Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1174340 | sp|Q9ZRA4|AB19A_PRUPE | 58.937 | 207 | 82 | 3 | 3 | 207 | 2 | 207 | 5.67e-78 | 235 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1174340 | G7L6V2 | 99.522 | 209 | 1 | 0 | 1 | 209 | 1 | 209 | 3.08e-148 | 419 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0458220 | Msa1174340 | 0.816015 | 6.873939e-52 | -8.615850e-47 |
| Msa0504700 | Msa1174340 | 0.828418 | 9.088651e-55 | -8.615850e-47 |
| Msa0873910 | Msa1174340 | 0.800050 | 1.728430e-48 | -8.615850e-47 |
| Msa0975960 | Msa1174340 | 0.800038 | 1.738152e-48 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1174340 | MtrunA17_Chr8g0343961 | 99.522 | 209 | 1 | 0 | 1 | 209 | 1 | 209 | 5.92e-152 | 419 |
| Msa1174340 | MtrunA17_Chr8g0343934 | 88.038 | 209 | 25 | 0 | 1 | 209 | 1 | 209 | 5.24e-134 | 374 |
| Msa1174340 | MtrunA17_Chr8g0343954 | 87.560 | 209 | 26 | 0 | 1 | 209 | 1 | 209 | 8.51e-127 | 356 |
| Msa1174340 | MtrunA17_Chr2g0301611 | 72.249 | 209 | 58 | 0 | 1 | 209 | 1 | 209 | 3.92e-106 | 303 |
| Msa1174340 | MtrunA17_Chr2g0302171 | 70.813 | 209 | 61 | 0 | 1 | 209 | 1 | 209 | 5.35e-104 | 298 |
| Msa1174340 | MtrunA17_Chr2g0313961 | 70.531 | 207 | 61 | 0 | 1 | 207 | 1 | 207 | 1.06e-102 | 295 |
| Msa1174340 | MtrunA17_Chr2g0297231 | 70.388 | 206 | 61 | 0 | 1 | 206 | 1 | 206 | 1.10e-102 | 295 |
| Msa1174340 | MtrunA17_Chr2g0297241 | 70.048 | 207 | 62 | 0 | 1 | 207 | 1 | 207 | 5.86e-101 | 290 |
| Msa1174340 | MtrunA17_Chr3g0084531 | 67.464 | 209 | 68 | 0 | 1 | 209 | 1 | 209 | 1.45e-98 | 285 |
| Msa1174340 | MtrunA17_Chr3g0084511 | 66.986 | 209 | 68 | 1 | 1 | 209 | 1 | 208 | 1.37e-95 | 277 |
| Msa1174340 | MtrunA17_Chr3g0084521 | 66.986 | 209 | 68 | 1 | 1 | 209 | 1 | 208 | 4.82e-95 | 275 |
| Msa1174340 | MtrunA17_Chr5g0411961 | 65.072 | 209 | 73 | 0 | 1 | 209 | 1 | 209 | 9.89e-94 | 272 |
| Msa1174340 | MtrunA17_Chr2g0302541 | 66.507 | 209 | 70 | 0 | 1 | 209 | 1 | 209 | 2.49e-91 | 266 |
| Msa1174340 | MtrunA17_Chr7g0217811 | 66.986 | 209 | 69 | 0 | 1 | 209 | 1 | 209 | 3.96e-90 | 263 |
| Msa1174340 | MtrunA17_Chr4g0012651 | 67.464 | 209 | 68 | 0 | 1 | 209 | 1 | 209 | 4.93e-90 | 263 |
| Msa1174340 | MtrunA17_Chr4g0003361 | 61.836 | 207 | 76 | 2 | 3 | 209 | 2 | 205 | 1.35e-85 | 251 |
| Msa1174340 | MtrunA17_Chr3g0084551 | 62.069 | 203 | 71 | 1 | 1 | 203 | 1 | 197 | 4.31e-80 | 238 |
| Msa1174340 | MtrunA17_Chr8g0343911 | 57.767 | 206 | 84 | 3 | 6 | 209 | 4 | 208 | 4.09e-79 | 235 |
