AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1171330

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1171330 MtrunA17_Chr7g0266481 98.113 106 2 0 1 106 1 106 1.35e-74 216
Msa1171330 MtrunA17_Chr1g0210621 54.206 107 48 1 1 106 1 107 2.05e-38 124
Msa1171330 MtrunA17_Chr1g0210651 55.140 107 47 1 1 106 1 107 7.15e-38 123
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1171330 AT4G18590.1 58.491 106 44 0 1 106 1 106 2.75e-46 144
Msa1171330 AT3G52630.2 55.140 107 47 1 1 106 1 107 7.74e-42 133
Msa1171330 AT3G52630.1 55.140 107 47 1 1 106 1 107 7.74e-42 133

Find 42 sgRNAs with CRISPR-Local

Find 197 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACCCTTCTTCCGACATAAA+AGG 0.241109 8_1:+945164 None:intergenic
TCTGTGAACTATCATCTAAA+AGG 0.263776 8_1:+944359 None:intergenic
ACAAGGACTGCAGGGCTTAA+TGG 0.275580 8_1:+945204 None:intergenic
GAGAAGCAGTTGATTGTAAA+AGG 0.288767 8_1:-945081 Msa1171330:CDS
ATGCTGATCACTGCATTAAA+AGG 0.296057 8_1:+944410 None:intergenic
ACCAACTTTGGTGATGAAAT+TGG 0.344480 8_1:-944976 Msa1171330:intron
TTGCAAGCTGACATAGCTTA+TGG 0.355631 8_1:+944770 None:intergenic
GGTCCATAACTCAGCTTTGA+TGG 0.369681 8_1:+944996 None:intergenic
AGGAGTACAGTAGAAGGTTC+TGG 0.374761 8_1:-946174 Msa1171330:five_prime_UTR
ACAATCAACTGCTTCTCATC+TGG 0.376539 8_1:+945087 None:intergenic
CTGTGAACTATCATCTAAAA+GGG 0.380041 8_1:+944360 None:intergenic
GAAGGTTCTGGAAAGGAAAA+AGG 0.382096 8_1:-946162 Msa1171330:five_prime_UTR
ATCTGCAAATTGAATCAATC+TGG 0.421426 8_1:-946070 Msa1171330:intron
ACCAATTTCATCACCAAAGT+TGG 0.430140 8_1:+944975 None:intergenic
ACAAAGACATCTCTATCATA+TGG 0.434457 8_1:-944689 Msa1171330:three_prime_UTR
ATGTTGAGAAATAGATTTGT+TGG 0.434718 8_1:-944465 Msa1171330:three_prime_UTR
ACAAAACTTGTAAGAGGAGC+AGG 0.439485 8_1:+945051 None:intergenic
ACTTCTACAAAACTTGTAAG+AGG 0.459678 8_1:+945045 None:intergenic
GCTGAGTTATGGACCAACTT+TGG 0.463853 8_1:-944988 Msa1171330:CDS
TCTGCATTGACAAGGACTGC+AGG 0.468536 8_1:+945195 None:intergenic
ACCCTTCTTCCGACATAAAA+GGG 0.468915 8_1:+945165 None:intergenic
AAGCTATGTCAGCTTGCAAA+TGG 0.472567 8_1:-944766 Msa1171330:CDS
TCTGACGCTCTAGCTGTCAT+TGG 0.479697 8_1:-945117 Msa1171330:CDS
GACCAGAGGAGTACAGTAGA+AGG 0.493178 8_1:-946180 Msa1171330:five_prime_UTR
AGTCCATCAAAGCTGAGTTA+TGG 0.498331 8_1:-944999 Msa1171330:CDS
AACCTTCTACTGTACTCCTC+TGG 0.508205 8_1:+946178 None:intergenic
AAGGGTTCGTACAGTGATGC+AGG 0.527340 8_1:-945148 Msa1171330:CDS
