AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0790820

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0790820 MtrunA17_Chr7g0266481 98.113 106 2 0 1 106 1 106 1.35e-74 216
Msa0790820 MtrunA17_Chr1g0210621 54.206 107 48 1 1 106 1 107 2.05e-38 124
Msa0790820 MtrunA17_Chr1g0210651 55.140 107 47 1 1 106 1 107 7.15e-38 123
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0790820 AT4G18590.1 58.491 106 44 0 1 106 1 106 2.75e-46 144
Msa0790820 AT3G52630.2 55.140 107 47 1 1 106 1 107 7.74e-42 133
Msa0790820 AT3G52630.1 55.140 107 47 1 1 106 1 107 7.74e-42 133

Find 42 sgRNAs with CRISPR-Local

Find 197 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACCCTTCTTCCGACATAAA+AGG 0.241109 5_3:+1648122 None:intergenic
TCTGTGAACTATCATCTAAA+AGG 0.263776 5_3:+1647317 None:intergenic
ACAAGGACTGCAGGGCTTAA+TGG 0.275580 5_3:+1648162 None:intergenic
GAGAAGCAGTTGATTGTAAA+AGG 0.288767 5_3:-1648039 Msa0790820:CDS
ATGCTGATCACTGCATTAAA+AGG 0.296057 5_3:+1647368 None:intergenic
ACCAACTTTGGTGATGAAAT+TGG 0.344480 5_3:-1647934 Msa0790820:intron
TTGCAAGCTGACATAGCTTA+TGG 0.355631 5_3:+1647728 None:intergenic
GGTCCATAACTCAGCTTTGA+TGG 0.369681 5_3:+1647954 None:intergenic
AGGAGTACAGTAGAAGGTTC+TGG 0.374761 5_3:-1649132 Msa0790820:five_prime_UTR
ACAATCAACTGCTTCTCATC+TGG 0.376539 5_3:+1648045 None:intergenic
CTGTGAACTATCATCTAAAA+GGG 0.380041 5_3:+1647318 None:intergenic
GAAGGTTCTGGAAAGGAAAA+AGG 0.382096 5_3:-1649120 Msa0790820:five_prime_UTR
ATCTGCAAATTGAATCAATC+TGG 0.421426 5_3:-1649028 Msa0790820:intron
ACCAATTTCATCACCAAAGT+TGG 0.430140 5_3:+1647933 None:intergenic
ACAAAGACATCTCTATCATA+TGG 0.434457 5_3:-1647647 Msa0790820:three_prime_UTR
ATGTTGAGAAATAGATTTGT+TGG 0.434718 5_3:-1647423 Msa0790820:three_prime_UTR
ACAAAACTTGTAAGAGGAGC+AGG 0.439485 5_3:+1648009 None:intergenic
ACTTCTACAAAACTTGTAAG+AGG 0.459678 5_3:+1648003 None:intergenic
GCTGAGTTATGGACCAACTT+TGG 0.463853 5_3:-1647946 Msa0790820:CDS
TCTGCATTGACAAGGACTGC+AGG 0.468536 5_3:+1648153 None:intergenic
ACCCTTCTTCCGACATAAAA+GGG 0.468915 5_3:+1648123 None:intergenic
AAGCTATGTCAGCTTGCAAA+TGG 0.472567 5_3:-1647724 Msa0790820:CDS
TCTGACGCTCTAGCTGTCAT+TGG 0.479697 5_3:-1648075 Msa0790820:CDS
GACCAGAGGAGTACAGTAGA+AGG 0.493178 5_3:-1649138 Msa0790820:five_prime_UTR
AGTCCATCAAAGCTGAGTTA+TGG 0.498331 5_3:-1647957 Msa0790820:CDS
AACCTTCTACTGTACTCCTC+TGG 0.508205 5_3:+1649136 None:intergenic
AAGGGTTCGTACAGTGATGC+AGG 0.527340 5_3:-1648106 Msa0790820:CDS