| Msa1174340 | MtrunA17_Chr2g0300561 | 55.502 | 209 | 89 | 3 | 3 | 209 | 4 | 210 | 1.43e-76 | 229 |
| Msa1174340 | MtrunA17_Chr2g0300591 | 55.024 | 209 | 90 | 3 | 3 | 209 | 4 | 210 | 2.26e-76 | 228 |
| Msa1174340 | MtrunA17_Chr8g0343921 | 56.311 | 206 | 86 | 3 | 6 | 209 | 4 | 207 | 2.61e-76 | 228 |
| Msa1174340 | MtrunA17_Chr2g0300581 | 54.545 | 209 | 91 | 3 | 3 | 209 | 4 | 210 | 1.82e-75 | 226 |
| Msa1174340 | MtrunA17_Chr2g0286171 | 53.488 | 215 | 91 | 3 | 1 | 209 | 1 | 212 | 2.50e-75 | 226 |
| Msa1174340 | MtrunA17_Chr3g0115141 | 56.522 | 207 | 84 | 3 | 6 | 209 | 7 | 210 | 8.22e-75 | 224 |
| Msa1174340 | MtrunA17_Chr8g0344041 | 53.555 | 211 | 94 | 3 | 1 | 209 | 5 | 213 | 1.69e-74 | 224 |
| Msa1174340 | MtrunA17_Chr8g0344031 | 54.976 | 211 | 93 | 2 | 1 | 209 | 1 | 211 | 5.44e-74 | 222 |
| Msa1174340 | MtrunA17_Chr8g0344051 | 50.239 | 209 | 102 | 2 | 3 | 209 | 4 | 212 | 2.04e-69 | 211 |
| Msa1174340 | MtrunA17_Chr8g0344021 | 48.804 | 209 | 105 | 2 | 3 | 209 | 4 | 212 | 1.12e-63 | 196 |
| Msa1174340 | MtrunA17_Chr3g0084501 | 65.094 | 106 | 37 | 0 | 1 | 106 | 1 | 106 | 7.27e-44 | 142 |
| Msa1174340 | MtrunA17_Chr7g0271211 | 37.500 | 192 | 116 | 3 | 21 | 208 | 23 | 214 | 8.86e-37 | 127 |
| Msa1174340 | MtrunA17_Chr1g0193421 | 35.602 | 191 | 119 | 3 | 22 | 208 | 28 | 218 | 1.82e-35 | 124 |
| Msa1174340 | MtrunA17_Chr1g0193671 | 33.854 | 192 | 123 | 3 | 21 | 208 | 26 | 217 | 1.90e-33 | 119 |
| Msa1174340 | MtrunA17_Chr2g0292901 | 35.079 | 191 | 115 | 4 | 21 | 205 | 26 | 213 | 1.45e-31 | 114 |
| Msa1174340 | MtrunA17_Chr1g0189841 | 34.634 | 205 | 122 | 4 | 13 | 205 | 13 | 217 | 2.05e-31 | 114 |
| Msa1174340 | MtrunA17_Chr1g0210761 | 33.990 | 203 | 125 | 5 | 6 | 205 | 11 | 207 | 3.25e-31 | 113 |
| Msa1174340 | MtrunA17_Chr6g0449731 | 36.916 | 214 | 122 | 6 | 4 | 205 | 3 | 215 | 4.07e-31 | 113 |
| Msa1174340 | MtrunA17_Chr6g0449751 | 38.220 | 191 | 110 | 4 | 22 | 205 | 26 | 215 | 4.89e-31 | 113 |
| Msa1174340 | MtrunA17_Chr2g0292831 | 35.079 | 191 | 115 | 4 | 21 | 205 | 25 | 212 | 9.13e-31 | 112 |
| Msa1174340 | MtrunA17_Chr6g0449761 | 38.220 | 191 | 110 | 4 | 22 | 205 | 26 | 215 | 1.46e-30 | 112 |
| Msa1174340 | MtrunA17_Chr6g0449771 | 36.449 | 214 | 123 | 6 | 4 | 205 | 3 | 215 | 1.69e-30 | 111 |
| Msa1174340 | MtrunA17_Chr4g0007041 | 35.023 | 217 | 126 | 6 | 1 | 204 | 1 | 215 | 1.82e-30 | 111 |
| Msa1174340 | MtrunA17_Chr1g0178711 | 33.019 | 212 | 130 | 4 | 6 | 205 | 10 | 221 | 2.00e-30 | 111 |
