Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0026910 | Msa1168690 | 0.803633 | 3.176800e-49 | -8.615850e-47 |
| Msa0773310 | Msa1168690 | 0.800799 | 1.216244e-48 | -8.615850e-47 |
| Msa0197660 | Msa1168690 | 0.820024 | 8.531690e-53 | -8.615850e-47 |
| Msa0239800 | Msa1168690 | 0.813848 | 2.079305e-51 | -8.615850e-47 |
| Msa0317510 | Msa1168690 | 0.806929 | 6.477623e-50 | -8.615850e-47 |
| Msa1168690 | Msa1307550 | 0.801771 | 7.695616e-49 | -8.615850e-47 |
| Msa0518380 | Msa1168690 | 0.822037 | 2.934098e-53 | -8.615850e-47 |
| Msa0562440 | Msa1168690 | 0.801216 | 9.995312e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 20 sgRNAs with CRISPR-Local
Find 41 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACCGTGTGCAGCACGAAATC+TGG | 0.330125 | 7_4:-61472398 | None:intergenic |
| GAAATTATCACTAATCTATC+AGG | 0.375938 | 7_4:+61472433 | Msa1168690:CDS |
| TGAGCTTGTGGTGGAGGAGC+TGG | 0.378215 | 7_4:-61472858 | None:intergenic |
| AGCTTTAGCTTGTTACCTGT+TGG | 0.405704 | 7_4:+61472782 | Msa1168690:intron |
| CACGAAATCTGGTGAGGGAA+TGG | 0.427773 | 7_4:-61472387 | None:intergenic |
| GCTTTAGCTTGTTACCTGTT+GGG | 0.433665 | 7_4:+61472783 | Msa1168690:intron |
| ATTAGTGATAATTTCAATGA+AGG | 0.433856 | 7_4:-61472425 | None:intergenic |
| TCTTCCCATCCACAGCAGTA+TGG | 0.446501 | 7_4:+61472831 | Msa1168690:CDS |
| GTGGGCCATACTGCTGTGGA+TGG | 0.449759 | 7_4:-61472836 | None:intergenic |
| GCTTGTGGTGGAGGAGCTGG+TGG | 0.476856 | 7_4:-61472855 | None:intergenic |
| TATGGTTGAGCTTGTGGTGG+AGG | 0.477908 | 7_4:-61472864 | None:intergenic |
| ACGAAATCTGGTGAGGGAAT+GGG | 0.486822 | 7_4:-61472386 | None:intergenic |
| ACCAGATTTCGTGCTGCACA+CGG | 0.568645 | 7_4:+61472397 | Msa1168690:CDS |
| TGGGCCATACTGCTGTGGAT+GGG | 0.572859 | 7_4:-61472835 | None:intergenic |
| CTTGTGGTGGAGGAGCTGGT+GGG | 0.578321 | 7_4:-61472854 | None:intergenic |
| TGTTATGGTTGAGCTTGTGG+TGG | 0.599848 | 7_4:-61472867 | None:intergenic |
| TGCAGCACGAAATCTGGTGA+GGG | 0.604754 | 7_4:-61472392 | None:intergenic |
| GCTGGTGGGCCATACTGCTG+TGG | 0.609985 | 7_4:-61472840 | None:intergenic |
| GTGCAGCACGAAATCTGGTG+AGG | 0.633765 | 7_4:-61472393 | None:intergenic |
| TACCTGTTGGGCAAGTACAG+AGG | 0.661863 | 7_4:+61472795 | Msa1168690:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATGATTTTTTATGACAAATT+GGG | - | chr7_4:61472483-61472502 | None:intergenic | 15.0% |
| !! | AAATAAATAAAGGAAGAAAA+AGG | + | chr7_4:61472695-61472714 | Msa1168690:intron | 15.0% |
| !!! | GTAGTTTTAGAAGAAAATAA+AGG | - | chr7_4:61472323-61472342 | None:intergenic | 20.0% |
| !! | ATTAGTGATAATTTCAATGA+AGG | - | chr7_4:61472428-61472447 | None:intergenic | 20.0% |
| !!! | GATGATTTTTTATGACAAAT+TGG | - | chr7_4:61472484-61472503 | None:intergenic | 20.0% |
| !!! | TTGAAATATGTTTTTGGAAA+TGG | - | chr7_4:61472537-61472556 | None:intergenic | 20.0% |
| !!! | ATGGAATTGAAATATGTTTT+TGG | - | chr7_4:61472543-61472562 | None:intergenic | 20.0% |
| !! | AATATTTCATACTAGATTGA+TGG | - | chr7_4:61472562-61472581 | None:intergenic | 20.0% |
| !! | GCTTAGGTATAAATAAATAA+AGG | + | chr7_4:61472685-61472704 | Msa1168690:intron | 20.0% |
| !! | ATCAAGATGAACAATATTAA+TGG | - | chr7_4:61472759-61472778 | None:intergenic | 20.0% |
| !!! | GTTTTAGAAGAAAATAAAGG+TGG | - | chr7_4:61472320-61472339 | None:intergenic | 25.0% |
| ! | GAAATTATCACTAATCTATC+AGG | + | chr7_4:61472433-61472452 | Msa1168690:CDS | 25.0% |
| ! | TTATGACAAATTGGGAAAAA+AGG | - | chr7_4:61472475-61472494 | None:intergenic | 25.0% |