AGGAGACATTACTGTTGTGT+TGG 0.530242 5_3:-1647615 Msa0790820:three_prime_UTR
CAAGCAGATATTATTTACCA+TGG 0.547450 5_3:+1647563 None:intergenic
CTGCATTGACAAGGACTGCA+GGG 0.550141 5_3:+1648154 None:intergenic
AAGCAGATATTATTTACCAT+GGG 0.598106 5_3:+1647564 None:intergenic
ATAAATAGAGGGAGGACCAG+AGG 0.608531 5_3:-1649152 None:intergenic
CAAAACTTGTAAGAGGAGCA+GGG 0.615820 5_3:+1648010 None:intergenic
TACAGTAGAAGGTTCTGGAA+AGG 0.618140 5_3:-1649127 Msa0790820:five_prime_UTR
CTATCATATGGAATATCTAG+AGG 0.627927 5_3:-1647635 Msa0790820:three_prime_UTR
GAGACGAGAGAGTAGAGCGA+GGG 0.635654 5_3:+1649091 None:intergenic
AAAACTTGTAAGAGGAGCAG+GGG 0.643901 5_3:+1648011 None:intergenic
CTTGCTGTAACTCAACTCAC+AGG 0.644780 5_3:+1647683 None:intergenic
TGTGAACTATCATCTAAAAG+GGG 0.646431 5_3:+1647319 None:intergenic
GCAACAGTTCTGCATTGACA+AGG 0.657999 5_3:+1648145 None:intergenic
CGAGACGAGAGAGTAGAGCG+AGG 0.669211 5_3:+1649090 None:intergenic
AAACTTGTAAGAGGAGCAGG+GGG 0.714728 5_3:+1648012 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATTTAAACAATTTAAACA+AGG + chr5_3:1647732-1647751 None:intergenic 10.0%
!! ATTAAAATTTAAATGAAATG+TGG - chr5_3:1647748-1647767 Msa0790820:intron 10.0%
!!! TGAAATCTATAAATTTTTTT+CGG - chr5_3:1648919-1648938 Msa0790820:intron 10.0%
!! TAATTTAAACAATTTAAACA+AGG + chr5_3:1647732-1647751 None:intergenic 10.0%
!! ATTAAAATTTAAATGAAATG+TGG - chr5_3:1647748-1647767 Msa0790820:intron 10.0%
!!! TGAAATCTATAAATTTTTTT+CGG - chr5_3:1648919-1648938 Msa0790820:intron 10.0%
!!! GAAATCTATAAATTTTTTTC+GGG - chr5_3:1648920-1648939 Msa0790820:intron 15.0%
!!! AAATCTATAAATTTTTTTCG+GGG - chr5_3:1648921-1648940 Msa0790820:intron 15.0%
!!! GAAATCTATAAATTTTTTTC+GGG - chr5_3:1648920-1648939 Msa0790820:intron 15.0%
!!! AAATCTATAAATTTTTTTCG+GGG - chr5_3:1648921-1648940 Msa0790820:intron 15.0%
!!! CAATTTTCTCAAAATACTAT+CGG + chr5_3:1647840-1647859 None:intergenic 20.0%
!!! GTTAGTTCTTTTGTATATTT+TGG - chr5_3:1647987-1648006 Msa0790820:CDS 20.0%
!!! AGTGTTTTCCTATAATTTTT+AGG + chr5_3:1648037-1648056 None:intergenic 20.0%
!! GTAATGATATTTATTCTCTA+TGG - chr5_3:1648609-1648628 Msa0790820:intron 20.0%
!! TTGAAATTTCACTTATAACA+AGG + chr5_3:1648636-1648655 None:intergenic 20.0%
!!! CAATTTTCTCAAAATACTAT+CGG + chr5_3:1647840-1647859 None:intergenic 20.0%
!!! GTTAGTTCTTTTGTATATTT+TGG - chr5_3:1647987-1648006 Msa0790820:CDS 20.0%
!!! AGTGTTTTCCTATAATTTTT+AGG + chr5_3:1648037-1648056 None:intergenic 20.0%
!! GTAATGATATTTATTCTCTA+TGG - chr5_3:1648609-1648628 Msa0790820:intron 20.0%