| Msa1174340 | MtrunA17_Chr4g0007051 | 35.160 | 219 | 123 | 7 | 1 | 204 | 1 | 215 | 2.57e-30 | 111 |
| Msa1174340 | MtrunA17_Chr4g0007031 | 35.160 | 219 | 123 | 7 | 1 | 204 | 1 | 215 | 3.81e-30 | 110 |
| Msa1174340 | MtrunA17_Chr5g0419971 | 35.160 | 219 | 123 | 7 | 1 | 204 | 1 | 215 | 3.81e-30 | 110 |
| Msa1174340 | MtrunA17_Chr5g0419981 | 35.160 | 219 | 123 | 7 | 1 | 204 | 1 | 215 | 3.81e-30 | 110 |
| Msa1174340 | MtrunA17_Chr2g0285871 | 34.562 | 217 | 127 | 6 | 1 | 204 | 1 | 215 | 5.36e-30 | 110 |
| Msa1174340 | MtrunA17_Chr1g0156131 | 32.367 | 207 | 128 | 4 | 5 | 205 | 9 | 209 | 1.49e-29 | 108 |
| Msa1174340 | MtrunA17_Chr6g0449791 | 36.500 | 200 | 112 | 5 | 9 | 194 | 7 | 205 | 1.07e-27 | 104 |
| Msa1174340 | MtrunA17_Chr5g0414971 | 31.937 | 191 | 125 | 4 | 22 | 207 | 31 | 221 | 1.42e-27 | 104 |
| Msa1174340 | MtrunA17_Chr2g0292861 | 34.940 | 166 | 101 | 4 | 47 | 205 | 13 | 178 | 3.45e-26 | 99.4 |
| Msa1174340 | MtrunA17_Chr2g0321591 | 37.129 | 202 | 116 | 6 | 9 | 205 | 9 | 204 | 1.14e-25 | 99.0 |
| Msa1174340 | MtrunA17_Chr6g0449781 | 33.491 | 212 | 128 | 5 | 6 | 205 | 4 | 214 | 2.17e-25 | 98.6 |
| Msa1174340 | MtrunA17_Chr2g0321581 | 36.082 | 194 | 115 | 5 | 17 | 205 | 15 | 204 | 7.04e-25 | 96.7 |
| Msa1174340 | MtrunA17_Chr2g0292841 | 34.940 | 166 | 101 | 4 | 47 | 205 | 13 | 178 | 1.12e-24 | 95.5 |
| Msa1174340 | MtrunA17_Chr8g0335851 | 36.090 | 133 | 84 | 1 | 38 | 169 | 48 | 180 | 1.42e-24 | 96.3 |
| Msa1174340 | MtrunA17_Chr3g0130241 | 34.211 | 152 | 97 | 2 | 21 | 169 | 92 | 243 | 4.03e-24 | 96.7 |
| Msa1174340 | MtrunA17_Chr2g0292911 | 41.441 | 111 | 61 | 2 | 47 | 153 | 13 | 123 | 1.52e-23 | 91.7 |
| Msa1174340 | MtrunA17_Chr2g0321601 | 39.695 | 131 | 77 | 2 | 77 | 205 | 73 | 203 | 1.65e-22 | 90.5 |
| Msa1174340 | MtrunA17_Chr4g0026201 | 35.556 | 135 | 85 | 2 | 73 | 205 | 71 | 205 | 1.89e-18 | 79.7 |
| Msa1174340 | MtrunA17_Chr4g0026191 | 34.815 | 135 | 86 | 2 | 73 | 205 | 71 | 205 | 1.06e-17 | 77.8 |
| Msa1174340 | MtrunA17_Chr2g0327651 | 38.776 | 98 | 58 | 1 | 46 | 141 | 8 | 105 | 5.31e-17 | 73.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1174340 | AT1G72610.1 | 58.854 | 192 | 77 | 2 | 20 | 209 | 17 | 208 | 2.59e-76 | 228 |
| Msa1174340 | AT5G20630.1 | 54.545 | 209 | 93 | 2 | 1 | 207 | 1 | 209 | 3.57e-75 | 226 |
| Msa1174340 | AT5G39190.1 | 37.557 | 221 | 122 | 6 | 1 | 206 | 1 | 220 | 5.01e-40 | 136 |
