Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0002490 | Msa0043670 | -0.803809 | 2.919542e-49 | -8.615850e-47 |
| Msa0016670 | Msa0043670 | -0.832560 | 8.816577e-56 | -8.615850e-47 |
| Msa0027260 | Msa0043670 | -0.801726 | 7.858446e-49 | -8.615850e-47 |
| Msa0032370 | Msa0043670 | -0.810720 | 1.001729e-50 | -8.615850e-47 |
| Msa0032840 | Msa0043670 | 0.801215 | 1.000271e-48 | -8.615850e-47 |
| Msa0032920 | Msa0043670 | 0.853647 | 2.081113e-61 | -8.615850e-47 |
| Msa0032960 | Msa0043670 | 0.828731 | 7.635798e-55 | -8.615850e-47 |
| Msa0043670 | Msa0073670 | -0.823698 | 1.203737e-53 | -8.615850e-47 |
| Msa0043670 | Msa0077200 | -0.836618 | 8.419363e-57 | -8.615850e-47 |
| Msa0043670 | Msa0081520 | -0.806437 | 8.228436e-50 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0043670 | MtrunA17_Chr1g0209811 | 95.092 | 163 | 5 | 1 | 1 | 163 | 1 | 160 | 3.03e-106 | 300 |
| Msa0043670 | MtrunA17_Chr6g0457561 | 46.584 | 161 | 66 | 6 | 4 | 163 | 27 | 168 | 8.29e-35 | 119 |
| Msa0043670 | MtrunA17_Chr8g0391011 | 46.012 | 163 | 67 | 8 | 7 | 163 | 13 | 160 | 5.04e-29 | 104 |
| Msa0043670 | MtrunA17_Chr6g0484481 | 45.122 | 164 | 68 | 7 | 5 | 163 | 21 | 167 | 1.28e-25 | 96.3 |
| Msa0043670 | MtrunA17_Chr1g0171841 | 38.931 | 131 | 60 | 4 | 26 | 149 | 31 | 148 | 4.39e-13 | 63.2 |
| Msa0043670 | MtrunA17_Chr4g0045631 | 38.732 | 142 | 58 | 5 | 9 | 149 | 22 | 135 | 1.47e-12 | 61.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0043670 | AT1G68490.1 | 59.756 | 164 | 53 | 6 | 4 | 163 | 29 | 183 | 4.03e-49 | 157 |
| Msa0043670 | AT1G13390.1 | 52.469 | 162 | 65 | 5 | 4 | 163 | 25 | 176 | 4.89e-42 | 138 |
| Msa0043670 | AT1G13390.2 | 52.469 | 162 | 65 | 5 | 4 | 163 | 25 | 176 | 4.89e-42 | 138 |
| Msa0043670 | AT5G16110.1 | 50.909 | 165 | 67 | 7 | 4 | 163 | 89 | 244 | 1.94e-35 | 124 |
| Msa0043670 | AT3G02555.2 | 46.296 | 162 | 56 | 5 | 5 | 163 | 29 | 162 | 2.16e-27 | 100 |
| Msa0043670 | AT3G02555.1 | 46.296 | 162 | 56 | 5 | 5 | 163 | 29 | 162 | 2.16e-27 | 100 |
Find 108 sgRNAs with CRISPR-Local
Find 307 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAATGATGTGATATTACGC+GGG | 0.699449 | 1_1:-75029737 | Msa0043670:five_prime_UTR |
| AAAGTAAAGAGGATGTTGTG+TGG | 0.752917 | 1_1:+75029538 | None:intergenic |
| AAATTCACCTGAGATGCCAC+CGG | 0.634810 | 1_1:+75028767 | None:intergenic |
| AACCACCATATAAGAGCTAC+GGG | 0.555576 | 1_1:-75027943 | Msa0043670:three_prime_UTR |
| AAGAAATGAAGAGGTGCAGG+TGG | 0.635822 | 1_1:+75029567 | None:intergenic |
| AAGGAAGGGAGGATGTGTTC+GGG | 0.568345 | 1_1:-75028124 | Msa0043670:CDS |
| AAGGATTCGATTGCCTTGAC+AGG | 0.436802 | 1_1:-75028068 | Msa0043670:CDS |
| AATAGAATGGGTTAATATCG+TGG | 0.530353 | 1_1:-75029766 | None:intergenic |
| AATCCGGCGGTGAGAGTCGA+AGG | 0.545936 | 1_1:-75028087 | Msa0043670:CDS |
| AATCCTTCGACTCTCACCGC+CGG | 0.616188 | 1_1:+75028084 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAATGATGTGATATTACGC+GGG | - | chr1_1:75027550-75027569 | Msa0043670:three_prime_UTR | 30.0% | |