!! TTGAAATTTCACTTATAACA+AGG + chr5_3:1648636-1648655 None:intergenic 20.0%
! CAATCATGAACACAAAATTA+GGG + chr5_3:1647584-1647603 None:intergenic 25.0%
! TCAATCATGAACACAAAATT+AGG + chr5_3:1647585-1647604 None:intergenic 25.0%
! TATTTGTAGATTTGACTGAA+GGG - chr5_3:1647670-1647689 Msa0790820:three_prime_UTR 25.0%
! ACTAACTAGATAGGTATAAA+TGG + chr5_3:1647973-1647992 None:intergenic 25.0%
! TACAAAAGAACTAACTAGAT+AGG + chr5_3:1647982-1648001 None:intergenic 25.0%
!!! TTTCCTATAATTTTTAGGAG+CGG + chr5_3:1648032-1648051 None:intergenic 25.0%
!! CTTAATTTGGAGTCAAAATA+AGG + chr5_3:1648234-1648253 None:intergenic 25.0%
!!! ACAAGTTTTGTAGAAGTTAT+TGG - chr5_3:1648453-1648472 Msa0790820:intron 25.0%
!!! ATATTTTGTTTTTGTTCCCA+TGG - chr5_3:1648870-1648889 Msa0790820:intron 25.0%
! AAGCAGATATTATTTACCAT+GGG + chr5_3:1648889-1648908 None:intergenic 25.0%
! ATGTTGAGAAATAGATTTGT+TGG - chr5_3:1649027-1649046 Msa0790820:intron 25.0%
!! ATAGTTCACAGATTGATTTA+TGG - chr5_3:1649144-1649163 Msa0790820:five_prime_UTR 25.0%
!! TAGTTCACAGATTGATTTAT+GGG - chr5_3:1649145-1649164 Msa0790820:five_prime_UTR 25.0%
! CAATCATGAACACAAAATTA+GGG + chr5_3:1647584-1647603 None:intergenic 25.0%
! TCAATCATGAACACAAAATT+AGG + chr5_3:1647585-1647604 None:intergenic 25.0%
! TATTTGTAGATTTGACTGAA+GGG - chr5_3:1647670-1647689 Msa0790820:three_prime_UTR 25.0%
! ACTAACTAGATAGGTATAAA+TGG + chr5_3:1647973-1647992 None:intergenic 25.0%
! TACAAAAGAACTAACTAGAT+AGG + chr5_3:1647982-1648001 None:intergenic 25.0%
!!! TTTCCTATAATTTTTAGGAG+CGG + chr5_3:1648032-1648051 None:intergenic 25.0%
!! CTTAATTTGGAGTCAAAATA+AGG + chr5_3:1648234-1648253 None:intergenic 25.0%
!!! ACAAGTTTTGTAGAAGTTAT+TGG - chr5_3:1648453-1648472 Msa0790820:intron 25.0%
!!! ATATTTTGTTTTTGTTCCCA+TGG - chr5_3:1648870-1648889 Msa0790820:intron 25.0%
! AAGCAGATATTATTTACCAT+GGG + chr5_3:1648889-1648908 None:intergenic 25.0%
! ATGTTGAGAAATAGATTTGT+TGG - chr5_3:1649027-1649046 Msa0790820:intron 25.0%
ATCTGCAAATTGAATCAATC+TGG - chr5_3:1647422-1647441 Msa0790820:three_prime_UTR 30.0%
AATGAAACGATGAATTGGAA+AGG + chr5_3:1647455-1647474 None:intergenic 30.0%
TAGAGAATGAAACGATGAAT+TGG + chr5_3:1647460-1647479 None:intergenic 30.0%
! GTGTAAAGAAGCATGAAAAA+TGG + chr5_3:1647488-1647507 None:intergenic 30.0%
ACAGAAAACACAAAAGAACA+CGG + chr5_3:1647549-1647568 None:intergenic 30.0%
GTATTTGTAGATTTGACTGA+AGG - chr5_3:1647669-1647688 Msa0790820:three_prime_UTR 30.0%
! AATTACAGTTTTACCGAATC+AGG - chr5_3:1648082-1648101 Msa0790820:CDS 30.0%