AGGAGACATTACTGTTGTGT+TGG 0.530242 8_1:-944657 Msa1171330:three_prime_UTR
CAAGCAGATATTATTTACCA+TGG 0.547450 8_1:+944605 None:intergenic
CTGCATTGACAAGGACTGCA+GGG 0.550141 8_1:+945196 None:intergenic
AAGCAGATATTATTTACCAT+GGG 0.598106 8_1:+944606 None:intergenic
ATAAATAGAGGGAGGACCAG+AGG 0.608531 8_1:-946194 None:intergenic
CAAAACTTGTAAGAGGAGCA+GGG 0.615820 8_1:+945052 None:intergenic
TACAGTAGAAGGTTCTGGAA+AGG 0.618140 8_1:-946169 Msa1171330:five_prime_UTR
CTATCATATGGAATAACTAG+AGG 0.627927 8_1:-944677 Msa1171330:three_prime_UTR
GAGACGAGAGAGTAGAGCGA+GGG 0.635654 8_1:+946133 None:intergenic
AAAACTTGTAAGAGGAGCAG+GGG 0.643901 8_1:+945053 None:intergenic
CTTGCTGTAACTCAACTCAC+AGG 0.644780 8_1:+944725 None:intergenic
TGTGAACTATCATCTAAAAG+GGG 0.646431 8_1:+944361 None:intergenic
GCAACAGTTCTGCATTGACA+AGG 0.657999 8_1:+945187 None:intergenic
CGAGACGAGAGAGTAGAGCG+AGG 0.669211 8_1:+946132 None:intergenic
AAACTTGTAAGAGGAGCAGG+GGG 0.714728 8_1:+945054 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATTTAAACAATTTAAACA+AGG + chr8_1:944774-944793 None:intergenic 10.0%
!! ATTAAAATTTAAATGAAATG+TGG - chr8_1:944790-944809 Msa1171330:intron 10.0%
!!! TGAAATCTATAAAATTTTTT+CGG - chr8_1:945961-945980 Msa1171330:intron 10.0%
!! TAATTTAAACAATTTAAACA+AGG + chr8_1:944774-944793 None:intergenic 10.0%
!! ATTAAAATTTAAATGAAATG+TGG - chr8_1:944790-944809 Msa1171330:intron 10.0%
!!! TGAAATCTATAAAATTTTTT+CGG - chr8_1:945961-945980 Msa1171330:intron 10.0%
!!! GAAATCTATAAAATTTTTTC+GGG - chr8_1:945962-945981 Msa1171330:intron 15.0%
!!! AAATCTATAAAATTTTTTCG+GGG - chr8_1:945963-945982 Msa1171330:intron 15.0%
!!! GAAATCTATAAAATTTTTTC+GGG - chr8_1:945962-945981 Msa1171330:intron 15.0%
!!! AAATCTATAAAATTTTTTCG+GGG - chr8_1:945963-945982 Msa1171330:intron 15.0%
!!! CAATTTTCTCAAAATACTAT+CGG + chr8_1:944882-944901 None:intergenic 20.0%
!!! GTTAGTTCTTTTGTATATTT+TGG - chr8_1:945029-945048 Msa1171330:CDS 20.0%
!!! AGTGTTTTCCTATAATTTTT+AGG + chr8_1:945079-945098 None:intergenic 20.0%
!! GTAATGATATTTATTCTCTA+TGG - chr8_1:945651-945670 Msa1171330:intron 20.0%
!! TTGAAATTTCACTTATAACA+AGG + chr8_1:945678-945697 None:intergenic 20.0%
!!! CAATTTTCTCAAAATACTAT+CGG + chr8_1:944882-944901 None:intergenic 20.0%
!!! GTTAGTTCTTTTGTATATTT+TGG - chr8_1:945029-945048 Msa1171330:CDS 20.0%
!!! AGTGTTTTCCTATAATTTTT+AGG + chr8_1:945079-945098 None:intergenic 20.0%
!! GTAATGATATTTATTCTCTA+TGG - chr8_1:945651-945670 Msa1171330:intron 20.0%
