TGG - chr1_3:7163314-7163333 Msa0100730:intron 35.0%
ATTCGCCATGAAACAAAACA+AGG + chr1_3:7163401-7163420 None:intergenic 35.0%
TTGAATATGGTCACATGTGT+CGG - chr1_3:7163479-7163498 Msa0100730:intron 35.0%
! ACTCGCGCATTTTCTTTTTT+CGG + chr1_3:7163899-7163918 None:intergenic 35.0%
GTGGATATTAGAAGGGATAT+AGG - chr1_3:7163926-7163945 Msa0100730:intron 35.0%
ATATTAGAAGGGATATAGGG+TGG - chr1_3:7163930-7163949 Msa0100730:intron 35.0%
AGAAGGGAAATAATAGTTCC+GGG + chr1_3:7163966-7163985 None:intergenic 35.0%
TAAAGCTTGTACAGTGTAAG+CGG + chr1_3:7164227-7164246 None:intergenic 35.0%
CTGTCATAACCAATGATCAT+TGG - chr1_3:7164271-7164290 Msa0100730:intron 35.0%
TGAAGGTTGACGACTTATAA+TGG + chr1_3:7164592-7164611 None:intergenic 35.0%
TTAGTTGTAGGAGGCTAAAT+TGG - chr1_3:7164671-7164690 Msa0100730:intron 35.0%
!!! TGTTTTAACTGTCAAGACCA+AGG + chr1_3:7164967-7164986 None:intergenic 35.0%
CAGAATTTCTTAGTGATCCA+CGG + chr1_3:7165001-7165020 None:intergenic 35.0%
ATGCTGTGTTCAATTAGACA+TGG + chr1_3:7165158-7165177 None:intergenic 35.0%
! TCCATATACCTTCTCGTTTT+TGG - chr1_3:7165235-7165254 Msa0100730:intron 35.0%
! TCCAAAAACGAGAAGGTATA+TGG + chr1_3:7165239-7165258 None:intergenic 35.0%
CCACTTGCAAAAAATTCTAG+TGG + chr1_3:7165421-7165440 None:intergenic 35.0%
!!! CCACTAGAATTTTTTGCAAG+TGG - chr1_3:7165418-7165437 Msa0100730:intron 35.0%
AAGCATGCTAAAATGTTGTC+TGG - chr1_3:7165488-7165507 Msa0100730:intron 35.0%
ATTTATCCCACATCATCATC+TGG - chr1_3:7165546-7165565 Msa0100730:intron 35.0%
ATAAGAGACTTGTGGATATG+AGG + chr1_3:7165855-7165874 None:intergenic 35.0%
! ACTTGTTGATAAGAGACTTG+TGG + chr1_3:7165863-7165882 None:intergenic 35.0%
TCTTATCAACAAGTCCTCAA+AGG - chr1_3:7165869-7165888 Msa0100730:intron 35.0%
!! ACATCTTCACTTTTGTGGTA+TGG - chr1_3:7166072-7166091 Msa0100730:CDS 35.0%
AACATAAATCCTAGACATGC+AGG + chr1_3:7166100-7166119 None:intergenic 35.0%
! TGCATGTCTAGGATTTATGT+TGG - chr1_3:7166099-7166118 Msa0100730:CDS 35.0%
!! TATGTCTGTTTTGAACTGCT+TGG - chr1_3:7166140-7166159 Msa0100730:five_prime_UTR 35.0%
!!! AGAGAGAGATTTGGCTTTTG+AGG + chr1_3:7162732-7162751 None:intergenic 40.0%
TTGAAGTGCGAAAACATGTC+GGG - chr1_3:7162782-7162801 Msa0100730:three_prime_UTR 40.0%
TCTTCTTGTCCTTGTCTTTG+AGG + chr1_3:7162850-7162869 None:intergenic 40.0%
GATGAAGAGAAAGGGTAAGA+GGG + chr1_3:7162922-7162941 None:intergenic 40.0%
AGATGAAGTATGGGGTAGAA+AGG - chr1_3:7163159-7163178 Msa0100730:CDS 40.0%
TTCCAAAGACACCATCAATG+TGG + chr1_3:7163328-7163347 None:intergenic 40.0%
!! ACATTGATGGTGTCTTTGGA+AGG - chr1_3:7163327-7163346 Msa0100730:intron 40.0%
!! CATTGATGGTGTCTTTGGAA+GGG - chr1_3:7163328-7163347 Msa0100730:intron 40.0%
