AlfalfaGEDB | Msa1172140

M. sativa cultivar zhongmu-4 / Msa1172140

KEGG: -
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1172140	XP_003608943.1	95.395	152	4	1	1	152	1	149	7.47e-102	298

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0043670	Msa1172140	0.810594	1.066576e-50	-8.615850e-47
Msa0092740	Msa1172140	0.803929	2.756930e-49	-8.615850e-47
Msa0137610	Msa1172140	0.812968	3.245666e-51	-8.615850e-47
Msa1172040	Msa1172140	0.983446	9.167874e-158	-8.615850e-47
Msa1172100	Msa1172140	0.982365	6.629113e-155	-8.615850e-47
Msa1172140	Msa1440260	0.807802	4.230887e-50	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1172140	MtrunA17_Chr4g0059251	95.395	152	4	1	1	152	1	149	6.87e-106	298
Msa1172140	MtrunA17_Chr3g0117901	50.407	123	32	5	22	135	20	122	3.59e-31	109
Msa1172140	MtrunA17_Chr6g0451141	42.857	126	57	6	17	137	25	140	6.72e-24	90.9
Msa1172140	MtrunA17_Chr2g0305131	46.835	79	35	4	58	134	57	130	3.28e-13	62.8
Msa1172140	MtrunA17_Chr4g0065221	28.889	135	88	4	1	133	9	137	3.84e-12	60.5

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1172140	AT5G62865.1	41.447	152	66	7	3	139	1	144	9.02e-19	78.6
Msa1172140	AT3G01430.1	34.194	155	59	6	14	137	21	163	1.08e-17	75.9
Msa1172140	AT3G48020.1	37.705	122	55	4	26	139	14	122	1.99e-16	71.6
Msa1172140	AT5G14895.1	35.252	139	60	7	17	137	24	150	3.40e-16	71.6

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Find 56 sgRNAs with CRISPR-Local

Find 61 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGAAACTCCGAGCCTGGT+CGG	0.644913	8_1:+2008173	Msa1172140:CDS
AACAGACTTAATATTAACGG+CGG	0.721385	8_1:-2008367	None:intergenic
AACCCGCCAATTTCACCCTC+CGG	0.509850	8_1:-2008399	None:intergenic
AACCGGAGGGTGAAATTGGC+GGG	0.518811	8_1:+2008397	Msa1172140:CDS
AAGTTTAACGCATAACTCAA+CGG	0.651799	8_1:-2008282	None:intergenic
AATGAAGATAGCCACGACGA+CGG	0.629695	8_1:+2008318	Msa1172140:CDS
AATTCCGAAACCCGTCGTCG+TGG	0.629946	8_1:-2008329	None:intergenic
ACCAGCGACGATCTCCGACC+AGG	0.547492	8_1:-2008187	None:intergenic
ACGCCGCCGTTCCGTTTGGT+GGG	0.538378	8_1:+2008059	Msa1172140:CDS
ACGGCGGCGTGAGGCGAAGC+AGG	0.472114	8_1:-2008046	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
CAAGATGGAAGAATTTCATT+CGG	+	chr8_1:2008211-2008230	Msa1172140:CDS	30.0%
AAGTTTAACGCATAACTCAA+CGG	-	chr8_1:2008285-2008304	None:intergenic	30.0%
TTATGCGTTAAACTTTGATG+AGG	+	chr8_1:2008290-2008309	Msa1172140:CDS	30.0%
TATGCGTTAAACTTTGATGA+GGG	+	chr8_1:2008291-2008310	Msa1172140:CDS	30.0%
AACAGACTTAATATTAACGG+CGG	-	chr8_1:2008370-2008389	None:intergenic	30.0%
CGCAACAGACTTAATATTAA+CGG	-	chr8_1:2008373-2008392	None:intergenic	30.0%
CGAATGAAATTCTTCCATCT+TGG	-	chr8_1:2008213-2008232	None:intergenic	35.0%
TAATATTAAGTCTGTTGCGC+CGG	+	chr8_1:2008374-2008393	Msa1172140:CDS	35.0%
ATGGGATTGAGAAAAGGAGG+CGG	-	chr8_1:2008097-2008116	None:intergenic	45.0%
TTGTCATACGCTTCGATCGA+TGG	-	chr8_1:2008248-2008267	None:intergenic	45.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_1	gene	2007970	2008428	2007970	ID=Msa1172140;Name=Msa1172140
chr8_1	mRNA	2007970	2008428	2007970	ID=Msa1172140-mRNA-1;Parent=Msa1172140;Name=Msa1172140-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|152
chr8_1	exon	2007970	2008428	2007970	ID=Msa1172140-mRNA-1:exon:753;Parent=Msa1172140-mRNA-1
chr8_1	CDS	2007970	2008428	2007970	ID=Msa1172140-mRNA-1:cds;Parent=Msa1172140-mRNA-1

Gene Sequence

>Msa1172140

ATGTCCGTTGAGCTAGAGCTTGAATCACCAACCGCTCATCTCCAACAAAAGAGATCAAGCGCCTTATGTTTCCCCACCTGCTTCGCCTCACGCCGCCGTTCCGTTTGGTGGGAACGTGTTCGCTCCGCCTCCTTTTCTCAATCCCATCCCACCACCACCACAACCACCGCCGATAGATGGTGGTCTCGCGGCCTCAACGCCTTAAAGAAACTCCGAGCCTGGTCGGAGATCGTCGCTGGTCCAAGATGGAAGAATTTCATTCGGAAATTCAACAACCATCGATCGAAGCGTATGACAAAGTGTCAATACGATCCGTTGAGTTATGCGTTAAACTTTGATGAGGGACAGAATGAAGATAGCCACGACGACGGGTTTCGGAATTTCTCTACGCGTTACGCCGCCGTTAATATTAAGTCTGTTGCGCCGGAACCGGAGGGTGAAATTGGCGGGTTAGTTTGA

Protein sequence

>Msa1172140

MSVELELESPTAHLQQKRSSALCFPTCFASRRRSVWWERVRSASFSQSHPTTTTTTADRWWSRGLNALKKLRAWSEIVAGPRWKNFIRKFNNHRSKRMTKCQYDPLSYALNFDEGQNEDSHDDGFRNFSTRYAAVNIKSVAPEPEGEIGGLV*