ATTTCAAATGTCTCCTGATT+CGG + chr5_3:1648098-1648117 None:intergenic 30.0%
GATGCATTATTACATTAAGC+TGG - chr5_3:1648142-1648161 Msa0790820:CDS 30.0%
!! TAAGGTGAAAAAGGTATTGA+TGG + chr5_3:1648216-1648235 None:intergenic 30.0%
GAGTCAAAATAAGGTGAAAA+AGG + chr5_3:1648225-1648244 None:intergenic 30.0%
CACAACAAGATGACTTAATT+TGG + chr5_3:1648247-1648266 None:intergenic 30.0%
! TGTGCATATTTTGCAGAATA+TGG - chr5_3:1648263-1648282 Msa0790820:intron 30.0%
ACTTCTACAAAACTTGTAAG+AGG + chr5_3:1648450-1648469 None:intergenic 30.0%
! TTTATTCCCTTTTCTCAAAC+TGG - chr5_3:1648547-1648566 Msa0790820:intron 30.0%
TTTCTAAGCATATTAAGCTG+TGG - chr5_3:1648657-1648676 Msa0790820:intron 30.0%
TTCTAAGCATATTAAGCTGT+GGG - chr5_3:1648658-1648677 Msa0790820:intron 30.0%
ACAAAGACATCTCTATCATA+TGG - chr5_3:1648803-1648822 Msa0790820:intron 30.0%
CTATCATATGGAATATCTAG+AGG - chr5_3:1648815-1648834 Msa0790820:intron 30.0%
CAAGCAGATATTATTTACCA+TGG + chr5_3:1648890-1648909 None:intergenic 30.0%
TGTGAACTATCATCTAAAAG+GGG + chr5_3:1649134-1649153 None:intergenic 30.0%
CTGTGAACTATCATCTAAAA+GGG + chr5_3:1649135-1649154 None:intergenic 30.0%
TCTGTGAACTATCATCTAAA+AGG + chr5_3:1649136-1649155 None:intergenic 30.0%
! AGTTCACAGATTGATTTATG+GGG - chr5_3:1649146-1649165 Msa0790820:five_prime_UTR 30.0%
ATCTGCAAATTGAATCAATC+TGG - chr5_3:1647422-1647441 Msa0790820:three_prime_UTR 30.0%
AATGAAACGATGAATTGGAA+AGG + chr5_3:1647455-1647474 None:intergenic 30.0%
TAGAGAATGAAACGATGAAT+TGG + chr5_3:1647460-1647479 None:intergenic 30.0%
! GTGTAAAGAAGCATGAAAAA+TGG + chr5_3:1647488-1647507 None:intergenic 30.0%
ACAGAAAACACAAAAGAACA+CGG + chr5_3:1647549-1647568 None:intergenic 30.0%
GTATTTGTAGATTTGACTGA+AGG - chr5_3:1647669-1647688 Msa0790820:three_prime_UTR 30.0%
! AATTACAGTTTTACCGAATC+AGG - chr5_3:1648082-1648101 Msa0790820:CDS 30.0%
ATTTCAAATGTCTCCTGATT+CGG + chr5_3:1648098-1648117 None:intergenic 30.0%
GATGCATTATTACATTAAGC+TGG - chr5_3:1648142-1648161 Msa0790820:CDS 30.0%
!! TAAGGTGAAAAAGGTATTGA+TGG + chr5_3:1648216-1648235 None:intergenic 30.0%
GAGTCAAAATAAGGTGAAAA+AGG + chr5_3:1648225-1648244 None:intergenic 30.0%
CACAACAAGATGACTTAATT+TGG + chr5_3:1648247-1648266 None:intergenic 30.0%
! TGTGCATATTTTGCAGAATA+TGG - chr5_3:1648263-1648282 Msa0790820:intron 30.0%
ACTTCTACAAAACTTGTAAG+AGG + chr5_3:1648450-1648469 None:intergenic 30.0%
! TTTATTCCCTTTTCTCAAAC+TGG - chr5_3:1648547-1648566 Msa0790820:intron 30.0%
TTTCTAAGCATATTAAGCTG+TGG - chr5_3:1648657-1648676 Msa0790820:intron 30.0%