!! TTGAAATTTCACTTATAACA+AGG + chr8_1:945678-945697 None:intergenic 20.0%
! CAATCATGAACACAAAATTA+GGG + chr8_1:944626-944645 None:intergenic 25.0%
! TCAATCATGAACACAAAATT+AGG + chr8_1:944627-944646 None:intergenic 25.0%
! TATTTGTAGATTTGACTGAA+GGG - chr8_1:944712-944731 Msa1171330:three_prime_UTR 25.0%
! ACTAACTAGATAGGTATAAA+TGG + chr8_1:945015-945034 None:intergenic 25.0%
! TACAAAAGAACTAACTAGAT+AGG + chr8_1:945024-945043 None:intergenic 25.0%
!!! TTTCCTATAATTTTTAGGAG+CGG + chr8_1:945074-945093 None:intergenic 25.0%
!! CTTAATTTGGAGTCAAAATA+AGG + chr8_1:945276-945295 None:intergenic 25.0%
!!! ACAAGTTTTGTAGAAGTTAT+TGG - chr8_1:945495-945514 Msa1171330:intron 25.0%
!!! ATATTTTGTTTTTGTTCCCA+TGG - chr8_1:945912-945931 Msa1171330:intron 25.0%
! AAGCAGATATTATTTACCAT+GGG + chr8_1:945931-945950 None:intergenic 25.0%
! ATGTTGAGAAATAGATTTGT+TGG - chr8_1:946069-946088 Msa1171330:intron 25.0%
!! ATAGTTCACAGATTGATTTA+TGG - chr8_1:946186-946205 Msa1171330:five_prime_UTR 25.0%
!! TAGTTCACAGATTGATTTAT+GGG - chr8_1:946187-946206 Msa1171330:five_prime_UTR 25.0%
! CAATCATGAACACAAAATTA+GGG + chr8_1:944626-944645 None:intergenic 25.0%
! TCAATCATGAACACAAAATT+AGG + chr8_1:944627-944646 None:intergenic 25.0%
! TATTTGTAGATTTGACTGAA+GGG - chr8_1:944712-944731 Msa1171330:three_prime_UTR 25.0%
! ACTAACTAGATAGGTATAAA+TGG + chr8_1:945015-945034 None:intergenic 25.0%
! TACAAAAGAACTAACTAGAT+AGG + chr8_1:945024-945043 None:intergenic 25.0%
!!! TTTCCTATAATTTTTAGGAG+CGG + chr8_1:945074-945093 None:intergenic 25.0%
!! CTTAATTTGGAGTCAAAATA+AGG + chr8_1:945276-945295 None:intergenic 25.0%
!!! ACAAGTTTTGTAGAAGTTAT+TGG - chr8_1:945495-945514 Msa1171330:intron 25.0%
!!! ATATTTTGTTTTTGTTCCCA+TGG - chr8_1:945912-945931 Msa1171330:intron 25.0%
! AAGCAGATATTATTTACCAT+GGG + chr8_1:945931-945950 None:intergenic 25.0%
! ATGTTGAGAAATAGATTTGT+TGG - chr8_1:946069-946088 Msa1171330:intron 25.0%
ATCTGCAAATTGAATCAATC+TGG - chr8_1:944464-944483 Msa1171330:three_prime_UTR 30.0%
AATGAAACGATGAATTGGAA+AGG + chr8_1:944497-944516 None:intergenic 30.0%
TAGAGAATGAAACGATGAAT+TGG + chr8_1:944502-944521 None:intergenic 30.0%
! GTGTAAAGAAGCATGAAAAA+TGG + chr8_1:944530-944549 None:intergenic 30.0%
ACAGAAAACACAAAAGAACA+CGG + chr8_1:944591-944610 None:intergenic 30.0%
GTATTTGTAGATTTGACTGA+AGG - chr8_1:944711-944730 Msa1171330:three_prime_UTR 30.0%
! AATTACAGTTTTACCGAATC+AGG - chr8_1:945124-945143 Msa1171330:CDS 30.0%