!! ATTGATGGTGTCTTTGGAAG+GGG - chr1_3:7163329-7163348 Msa0100730:intron 40.0%
TTGGACTTCTAACAGAGACT+AGG - chr1_3:7163618-7163637 Msa0100730:intron 40.0%
CATATGTTCTCTGTGGTCAT+GGG - chr1_3:7163787-7163806 Msa0100730:intron 40.0%
! CTCGCGCATTTTCTTTTTTC+GGG + chr1_3:7163898-7163917 None:intergenic 40.0%
GAAAAAAGAAAATGCGCGAG+TGG - chr1_3:7163898-7163917 Msa0100730:intron 40.0%
ATCCCTTCTAATATCCACCA+AGG + chr1_3:7163924-7163943 None:intergenic 40.0%
TATTAGCTAACTATGCGGTG+TGG + chr1_3:7164153-7164172 None:intergenic 40.0%
ACACCGCATAGTTAGCTAAT+AGG - chr1_3:7164152-7164171 Msa0100730:intron 40.0%
CTGCCTATTAGCTAACTATG+CGG + chr1_3:7164158-7164177 None:intergenic 40.0%
ATGACAGTGTGTGCTATCAA+GGG + chr1_3:7164258-7164277 None:intergenic 40.0%
TATGACAGTGTGTGCTATCA+AGG + chr1_3:7164259-7164278 None:intergenic 40.0%
CTTGACTGCAACGTAAAAGA+TGG + chr1_3:7164362-7164381 None:intergenic 40.0%
ATGCTTCACTCTCAAACCAA+TGG - chr1_3:7164394-7164413 Msa0100730:intron 40.0%
AGAATCATATGTGAATGGGG+TGG - chr1_3:7164454-7164473 Msa0100730:intron 40.0%
! TTTAAAACAGGTTACGGGTG+AGG - chr1_3:7164621-7164640 Msa0100730:intron 40.0%
! CATAATCTTTTGTGGTCCAG+TGG - chr1_3:7164837-7164856 Msa0100730:intron 40.0%
CCAAAAAAGCAGATTACCAC+TGG + chr1_3:7164856-7164875 None:intergenic 40.0%
!!! CCAGTGGTAATCTGCTTTTT+TGG - chr1_3:7164853-7164872 Msa0100730:intron 40.0%
!!! CTGCTTTTTTGGTTCCTATG+AGG - chr1_3:7164864-7164883 Msa0100730:intron 40.0%
AAAACATGCAACTCTTACCG+TGG - chr1_3:7164981-7165000 Msa0100730:intron 40.0%
!! AGCTTGGATGGTTTTTCAGA+GGG + chr1_3:7165063-7165082 None:intergenic 40.0%
! TAATGTCAGGCTGCACTTTT+TGG + chr1_3:7165280-7165299 None:intergenic 40.0%
AAAGTGCAGCCTGACATTAA+AGG - chr1_3:7165281-7165300 Msa0100730:intron 40.0%
! GGAAAACCAGATGATGATGT+GGG + chr1_3:7165555-7165574 None:intergenic 40.0%
! TGGAAAACCAGATGATGATG+TGG + chr1_3:7165556-7165575 None:intergenic 40.0%
TTCTACATACAGTTCCCTGT+CGG - chr1_3:7165588-7165607 Msa0100730:intron 40.0%
!! TCTTCTGAAGTCAAGGACAA+TGG - chr1_3:7165623-7165642 Msa0100730:intron 40.0%
CCTCTGCAGTTAAATACTCT+AGG + chr1_3:7165695-7165714 None:intergenic 40.0%
! CCTAGAGTATTTAACTGCAG+AGG - chr1_3:7165692-7165711 Msa0100730:intron 40.0%
! GTATTTAACTGCAGAGGTGT+TGG - chr1_3:7165698-7165717 Msa0100730:intron 40.0%
ATAGCAAGCTGTAGATGTCT+CGG + chr1_3:7165771-7165790 None:intergenic 40.0%
GAACTTGACACTCTCATCAA+AGG - chr1_3:7165804-7165823 Msa0100730:intron 40.0%
CTCATCAAAGGAACAATAGC+TGG - chr1_3:7165816-7165835 Msa0100730:intron 40.0%
ATCAAAGGAACAATAGCTGG+TGG - chr1_3:7165819-7165838 Msa0100730:intron 40.0%