TTCTAAGCATATTAAGCTGT+GGG - chr5_3:1648658-1648677 Msa0790820:intron 30.0%
ACAAAGACATCTCTATCATA+TGG - chr5_3:1648803-1648822 Msa0790820:intron 30.0%
CTATCATATGGAATATCTAG+AGG - chr5_3:1648815-1648834 Msa0790820:intron 30.0%
CAAGCAGATATTATTTACCA+TGG + chr5_3:1648890-1648909 None:intergenic 30.0%
TGTGAACTATCATCTAAAAG+GGG + chr5_3:1649134-1649153 None:intergenic 30.0%
CTGTGAACTATCATCTAAAA+GGG + chr5_3:1649135-1649154 None:intergenic 30.0%
TCTGTGAACTATCATCTAAA+AGG + chr5_3:1649136-1649155 None:intergenic 30.0%
AAATTGCTGAAAAACGAAGC+GGG + chr5_3:1647400-1647419 None:intergenic 35.0%
CAAATTGCTGAAAAACGAAG+CGG + chr5_3:1647401-1647420 None:intergenic 35.0%
TTCAAGATACTATCAGACAC+CGG - chr5_3:1647900-1647919 Msa0790820:intron 35.0%
!! CTATAATTTTTAGGAGCGGA+AGG + chr5_3:1648028-1648047 None:intergenic 35.0%
CTTCCGCTCCTAAAAATTAT+AGG - chr5_3:1648026-1648045 Msa0790820:CDS 35.0%
! GAGACATTTGAAATCTGTAC+AGG - chr5_3:1648104-1648123 Msa0790820:CDS 35.0%
GAGAAGCAGTTGATTGTAAA+AGG - chr5_3:1648411-1648430 Msa0790820:intron 35.0%
!! ACCAACTTTGGTGATGAAAT+TGG - chr5_3:1648516-1648535 Msa0790820:intron 35.0%
ACCAATTTCATCACCAAAGT+TGG + chr5_3:1648520-1648539 None:intergenic 35.0%
TGACAACCAGTTTGAGAAAA+GGG + chr5_3:1648556-1648575 None:intergenic 35.0%
ATGACAACCAGTTTGAGAAA+AGG + chr5_3:1648557-1648576 None:intergenic 35.0%
ATGCTGATCACTGCATTAAA+AGG + chr5_3:1649085-1649104 None:intergenic 35.0%
AAATTGCTGAAAAACGAAGC+GGG + chr5_3:1647400-1647419 None:intergenic 35.0%
CAAATTGCTGAAAAACGAAG+CGG + chr5_3:1647401-1647420 None:intergenic 35.0%
TTCAAGATACTATCAGACAC+CGG - chr5_3:1647900-1647919 Msa0790820:intron 35.0%
!! CTATAATTTTTAGGAGCGGA+AGG + chr5_3:1648028-1648047 None:intergenic 35.0%
CTTCCGCTCCTAAAAATTAT+AGG - chr5_3:1648026-1648045 Msa0790820:CDS 35.0%
! GAGACATTTGAAATCTGTAC+AGG - chr5_3:1648104-1648123 Msa0790820:CDS 35.0%
GAGAAGCAGTTGATTGTAAA+AGG - chr5_3:1648411-1648430 Msa0790820:intron 35.0%
!! ACCAACTTTGGTGATGAAAT+TGG - chr5_3:1648516-1648535 Msa0790820:intron 35.0%
ACCAATTTCATCACCAAAGT+TGG + chr5_3:1648520-1648539 None:intergenic 35.0%
TGACAACCAGTTTGAGAAAA+GGG + chr5_3:1648556-1648575 None:intergenic 35.0%
ATGACAACCAGTTTGAGAAA+AGG + chr5_3:1648557-1648576 None:intergenic 35.0%
ATGCTGATCACTGCATTAAA+AGG + chr5_3:1649085-1649104 None:intergenic 35.0%
!! TACAGTAGAAGGTTCTGGAA+AGG - chr5_3:1647323-1647342 Msa0790820:three_prime_UTR 40.0%
!! GAAGGTTCTGGAAAGGAAAA+AGG - chr5_3:1647330-1647349 Msa0790820:three_prime_UTR 40.0%
GGATAGAATACTACGTAGCA+CGG + chr5_3:1647952-1647971 None:intergenic 40.0%