ATTTCAAATGTCTCCTGATT+CGG + chr8_1:945140-945159 None:intergenic 30.0%
GATGCATTATTACATTAAGC+TGG - chr8_1:945184-945203 Msa1171330:CDS 30.0%
!! TAAGGTGAAAAAGGTATTGA+TGG + chr8_1:945258-945277 None:intergenic 30.0%
GAGTCAAAATAAGGTGAAAA+AGG + chr8_1:945267-945286 None:intergenic 30.0%
CACAACAAGATGACTTAATT+TGG + chr8_1:945289-945308 None:intergenic 30.0%
! TGTGCATATTTTGCAGAATA+TGG - chr8_1:945305-945324 Msa1171330:intron 30.0%
ACTTCTACAAAACTTGTAAG+AGG + chr8_1:945492-945511 None:intergenic 30.0%
! TTTATTCCCTTTTCTCAAAC+TGG - chr8_1:945589-945608 Msa1171330:intron 30.0%
TTTCTAAGCATATTAAGCTG+TGG - chr8_1:945699-945718 Msa1171330:intron 30.0%
TTCTAAGCATATTAAGCTGT+GGG - chr8_1:945700-945719 Msa1171330:intron 30.0%
ACAAAGACATCTCTATCATA+TGG - chr8_1:945845-945864 Msa1171330:intron 30.0%
CTATCATATGGAATAACTAG+AGG - chr8_1:945857-945876 Msa1171330:intron 30.0%
CAAGCAGATATTATTTACCA+TGG + chr8_1:945932-945951 None:intergenic 30.0%
TGTGAACTATCATCTAAAAG+GGG + chr8_1:946176-946195 None:intergenic 30.0%
CTGTGAACTATCATCTAAAA+GGG + chr8_1:946177-946196 None:intergenic 30.0%
TCTGTGAACTATCATCTAAA+AGG + chr8_1:946178-946197 None:intergenic 30.0%
! AGTTCACAGATTGATTTATG+GGG - chr8_1:946188-946207 Msa1171330:five_prime_UTR 30.0%
ATCTGCAAATTGAATCAATC+TGG - chr8_1:944464-944483 Msa1171330:three_prime_UTR 30.0%
AATGAAACGATGAATTGGAA+AGG + chr8_1:944497-944516 None:intergenic 30.0%
TAGAGAATGAAACGATGAAT+TGG + chr8_1:944502-944521 None:intergenic 30.0%
! GTGTAAAGAAGCATGAAAAA+TGG + chr8_1:944530-944549 None:intergenic 30.0%
ACAGAAAACACAAAAGAACA+CGG + chr8_1:944591-944610 None:intergenic 30.0%
GTATTTGTAGATTTGACTGA+AGG - chr8_1:944711-944730 Msa1171330:three_prime_UTR 30.0%
! AATTACAGTTTTACCGAATC+AGG - chr8_1:945124-945143 Msa1171330:CDS 30.0%
ATTTCAAATGTCTCCTGATT+CGG + chr8_1:945140-945159 None:intergenic 30.0%
GATGCATTATTACATTAAGC+TGG - chr8_1:945184-945203 Msa1171330:CDS 30.0%
!! TAAGGTGAAAAAGGTATTGA+TGG + chr8_1:945258-945277 None:intergenic 30.0%
GAGTCAAAATAAGGTGAAAA+AGG + chr8_1:945267-945286 None:intergenic 30.0%
CACAACAAGATGACTTAATT+TGG + chr8_1:945289-945308 None:intergenic 30.0%
! TGTGCATATTTTGCAGAATA+TGG - chr8_1:945305-945324 Msa1171330:intron 30.0%
ACTTCTACAAAACTTGTAAG+AGG + chr8_1:945492-945511 None:intergenic 30.0%
! TTTATTCCCTTTTCTCAAAC+TGG - chr8_1:945589-945608 Msa1171330:intron 30.0%
TTTCTAAGCATATTAAGCTG+TGG - chr8_1:945699-945718 Msa1171330:intron 30.0%