| Msa1174340 | AT5G39160.3 | 38.140 | 215 | 117 | 6 | 3 | 206 | 7 | 216 | 1.14e-39 | 135 |
| Msa1174340 | AT5G39110.1 | 38.636 | 220 | 120 | 5 | 1 | 205 | 1 | 220 | 1.25e-39 | 135 |
| Msa1174340 | AT5G39160.1 | 38.140 | 215 | 121 | 6 | 3 | 206 | 7 | 220 | 1.66e-39 | 135 |
| Msa1174340 | AT5G39130.1 | 37.104 | 221 | 123 | 6 | 1 | 206 | 1 | 220 | 6.04e-39 | 134 |
| Msa1174340 | AT3G04200.1 | 38.679 | 212 | 110 | 7 | 12 | 206 | 15 | 223 | 3.36e-38 | 132 |
| Msa1174340 | AT3G62020.1 | 36.967 | 211 | 123 | 6 | 8 | 208 | 8 | 218 | 3.39e-38 | 132 |
| Msa1174340 | AT1G09560.1 | 38.889 | 198 | 117 | 3 | 12 | 205 | 18 | 215 | 4.14e-38 | 131 |
| Msa1174340 | AT3G05930.1 | 36.493 | 211 | 126 | 4 | 3 | 205 | 5 | 215 | 6.09e-38 | 131 |
| Msa1174340 | AT3G05950.1 | 39.713 | 209 | 110 | 6 | 12 | 205 | 14 | 221 | 8.30e-38 | 131 |
| Msa1174340 | AT1G18980.1 | 36.765 | 204 | 123 | 4 | 6 | 205 | 17 | 218 | 9.73e-38 | 130 |
| Msa1174340 | AT5G39180.1 | 37.900 | 219 | 122 | 5 | 1 | 205 | 1 | 219 | 5.08e-37 | 129 |
| Msa1174340 | AT5G39150.1 | 37.900 | 219 | 122 | 5 | 1 | 205 | 1 | 219 | 1.97e-36 | 127 |
| Msa1174340 | AT1G02335.1 | 35.961 | 203 | 124 | 4 | 12 | 208 | 16 | 218 | 2.50e-36 | 127 |
| Msa1174340 | AT5G39120.1 | 38.356 | 219 | 121 | 6 | 1 | 205 | 1 | 219 | 4.55e-36 | 126 |
| Msa1174340 | AT5G26700.1 | 35.122 | 205 | 130 | 3 | 6 | 208 | 9 | 212 | 5.73e-36 | 125 |
| Msa1174340 | AT5G39190.2 | 35.849 | 212 | 116 | 4 | 1 | 206 | 1 | 198 | 8.14e-36 | 125 |
| Msa1174340 | AT3G62020.2 | 38.068 | 176 | 104 | 4 | 38 | 208 | 14 | 189 | 1.83e-35 | 124 |
| Msa1174340 | AT5G39160.2 | 35.377 | 212 | 117 | 4 | 1 | 206 | 1 | 198 | 2.58e-35 | 124 |
| Msa1174340 | AT5G38960.1 | 36.548 | 197 | 114 | 5 | 20 | 206 | 28 | 223 | 4.08e-34 | 121 |
| Msa1174340 | AT1G18970.1 | 35.323 | 201 | 124 | 4 | 9 | 205 | 20 | 218 | 4.96e-33 | 118 |
| Msa1174340 | AT3G10080.1 | 37.755 | 196 | 104 | 7 | 22 | 205 | 30 | 219 | 2.16e-32 | 117 |
| Msa1174340 | AT3G04150.1 | 33.163 | 196 | 123 | 4 | 22 | 209 | 30 | 225 | 1.37e-31 | 115 |
| Msa1174340 | AT3G10080.2 | 37.755 | 196 | 104 | 7 | 22 | 205 | 101 | 290 | 1.57e-31 | 116 |
| Msa1174340 | AT4G14630.1 | 35.238 | 210 | 124 | 6 | 7 | 205 | 14 | 222 | 2.38e-31 | 114 |
| Msa1174340 | AT3G04150.2 | 34.444 | 180 | 111 | 3 | 37 | 209 | 59 | 238 | 2.71e-31 | 114 |
| Msa1174340 | AT5G38910.1 | 35.981 | 214 | 127 | 5 | 1 | 205 | 7 | 219 | 2.00e-30 | 112 |