! GAACTGTTGCCCTTTTATGT+CGG - chr5_3:1648318-1648337 Msa0790820:intron 40.0%
ACCCTTCTTCCGACATAAAA+GGG + chr5_3:1648330-1648349 None:intergenic 40.0%
AACCCTTCTTCCGACATAAA+AGG + chr5_3:1648331-1648350 None:intergenic 40.0%
ACAATCAACTGCTTCTCATC+TGG + chr5_3:1648408-1648427 None:intergenic 40.0%
AAAACTTGTAAGAGGAGCAG+GGG + chr5_3:1648442-1648461 None:intergenic 40.0%
CAAAACTTGTAAGAGGAGCA+GGG + chr5_3:1648443-1648462 None:intergenic 40.0%
ACAAAACTTGTAAGAGGAGC+AGG + chr5_3:1648444-1648463 None:intergenic 40.0%
AGTCCATCAAAGCTGAGTTA+TGG - chr5_3:1648493-1648512 Msa0790820:intron 40.0%
TTGCAAGCTGACATAGCTTA+TGG + chr5_3:1648725-1648744 None:intergenic 40.0%
AAGCTATGTCAGCTTGCAAA+TGG - chr5_3:1648726-1648745 Msa0790820:intron 40.0%
AGGAGACATTACTGTTGTGT+TGG - chr5_3:1648835-1648854 Msa0790820:intron 40.0%
!! TACAGTAGAAGGTTCTGGAA+AGG - chr5_3:1647323-1647342 Msa0790820:three_prime_UTR 40.0%
!! GAAGGTTCTGGAAAGGAAAA+AGG - chr5_3:1647330-1647349 Msa0790820:three_prime_UTR 40.0%
GGATAGAATACTACGTAGCA+CGG + chr5_3:1647952-1647971 None:intergenic 40.0%
! GAACTGTTGCCCTTTTATGT+CGG - chr5_3:1648318-1648337 Msa0790820:intron 40.0%
ACCCTTCTTCCGACATAAAA+GGG + chr5_3:1648330-1648349 None:intergenic 40.0%
AACCCTTCTTCCGACATAAA+AGG + chr5_3:1648331-1648350 None:intergenic 40.0%
ACAATCAACTGCTTCTCATC+TGG + chr5_3:1648408-1648427 None:intergenic 40.0%
AAAACTTGTAAGAGGAGCAG+GGG + chr5_3:1648442-1648461 None:intergenic 40.0%
CAAAACTTGTAAGAGGAGCA+GGG + chr5_3:1648443-1648462 None:intergenic 40.0%
ACAAAACTTGTAAGAGGAGC+AGG + chr5_3:1648444-1648463 None:intergenic 40.0%
AGTCCATCAAAGCTGAGTTA+TGG - chr5_3:1648493-1648512 Msa0790820:intron 40.0%
TTGCAAGCTGACATAGCTTA+TGG + chr5_3:1648725-1648744 None:intergenic 40.0%
AAGCTATGTCAGCTTGCAAA+TGG - chr5_3:1648726-1648745 Msa0790820:intron 40.0%
AGGAGACATTACTGTTGTGT+TGG - chr5_3:1648835-1648854 Msa0790820:intron 40.0%
AACCTTCTACTGTACTCCTC+TGG + chr5_3:1647317-1647336 None:intergenic 45.0%
! AGGAGTACAGTAGAAGGTTC+TGG - chr5_3:1647318-1647337 Msa0790820:three_prime_UTR 45.0%
TGAAAAACGAAGCGGGAGAT+TGG + chr5_3:1647393-1647412 None:intergenic 45.0%
ACACAAAAGAACACGGAGCA+AGG + chr5_3:1647542-1647561 None:intergenic 45.0%
ATGTGACATTGTCAGTGCTG+CGG + chr5_3:1647812-1647831 None:intergenic 45.0%
TAACATGACACTGATGCACG+TGG + chr5_3:1647879-1647898 None:intergenic 45.0%
CTTCAAATCGAAGGCTTGTC+CGG + chr5_3:1647926-1647945 None:intergenic 45.0%
GCACGGATACTTCAAATCGA+AGG + chr5_3:1647935-1647954 None:intergenic 45.0%