TTCTAAGCATATTAAGCTGT+GGG - chr8_1:945700-945719 Msa1171330:intron 30.0%
ACAAAGACATCTCTATCATA+TGG - chr8_1:945845-945864 Msa1171330:intron 30.0%
CTATCATATGGAATAACTAG+AGG - chr8_1:945857-945876 Msa1171330:intron 30.0%
CAAGCAGATATTATTTACCA+TGG + chr8_1:945932-945951 None:intergenic 30.0%
TGTGAACTATCATCTAAAAG+GGG + chr8_1:946176-946195 None:intergenic 30.0%
CTGTGAACTATCATCTAAAA+GGG + chr8_1:946177-946196 None:intergenic 30.0%
TCTGTGAACTATCATCTAAA+AGG + chr8_1:946178-946197 None:intergenic 30.0%
AAATTGCTGAAAAACGAAGC+GGG + chr8_1:944442-944461 None:intergenic 35.0%
CAAATTGCTGAAAAACGAAG+CGG + chr8_1:944443-944462 None:intergenic 35.0%
TTCAAGATACTATCAGACAC+CGG - chr8_1:944942-944961 Msa1171330:intron 35.0%
!! CTATAATTTTTAGGAGCGGA+AGG + chr8_1:945070-945089 None:intergenic 35.0%
CTTCCGCTCCTAAAAATTAT+AGG - chr8_1:945068-945087 Msa1171330:CDS 35.0%
! GAGACATTTGAAATCTGTAC+AGG - chr8_1:945146-945165 Msa1171330:CDS 35.0%
GAGAAGCAGTTGATTGTAAA+AGG - chr8_1:945453-945472 Msa1171330:intron 35.0%
!! ACCAACTTTGGTGATGAAAT+TGG - chr8_1:945558-945577 Msa1171330:intron 35.0%
ACCAATTTCATCACCAAAGT+TGG + chr8_1:945562-945581 None:intergenic 35.0%
TGACAACCAGTTTGAGAAAA+GGG + chr8_1:945598-945617 None:intergenic 35.0%
ATGACAACCAGTTTGAGAAA+AGG + chr8_1:945599-945618 None:intergenic 35.0%
ATGCTGATCACTGCATTAAA+AGG + chr8_1:946127-946146 None:intergenic 35.0%
AAATTGCTGAAAAACGAAGC+GGG + chr8_1:944442-944461 None:intergenic 35.0%
CAAATTGCTGAAAAACGAAG+CGG + chr8_1:944443-944462 None:intergenic 35.0%
TTCAAGATACTATCAGACAC+CGG - chr8_1:944942-944961 Msa1171330:intron 35.0%
!! CTATAATTTTTAGGAGCGGA+AGG + chr8_1:945070-945089 None:intergenic 35.0%
CTTCCGCTCCTAAAAATTAT+AGG - chr8_1:945068-945087 Msa1171330:CDS 35.0%
! GAGACATTTGAAATCTGTAC+AGG - chr8_1:945146-945165 Msa1171330:CDS 35.0%
GAGAAGCAGTTGATTGTAAA+AGG - chr8_1:945453-945472 Msa1171330:intron 35.0%
!! ACCAACTTTGGTGATGAAAT+TGG - chr8_1:945558-945577 Msa1171330:intron 35.0%
ACCAATTTCATCACCAAAGT+TGG + chr8_1:945562-945581 None:intergenic 35.0%
TGACAACCAGTTTGAGAAAA+GGG + chr8_1:945598-945617 None:intergenic 35.0%
ATGACAACCAGTTTGAGAAA+AGG + chr8_1:945599-945618 None:intergenic 35.0%
ATGCTGATCACTGCATTAAA+AGG + chr8_1:946127-946146 None:intergenic 35.0%
!! TACAGTAGAAGGTTCTGGAA+AGG - chr8_1:944365-944384 Msa1171330:three_prime_UTR 40.0%
!! GAAGGTTCTGGAAAGGAAAA+AGG - chr8_1:944372-944391 Msa1171330:three_prime_UTR 40.0%