| Msa1174340 | AT1G74820.1 | 34.225 | 187 | 117 | 4 | 23 | 205 | 41 | 225 | 3.75e-29 | 108 |
| Msa1174340 | AT5G61750.1 | 36.000 | 175 | 96 | 4 | 6 | 170 | 7 | 175 | 5.50e-29 | 107 |
| Msa1174340 | AT3G04170.1 | 33.803 | 213 | 121 | 7 | 7 | 205 | 12 | 218 | 1.60e-28 | 107 |
| Msa1174340 | AT5G38940.1 | 35.052 | 194 | 118 | 4 | 20 | 205 | 28 | 221 | 2.16e-28 | 106 |
| Msa1174340 | AT5G38940.2 | 35.052 | 194 | 118 | 4 | 20 | 205 | 52 | 245 | 3.95e-28 | 106 |
| Msa1174340 | AT5G38930.1 | 34.536 | 194 | 119 | 4 | 20 | 205 | 28 | 221 | 1.61e-27 | 104 |
| Msa1174340 | AT3G04190.1 | 31.959 | 194 | 124 | 4 | 20 | 205 | 28 | 221 | 1.75e-27 | 104 |
| Msa1174340 | AT3G04180.1 | 31.959 | 194 | 124 | 4 | 20 | 205 | 28 | 221 | 1.30e-25 | 99.4 |
| Msa1174340 | AT1G10460.1 | 36.220 | 127 | 78 | 3 | 60 | 183 | 65 | 191 | 1.97e-18 | 80.5 |
| Msa1174340 | AT5G38910.2 | 29.384 | 211 | 108 | 5 | 1 | 205 | 7 | 182 | 4.97e-15 | 70.5 |
Find 46 sgRNAs with CRISPR-Local
Find 55 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGATAGCCTGTAGGTGTTTC+TGG | 0.183963 | 8_1:-5431627 | None:intergenic |
| TGCTACCGAATTATTGATTA+TGG | 0.216281 | 8_1:+5431848 | Msa1174340:CDS |
| GAAAGAGTTGTTAGTGTTTC+CGG | 0.229368 | 8_1:-5431706 | None:intergenic |
| GAATTATTGATTATGGTTAA+AGG | 0.256071 | 8_1:+5431855 | Msa1174340:CDS |
| TAATTCAGAAGGTAAATTAT+TGG | 0.286814 | 8_1:-5432066 | None:intergenic |
| TTTCCAGCTCTTAACGATCT+TGG | 0.306429 | 8_1:+5431762 | Msa1174340:CDS |
| TAATTTACCTTCTGAATTAG+TGG | 0.324310 | 8_1:+5432070 | Msa1174340:CDS |
| GTTGAAACCGGGTGATCTTA+TGG | 0.324992 | 8_1:+5431926 | Msa1174340:CDS |
| AAGAAGCTAAAGGCTCGGTT+TGG | 0.389106 | 8_1:+5432125 | Msa1174340:CDS |
| TTATTCGAAGACGTTGAAAC+CGG | 0.390433 | 8_1:+5431914 | Msa1174340:CDS |
| TTTAACCATAATCAATAATT+CGG | 0.394261 | 8_1:-5431853 | None:intergenic |
| TCAGATGATTTCGTCTTTCA+CGG | 0.399030 | 8_1:+5431675 | Msa1174340:CDS |
| GTCTTTCACGGCTTTGTAGC+CGG | 0.402040 | 8_1:+5431687 | Msa1174340:CDS |
| AGAAGCTAAAGGCTCGGTTT+GGG | 0.424746 | 8_1:+5432126 | Msa1174340:CDS |
| TCTTCACTTGTGCAAGATCA+AGG | 0.425262 | 8_1:-5432106 | None:intergenic |
| ATCGTTAAGAGCTGGAAAAT+CGG | 0.453877 | 8_1:-5431757 | None:intergenic |
| TTTCAAGTTAATTCAGGTAA+GGG | 0.490682 | 8_1:+5431972 | Msa1174340:CDS |
| GTTAAAGGTGAAATAACTGC+TGG | 0.507279 | 8_1:+5431870 | Msa1174340:CDS |
| AGAGTGAAAAGGAAAAGTAT+AGG | 0.514106 | 8_1:-5431543 | None:intergenic |
| ATTCCAAGATCGTTAAGAGC+TGG | 0.523170 | 8_1:-5431765 | None:intergenic |