! GCAACAGTTCTGCATTGACA+AGG + chr5_3:1648308-1648327 None:intergenic 45.0%
! TGCCCTTTTATGTCGGAAGA+AGG - chr5_3:1648325-1648344 Msa0790820:intron 45.0%
! GCCCTTTTATGTCGGAAGAA+GGG - chr5_3:1648326-1648345 Msa0790820:intron 45.0%
AAACTTGTAAGAGGAGCAGG+GGG + chr5_3:1648441-1648460 None:intergenic 45.0%
! GGTCCATAACTCAGCTTTGA+TGG + chr5_3:1648499-1648518 None:intergenic 45.0%
GCTGAGTTATGGACCAACTT+TGG - chr5_3:1648504-1648523 Msa0790820:intron 45.0%
! CTTGCTGTAACTCAACTCAC+AGG + chr5_3:1648770-1648789 None:intergenic 45.0%
AACCTTCTACTGTACTCCTC+TGG + chr5_3:1647317-1647336 None:intergenic 45.0%
! AGGAGTACAGTAGAAGGTTC+TGG - chr5_3:1647318-1647337 Msa0790820:three_prime_UTR 45.0%
TGAAAAACGAAGCGGGAGAT+TGG + chr5_3:1647393-1647412 None:intergenic 45.0%
ACACAAAAGAACACGGAGCA+AGG + chr5_3:1647542-1647561 None:intergenic 45.0%
ATGTGACATTGTCAGTGCTG+CGG + chr5_3:1647812-1647831 None:intergenic 45.0%
TAACATGACACTGATGCACG+TGG + chr5_3:1647879-1647898 None:intergenic 45.0%
CTTCAAATCGAAGGCTTGTC+CGG + chr5_3:1647926-1647945 None:intergenic 45.0%
GCACGGATACTTCAAATCGA+AGG + chr5_3:1647935-1647954 None:intergenic 45.0%
! GCAACAGTTCTGCATTGACA+AGG + chr5_3:1648308-1648327 None:intergenic 45.0%
! TGCCCTTTTATGTCGGAAGA+AGG - chr5_3:1648325-1648344 Msa0790820:intron 45.0%
! GCCCTTTTATGTCGGAAGAA+GGG - chr5_3:1648326-1648345 Msa0790820:intron 45.0%
AAACTTGTAAGAGGAGCAGG+GGG + chr5_3:1648441-1648460 None:intergenic 45.0%
! GGTCCATAACTCAGCTTTGA+TGG + chr5_3:1648499-1648518 None:intergenic 45.0%
GCTGAGTTATGGACCAACTT+TGG - chr5_3:1648504-1648523 Msa0790820:intron 45.0%
! CTTGCTGTAACTCAACTCAC+AGG + chr5_3:1648770-1648789 None:intergenic 45.0%
! GACCAGAGGAGTACAGTAGA+AGG - chr5_3:1647312-1647331 Msa0790820:three_prime_UTR 50.0%
AACACGGAGCAAGGAGAAAC+TGG + chr5_3:1647533-1647552 None:intergenic 50.0%
TGTGACATTGTCAGTGCTGC+GGG + chr5_3:1647811-1647830 None:intergenic 50.0%
AGATACTATCAGACACCGGC+CGG - chr5_3:1647904-1647923 Msa0790820:intron 50.0%
ACAAGGACTGCAGGGCTTAA+TGG + chr5_3:1648291-1648310 None:intergenic 50.0%
! CTGCATTGACAAGGACTGCA+GGG + chr5_3:1648299-1648318 None:intergenic 50.0%
! TCTGCATTGACAAGGACTGC+AGG + chr5_3:1648300-1648319 None:intergenic 50.0%
AAGGGTTCGTACAGTGATGC+AGG - chr5_3:1648344-1648363 Msa0790820:intron 50.0%
TCTGACGCTCTAGCTGTCAT+TGG - chr5_3:1648375-1648394 Msa0790820:intron 50.0%
! GACCAGAGGAGTACAGTAGA+AGG - chr5_3:1647312-1647331 Msa0790820:three_prime_UTR 50.0%
AACACGGAGCAAGGAGAAAC+TGG + chr5_3:1647533-1647552 None:intergenic 50.0%