GGATAGAATACTACGTAGCA+CGG + chr8_1:944994-945013 None:intergenic 40.0%
! GAACTGTTGCCCTTTTATGT+CGG - chr8_1:945360-945379 Msa1171330:intron 40.0%
ACCCTTCTTCCGACATAAAA+GGG + chr8_1:945372-945391 None:intergenic 40.0%
AACCCTTCTTCCGACATAAA+AGG + chr8_1:945373-945392 None:intergenic 40.0%
ACAATCAACTGCTTCTCATC+TGG + chr8_1:945450-945469 None:intergenic 40.0%
AAAACTTGTAAGAGGAGCAG+GGG + chr8_1:945484-945503 None:intergenic 40.0%
CAAAACTTGTAAGAGGAGCA+GGG + chr8_1:945485-945504 None:intergenic 40.0%
ACAAAACTTGTAAGAGGAGC+AGG + chr8_1:945486-945505 None:intergenic 40.0%
AGTCCATCAAAGCTGAGTTA+TGG - chr8_1:945535-945554 Msa1171330:intron 40.0%
TTGCAAGCTGACATAGCTTA+TGG + chr8_1:945767-945786 None:intergenic 40.0%
AAGCTATGTCAGCTTGCAAA+TGG - chr8_1:945768-945787 Msa1171330:intron 40.0%
AGGAGACATTACTGTTGTGT+TGG - chr8_1:945877-945896 Msa1171330:intron 40.0%
!! TACAGTAGAAGGTTCTGGAA+AGG - chr8_1:944365-944384 Msa1171330:three_prime_UTR 40.0%
!! GAAGGTTCTGGAAAGGAAAA+AGG - chr8_1:944372-944391 Msa1171330:three_prime_UTR 40.0%
GGATAGAATACTACGTAGCA+CGG + chr8_1:944994-945013 None:intergenic 40.0%
! GAACTGTTGCCCTTTTATGT+CGG - chr8_1:945360-945379 Msa1171330:intron 40.0%
ACCCTTCTTCCGACATAAAA+GGG + chr8_1:945372-945391 None:intergenic 40.0%
AACCCTTCTTCCGACATAAA+AGG + chr8_1:945373-945392 None:intergenic 40.0%
ACAATCAACTGCTTCTCATC+TGG + chr8_1:945450-945469 None:intergenic 40.0%
AAAACTTGTAAGAGGAGCAG+GGG + chr8_1:945484-945503 None:intergenic 40.0%
CAAAACTTGTAAGAGGAGCA+GGG + chr8_1:945485-945504 None:intergenic 40.0%
ACAAAACTTGTAAGAGGAGC+AGG + chr8_1:945486-945505 None:intergenic 40.0%
AGTCCATCAAAGCTGAGTTA+TGG - chr8_1:945535-945554 Msa1171330:intron 40.0%
TTGCAAGCTGACATAGCTTA+TGG + chr8_1:945767-945786 None:intergenic 40.0%
AAGCTATGTCAGCTTGCAAA+TGG - chr8_1:945768-945787 Msa1171330:intron 40.0%
AGGAGACATTACTGTTGTGT+TGG - chr8_1:945877-945896 Msa1171330:intron 40.0%
AACCTTCTACTGTACTCCTC+TGG + chr8_1:944359-944378 None:intergenic 45.0%
! AGGAGTACAGTAGAAGGTTC+TGG - chr8_1:944360-944379 Msa1171330:three_prime_UTR 45.0%
TGAAAAACGAAGCGGGAGAT+TGG + chr8_1:944435-944454 None:intergenic 45.0%
ACACAAAAGAACACGGAGCA+AGG + chr8_1:944584-944603 None:intergenic 45.0%
ATGTGACATTGTCAGTGCTG+CGG + chr8_1:944854-944873 None:intergenic 45.0%
TAACATGACACTGATGCACG+TGG + chr8_1:944921-944940 None:intergenic 45.0%
CTTCAAATCGAAGGCTTGTC+CGG + chr8_1:944968-944987 None:intergenic 45.0%
GCACGGATACTTCAAATCGA+AGG + chr8_1:944977-944996 None:intergenic 45.0%