| ATCAATAATTCGGTAGCACC+AGG | 0.528604 | 8_1:-5431843 | None:intergenic |
| TATTCGAAGACGTTGAAACC+GGG | 0.533516 | 8_1:+5431915 | Msa1174340:CDS |
| AGATCAAGTAATTGAGCACC+AGG | 0.545978 | 8_1:-5432032 | None:intergenic |
| ATTCCAATGCATACTCATCC+TGG | 0.548743 | 8_1:+5431825 | Msa1174340:CDS |
| GCACCAGGATGAGTATGCAT+TGG | 0.553990 | 8_1:-5431828 | None:intergenic |
| GAAGCTAAAGGCTCGGTTTG+GGG | 0.563781 | 8_1:+5432127 | Msa1174340:CDS |
| AAAATCATGGACAGAAGCAT+AGG | 0.563955 | 8_1:-5431583 | None:intergenic |
| GTTTGTGCCACTAATTCAGA+AGG | 0.574335 | 8_1:-5432077 | None:intergenic |
| CATCTGATGTGATGTTTGCA+AGG | 0.576393 | 8_1:-5431659 | None:intergenic |
| AAGCTAAAGGCTCGGTTTGG+GGG | 0.585774 | 8_1:+5432128 | Msa1174340:CDS |
| TGCACAAGTGAAGAAGCTAA+AGG | 0.586569 | 8_1:+5432115 | Msa1174340:CDS |
| AAAGCTCCAGAAACACCTAC+AGG | 0.587844 | 8_1:+5431621 | Msa1174340:CDS |
| GACAGAAGCATAGGAAGTGG+TGG | 0.591518 | 8_1:-5431574 | None:intergenic |
| ATGGACAGAAGCATAGGAAG+TGG | 0.593850 | 8_1:-5431577 | None:intergenic |
| TGAAAATGCAACATCCCTTG+TGG | 0.594793 | 8_1:-5431954 | None:intergenic |
| ACTAAAAGTAAGAAAAGCAG+TGG | 0.611069 | 8_1:-5432000 | None:intergenic |
| ATCTGATGTGATGTTTGCAA+GGG | 0.619647 | 8_1:-5431658 | None:intergenic |
| TGGATGAGAGAAGAGTGAAA+AGG | 0.620855 | 8_1:-5431554 | None:intergenic |
| AAGTGAAGAAGCTAAAGGCT+CGG | 0.623995 | 8_1:+5432120 | Msa1174340:CDS |
| ATCTTGGAATCTCTGCGGCA+CGG | 0.632838 | 8_1:+5431778 | Msa1174340:CDS |
| GCACGGTTAGACATAGCGAA+AGG | 0.667594 | 8_1:+5431795 | Msa1174340:CDS |
| CGGTTAGACATAGCGAAAGG+CGG | 0.694402 | 8_1:+5431798 | Msa1174340:CDS |
| GGCATGCAGTGATAGCCTGT+AGG | 0.702564 | 8_1:-5431636 | None:intergenic |
| TAACGATCTTGGAATCTCTG+CGG | 0.726837 | 8_1:+5431773 | Msa1174340:CDS |
| TCTGATGTGATGTTTGCAAG+GGG | 0.732037 | 8_1:-5431657 | None:intergenic |
| AAAATCGGTGACAAACGCAG+AGG | 0.761602 | 8_1:-5431742 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTTAACCATAATCAATAATT+CGG | - | chr8_1:5431856-5431875 | None:intergenic | 15.0% |
| !!! | GAATTATTGATTATGGTTAA+AGG | + | chr8_1:5431855-5431874 | Msa1174340:CDS | 20.0% |
| !! | TAATTCAGAAGGTAAATTAT+TGG | - | chr8_1:5432069-5432088 | None:intergenic | 20.0% |
| !!! | TTGCATTTTCAAGTTAATTC+AGG | + | chr8_1:5431966-5431985 | Msa1174340:CDS | 25.0% |
| !!! | TTTTCAAGTTAATTCAGGTA+AGG | + | chr8_1:5431971-5431990 | Msa1174340:CDS | 25.0% |