TGTGACATTGTCAGTGCTGC+GGG + chr5_3:1647811-1647830 None:intergenic 50.0%
AGATACTATCAGACACCGGC+CGG - chr5_3:1647904-1647923 Msa0790820:intron 50.0%
ACAAGGACTGCAGGGCTTAA+TGG + chr5_3:1648291-1648310 None:intergenic 50.0%
! CTGCATTGACAAGGACTGCA+GGG + chr5_3:1648299-1648318 None:intergenic 50.0%
! TCTGCATTGACAAGGACTGC+AGG + chr5_3:1648300-1648319 None:intergenic 50.0%
AAGGGTTCGTACAGTGATGC+AGG - chr5_3:1648344-1648363 Msa0790820:intron 50.0%
TCTGACGCTCTAGCTGTCAT+TGG - chr5_3:1648375-1648394 Msa0790820:intron 50.0%
GAGACGAGAGAGTAGAGCGA+GGG + chr5_3:1647362-1647381 None:intergenic 55.0%
AAATCGAAGGCTTGTCCGGC+CGG + chr5_3:1647922-1647941 None:intergenic 55.0%
GAGACGAGAGAGTAGAGCGA+GGG + chr5_3:1647362-1647381 None:intergenic 55.0%
AAATCGAAGGCTTGTCCGGC+CGG + chr5_3:1647922-1647941 None:intergenic 55.0%
CGAGACGAGAGAGTAGAGCG+AGG + chr5_3:1647363-1647382 None:intergenic 60.0%
CGAGACGAGAGAGTAGAGCG+AGG + chr5_3:1647363-1647382 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr5_3 gene 1647307 1649165 1647307 ID=Msa0790820;Name=Msa0790820
chr5_3 mRNA 1647307 1649165 1647307 ID=Msa0790820-mRNA-1;Parent=Msa0790820;Name=Msa0790820-mRNA-1;_AED=0.03;_eAED=0.03;_QI=140|1|1|1|0.5|0.33|3|392|106
chr5_3 exon 1647307 1647763 1647307 ID=Msa0790820-mRNA-1:exon:615;Parent=Msa0790820-mRNA-1
chr5_3 exon 1647935 1648193 1647935 ID=Msa0790820-mRNA-1:exon:614;Parent=Msa0790820-mRNA-1
chr5_3 exon 1649029 1649165 1649029 ID=Msa0790820-mRNA-1:exon:613;Parent=Msa0790820-mRNA-1
chr5_3 five_prime_UTR 1649029 1649165 1649029 ID=Msa0790820-mRNA-1:five_prime_utr;Parent=Msa0790820-mRNA-1
chr5_3 five_prime_UTR 1648191 1648193 1648191 ID=Msa0790820-mRNA-1:five_prime_utr;Parent=Msa0790820-mRNA-1
chr5_3 CDS 1647935 1648190 1647935 ID=Msa0790820-mRNA-1:cds;Parent=Msa0790820-mRNA-1
chr5_3 CDS 1647699 1647763 1647699 ID=Msa0790820-mRNA-1:cds;Parent=Msa0790820-mRNA-1
chr5_3 three_prime_UTR 1647307 1647698 1647307 ID=Msa0790820-mRNA-1:three_prime_utr;Parent=Msa0790820-mRNA-1
Gene Sequence

>Msa0790820

ATGGATCCATTAAGCCCTGCAGTCCTTGTCAATGCAGAACTGTTGCCCTTTTATGTCGGAAGAAGGGTTCGTACAGTGATGCAGGTTGTGCGATCTGACGCTCTAGCTGTCATTGGAAAATCACCAGATGAGAAGCAGTTGATTGTAAAAGGATCACCCCCTGCTCCTCTTACAAGTTTTGTAGAAGTTATTGGTATTGTTAACAGTGACAAGTCCATCAAAGCTGAGTTATGGACCAACTTTGGTGATGAAATTGATATGCTCAGCTACCATAAGCTATGTCAGCTTGCAAATGGAGAATTTAAACACTTGTTCCTGTGA
Protein sequence

>Msa0790820

MDPLSPAVLVNAELLPFYVGRRVRTVMQVVRSDALAVIGKSPDEKQLIVKGSPPAPLTSFVEVIGIVNSDKSIKAELWTNFGDEIDMLSYHKLCQLANGEFKHLFL*