CAATCAATCAAACCAACGCA+AGG + chr1_3:7165991-7166010 None:intergenic 40.0%
!! TGTTTTGAACTGCTTGGCAA+TGG - chr1_3:7166146-7166165 Msa0100730:five_prime_UTR 40.0%
! AATGGAAAGTCTTTTGCTGC+CGG - chr1_3:7166172-7166191 Msa0100730:five_prime_UTR 40.0%
GAGAGAGAGAGAGAGAGATT+TGG + chr1_3:7162741-7162760 None:intergenic 45.0%
TGAAGTGCGAAAACATGTCG+GGG - chr1_3:7162783-7162802 Msa0100730:three_prime_UTR 45.0%
!!! GAGGTTTGCTGGTGTTTTAC+CGG + chr1_3:7162831-7162850 None:intergenic 45.0%
ACCAGCAAACCTCAAAGACA+AGG - chr1_3:7162838-7162857 Msa0100730:three_prime_UTR 45.0%
!! TCCTTGTCTTTGAGGTTTGC+TGG + chr1_3:7162842-7162861 None:intergenic 45.0%
CGATGAAGAGAAAGGGTAAG+AGG + chr1_3:7162923-7162942 None:intergenic 45.0%
!! TGCCACATTGATGGTGTCTT+TGG - chr1_3:7163323-7163342 Msa0100730:intron 45.0%
GTTTCATGGCGAATGATACG+CGG - chr1_3:7163407-7163426 Msa0100730:intron 45.0%
GCATATGTTCTCTGTGGTCA+TGG - chr1_3:7163786-7163805 Msa0100730:intron 45.0%
! TGGCCTTGGTGGATATTAGA+AGG - chr1_3:7163918-7163937 Msa0100730:intron 45.0%
! GGCCTTGGTGGATATTAGAA+GGG - chr1_3:7163919-7163938 Msa0100730:intron 45.0%
GGGAAATAATAGTTCCGGGA+GGG + chr1_3:7163962-7163981 None:intergenic 45.0%
AGGGAAATAATAGTTCCGGG+AGG + chr1_3:7163963-7163982 None:intergenic 45.0%
ATTCGGTTTAGCGCATTCGT+GGG + chr1_3:7164036-7164055 None:intergenic 45.0%
! AAGCGGCGAGTTGTTGATAA+TGG + chr1_3:7164210-7164229 None:intergenic 45.0%
TCAATATCGAAGTGGTTGGG+TGG - chr1_3:7164321-7164340 Msa0100730:intron 45.0%
CAATATCGAAGTGGTTGGGT+GGG - chr1_3:7164322-7164341 Msa0100730:intron 45.0%
AATATCGAAGTGGTTGGGTG+GGG - chr1_3:7164323-7164342 Msa0100730:intron 45.0%
GTGTGTGCAGCATCATTTGT+GGG - chr1_3:7164722-7164741 Msa0100730:intron 45.0%
ACTCGAGCTTCTATCCTCAT+AGG + chr1_3:7164881-7164900 None:intergenic 45.0%
TGTTGATGTGAGTCGAACCT+TGG - chr1_3:7164947-7164966 Msa0100730:intron 45.0%
!! CTTGGATGGTTTTTCAGAGG+GGG + chr1_3:7165061-7165080 None:intergenic 45.0%
!! GCTTGGATGGTTTTTCAGAG+GGG + chr1_3:7165062-7165081 None:intergenic 45.0%
!! GAGCTTGGATGGTTTTTCAG+AGG + chr1_3:7165064-7165083 None:intergenic 45.0%
CACATTGCTCACCTTATCAG+TGG + chr1_3:7165089-7165108 None:intergenic 45.0%
AAGCTGCAGCCTTTAATGTC+AGG + chr1_3:7165293-7165312 None:intergenic 45.0%
ACATTAAAGGCTGCAGCTTC+TGG - chr1_3:7165294-7165313 Msa0100730:intron 45.0%
TGCAGCTTCTGGTAAGTCAT+AGG - chr1_3:7165305-7165324 Msa0100730:intron 45.0%
CCAAAGATACGTCACTCACA+TGG + chr1_3:7165371-7165390 None:intergenic 45.0%
CCATGTGAGTGACGTATCTT+TGG - chr1_3:7165368-7165387 Msa0100730:intron 45.0%
!! TCCATCGTCTTCTGAAGTCA+AGG - chr1_3:7165616-7165635 Msa0100730:intron 45.0%