| ! | AAAAAGGAAAGTTAGAGAAT+GGG | + | chr7_4:61472711-61472730 | Msa1168690:intron | 25.0% |
| ! | ATTGATGAAGATTTCTGAGA+GGG | - | chr7_4:61472508-61472527 | None:intergenic | 30.0% |
| AATTGATGAAGATTTCTGAG+AGG | - | chr7_4:61472509-61472528 | None:intergenic | 30.0% | |
| TCTAAGAATTGAAGATGACA+TGG | + | chr7_4:61472663-61472682 | Msa1168690:intron | 30.0% | |
| GAAAAAGGAAAGTTAGAGAA+TGG | + | chr7_4:61472710-61472729 | Msa1168690:intron | 30.0% | |
| AGACAAGATGAGATCTCATT+AGG | - | chr7_4:61472294-61472313 | None:intergenic | 35.0% | |
| ! | TTGATGAAGATTTCTGAGAG+GGG | - | chr7_4:61472507-61472526 | None:intergenic | 35.0% |
| AATTGAAGATGACATGGCTT+AGG | + | chr7_4:61472669-61472688 | Msa1168690:intron | 35.0% | |
| ! | TGATGAAGATTTCTGAGAGG+GGG | - | chr7_4:61472506-61472525 | None:intergenic | 40.0% |
| TTAGAGAATGGGATTCGAGT+TGG | + | chr7_4:61472722-61472741 | Msa1168690:intron | 40.0% | |
| !! | AGCTTTAGCTTGTTACCTGT+TGG | + | chr7_4:61472782-61472801 | Msa1168690:intron | 40.0% |
| !! | GCTTTAGCTTGTTACCTGTT+GGG | + | chr7_4:61472783-61472802 | Msa1168690:intron | 40.0% |
| ACGAAATCTGGTGAGGGAAT+GGG | - | chr7_4:61472389-61472408 | None:intergenic | 45.0% | |
| CACGAAATCTGGTGAGGGAA+TGG | - | chr7_4:61472390-61472409 | None:intergenic | 50.0% | |
| TGCAGCACGAAATCTGGTGA+GGG | - | chr7_4:61472395-61472414 | None:intergenic | 50.0% | |
| ACCAGATTTCGTGCTGCACA+CGG | + | chr7_4:61472397-61472416 | Msa1168690:CDS | 50.0% | |
| TACCTGTTGGGCAAGTACAG+AGG | + | chr7_4:61472795-61472814 | Msa1168690:CDS | 50.0% | |
| TCTTCCCATCCACAGCAGTA+TGG | + | chr7_4:61472831-61472850 | Msa1168690:CDS | 50.0% | |
| TATGGTTGAGCTTGTGGTGG+AGG | - | chr7_4:61472867-61472886 | None:intergenic | 50.0% | |
| GTGCAGCACGAAATCTGGTG+AGG | - | chr7_4:61472396-61472415 | None:intergenic | 55.0% | |
| ACCGTGTGCAGCACGAAATC+TGG | - | chr7_4:61472401-61472420 | None:intergenic | 55.0% | |
| GACCTCTGTACTTGCCCAAC+AGG | - | chr7_4:61472800-61472819 | None:intergenic | 55.0% | |
| TGGGCCATACTGCTGTGGAT+GGG | - | chr7_4:61472838-61472857 | None:intergenic | 55.0% | |
| GTGGGCCATACTGCTGTGGA+TGG | - | chr7_4:61472839-61472858 | None:intergenic | 60.0% | |
| ! | CTTGTGGTGGAGGAGCTGGT+GGG | - | chr7_4:61472857-61472876 | None:intergenic | 60.0% |
| ! | TGAGCTTGTGGTGGAGGAGC+TGG | - | chr7_4:61472861-61472880 | None:intergenic | 60.0% |
| ! | GCTGGTGGGCCATACTGCTG+TGG | - | chr7_4:61472843-61472862 | None:intergenic | 65.0% |
| ! | GCTTGTGGTGGAGGAGCTGG+TGG | - | chr7_4:61472858-61472877 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_4 | gene | 61472260 | 61472887 | 61472260 | ID=Msa1168690;Name=Msa1168690 |
| chr7_4 | mRNA | 61472260 | 61472887 | 61472260 | ID=Msa1168690-mRNA-1;Parent=Msa1168690;Name=Msa1168690-mRNA-1;_AED=0.30;_eAED=0.67;_QI=0|0|0|0.66|0|0|3|0|63 |
| chr7_4 | exon | 61472260 | 61472265 | 61472260 | ID=Msa1168690-mRNA-1:exon:19439;Parent=Msa1168690-mRNA-1 |
| chr7_4 | exon | 61472367 | 61472454 | 61472367 | ID=Msa1168690-mRNA-1:exon:19440;Parent=Msa1168690-mRNA-1 |
| chr7_4 | exon | 61472790 | 61472887 | 61472790 | ID=Msa1168690-mRNA-1:exon:19441;Parent=Msa1168690-mRNA-1 |
| chr7_4 | CDS | 61472260 | 61472265 | 61472260 | ID=Msa1168690-mRNA-1:cds;Parent=Msa1168690-mRNA-1 |
| chr7_4 | CDS | 61472367 | 61472454 | 61472367 | ID=Msa1168690-mRNA-1:cds;Parent=Msa1168690-mRNA-1 |
| chr7_4 | CDS | 61472790 | 61472887 | 61472790 | ID=Msa1168690-mRNA-1:cds;Parent=Msa1168690-mRNA-1 |
| Gene Sequence |
| Protein sequence |