! GCAACAGTTCTGCATTGACA+AGG + chr8_1:945350-945369 None:intergenic 45.0%
! TGCCCTTTTATGTCGGAAGA+AGG - chr8_1:945367-945386 Msa1171330:intron 45.0%
! GCCCTTTTATGTCGGAAGAA+GGG - chr8_1:945368-945387 Msa1171330:intron 45.0%
AAACTTGTAAGAGGAGCAGG+GGG + chr8_1:945483-945502 None:intergenic 45.0%
! GGTCCATAACTCAGCTTTGA+TGG + chr8_1:945541-945560 None:intergenic 45.0%
GCTGAGTTATGGACCAACTT+TGG - chr8_1:945546-945565 Msa1171330:intron 45.0%
! CTTGCTGTAACTCAACTCAC+AGG + chr8_1:945812-945831 None:intergenic 45.0%
AACCTTCTACTGTACTCCTC+TGG + chr8_1:944359-944378 None:intergenic 45.0%
! AGGAGTACAGTAGAAGGTTC+TGG - chr8_1:944360-944379 Msa1171330:three_prime_UTR 45.0%
TGAAAAACGAAGCGGGAGAT+TGG + chr8_1:944435-944454 None:intergenic 45.0%
ACACAAAAGAACACGGAGCA+AGG + chr8_1:944584-944603 None:intergenic 45.0%
ATGTGACATTGTCAGTGCTG+CGG + chr8_1:944854-944873 None:intergenic 45.0%
TAACATGACACTGATGCACG+TGG + chr8_1:944921-944940 None:intergenic 45.0%
CTTCAAATCGAAGGCTTGTC+CGG + chr8_1:944968-944987 None:intergenic 45.0%
GCACGGATACTTCAAATCGA+AGG + chr8_1:944977-944996 None:intergenic 45.0%
! GCAACAGTTCTGCATTGACA+AGG + chr8_1:945350-945369 None:intergenic 45.0%
! TGCCCTTTTATGTCGGAAGA+AGG - chr8_1:945367-945386 Msa1171330:intron 45.0%
! GCCCTTTTATGTCGGAAGAA+GGG - chr8_1:945368-945387 Msa1171330:intron 45.0%
AAACTTGTAAGAGGAGCAGG+GGG + chr8_1:945483-945502 None:intergenic 45.0%
! GGTCCATAACTCAGCTTTGA+TGG + chr8_1:945541-945560 None:intergenic 45.0%
GCTGAGTTATGGACCAACTT+TGG - chr8_1:945546-945565 Msa1171330:intron 45.0%
! CTTGCTGTAACTCAACTCAC+AGG + chr8_1:945812-945831 None:intergenic 45.0%
! GACCAGAGGAGTACAGTAGA+AGG - chr8_1:944354-944373 Msa1171330:three_prime_UTR 50.0%
AACACGGAGCAAGGAGAAAC+TGG + chr8_1:944575-944594 None:intergenic 50.0%
TGTGACATTGTCAGTGCTGC+GGG + chr8_1:944853-944872 None:intergenic 50.0%
AGATACTATCAGACACCGGC+CGG - chr8_1:944946-944965 Msa1171330:intron 50.0%
ACAAGGACTGCAGGGCTTAA+TGG + chr8_1:945333-945352 None:intergenic 50.0%
! CTGCATTGACAAGGACTGCA+GGG + chr8_1:945341-945360 None:intergenic 50.0%
! TCTGCATTGACAAGGACTGC+AGG + chr8_1:945342-945361 None:intergenic 50.0%
AAGGGTTCGTACAGTGATGC+AGG - chr8_1:945386-945405 Msa1171330:intron 50.0%
TCTGACGCTCTAGCTGTCAT+TGG - chr8_1:945417-945436 Msa1171330:intron 50.0%
! GACCAGAGGAGTACAGTAGA+AGG - chr8_1:944354-944373 Msa1171330:three_prime_UTR 50.0%
AACACGGAGCAAGGAGAAAC+TGG + chr8_1:944575-944594 None:intergenic 50.0%