| !!! | TTTCAAGTTAATTCAGGTAA+GGG | + | chr8_1:5431972-5431991 | Msa1174340:CDS | 25.0% |
| ! | TAATTTACCTTCTGAATTAG+TGG | + | chr8_1:5432070-5432089 | Msa1174340:CDS | 25.0% |
| ! | AGAGTGAAAAGGAAAAGTAT+AGG | - | chr8_1:5431546-5431565 | None:intergenic | 30.0% |
| TATCTCCTACACAAAAATCA+TGG | - | chr8_1:5431599-5431618 | None:intergenic | 30.0% | |
| ! | TGCTACCGAATTATTGATTA+TGG | + | chr8_1:5431848-5431867 | Msa1174340:CDS | 30.0% |
| ! | ATCTTATGGTTTTTCCACAA+GGG | + | chr8_1:5431940-5431959 | Msa1174340:CDS | 30.0% |
| ACTAAAAGTAAGAAAAGCAG+TGG | - | chr8_1:5432003-5432022 | None:intergenic | 30.0% | |
| ! | AAAATCATGGACAGAAGCAT+AGG | - | chr8_1:5431586-5431605 | None:intergenic | 35.0% |
| ! | TCTGTCCATGATTTTTGTGT+AGG | + | chr8_1:5431591-5431610 | Msa1174340:CDS | 35.0% |
| ATCTGATGTGATGTTTGCAA+GGG | - | chr8_1:5431661-5431680 | None:intergenic | 35.0% | |
| TCAGATGATTTCGTCTTTCA+CGG | + | chr8_1:5431675-5431694 | Msa1174340:CDS | 35.0% | |
| GAAAGAGTTGTTAGTGTTTC+CGG | - | chr8_1:5431709-5431728 | None:intergenic | 35.0% | |
| ATCGTTAAGAGCTGGAAAAT+CGG | - | chr8_1:5431760-5431779 | None:intergenic | 35.0% | |
| GTTAAAGGTGAAATAACTGC+TGG | + | chr8_1:5431870-5431889 | Msa1174340:CDS | 35.0% | |
| TTATTCGAAGACGTTGAAAC+CGG | + | chr8_1:5431914-5431933 | Msa1174340:CDS | 35.0% | |
| ! | GATCTTATGGTTTTTCCACA+AGG | + | chr8_1:5431939-5431958 | Msa1174340:CDS | 35.0% |
| ! | ACTTTTAGTAGTGCAAATCC+TGG | + | chr8_1:5432014-5432033 | Msa1174340:CDS | 35.0% |
| TGGATGAGAGAAGAGTGAAA+AGG | - | chr8_1:5431557-5431576 | None:intergenic | 40.0% | |
| TCTGATGTGATGTTTGCAAG+GGG | - | chr8_1:5431660-5431679 | None:intergenic | 40.0% | |
| CATCTGATGTGATGTTTGCA+AGG | - | chr8_1:5431662-5431681 | None:intergenic | 40.0% | |
| TTTCCAGCTCTTAACGATCT+TGG | + | chr8_1:5431762-5431781 | Msa1174340:CDS | 40.0% | |
| ATTCCAAGATCGTTAAGAGC+TGG | - | chr8_1:5431768-5431787 | None:intergenic | 40.0% | |
| TAACGATCTTGGAATCTCTG+CGG | + | chr8_1:5431773-5431792 | Msa1174340:CDS | 40.0% | |
| ATTCCAATGCATACTCATCC+TGG | + | chr8_1:5431825-5431844 | Msa1174340:CDS | 40.0% | |
| ATCAATAATTCGGTAGCACC+AGG | - | chr8_1:5431846-5431865 | None:intergenic | 40.0% | |
| TATTCGAAGACGTTGAAACC+GGG | + | chr8_1:5431915-5431934 | Msa1174340:CDS | 40.0% | |
| GGAAAAACCATAAGATCACC+CGG | - | chr8_1:5431936-5431955 | None:intergenic | 40.0% | |
| TGAAAATGCAACATCCCTTG+TGG | - | chr8_1:5431957-5431976 | None:intergenic | 40.0% | |