TCCTTGACTTCAGAAGACGA+TGG + chr1_3:7165620-7165639 None:intergenic 45.0%
AAGTCAAGGACAATGGCACA+TGG - chr1_3:7165630-7165649 Msa0100730:intron 45.0%
ACAATGGCACATGGAAGAGT+TGG - chr1_3:7165639-7165658 Msa0100730:intron 45.0%
CAATGGCACATGGAAGAGTT+GGG - chr1_3:7165640-7165659 Msa0100730:intron 45.0%
AATGGCACATGGAAGAGTTG+GGG - chr1_3:7165641-7165660 Msa0100730:intron 45.0%
CAATGCAAGCAAGGATCTGA+AGG - chr1_3:7165731-7165750 Msa0100730:intron 45.0%
TGTCAAGTTCTTCGTCTCCA+CGG + chr1_3:7165794-7165813 None:intergenic 45.0%
AAAGGAACAATAGCTGGTGG+TGG - chr1_3:7165822-7165841 Msa0100730:intron 45.0%
ACTGCTTGGCAATGGAGTAA+TGG - chr1_3:7166154-7166173 Msa0100730:five_prime_UTR 45.0%
TGCGAAAACATGTCGGGGAA+AGG - chr1_3:7162788-7162807 Msa0100730:three_prime_UTR 50.0%
GCTCTTGAGGAACGAGATGT+GGG + chr1_3:7162875-7162894 None:intergenic 50.0%
AGCTCTTGAGGAACGAGATG+TGG + chr1_3:7162876-7162895 None:intergenic 50.0%
ACATCTCGTTCCTCAAGAGC+TGG - chr1_3:7162875-7162894 Msa0100730:three_prime_UTR 50.0%
!! TTCCTCAAGAGCTGGTCTTC+AGG - chr1_3:7162883-7162902 Msa0100730:three_prime_UTR 50.0%
AACCTGAAGACCAGCTCTTG+AGG + chr1_3:7162888-7162907 None:intergenic 50.0%
!! ACATGTGTCGGTGTTGTGTC+AGG - chr1_3:7163491-7163510 Msa0100730:intron 50.0%
!! CATGTGTCGGTGTTGTGTCA+GGG - chr1_3:7163492-7163511 Msa0100730:intron 50.0%
TAATAGTTCCGGGAGGGCTA+TGG + chr1_3:7163956-7163975 None:intergenic 50.0%
TTCGGTTTAGCGCATTCGTG+GGG + chr1_3:7164035-7164054 None:intergenic 50.0%
CATTCGGTTTAGCGCATTCG+TGG + chr1_3:7164037-7164056 None:intergenic 50.0%
CACGAATGCGCTAAACCGAA+TGG - chr1_3:7164035-7164054 Msa0100730:intron 50.0%
!! CGGCGAGTTGTTGATAATGG+TGG + chr1_3:7164207-7164226 None:intergenic 50.0%
CAGTGTGTGCTATCAAGGGA+TGG + chr1_3:7164254-7164273 None:intergenic 50.0%
ATATCGAAGTGGTTGGGTGG+GGG - chr1_3:7164324-7164343 Msa0100730:intron 50.0%
!! GTGAATGGGGTGGATTAGTG+CGG - chr1_3:7164464-7164483 Msa0100730:intron 50.0%
!! TGAATGGGGTGGATTAGTGC+GGG - chr1_3:7164465-7164484 Msa0100730:intron 50.0%
!! TACGGGTGAGGTTCTAAGAG+AGG - chr1_3:7164633-7164652 Msa0100730:intron 50.0%
GGTGTGTGCAGCATCATTTG+TGG - chr1_3:7164721-7164740 Msa0100730:intron 50.0%
TATCAGTGGCAGAGCTTGGA+TGG + chr1_3:7165075-7165094 None:intergenic 50.0%
TCCAAGCTCTGCCACTGATA+AGG - chr1_3:7165075-7165094 Msa0100730:intron 50.0%
ACCTTATCAGTGGCAGAGCT+TGG + chr1_3:7165079-7165098 None:intergenic 50.0%
GGCTGTAGTCGTAACACATG+TGG + chr1_3:7165350-7165369 None:intergenic 50.0%
ACATGGAAGAGTTGGGGCTA+CGG - chr1_3:7165647-7165666 Msa0100730:intron 50.0%
!! AACTGCAGAGGTGTTGGAGT+TGG - chr1_3:7165704-7165723 Msa0100730:intron 50.0%