| AATGTATGGTTAATTCATGC+GGG | + | chr1_1:75027584-75027603 | None:intergenic | 30.0% | |
| TAAATATAAAGCAACGAGTG+TGG | - | chr1_1:75027666-75027685 | Msa0043670:three_prime_UTR | 30.0% | |
| GAATTTACTGTACAAAGCTA+TGG | + | chr1_1:75027808-75027827 | None:intergenic | 30.0% | |
| CAGTAAATTCATTCAACACA+AGG | - | chr1_1:75027818-75027837 | Msa0043670:three_prime_UTR | 30.0% | |
| AGAAAAGAAAATTTCGACGA+AGG | - | chr1_1:75028405-75028424 | Msa0043670:intron | 30.0% | |
| ACACAAAGCAATTCAACAAA+AGG | + | chr1_1:75028889-75028908 | None:intergenic | 30.0% | |
| GACACTAGTACAAGAAATAA+GGG | + | chr1_1:75029567-75029586 | None:intergenic | 30.0% | |
| AGACACTAGTACAAGAAATA+AGG | + | chr1_1:75029568-75029587 | None:intergenic | 30.0% | |
| CTTACAAAGTTACAAACAAG+AGG | + | chr1_1:75029601-75029620 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1_1 | gene | 75027530 | 75029779 | 75027530 | ID=Msa0043670;Name=Msa0043670 |
| chr1_1 | mRNA | 75027530 | 75029779 | 75027530 | ID=Msa0043670-mRNA-1;Parent=Msa0043670;Name=Msa0043670-mRNA-1;_AED=0.16;_eAED=0.16;_QI=346|1|1|1|0.66|0.5|4|502|163 |
| chr1_1 | exon | 75027530 | 75028352 | 75027530 | ID=Msa0043670-mRNA-1:exon:25327;Parent=Msa0043670-mRNA-1 |
| chr1_1 | exon | 75028608 | 75028674 | 75028608 | ID=Msa0043670-mRNA-1:exon:25326;Parent=Msa0043670-mRNA-1 |
| chr1_1 | exon | 75028775 | 75028914 | 75028775 | ID=Msa0043670-mRNA-1:exon:25325;Parent=Msa0043670-mRNA-1 |
| chr1_1 | exon | 75029470 | 75029779 | 75029470 | ID=Msa0043670-mRNA-1:exon:25324;Parent=Msa0043670-mRNA-1 |
| chr1_1 | five_prime_UTR | 75029470 | 75029779 | 75029470 | ID=Msa0043670-mRNA-1:five_prime_utr;Parent=Msa0043670-mRNA-1 |
| chr1_1 | five_prime_UTR | 75028879 | 75028914 | 75028879 | ID=Msa0043670-mRNA-1:five_prime_utr;Parent=Msa0043670-mRNA-1 |
| chr1_1 | CDS | 75028775 | 75028878 | 75028775 | ID=Msa0043670-mRNA-1:cds;Parent=Msa0043670-mRNA-1 |
| chr1_1 | CDS | 75028608 | 75028674 | 75028608 | ID=Msa0043670-mRNA-1:cds;Parent=Msa0043670-mRNA-1 |
| chr1_1 | CDS | 75028032 | 75028352 | 75028032 | ID=Msa0043670-mRNA-1:cds;Parent=Msa0043670-mRNA-1 |
| chr1_1 | three_prime_UTR | 75027530 | 75028031 | 75027530 | ID=Msa0043670-mRNA-1:three_prime_utr;Parent=Msa0043670-mRNA-1 |
| Gene Sequence |
>Msa0043670 ATGAACGGAAGAGACTCCGTCGTTTGCCCTAAGCCCCGCCGTGTCGGACTCCTCAACTTCGCCGTCAACGACTACCCGAGCAGATCCTTCCGGTGGCATCTCAGTTGCCAAGTTGAACCGTGTGATTCAAACTCATCTGCGTCAAATCCCTTGGACACAATTCTCACCAAGGATGATGATTGTGATACGGAACAATCAAGTCCTCCAGTAGCCTCGTCGCCGCCCCCATTTTTCTGTGGGTCTCCGCCGAGCAGAGTAGCTAACCCACTAATTCAGGATGCTCGATTCGGGGATGAGAATTTCTCGCCCCTCTCACCGTCGTCATGGGTGGTGGTTCCTGCCCCATCCGGTCTGCCACCTTCTCCCCCATCTTCTGCAAGGAAGGGAGGATGTGTTCGGGCCAATTTTGGTAACAATCCGGCGGTGAGAGTCGAAGGATTCGATTGCCTTGACAGGGACAGCCGTAATTGCAGCATCCCTGCTCTGGCTTAG |
| Protein sequence |
>Msa0043670 MNGRDSVVCPKPRRVGLLNFAVNDYPSRSFRWHLSCQVEPCDSNSSASNPLDTILTKDDDCDTEQSSPPVASSPPPFFCGSPPSRVANPLIQDARFGDENFSPLSPSSWVVVPAPSGLPPSPPSSARKGGCVRANFGNNPAVRVEGFDCLDRDSRNCSIPALA* |