| AGATCAAGTAATTGAGCACC+AGG | - | chr8_1:5432035-5432054 | None:intergenic | 40.0% | |
| ! | GTTTGTGCCACTAATTCAGA+AGG | - | chr8_1:5432080-5432099 | None:intergenic | 40.0% |
| TCTTCACTTGTGCAAGATCA+AGG | - | chr8_1:5432109-5432128 | None:intergenic | 40.0% | |
| TGCACAAGTGAAGAAGCTAA+AGG | + | chr8_1:5432115-5432134 | Msa1174340:CDS | 40.0% | |
| AAGTGAAGAAGCTAAAGGCT+CGG | + | chr8_1:5432120-5432139 | Msa1174340:CDS | 40.0% | |
| ! | ATGGACAGAAGCATAGGAAG+TGG | - | chr8_1:5431580-5431599 | None:intergenic | 45.0% |
| AAAGCTCCAGAAACACCTAC+AGG | + | chr8_1:5431621-5431640 | Msa1174340:CDS | 45.0% | |
| TGATAGCCTGTAGGTGTTTC+TGG | - | chr8_1:5431630-5431649 | None:intergenic | 45.0% | |
| AAAATCGGTGACAAACGCAG+AGG | - | chr8_1:5431745-5431764 | None:intergenic | 45.0% | |
| GTTGAAACCGGGTGATCTTA+TGG | + | chr8_1:5431926-5431945 | Msa1174340:CDS | 45.0% | |
| ! | AAGAAGCTAAAGGCTCGGTT+TGG | + | chr8_1:5432125-5432144 | Msa1174340:CDS | 45.0% |
| ! | AGAAGCTAAAGGCTCGGTTT+GGG | + | chr8_1:5432126-5432145 | Msa1174340:CDS | 45.0% |
| ! | GACAGAAGCATAGGAAGTGG+TGG | - | chr8_1:5431577-5431596 | None:intergenic | 50.0% |
| ! | GTCTTTCACGGCTTTGTAGC+CGG | + | chr8_1:5431687-5431706 | Msa1174340:CDS | 50.0% |
| ATCTTGGAATCTCTGCGGCA+CGG | + | chr8_1:5431778-5431797 | Msa1174340:CDS | 50.0% | |
| GCACGGTTAGACATAGCGAA+AGG | + | chr8_1:5431795-5431814 | Msa1174340:CDS | 50.0% | |
| CGGTTAGACATAGCGAAAGG+CGG | + | chr8_1:5431798-5431817 | Msa1174340:CDS | 50.0% | |
| GCACCAGGATGAGTATGCAT+TGG | - | chr8_1:5431831-5431850 | None:intergenic | 50.0% | |
| ! | GAAGCTAAAGGCTCGGTTTG+GGG | + | chr8_1:5432127-5432146 | Msa1174340:CDS | 50.0% |
| !! | AAGCTAAAGGCTCGGTTTGG+GGG | + | chr8_1:5432128-5432147 | Msa1174340:CDS | 50.0% |
| GGCATGCAGTGATAGCCTGT+AGG | - | chr8_1:5431639-5431658 | None:intergenic | 55.0% | |
| !! | AAGGCTCGGTTTGGGGGAAG+AGG | + | chr8_1:5432134-5432153 | Msa1174340:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_1 | gene | 5431531 | 5432160 | 5431531 | ID=Msa1174340;Name=Msa1174340 |
| chr8_1 | mRNA | 5431531 | 5432160 | 5431531 | ID=Msa1174340-mRNA-1;Parent=Msa1174340;Name=Msa1174340-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|-1|0|1|-1|1|1|0|209 |
| chr8_1 | exon | 5431531 | 5432160 | 5431531 | ID=Msa1174340-mRNA-1:exon:1800;Parent=Msa1174340-mRNA-1 |
| chr8_1 | CDS | 5431531 | 5432160 | 5431531 | ID=Msa1174340-mRNA-1:cds;Parent=Msa1174340-mRNA-1 |
| Gene Sequence |
| Protein sequence |