!! CATCTACAGCTTGCTATCCG+TGG - chr1_3:7165774-7165793 Msa0100730:intron 50.0%
GGATAATTCGCTCCTTGCGT+TGG - chr1_3:7165976-7165995 Msa0100730:intron 50.0%
GGTATGGTACCTGCATGTCT+AGG - chr1_3:7166088-7166107 Msa0100730:CDS 50.0%
ATGTCGGGGAAAGGAGCGAA+GGG - chr1_3:7162797-7162816 Msa0100730:three_prime_UTR 55.0%
GGAGCGAAGGGTTTGACAAC+CGG - chr1_3:7162809-7162828 Msa0100730:three_prime_UTR 55.0%
!! TGTCGGTGTTGTGTCAGGGT+TGG - chr1_3:7163496-7163515 Msa0100730:intron 55.0%
AGAAAATGCGCGAGTGGCCT+TGG - chr1_3:7163904-7163923 Msa0100730:intron 55.0%
TCGGTTTAGCGCATTCGTGG+GGG + chr1_3:7164034-7164053 None:intergenic 55.0%
GACGATGGATACGTCCGACA+GGG + chr1_3:7165605-7165624 None:intergenic 55.0%
AGACGATGGATACGTCCGAC+AGG + chr1_3:7165606-7165625 None:intergenic 55.0%
GTTGGCTGGCAATGCAAGCA+AGG - chr1_3:7165722-7165741 Msa0100730:intron 55.0%
CATGTCGGGGAAAGGAGCGA+AGG - chr1_3:7162796-7162815 Msa0100730:three_prime_UTR 60.0%
! AAATGCGCGAGTGGCCTTGG+TGG - chr1_3:7163907-7163926 Msa0100730:intron 60.0%
!! GCAGAGGTGTTGGAGTTGGC+TGG - chr1_3:7165708-7165727 Msa0100730:intron 60.0%
TAGGGTGGCCATAGCCCTCC+CGG - chr1_3:7163945-7163964 Msa0100730:intron 65.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 7162723 7166202 7162723 ID=Msa0100730;Name=Msa0100730
chr1_3 mRNA 7162723 7166202 7162723 ID=Msa0100730-mRNA-1;Parent=Msa0100730;Name=Msa0100730-mRNA-1;_AED=0.26;_eAED=0.26;_QI=74|1|1|1|1|1|2|306|134
chr1_3 exon 7162723 7163325 7162723 ID=Msa0100730-mRNA-1:exon:2795;Parent=Msa0100730-mRNA-1
chr1_3 exon 7166021 7166202 7166021 ID=Msa0100730-mRNA-1:exon:2794;Parent=Msa0100730-mRNA-1
chr1_3 five_prime_UTR 7166129 7166202 7166129 ID=Msa0100730-mRNA-1:five_prime_utr;Parent=Msa0100730-mRNA-1
chr1_3 CDS 7166021 7166128 7166021 ID=Msa0100730-mRNA-1:cds;Parent=Msa0100730-mRNA-1
chr1_3 CDS 7163029 7163325 7163029 ID=Msa0100730-mRNA-1:cds;Parent=Msa0100730-mRNA-1
chr1_3 three_prime_UTR 7162723 7163028 7162723 ID=Msa0100730-mRNA-1:three_prime_utr;Parent=Msa0100730-mRNA-1
Gene Sequence

>Msa0100730

ATGTCGGGGAAAGGAGCGAAGGGTTTGACAACCGGTAAAACACCAGCAAACCTCAAAGACAAGGACAAGAAGAAACCCACATCTCGTTCCTCAAGAGCTGGTCTTCAGTTCCCTGTCGGACGTATCCATCGTCTTCTGAAGTCAAGGACAATGGCACATGGAAGAGTTGGGGCTACGGCTGCAGTTTATTCTGCCGCTATCCTAGAGTATTTAACTGCAGAGGTGTTGGAGTTGGCTGGCAATGCAAGCAAGGATCTGAAGGTGAAGCGTATTACACCGAGACATCTACAGCTTGCTATCCGTGGAGACGAAGAACTTGACACTCTCATCAAAGGAACAATAGCTGGTGGTGGTGTTATTCCTCATATCCACAAGTCTCTTATCAACAAGTCCTCAAAGGAATGA
Protein sequence

>Msa0100730

MSGKGAKGLTTGKTPANLKDKDKKKPTSRSSRAGLQFPVGRIHRLLKSRTMAHGRVGATAAVYSAAILEYLTAEVLELAGNASKDLKVKRITPRHLQLAIRGDEELDTLIKGTIAGGGVIPHIHKSLINKSSKE*