TGTGACATTGTCAGTGCTGC+GGG + chr8_1:944853-944872 None:intergenic 50.0%
AGATACTATCAGACACCGGC+CGG - chr8_1:944946-944965 Msa1171330:intron 50.0%
ACAAGGACTGCAGGGCTTAA+TGG + chr8_1:945333-945352 None:intergenic 50.0%
! CTGCATTGACAAGGACTGCA+GGG + chr8_1:945341-945360 None:intergenic 50.0%
! TCTGCATTGACAAGGACTGC+AGG + chr8_1:945342-945361 None:intergenic 50.0%
AAGGGTTCGTACAGTGATGC+AGG - chr8_1:945386-945405 Msa1171330:intron 50.0%
TCTGACGCTCTAGCTGTCAT+TGG - chr8_1:945417-945436 Msa1171330:intron 50.0%
GAGACGAGAGAGTAGAGCGA+GGG + chr8_1:944404-944423 None:intergenic 55.0%
AAATCGAAGGCTTGTCCGGC+CGG + chr8_1:944964-944983 None:intergenic 55.0%
GAGACGAGAGAGTAGAGCGA+GGG + chr8_1:944404-944423 None:intergenic 55.0%
AAATCGAAGGCTTGTCCGGC+CGG + chr8_1:944964-944983 None:intergenic 55.0%
CGAGACGAGAGAGTAGAGCG+AGG + chr8_1:944405-944424 None:intergenic 60.0%
CGAGACGAGAGAGTAGAGCG+AGG + chr8_1:944405-944424 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 944349 946207 944349 ID=Msa1171330;Name=Msa1171330
chr8_1 mRNA 944349 946207 944349 ID=Msa1171330-mRNA-1;Parent=Msa1171330;Name=Msa1171330-mRNA-1;_AED=0.03;_eAED=0.03;_QI=140|1|1|1|0.5|0.33|3|392|106
chr8_1 exon 944349 944805 944349 ID=Msa1171330-mRNA-1:exon:270;Parent=Msa1171330-mRNA-1
chr8_1 exon 944977 945235 944977 ID=Msa1171330-mRNA-1:exon:269;Parent=Msa1171330-mRNA-1
chr8_1 exon 946071 946207 946071 ID=Msa1171330-mRNA-1:exon:268;Parent=Msa1171330-mRNA-1
chr8_1 five_prime_UTR 946071 946207 946071 ID=Msa1171330-mRNA-1:five_prime_utr;Parent=Msa1171330-mRNA-1
chr8_1 five_prime_UTR 945233 945235 945233 ID=Msa1171330-mRNA-1:five_prime_utr;Parent=Msa1171330-mRNA-1
chr8_1 CDS 944977 945232 944977 ID=Msa1171330-mRNA-1:cds;Parent=Msa1171330-mRNA-1
chr8_1 CDS 944741 944805 944741 ID=Msa1171330-mRNA-1:cds;Parent=Msa1171330-mRNA-1
chr8_1 three_prime_UTR 944349 944740 944349 ID=Msa1171330-mRNA-1:three_prime_utr;Parent=Msa1171330-mRNA-1
Gene Sequence

>Msa1171330

ATGGATCCATTAAGCCCTGCAGTCCTTGTCAATGCAGAACTGTTGCCCTTTTATGTCGGAAGAAGGGTTCGTACAGTGATGCAGGTTGTGCGATCTGACGCTCTAGCTGTCATTGGAAAATCACCAGATGAGAAGCAGTTGATTGTAAAAGGATCACCCCCTGCTCCTCTTACAAGTTTTGTAGAAGTTATTGGTATTGTTAACAGTGACAAGTCCATCAAAGCTGAGTTATGGACCAACTTTGGTGATGAAATTGATATGCTCAGCTACCATAAGCTATGTCAGCTTGCAAATGGAGAATTTAAACACTTGTTCCTGTGA
Protein sequence

>Msa1171330

MDPLSPAVLVNAELLPFYVGRRVRTVMQVVRSDALAVIGKSPDEKQLIVKGSPPAPLTSFVEVIGIVNSDKSIKAELWTNFGDEIDMLSYHKLCQLANGEFKHLFL*